http://lod.bco-dmo.org/id/dataset/948929
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-01-21
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Total mercury and methylmercury concentrations measured across 10 tissues of the longnose lancetfish (Alepisaurus ferox) collected from the central and eastern North Pacific between 2018 and 2023
2025-01-22
publication
2025-01-22
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-02-10
publication
https://doi.org/10.26008/1912/bco-dmo.948929.1
C. Anela Choy
University of California-San Diego Scripps
principalInvestigator
Amina T. Schartup
University of California-San Diego Scripps
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Chen, R. S., Paulson, E. T., Schartup, A. T., Choy, C. A. (2025) Total mercury and methylmercury concentrations measured across 10 tissues of the longnose lancetfish (Alepisaurus ferox) collected from the central and eastern North Pacific between 2018 and 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-01-22 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.948929.1 [access date]
lancetfish 2018-2023 Dataset Description: <p>This dataset is referred to as "Dataset S1" in the supplementary material of Chen et al. The data were used in Figures 1 and 2, and Table 1.</p> Methods and Sampling: <p><strong>Sample Collection: </strong>Whole lancetfish (n = 69) were collected by fisheries observers of the National Oceanic and Atmospheric Administration (NOAA) Pacific Islands Region and West Coast Region Observer Programs from 2018 to 2023 between 20 – 40° N and 115 – 160° W in the central and eastern North Pacific Ocean. Observers sampled lancetfish from the Hawaii-based shallow- and deep-set pelagic longline fisheries. Lancetfish were frozen whole at sea and capture location was recorded. In the lab, specimens were defrosted, measured to the nearest mm (FL), and weighed whole to the nearest 0.1 g.</p>
<p>The following components, hereafter referred to as “tissues”, were dissected from each fish when available: brain, caudal muscle, dorsal muscle, gallbladder, gill filament, gonad, heart, intestine, liver, and stomach lining. Only white muscle tissue was sampled; dorsal muscle was sampled posterior to the operculum at the base of the dorsal fin insertion and anterior to the vent, and caudal muscle was sampled posterior to the adipose fin and anterior to the caudal peduncle. Skin, bones, and cartilage were removed from white muscle tissues before analysis. Gonads were sampled whole and were not distinguished as testes or ovaries as lancetfish larger than 100 cm are simultaneous hermaphrodites (Bañon et al. 2022). Stomachs were emptied and rinsed with Milli-Q water to remove all contents. Following dissection, all tissues were gently rinsed with Milli-Q to avoid contamination between samples, placed in pre-weighed Whirl-Paks, and weighed before and after drying to measure moisture content. Tissues were frozen at -80˚C before being freeze-dried and homogenized within the Whirl-Pak or using an electronic mill (IKA Tube Mill 100 Control). Milling vessels and tools were cleaned with 95% ethanol between samples.</p>
<p>&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2414798 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2414798
Funding provided by National Institute of Environmental Health Sciences (NIEHS) Award Number: P01ES035541 Award URL: https://tools.niehs.nih.gov/portfolio/index.cfm?do=portfolio.grantDetail&grant_number=P01ES035541
completed
C. Anela Choy
University of California-San Diego Scripps
808-822-3020
Scripps Institution of Oceanography 9500 Gilman Drive, MC 0218
La Jolla
CA
92093
USA
anela@ucsd.edu
pointOfContact
Amina T. Schartup
University of California-San Diego Scripps
858-534-8752
UC San Diego 9500 Gilman Dr #0220
La Jolla
CA
92093-0220
USA
aschartup@ucsd.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
specimen_id
date
latitude
longitude
FL_mm
whole_mass_g
size_class
tissue_type
percent_moisture
THg_wet_ng_g
hg_notes
MeHg_wet_ng_g
mehg_type
percent_mehg
Nippon Instruments MA-3000 Direct Mercury Analyzer
Tekran 2700 Methyl Mercury Auto-Analysis System
IKA Tube Mill 100 Control
Agilent 8900 triple quadrupole inductively coupled plasma mass spectrometer (ICP-MS)
LABCONCO Benchtop Freeze Dryer
Anton Paar Multiwave 5000
theme
None, User defined
sample identification
date
latitude
longitude
fish_len
wet_weight_biomass
taxonomic class
percent composition
total mercury
comments
methylmercury
featureType
BCO-DMO Standard Parameters
Automated Mercury Analysis System
Automated Mercury Analysis System
Homogenizer
Inductively Coupled Plasma Mass Spectrometer
Lyophilizer
Microwave Digestion Platform
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Scripps Center for Oceans and Human Health: advancing the science of marine contaminants and seafood security
https://osprey.bco-dmo.org/project/943432
Scripps Center for Oceans and Human Health: advancing the science of marine contaminants and seafood security
<p>The Scripps Center for Oceans and Human Health (SCOHH) is a five-year effort to advance the science and community engagement surrounding seafood pollutants, on a rapidly changing planet. The project brings together a multidisciplinary team of biomedical and oceanographic researchers with expertise in fish ecology, microbiology, marine chemistry, climate modeling, technology development, bioaccumulation, genomics, toxicology, and public health. The Center?s scientific goals and focus are guided by the needs of society, established through bidirectional community engagement, and led by a proven community engagement team. The proposed research program of SCOHH spans four main areas:</p>
<p>1. Climate change impacts on the human intake of seafood micronutrients and contaminants.<br />
2. The marine microbiome as a source for the synthesis, transformation, and distribution of seafood contaminants.<br />
3. Mechanisms of bioaccumulation and developmental toxicity of seafood pollutants.<br />
4. Bidirectional public engagement and literacy surrounding seafood risks and benefits.</p>
<p>The outcomes of the SCOHH will inform policies, consumption guidelines, and individual decisions to lower risk and enhance greater benefits associated with seafood consumption. Internally, SCOHH will take deliberate measures to enhance engagement with community partners. The Center is jointly supported by NSF?s Division of Ocean Sciences and by the National Institute for Environmental Health Sciences (NIEHS).</p>
<p>The central scientific theme of SCOHH is to advance knowledge of marine contaminants and seafood security. Natural and anthropogenic contaminants such as mercury, DDT, and PCBs drive seafood consumption advisories. Yet understanding of their sources, microbial transformations, toxicity, and potential for climate driven change remain incomplete. The SCOHH team will study and track the distribution of essential micronutrients and harmful contaminants in marine food webs to the three billion people who consume seafood globally, the roles that the marine microbiome play in their production and transport, and the developmental toxicity of seafood pollutants and their interactions with transporters that determine uptake and bioaccumulation.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
SCOHH
largerWorkCitation
project
eng; USA
oceans
-160
-115
20
40
2018-01-01
2023-09-30
Scripps Center for Oceans and Human Health
0
BCO-DMO catalogue of parameters from Total mercury and methylmercury concentrations measured across 10 tissues of the longnose lancetfish (Alepisaurus ferox) collected from the central and eastern North Pacific between 2018 and 2023
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/949128.rdf
Name: specimen_id
Units: unitless
Description: <p>unique identifier assigned to individual fish</p>
http://lod.bco-dmo.org/id/dataset-parameter/949129.rdf
Name: date
Units: unitless
Description: <p>fish collection date, in the format YYYY-MM</p>
http://lod.bco-dmo.org/id/dataset-parameter/949130.rdf
Name: latitude
Units: degrees
Description: <p>fish collection location. Location data are provided in 5x5 degree cells. The coordinate represents the lower left corner of the 5x5 degree cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/949131.rdf
Name: longitude
Units: degrees
Description: <p>fish collection location. Location data are provided in 5x5 degree cells. The coordinate represents the lower left corner of the 5x5 degree cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/949132.rdf
Name: FL_mm
Units: millimeters (mm)
Description: <p>fork length of whole fish</p>
http://lod.bco-dmo.org/id/dataset-parameter/949133.rdf
Name: whole_mass_g
Units: grams (g)
Description: <p>wet weight of whole fish</p>
http://lod.bco-dmo.org/id/dataset-parameter/949134.rdf
Name: size_class
Units: unitless
Description: <p>lancetfish size class, where individuals <1.8 kg are considered "small" and individuals ≥1.8 kg are considered "large"</p>
http://lod.bco-dmo.org/id/dataset-parameter/949135.rdf
Name: tissue_type
Units: unitless
Description: <p>tissue type analyzed</p>
http://lod.bco-dmo.org/id/dataset-parameter/949136.rdf
Name: percent_moisture
Units: percentage (%)
Description: <p>moisture content of the tissue</p>
http://lod.bco-dmo.org/id/dataset-parameter/949137.rdf
Name: THg_wet_ng_g
Units: nanogram per gram (ng/g)
Description: <p>THg concentration reported as ng Hg/g on a wet weight basis</p>
http://lod.bco-dmo.org/id/dataset-parameter/949138.rdf
Name: hg_notes
Units: unitless
Description: <p>notes on whether measurements are averaged from replicate measurements of the same sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/949139.rdf
Name: MeHg_wet_ng_g
Units: nanogram per gram (ng/g)
Description: <p>methylmercury (MeHg) concentration reported as ng Hg/g on a wet weight basis</p>
http://lod.bco-dmo.org/id/dataset-parameter/949140.rdf
Name: mehg_type
Units: unitless
Description: <p>"measured" or "predicted" to distinguish between measured MeHg concentrations and MeHg concentrations predicted with a generalized additive model using percent MeHg and mass</p>
http://lod.bco-dmo.org/id/dataset-parameter/949141.rdf
Name: percent_mehg
Units: percentage (%)
Description: <p>MeHg content of the tissue, as a percentage of THg content</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
54067
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/6d86774d-14cc-4459-bdfe-e7399a8cd80e/content
download
https://doi.org/10.26008/1912/bco-dmo.948929.1
download
onLine
dataset
<p><strong>Sample Collection: </strong>Whole lancetfish (n = 69) were collected by fisheries observers of the National Oceanic and Atmospheric Administration (NOAA) Pacific Islands Region and West Coast Region Observer Programs from 2018 to 2023 between 20 – 40° N and 115 – 160° W in the central and eastern North Pacific Ocean. Observers sampled lancetfish from the Hawaii-based shallow- and deep-set pelagic longline fisheries. Lancetfish were frozen whole at sea and capture location was recorded. In the lab, specimens were defrosted, measured to the nearest mm (FL), and weighed whole to the nearest 0.1 g.</p>
<p>The following components, hereafter referred to as “tissues”, were dissected from each fish when available: brain, caudal muscle, dorsal muscle, gallbladder, gill filament, gonad, heart, intestine, liver, and stomach lining. Only white muscle tissue was sampled; dorsal muscle was sampled posterior to the operculum at the base of the dorsal fin insertion and anterior to the vent, and caudal muscle was sampled posterior to the adipose fin and anterior to the caudal peduncle. Skin, bones, and cartilage were removed from white muscle tissues before analysis. Gonads were sampled whole and were not distinguished as testes or ovaries as lancetfish larger than 100 cm are simultaneous hermaphrodites (Bañon et al. 2022). Stomachs were emptied and rinsed with Milli-Q water to remove all contents. Following dissection, all tissues were gently rinsed with Milli-Q to avoid contamination between samples, placed in pre-weighed Whirl-Paks, and weighed before and after drying to measure moisture content. Tissues were frozen at -80˚C before being freeze-dried and homogenized within the Whirl-Pak or using an electronic mill (IKA Tube Mill 100 Control). Milling vessels and tools were cleaned with 95% ethanol between samples.</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
<p><strong>Analytical Methods: </strong>Total mercury (THg) concentrations were determined using a Nippon Instruments MA-3000 Direct Mercury Analyzer (DMA), which uses direct thermal decomposition, gold amalgamation, and cold vapor atomic absorption spectroscopy. For quality assurance and quality control, certified reference materials National Research Council Canada (NRCC) DORM-4 (fish protein) and NRCC TORT-3 (lobster hepatopancreas) were analyzed at the beginning and end of each analysis day. Around 20 mg of dried, homogenized tissues were weighed out for THg analysis, and replicate samples and DORM-4 were analyzed every seven samples. A blank boat was combusted after samples expected to have high THg concentrations (e.g., livers from large fish) to reduce any potential THg carryover to subsequent samples. The average variation of replicate measurements was 4.1%, and analyses of DORM-4 (average measured THg = 417 ± 26 ng Hg/g dry weight, n = 161) and TORT-3 (296 ± 15 ng Hg/g dry weight, n = 40) were within 1.2% and 1.5% of mean certified values, respectively. Only data collected between standard reference materials that were within the range of certified values were included in analyses.</p>
<p>We analyzed tissues known to play roles in methylmercury (MeHg) accumulation and excretion for MeHg. We selected sets of dorsal muscle, stomach lining, intestine, and liver samples from 10 lancetfish representing a range of masses (116.0 – 3920.8 g) for MeHg analysis (n = 40 samples total). We also analyzed brains from 33 whole lancetfish for MeHg. MeHg concentrations were determined using species-specific isotope dilution mass spectrometry following microwave-assisted acid digestion. This method quantifies the MeHg concentration of a sample based on the recovery of an isotopically labeled&nbsp;Me<sup>201</sup>Hg&nbsp;solution added during sample preparation. A stock&nbsp;Me<sup>201</sup>Hg&nbsp;solution was synthesized from <sup>201</sup>Hg<sup>II</sup> (96.3% purity) (Gilmour and Riedel, 1995; Hintelmann et al., 2000; Hintelmann and Ogrinc, 2002) and diluted to a 13 ng/mL working solution. The MeHg concentration of the&nbsp;Me<sup>201</sup>Hg&nbsp;working solution was periodically quantified using reverse isotope dilution with standards prepared from a certified 1000 ppm MeHg (II) chloride standard (Alfa Aesar). Exact concentrations were used to calculate MeHg concentrations in the samples after signal deconvolution of&nbsp;Me<sup>201</sup>Hg&nbsp;and&nbsp;Me<sup>202</sup>Hg&nbsp;peak areas.&nbsp;</p>
<p>To prepare samples for analysis, around 20 mg of tissue were weighed into acid-cleaned 50 mL PTFE-TFM pressure vessels with 10 mL of 5 M nitric acid and 85 µL of the 13 ng/mL&nbsp;Me<sup>201</sup>Hg&nbsp;working solution. The volume of&nbsp;Me<sup>201</sup>Hg&nbsp;added was selected to target a&nbsp;<sup>202</sup>Hg/<sup>201</sup>Hg&nbsp;mixing ratio of 0.23 to minimize uncertainty in signal deconvolution (Heimbürger et al., 2015; Rousseau et al., 2013). Samples were digested using an Anton Paar Multiwave 5000, which heated the samples to 70˚C for 10 minutes after a 5-minute ramp-up period. After digestion, sample digestates were diluted with 30 mL of Milli-Q water and stored at 4˚C.&nbsp;</p>
<p>MeHg in each sample was derivatized for analysis using ascorbic acid-assisted direct ethylation (Munson et al., 2014). 100 µL of sample digestate were added to a clean 60 mL amber borosilicate glass vial with 45 mL of Milli-Q water. Sample pH was adjusted to 4.8 using 2 M acetate/glacial acetic acid buffer before ascorbic acid (2.5% w/v) was added. Samples were ethylated with sodium tetraethylborate (1% NaTEB in 2% potassium hydroxide), and ethylation was allowed to proceed for at least 10 minutes before analysis. All reagents were prepared using Milli-Q water as needed. Samples were purged with Hg-free ultra-high purity argon gas, volatile Hg species were separated via gas chromatography using a Tekran 2700 Methyl Mercury Auto-Analysis System, and Me<sup>201</sup>Hg and Me<sup>202</sup>Hg were detected using an Agilent 8900 triple quadrupole inductively coupled plasma mass spectrometer (ICP-MS). Replicate samples, DORM-4, and TORT-3 were analyzed in each batch prepared for microwave digestion. The average variation of replicate measurements was 6.7%, and analyses of DORM-4 (average measured MeHg = 363 ± 24 ng Hg/g dry weight, n = 9) and TORT-3 (128 ± 8 ng Hg/g dry weight, n = 9) were within 2.3% and 6.9% of mean certified values, respectively.&nbsp;</p>
<p><strong>Data Analysis:&nbsp; </strong>Because MeHg analysis is costly and time-consuming, we analyzed a subset of tissues for MeHg to confirm the relationship between THg and MeHg concentrations. THg and MeHg concentrations were highly correlated in dorsal muscle, liver, stomach lining, and intestine (Spearman’s rank correlation, r = 1.00, 0.99, 0.98, and 0.83, respectively). However, we analyzed all brains for MeHg, so comparisons of measured MeHg concentrations between tissues were skewed by uneven sample size. To address this issue, we fit a generalized additive model (GAM) using restricted maximum likelihood estimation, a gaussian family distribution, and an “identity” link function using the mgcv package (version 1.9.0; Wood, 2011) to predict percent MeHg from lancetfish mass by tissue type using paired MeHg and THg measurements (Table S1). Percent MeHg was calculated as the MeHg concentration divided by the THg concentration of the same sample. We then used the GAM to estimate MeHg concentrations of dorsal muscle, stomach lining, intestine, and liver samples from fish with measured brain MeHg concentrations. We only measured MeHg in brain due to low sample mass, and thus do not report percent MeHg.</p>
<p>All mercury concentrations are reported in ng Hg/g on a wet-weight basis using percent moisture data obtained from the freeze-drying process. All data processing was performed in R Statistical Software (version 4.3.2; R Core Team, 2023).</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Nippon Instruments MA-3000 Direct Mercury Analyzer
Nippon Instruments MA-3000 Direct Mercury Analyzer
PI Supplied Instrument Name: Nippon Instruments MA-3000 Direct Mercury Analyzer PI Supplied Instrument Description:Nippon Instruments MA-3000 Direct Mercury Analyzer: used to collect total mercury concentration data from lancetfish tissues Instrument Name: Automated Mercury Analysis System Instrument Short Name: Instrument Description: Examples include Tekran Models 2600 and 2700
Tekran 2700 Methyl Mercury Auto-Analysis System
Tekran 2700 Methyl Mercury Auto-Analysis System
PI Supplied Instrument Name: Tekran 2700 Methyl Mercury Auto-Analysis System PI Supplied Instrument Description:Tekran 2700 Methyl Mercury Auto-Analysis System: uses gas chromatography to separate mercury species in samples Instrument Name: Automated Mercury Analysis System Instrument Short Name: Instrument Description: Examples include Tekran Models 2600 and 2700
IKA Tube Mill 100 Control
IKA Tube Mill 100 Control
PI Supplied Instrument Name: IKA Tube Mill 100 Control PI Supplied Instrument Description:IKA Tube Mill 100 Control: used to homogenize lancetfish tissues Instrument Name: Homogenizer Instrument Short Name:Homogenizer Instrument Description: A homogenizer is a piece of laboratory equipment used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others.
Agilent 8900 triple quadrupole inductively coupled plasma mass spectrometer (ICP-MS)
Agilent 8900 triple quadrupole inductively coupled plasma mass spectrometer (ICP-MS)
PI Supplied Instrument Name: Agilent 8900 triple quadrupole inductively coupled plasma mass spectrometer (ICP-MS) PI Supplied Instrument Description:Agilent 8900 triple quadrupole inductively coupled plasma mass spectrometer (ICP-MS): used to detect Me201Hg and Me202Hg to quantify methylmercury concentrations Instrument Name: Inductively Coupled Plasma Mass Spectrometer Instrument Short Name:ICP Mass Spec Instrument Description: An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB15/
LABCONCO Benchtop Freeze Dryer
LABCONCO Benchtop Freeze Dryer
PI Supplied Instrument Name: LABCONCO Benchtop Freeze Dryer PI Supplied Instrument Description:LABCONCO Benchtop Freeze Dryer: used to freeze dry tissues Instrument Name: Lyophilizer Instrument Short Name:freeze dryer Instrument Description: A lyophilizer, also known as freeze dryer or liofilizador, is a device that is used to freeze-dry material.
Anton Paar Multiwave 5000
Anton Paar Multiwave 5000
PI Supplied Instrument Name: Anton Paar Multiwave 5000 PI Supplied Instrument Description:Anton Paar Multiwave 5000: microwave used to digest tissues Instrument Name: Microwave Digestion Platform Instrument Short Name: Instrument Description: Microwave digestion is a chemical technique used to decompose sample material into a solution suitable for quantitative elemental analysis