http://lod.bco-dmo.org/id/dataset/950296 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2025-02-03 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Depth profile chlorophyll, macronutrient, trace metal, metal speciation, and carbon system data from FeOA project cruise SKQ202209S on the R/V Sikuliaq in the Northeast Pacific between June and July 2022 2025-02-03 publication 2025-02-03 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2025-02-24 publication https://doi.org/10.26008/1912/bco-dmo.950296.1 Kristen Nicolle Buck University of South Florida principalInvestigator Mark L. Wells University of Maine principalInvestigator William P. Cochlan San Francisco State University principalInvestigator Yoshiko Kondo Nagasaki University principalInvestigator Shigenobu Takeda Nagasaki University principalInvestigator Charles Trick University of Western Ontario principalInvestigator Salvatore Caprara University of South Florida principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Buck, K. N., Parente, C. E., Kondo, Y., Cochlan, W. P., Caprara, S., Takeda, S., Trick, C., Wells, M. L. (2025) Depth profile chlorophyll, macronutrient, trace metal, metal speciation, and carbon system data from FeOA project cruise SKQ202209S on the R/V Sikuliaq in the Northeast Pacific between June and July 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-02-03 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.950296.1 [access date] FeOA Profile Data Dataset Description: Methods and Sampling: &lt;p&gt;&lt;strong&gt;Water column sampling:&lt;/strong&gt;&lt;br /&gt; The water column of eight stations in the NE Pacific was sampled to ~1,000 meters (m) in June 2022 aboard the R/V Sikuliaq. Depth profile samples were collected from a combination of the conventional ship rosette (SHIP CTD) and a trace metal clean rosette sampling system (TMC CTD) outfitted with 12-liter (L) modified x-Niskin (OceanTestEquipment) sampling bottles. Surface water (~2 m) samples for the depth profiles were collected using a trace metal clean surface pump &quot;towfish&quot; system (FISH; Mellett and Buck, 2020). Samples for dissolved macronutrients, dissolved trace metals, dissolved iron speciation, labile dissolved nickel concentrations, total alkalinity, pH, and chlorophyll a were collected primarily from the trace metal clean rosette and towfish; additional samples for dissolved macronutrients were also sampled from the conventional rosette. Amber high-density polyethylene bottles were filled directly from the towfish and from the x-Niskin samples immediately after each cast for parallel filtering (&amp;lt; 100 mm Hg) of chlorophyll a on 5-micrometer (µm) membrane (Poretics) filters and on 0.7 µm GF/F (Whatman) filters using a glass and stainless steel Millipore filtration rig in the main lab (William Cochlan lab). Filters for chlorophyll a were frozen at -20 degrees Celsius (°C) in the dark prior to their extraction and analysis at sea.&lt;/p&gt; &lt;p&gt;From the trace metal clean rosette and towfish, samples for dissolved macronutrients and trace metals were filtered (0.2 µm, Acropak) inline and collected inside a trace metal clean and positive pressure sampling bubble. Dissolved macronutrient samples collected from the conventional rosette were syringe filtered (0.2 µm PVDF syringe filters) in the Baltic room of the vessel. All dissolved macronutrient samples were collected into acid-cleaned and thrice sample rinsed 15-milliliter (mL) polypropylene Falcon tubes and stored in zipper bags in the fridge until analyzed shipboard following recommended practices (Becker et al., 2020), typically within 24 hours of collection (Caitlyn Parente, Kristen Buck lab). Samples for dissolved trace metals were collected in acid-cleaned and triple-rinsed narrow mouth low density polyethylene bottles, acidified with 0.024 M ultrapure hydrochloric acid (to pH ~1.8), and stored for shore-based analysis at the University of Nagasaki (Yoshiko Kondo, Shigenobu Takeda). Samples for dissolved iron speciation and labile dissolved nickel concentrations were collected in acid-cleaned, Milli-Q-conditioned, and triple-rinsed narrow-mouth fluorinated high-density polyethylene bottles (Nalgene) and analyzed shipboard for dissolved iron speciation (Lise Artigue, Kristen Buck lab) and labile dissolved nickel concentrations (Calyn Crawford, Kristen Buck lab) before freezing at -20 ºC for additional shore-based speciation analyses at Oregon State University. Samples for pH and total alkalinity were analyzed shipboard (Drajed Seto, Mark Wells lab).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sample analyses – macronutrients:&lt;/strong&gt;&lt;br /&gt; Filtered macronutrient samples were analyzed shipboard for phosphate, nitrate+nitrite, silicic acid, and nitrite on a QuAAtro39 AutoAnalyzer (SEAL Analytical) according to standard colorimetric methods (Strickland and Parsons, 1972). All reagents were prepared in dedicated labware with high purity Milli-Q (&amp;gt;18 MΩ cm) water. Working standards were prepared fresh daily in an artificial seawater (ASW; 35 grams per liter (g/L) sodium chloride, 0.5 g/L sodium bicarbonate) matrix using calibrated volumetric pipettes. Nine-point standard curves were analyzed at the beginning of each run with multiple reagent blanks. Quality control checks were analyzed every twelfth sample with ASW blanks and standards. The highest standard from the calibration curve was analyzed approximately every twenty samples to check for drift during the runs. Subsamples of reference material for nutrients in seawater (Kanso) were measured in each run. Detection limits for each parameter were determined from three times the standard deviation of replicate lowest standards. Limits of detection were 0.035 micromolar (µM) for phosphate, 0.048 µM for nitrate+nitrite, 0.051 µM for silicate, and 0.012 µM for nitrite. Values below these limits of detection are reported as 0 µM with accompanying QC Flag 6. Sample analyses for macronutrients were performed by MS student Caitlyn Parente.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sample analyses – chlorophyll a:&lt;/strong&gt;&lt;br /&gt; Samples for chlorophyll a were placed in glass test tubes and 8 mL of 100% ethanol was added to each tube (Jespersen and Christoffersen, 1987; Wasmund et al., 2006). The tubes were capped and placed in the dark for the extraction at room temperature. After 12 hours, the fluorescence readings were subsequently measured following the standard acidification protocol (Parsons et al., 1984; Arar and Collins, 1992) using a Turner Designs model 10-AU fluorometer calibrated at the beginning of the cruise with pure chlorophyll a standards (Turner Designs; Anacystis nidulans) following standard JGOFS protocols (Knap et al., 1996).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sample analyses – dissolved trace metals:&lt;/strong&gt;&lt;br /&gt; The concentrations of dissolved iron, manganese, nickel, zinc, and copper were analyzed by high-resolution inductively coupled plasma mass spectrometry (Thermo Scientific ELEMENT II) with a preconcentration flow injection system seaFAST-pico (Elemental Scientific Inc., ESI) at Nagasaki University (Yoshiko Kondo and Shigenobu Takeda). Acidified samples were measured without UV-oxidation, and dissolved copper concentrations should be considered ‘reactive Cu’ as recovery may have been hindered by organic complexation in these samples. Briefly, dissolved trace metals in the samples were preconcentrated on a Nobias-chelate PA1 resin, eluted with 2 M HNO3, and quantified by calibration curve prepared with SAFe and GEOTRACES reference samples (S1, GS, and GD) (&lt;a href=&quot;https://www.geotraces.org/standards-and-reference-materials/&quot; target=&quot;_blank&quot;&gt;https://www.geotraces.org/standards-and-reference-materials/&lt;/a&gt;).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sample analyses – labile dissolved nickel:&lt;/strong&gt;&lt;br /&gt; The concentration of labile dissolved nickel concentrations was measured by competitive ligand exchange-adsorptive cathodic stripping voltammetry using the added ligand dimethylglyoxime (DMG; van den Berg and Nimmo 1987) and following a modification of previously described procedures (Saito et al. 2004; Boiteau et al. 2016). Briefly, seawater sample aliquots were buffered with a borate-ammonium buffer and equilibrated overnight with 200 µM DMG. Following equilibration, the amount of dissolved nickel in the samples that was bound to DMG was measured on a hanging mercury drop electrode and quantified by standard additions of dissolved nickel to the sample. All measurements, of the sample and of the standard additions, were conducted in triplicate. The concentration of labile dissolved nickel was determined from the slope of the standard curve and the triplicate measurements of the initial sample, and the results presented as averages and standard deviations of the three values.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sample analyses – total alkalinity:&lt;/strong&gt;&lt;br /&gt; Total alkalinity (TA) was measured using a USB4000 fiber optic spectrometer (Ocean Optics) with bromocresol purple (BCP) as the indicator dye (Hudson-Heck et al., 2021). The spectrometer was calibrated daily using certified reference materials (batch number 189) obtained from the Dickson lab at the Scripps Institution of Oceanography. Calibration was performed at a controlled temperature of 20°C to match the temperature of the spectrophotometric cuvette, which was maintained using a water bath. Seawater samples (2 mL) were pipetted into pre-rinsed (deionized water) and dried spectrophotometric cuvettes and BCP indicator solution was added. The cuvette was capped and purged with N2 gas for 1 minute and the absorbance spectra were recorded at 432, 589, and 700 nanometers (nm). TA was calculated using the absorbance readings with the final absorbance ratio (R ratio) for each sample (Hudson-Heck et al., 2021). All samples were analyzed in triplicate and the results presented as the averages and standard deviations of the three values.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sample analyses – pH:&lt;/strong&gt;&lt;br /&gt; pH was measured using a USB4000 fiber optic spectrometer (Ocean Optics) with purified meta-Cresol Purple (mCP) as the pH indicator dye (Liu et al., 2011). The system comprised an open top, flow-thru cell positioned in a temperature-controlled (20 °C) water bath. The cell was zeroed by manually injecting a blank or reference sample (seawater without mCP) and recording the absorbance at 434, 578, and 700 nm (reference). For sample analysis, 1 microliter (µL) of purified mCP indicator solution was drawn into a clean 3 mL syringe followed by 2 mL of seawater sample. The solution was mixed gently to ensure uniform distribution of the indicator while avoiding air bubble formation. The solution then was manually injected into the flow-thru cell (using excess volumes for rinse) and allowed to thermally equilibrate. Once absorbance values had stabilized (1-3 minutes) the values were recorded at 434, 578, and 700 nm. Seawater pH was calculated on the total scale using the absorbance ratio (578/434) according to Liu et al. (2011). All samples were analyzed in triplicate and the results presented as the averages and standard deviations of the three values.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1830029 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1830029 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1829753 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1829753 completed Kristen Nicolle Buck University of South Florida 541-737-7401 2651 SW Orchard Avenue CEOAS Corvallis OR 97331 USA kristen.buck@oregonstate.edu pointOfContact Mark L. Wells University of Maine 207-581-4322 School of Marine Sciences Rm 201 Libby Hall Orono ME 04469 USA mlwells@maine.edu pointOfContact William P. Cochlan San Francisco State University 415-338-3541 Romberg Tiburon Center San Francisco State University 3152 Paradise Drive Tiburon CA 94920-1205 USA cochlan@sfsu.edu pointOfContact Yoshiko Kondo Nagasaki University yoshikondo@nagasaki-u.ac.jp pointOfContact Shigenobu Takeda Nagasaki University Japan s-takeda@nagasaki-u.ac.jp pointOfContact Charles Trick University of Western Ontario 519-661-2111 ext. 83899 North Campus Building Rm 402 London Ontario N6A 3K7 Canada trick@uwo.ca pointOfContact Salvatore Caprara University of South Florida capraras@oregonstate.edu pointOfContact asNeeded Dataset Version: 1 Unknown CRUISE_ID EVTNBR DATE_SHIP TIME_IN_SHIP TIME_OUT_SHIP IN_ISO_DateTime_UTC OUT_ISO_DateTime_UTC LATITUDE LONGITUDE PLATFORM CASTNBR STNNBR BTLNBR DEPTH SIGMA_T NUTS_ID NO3_NO2_CONC NO3_NO2_STDEV NO3_NO2_PCNT_RSD NO3_NO2_COUNT NO3_NO2_FLAG PO4_CONC PO4_STDEV PO4_PCNT_RSD PO4_COUNT PO4_FLAG SiO4_CONC SiO4_STDEV SiO4_PCNT_RSD SiO4_COUNT SiO4_FLAG NO2_CONC NO2_STDEV NO2_PCNT_RSD NO2_COUNT NO2_FLAG CHLA_FLUOR_TP_CONC CHLA_FLUOR_TP_FLAG PHAEO_FLUOR_TP_CONC PHAEO_FLUOR_TP_FLAG CHLA_FLUOR_LP_CONC CHLA_FLUOR_LP_FLAG PHAEO_FLUOR_LP_CONC PHAEO_FLUOR_LP_FLAG Fe_D_CONC Fe_D_CONC_FLAG Mn_D_CONC Mn_D_CONC_FLAG Ni_D_CONC Ni_D_CONC_FLAG Zn_D_CONC Zn_D_CONC_FLAG Cu_D_CONC Cu_D_CONC_FLAG Ni_DL_CONC Ni_DL_STDEV Ni_DL_CONC_FLAG pH pH_STDEV pH_FLAG TA TA_STDEV TA_FLAG BioAnalytical Systems Inc. controlled growth mercury electrode Epsilon Eclipse voltametric analyzer ThermoScientific Element II Niskin bottle samplers QuAAtro39 AutoAnalyzer Elemental Scientific seaFAST-pico system Ocean Optics USB4000 fiber optic spectrometer Towfish Turner AU10 fluorometer theme None, User defined Cruise identfier event date time of day ISO_DateTime_UTC latitude longitude instrument cast station bottle depth sigma-theta sample identification nitrate plus nitrite number quality flag reactive phosphorus (PO4) Silicate (reactive silica) Nitrite chlorophyll a total phaeopigment Iron Manganese Nickel Zinc Copper pH total alkalinity (TA) featureType BCO-DMO Standard Parameters BASi Controlled Growth Mercury Electrode BASi EC-epsilon 2 Autoanalyzer Inductively Coupled Plasma Mass Spectrometer Niskin bottle Nutrient Autoanalyzer SeaFAST Automated Preconcentration System Spectrometer towed unmanned submersible Turner Designs Fluorometer-10 instrument BCO-DMO Standard Instruments SKQ202209S service Deployment Activity Northeast Pacific Ocean; 35 to 55 N, -125 to -145 E place Locations otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: The Effect of Ocean Acidification on Fe Availability to Phytoplankton in Coastal and Oceanic Waters of the Eastern North Pacific https://osprey.bco-dmo.org/project/784135 Collaborative Research: The Effect of Ocean Acidification on Fe Availability to Phytoplankton in Coastal and Oceanic Waters of the Eastern North Pacific <p><em>NSF Award Abstract:</em><br /> Iron is an important nutrient for algae in the ocean. Different forms of iron and their availability to algae are influenced by many factors including the acidity of seawater (or pH). This research project focuses on understanding the effects of ocean acidification (low pH) on the associations between iron and chemical substances that bind with iron in seawater. The investigators will work in coastal and oceanic waters of the Pacific Ocean. These waters are characterized by substances that have weak and strong associations with iron. Samples will be collected from coastal waters off Washington State, the northern edge of the North Pacific gyre, and Ocean Station PAPA in the northeast subarctic Pacific. Water samples will be collected to test phytoplankton responses to light, pH, forms of iron, and the composition of the substances that bind with iron. This project will support graduate and undergraduate students. The investigators will participate in a range of education and outreach activities.</p> <p>This study addresses oceanic responses to rising anthropogenic CO2 and is broadly relevant to ocean biogeochemistry. The investigators will study the role of ocean acidification on iron availability in the Eastern North Pacific Ocean. The study location is characterized as a high nutrient low chlorophyll (HNLC) region of the ocean, where phytoplankton may be particularly sensitive to iron availability. The study region is also characterized by gradients in ligand composition and binding strength. This study will investigate how the associations between iron and different ligands (organic compounds that bind with iron) are influenced by pH and how this, in turn, influences primary production and microbial community structure in the ocean. The investigators will use batch cultures, at pH 8.1 and 7.6, and under high and low light regimes, to examine the iron demand of phytoplankton. Understanding how pH influences iron and its relationship with ligands will provide important information for assessing the impacts of ocean acidification on primary production and biogeochemical processes.</p> pH-Fe availability largerWorkCitation project eng; USA oceans Northeast Pacific Ocean; 35 to 55 N, -125 to -145 E -145.0213 -125.5083 34.9997 50.0046 2022-06-09 2022-06-28 North East Pacific, Ocean Station PAPA 0 BCO-DMO catalogue of parameters from Depth profile chlorophyll, macronutrient, trace metal, metal speciation, and carbon system data from FeOA project cruise SKQ202209S on the R/V Sikuliaq in the Northeast Pacific between June and July 2022 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/952910.rdf Name: CRUISE_ID Units: unitless Description: <p>Cruise identifier</p> http://lod.bco-dmo.org/id/dataset-parameter/952911.rdf Name: EVTNBR Units: unitless Description: <p>Event number from R2R log</p> http://lod.bco-dmo.org/id/dataset-parameter/952912.rdf Name: DATE_SHIP Units: unitless Description: <p>Date in ship time when sampling was initiated</p> http://lod.bco-dmo.org/id/dataset-parameter/952913.rdf Name: TIME_IN_SHIP Units: unitless Description: <p>Ship time when field sampling platform was deployed. (Note the ship moved from Seattle (Pacific Time) to Alaska over the course of the cruise. Thus, the difference between ship time and UTC changes from -7 to -8 hours.)</p> http://lod.bco-dmo.org/id/dataset-parameter/952914.rdf Name: TIME_OUT_SHIP Units: unitless Description: <p>Ship time when field sampling platform was recovered. (Note the ship moved from Seattle (Pacific Time) to Alaska over the course of the cruise. Thus, the difference between ship time and UTC changes from -7 to -8 hours.)</p> http://lod.bco-dmo.org/id/dataset-parameter/952915.rdf Name: IN_ISO_DateTime_UTC Units: unitless Description: <p>Date and time in UTC when field sampling platform was recovered</p> http://lod.bco-dmo.org/id/dataset-parameter/952916.rdf Name: OUT_ISO_DateTime_UTC Units: unitless Description: <p>Date and time in UTC when field sampling platform was deployed</p> http://lod.bco-dmo.org/id/dataset-parameter/952917.rdf Name: LATITUDE Units: decimal degrees Description: <p>Ship position when sample was collected in decimal °N</p> http://lod.bco-dmo.org/id/dataset-parameter/952918.rdf Name: LONGITUDE Units: decimal degrees Description: <p>Ship position when sample was collected in decimal °E</p> http://lod.bco-dmo.org/id/dataset-parameter/952919.rdf Name: PLATFORM Units: unitless Description: <p>Sampling system used. TMC CTD = trace metal CTD rosette. FISH = tow fish. SHIP CTD = ship conventional CTD rosette</p> http://lod.bco-dmo.org/id/dataset-parameter/952920.rdf Name: CASTNBR Units: unitless Description: <p>Cast number assigned in R2R event log</p> http://lod.bco-dmo.org/id/dataset-parameter/952921.rdf Name: STNNBR Units: unitless Description: <p>Station number</p> http://lod.bco-dmo.org/id/dataset-parameter/952922.rdf Name: BTLNBR Units: unitless Description: <p>Niskin bottle number (CTD# for SHIP CTD or N# for TMC CTD) used for sample collection. Samples were collected directly inline from towfish and noted FISH.</p> http://lod.bco-dmo.org/id/dataset-parameter/952923.rdf Name: DEPTH Units: meters (m) Description: <p>Depth in meters of sample collection</p> http://lod.bco-dmo.org/id/dataset-parameter/952924.rdf Name: SIGMA_T Units: kilograms per cubic meter (kg/m3) Description: <p>Potential density anomaly associated with each sample, retrieved from CTD profiler for depth of Niskin bottle closure. ‘nda’ for ‘no data available’ used when data has not yet been obtained for this parameter.</p> http://lod.bco-dmo.org/id/dataset-parameter/952925.rdf Name: NUTS_ID Units: unitless Description: <p>Unique number assigned to all polypropylene falcon tubes containing dissolved macronutrient samples.</p> http://lod.bco-dmo.org/id/dataset-parameter/952926.rdf Name: NO3_NO2_CONC Units: micromoles per liter (uM) Description: <p>Concentrations of dissolved nitrate+nitrite in field samples.</p> http://lod.bco-dmo.org/id/dataset-parameter/952927.rdf Name: NO3_NO2_STDEV Units: micromoles per liter (uM) Description: <p>Standard deviation of replicate nitrate+nitrite concentration measurements. If only 2 replicates, the difference about the mean was used to calculate error.</p> http://lod.bco-dmo.org/id/dataset-parameter/952928.rdf Name: NO3_NO2_PCNT_RSD Units: percent (%) Description: <p>Percent relative standard deviation of replicate nitrate+nitrite concentration measurements. Calculated as NO3_NO2_STDEV divided by NO3_NO2 and multiplied by 100; ‘na’ for ‘not applicable’, used when value of 0 assigned to concentrations &lt;LOD.</p> http://lod.bco-dmo.org/id/dataset-parameter/952929.rdf Name: NO3_NO2_COUNT Units: unitless Description: <p>Number of separate analyses of this sample used to compute average concentration, standard deviation, and % RSD.</p> http://lod.bco-dmo.org/id/dataset-parameter/952930.rdf Name: NO3_NO2_FLAG Units: unitless Description: <p>Quality flag for NO3_NO2.</p> http://lod.bco-dmo.org/id/dataset-parameter/952931.rdf Name: PO4_CONC Units: micromoles per liter (uM) Description: <p>Concentrations of dissolved phosphate in field samples.</p> http://lod.bco-dmo.org/id/dataset-parameter/952932.rdf Name: PO4_STDEV Units: micromoles per liter (uM) Description: <p>Standard deviation of replicate phosphate concentration measurements. If only 2 replicates, the difference about the mean was used to calculate error.</p> http://lod.bco-dmo.org/id/dataset-parameter/952933.rdf Name: PO4_PCNT_RSD Units: percent (%) Description: <p>Percent relative standard deviation of replicate phosphate concentration measurements. Calculated as PO4_STDEV divided by PO4 and multiplied by 100; ‘na’ for ‘not applicable’, used when value of 0 assigned to concentrations &lt;LOD.</p> http://lod.bco-dmo.org/id/dataset-parameter/952934.rdf Name: PO4_COUNT Units: unitless Description: <p>Number of separate analyses of this sample used to compute average concentration, standard deviation, and % RSD.</p> http://lod.bco-dmo.org/id/dataset-parameter/952935.rdf Name: PO4_FLAG Units: unitless Description: <p>Quality flag for PO4_CONC. 6 = below limit of detection</p> http://lod.bco-dmo.org/id/dataset-parameter/952936.rdf Name: SiO4_CONC Units: micromoles per liter (uM) Description: <p>Concentrations of dissolved silicic acid in field samples.</p> http://lod.bco-dmo.org/id/dataset-parameter/952937.rdf Name: SiO4_STDEV Units: micromoles per liter (uM) Description: <p>Standard deviation of replicate silicate concentration measurements. If only 2 replicates, the difference about the mean was used to calculate error.</p> http://lod.bco-dmo.org/id/dataset-parameter/952938.rdf Name: SiO4_PCNT_RSD Units: percent (%) Description: <p>Percent relative standard deviation of replicate silicate concentration measurements. Calculated as SiO4_STDEV divided by SiO4 and multiplied by 100.</p> http://lod.bco-dmo.org/id/dataset-parameter/952939.rdf Name: SiO4_COUNT Units: unitless Description: <p>Number of separate analyses of this sample used to compute average concentration, standard deviation, and % RSD.</p> http://lod.bco-dmo.org/id/dataset-parameter/952940.rdf Name: SiO4_FLAG Units: unitless Description: <p>Quality flag for SiO4.</p> http://lod.bco-dmo.org/id/dataset-parameter/952941.rdf Name: NO2_CONC Units: micromoles per liter (uM) Description: <p>Concentrations of dissolved nitrite in field samples.</p> http://lod.bco-dmo.org/id/dataset-parameter/952942.rdf Name: NO2_STDEV Units: micromoles per liter (uM) Description: <p>Standard deviation of replicate nitrate+nitrite concentration measurements. If only 2 replicates, the difference about the mean was used to calculate error.</p> http://lod.bco-dmo.org/id/dataset-parameter/952943.rdf Name: NO2_PCNT_RSD Units: percent (%) Description: <p>Percent relative standard deviation of replicate nitrite concentration measurements. Calculated as NO2_STDEV divided by NO2 and multiplied by 100; ‘na’ for ‘not applicable’, used when value of 0 assigned to concentrations &lt;LOD.</p> http://lod.bco-dmo.org/id/dataset-parameter/952944.rdf Name: NO2_COUNT Units: unitless Description: <p>Number of separate analyses of this sample used to compute average concentration, standard deviation, and % RSD.</p> http://lod.bco-dmo.org/id/dataset-parameter/952945.rdf Name: NO2_FLAG Units: unitless Description: <p>Quality flag for NO2.</p> http://lod.bco-dmo.org/id/dataset-parameter/952946.rdf Name: CHLA_FLUOR_TP_CONC Units: micrograms per liter (ug/L) Description: <p>Concentrations of total chlorophyll a on GF/F filters from field samples. ‘na’ for ‘not applicable’ used when no sample was collected for this parameter.</p> http://lod.bco-dmo.org/id/dataset-parameter/952947.rdf Name: CHLA_FLUOR_TP_FLAG Units: unitless Description: <p>Quality flag for CHLA_FLUOR_TP.</p> http://lod.bco-dmo.org/id/dataset-parameter/952948.rdf Name: PHAEO_FLUOR_TP_CONC Units: micrograms per liter (ug/L) Description: <p>Concentrations of total phaeophytin on GF/F filters from field samples. ‘na’ for ‘not applicable’ used when no sample was collected for this parameter.</p> http://lod.bco-dmo.org/id/dataset-parameter/952949.rdf Name: PHAEO_FLUOR_TP_FLAG Units: unitless Description: <p>Quality flag for PHAEO_FLUOR_TP.</p> http://lod.bco-dmo.org/id/dataset-parameter/952950.rdf Name: CHLA_FLUOR_LP_CONC Units: micrograms per liter (ug/L) Description: <p>Concentrations of large particle chlorophyll a collected on 5 µm filters from field samples. ‘na’ for ‘not applicable’ used when no sample was collected for this parameter.</p> http://lod.bco-dmo.org/id/dataset-parameter/952951.rdf Name: CHLA_FLUOR_LP_FLAG Units: unitless Description: <p>Quality flag for CHLA_FLUOR_LP.</p> http://lod.bco-dmo.org/id/dataset-parameter/952952.rdf Name: PHAEO_FLUOR_LP_CONC Units: micrograms per liter (ug/L) Description: <p>Concentrations of large particle phaeophytin on 5 µm filters from field samples. ‘na’ for ‘not applicable’ used when no sample was collected for this parameter.</p> http://lod.bco-dmo.org/id/dataset-parameter/952953.rdf Name: PHAEO_FLUOR_LP_FLAG Units: unitless Description: <p>Quality flag for PHAEO_FLUOR_LP.</p> http://lod.bco-dmo.org/id/dataset-parameter/952954.rdf Name: Fe_D_CONC Units: nanomoles per liter (nmol/L) Description: <p>Concentrations of dissolved iron (Fe) in field samples. ‘na’ for ‘not applicable’ used when no sample was collected for this parameter. ‘nda’ for ‘no data available’ used when sample was collected but no data has been obtained for this parameter.</p> http://lod.bco-dmo.org/id/dataset-parameter/952955.rdf Name: Fe_D_CONC_FLAG Units: unitless Description: <p>Quality flag for Fe_D_CONC.</p> http://lod.bco-dmo.org/id/dataset-parameter/952956.rdf Name: Mn_D_CONC Units: nanomoles per liter (nmol/L) Description: <p>Concentrations of dissolved manganese (Mn) in field samples. ‘na’ for ‘not applicable’ used when no sample was collected for this parameter. ‘nda’ for ‘no data available’ used when sample was collected but no data has been obtained for this parameter.</p> http://lod.bco-dmo.org/id/dataset-parameter/952957.rdf Name: Mn_D_CONC_FLAG Units: unitless Description: <p>Quality flag for Mn_D_CONC.</p> http://lod.bco-dmo.org/id/dataset-parameter/952958.rdf Name: Ni_D_CONC Units: nanomoles per liter (nmol/L) Description: <p>Concentrations of dissolved nickel (Ni) in field samples. ‘na’ for ‘not applicable’ used when no sample was collected for this parameter. ‘nda’ for ‘no data available’ used when sample was collected but no data has been obtained for this parameter.</p> http://lod.bco-dmo.org/id/dataset-parameter/952959.rdf Name: Ni_D_CONC_FLAG Units: unitless Description: <p>Quality flag for Ni_D_CONC.</p> http://lod.bco-dmo.org/id/dataset-parameter/952960.rdf Name: Zn_D_CONC Units: nanomoles per liter (nmol/L) Description: <p>Concentrations of dissolved zinc (Zn) in field samples. ‘na’ for ‘not applicable’ used when no sample was collected for this parameter. ‘nda’ for ‘no data available’ used when sample was collected but no data has been obtained for this parameter.</p> http://lod.bco-dmo.org/id/dataset-parameter/952961.rdf Name: Zn_D_CONC_FLAG Units: unitless Description: <p>Quality flag for Zn_D_CONC.</p> http://lod.bco-dmo.org/id/dataset-parameter/952962.rdf Name: Cu_D_CONC Units: nanomoles per liter (nmol/L) Description: <p>Concentrations of dissolved copper (Cu) in field samples. ‘na’ for ‘not applicable’ used when no sample was collected for this parameter. ‘nda’ for ‘no data available’ used when sample was collected but no data has been obtained for this parameter.</p> http://lod.bco-dmo.org/id/dataset-parameter/952963.rdf Name: Cu_D_CONC_FLAG Units: unitless Description: <p>Quality flag for Cu_D_CONC.</p> http://lod.bco-dmo.org/id/dataset-parameter/952964.rdf Name: Ni_DL_CONC Units: nanomoles per liter (nmol/L) Description: <p>Concentrations of labile dissolved nickel (Ni) in field samples. ‘na’ for ‘not applicable’ used when no sample was collected for this parameter. ‘nda’ for ‘no data available’ used when sample was collected but no data has been obtained for this parameter.</p> http://lod.bco-dmo.org/id/dataset-parameter/952965.rdf Name: Ni_DL_STDEV Units: nanomoles per liter (nmol/L) Description: <p>Standard deviation for Ni_DL_CONC</p> http://lod.bco-dmo.org/id/dataset-parameter/952966.rdf Name: Ni_DL_CONC_FLAG Units: unitless Description: <p>Quality flag for Ni_DL_CONC.</p> http://lod.bco-dmo.org/id/dataset-parameter/952967.rdf Name: pH Units: unitless Description: <p>pH of field samples. ‘na’ for ‘not applicable’ used when no sample was collected for this parameter. ‘nda’ for ‘no data available’ used when sample was collected but no data has been obtained for this parameter.</p> http://lod.bco-dmo.org/id/dataset-parameter/952968.rdf Name: pH_STDEV Units: unitless Description: <p>Standard deviation of replicate pH measurements. ‘na’ for ‘not applicable’ used when no sample was collected for this parameter. ‘nda’ for ‘no data available’ used when sample was collected but no data has been obtained for this parameter.</p> http://lod.bco-dmo.org/id/dataset-parameter/952969.rdf Name: pH_FLAG Units: unitless Description: <p>Quality flag for pH.</p> http://lod.bco-dmo.org/id/dataset-parameter/952970.rdf Name: TA Units: milligrams per liter (mg/L) Description: <p>Total alkalinity</p> http://lod.bco-dmo.org/id/dataset-parameter/952971.rdf Name: TA_STDEV Units: milligrams per liter (mg/L) Description: <p>Standard deviation for TA.</p> http://lod.bco-dmo.org/id/dataset-parameter/952972.rdf Name: TA_FLAG Units: unitless Description: <p>Quality flag for TA.</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 68367 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/7ee8c56c-a255-4456-8f4c-4dbcdae46c92/content download https://doi.org/10.26008/1912/bco-dmo.950296.1 download onLine dataset &lt;p&gt;&lt;strong&gt;Water column sampling:&lt;/strong&gt;&lt;br /&gt; The water column of eight stations in the NE Pacific was sampled to ~1,000 meters (m) in June 2022 aboard the R/V Sikuliaq. Depth profile samples were collected from a combination of the conventional ship rosette (SHIP CTD) and a trace metal clean rosette sampling system (TMC CTD) outfitted with 12-liter (L) modified x-Niskin (OceanTestEquipment) sampling bottles. Surface water (~2 m) samples for the depth profiles were collected using a trace metal clean surface pump &quot;towfish&quot; system (FISH; Mellett and Buck, 2020). Samples for dissolved macronutrients, dissolved trace metals, dissolved iron speciation, labile dissolved nickel concentrations, total alkalinity, pH, and chlorophyll a were collected primarily from the trace metal clean rosette and towfish; additional samples for dissolved macronutrients were also sampled from the conventional rosette. Amber high-density polyethylene bottles were filled directly from the towfish and from the x-Niskin samples immediately after each cast for parallel filtering (&amp;lt; 100 mm Hg) of chlorophyll a on 5-micrometer (µm) membrane (Poretics) filters and on 0.7 µm GF/F (Whatman) filters using a glass and stainless steel Millipore filtration rig in the main lab (William Cochlan lab). Filters for chlorophyll a were frozen at -20 degrees Celsius (°C) in the dark prior to their extraction and analysis at sea.&lt;/p&gt; &lt;p&gt;From the trace metal clean rosette and towfish, samples for dissolved macronutrients and trace metals were filtered (0.2 µm, Acropak) inline and collected inside a trace metal clean and positive pressure sampling bubble. Dissolved macronutrient samples collected from the conventional rosette were syringe filtered (0.2 µm PVDF syringe filters) in the Baltic room of the vessel. All dissolved macronutrient samples were collected into acid-cleaned and thrice sample rinsed 15-milliliter (mL) polypropylene Falcon tubes and stored in zipper bags in the fridge until analyzed shipboard following recommended practices (Becker et al., 2020), typically within 24 hours of collection (Caitlyn Parente, Kristen Buck lab). Samples for dissolved trace metals were collected in acid-cleaned and triple-rinsed narrow mouth low density polyethylene bottles, acidified with 0.024 M ultrapure hydrochloric acid (to pH ~1.8), and stored for shore-based analysis at the University of Nagasaki (Yoshiko Kondo, Shigenobu Takeda). Samples for dissolved iron speciation and labile dissolved nickel concentrations were collected in acid-cleaned, Milli-Q-conditioned, and triple-rinsed narrow-mouth fluorinated high-density polyethylene bottles (Nalgene) and analyzed shipboard for dissolved iron speciation (Lise Artigue, Kristen Buck lab) and labile dissolved nickel concentrations (Calyn Crawford, Kristen Buck lab) before freezing at -20 ºC for additional shore-based speciation analyses at Oregon State University. Samples for pH and total alkalinity were analyzed shipboard (Drajed Seto, Mark Wells lab).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sample analyses – macronutrients:&lt;/strong&gt;&lt;br /&gt; Filtered macronutrient samples were analyzed shipboard for phosphate, nitrate+nitrite, silicic acid, and nitrite on a QuAAtro39 AutoAnalyzer (SEAL Analytical) according to standard colorimetric methods (Strickland and Parsons, 1972). All reagents were prepared in dedicated labware with high purity Milli-Q (&amp;gt;18 MΩ cm) water. Working standards were prepared fresh daily in an artificial seawater (ASW; 35 grams per liter (g/L) sodium chloride, 0.5 g/L sodium bicarbonate) matrix using calibrated volumetric pipettes. Nine-point standard curves were analyzed at the beginning of each run with multiple reagent blanks. Quality control checks were analyzed every twelfth sample with ASW blanks and standards. The highest standard from the calibration curve was analyzed approximately every twenty samples to check for drift during the runs. Subsamples of reference material for nutrients in seawater (Kanso) were measured in each run. Detection limits for each parameter were determined from three times the standard deviation of replicate lowest standards. Limits of detection were 0.035 micromolar (µM) for phosphate, 0.048 µM for nitrate+nitrite, 0.051 µM for silicate, and 0.012 µM for nitrite. Values below these limits of detection are reported as 0 µM with accompanying QC Flag 6. Sample analyses for macronutrients were performed by MS student Caitlyn Parente.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sample analyses – chlorophyll a:&lt;/strong&gt;&lt;br /&gt; Samples for chlorophyll a were placed in glass test tubes and 8 mL of 100% ethanol was added to each tube (Jespersen and Christoffersen, 1987; Wasmund et al., 2006). The tubes were capped and placed in the dark for the extraction at room temperature. After 12 hours, the fluorescence readings were subsequently measured following the standard acidification protocol (Parsons et al., 1984; Arar and Collins, 1992) using a Turner Designs model 10-AU fluorometer calibrated at the beginning of the cruise with pure chlorophyll a standards (Turner Designs; Anacystis nidulans) following standard JGOFS protocols (Knap et al., 1996).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sample analyses – dissolved trace metals:&lt;/strong&gt;&lt;br /&gt; The concentrations of dissolved iron, manganese, nickel, zinc, and copper were analyzed by high-resolution inductively coupled plasma mass spectrometry (Thermo Scientific ELEMENT II) with a preconcentration flow injection system seaFAST-pico (Elemental Scientific Inc., ESI) at Nagasaki University (Yoshiko Kondo and Shigenobu Takeda). Acidified samples were measured without UV-oxidation, and dissolved copper concentrations should be considered ‘reactive Cu’ as recovery may have been hindered by organic complexation in these samples. Briefly, dissolved trace metals in the samples were preconcentrated on a Nobias-chelate PA1 resin, eluted with 2 M HNO3, and quantified by calibration curve prepared with SAFe and GEOTRACES reference samples (S1, GS, and GD) (&lt;a href=&quot;https://www.geotraces.org/standards-and-reference-materials/&quot; target=&quot;_blank&quot;&gt;https://www.geotraces.org/standards-and-reference-materials/&lt;/a&gt;).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sample analyses – labile dissolved nickel:&lt;/strong&gt;&lt;br /&gt; The concentration of labile dissolved nickel concentrations was measured by competitive ligand exchange-adsorptive cathodic stripping voltammetry using the added ligand dimethylglyoxime (DMG; van den Berg and Nimmo 1987) and following a modification of previously described procedures (Saito et al. 2004; Boiteau et al. 2016). Briefly, seawater sample aliquots were buffered with a borate-ammonium buffer and equilibrated overnight with 200 µM DMG. Following equilibration, the amount of dissolved nickel in the samples that was bound to DMG was measured on a hanging mercury drop electrode and quantified by standard additions of dissolved nickel to the sample. All measurements, of the sample and of the standard additions, were conducted in triplicate. The concentration of labile dissolved nickel was determined from the slope of the standard curve and the triplicate measurements of the initial sample, and the results presented as averages and standard deviations of the three values.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sample analyses – total alkalinity:&lt;/strong&gt;&lt;br /&gt; Total alkalinity (TA) was measured using a USB4000 fiber optic spectrometer (Ocean Optics) with bromocresol purple (BCP) as the indicator dye (Hudson-Heck et al., 2021). The spectrometer was calibrated daily using certified reference materials (batch number 189) obtained from the Dickson lab at the Scripps Institution of Oceanography. Calibration was performed at a controlled temperature of 20°C to match the temperature of the spectrophotometric cuvette, which was maintained using a water bath. Seawater samples (2 mL) were pipetted into pre-rinsed (deionized water) and dried spectrophotometric cuvettes and BCP indicator solution was added. The cuvette was capped and purged with N2 gas for 1 minute and the absorbance spectra were recorded at 432, 589, and 700 nanometers (nm). TA was calculated using the absorbance readings with the final absorbance ratio (R ratio) for each sample (Hudson-Heck et al., 2021). All samples were analyzed in triplicate and the results presented as the averages and standard deviations of the three values.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sample analyses – pH:&lt;/strong&gt;&lt;br /&gt; pH was measured using a USB4000 fiber optic spectrometer (Ocean Optics) with purified meta-Cresol Purple (mCP) as the pH indicator dye (Liu et al., 2011). The system comprised an open top, flow-thru cell positioned in a temperature-controlled (20 °C) water bath. The cell was zeroed by manually injecting a blank or reference sample (seawater without mCP) and recording the absorbance at 434, 578, and 700 nm (reference). For sample analysis, 1 microliter (µL) of purified mCP indicator solution was drawn into a clean 3 mL syringe followed by 2 mL of seawater sample. The solution was mixed gently to ensure uniform distribution of the indicator while avoiding air bubble formation. The solution then was manually injected into the flow-thru cell (using excess volumes for rinse) and allowed to thermally equilibrate. Once absorbance values had stabilized (1-3 minutes) the values were recorded at 434, 578, and 700 nm. Seawater pH was calculated on the total scale using the absorbance ratio (578/434) according to Liu et al. (2011). All samples were analyzed in triplicate and the results presented as the averages and standard deviations of the three values.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;&lt;strong&gt;Data Quality Flags:&lt;/strong&gt;&lt;br /&gt; Data were flagged using the SeaDataNet quality flag scheme recommended by GEOTRACES (&lt;a href=&quot;https://www.geotraces.org/geotraces-quality-flag-policy/&quot; target=&quot;_blank&quot;&gt;https://www.geotraces.org/geotraces-quality-flag-policy/&lt;/a&gt;) and described below. Notes specific to the application of these flags to this dataset are noted in brackets […].&lt;/p&gt; &lt;p&gt;0: No Quality Control: No quality control procedures have been applied to the data value. This is the initial status for all data values entering the working archive. [Not used].&lt;/p&gt; &lt;p&gt;1: Good Value: Good quality data value that is not part of any identified malfunction and has been verified as consistent with real phenomena during the quality control process. [Used for analyses that included replicates and/or reference samples].&lt;/p&gt; &lt;p&gt;2: Probably Good Value: Data value that is probably consistent with real phenomena, but this is unconfirmed or data value forming part of a malfunction that is considered too small to affect the overall quality of the data object of which it is a part. [Used when no replicates or reference samples were available to further verify the quality of the data].&lt;/p&gt; &lt;p&gt;3: Probably Bad Value: Data value recognized as unusual during quality control that forms part of a feature that is probably inconsistent with real phenomena. [Not used].&lt;/p&gt; &lt;p&gt;4: Bad Value: An obviously erroneous data value. [Not used].&lt;/p&gt; &lt;p&gt;5: Changed Value: Data value adjusted during quality control. Best practice strongly recommends that the value before the change be preserved in the data or its accompanying metadata. [Not used].&lt;/p&gt; &lt;p&gt;6: Value Below Detection Limit: The level of the measured phenomenon was less than the limit of detection (LOD) for the method employed to measure it. The accompanying value is the detection limit for the technique or zero if that value is unknown. [Values below detection are reported as 0.00 µM for macronutrients].&lt;/p&gt; &lt;p&gt;7: Value in Excess: The level of the measured phenomenon was too large to be quantified by the technique employed to measure it. The accompanying value is the measurement limit for the technique. [Not used].&lt;/p&gt; &lt;p&gt;8: Interpolated Value: This value has been derived by interpolation from other values in the data object. [Not used].&lt;/p&gt; &lt;p&gt;9: Missing Value: The data value is missing. Any accompanying value will be a magic number representing absent data [When sample was not collected the notation ‘na’ for ‘not applicable’ was used; when sample was collected but there is no result for this parameter, the notation ‘nda’ for ‘no data available’ was used].&lt;/p&gt; &lt;p&gt;A: Value Phenomenon Uncertain: There is uncertainty in the description of the measured phenomenon associated with the value such as chemical species or biological entity. [Not used]&lt;/p&gt; Specified by the Principal Investigator(s) - Imported original file &quot;BCO-DMO_FeOA_FieldData_submit241122.xlsx&quot; into the BCO-DMO system. - Renamed fields to comply with BCO-DMO naming conventions. - Converted all date fields to YYYY-MM-DD format. - Created date-time columns in ISO 8601 for the IN and OUT UTC date-time columns; removed original date-time fields. - Saved the final file as &quot;950296_v1_feoa_profile_data.csv&quot;. Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact BioAnalytical Systems Inc. controlled growth mercury electrode BioAnalytical Systems Inc. controlled growth mercury electrode PI Supplied Instrument Name: BioAnalytical Systems Inc. controlled growth mercury electrode PI Supplied Instrument Description:BioAnalytical Systems Inc. controlled growth mercury electrode and Epsilon Eclipse voltametric analyzer were used for the labile dissolved nickel concentration measurements. Instrument Name: BASi Controlled Growth Mercury Electrode Instrument Short Name:BASI CGME Instrument Description: Bioanalytical Systems (BASi) Mercury drop electrodes are generated by the BASi Controlled Growth Mercury Electrode (CGME) in three modes: DME (Dropping Mercury Electrode) - mercury is allowed to flow freely from the reservoir down the capillary and so the growth of the mercury drop and its lifetime is controlled by gravity. (The optional 100 um capillary is recommended for this mode.) SMDE (Static Mercury Drop Electrode) - the drop size is determined by the length of time for which the fast-response capillary valve is opened, and the drop is dislodged by a drop knocker. The dispense/knock timing is microprocessor-controlled and is typically coordinated with the potential pulse or square-wave waveform. This mode can also used to generate the Hanging Mercury Drop Electrode required for stripping experiments. CGME (Controlled Growth Mercury Electrode) - the mercury drop is grown by a series of pulses that open the capillary valve. The number of pulses, their duration, and their frequency can be varied by PC control, providing great flexibility in both the drop size and its rate of growth. This CGME mode can be used for both polarographic and stripping experiments. http://www.basinc.com/products/ec/cgme.php Epsilon Eclipse voltametric analyzer Epsilon Eclipse voltametric analyzer PI Supplied Instrument Name: Epsilon Eclipse voltametric analyzer PI Supplied Instrument Description:BioAnalytical Systems Inc. controlled growth mercury electrode and Epsilon Eclipse voltametric analyzer were used for the labile dissolved nickel concentration measurements. Instrument Name: BASi EC-epsilon 2 Autoanalyzer Instrument Short Name:BASi EC-epsilon 2 Instrument Description: The Bioanalytical Systems EC epsilon is a family of potentiostat/galvanostats for electrochemistry. The most basic epsilon instrument can be used for standard techniques, as well as chronopotentiometry for materials characterization (e.g., characterization of transition metal complexes by cyclic voltammetry and controlled potential electrolysis, or of biosensors by cyclic voltammetry and constant potential amperometry). Pulse, square wave, and stripping techniques can be added by a software upgrade, and a second channel can be added by a hardware upgrade. ThermoScientific Element II ThermoScientific Element II PI Supplied Instrument Name: ThermoScientific Element II PI Supplied Instrument Description:ThermoScientific Element II high-resolution inductively coupled plasma mass spectrometer was used to measure dissolved metal concentrations. Instrument Name: Inductively Coupled Plasma Mass Spectrometer Instrument Short Name:ICP Mass Spec Instrument Description: An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB15/ Niskin bottle samplers Niskin bottle samplers PI Supplied Instrument Name: Niskin bottle samplers PI Supplied Instrument Description:OceanTest Equipment Inc. modified 12-L x-Niskin bottle samplers were used with the rosette system for trace metal clean seawater collection from depth profiles. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/ QuAAtro39 AutoAnalyzer QuAAtro39 AutoAnalyzer PI Supplied Instrument Name: QuAAtro39 AutoAnalyzer PI Supplied Instrument Description:QuAAtro39 AutoAnalyzer (SEAL Analytical) was used to measure macronutrient concentrations in seawater samples. Instrument Name: Nutrient Autoanalyzer Instrument Short Name:Nutrient Autoanalyzer Instrument Description: Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/ Elemental Scientific seaFAST-pico system Elemental Scientific seaFAST-pico system PI Supplied Instrument Name: Elemental Scientific seaFAST-pico system PI Supplied Instrument Description:Elemental Scientific seaFAST-pico system was used to preconcentrate dissolved trace metals from project samples for the ICPMS analyses. Instrument Name: SeaFAST Automated Preconcentration System Instrument Short Name: Instrument Description: The seaFAST is an automated sample introduction system for analysis of seawater and other high matrix samples for analyses by ICPMS (Inductively Coupled Plasma Mass Spectrometry). Ocean Optics USB4000 fiber optic spectrometer Ocean Optics USB4000 fiber optic spectrometer PI Supplied Instrument Name: Ocean Optics USB4000 fiber optic spectrometer PI Supplied Instrument Description:Ocean Optics USB4000 fiber optic spectrometer for total alkalinity and pH measurements. Instrument Name: Spectrometer Instrument Short Name:Spectrometer Instrument Description: A spectrometer is an optical instrument used to measure properties of light over a specific portion of the electromagnetic spectrum. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0460/ Towfish Towfish PI Supplied Instrument Name: Towfish PI Supplied Instrument Description:Seawater samples were collected with a custom surface sampling system, "towfish" (Mellett and Buck 2020), comprised of acid-cleaned Bev-A-Line-IV tubing and an Almatec Double PTFE Diaphragm Pump. Instrument Name: towed unmanned submersible Instrument Short Name:towed unmanned submersible Instrument Description: A vehicle towed by rigid cable through the water column at fixed or varying depth with no propulsion and no human operator (e.g. Towfish, Scanfish, UOR, SeaSoar). Turner AU10 fluorometer Turner AU10 fluorometer PI Supplied Instrument Name: Turner AU10 fluorometer PI Supplied Instrument Description:Turner AU10 fluorometer was used to measure chlorophyll a fluorescence. Instrument Name: Turner Designs Fluorometer-10 Instrument Short Name:Turner Fluorometer-10 Instrument Description: The Turner Designs Model 10 fluorometer (manufactured by Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA) is used to measure Chlorophyll fluorescence. No information could be found for this specific model. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/ Cruise: SKQ202209S SKQ202209S R/V Sikuliaq Community Standard Description International Council for the Exploration of the Sea R/V Sikuliaq vessel SKQ202209S Mark L. Wells University of Maine R/V Sikuliaq Community Standard Description International Council for the Exploration of the Sea R/V Sikuliaq vessel