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            <gco:CharacterString>Cite this dataset as: Kranz, S. A. (2025) Water column inorganic nutrient concentration and nitrate+nitrite d15N and d18O measurements from R/V Roger Revelle cruise RR2201 in the Argo Basin in the Indian Ocean from February to March of 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-02-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.952591.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Dataset Description: &amp;lt;p&amp;gt;See the section &amp;quot;Related Datasets&amp;quot; on this page for related CTD downcast and bottle data mentioned in the metadata for this dataset.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The RR2201 cruise report can be found at &amp;lt;a href=&amp;quot;http://hdl.handle.net/1834/43464&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;http://hdl.handle.net/1834/43464&amp;lt;/a&amp;gt;.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;Water column samples were collected by Niskin bottle on a CTD rosette (“CTD profile”) during 4 pseudo-Lagrangian cycles (Cycle 1 to Cycle 4)and via Go-flo bottles during a subsequent transect (Argo-stations). All samples were filtered into pre-washed HDPE bottles using a&amp;amp;nbsp; 0.2 µm (Pall Acro Pack) filter capsule. Nutrient bottles were taken in duplicates for analysis of inorganic nutrients ((A-sample) and NO3-isotopes (B-sample). All samples were frozen at -20 °C until analysis.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Inorganic nutrients (NO3, NO2, POx, NH4, Si) were measured through UCSD/Scripps. Nutrient analyses are performed on a Seal Analytical continuous-flow AutoAnalyzer 3 (AA3); https://scripps.ucsd.edu/ships/shipboard-technical-support/odf/chemistry-services/nutrients. The detection limits for the methods/instrumentation are (in micro moles/liter): NO3+NO2 = 0.02; PO4 = 0.02; Sil = 0.5; NO2 = 0.02; NH4 = 0.1&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;NO3 isotope samples were prepared by thawing the 'to-be-analyzed' samples and removing any nitrite using the method described by Granger and Siegman 2009. Nitrate was measured prior and after acidification and refrozen for shipping. The analysis was performed at the UC-Davis Isotope Facility using the &amp;quot;NO3 Analysis by Bacteria Denitrification&amp;quot; - https://stableisotopefacility.ucdavis.edu/nitrate-no3-water.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Accepted Precision: 0.4 ‰ for&amp;amp;nbsp;&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N;&amp;amp;nbsp; &amp;amp;nbsp; 0.5 ‰ for&amp;amp;nbsp;&amp;lt;sup&amp;gt;18&amp;lt;/sup&amp;gt;O&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/819487.rdf" xlink:title="OCE-1851558" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1851558 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1851558</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/835014.rdf" xlink:title="OCE-1851347" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1851347 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1851347</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/907810.rdf" xlink:title="OCE-1851381" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1851381 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1851381</gmx:Anchor>
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The small area between NW Australia and Indonesia in the eastern Indian Ocean (IO) is the only known spawning ground of Southern Bluefin Tuna (SBT), a critically endangered top marine predator. Adult SBT migrate thousands of miles each year from high latitude feeding areas to lay their eggs in these tropical waters, where food concentrations on average are below levels that can support optimal feeding and growth of their larvae. Many critical aspects of this habitat are poorly known, such as the main source of nitrogen nutrient that sustains system productivity, how the planktonic food web operates to produce the unusual types of zooplankton prey that tuna larvae prefer, and how environmental differences in habitat quality associated with ocean fronts and eddies might be utilized by adult spawning tuna to give their larvae a greater chance for rapid growth and survival success. This project investigates these questions on a 38-day expedition in early 2021, during the peak time of SBT spawning. This project is a US contribution to the 2nd International Indian Ocean Expedition (IIOE-2) that advances understanding of biogeochemical and ecological dynamics in the poorly studied eastern IO. This is the first detailed study of nitrogen and carbon cycling in the region linking Pacific and IO waters. The shared dietary preferences of SBT larvae with those of other large tuna and billfish species may also make the insights gained broadly applicable to understanding larval recruitment issues for top consumers in other marine ecosystems. New information from the study will enhance international management efforts for SBT. The shared larval dietary preferences of large tuna and billfish species may also extend the insights gained broadly to many other marine top consumers, including Atlantic bluefin tuna that spawn in US waters of the Gulf of Mexico. The end-to-end study approach, highlights connections among physical environmental variability, biogeochemistry, and plankton food webs leading to charismatic and economically valuable fish production, is the theme for developing educational tools and modules through the &quot;scientists-in-the-schools&quot; program of the Center for Ocean-Atmospheric Prediction Studies at Florida State University, through a program for enhancing STEM learning pathways for underrepresented students in Hawaii, and through public outreach products for display at the Birch Aquarium in San Diego. The study also aims to support an immersive field experience to introduce talented high school students to marine research, with the goal of developing a sustainable marine-related educational program for underrepresented students in rural northwestern Florida.&lt;/p&gt;
&lt;p&gt;Southern Bluefin Tuna (SBT) migrate long distances from high-latitude feeding grounds to spawn exclusively in a small oligotrophic area of the tropical eastern Indian Ocean (IO) that is rich in mesoscale structures, driven by complex currents and seasonally reversing monsoonal winds. To survive, SBT larvae must feed and grow rapidly under environmental conditions that challenge conventional understanding of food-web structure and functional relationships in poor open-ocean systems. The preferred prey of SBT larvae, cladocerans and Corycaeidae copepods, are poorly studied and have widely different implications for trophic transfer efficiencies to larvae. Differences in nitrogen sources - N fixation vs deep nitrate of Pacific origin - to sustain new production in the region also has implications for conditions that may select for prey types (notably cladocerans) that enhance transfer efficiency and growth rates of SBT larvae. The relative importance of these N sources for the IO ecosystem may affect SBT resiliency to projected increased ocean stratification. This research expedition investigates how mesoscale variability in new production, food-web structure and trophic fluxes affects feeding and growth conditions for SBT larvae. Sampling across mesoscale features tests hypothesized relationships linking variability in SBT larval feeding and prey preferences (gut contents), growth rates (otolith analyses) and trophic positions (TP) to the environmental conditions of waters selected by adult spawners. Trophic Positions of larvae and their prey are determined using Compound-Specific Isotope Analyses of Amino Acids (CSIA-AA). Lagrangian experiments investigate underlying process rates and relationships through measurements of water-column 14C productivity, N2 fixation, 15NO3- uptake and nitrification; community biomass and composition (flow cytometry, pigments, microscopy, in situ imaging, genetic analyses); and trophic fluxes through micro- and mesozooplankton grazing, remineralization and export. Biogeochemical and food web elements of the study are linked by CSIA-AA (N source, TP), 15N-constrained budgets and modeling. The project elements comprise an end-to-end coupled biogeochemistry-trophic study as has not been done previously for any pelagic ecosystem.&lt;/p&gt;
&lt;p&gt;This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.&lt;/p&gt;</gco:CharacterString>
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&amp;lt;p&amp;gt;Inorganic nutrients (NO3, NO2, POx, NH4, Si) were measured through UCSD/Scripps. Nutrient analyses are performed on a Seal Analytical continuous-flow AutoAnalyzer 3 (AA3); https://scripps.ucsd.edu/ships/shipboard-technical-support/odf/chemistry-services/nutrients. The detection limits for the methods/instrumentation are (in micro moles/liter): NO3+NO2 = 0.02; PO4 = 0.02; Sil = 0.5; NO2 = 0.02; NH4 = 0.1&amp;lt;/p&amp;gt;

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Missing Data Identifiers:
* In the BCO-DMO data system missing data identifiers are displayed according to the format of data you access. For example, in csv files it will be blank (null) values. In Matlab .mat files it will be NaN values. When viewing data online at BCO-DMO, the missing value will be shown as blank (null) values.

* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores.  Can not start with a number]

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* GPS datetime with timezone (UTC) column added in ISO 8601 format &amp;quot;GPS_DateTime_UTC&amp;quot;.  Since &amp;quot;date&amp;quot; column (TZ undocumented) column did not match the UTC date (compared to event id), the GPS_DateTime_UTC column relied GPS time and the date event number not the &amp;quot;date&amp;quot; column. BCO-DMO data manager will reach out to submitter to find out what time zone &amp;quot;date&amp;quot; column was recorded in and add that information to the Parameters section if it can be found.

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