http://lod.bco-dmo.org/id/dataset/949788 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2025-01-28 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Incubation experiments to assess the physiological and molecular response of subsurface phytoplankton communities to a simulation of the Upwelling Conveyor Belt Cycle (UCBC) conducted in summer 2019 onboard the R/V Oceanus (OC1905b) 2025-02-19 publication 2025-02-19 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2025-08-07 publication https://doi.org/10.26008/1912/bco-dmo.949788.1 Adrian Marchetti University of North Carolina at Chapel Hill principalInvestigator Matthew P. Hurst Cal Poly Humboldt (formerly Humboldt State University) principalInvestigator YuanYu Lin University of North Carolina at Chapel Hill principalInvestigator Claire P. Till Cal Poly Humboldt (formerly Humboldt State University) principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Lin, Y., Marchetti, A., Till, C. P., Hurst, M. P. (2025) Incubation experiments to assess the physiological and molecular response of subsurface phytoplankton communities to a simulation of the Upwelling Conveyor Belt Cycle (UCBC) conducted in summer 2019 onboard the R/V Oceanus (OC1905b). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-02-19 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.949788.1 [access date] PUPCYCLE 2019 incubation experiments Dataset Description: Methods and Sampling: &lt;p&gt;&lt;strong&gt;For the Fe status incubations:&lt;/strong&gt;&lt;br /&gt; Seawater was collected using trace-metal clean techniques from a depth of 90 meters (m) (corresponding to the 8.4 degree Celsius isotherm) for the wide shelf incubation and 80 m (corresponding to the 8.9 degree Celsius isotherm) for the narrow shelf incubation. Seawater from both sites was pumped directly into a positive pressure trace-metal clean plastic bubble created in the ship’s laboratory into large, 50-gallon acid-washed high-density polyethylene (HDPE) drums to homogenize the seawater using a Wilden air-operated double-diaphragm pump made of polytetrafluoroethylene and acid-washed HDPE tubing. Preparation of the cubitainers was carried out prior to the cruise and included trace-metal clean techniques. Cubitainers were initially soaked in 2% Extran detergent for 7 days, then rinsed with deionized water four times and Milli-Q water three times prior to being soaked in 10% reagent grade hydrochloric acid for 2-3 days and rinsed with Milli-Q water. Subsequently, the cubitainers were soaked in 1% trace metal grade hydrochloric acid for 7 days and rinsed with Milli-Q water, then soaked in 0.1% trace metal grade acetic acid 3-4 days before final storage in low Fe water from Station P (50 degrees North,145 degrees West). Station P water had been collected and filtered from the 2018 EXPORTS North Pacific field campaign using trace metal clean techniques and stored in the dark at 4 degrees Celsius (°C) until use. In the bubble, these triplicate acid-cleaned 10-liter (L) low-density polyethylene cubitainers were filled and then incubated in large on-deck plexiglass incubators circulated with water chilled to the temperatures at which the subsurface samples were collected using Aqua Logic Delta Star® In-Line Water Chillers. Incubators were covered with neutral density screening to achieve 30% incident irradiance. To assess the effects of Fe addition or removal on the simulated upwelled plankton communities, samples were incubated with no amendment (control), amended with 5 nanomolar (nM) of FeCl2 (Fe treatment), or amended with 200 nM desferrioxamine B (DFB), a strong Fe chelator, which inhibits dissolved Fe uptake (DFB treatment). Three cubitainers were immediately harvested for the initial time- point (T0), also referred to as deep-water or DW. The remaining cubitainers were incubated for two additional time points for a total of 18 cubitainers per incubation. Cubitainers were harvested for various biological and chemical parameters (see below) following 48 hours (T1) for both incubation experiments, and following 120 hours in the wide shelf incubation and 96 hours in the narrow shelf incubation (T2).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;For the seed population incubations:&lt;/strong&gt;&lt;br /&gt; Seawater was collected using trace-metal clean techniques at 15 m depth for the surface incubations and at 90 m for the subsurface incubations (corresponding to the 8.4 °C isotherm). Seawater was pumped directly into a positive pressure trace metal clean plastic bubble created in the ship's laboratory into large, 50-gallon acid-washed high-density polyethylene (HDPE) drums to homogenize the seawater using a Wilden air-operated double-diaphragm pump made of polytetrafluoroethylene (PTFE) and acid-washed HDPE tubing. Preparation of the cubitainers was carried out prior to the cruise and included trace-metal clean techniques (Crawford et al., 2003): 10 L cubitainers were initially soaked in 2% Extran detergent for 7 days, then rinsed with deionized water four times and Milli-Q water three times prior to being soaked in 10% reagent grade hydrochloric acid for 2-3 days and rinsed with Milli-Q water. Subsequently, the cubitainers were soaked in 1% trace metal grade hydrochloric acid for 7 days and rinsed with Milli-Q water, then soaked in 0.1% trace metal grade acetic acid 3-4 days before final storage in low-Fe water from Ocean Station Papa (St. P). The St. P water had been collected and filtered from the 2018 EXPORTS North Pacific (50 °N, 145 °W) field campaign using trace metal clean techniques and stored in the dark at 4 °C until use. In the bubble, these triplicate acid-cleaned 10 L low-density polyethylene (LDPE) cubitainers were filled and then incubated in large on-deck plexiglass incubators circulated with water chilled to the temperatures at which the subsurface samples were collected using Aqua Logic Delta Star® In-Line Water Chillers. Incubators were covered with neutral density screening to achieve 30% incident irradiance. For the experimental design, a portion of the surface and subsurface collections was directly collected and denoted as the initial (T0) surface and subsurface communities, respectively. The resulting incubations (triplicates) were characterized by either the surface waters (SW) or the subsurface waters (DW) incubated for 96 hours (T1). Another portion (triplicates) was subjected to mixture with filtered waters from the respective subsurface or surface communities, such that the 96 hour incubations consisted of an equal mixture of filtered-subsurface waters and surface waters, denoted as FDW/SW (filtered deep water/surface water), an equal mixture of filtered-surface waters and subsurface waters, denoted as FSW/DW (filtered surface water/deep water), and an equal mixture of both unfiltered surface and subsurface waters, denoted as mixed (surface water/deep water). No additional macronutrients or micronutrients were added to any of the incubations. Cubitainers were harvested for various biological and chemical parameters (see below) in the initial time point and following 96 hours of incubation.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Physiological Measurements:&lt;/strong&gt;&lt;br /&gt; For chlorophyll a measurements, 250 milliliters (mL) of seawater was gravity filtered through 5-micrometer (μm) Isopore membrane filters (47-millimeters (mm)) and subsequently vacuum filtered onto GF/F (25 mm) filters under 100 mmHg of vacuum pressure. Filters were then rinsed with 0.45 μm filtered seawater and immediately stored at -20 °C until onshore analysis in the lab. Chlorophyll a extraction was performed using a 90% acetone solution at -20 °C for 24 hours and measured on a 10-AU fluorometer (Turner Designs, San Jose, CA) using the acidification method.&lt;/p&gt; &lt;p&gt;Dissolved inorganic nutrients (nitrate + nitrite, phosphate, and silicic acid) were measured by filtering 30 mL of water through a 0.2 µm filter, using acid-washed syringes into an acid-cleaned polypropylene FalconTM tube. Dissolved nutrient concentrations were analyzed using an OI Analytical Flow Solutions IV auto analyzer by Wetland Biogeochemistry Analytical Services at Louisiana State University. Concentrations of nitrate measured from the discrete samples were used for the calculation of absolute nitrate uptake rates.&lt;/p&gt; &lt;p&gt;Samples for dissolved iron (dFe) were collected from each cubitainer within a trace-metal clean, positive pressure plastic bubble by filtering through pre-cleaned 0.2 μm pore size polyethersulfone membrane Acropak-200® capsule filters into LDPE bottles that had been rigorously cleaned as described in the GEOTRACES cookbook. Sample bottles were rinsed three times with sample before filling. Samples were acidified at sea to pH ~1.7 with optima HCl (2 mL of 12 M HCl per liter of seawater) and were analyzed after the cruise. Briefly, this method involves pre-concentration onto Nobias-chelate PA1 resin followed by analysis with a High-Resolution Inductively Coupled Plasma Mass Spectrometer. For quality control, a few samples were rerun with a flow injection analysis method. This method involves pre-concentration on toyopearl resin followed by in-line spectrophotometric analysis.&lt;/p&gt; &lt;p&gt;To assess the isotope uptake of DIC and NO3, a 618 mL polycarbonate bottle was filled to the top with incubation samples from each cubitainer. The subsamples were collected for dissolved inorganic carbon (DIC) and nitrate uptake rates at each respective time point, and immediately spiked with both NaH13CO3 and Na15NO3 at approximately 10% of the estimated ambient DIC concentrations and measured nitrate concentrations in a trace metal clean (TMC) space located on the ship (see below). Since there were no underway measurements of DIC, an expected ambient concentration of 2000 micromoles per liter (μmol L-1) was derived from literature values for both incubation sites. Nitrate concentrations of the deep-water and surface water in both incubation sites were measured using the Submersible Ultraviolet Nitrate Analyzer (SUNA), which quantifies dissolved nitrate concentrations by illuminating the water sample with UV and using the absorbance values to estimate nitrate concentrations from a multi-variable linear regression based on the MBARI method. After spiking with respective trace concentrations of both stable isotopes of DIC and nitrate, samples were returned to the incubator for six hours. All rate measurement incubations were initiated at approximately the same time of day (i.e., close to dawn). Following incubation, seawater was immediately filtered where cells greater than 5 μm were gravity filtered onto 5 μm Isopore membrane filters (47 mm) and then washed onto a pre-combusted (450 °C for 5 hours) GF/F filters (0.7 μm nominal porosity) by vacuum filtration using 0.2 μm filtered seawater and preserved at -20 °C. Cells smaller than 5 μm passed through the initial 5 μm Isopore membrane filters and were collected on pre-combusted GF/F filters and preserved at -20 °C. Prior to analysis, filters were dried at 60 °C for 24 hours, encapsulated in tin and pelletized. Particulate organic carbon (POC), particulate organic nitrogen (PON), and atom percentages of 13C and 15N were subsequently quantified using an isotope ratio mass spectrometer (EA-IRMS) at the UC Davis Stable Isotope Facility. For each sample, POC and PON concentrations (μmol L-1) were calculated by dividing the measured POC/PON mass (micrograms (μg)) by the respective atomic mass of carbon and nitrogen over the volume filtered (0.62 L).&lt;/p&gt; &lt;p&gt;For the wide shelf incubation, samples were spiked with 200 μmol L-1 of NaH13CO3 and 1 μmol L-1 of Na15NO3 according to estimated ambient DIC concentrations of 2000 μmol L-1 and measured surface nitrate concentrations of 10 μmol L-1. For the narrow shelf incubation, samples were spiked with 200 μmol L-1 of NaH13CO3 for all samples and 1.5 μmol L-1 of Na15NO3 for the deep-water triplicate samples. This amount was amended according to estimated ambient DIC concentrations of 2000 μmol L-1 and measured nitrate concentrations of 15 μmol L-1 in the deep-water. Absolute uptake rates of dissolved inorganic carbon and nitrate (ρ, DIC or NO3 taken up per unit time) were derived from the constant transport model. The 13C fraction was first calculated by dividing the measured 13C atom percentage in each sample by the natural 13C atom percentage (1.087%), over the difference between the percentage of 13C over total C in the sample and the natural 13C atom percentage. The 13C biomass was then derived by multiplying the 13C fraction by the calculated POC concentration for each sample, and 13C absolute uptake rate was measured as the 13C biomass over the incubation time (6 hours). The same calculations were applied to assess the 15N absolute uptake rates, but with the respective natural atom percentage of 15N (0.367%). Biomass-normalized uptake rates (V, DIC or NO3 taken up per unit POC or PON, respectively, per unit time) were derived from the specific uptake model, and was assessed by dividing the absolute uptake rates of DIC or NO3 by the measured POC and PON concentrations (μmol L-1), respectively. Correction for ammonium regeneration during the incubation period was not performed.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1751805 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1751805 completed Adrian Marchetti University of North Carolina at Chapel Hill 919-843-3473 123 South Rd. Chapel Hill NC 27514 USA amarchet@email.unc.edu pointOfContact Matthew P. Hurst Cal Poly Humboldt (formerly Humboldt State University) 707-826-5720 Science A 470, 1 Harpst St. Arcata CA USA matthew.hurst@humboldt.edu pointOfContact YuanYu Lin University of North Carolina at Chapel Hill 3114 Murray Hall Chapel Hill NC 27599 USA johnsony@live.unc.edu pointOfContact Claire P. Till Cal Poly Humboldt (formerly Humboldt State University) 707-826-3266 Chemistry Department 1 Harpst Street Arcata CA 95521 USA claire.till@humboldt.edu pointOfContact asNeeded Dataset Version: 1 Unknown Experiment Time_Point Hours Treatment Replicate Sample_Name Date Latitude Longitude NO3 PO4 SiOH4 chl_a_gt_5um chl_a_GFF chl_a_Total Fv_Fm Sigma PON_gt_5um PON_GFF PON_Total POC_gt_5um POC_GFF POC_Total N_Uptake_gt_5um N_Uptake_GFF N_Uptake_Total Biomass_normalized_N_Uptake_gt_5um Biomass_normalized_N_Uptake_GFF Biomass_normalized_N_Uptake_Total C_Uptake_5um C_Uptake_GFF C_Uptake_Total Biomass_normalized_C_Uptake_gt_5um Biomass_normalized_C_Uptake_GFF Biomass_normalized_C_Uptake_Total Particulate_DA Dissolved_DA Dissolved_Y Dissolved_Cd Dissolved_La Dissolved_Pb Dissolved_Ce Dissolved_Sc Dissolved_Mn Dissolved_Co Dissolved_Ni Dissolved_Cu Dissolved_Zn Dissolved_Fe Particulate_Fe_pFe_leached_gt_5um Fe_N_ratio Fe_uptake_lt_5um Fe_uptake_gt_5um Fe_uptake_lt_5um_pmol_Fe_ug_Chla_h Fe_uptake_gt_5um_pmol_Fe_ug_Chla_h Fe_uptake_lt_5um_pmol_Fe_umol_POC_h Fe_uptake_gt_5um_pmol_Fe_umol_POC_h High Resolution Inductively Coupled Plasma Mass Spectrometer isotope ratio mass spectrometer (EA-IRMS) OI Analytical Flow Solutions IV auto analyzer 10-AU fluorometers (Turner Designs) theme None, User defined experiment id time_point treatment replicate sample identification date latitude longitude Nitrate reactive phosphorus (PO4) Silicate, Si(OH)4, silicic acid chlorophyll a Fv to Fm ratio area particulate organic nitrogen particulate organic Carbon (POC) rate_measurements domoic acid trace metal concentration Cadmium Lanthanum (La) Lead Scandium Manganese Cobalt Nickel Copper Zinc Iron ratio featureType BCO-DMO Standard Parameters Inductively Coupled Plasma Mass Spectrometer Isotope-ratio Mass Spectrometer Nutrient Autoanalyzer Satlantic Submersible Ultraviolet Nitrate Analyser Turner Designs Fluorometer-10 instrument BCO-DMO Standard Instruments OC1905B service Deployment Activity California Current System place Locations otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. CAREER: An integrated molecular and physiological approach to examining the dynamics of upwelled phytoplankton in current and changing oceans https://osprey.bco-dmo.org/project/768006 CAREER: An integrated molecular and physiological approach to examining the dynamics of upwelled phytoplankton in current and changing oceans <p><em>NSF Award Abstract:</em><br /> Upwelling zones are hotspots of photosynthesis that are very dynamic in space and time. Microsocopic algae, known as phytoplankton, bloom when deep, nutrient-rich waters are upwelled into sunlit surface layers of the ocean, providing nourishment that supports productive food webs and draws down carbon dioxide (CO2) from the atmosphere to the deep ocean. Photosynthetic microbes in these regions must constantly adapt to changes in their chemical and physical environments. For example, subsurface populations respond to changes in light as they approach the surface. When upwelled waters move offshore, cells sink out of the illuminated zone, establishing seed populations that remain inactive until the next upwelling event. This process is called the upwelling conveyor belt cycle (UCBC). How phytoplankton respond to these changes in environmental conditions and how they may influence their nutrient requirements remains unknown. With future ocean changes predicted to alter seawater chemistry, including ocean acidification and decreased iron availability, some phytoplankton groups may be more vulnerable than others. Accompanying educational activities provide learning experiences to enhance understanding and awareness of marine microbes. The development of a research hub at UNC aims to provide infrastructure and support for scientists and students conducting research on environmental genomics. A laboratory component for an upper-level undergraduate course focused on marine phytoplankton is being developed. Educational outreach activities to broader communities include creation of a lesson plan on phytoplankton in upwelling zones and a virtual research cruise experience for middle-school students, as well as a hands-on lab activity for a local museum focused on marine phytoplankton and the important roles they play in shaping our planet.</p> <p>The project examines how phytoplankton respond at the molecular and physiological level to the different UCBC stages, which seed populations (i.e., surface versus subsurface) contribute most to phytoplankton blooms during upwelling events of varying intensity, how phytoplankton elemental compositions are altered throughout UCBC stages, and how future predicted ocean conditions will affect the phytoplankton responses to UCBC conditions. This project contains both laboratory and fieldwork. In the laboratory, phytoplankton isolates recently obtained from upwelling regions are exposed to simulated UCBC conditions to examine changes in gene expression, growth and photosynthetic characteristics and elemental composition. Cultures are subjected to both current and future ocean conditions, including reduced iron availability and higher CO2. In the field, research cruises within upwelling regions study the dynamics of natural phytoplankton communities (both surface and subsurface) experiencing upwelling and relaxation and within simulated upwelling incubation experiments. Knowledge of how phytoplankton are affected by UCBC conditions at an integrated molecular, physiological and elemental level under both current and future scenarios is imperative for the proper conservation and management of these critically important ecosystems.</p> Upwelled Phytoplankton Dynamics largerWorkCitation project eng; USA oceans California Current System -124.4163 -121.5429 35.92353 41.01471 2019-05-27 2019-06-04 California Upwelling Zone 0 BCO-DMO catalogue of parameters from Incubation experiments to assess the physiological and molecular response of subsurface phytoplankton communities to a simulation of the Upwelling Conveyor Belt Cycle (UCBC) conducted in summer 2019 onboard the R/V Oceanus (OC1905b) Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/953931.rdf Name: Experiment Units: unitless Description: <p>Name of experiment: Wide shelf_Fe status, Wide shelf_seed populations, Narrow shelf_Fe status, Narrow shelf_seed populations.</p> http://lod.bco-dmo.org/id/dataset-parameter/953932.rdf Name: Time_Point Units: unitless Description: <p>Time point; how long the particular sample incubated for. For Fe status incubations - T0: initial conditions before the incubation; T1: 48 hours after incubation; T2: 120 hours after incubation for wide shelf, 96 hours after incubation for narrow shelf. For seed population incubations - T0: initial conditions in the surface water SW before incubation; T1: 96 hours after incubation for wide shelf, 72 hours after incubation for narrow shelf.</p> http://lod.bco-dmo.org/id/dataset-parameter/953933.rdf Name: Hours Units: hours Description: <p>Time point in hours; how long the particular sample incubated for.</p> http://lod.bco-dmo.org/id/dataset-parameter/953934.rdf Name: Treatment Units: unitless Description: <p>Description of the treatment (in triplicates) for the particular sample. DW: initial deep water/subsurface water; Ctrl: no amendments added to incubation; Fe: 5 nM FeCl2 amended; DFB: 200 nM desferrioxamine B (DFB) amended; SW: initial surface water; Filtered DW/SW - half filtered subsurface water mixed with half unfiltered surface water; Filtered SW/DW - half filtered surface water mixed with half unfiltered subsurface water; SWDW - unfiltered surface water mixed with unfiltered subsurface water.</p> http://lod.bco-dmo.org/id/dataset-parameter/953935.rdf Name: Replicate Units: unitless Description: <p>There were three replicates for each treatment. This parameter serves to disambiguate them.</p> http://lod.bco-dmo.org/id/dataset-parameter/953936.rdf Name: Sample_Name Units: unitless Description: <p>Sample ID (marked as Q numbers) used to represent each sample.</p> http://lod.bco-dmo.org/id/dataset-parameter/953937.rdf Name: Date Units: unitless Description: <p>Date of sample collection.</p> http://lod.bco-dmo.org/id/dataset-parameter/953938.rdf Name: Latitude Units: decimal degrees Description: <p>Latitudinal coordinates of the sample site. (North = positive values).</p> http://lod.bco-dmo.org/id/dataset-parameter/953939.rdf Name: Longitude Units: decimal degrees Description: <p>Longitudinal coordinates of the sample site. (West = negative values).</p> http://lod.bco-dmo.org/id/dataset-parameter/953940.rdf Name: NO3 Units: micromolar (uM) Description: <p>Nitrate concentration.</p> http://lod.bco-dmo.org/id/dataset-parameter/953941.rdf Name: PO4 Units: micromolar (uM) Description: <p>Phosphate concentration.</p> http://lod.bco-dmo.org/id/dataset-parameter/953942.rdf Name: SiOH4 Units: micromolar (uM) Description: <p>Silicate concentration.</p> http://lod.bco-dmo.org/id/dataset-parameter/953943.rdf Name: chl_a_gt_5um Units: micrograms per liter (ug/L) Description: <p>Chl a &gt;5um: chlorophyll concentration for the greater than 5 micrometers size fraction.</p> http://lod.bco-dmo.org/id/dataset-parameter/953944.rdf Name: chl_a_GFF Units: micrograms per liter (ug/L) Description: <p>Chl a GFF [&gt;0.7um]: chlorophyll concentration for the greater than 0.7 micrometers size fraction.</p> http://lod.bco-dmo.org/id/dataset-parameter/953945.rdf Name: chl_a_Total Units: micrograms per liter (ug/L) Description: <p>Chl a Total: chlorophyll concentrations for both cell size-fractions.</p> http://lod.bco-dmo.org/id/dataset-parameter/953946.rdf Name: Fv_Fm Units: unitless Description: <p>Fv/Fm: photosynthetic efficiency measured for each sample.</p> http://lod.bco-dmo.org/id/dataset-parameter/953947.rdf Name: Sigma Units: unitless Description: <p>Sigma: Photosystem II cross-sectional area.</p> http://lod.bco-dmo.org/id/dataset-parameter/953948.rdf Name: PON_gt_5um Units: micromoles per liter (umol/L) Description: <p>PON &gt;5um: particulate organic nitrogen concentration for the greater than 5 micrometers size fraction.</p> http://lod.bco-dmo.org/id/dataset-parameter/953949.rdf Name: PON_GFF Units: micromoles per liter (umol/L) Description: <p>PON GFF [&gt;0.7um]: particulate organic nitrogen concentration for the greater than 0.7 micrometers size fraction.</p> http://lod.bco-dmo.org/id/dataset-parameter/953950.rdf Name: PON_Total Units: micromoles per liter (umol/L) Description: <p>PON Total: particulate organic nitrogen concentrations for both cell size-fractions.</p> http://lod.bco-dmo.org/id/dataset-parameter/953951.rdf Name: POC_gt_5um Units: micromoles per liter (umol/L) Description: <p>POC &gt;5um: particulate organic carbon concentration for the greater than 5 micrometers size fraction.</p> http://lod.bco-dmo.org/id/dataset-parameter/953952.rdf Name: POC_GFF Units: micromoles per liter (umol/L) Description: <p>POC GFF [&gt;0.7um]: particulate organic carbon concentration for the greater than 0.7 micrometers size fraction.</p> http://lod.bco-dmo.org/id/dataset-parameter/953953.rdf Name: POC_Total Units: micromoles per liter (umol/L) Description: <p>POC Total: particulate organic carbon concentrations for both cell size-fractions.</p> http://lod.bco-dmo.org/id/dataset-parameter/953954.rdf Name: N_Uptake_gt_5um Units: micromoles per liter per day (umol/L/day) Description: <p>N Uptake &gt;5um: absolute nitrate uptake rates for the greater than 5 micrometers size fraction.</p> http://lod.bco-dmo.org/id/dataset-parameter/953955.rdf Name: N_Uptake_GFF Units: micromoles per liter per day (umol/L/day) Description: <p>N Uptake GFF: absolute nitrate uptake rates for the greater than 0.7 micrometers size fraction.</p> http://lod.bco-dmo.org/id/dataset-parameter/953956.rdf Name: N_Uptake_Total Units: micromoles per liter per day (umol/L/day) Description: <p>N Uptake Total: absolute nitrate uptake rates for both cell size-fractions.</p> http://lod.bco-dmo.org/id/dataset-parameter/953957.rdf Name: Biomass_normalized_N_Uptake_gt_5um Units: per day Description: <p>Biomass-normalized N Uptake &gt;5um: biomass-normalized nitrate uptake rates for the greater than 5 micrometers size fraction.</p> http://lod.bco-dmo.org/id/dataset-parameter/953958.rdf Name: Biomass_normalized_N_Uptake_GFF Units: per day Description: <p>Biomass-normalized N Uptake GFF: biomass-normalized nitrate uptake rates for the greater than 0..7 micrometers size fraction.</p> http://lod.bco-dmo.org/id/dataset-parameter/953959.rdf Name: Biomass_normalized_N_Uptake_Total Units: per day Description: <p>Biomass-normalized N Uptake Total: biomass-normalized nitrate uptake rates for both cell size-fractions.</p> http://lod.bco-dmo.org/id/dataset-parameter/953960.rdf Name: C_Uptake_5um Units: micromoles per liter per day (umol/L/day) Description: <p>C Uptake &gt;5um: absolute dissolved inorganic carbon (DIC) uptake rates for the greater than 5 micrometers size fraction.</p> http://lod.bco-dmo.org/id/dataset-parameter/953961.rdf Name: C_Uptake_GFF Units: micromoles per liter per day (umol/L/day) Description: <p>C Uptake GFF: absolute dissolved inorganic carbon (DIC) uptake rates for the greater than 0..7 micrometers size fraction.</p> http://lod.bco-dmo.org/id/dataset-parameter/953962.rdf Name: C_Uptake_Total Units: micromoles per liter per day (umol/L/day) Description: <p>C Uptake Total: absolute dissolved inorganic carbon (DIC) uptake rates for both cell size-fractions.</p> http://lod.bco-dmo.org/id/dataset-parameter/953963.rdf Name: Biomass_normalized_C_Uptake_gt_5um Units: per day Description: <p>Biomass-normalized C Uptake &gt;5um: biomass-normalized dissolved inorganic carbon (DIC) uptake rates for the greater than 5 micrometers size fraction.</p> http://lod.bco-dmo.org/id/dataset-parameter/953964.rdf Name: Biomass_normalized_C_Uptake_GFF Units: per day Description: <p>Biomass-normalized C Uptake GFF: biomass-normalized dissolved inorganic carbon (DIC) uptake rates for the greater than 0..7 micrometers size fraction.</p> http://lod.bco-dmo.org/id/dataset-parameter/953965.rdf Name: Biomass_normalized_C_Uptake_Total Units: per day Description: <p>Biomass-normalized C Uptake Total: biomass-normalized dissolved inorganic carbon (DIC) uptake rates for both cell size-fractions.</p> http://lod.bco-dmo.org/id/dataset-parameter/953966.rdf Name: Particulate_DA Units: micrograms per liter (ug/L) Description: <p>Particulate DA: domoic acid concentrations in the particulate fraction.</p> http://lod.bco-dmo.org/id/dataset-parameter/953967.rdf Name: Dissolved_DA Units: micrograms per liter (ug/L) Description: <p>Dissolved DA: domoic acid concentrations in the dissolved fraction.</p> http://lod.bco-dmo.org/id/dataset-parameter/953968.rdf Name: Dissolved_Y Units: picomoles per kilogram (pmol/kg) Description: <p>Dissolved yttrium concentration.</p> http://lod.bco-dmo.org/id/dataset-parameter/953969.rdf Name: Dissolved_Cd Units: picomoles per kilogram (pmol/kg) Description: <p>Dissolved cadmium concentration.</p> http://lod.bco-dmo.org/id/dataset-parameter/953970.rdf Name: Dissolved_La Units: picomoles per kilogram (pmol/kg) Description: <p>Dissolved lanthanum concentration.</p> http://lod.bco-dmo.org/id/dataset-parameter/953971.rdf Name: Dissolved_Pb Units: picomoles per kilogram (pmol/kg) Description: <p>Dissolved lead concentration.</p> http://lod.bco-dmo.org/id/dataset-parameter/953972.rdf Name: Dissolved_Ce Units: picomoles per kilogram (pmol/kg) Description: <p>Dissolved cerium concentration.</p> http://lod.bco-dmo.org/id/dataset-parameter/953973.rdf Name: Dissolved_Sc Units: picomoles per kilogram (pmol/kg) Description: <p>Dissolved scandium concentration.</p> http://lod.bco-dmo.org/id/dataset-parameter/953974.rdf Name: Dissolved_Mn Units: nanomoles per kilogram (nmol/kg) Description: <p>Dissolved manganese concentration.</p> http://lod.bco-dmo.org/id/dataset-parameter/953975.rdf Name: Dissolved_Co Units: picomoles per kilogram (pmol/kg) Description: <p>Dissolved cobalt concentration.</p> http://lod.bco-dmo.org/id/dataset-parameter/953976.rdf Name: Dissolved_Ni Units: nanomoles per kilogram (nmol/kg) Description: <p>Dissolved nickel concentration.</p> http://lod.bco-dmo.org/id/dataset-parameter/953977.rdf Name: Dissolved_Cu Units: nanomoles per kilogram (nmol/kg) Description: <p>Dissolved copper concentration.</p> http://lod.bco-dmo.org/id/dataset-parameter/953978.rdf Name: Dissolved_Zn Units: nanomoles per kilogram (nmol/kg) Description: <p>Dissolved zinc concentration.</p> http://lod.bco-dmo.org/id/dataset-parameter/953979.rdf Name: Dissolved_Fe Units: nanomoles per kilogram (nmol/kg) Description: <p>Dissolved iron concentration.</p> http://lod.bco-dmo.org/id/dataset-parameter/953980.rdf Name: Particulate_Fe_pFe_leached_gt_5um Units: nanomoles per kilogram (nmol/kg) Description: <p>Particulate iron concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/953981.rdf Name: Fe_N_ratio Units: unitless Description: <p>Fe/N ratio</p> http://lod.bco-dmo.org/id/dataset-parameter/953982.rdf Name: Fe_uptake_lt_5um Units: picomoles Fe per liter per hour (pmol Fe/L/h) Description: <p>Fe uptake (&lt;5um)</p> http://lod.bco-dmo.org/id/dataset-parameter/953983.rdf Name: Fe_uptake_gt_5um Units: picomoles Fe per liter per hour (pmol Fe/L/h) Description: <p>Fe uptake (&gt;5um)</p> http://lod.bco-dmo.org/id/dataset-parameter/953984.rdf Name: Fe_uptake_lt_5um_pmol_Fe_ug_Chla_h Units: picomoles Fe per microgram chla per hour (pmol Fe/ug Chla/h) Description: <p>Fe uptake (&lt;5um)</p> http://lod.bco-dmo.org/id/dataset-parameter/953985.rdf Name: Fe_uptake_gt_5um_pmol_Fe_ug_Chla_h Units: picomoles Fe per microgram chla per hour (pmol Fe/ug Chla/h) Description: <p>Fe uptake (&gt;5um)</p> http://lod.bco-dmo.org/id/dataset-parameter/953986.rdf Name: Fe_uptake_lt_5um_pmol_Fe_umol_POC_h Units: picomoles Fe per micromole POC per hour (pmol Fe/umol POC/h) Description: <p>Fe uptake (&lt;5um)</p> http://lod.bco-dmo.org/id/dataset-parameter/953987.rdf Name: Fe_uptake_gt_5um_pmol_Fe_umol_POC_h Units: picomoles Fe per micromole POC per hour (pmol Fe/umol POC/h) Description: <p>Fe uptake (&gt;5um)</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 35520 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/b6a0f3f5-0d8d-4c5e-ae94-03a4a358d64d/content download https://doi.org/10.26008/1912/bco-dmo.949788.1 download onLine dataset &lt;p&gt;&lt;strong&gt;For the Fe status incubations:&lt;/strong&gt;&lt;br /&gt; Seawater was collected using trace-metal clean techniques from a depth of 90 meters (m) (corresponding to the 8.4 degree Celsius isotherm) for the wide shelf incubation and 80 m (corresponding to the 8.9 degree Celsius isotherm) for the narrow shelf incubation. Seawater from both sites was pumped directly into a positive pressure trace-metal clean plastic bubble created in the ship’s laboratory into large, 50-gallon acid-washed high-density polyethylene (HDPE) drums to homogenize the seawater using a Wilden air-operated double-diaphragm pump made of polytetrafluoroethylene and acid-washed HDPE tubing. Preparation of the cubitainers was carried out prior to the cruise and included trace-metal clean techniques. Cubitainers were initially soaked in 2% Extran detergent for 7 days, then rinsed with deionized water four times and Milli-Q water three times prior to being soaked in 10% reagent grade hydrochloric acid for 2-3 days and rinsed with Milli-Q water. Subsequently, the cubitainers were soaked in 1% trace metal grade hydrochloric acid for 7 days and rinsed with Milli-Q water, then soaked in 0.1% trace metal grade acetic acid 3-4 days before final storage in low Fe water from Station P (50 degrees North,145 degrees West). Station P water had been collected and filtered from the 2018 EXPORTS North Pacific field campaign using trace metal clean techniques and stored in the dark at 4 degrees Celsius (°C) until use. In the bubble, these triplicate acid-cleaned 10-liter (L) low-density polyethylene cubitainers were filled and then incubated in large on-deck plexiglass incubators circulated with water chilled to the temperatures at which the subsurface samples were collected using Aqua Logic Delta Star® In-Line Water Chillers. Incubators were covered with neutral density screening to achieve 30% incident irradiance. To assess the effects of Fe addition or removal on the simulated upwelled plankton communities, samples were incubated with no amendment (control), amended with 5 nanomolar (nM) of FeCl2 (Fe treatment), or amended with 200 nM desferrioxamine B (DFB), a strong Fe chelator, which inhibits dissolved Fe uptake (DFB treatment). Three cubitainers were immediately harvested for the initial time- point (T0), also referred to as deep-water or DW. The remaining cubitainers were incubated for two additional time points for a total of 18 cubitainers per incubation. Cubitainers were harvested for various biological and chemical parameters (see below) following 48 hours (T1) for both incubation experiments, and following 120 hours in the wide shelf incubation and 96 hours in the narrow shelf incubation (T2).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;For the seed population incubations:&lt;/strong&gt;&lt;br /&gt; Seawater was collected using trace-metal clean techniques at 15 m depth for the surface incubations and at 90 m for the subsurface incubations (corresponding to the 8.4 °C isotherm). Seawater was pumped directly into a positive pressure trace metal clean plastic bubble created in the ship's laboratory into large, 50-gallon acid-washed high-density polyethylene (HDPE) drums to homogenize the seawater using a Wilden air-operated double-diaphragm pump made of polytetrafluoroethylene (PTFE) and acid-washed HDPE tubing. Preparation of the cubitainers was carried out prior to the cruise and included trace-metal clean techniques (Crawford et al., 2003): 10 L cubitainers were initially soaked in 2% Extran detergent for 7 days, then rinsed with deionized water four times and Milli-Q water three times prior to being soaked in 10% reagent grade hydrochloric acid for 2-3 days and rinsed with Milli-Q water. Subsequently, the cubitainers were soaked in 1% trace metal grade hydrochloric acid for 7 days and rinsed with Milli-Q water, then soaked in 0.1% trace metal grade acetic acid 3-4 days before final storage in low-Fe water from Ocean Station Papa (St. P). The St. P water had been collected and filtered from the 2018 EXPORTS North Pacific (50 °N, 145 °W) field campaign using trace metal clean techniques and stored in the dark at 4 °C until use. In the bubble, these triplicate acid-cleaned 10 L low-density polyethylene (LDPE) cubitainers were filled and then incubated in large on-deck plexiglass incubators circulated with water chilled to the temperatures at which the subsurface samples were collected using Aqua Logic Delta Star® In-Line Water Chillers. Incubators were covered with neutral density screening to achieve 30% incident irradiance. For the experimental design, a portion of the surface and subsurface collections was directly collected and denoted as the initial (T0) surface and subsurface communities, respectively. The resulting incubations (triplicates) were characterized by either the surface waters (SW) or the subsurface waters (DW) incubated for 96 hours (T1). Another portion (triplicates) was subjected to mixture with filtered waters from the respective subsurface or surface communities, such that the 96 hour incubations consisted of an equal mixture of filtered-subsurface waters and surface waters, denoted as FDW/SW (filtered deep water/surface water), an equal mixture of filtered-surface waters and subsurface waters, denoted as FSW/DW (filtered surface water/deep water), and an equal mixture of both unfiltered surface and subsurface waters, denoted as mixed (surface water/deep water). No additional macronutrients or micronutrients were added to any of the incubations. Cubitainers were harvested for various biological and chemical parameters (see below) in the initial time point and following 96 hours of incubation.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Physiological Measurements:&lt;/strong&gt;&lt;br /&gt; For chlorophyll a measurements, 250 milliliters (mL) of seawater was gravity filtered through 5-micrometer (μm) Isopore membrane filters (47-millimeters (mm)) and subsequently vacuum filtered onto GF/F (25 mm) filters under 100 mmHg of vacuum pressure. Filters were then rinsed with 0.45 μm filtered seawater and immediately stored at -20 °C until onshore analysis in the lab. Chlorophyll a extraction was performed using a 90% acetone solution at -20 °C for 24 hours and measured on a 10-AU fluorometer (Turner Designs, San Jose, CA) using the acidification method.&lt;/p&gt; &lt;p&gt;Dissolved inorganic nutrients (nitrate + nitrite, phosphate, and silicic acid) were measured by filtering 30 mL of water through a 0.2 µm filter, using acid-washed syringes into an acid-cleaned polypropylene FalconTM tube. Dissolved nutrient concentrations were analyzed using an OI Analytical Flow Solutions IV auto analyzer by Wetland Biogeochemistry Analytical Services at Louisiana State University. Concentrations of nitrate measured from the discrete samples were used for the calculation of absolute nitrate uptake rates.&lt;/p&gt; &lt;p&gt;Samples for dissolved iron (dFe) were collected from each cubitainer within a trace-metal clean, positive pressure plastic bubble by filtering through pre-cleaned 0.2 μm pore size polyethersulfone membrane Acropak-200® capsule filters into LDPE bottles that had been rigorously cleaned as described in the GEOTRACES cookbook. Sample bottles were rinsed three times with sample before filling. Samples were acidified at sea to pH ~1.7 with optima HCl (2 mL of 12 M HCl per liter of seawater) and were analyzed after the cruise. Briefly, this method involves pre-concentration onto Nobias-chelate PA1 resin followed by analysis with a High-Resolution Inductively Coupled Plasma Mass Spectrometer. For quality control, a few samples were rerun with a flow injection analysis method. This method involves pre-concentration on toyopearl resin followed by in-line spectrophotometric analysis.&lt;/p&gt; &lt;p&gt;To assess the isotope uptake of DIC and NO3, a 618 mL polycarbonate bottle was filled to the top with incubation samples from each cubitainer. The subsamples were collected for dissolved inorganic carbon (DIC) and nitrate uptake rates at each respective time point, and immediately spiked with both NaH13CO3 and Na15NO3 at approximately 10% of the estimated ambient DIC concentrations and measured nitrate concentrations in a trace metal clean (TMC) space located on the ship (see below). Since there were no underway measurements of DIC, an expected ambient concentration of 2000 micromoles per liter (μmol L-1) was derived from literature values for both incubation sites. Nitrate concentrations of the deep-water and surface water in both incubation sites were measured using the Submersible Ultraviolet Nitrate Analyzer (SUNA), which quantifies dissolved nitrate concentrations by illuminating the water sample with UV and using the absorbance values to estimate nitrate concentrations from a multi-variable linear regression based on the MBARI method. After spiking with respective trace concentrations of both stable isotopes of DIC and nitrate, samples were returned to the incubator for six hours. All rate measurement incubations were initiated at approximately the same time of day (i.e., close to dawn). Following incubation, seawater was immediately filtered where cells greater than 5 μm were gravity filtered onto 5 μm Isopore membrane filters (47 mm) and then washed onto a pre-combusted (450 °C for 5 hours) GF/F filters (0.7 μm nominal porosity) by vacuum filtration using 0.2 μm filtered seawater and preserved at -20 °C. Cells smaller than 5 μm passed through the initial 5 μm Isopore membrane filters and were collected on pre-combusted GF/F filters and preserved at -20 °C. Prior to analysis, filters were dried at 60 °C for 24 hours, encapsulated in tin and pelletized. Particulate organic carbon (POC), particulate organic nitrogen (PON), and atom percentages of 13C and 15N were subsequently quantified using an isotope ratio mass spectrometer (EA-IRMS) at the UC Davis Stable Isotope Facility. For each sample, POC and PON concentrations (μmol L-1) were calculated by dividing the measured POC/PON mass (micrograms (μg)) by the respective atomic mass of carbon and nitrogen over the volume filtered (0.62 L).&lt;/p&gt; &lt;p&gt;For the wide shelf incubation, samples were spiked with 200 μmol L-1 of NaH13CO3 and 1 μmol L-1 of Na15NO3 according to estimated ambient DIC concentrations of 2000 μmol L-1 and measured surface nitrate concentrations of 10 μmol L-1. For the narrow shelf incubation, samples were spiked with 200 μmol L-1 of NaH13CO3 for all samples and 1.5 μmol L-1 of Na15NO3 for the deep-water triplicate samples. This amount was amended according to estimated ambient DIC concentrations of 2000 μmol L-1 and measured nitrate concentrations of 15 μmol L-1 in the deep-water. Absolute uptake rates of dissolved inorganic carbon and nitrate (ρ, DIC or NO3 taken up per unit time) were derived from the constant transport model. The 13C fraction was first calculated by dividing the measured 13C atom percentage in each sample by the natural 13C atom percentage (1.087%), over the difference between the percentage of 13C over total C in the sample and the natural 13C atom percentage. The 13C biomass was then derived by multiplying the 13C fraction by the calculated POC concentration for each sample, and 13C absolute uptake rate was measured as the 13C biomass over the incubation time (6 hours). The same calculations were applied to assess the 15N absolute uptake rates, but with the respective natural atom percentage of 15N (0.367%). Biomass-normalized uptake rates (V, DIC or NO3 taken up per unit POC or PON, respectively, per unit time) were derived from the specific uptake model, and was assessed by dividing the absolute uptake rates of DIC or NO3 by the measured POC and PON concentrations (μmol L-1), respectively. Correction for ammonium regeneration during the incubation period was not performed.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;GraphPad Prism was used to plot both the environmental and physiological data.&lt;/p&gt; Specified by the Principal Investigator(s) - Imported original file &quot;PUPCYCLE_compiled_results_Q_experiments.csv&quot; into the BCO-DMO system. - Renamed fields to comply with BCO-DMO naming conventions. - Converted dates to YYYY-MM-DD format. - Saved the final file as &quot;949788_v1_pupcycle_2019_incubation_experiments.csv&quot;. - This dataset has been published &quot;as is&quot;. Final review by the data submitter was not received after the data were imported into the BCO-DMO data system. Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact High Resolution Inductively Coupled Plasma Mass Spectrometer High Resolution Inductively Coupled Plasma Mass Spectrometer PI Supplied Instrument Name: High Resolution Inductively Coupled Plasma Mass Spectrometer PI Supplied Instrument Description:Used in analysis of samples for dissolved iron (dFe). Instrument Name: Inductively Coupled Plasma Mass Spectrometer Instrument Short Name:ICP Mass Spec Instrument Description: An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB15/ isotope ratio mass spectrometer (EA-IRMS) isotope ratio mass spectrometer (EA-IRMS) PI Supplied Instrument Name: isotope ratio mass spectrometer (EA-IRMS) PI Supplied Instrument Description:Atom percentages of 13C and 15N were quantified using an isotope ratio mass spectrometer (EA-IRMS) at the UC Davis Stable Isotope Facility. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/ OI Analytical Flow Solutions IV auto analyzer OI Analytical Flow Solutions IV auto analyzer PI Supplied Instrument Name: OI Analytical Flow Solutions IV auto analyzer PI Supplied Instrument Description:Dissolved inorganic nutrients were measured using the OI Analytical Flow Solutions IV auto analyzer by Wetland Biogeochemistry Analytical Services at Louisiana State University. Instrument Name: Nutrient Autoanalyzer Instrument Short Name:Nutrient Autoanalyzer Instrument Description: Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/ PI Supplied Instrument Name: PI Supplied Instrument Description:Nitrate concentrations of the deep-water and surface water in both incubation sites were measured using the Submersible Ultraviolet Nitrate Analyzer (SUNA). Instrument Name: Satlantic Submersible Ultraviolet Nitrate Analyser Instrument Short Name:SUNA Instrument Description: The Submersible Ultraviolet Nitrate Analyser (SUNA) uses ultraviolet absorption spectroscopy to measure in-situ dissolved nitrate. It is a chemical-free method that allows real-time and continuous nitrate concentration measurements in a variety of environments. It has a 1 cm path length, 190 - 370 nm wavelength range and a depth rating of 100 m. 10-AU fluorometers (Turner Designs) 10-AU fluorometers (Turner Designs) PI Supplied Instrument Name: 10-AU fluorometers (Turner Designs) PI Supplied Instrument Description:Chlorophyll was measured using a 10-AU fluorometers (Turner Designs, San Jose, CA). Instrument Name: Turner Designs Fluorometer-10 Instrument Short Name:Turner Fluorometer-10 Instrument Description: The Turner Designs Model 10 fluorometer (manufactured by Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA) is used to measure Chlorophyll fluorescence. No information could be found for this specific model. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/ Cruise: OC1905B OC1905B R/V Oceanus Community Standard Description International Council for the Exploration of the Sea R/V Oceanus vessel OC1905B Adrian Marchetti R/V Oceanus Community Standard Description International Council for the Exploration of the Sea R/V Oceanus vessel