http://lod.bco-dmo.org/id/dataset/949801
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-01-28
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Data from samples collected at four sites experiencing upwelling off the coast of Northern California in May and June of 2019
2025-02-20
publication
2025-02-20
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-08-07
publication
https://doi.org/10.26008/1912/bco-dmo.949801.1
Adrian Marchetti
University of North Carolina at Chapel Hill
principalInvestigator
Matthew P. Hurst
Cal Poly Humboldt (formerly Humboldt State University)
principalInvestigator
YuanYu Lin
University of North Carolina at Chapel Hill
principalInvestigator
Claire P. Till
Cal Poly Humboldt (formerly Humboldt State University)
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lin, Y., Marchetti, A., Till, C. P., Hurst, M. P. (2025) Data from samples collected at four sites experiencing upwelling off the coast of Northern California in May and June of 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-02-20 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.949801.1 [access date]
PUPCYCLE 2019 upwelling transects Dataset Description: Methods and Sampling: <p><strong>Sample collection:</strong><br />
Four sites experiencing upwelling (E1: 41° 57.152 latitude, 124° 25.005 longitude. L2: 41° 42.078 latitude, 124° 30.865 longitude. L3: 43° 33.183 latitude, 124° 22.312 longitude. L4: 43° 59.988 latitude, 124° 41.28 longitude) were sampled off the coast of Northern California on May 30, 2019 (E1 and L2) and June 05, 2019 (L3 and L4) aboard the R/V Oceanus. Seawater was collected using a CTD-Rosette sampler, at four depths throughout the euphotic zone at each station corresponding to 50%, 30%, 10% and 1% incident irradiance (E1: 5.7 meters (m), 8.5m, 14.5m, 26.9m. L2: 5.7m, 9m, 16m, 27m. L3 and L4: 5m, 7m, 14m, 27m). At each depth, samples for chlorophyll a (Chl a), dissolved inorganic nutrients, dissolved inorganic carbon and nitrate uptake rates, cell counts and gene expression were obtained.</p>
<p><strong>Physiological Measurements:</strong><br />
For chlorophyll a measurements, 250 milliliters (mL) of seawater was gravity filtered through 5 micrometer (μm) Isopore membrane filters (47 millimeters (mm)) and subsequently vacuum filtered onto GF/F (25 mm) filters under 100 mmHg of vacuum pressure. Filters were then rinsed with 0.45 μm filtered seawater and immediately stored at -20 degrees Celsius (°C) until onshore analysis in the lab. Chlorophyll a extraction was performed using a 90% acetone solution at -20 °C for 24 hours and measured on a 10-AU fluorometer (Turner Designs, San Jose, CA) using the acidification method.</p>
<p>Dissolved inorganic nutrients (nitrate + nitrite, phosphate and silicic acid) were measured by filtering 30 mL of water through a 0.2 µm filter, using acid-washed syringes into an acid-cleaned polypropylene FalconTM tube. Dissolved nutrient concentrations were analyzed using an OI Analytical Flow Solutions IV auto analyzer by Wetland Biogeochemistry Analytical Services at Louisiana State University. Concentrations of nitrate measured from the discrete samples were used for the calculation of absolute nitrate uptake rates.</p>
<p>Samples for dissolved iron (dFe) were collected from each cubitainer within a trace-metal clean, positive pressure plastic bubble by filtering through pre-cleaned 0.2 um pore size polyethersulfone membrane Acropak-200® capsule filters into LDPE bottles that had been rigorously cleaned as described in the GEOTRACES cookbook. Sample bottles were rinsed three times with sample before filling. Samples were acidified at sea to pH ~1.7 with optima HCl (2 mL of 12 M HCl per liter of seawater) and were analyzed after the cruise. Briefly, this method involves pre-concentration onto Nobias-chelate PA1 resin followed by analysis with a High Resolution Inductively Coupled Plasma Mass Spectrometer. For quality control, a few samples were rerun with a flow injection analysis method. This method involves pre-concentration on toyopearl resin followed by in-line spectrophotometric analysis.</p>
<p>Seawater from the rosette was collected into polycarbonate bottles (~618 mL) and immediately spiked with both NaH13CO3 and Na15NO3 at approximately 10% of the predicted ambient DIC concentrations and measured nitrate concentrations under a trace metal clean (TMC) flow hood located on the ship. Samples were then incubated for six hours, and vacuum filtered similarly to Chl a measurements, such that cells greater than or equal to 5 μm were collected onto 5 μm Isopore polycarbonate membrane filters (47 mm), with the flow through containing cells smaller than 5 μm which were collected onto 0.7 μm pre-combusted GF/F filters. The polycarbonate filters containing cells greater than 5 μm were then rinsed onto new 0.7 μm pre-combusted GF/F filters using 0.45 μm filtered-seawater. The filters were then preserved at -20 °C until further processing in the laboratory. Prior to analysis, filters were dried at 60 °C for 24 hours, encapsulated in tin, and pelletized. Particulate organic carbon (POC), particulate organic nitrogen (PON), and atom percentages of 13C and 15N were subsequently quantified using an isotope ratio mass spectrometer (EA-IRMS) at the UC Davis Stable Isotope Facility. For each sample, POC and PON concentrations (micromoles per liter (μmol L-1)) were calculated by dividing the measured POC/PON mass (micrograms (μg)) by the respective atomic mass of carbon and nitrogen over the volume filtered (0.5 liter (L)).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1751805 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1751805
completed
Adrian Marchetti
University of North Carolina at Chapel Hill
919-843-3473
123 South Rd.
Chapel Hill
NC
27514
USA
amarchet@email.unc.edu
pointOfContact
Matthew P. Hurst
Cal Poly Humboldt (formerly Humboldt State University)
707-826-5720
Science A 470, 1 Harpst St.
Arcata
CA
USA
matthew.hurst@humboldt.edu
pointOfContact
YuanYu Lin
University of North Carolina at Chapel Hill
3114 Murray Hall
Chapel Hill
NC
27599
USA
johnsony@live.unc.edu
pointOfContact
Claire P. Till
Cal Poly Humboldt (formerly Humboldt State University)
707-826-3266
Chemistry Department 1 Harpst Street
Arcata
CA
95521
USA
claire.till@humboldt.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Station
Replicate
Depth_m
Sample_Name
Date
Latitude
Longitude
Salinity
Temperature
PAR
NO3
PO4
SiO2
chl_a_gt_5um
chl_a_GFF
chl_a_Total
PON_gt_5um
PON_GFF
PON_Total
POC_gt_5um
POC_GFF
POC_Total
N_Uptake_gt_5um
N_Uptake_GFF
N_Uptake_Total
Biomass_normalized_N_Uptake_gt_5um
Biomass_normalized_N_Uptake_GFF
Biomass_normalized_N_Uptake_Total
C_Uptake_5um
C_Uptake_GFF
C_Uptake_Total
Biomass_normalized_C_Uptake_gt_5um
Biomass_normalized_C_Uptake_GFF
Biomass_normalized_C_Uptake_Total
dissolved_Y
dissolved_Cd
dissolved_La
dissolved_Pb
dissolved_Ce
dissolved_Sc
dissolved_Mn
dissolved_Fe
dissolved_Co
dissolved_Ni
dissolved_Cu
dissolved_Zn
soluble_Fe
soluble_Ni
soluble_Cu
soluble_Zn
CTD-Rosette sampler
High Resolution Inductively Coupled Plasma Mass Spectrometer
isotope ratio mass spectrometer (EA-IRMS)
OI Analytical Flow Solutions IV auto analyzer
10-AU fluorometers (Turner Designs)
theme
None, User defined
station
replicate
depth
sample identification
date
latitude
longitude
salinity
water temperature at measurement depth
PAR
Nitrate
reactive phosphorus (PO4)
Silicate, Si(OH)4, silicic acid
chlorophyll a
particulate organic nitrogen
particulate organic Carbon (POC)
rate_measurements
trace metal concentration
Cadmium
Lanthanum (La)
Lead
Scandium
Manganese
Iron
Cobalt
Nickel
Copper
Zinc
featureType
BCO-DMO Standard Parameters
CTD - profiler
Inductively Coupled Plasma Mass Spectrometer
Isotope-ratio Mass Spectrometer
Nutrient Autoanalyzer
Turner Designs Fluorometer 10-AU
instrument
BCO-DMO Standard Instruments
OC1905B
service
Deployment Activity
California Current System
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
CAREER: An integrated molecular and physiological approach to examining the dynamics of upwelled phytoplankton in current and changing oceans
https://osprey.bco-dmo.org/project/768006
CAREER: An integrated molecular and physiological approach to examining the dynamics of upwelled phytoplankton in current and changing oceans
<p><em>NSF Award Abstract:</em><br />
Upwelling zones are hotspots of photosynthesis that are very dynamic in space and time. Microsocopic algae, known as phytoplankton, bloom when deep, nutrient-rich waters are upwelled into sunlit surface layers of the ocean, providing nourishment that supports productive food webs and draws down carbon dioxide (CO2) from the atmosphere to the deep ocean. Photosynthetic microbes in these regions must constantly adapt to changes in their chemical and physical environments. For example, subsurface populations respond to changes in light as they approach the surface. When upwelled waters move offshore, cells sink out of the illuminated zone, establishing seed populations that remain inactive until the next upwelling event. This process is called the upwelling conveyor belt cycle (UCBC). How phytoplankton respond to these changes in environmental conditions and how they may influence their nutrient requirements remains unknown. With future ocean changes predicted to alter seawater chemistry, including ocean acidification and decreased iron availability, some phytoplankton groups may be more vulnerable than others. Accompanying educational activities provide learning experiences to enhance understanding and awareness of marine microbes. The development of a research hub at UNC aims to provide infrastructure and support for scientists and students conducting research on environmental genomics. A laboratory component for an upper-level undergraduate course focused on marine phytoplankton is being developed. Educational outreach activities to broader communities include creation of a lesson plan on phytoplankton in upwelling zones and a virtual research cruise experience for middle-school students, as well as a hands-on lab activity for a local museum focused on marine phytoplankton and the important roles they play in shaping our planet.</p>
<p>The project examines how phytoplankton respond at the molecular and physiological level to the different UCBC stages, which seed populations (i.e., surface versus subsurface) contribute most to phytoplankton blooms during upwelling events of varying intensity, how phytoplankton elemental compositions are altered throughout UCBC stages, and how future predicted ocean conditions will affect the phytoplankton responses to UCBC conditions. This project contains both laboratory and fieldwork. In the laboratory, phytoplankton isolates recently obtained from upwelling regions are exposed to simulated UCBC conditions to examine changes in gene expression, growth and photosynthetic characteristics and elemental composition. Cultures are subjected to both current and future ocean conditions, including reduced iron availability and higher CO2. In the field, research cruises within upwelling regions study the dynamics of natural phytoplankton communities (both surface and subsurface) experiencing upwelling and relaxation and within simulated upwelling incubation experiments. Knowledge of how phytoplankton are affected by UCBC conditions at an integrated molecular, physiological and elemental level under both current and future scenarios is imperative for the proper conservation and management of these critically important ecosystems.</p>
Upwelled Phytoplankton Dynamics
largerWorkCitation
project
eng; USA
oceans
California Current System
-124.6888
-124.3713
41.42078
43.99948
2019-05-30
2019-06-05
California Upwelling Zone
0
BCO-DMO catalogue of parameters from Data from samples collected at four sites experiencing upwelling off the coast of Northern California in May and June of 2019
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/954056.rdf
Name: Station
Units: unitless
Description: <p>Used to denote the four different upwelling transect stations (E1: early upwelling station; L2, L3, L4: later upwelling stations).</p>
http://lod.bco-dmo.org/id/dataset-parameter/954057.rdf
Name: Replicate
Units: unitless
Description: <p>There were three replicates for each treatment. This parameter serves to disambiguate them.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954058.rdf
Name: Depth_m
Units: meters (m)
Description: <p>Depth in meters at which each sample was collected.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954059.rdf
Name: Sample_Name
Units: unitless
Description: <p>Sample IDs (marked as UT numbers) used to represent each sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954060.rdf
Name: Date
Units: unitless
Description: <p>Date of sample collection.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954061.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitudinal coordinates of the sample site. (North = positive values).</p>
http://lod.bco-dmo.org/id/dataset-parameter/954062.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitudinal coordinates of the sample site. (West = negative values).</p>
http://lod.bco-dmo.org/id/dataset-parameter/954063.rdf
Name: Salinity
Units: PSU
Description: <p>Salinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/954064.rdf
Name: Temperature
Units: degrees Celsius
Description: <p>Water temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/954065.rdf
Name: PAR
Units: watts per square meter (W/m^2)
Description: <p>PAR</p>
http://lod.bco-dmo.org/id/dataset-parameter/954066.rdf
Name: NO3
Units: micromolar (uM)
Description: <p>Nitrate concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954067.rdf
Name: PO4
Units: micromolar (uM)
Description: <p>Phosphate concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954068.rdf
Name: SiO2
Units: micromolar (uM)
Description: <p>Silicate concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954069.rdf
Name: chl_a_gt_5um
Units: micrograms per liter (ug/L)
Description: <p>Chl a >5um: chlorophyll concentration for the greater than 5 micrometers size fraction.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954070.rdf
Name: chl_a_GFF
Units: micrograms per liter (ug/L)
Description: <p>Chl a GFF [>0.7um]: chlorophyll concentration for the greater than 0.7 micrometers size fraction.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954071.rdf
Name: chl_a_Total
Units: micrograms per liter (ug/L)
Description: <p>Chl a Total: chlorophyll concentrations for both cell size-fractions.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954072.rdf
Name: PON_gt_5um
Units: micromoles per liter (umol/L)
Description: <p>PON >5um: particulate organic nitrogen concentration for the greater than 5 micrometers size fraction.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954073.rdf
Name: PON_GFF
Units: micromoles per liter (umol/L)
Description: <p>PON GFF [>0.7um]: particulate organic nitrogen concentration for the greater than 0.7 micrometers size fraction.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954074.rdf
Name: PON_Total
Units: micromoles per liter (umol/L)
Description: <p>PON Total: particulate organic nitrogen concentrations for both cell size-fractions.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954075.rdf
Name: POC_gt_5um
Units: micromoles per liter (umol/L)
Description: <p>POC >5um: particulate organic carbon concentration for the greater than 5 micrometers size fraction.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954076.rdf
Name: POC_GFF
Units: micromoles per liter (umol/L)
Description: <p>POC GFF [>0.7um]: particulate organic carbon concentration for the greater than 0.7 micrometers size fraction.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954077.rdf
Name: POC_Total
Units: micromoles per liter (umol/L)
Description: <p>POC Total: particulate organic carbon concentrations for both cell size-fractions.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954078.rdf
Name: N_Uptake_gt_5um
Units: micromoles per liter per day (umol/L/day)
Description: <p>N Uptake >5um: absolute nitrate uptake rates for the greater than 5 micrometers size fraction.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954079.rdf
Name: N_Uptake_GFF
Units: micromoles per liter per day (umol/L/day)
Description: <p>N Uptake GFF: absolute nitrate uptake rates for the greater than 0.7 micrometers size fraction.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954080.rdf
Name: N_Uptake_Total
Units: micromoles per liter per day (umol/L/day)
Description: <p>N Uptake Total: absolute nitrate uptake rates for both cell size-fractions.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954081.rdf
Name: Biomass_normalized_N_Uptake_gt_5um
Units: per day
Description: <p>Biomass-normalized N Uptake >5um: biomass-normalized nitrate uptake rates for the greater than 5 micrometers size fraction.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954082.rdf
Name: Biomass_normalized_N_Uptake_GFF
Units: per day
Description: <p>Biomass-normalized N Uptake GFF: biomass-normalized nitrate uptake rates for the greater than 0..7 micrometers size fraction.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954083.rdf
Name: Biomass_normalized_N_Uptake_Total
Units: per day
Description: <p>Biomass-normalized N Uptake Total: biomass-normalized nitrate uptake rates for both cell size-fractions.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954084.rdf
Name: C_Uptake_5um
Units: micromoles per liter per day (umol/L/day)
Description: <p>C Uptake >5um: absolute dissolved inorganic carbon (DIC) uptake rates for the greater than 5 micrometers size fraction.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954085.rdf
Name: C_Uptake_GFF
Units: micromoles per liter per day (umol/L/day)
Description: <p>C Uptake GFF: absolute dissolved inorganic carbon (DIC) uptake rates for the greater than 0..7 micrometers size fraction.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954086.rdf
Name: C_Uptake_Total
Units: micromoles per liter per day (umol/L/day)
Description: <p>C Uptake Total: absolute dissolved inorganic carbon (DIC) uptake rates for both cell size-fractions.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954087.rdf
Name: Biomass_normalized_C_Uptake_gt_5um
Units: per day
Description: <p>Biomass-normalized C Uptake >5um: biomass-normalized dissolved inorganic carbon (DIC) uptake rates for the greater than 5 micrometers size fraction.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954088.rdf
Name: Biomass_normalized_C_Uptake_GFF
Units: per day
Description: <p>Biomass-normalized C Uptake GFF: biomass-normalized dissolved inorganic carbon (DIC) uptake rates for the greater than 0..7 micrometers size fraction.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954089.rdf
Name: Biomass_normalized_C_Uptake_Total
Units: per day
Description: <p>Biomass-normalized C Uptake Total: biomass-normalized dissolved inorganic carbon (DIC) uptake rates for both cell size-fractions.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954090.rdf
Name: dissolved_Y
Units: picomoles per kilogram (pmol/kg)
Description: <p>Dissolved yttrium concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954091.rdf
Name: dissolved_Cd
Units: picomoles per kilogram (pmol/kg)
Description: <p>Dissolved cadmium concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954092.rdf
Name: dissolved_La
Units: picomoles per kilogram (pmol/kg)
Description: <p>Dissolved lanthanum concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954093.rdf
Name: dissolved_Pb
Units: picomoles per kilogram (pmol/kg)
Description: <p>Dissolved lead concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954094.rdf
Name: dissolved_Ce
Units: picomoles per kilogram (pmol/kg)
Description: <p>Dissolved cerium concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954095.rdf
Name: dissolved_Sc
Units: picomoles per kilogram (pmol/kg)
Description: <p>Dissolved scandium concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954096.rdf
Name: dissolved_Mn
Units: nanomoles per kilogram (nmol/kg)
Description: <p>Dissolved manganese concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954097.rdf
Name: dissolved_Fe
Units: nanomoles per kilogram (nmol/kg)
Description: <p>Dissolved iron concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954098.rdf
Name: dissolved_Co
Units: picomoles per kilogram (pmol/kg)
Description: <p>Dissolved cobalt concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954099.rdf
Name: dissolved_Ni
Units: nanomoles per kilogram (nmol/kg)
Description: <p>Dissolved nickel concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954100.rdf
Name: dissolved_Cu
Units: nanomoles per kilogram (nmol/kg)
Description: <p>Dissolved copper concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954101.rdf
Name: dissolved_Zn
Units: nanomoles per kilogram (nmol/kg)
Description: <p>Dissolved zinc concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954102.rdf
Name: soluble_Fe
Units: nanomoles per kilogram (nmol/kg)
Description: <p>Soluble iron concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954103.rdf
Name: soluble_Ni
Units: nanomoles per kilogram (nmol/kg)
Description: <p>Soluble nickel concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954104.rdf
Name: soluble_Cu
Units: nanomoles per kilogram (nmol/kg)
Description: <p>Soluble copper concentration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954105.rdf
Name: soluble_Zn
Units: nanomoles per kilogram (nmol/kg)
Description: <p>Soluble zinc concentration.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
13633
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/49cb097c-ec0c-4731-b19e-2a2eac4447a2/content
download
https://doi.org/10.26008/1912/bco-dmo.949801.1
download
onLine
dataset
<p><strong>Sample collection:</strong><br />
Four sites experiencing upwelling (E1: 41° 57.152 latitude, 124° 25.005 longitude. L2: 41° 42.078 latitude, 124° 30.865 longitude. L3: 43° 33.183 latitude, 124° 22.312 longitude. L4: 43° 59.988 latitude, 124° 41.28 longitude) were sampled off the coast of Northern California on May 30, 2019 (E1 and L2) and June 05, 2019 (L3 and L4) aboard the R/V Oceanus. Seawater was collected using a CTD-Rosette sampler, at four depths throughout the euphotic zone at each station corresponding to 50%, 30%, 10% and 1% incident irradiance (E1: 5.7 meters (m), 8.5m, 14.5m, 26.9m. L2: 5.7m, 9m, 16m, 27m. L3 and L4: 5m, 7m, 14m, 27m). At each depth, samples for chlorophyll a (Chl a), dissolved inorganic nutrients, dissolved inorganic carbon and nitrate uptake rates, cell counts and gene expression were obtained.</p>
<p><strong>Physiological Measurements:</strong><br />
For chlorophyll a measurements, 250 milliliters (mL) of seawater was gravity filtered through 5 micrometer (μm) Isopore membrane filters (47 millimeters (mm)) and subsequently vacuum filtered onto GF/F (25 mm) filters under 100 mmHg of vacuum pressure. Filters were then rinsed with 0.45 μm filtered seawater and immediately stored at -20 degrees Celsius (°C) until onshore analysis in the lab. Chlorophyll a extraction was performed using a 90% acetone solution at -20 °C for 24 hours and measured on a 10-AU fluorometer (Turner Designs, San Jose, CA) using the acidification method.</p>
<p>Dissolved inorganic nutrients (nitrate + nitrite, phosphate and silicic acid) were measured by filtering 30 mL of water through a 0.2 µm filter, using acid-washed syringes into an acid-cleaned polypropylene FalconTM tube. Dissolved nutrient concentrations were analyzed using an OI Analytical Flow Solutions IV auto analyzer by Wetland Biogeochemistry Analytical Services at Louisiana State University. Concentrations of nitrate measured from the discrete samples were used for the calculation of absolute nitrate uptake rates.</p>
<p>Samples for dissolved iron (dFe) were collected from each cubitainer within a trace-metal clean, positive pressure plastic bubble by filtering through pre-cleaned 0.2 um pore size polyethersulfone membrane Acropak-200® capsule filters into LDPE bottles that had been rigorously cleaned as described in the GEOTRACES cookbook. Sample bottles were rinsed three times with sample before filling. Samples were acidified at sea to pH ~1.7 with optima HCl (2 mL of 12 M HCl per liter of seawater) and were analyzed after the cruise. Briefly, this method involves pre-concentration onto Nobias-chelate PA1 resin followed by analysis with a High Resolution Inductively Coupled Plasma Mass Spectrometer. For quality control, a few samples were rerun with a flow injection analysis method. This method involves pre-concentration on toyopearl resin followed by in-line spectrophotometric analysis.</p>
<p>Seawater from the rosette was collected into polycarbonate bottles (~618 mL) and immediately spiked with both NaH13CO3 and Na15NO3 at approximately 10% of the predicted ambient DIC concentrations and measured nitrate concentrations under a trace metal clean (TMC) flow hood located on the ship. Samples were then incubated for six hours, and vacuum filtered similarly to Chl a measurements, such that cells greater than or equal to 5 μm were collected onto 5 μm Isopore polycarbonate membrane filters (47 mm), with the flow through containing cells smaller than 5 μm which were collected onto 0.7 μm pre-combusted GF/F filters. The polycarbonate filters containing cells greater than 5 μm were then rinsed onto new 0.7 μm pre-combusted GF/F filters using 0.45 μm filtered-seawater. The filters were then preserved at -20 °C until further processing in the laboratory. Prior to analysis, filters were dried at 60 °C for 24 hours, encapsulated in tin, and pelletized. Particulate organic carbon (POC), particulate organic nitrogen (PON), and atom percentages of 13C and 15N were subsequently quantified using an isotope ratio mass spectrometer (EA-IRMS) at the UC Davis Stable Isotope Facility. For each sample, POC and PON concentrations (micromoles per liter (μmol L-1)) were calculated by dividing the measured POC/PON mass (micrograms (μg)) by the respective atomic mass of carbon and nitrogen over the volume filtered (0.5 liter (L)).</p>
Specified by the Principal Investigator(s)
<p>GraphPad Prism was used to plot both the environmental and physiological data. RStudio was used to generate a Principal Component Analysis for the environmental and physiological data in order to determine correlation and variability among the four upwelling stations.</p>
Specified by the Principal Investigator(s)
- Imported original file "PUPCYCLE_compiled_results_Upwelling_transects.csv" into the BCO-DMO system.
- Renamed fields to comply with BCO-DMO naming conventions.
- Converted dates to YYYY-MM-DD format.
- Saved the final file as "949801_v1_pupcycle_2019_upwelling_transects.csv".
- This dataset has been published "as is". Final review by the data submitter was not received after the data were imported into the BCO-DMO data system.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
CTD-Rosette sampler
CTD-Rosette sampler
PI Supplied Instrument Name: CTD-Rosette sampler PI Supplied Instrument Description:Seawater was collected using a CTD-Rosette sampler. Instrument Name: CTD - profiler Instrument Short Name: Instrument Description: The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.
This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
High Resolution Inductively Coupled Plasma Mass Spectrometer
High Resolution Inductively Coupled Plasma Mass Spectrometer
PI Supplied Instrument Name: High Resolution Inductively Coupled Plasma Mass Spectrometer PI Supplied Instrument Description:Used in analysis of samples for dissolved iron (dFe). Instrument Name: Inductively Coupled Plasma Mass Spectrometer Instrument Short Name:ICP Mass Spec Instrument Description: An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB15/
isotope ratio mass spectrometer (EA-IRMS)
isotope ratio mass spectrometer (EA-IRMS)
PI Supplied Instrument Name: isotope ratio mass spectrometer (EA-IRMS) PI Supplied Instrument Description:Atom percentages of 13C and 15N were quantified using an isotope ratio mass spectrometer (EA-IRMS) at the UC Davis Stable Isotope Facility. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
OI Analytical Flow Solutions IV auto analyzer
OI Analytical Flow Solutions IV auto analyzer
PI Supplied Instrument Name: OI Analytical Flow Solutions IV auto analyzer PI Supplied Instrument Description:Dissolved inorganic nutrients were measured using the OI Analytical Flow Solutions IV auto analyzer by Wetland Biogeochemistry Analytical Services at Louisiana State University. Instrument Name: Nutrient Autoanalyzer Instrument Short Name:Nutrient Autoanalyzer Instrument Description: Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
10-AU fluorometers (Turner Designs)
10-AU fluorometers (Turner Designs)
PI Supplied Instrument Name: 10-AU fluorometers (Turner Designs) PI Supplied Instrument Description:Chlorophyll was measured using a 10-AU fluorometers (Turner Designs, San Jose, CA). Instrument Name: Turner Designs Fluorometer 10-AU Instrument Short Name:Turner Fluorometer 10-AU Instrument Description: The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/
Cruise: OC1905B
OC1905B
R/V Oceanus
Community Standard Description
International Council for the Exploration of the Sea
R/V Oceanus
vessel
OC1905B
Adrian Marchetti
R/V Oceanus
Community Standard Description
International Council for the Exploration of the Sea
R/V Oceanus
vessel