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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/948928.rdf" xlink:actuate="onRequest">Total mercury (THg) concentration, fork length, and mass data for bigeye tuna (Thunnus obesus), yellowfin tuna (Thunnus albacares), and longnose lancetfish (Alepisaurus ferox) collected from the central and eastern North Pacific between 1971 and 2023</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Chen, R. S., Paulson, E. T., Schartup, A. T., Choy, C. A. (2025) Total mercury (THg) concentration, fork length, and mass data for bigeye tuna (Thunnus obesus), yellowfin tuna (Thunnus albacares), and longnose lancetfish (Alepisaurus ferox) collected from the central and eastern North Pacific between 1971 and 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-01-22 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.948928.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Mercury concentrations fish 1971-2023 Dataset Description: &amp;lt;p&amp;gt;This dataset is referred to as &amp;quot;Dataset S2&amp;quot; in the supplementary material of Chen et al. The data were used in Figure 3.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Lancetfish Sample Collection:&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Whole lancetfish (n = 69) were collected by fisheries observers of the National Oceanic and Atmospheric Administration (NOAA) Pacific Islands Region and West Coast Region Observer Programs from 2018 to 2023 between 20 – 40° N and 115 – 160° W in the central and eastern North Pacific Ocean. Observers sampled lancetfish from the Hawaii-based shallow- and deep-set pelagic longline fisheries. Lancetfish were frozen whole at sea and capture location was recorded. In the lab, specimens were defrosted, measured to the nearest mm (FL), and weighed whole to the nearest 0.1 g.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Dorsal muscle tissue&amp;amp;nbsp;from each lancetfish&amp;amp;nbsp;was sampled&amp;amp;nbsp;posterior to the operculum at the base of the dorsal fin insertion and anterior to the vent. Only white muscle tissue was sampled, and skin, bones, and cartilage were removed from white muscle tissues before analysis. Following dissection, all tissues were gently rinsed with Milli-Q to avoid contamination between samples, placed in pre-weighed Whirl-Paks, and weighed before and after drying to measure moisture content. Tissues were frozen at -80˚C before being freeze-dried and homogenized within the Whirl-Pak or using an electronic mill (IKA Tube Mill 100 Control). Milling vessels and tools were cleaned with 95% ethanol between samples.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Tuna THg Data:&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;We used published size and THg data from muscle tissues of bigeye tuna (&amp;lt;em&amp;gt;Thunnus obesus&amp;lt;/em&amp;gt;) and yellowfin tuna (&amp;lt;em&amp;gt;Thunnus albacares&amp;lt;/em&amp;gt;) collected in the central North Pacific (Brooks, 2004; Choy et al., 2009; Kaneko and Ralston, 2007; Kraepiel et al., 2003; Rivers et al., 1972; Thieleke, 1973; as compiled in Drevnick and Brooks, 2017), along with data from Choy (2013). Life history, metabolism, and diet information for bigeye and yellowfin tuna are available in the literature and were used to explain changes in THg burden with size.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/943431.rdf" xlink:title="OCE-2414798" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2414798 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2414798</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/943640.rdf" xlink:title="P01ES035541" xlink:actuate="onRequest">Funding provided by National Institute of Environmental Health Sciences (NIEHS) Award Number: P01ES035541 Award URL: https://tools.niehs.nih.gov/portfolio/index.cfm?do=portfolio.grantDetail&amp;amp;grant_number=P01ES035541</gmx:Anchor>
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&amp;lt;p&amp;gt;Whole lancetfish (n = 69) were collected by fisheries observers of the National Oceanic and Atmospheric Administration (NOAA) Pacific Islands Region and West Coast Region Observer Programs from 2018 to 2023 between 20 – 40° N and 115 – 160° W in the central and eastern North Pacific Ocean. Observers sampled lancetfish from the Hawaii-based shallow- and deep-set pelagic longline fisheries. Lancetfish were frozen whole at sea and capture location was recorded. In the lab, specimens were defrosted, measured to the nearest mm (FL), and weighed whole to the nearest 0.1 g.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Dorsal muscle tissue&amp;amp;nbsp;from each lancetfish&amp;amp;nbsp;was sampled&amp;amp;nbsp;posterior to the operculum at the base of the dorsal fin insertion and anterior to the vent. Only white muscle tissue was sampled, and skin, bones, and cartilage were removed from white muscle tissues before analysis. Following dissection, all tissues were gently rinsed with Milli-Q to avoid contamination between samples, placed in pre-weighed Whirl-Paks, and weighed before and after drying to measure moisture content. Tissues were frozen at -80˚C before being freeze-dried and homogenized within the Whirl-Pak or using an electronic mill (IKA Tube Mill 100 Control). Milling vessels and tools were cleaned with 95% ethanol between samples.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Tuna THg Data:&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;We used published size and THg data from muscle tissues of bigeye tuna (&amp;lt;em&amp;gt;Thunnus obesus&amp;lt;/em&amp;gt;) and yellowfin tuna (&amp;lt;em&amp;gt;Thunnus albacares&amp;lt;/em&amp;gt;) collected in the central North Pacific (Brooks, 2004; Choy et al., 2009; Kaneko and Ralston, 2007; Kraepiel et al., 2003; Rivers et al., 1972; Thieleke, 1973; as compiled in Drevnick and Brooks, 2017), along with data from Choy (2013). Life history, metabolism, and diet information for bigeye and yellowfin tuna are available in the literature and were used to explain changes in THg burden with size.&amp;lt;/p&amp;gt;</gco:CharacterString>
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&amp;lt;p&amp;gt;Total mercury (THg) concentrations were determined using a Nippon Instruments MA-3000 Direct Mercury Analyzer (DMA), which uses direct thermal decomposition, gold amalgamation, and cold vapor atomic absorption spectroscopy. For quality assurance and quality control, certified reference materials National Research Council Canada (NRCC) DORM-4 (fish protein) and NRCC TORT-3 (lobster hepatopancreas) were analyzed at the beginning and end of each analysis day. Around 20 mg of dried, homogenized tissues were weighed out for THg analysis, and replicate samples and DORM-4 were analyzed every seven samples. A blank boat was combusted after samples expected to have high THg concentrations to reduce any potential THg carryover to subsequent samples. The average variation of replicate measurements was 4.1%, and analyses of DORM-4 (average measured THg = 417 ± 26 ng Hg/g dry weight, n = 161) and TORT-3 (296 ± 15 ng Hg/g dry weight, n = 40) were within 1.2% and 1.5% of mean certified values, respectively. Only data collected between standard reference materials that were within the range of certified values were included in analyses.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;All data processing was performed in R Statistical Software (version 4.3.2; R Core Team, 2023).&amp;lt;/p&amp;gt;</gco:CharacterString>
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