http://lod.bco-dmo.org/id/dataset/954189
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-02-21
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Microplankton microscopy and biovolume analysis from Lugol's samples collected in Resurrection Bay, AK from January to March of 2023
2025-02-21
publication
2025-02-21
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-04-10
publication
https://doi.org/10.26008/1912/bco-dmo.954189.1
Petra H. Lenz
University of Hawaiʻi at Mānoa
principalInvestigator
Lauren N Block
University of Hawaiʻi at Mānoa
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Block, L. N., Lenz, P. H. (2025) Microplankton microscopy and biovolume analysis from Lugol's samples collected in Resurrection Bay, AK from January to March of 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-02-21 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.954189.1 [access date]
Methods and Sampling: <p>Microzooplankton samples were collected at 3 depths (50, 150, and 280 m) by gently draining 250 mL of seawater from the Niskin bottle with a silicon tube to avoid bubbling. Samples were then preserved with acid Lugol’s solution to a final concentration of 5% (Strom et al., 2019). Glass was added to saturate silica and prevent dissolution of diatoms. Samples were gently mixed and 100 mL subsamples were settled onto microscope slides. Diatoms, dinoflagellates, and ciliates were identified to lowest taxonomic classification, enumerated, and imaged under an inverted microscope. Linear length and widths of cells were measured for a subset of 5 cells (or maximum number) per sample per taxon using ImageJ. Biovolume was estimated using the median linear measurements per taxon and biovolume was calculated using the volumetric equations most closely matching the cell shape (Hillebrand et al., 1999). Biovolume was then converted to carbon biomass according to established diatom and protist carbon:volume relationships (Menden-Deuer and Lessard, 2000).<br />
<br />
Day-trips aboard the R/V Nanuq conducted approximately biweekly in Resurrection Bay, AK during January to March, 2023.</p>
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OCE-2222376 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2222376
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OCE-2222592 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2222592
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OCE-2222558 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2222558
completed
Petra H. Lenz
University of Hawaiʻi at Mānoa
808-956-8003
Pacific Biosciences Research Center (PBRC), Bekesy Laboratory of Neurobiology 1993 East-West Road
Honolulu
HI
96822
United States
petra@hawaii.edu
pointOfContact
Lauren N Block
University of Hawaiʻi at Mānoa
lblockln7@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
cruise
date
station
latitude
longitude
depth
sample_volume
group
taxa
cell_count
abundance
cell_biovolume
cell_carbon
biomass
Inverted microscope (Olympus IM)
theme
None, User defined
Cruise identfier
date
station
latitude
longitude
depth
sample_volume
animal group (category)
taxon
count
cell_concentration
biovolume
Elemental carbon (C)
biomass_C
featureType
BCO-DMO Standard Parameters
CTD - profiler
Inverted Microscope
Niskin bottle
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Zooplankton restarts in a high-latitude marine ecosystem: species-specific recruitment and development in early spring
https://osprey.bco-dmo.org/project/908803
Collaborative Research: Zooplankton restarts in a high-latitude marine ecosystem: species-specific recruitment and development in early spring
<p><strong>NSF Award Abstract</strong><br />
Global climate change and associated extreme weather events are increasingly impacting marine communities at all trophic levels and leading to shifts in the timing of life history events. This project is investigating the annual restart of the spring zooplankton community in the Gulf of Alaska in order to determine the timing of species-specific recruitment and growth. Zooplankton are small pelagic animals that are a critical link between microalgae and protozoans and higher levels in the food web including economically important fishes, birds and marine mammals. While their abundances and species composition have been documented over part of the annual cycle between late spring and fall, this project focuses on winter and early spring. The project integrates traditional methods with modern molecular approaches to characterize the diversity, development, feeding and physiology of zooplankton, especially the early developmental stages of copepods (small crustaceans). The goal is to determine which species are there, how many are present and where they are in the water column, and to reveal indicators of their health. Broader impacts include research training for three graduate students and at least four undergraduates in biological oceanography and physiological ecology. Outreach activities are focusing on broadening the public’s understanding of plankton ecology. An illustrated zooplankton guide for the Gulf of Alaska and plankton module for school teachers and students is being produced in collaboration with the Center for Alaskan Coastal Studies. Other plans include sponsorship of nature-drawing workshops on zooplankton and the production of an Art & Science traveling exhibit.</p>
<p>This project is tracking zooplankton population abundances, species composition and developmental stages through the spring restart in a high-latitude fjord in the northern Gulf of Alaska. While the entire zooplankton community is being characterized, the main focus is on the difficult-to-assess early developmental stages of copepods, which dominate the late spring biomass in the region. Three central hypotheses guide the research: 1) high abundances of copepod nauplii are present before any measurable increases in food in surface waters; 2) species diversity increases between winter and spring, with nauplii from large lipid-rich capital-breeding species appearing first, followed by those from income- and hybrid-strategy species and finally nauplii that emerge from dormant eggs; 3) prior to the appearance of food resources, nauplii from capital-breeding species conserve resources by delaying development and entering a state of dormancy in the second and third naupliar stages. The project entails intensive depth-stratified field sampling to characterize the wild community, in combination with laboratory experiments on nauplii to determine their responsiveness to food. The prey are being characterized by measuring chlorophyll a, dietary and prey community DNA sequencing and flow cytometry to establish diversity and abundances. Size-fractionated zooplankton samples are being analyzed using microscopy and community DNA sequencing to ascertain species diversity, developmental stage distribution and abundances. Feeding activity is being measured using dietary DNA sequencing of nauplii followed by comparisons with the prey field. Dormancy in nauplii is being determined by differential gene expression of target genes (RT-qPCR) and high-throughput sequencing of mRNA of individuals (transcriptomics) and community samples (meta-transcriptomics). Short-term and long-term effects of food availability on dormancy, development and growth are being quantified in laboratory experiments. Broader impacts are focused on training of students in interdisciplinary research and state-of-art techniques, and public outreach to introduce plankton ecology to broader audiences.</p>
Zooplankton recruitment
largerWorkCitation
project
eng; USA
oceans
-149.3583
-149.3583
60.025
60.025
2023-01-05
2023-03-24
Sub-arctic marine ecosystem, Gulf of Alaska
0
BCO-DMO catalogue of parameters from Microplankton microscopy and biovolume analysis from Lugol's samples collected in Resurrection Bay, AK from January to March of 2023
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/954410.rdf
Name: cruise
Units: unitless
Description: <p>Cruise ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/954411.rdf
Name: date
Units: unitless
Description: <p>Date (Local time) in ISO 8601 format yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/954412.rdf
Name: station
Units: unitless
Description: <p>Station ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/954413.rdf
Name: latitude
Units: Decimal Degrees North
Description: <p>Station latitude, north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/954414.rdf
Name: longitude
Units: Decimal Degrees East
Description: <p>Station latitude, west is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/954415.rdf
Name: depth
Units: Meters (m)
Description: <p>Target water collection depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/954416.rdf
Name: sample_volume
Units: Milliliters (ml)
Description: <p>Volume of sample processed for microscopy</p>
http://lod.bco-dmo.org/id/dataset-parameter/954417.rdf
Name: group
Units: unitless
Description: <p>Broad taxonomic grouping (Diatom, Dinoflagellate, Cilliate)</p>
http://lod.bco-dmo.org/id/dataset-parameter/954418.rdf
Name: taxa
Units: unitless
Description: <p>Taxonomic identification to lowest taxonomic level</p>
http://lod.bco-dmo.org/id/dataset-parameter/954419.rdf
Name: cell_count
Units: Integer
Description: <p>Number of cells observed in the sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/954420.rdf
Name: abundance
Units: Cells per milliliter (#/ml)
Description: <p>Number of cells per ml of sample per taxa</p>
http://lod.bco-dmo.org/id/dataset-parameter/954421.rdf
Name: cell_biovolume
Units: Cubic micrometers (µg^3)
Description: <p>Estimated biovolume per cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/954422.rdf
Name: cell_carbon
Units: Picograms carbon (pg)
Description: <p>Estimated carbon per cell based on biovolume estimate</p>
http://lod.bco-dmo.org/id/dataset-parameter/954423.rdf
Name: biomass
Units: Picograms carbon per milliliter (pg/ml)
Description: <p>Biomass per mL of sample per taxa</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
27380
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/7b3d4945-20d8-4e06-9a64-7015046b356e/content
download
https://doi.org/10.26008/1912/bco-dmo.954189.1
download
onLine
dataset
<p>Microzooplankton samples were collected at 3 depths (50, 150, and 280 m) by gently draining 250 mL of seawater from the Niskin bottle with a silicon tube to avoid bubbling. Samples were then preserved with acid Lugol’s solution to a final concentration of 5% (Strom et al., 2019). Glass was added to saturate silica and prevent dissolution of diatoms. Samples were gently mixed and 100 mL subsamples were settled onto microscope slides. Diatoms, dinoflagellates, and ciliates were identified to lowest taxonomic classification, enumerated, and imaged under an inverted microscope. Linear length and widths of cells were measured for a subset of 5 cells (or maximum number) per sample per taxon using ImageJ. Biovolume was estimated using the median linear measurements per taxon and biovolume was calculated using the volumetric equations most closely matching the cell shape (Hillebrand et al., 1999). Biovolume was then converted to carbon biomass according to established diatom and protist carbon:volume relationships (Menden-Deuer and Lessard, 2000).<br />
<br />
Day-trips aboard the R/V Nanuq conducted approximately biweekly in Resurrection Bay, AK during January to March, 2023.</p>
Specified by the Principal Investigator(s)
<p>For the microscopy, biovolume was estimated using the median linear measurements per taxon and biovolume was calculated using the volumetric equations most closely matching the cell shape (Hillebrand et al., 1999). Biovolume was then converted to carbon biomass according to established diatom and protist carbon:volume relationships (Menden-Deuer and Lessard, 2000).</p>
Specified by the Principal Investigator(s)
* Sheet 1 of submitted file "Sheet 1-1-NaupProj2023_Lugols_BCODMO.csv" was imported into the BCO-DMO data system for this dataset. Table will appear as Data File: 954189_v1_microplankton-microscopy.csv (along with other download format options).
* A unique list of taxonomic names used in the data table was matched with identifiers using the World Register of Marine Species (WoRMS, marinspecies.org) taxa match tool on 2025-02-24. The resulting species list was attached as a supplemental file.
* "Tintinnid" was matched to "Tintinnidae" the formal taxon representation of the name. urn:lsid:marinespecies.org:taxname:183533
* The incorrect phonetic spelling of "Dytilum" was corrected to "Ditylum" (urn:lsid:marinespecies.org:taxname:149022) in the data table and subsequent supplemental file.
* "Navicula" and "Nitzschia" returned ambiguous name match results however, each only had one matching result that was a diatom as described by the column "group" to the appropriate LSIDs could be assigned in this dataset. The other possible matches were in Mollusca and Platyhelminthes.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:SBE55 rosette with 6 4-L Niskin Bottles Instrument Name: CTD - profiler Instrument Short Name: Instrument Description: The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.
This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
Inverted microscope (Olympus IM)
Inverted microscope (Olympus IM)
PI Supplied Instrument Name: Inverted microscope (Olympus IM) Instrument Name: Inverted Microscope Instrument Short Name: Instrument Description: An inverted microscope is a microscope with its light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up. It was invented in 1850 by J. Lawrence Smith, a faculty member of Tulane University (then named the Medical College of Louisiana).
Inverted microscopes are useful for observing living cells or organisms at the bottom of a large container (e.g. a tissue culture flask) under more natural conditions than on a glass slide, as is the case with a conventional microscope. Inverted microscopes are also used in micromanipulation applications where space above the specimen is required for manipulator mechanisms and the microtools they hold, and in metallurgical applications where polished samples can be placed on top of the stage and viewed from underneath using reflecting objectives.
The stage on an inverted microscope is usually fixed, and focus is adjusted by moving the objective lens along a vertical axis to bring it closer to or further from the specimen. The focus mechanism typically has a dual concentric knob for coarse and fine adjustment. Depending on the size of the microscope, four to six objective lenses of different magnifications may be fitted to a rotating turret known as a nosepiece. These microscopes may also be fitted with accessories for fitting still and video cameras, fluorescence illumination, confocal scanning and many other applications. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
PI Supplied Instrument Name: Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/