http://lod.bco-dmo.org/id/dataset/954333
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-02-25
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Isotopic data from sponges collected in 2013 and 2014 from reefs in Honduras, Belize, Panama and the Florida Keys.
2025-02-25
publication
2025-02-25
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-05-07
publication
https://doi.org/10.26008/1912/bco-dmo.954333.1
David M. Baker
University of Hong Kong
principalInvestigator
Cole G. Easson
Middle Tennessee State University
principalInvestigator
Christopher J. Freeman
College of Charleston
principalInvestigator
Kenan Matterson
University of Alabama at Birmingham
principalInvestigator
Valerie J. Paul
Smithsonian Marine Station
principalInvestigator
Robert W. Thacker
Stony Brook University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Freeman, C. J., Easson, C. G., Thacker, R. W., Matterson, K., Paul, V. J., Baker, D. M. (2025) Isotopic data from sponges collected in 2013 and 2014 from reefs in Honduras, Belize, Panama and the Florida Keys. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-02-25 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.954333.1 [access date]
Isotope data sponges 2013-2014 Dataset Description: Methods and Sampling: <p>Sponge collection:&nbsp; Sponge species were collected from at least one site within four geographic regions spanning more than 15° of latitude (~1700 km) within the Caribbean Sea. Individual regions included the Bocas del Toro archipelago of Panama, the Miskito Cays of Honduras, the Mesoamerican barrier reef of Belize, and the Florida Keys. At each site, replicate small (3–5 ml) samples of dominant and conspicuous sponge species were collected by SCUBA using a dive knife and placed into individual bags containing seawater for transport back to the laboratory. Sponge samples always included a cross section with both inner and outer tissue regions to standardize collections and sample across the entire body of the sponge. Collections frequently included eight of the ten most dominant Caribbean species&nbsp;and species previously designated as both HMA and LMA sponges. Samples were preserved, processed, and prepared for&nbsp;<em>δ</em>13C and&nbsp;<em>δ</em>15N analysis. Sponges were identified to species and, if necessary, identities were verified via tissue histology and spicule preparations. Replicate subsamples of each sponge species were also preserved in 95% EtOH in 5 ml cryovials and frozen at −20 °C for analyses of microbial community structure.</p>
<p>Stable isotope and chlorophyll&nbsp;<em>a</em>&nbsp;analyses: Stable isotope values (<em>δ</em>13C and&nbsp;<em>δ</em>15N) of bulk sponge tissue serve as a time-integrated record of the sources of carbon and nitrogen assimilated by a holobiont (including activities of both sponge and microbial cells) and any fractionation associated with symbiont or host metabolism or nutrient recycling. Within an individual reef,&nbsp;<em>δ</em>13C and&nbsp;<em>δ</em>15N values of sponge tissue therefore act as a metabolic “fingerprint” that integrates the physiological, metabolic, and ecological differences present across individual sponges.&nbsp;Bulk sponge tissue samples were analyzed in the Stable Isotope Ratio Mass Spectrometry Laboratory at the University of Hong Kong as in. Mean (±SE) precision during analysis was 0.1 (0.001) ‰ and 0.2 (0.03) ‰ for&nbsp;<em>δ</em>13C and&nbsp;<em>δ</em>15N, respectively. Isotope values are expressed in delta (<em>δ</em>) notation in units per mille (‰). Values of the elemental composition (%C, %N, and C:N) of each sample of sponge tissue were also provided. Elemental values provide important information about how biomass-associated pools of carbon and nitrogen vary across sponge species and allowed us to test whether our trends in&nbsp;<em>δ</em>13C and&nbsp;<em>δ</em>15N values were strongly influenced by structural differences in sponge tissue. Photosymbiont abundance (as determined by chlorophyll&nbsp;<em>a</em>&nbsp;[chl&nbsp;<em>a</em>] concentration) was quantified in sponges from sites in Honduras, Panama, and the Florida Keys as in&nbsp;and expressed as μg chl&nbsp;<em>a</em>&nbsp;[g dry sponge tissue]−1.&nbsp;<em>Scopalina ruetzleri</em>&nbsp;samples were not analyzed for chl&nbsp;<em>a</em>&nbsp;because they were too small to provide tissue for both isotope and chl&nbsp;<em>a</em>&nbsp;analyses.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1929293 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1929293
completed
David M. Baker
University of Hong Kong
The Swire Institute of Marine Science Kadoorie Biological Sciences Building, Pokfulam Road
Hong Kong
P.R. China
dmbaker@hku.hk
pointOfContact
Cole G. Easson
Middle Tennessee State University
615-898-5822
1672 Greenland Drive SCI 2044
Murfreesboro
TN
37132
USA
Cole.Easson@mtsu.edu
pointOfContact
Christopher J. Freeman
College of Charleston
817-707-5840
66 George Street Department of Biology
Charleston
SC
29424
USA
freemancj@cofc.edu
pointOfContact
Kenan Matterson
University of Alabama at Birmingham
kenanm@gmail.com
pointOfContact
Valerie J. Paul
Smithsonian Marine Station
772-462-0982
701 Seaway Drive
Fort Pierce
FL
34949
USA
paul@si.edu
pointOfContact
Robert W. Thacker
Stony Brook University
631-632-9949
West 5510 FRK MEL LIB
Stony Brook
NY
11794
USA
robert.thacker@stonybrook.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Species_name
Region_Country
Lat
Lon
Month_and_year_of_collection
Ave_depth_ft
Freeman_Mean_Chla_Dry_wt
HL_Chla
HMA_LMA
Site_Name
Site_Number
Site_ID
Species_Abbreviation
d15N
d13C
Percentage_N
Percentage_C
C_N
Eurovector EA3028 coupled to a Perspective IRMS (Nu Instruments)
theme
None, User defined
species scientific name (binomial)
region
latitude
longitude
date
depth_w
chlorophyll a
abundance
site
d15N
d13C
Nitrogen
Carbon to Nitrogen ratio (C:N)
featureType
BCO-DMO Standard Parameters
Isotope-ratio Mass Spectrometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Investigations into microbially mediated ecological diversification in sponges
https://osprey.bco-dmo.org/project/772220
Collaborative Research: Investigations into microbially mediated ecological diversification in sponges
<p><em>NSF Award Abstract:</em><br />
Coral reefs represent a paradox because, despite their immense productivity and biodiversity, they are found in nutrient-poor habitats that are equivalent to "marine deserts." High biodiversity is often associated with a division of resources that allows many types of organisms to coexist with minimal competition. Indeed, unlike many other organisms on coral reefs, sponges are adapted to efficiently remove bacteria, phytoplankton, and dissolved organic matter from seawater by filter-feeding. Sponges are a dominant component of coral reefs worldwide and in the Caribbean, where their biomass exceeds that of reef-building corals. For almost a quarter century, the success of sponges in the Caribbean has been linked to their filter-feeding ability. However, recent work demonstrated that coexisting sponges on Caribbean reefs host unique communities of bacteria that might allow sponges to access multiple pools of nutrients that are not available to other organisms. In this project, the investigators will test the hypothesis that ecologically dominant sponge species in the Caribbean have unique metabolic strategies that are mediated by their associations with microbes that live within the sponge body. This research will combine manipulative field experiments with a novel combination of modern analytical tools to investigate both filter-feeding by sponge hosts and the metabolic pathways of their microbes. This work will advance our understanding of the ecological and evolutionary forces that have helped shape the species present on Caribbean coral reefs. Additionally, this project will support three early-career investigators and provide training opportunities for graduate and undergraduate students at Nova Southeastern University, Appalachian State University, Stony Brook University, and Smithsonian Marine Station. The investigators will also develop innovative outreach programs that expand existing platforms at their institutions to increase public engagement and scientific literacy.</p>
<p>Marine sponges have been widely successful in their expansion across ecological niches in the Caribbean, with biomass often exceeding that of reef-building corals and high species diversity. However, whether this success is linked to efficient heterotrophic filter-feeding on organic carbon in the water column or to their evolutionary investment in microbial symbionts is yet to be fully elucidated. Microbial symbionts expand the metabolic capabilities of host sponges, supplementing heterotrophic feeding with inorganic carbon and nitrogen, mediating the assimilation of dissolved organic matter, and facilitating recycling of host-derived nitrogen. Despite these benefits, microbial symbiont communities are widely divergent across coexisting sponge species and there is substantial variation in host reliance on symbiont-derived carbon and nitrogen among host sponges; therefore, these associations likely mediate the ecological diversification of coexisting sponge species. The goal of this project is to test this transformative hypothesis by adopting an integrative approach to assess the individual components of holobiont metabolism (i.e., microbial symbionts and sponge host) in ten of the most common sponge species in the Caribbean. The investigators will isolate autotrophic and heterotrophic metabolic pathways and explore potential links between microbial symbiont community composition and the assimilation of particulate and dissolved organic matter (POM and DOM) from seawater. This project will elucidate whether Caribbean sponge species are on similar or divergent evolutionary trajectories, and will provide information that is critical for our understanding of how conditions in the Caribbean basin have shaped the evolution of benthic organisms.</p>
Ecological Diversification in Sponges
largerWorkCitation
project
eng; USA
oceans
-88.113819
-81.442467
9.243206
24.578192
2013-05-01
2014-05-31
Caribbean coast of Panama
0
BCO-DMO catalogue of parameters from Isotopic data from sponges collected in 2013 and 2014 from reefs in Honduras, Belize, Panama and the Florida Keys.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/954381.rdf
Name: Species_name
Units: unitless
Description: <p>Scientific name for sponge species</p>
http://lod.bco-dmo.org/id/dataset-parameter/954382.rdf
Name: Region_Country
Units: unitless
Description: <p>Broad region of collection (by country: Belize, Honduras, Panama, and Florida Keys)</p>
http://lod.bco-dmo.org/id/dataset-parameter/954383.rdf
Name: Lat
Units: decimal
Description: <p>Latitude of collection site</p>
http://lod.bco-dmo.org/id/dataset-parameter/954384.rdf
Name: Lon
Units: decimal
Description: <p>Longitude of collection site</p>
http://lod.bco-dmo.org/id/dataset-parameter/954385.rdf
Name: Month_and_year_of_collection
Units: unitless
Description: <p>Month-year</p>
http://lod.bco-dmo.org/id/dataset-parameter/954386.rdf
Name: Ave_depth_ft
Units: feet
Description: <p>Average water depth at collection site</p>
http://lod.bco-dmo.org/id/dataset-parameter/954387.rdf
Name: Freeman_Mean_Chla_Dry_wt
Units: micrograms of chlorophyll a per gram of sponge tissue
Description: <p>Concentration of chlorophyll a in sponge tissue (as a proxy for photosymbiont abundance)</p>
http://lod.bco-dmo.org/id/dataset-parameter/954388.rdf
Name: HL_Chla
Units: unitless
Description: <p>Discrete chlorophyll a concentration category (high: greater than 125; low: less than 125)</p>
http://lod.bco-dmo.org/id/dataset-parameter/954389.rdf
Name: HMA_LMA
Units: unitless
Description: <p>Overall microbial symbiont abundance (high: HMA or low: LMA)</p>
http://lod.bco-dmo.org/id/dataset-parameter/954390.rdf
Name: Site_Name
Units: unitless
Description: <p>Specific site name within broader region</p>
http://lod.bco-dmo.org/id/dataset-parameter/954391.rdf
Name: Site_Number
Units: unitless
Description: <p>Specific site or dive number within a site if multiple dives were made at one site</p>
http://lod.bco-dmo.org/id/dataset-parameter/954392.rdf
Name: Site_ID
Units: unitless
Description: <p>Site abbreviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/954393.rdf
Name: Species_Abbreviation
Units: unitless
Description: <p>Abbreviation of species name</p>
http://lod.bco-dmo.org/id/dataset-parameter/954394.rdf
Name: d15N
Units: permille
Description: <p>nitrogen isotope value</p>
http://lod.bco-dmo.org/id/dataset-parameter/954395.rdf
Name: d13C
Units: permille
Description: <p>carbon isotope value</p>
http://lod.bco-dmo.org/id/dataset-parameter/954396.rdf
Name: Percentage_N
Units: percent
Description: <p>percent nitrogen in tissue</p>
http://lod.bco-dmo.org/id/dataset-parameter/954397.rdf
Name: Percentage_C
Units: percent
Description: <p>percent carbon in tissue</p>
http://lod.bco-dmo.org/id/dataset-parameter/954398.rdf
Name: C_N
Units: unitless
Description: <p>carbon to nitrogen ratio in tissue</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
113256
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/de050d7f-13fb-4bae-8ff9-c4a5d79c6e35/content
download
https://doi.org/10.26008/1912/bco-dmo.954333.1
download
onLine
dataset
<p>Sponge collection:&nbsp; Sponge species were collected from at least one site within four geographic regions spanning more than 15° of latitude (~1700 km) within the Caribbean Sea. Individual regions included the Bocas del Toro archipelago of Panama, the Miskito Cays of Honduras, the Mesoamerican barrier reef of Belize, and the Florida Keys. At each site, replicate small (3–5 ml) samples of dominant and conspicuous sponge species were collected by SCUBA using a dive knife and placed into individual bags containing seawater for transport back to the laboratory. Sponge samples always included a cross section with both inner and outer tissue regions to standardize collections and sample across the entire body of the sponge. Collections frequently included eight of the ten most dominant Caribbean species&nbsp;and species previously designated as both HMA and LMA sponges. Samples were preserved, processed, and prepared for&nbsp;<em>δ</em>13C and&nbsp;<em>δ</em>15N analysis. Sponges were identified to species and, if necessary, identities were verified via tissue histology and spicule preparations. Replicate subsamples of each sponge species were also preserved in 95% EtOH in 5 ml cryovials and frozen at −20 °C for analyses of microbial community structure.</p>
<p>Stable isotope and chlorophyll&nbsp;<em>a</em>&nbsp;analyses: Stable isotope values (<em>δ</em>13C and&nbsp;<em>δ</em>15N) of bulk sponge tissue serve as a time-integrated record of the sources of carbon and nitrogen assimilated by a holobiont (including activities of both sponge and microbial cells) and any fractionation associated with symbiont or host metabolism or nutrient recycling. Within an individual reef,&nbsp;<em>δ</em>13C and&nbsp;<em>δ</em>15N values of sponge tissue therefore act as a metabolic “fingerprint” that integrates the physiological, metabolic, and ecological differences present across individual sponges.&nbsp;Bulk sponge tissue samples were analyzed in the Stable Isotope Ratio Mass Spectrometry Laboratory at the University of Hong Kong as in. Mean (±SE) precision during analysis was 0.1 (0.001) ‰ and 0.2 (0.03) ‰ for&nbsp;<em>δ</em>13C and&nbsp;<em>δ</em>15N, respectively. Isotope values are expressed in delta (<em>δ</em>) notation in units per mille (‰). Values of the elemental composition (%C, %N, and C:N) of each sample of sponge tissue were also provided. Elemental values provide important information about how biomass-associated pools of carbon and nitrogen vary across sponge species and allowed us to test whether our trends in&nbsp;<em>δ</em>13C and&nbsp;<em>δ</em>15N values were strongly influenced by structural differences in sponge tissue. Photosymbiont abundance (as determined by chlorophyll&nbsp;<em>a</em>&nbsp;[chl&nbsp;<em>a</em>] concentration) was quantified in sponges from sites in Honduras, Panama, and the Florida Keys as in&nbsp;and expressed as μg chl&nbsp;<em>a</em>&nbsp;[g dry sponge tissue]−1.&nbsp;<em>Scopalina ruetzleri</em>&nbsp;samples were not analyzed for chl&nbsp;<em>a</em>&nbsp;because they were too small to provide tissue for both isotope and chl&nbsp;<em>a</em>&nbsp;analyses.</p>
Specified by the Principal Investigator(s)
* converted date to ISO format
* Adjusted field names to comply with database requirements
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Eurovector EA3028 coupled to a Perspective IRMS (Nu Instruments)
Eurovector EA3028 coupled to a Perspective IRMS (Nu Instruments)
PI Supplied Instrument Name: Eurovector EA3028 coupled to a Perspective IRMS (Nu Instruments) PI Supplied Instrument Description:Sponge isotope samples were analyzed in the Stable Isotope Ratio Mass Spectrometry laboratory (SIRMS) at the University of Hong Kong via combustion in a Eurovector EA3028 coupled to a Perspective IRMS (Nu Instruments). Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/