http://lod.bco-dmo.org/id/dataset/953833
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-02-18
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Mussel respiration data from experiments with mussels sourced in WA conducted at University of Washington Friday Harbor Laboratories, Friday Harbor, WA from 2020 to 2023
2025-02-18
publication
2025-02-18
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-03-24
publication
https://doi.org/10.26008/1912/bco-dmo.953833.1
Michael T. Nishizaki
Carleton College
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Nishizaki, M. T., Delgado, A., Hawadle, A., Brown, R., Woodruff, E., Birk, K. (2025) Mussel respiration data from experiments with mussels sourced in WA conducted at University of Washington Friday Harbor Laboratories, Friday Harbor, WA from 2020 to 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-02-18 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.953833.1 [access date]
Methods and Sampling: <p><em>M. californianus</em>&nbsp;Conrad, 1837 were obtained from Cattle Point on San Juan Island, WA (48°26'59.6811"N,&nbsp;122°57'51.9358"W).&nbsp;<em>M. trossulus</em>&nbsp;and&nbsp;<em>M. galloprovincialis</em>&nbsp;were obtained from a commercial supplier (Penn Cove Shellfish, Coupeville, WA, USA;&nbsp;48°13'06.5579"&nbsp;N, 122°42'25.4331"W).&nbsp;The mussels were maintained in seatables (66 × 135 × 32 cm) with a constant flow of seawater at the Friday Harbor Laboratories (48°32'45.7249" N, 123°00'46.9262"W). Mussels were fed twice daily with a total of approximately 10 mL of Shellfish Diet 1800 TM (Reed Mariculture, Campbell, CA, USA).</p>
<p>Aquatic respiration rates were measured&nbsp;in a closed, recirculating flow chamber of 1-liter capacity. &nbsp;The transparent acrylic test chamber (15 cm × 5 cm × 5 cm, L × W × H) was connected to a submersible pump (360 GPH, Model 25D, Rule Industries, Gloucester, MA, USA) via PVC pipe (25.4 mm i.d.) and custom machined high-density polyethylene connector fittings. The water-tight test chamber was filled with artificial seawater (32 PSU; Instant Ocean; Spectrum Brands, Blacksburg, VA) and submersed in a temperature-controlled water bath (50 L). The water bath's temperature was maintained using a programmable, recirculating water heater/chiller (±0.005°C; AP07R-20-A11B, Polyscience, Niles, IL, USA).&nbsp; Trials were run at water velocities were estimated along the centerline of the testing chamber by tracking the displacement of glass microbeads at each flow setting (mean particle diameter 9 μm, density 2.0 g cm<sup>−3</sup>; Potters Industries, Malvern, PA, USA). &nbsp;<br />
<!--[endif]--></p>
<p>Oxygen concentrations were measured with non-intrusive fiber-optic fluorescence-based optodes and sensor spots on the inner surface of the chamber (FireSting Pro; OXSP5 and&nbsp;TPSP5&nbsp;spots, Pyroscience, Aachen, DEU).&nbsp;Samples were recorded at a rate of 1 Hz and drift of the O2 probe was negligible (e.g., &lt;0.1% over 2 h at 20°C).</p>
<p>Each trial involved positioning individual mussels along the centerline of the chamber and subsequently measuring the organism's oxygen usage at stable temperatures of&nbsp;5, 11, 17, 23, and 29°C. Trials were run for 2 h, ensuring that a stable rate of decline could be identified. After each trial, all soft tissues were dried for 72 hours at 60°C (Lindeberg/Blue M Vacuum Oven; ThermoScientific Inc., Waltham, MA, USA), and weighed using an analytical balance (0.001 g; PA153 Pioneer Analytical Balance; Ohaus Corp., Pine Brook, NJ, USA). &nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2050129 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2050129
completed
Michael T. Nishizaki
Carleton College
507-222-4413
Biology Department One N. College Street
Northfield
MN
55057
United States
mikenish@carleton.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Year
Species
Temperature
Velocity
Dry_weight
Respiration
Lindeberg/Blue M Vacuum Oven; ThermoScientific Inc., Waltham, MA, USA
recirculating water heater/chiller (±0.005°C; AP07R-20-A11B, Polyscience, Niles, IL, USA)
non-intrusive fiber-optic fluorescence-based optodes and sensor spots (FireSting Pro; OXSP5 and TPSP5 spots, Pyroscience, Aachen, DEU)
O2 sensors (Pyroscience, Aachen, DEU)
submersible pump (360 GPH, Model 25D, Rule Industries, Gloucester, MA, USA)
PA153 Pioneer Analytical Balance; Ohaus Corp., Pine Brook, NJ, USA
theme
None, User defined
year
verbatim identification
water temperature at measurement depth
Absolute current speed
dry_wgt
respiration rate
featureType
BCO-DMO Standard Parameters
Drying Oven
Immersion heater
Optode
Oxygen Sensor
Pump
scale or balance
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Microscale interactions of foundation species with their fluid environment: biological feedbacks alter ecological interactions of mussels
https://osprey.bco-dmo.org/project/872978
Collaborative Research: Microscale interactions of foundation species with their fluid environment: biological feedbacks alter ecological interactions of mussels
<p><em>NSF Award Abstract:</em><br />
The project investigates how the metabolic activity of dense aggregations of marine organisms alter the water chemistry of their interstitial spaces, and how these microscale alterations feedback to affect the organisms’ interactions in coastal ecosystems. The research team focuses on bivalve mussels, foundation species that form dense ‘beds’ typically known for facilitating other species by ameliorating harsh flow conditions. This ability can become a liability, however, if flow is not sufficient to flush the interstitial spaces and steep, metabolically-driven concentration gradients develop. The research evaluates whether corrosive chemical microclimates (such as low oxygen or low pH) are most extreme in low flow, high temperature conditions, especially for dense aggregations of mussels with large biomass and/or high respiration rates, and if they negatively impact mussel beds and the diverse biological communities they support. The research addresses a global societal concern, the impact of anthropogenic climate change on coastal marine ecosystems, and has potential applications to aquaculture and biofouling industries by informing adaptation strategies to “future-proof” mussel farms in the face of climate change and improved antifouling practices for ships, moorings, and industrial cooling systems. The project forges new collaborations with investigators from three campuses and integrates research and education through interdisciplinary training of a diverse group of graduate, undergraduate and high school students. STEM education and environmental stewardship is promoted by the development of a K-12 level science curriculum module and a hand’s-on public exhibit of bivalve biology at a local shellfish farm. Research findings are disseminated in a variety of forums, including peer-reviewed scientific publications and research presentations at regional, national and international meetings.</p>
<p>The research team develops a framework that links environmental conditions measured at a coarse scale (100m-100km; e.g., most environmental observatories) and ecological processes at the organismal scale (1 cm – 10 m). Specifically, the project investigates how aggregations of foundation species impact flow through interstitial spaces, and how this ultimately impacts water chemistry immediately adjacent to the organisms. The research focuses on mytilid mussels, with the expectation that the aggregation alters the flow and chemical transport in two ways, one by creating a physical resistance, which reduces the exchange, and the other by enhancing the exchange due to their incurrent/excurrent pumping. These metabolically-driven feedbacks are expected to be strongest in densely packed, high biomass aggregations and under certain ambient environmental conditions, namely low flow and elevated temperature, and can lead to a range of negative ecological impacts that could not be predicted directly from coarse scale measures of ambient seawater chemistry or temperature. The team develops computational fluid dynamic (CFD) models to predict interstitial flows and concentration gradients of dissolved oxygen and pH within mussel beds. The CFD model incorporates mussel behavior and physiological activity (filtration, gaping, respiration) based on published values as well as new empirical work. Model predictions are compared to flow and concentration gradients measured in mussel aggregations in the laboratory and field. Finally, the team conducts several short-term experiments to quantify some of the potential negative ecological impacts of corrosive interstitial water chemistry on mussel aggregations, such as reduced growth, increased dislodgement, increased predation risk, and reduced biodiversity. Because the model is based on fluid dynamic principles and functional traits, the framework is readily adaptable to other species that form dense assemblages, thereby providing a useful tool for predicting the ability of foundation species to persist and provide desirable ecosystem services under current and future multidimensional climate scenarios.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Microscale Mussels
largerWorkCitation
project
eng; USA
oceans
-123.013046
-122.7070647
48.2184883
48.545998
2021-06-14
2023-09-01
University of Washington Friday Harbor Laboratories
0
BCO-DMO catalogue of parameters from Mussel respiration data from experiments with mussels sourced in WA conducted at University of Washington Friday Harbor Laboratories, Friday Harbor, WA from 2020 to 2023
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/956798.rdf
Name: Year
Units: unitless
Description: <p>Year</p>
http://lod.bco-dmo.org/id/dataset-parameter/954118.rdf
Name: Species
Units: unitless
Description: <p>species names of mussels used in experiments. M_trossulus = Mytilus trossulus;M_gallo = Mytilus galloprovincialis;M_cali = Mytilus californianus (see Supplemental File mussel_collection_information.csv for more information about names and collection information).</p>
http://lod.bco-dmo.org/id/dataset-parameter/954119.rdf
Name: Temperature
Units: degrees Celsius (deg C)
Description: <p>water temperatures in degrees celsius</p>
http://lod.bco-dmo.org/id/dataset-parameter/954120.rdf
Name: Velocity
Units: centimeters per second (cm s-1)
Description: <p>water velocities</p>
http://lod.bco-dmo.org/id/dataset-parameter/956799.rdf
Name: Dry_weight
Units: milligrams (mg)
Description: <p>Dry weight for each mussel (see methodology)</p>
http://lod.bco-dmo.org/id/dataset-parameter/954121.rdf
Name: Respiration
Units: micromoles of O2 per gram per hour (umol O2 g-1 h-1)
Description: <p>respiration rate</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
7864
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/83bfe27e-634c-4287-8378-fcb0a4ef3b08/content
download
https://doi.org/10.26008/1912/bco-dmo.953833.1
download
onLine
dataset
<p><em>M. californianus</em>&nbsp;Conrad, 1837 were obtained from Cattle Point on San Juan Island, WA (48°26'59.6811"N,&nbsp;122°57'51.9358"W).&nbsp;<em>M. trossulus</em>&nbsp;and&nbsp;<em>M. galloprovincialis</em>&nbsp;were obtained from a commercial supplier (Penn Cove Shellfish, Coupeville, WA, USA;&nbsp;48°13'06.5579"&nbsp;N, 122°42'25.4331"W).&nbsp;The mussels were maintained in seatables (66 × 135 × 32 cm) with a constant flow of seawater at the Friday Harbor Laboratories (48°32'45.7249" N, 123°00'46.9262"W). Mussels were fed twice daily with a total of approximately 10 mL of Shellfish Diet 1800 TM (Reed Mariculture, Campbell, CA, USA).</p>
<p>Aquatic respiration rates were measured&nbsp;in a closed, recirculating flow chamber of 1-liter capacity. &nbsp;The transparent acrylic test chamber (15 cm × 5 cm × 5 cm, L × W × H) was connected to a submersible pump (360 GPH, Model 25D, Rule Industries, Gloucester, MA, USA) via PVC pipe (25.4 mm i.d.) and custom machined high-density polyethylene connector fittings. The water-tight test chamber was filled with artificial seawater (32 PSU; Instant Ocean; Spectrum Brands, Blacksburg, VA) and submersed in a temperature-controlled water bath (50 L). The water bath's temperature was maintained using a programmable, recirculating water heater/chiller (±0.005°C; AP07R-20-A11B, Polyscience, Niles, IL, USA).&nbsp; Trials were run at water velocities were estimated along the centerline of the testing chamber by tracking the displacement of glass microbeads at each flow setting (mean particle diameter 9 μm, density 2.0 g cm<sup>−3</sup>; Potters Industries, Malvern, PA, USA). &nbsp;<br />
<!--[endif]--></p>
<p>Oxygen concentrations were measured with non-intrusive fiber-optic fluorescence-based optodes and sensor spots on the inner surface of the chamber (FireSting Pro; OXSP5 and&nbsp;TPSP5&nbsp;spots, Pyroscience, Aachen, DEU).&nbsp;Samples were recorded at a rate of 1 Hz and drift of the O2 probe was negligible (e.g., &lt;0.1% over 2 h at 20°C).</p>
<p>Each trial involved positioning individual mussels along the centerline of the chamber and subsequently measuring the organism's oxygen usage at stable temperatures of&nbsp;5, 11, 17, 23, and 29°C. Trials were run for 2 h, ensuring that a stable rate of decline could be identified. After each trial, all soft tissues were dried for 72 hours at 60°C (Lindeberg/Blue M Vacuum Oven; ThermoScientific Inc., Waltham, MA, USA), and weighed using an analytical balance (0.001 g; PA153 Pioneer Analytical Balance; Ohaus Corp., Pine Brook, NJ, USA). &nbsp;</p>
Specified by the Principal Investigator(s)
<p>All analyses were conducted in R (ver. 4.4.2; RStudio 2024.12.0 (build 467). The R package&nbsp;respR&nbsp;was used to analyze the respiration data (Harianto, 2019). Respiration rates were standardized by the dry biomass of the mussels.</p>
Specified by the Principal Investigator(s)
* Data table within submitted file "Mussel_respiration_data.csv" was imported into the BCO-DMO data system for this dataset. Table will appear as Data File: 953833_v1_mussel-respiration-rates.csv (along with other download format options).
* Metadata extracted from methodology and added to table mussel_collection_information.csv including geospaital bounds (in decimal degrees), site information, and a lookup table for the species code used in the dataset matched to the full Scientific Name (authority) and LSID.
* LSIDs added for taxonomic names used in the metadata using the world register of marine species on 2025-02-20.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Lindeberg/Blue M Vacuum Oven; ThermoScientific Inc., Waltham, MA, USA
Lindeberg/Blue M Vacuum Oven; ThermoScientific Inc., Waltham, MA, USA
PI Supplied Instrument Name: Lindeberg/Blue M Vacuum Oven; ThermoScientific Inc., Waltham, MA, USA Instrument Name: Drying Oven Instrument Short Name: Instrument Description: a heated chamber for drying
recirculating water heater/chiller (±0.005°C; AP07R-20-A11B, Polyscience, Niles, IL, USA)
recirculating water heater/chiller (±0.005°C; AP07R-20-A11B, Polyscience, Niles, IL, USA)
PI Supplied Instrument Name: recirculating water heater/chiller (±0.005°C; AP07R-20-A11B, Polyscience, Niles, IL, USA) PI Supplied Instrument Description:The water bath's temperature was maintained using a programmable, recirculating water heater/chiller (±0.005°C; AP07R-20-A11B, Polyscience, Niles, IL, USA). Instrument Name: Immersion heater Instrument Short Name:Immersion heater Instrument Description: Submersible heating element for water tanks and aquaria.
non-intrusive fiber-optic fluorescence-based optodes and sensor spots (FireSting Pro; OXSP5 and TPSP5 spots, Pyroscience, Aachen, DEU)
non-intrusive fiber-optic fluorescence-based optodes and sensor spots (FireSting Pro; OXSP5 and TPSP5 spots, Pyroscience, Aachen, DEU)
PI Supplied Instrument Name: non-intrusive fiber-optic fluorescence-based optodes and sensor spots (FireSting Pro; OXSP5 and TPSP5 spots, Pyroscience, Aachen, DEU) Instrument Name: Optode Instrument Short Name: Instrument Description: An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.
O2 sensors (Pyroscience, Aachen, DEU)
O2 sensors (Pyroscience, Aachen, DEU)
PI Supplied Instrument Name: O2 sensors (Pyroscience, Aachen, DEU) PI Supplied Instrument Description:Oxygen concentrations were measured with non-intrusive fiber-optic O2 sensors (Pyroscience, Aachen, DEU). Samples were recorded at a rate of 1 Hz and drift of the O2 probe was negligible (e.g., Instrument Name: Oxygen Sensor Instrument Short Name:Dissolved Oxygen Sensor Instrument Description: An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed
submersible pump (360 GPH, Model 25D, Rule Industries, Gloucester, MA, USA)
submersible pump (360 GPH, Model 25D, Rule Industries, Gloucester, MA, USA)
PI Supplied Instrument Name: submersible pump (360 GPH, Model 25D, Rule Industries, Gloucester, MA, USA) Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps
PA153 Pioneer Analytical Balance; Ohaus Corp., Pine Brook, NJ, USA
PA153 Pioneer Analytical Balance; Ohaus Corp., Pine Brook, NJ, USA
PI Supplied Instrument Name: PA153 Pioneer Analytical Balance; Ohaus Corp., Pine Brook, NJ, USA PI Supplied Instrument Description:analytical balance (0.001 g; PA153 Pioneer Analytical Balance; Ohaus Corp., Pine Brook, NJ, USA) Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/