http://lod.bco-dmo.org/id/dataset/954439
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-02-26
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
NanoSIMS data from sponges collected in Summerland Key in Florida between July 27 - August 19, 2021
2025-04-23
publication
2025-04-23
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-05-07
publication
https://doi.org/10.26008/1912/bco-dmo.954439.1
Cole G. Easson
Middle Tennessee State University
principalInvestigator
Cara L. Fiore
Appalachian State University
principalInvestigator
Christopher J. Freeman
College of Charleston
principalInvestigator
Robert W. Thacker
Stony Brook University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Freeman, C. J., Easson, C. G., Fiore, C. L., Thacker, R. W., Jones, H. (2025) NanoSIMS data from sponges collected in Summerland Key in Florida between July 27 - August 19, 2021. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-04-23 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.954439.1 [access date]
Caribbean sponges - nanoSIMS Dataset Description: Methods and Sampling: <p>Sample collection<strong><em>:</em></strong> Samples were collected on August 1, 2021. Lab work and experiments were conducted at Mote Marine Laboratory in Summerland Key,&nbsp;FL, USA, between July 27 and August 19, 2021. Replicate individuals of the four sponge species&nbsp;<em>Aplysina cauliformis</em>,&nbsp;<em>Iotrochota birotulata</em>,&nbsp;<em>Niphates digitalis</em>, and&nbsp;<em>Xestospongia muta</em>&nbsp;were collected from a shallow (7 m depth) patch reef (24°33’ N, 81°24’ W) offshore of Summerland Key (24°39’ N, 81°26’ W) via dive knives (with the spongocoel intact for the barrel/tube sponges by cutting the sponge from the substrate) using SCUBA, and were placed into plastic bags filled with seawater before being brought to the surface. After collection, all sponges were kept submerged in ambient seawater in large, sealed plastic bags in an insulated cooler during transit back to the lab. Upon return to Mote Marine Laboratory, sponges were attached with cable ties to plastic window screens, and held in large, flow-through raceways for 24 h for acclimatization. At no point during this process were sponges exposed to air.&nbsp;</p>
<p>Only healthy sponges (sponges confirmed to be actively pumping via use of fluorescein dye, and without any signs of necrosis) were processed for and used in experiments. For each experiment (and each treatment in the autotrophy experiment), four large individuals of each of the rope sponges&nbsp;<em>I. birotulata</em>&nbsp;and&nbsp;<em>A. cauliformis</em>&nbsp;collected from the reef were partitioned into seven subsamples for sampling at discrete time points. Both&nbsp;<em>X. muta</em>&nbsp;and&nbsp;<em>N. digitalis</em>&nbsp;were only sampled at four timepoints during each experiment because they have barrel/tube morphologies that cannot be successfully partitioned and subsampled, requiring the sacrifice of an entire individual for each timepoint. Therefore, sixteen small individuals of each of these two species were collected from the reef for each experiment. Experimental replicates were defined as follows: a replicate of&nbsp;<em>X. muta</em>&nbsp;consisted of one individual attached to a plastic window screen, which was placed in an 8 L, clear food-grade container; a replicate of&nbsp;<em>N. digitalis</em>&nbsp;consisted of four small individuals attached to a plastic window screen, which was placed into a 17.9 L, clear food-grade container; and replicates of&nbsp;<em>A. cauliformis</em>&nbsp;and&nbsp;<em>I. birotulata</em>&nbsp;consisted of all seven subsamples from the same individual attached to a plastic window screen, which was placed into a 17.9 L, clear food-grade container. In total, there were four replicates of each species collected at each time point in each experiment, with the exception the DOM experiment, during which multiple individuals, including all individuals of&nbsp;<em>A. cauliformis</em>,<em>&nbsp;</em>developed necroses in experimental tanks after 24h. The effected individuals were eliminated, limiting replication to only two timepoints (24h and 72h) during the chase for all species. Due to necroses of all individuals of&nbsp;<em>A. cauliformis&nbsp;</em>after 24h, the DOM experiment was repeated for this species only, using three healthy replicates.</p>
<p>Pulse-chase experiments<strong><em>:</em></strong>&nbsp;Three separate “pulse-chase” experiments were carried out to track the uptake and recycling of labeled compounds from three different resource pools. The first resource pool, DOM, consisted of&nbsp;<sup>13</sup>C-labeled glucose (35 μM) and galactose (35 μM), and&nbsp;<sup>13</sup>C and&nbsp;<sup>15</sup>N-labeled cell-free amino acids (range from 39-790 nM) and urea (2.2 μM). The second resource pool, POM, consisted of&nbsp;<sup>15</sup>N- and&nbsp;<sup>13</sup>C-labeled&nbsp;<em>Synechococcus&nbsp;</em>spp. bacteria incubated for 36 h in seawater containing 1.18 μM NaH<sup>13</sup>CO₃, 3.67 μM&nbsp;<sup>15</sup>NH₄Cl, and 0.117 M Na<sup>15</sup>NO₃, and&nbsp;<em>Oceanicola batsensis&nbsp;</em>incubated in 0.55 μM galactose, 1.18 μM NaH<sup>13</sup>CO₃, 3.67 μM&nbsp;<sup>15</sup>NH₄Cl, and 0.117 μM Na<sup>15</sup>NO₃. Finally, the third resource pool of inorganic compounds consisted of Na<sup>15</sup>NO₃ (0.117 μM) and NaH<sup>13</sup>CO₃ (1.18 μM). Labeled bacterial cells were added to filtered seawater for the POM experiment at a final combined concentration of 4.65 x10<sup>5</sup>&nbsp;cells ml<sup>-1</sup>&nbsp;for photosynthetic cells and 1.01 x 10<sup>6</sup>&nbsp;for heterotrophic cells (measured at the Center for Aquatic Cytometry at Bigelow Laboratory for Ocean Sciences). Concentrations of labeled resources were chosen to approximate the concentrations of corresponding inorganic and organic sources of carbon and nitrogen found in Caribbean seawater as closely as possible, as well as to match concentrations used in previous studies. In all incubations, experimental water was 0.7 μm (GF/F) filtered prior to the addition of tracers or labeled bacteria. Sponges were “pulsed” for 3 h in water containing these isotopically enriched resources, and then sponges were held in unenriched, flowing seawater for a 72-h “chase” period. Experimental samples were collected during the pulse at t=0.5 h, and during the chase at final times of t=12 h, 48 h, and 75 h (minus the 48h sampling in the DOM experiment). Prior to the start of the pulse, one of the seven subsamples of&nbsp;<em>A. cauliformis</em>&nbsp;and&nbsp;<em>I. birotulata</em>, and four individuals each of&nbsp;<em>X. muta</em>&nbsp;and&nbsp;<em>N. digitalis</em>&nbsp;were sampled and processed to serve as t = 0 h (natural abundance) samples.</p>
<p>To start the pulse, each experimental replicate was added to a food grade container containing filtered seawater and labeled compounds or food bacteria, as well as a recirculating pump used to maintain flow. Replicates of&nbsp;<em>X. muta</em>were held in 8 L, clear food grade containers holding 6 L of filtered seawater, while replicates of&nbsp;<em>I. birotulata</em>,&nbsp;<em>A. cauliformis</em>, and&nbsp;<em>N. digitalis</em>&nbsp;were held in 17.9 L containers holding 15 L of filtered seawater. These containers were all held in a large 4.6 m-diameter outdoor tank with a shallow seawater bath to control temperature in experimental tanks holding sponges. At the end of the pulse, the water level in the outdoor tank was raised to flood experimental tanks with unlabeled seawater (supplied from the canal adjacent to Mote Marine Lab, drawn from 10 m, and filtered through sand to 40 μm) throughout the chase. Temperature measurements were taken at regular intervals (30 min), and an additional 25°C supply of well water, with salinity equal to that of seawater, was supplemented to maintain a tank temperature between 29-32°C. During the day for all experiments, irradiance measurements were taken every 30 min to monitor for deviation outside of the irradiance that sponges experience at depth, which was 800 to 1000 μmol photons m<sup>-2</sup>&nbsp;s<sup>-1</sup>&nbsp;measured at depth during cloudless daytime conditions at the collection site. One layer of standard shade cloth was sufficient to keep irradiance within the normal limits of what the sponges experience at depth, and adjustments during the experiment were only made when irradiance went below the 800-1000 μmol photons m<sup>-2</sup>&nbsp;s<sup>-1</sup>&nbsp;range under cloudy conditions. When irradiance decreased, we removed the layer of shade cloth to increase exposure of the sponges to ambient sunlight.</p>
<p>To assess the role of both autotrophic and heterotrophic symbionts in the assimilation of inorganic compounds, the autotrophy experiment included both light and dark treatments. For this reason, each large individual of&nbsp;<em>I. birotulata</em>&nbsp;and&nbsp;<em>A. cauliformis&nbsp;</em>was partitioned into 14 subsamples, and 32 small individuals each of&nbsp;<em>N. digitalis&nbsp;</em>and&nbsp;<em>X. muta</em>&nbsp;were collected for this experiment. Half of the subsamples or individuals of each species were randomly assigned to a dark treatment (covered in opaque plastic tarp with irradiance values from HOBO loggers that detected 0 Lux throughout the pulse), and the other half to a light treatment (exposure to ambient irradiance as in other experiments). Samples in the dark treatment were covered during the pulse but were exposed to ambient irradiance during the chase so that we could monitor nutrient retention and recycling under ambient (light-dark cycle) conditions.</p>
<p>Sample fixation<strong><em>:</em></strong>&nbsp;After sampling during pulse-chase experiments, sponges were rinsed with 0.7 μm-filtered, unenriched seawater and lightly blotted with a paper towel before weighing to the nearest 0.001 g to obtain a wet weight. Afterwards, ~2 mm cross sections were collected in duplicate using a razor blade and placed into 2 mL cryovials to be fixed for SEM and NanoSIMS analysis; the remaining bulk tissue was frozen at -20°C for isotope analysis. The 2 mm cross-sections of sponge tissue were fixed in&nbsp;2.5% (w/v) glutaraldehyde + 1% (w/v) paraformaldehyde in 1.5X&nbsp;PHEM buffer (1.5X PHEM (90 mM PIPES, 37.5 mM HEPES, 15 mM EGTA, 3 mM&nbsp;MgSO4.7H<sub>2</sub>O), and 9% (w/v) sucrose at pH 7.4) and stored at 4°C&nbsp;for 12h before being rinsed 3x with PHEM buffer containing no fixative. Finally, 0.1 mL of fixative solution (10% of the concentration in the original solution) was added back to each sample to prevent any fouling during long-term storage at&nbsp;4°C.&nbsp;After being taken out of storage,&nbsp;cross-sections of sponge tissue were rinsed once more in fixative-free 1.5X PHEM buffer, digested in 5% hydrofluoric acid to break down siliceous spicules within the sponge tissue (note this was carried out for all samples to be consistent, even though&nbsp;<em>A. cauliformis</em>&nbsp;lacks spicules), and rinsed twice and stored in 1.5X PHEM buffer.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1929293 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1929293
completed
Cole G. Easson
Middle Tennessee State University
615-898-5822
1672 Greenland Drive SCI 2044
Murfreesboro
TN
37132
USA
Cole.Easson@mtsu.edu
pointOfContact
Cara L. Fiore
Appalachian State University
828-262-7548
572 Rivers St
Boone
NC
28607
USA
fiorec@appstate.edu
pointOfContact
Christopher J. Freeman
College of Charleston
817-707-5840
66 George Street Department of Biology
Charleston
SC
29424
USA
freemancj@cofc.edu
pointOfContact
Robert W. Thacker
Stony Brook University
631-632-9949
West 5510 FRK MEL LIB
Stony Brook
NY
11794
USA
robert.thacker@stonybrook.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Roi_name
Roi_group
area
mean13C
stddev13C
R_13C12C_12C2
corr_R_13C_13C
d_13C_12C
species
Collection_date
time
SEM_region
mean15N
stddev15N
R_15N_14N
corr_R_15N_14N
d_15N_14N
experiment
lat
lon
theme
None, User defined
sample identification
sample description
area
d13C
species scientific name (binomial)
date
sample time
d15N
experiment type
latitude
longitude
featureType
BCO-DMO Standard Parameters
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Investigations into microbially mediated ecological diversification in sponges
https://osprey.bco-dmo.org/project/772220
Collaborative Research: Investigations into microbially mediated ecological diversification in sponges
<p><em>NSF Award Abstract:</em><br />
Coral reefs represent a paradox because, despite their immense productivity and biodiversity, they are found in nutrient-poor habitats that are equivalent to "marine deserts." High biodiversity is often associated with a division of resources that allows many types of organisms to coexist with minimal competition. Indeed, unlike many other organisms on coral reefs, sponges are adapted to efficiently remove bacteria, phytoplankton, and dissolved organic matter from seawater by filter-feeding. Sponges are a dominant component of coral reefs worldwide and in the Caribbean, where their biomass exceeds that of reef-building corals. For almost a quarter century, the success of sponges in the Caribbean has been linked to their filter-feeding ability. However, recent work demonstrated that coexisting sponges on Caribbean reefs host unique communities of bacteria that might allow sponges to access multiple pools of nutrients that are not available to other organisms. In this project, the investigators will test the hypothesis that ecologically dominant sponge species in the Caribbean have unique metabolic strategies that are mediated by their associations with microbes that live within the sponge body. This research will combine manipulative field experiments with a novel combination of modern analytical tools to investigate both filter-feeding by sponge hosts and the metabolic pathways of their microbes. This work will advance our understanding of the ecological and evolutionary forces that have helped shape the species present on Caribbean coral reefs. Additionally, this project will support three early-career investigators and provide training opportunities for graduate and undergraduate students at Nova Southeastern University, Appalachian State University, Stony Brook University, and Smithsonian Marine Station. The investigators will also develop innovative outreach programs that expand existing platforms at their institutions to increase public engagement and scientific literacy.</p>
<p>Marine sponges have been widely successful in their expansion across ecological niches in the Caribbean, with biomass often exceeding that of reef-building corals and high species diversity. However, whether this success is linked to efficient heterotrophic filter-feeding on organic carbon in the water column or to their evolutionary investment in microbial symbionts is yet to be fully elucidated. Microbial symbionts expand the metabolic capabilities of host sponges, supplementing heterotrophic feeding with inorganic carbon and nitrogen, mediating the assimilation of dissolved organic matter, and facilitating recycling of host-derived nitrogen. Despite these benefits, microbial symbiont communities are widely divergent across coexisting sponge species and there is substantial variation in host reliance on symbiont-derived carbon and nitrogen among host sponges; therefore, these associations likely mediate the ecological diversification of coexisting sponge species. The goal of this project is to test this transformative hypothesis by adopting an integrative approach to assess the individual components of holobiont metabolism (i.e., microbial symbionts and sponge host) in ten of the most common sponge species in the Caribbean. The investigators will isolate autotrophic and heterotrophic metabolic pathways and explore potential links between microbial symbiont community composition and the assimilation of particulate and dissolved organic matter (POM and DOM) from seawater. This project will elucidate whether Caribbean sponge species are on similar or divergent evolutionary trajectories, and will provide information that is critical for our understanding of how conditions in the Caribbean basin have shaped the evolution of benthic organisms.</p>
Ecological Diversification in Sponges
largerWorkCitation
project
eng; USA
oceans
-81.40839451
-81.40839451
24.56381897
24.56381897
2021-07-27
2021-08-19
Caribbean coast of Panama
0
BCO-DMO catalogue of parameters from NanoSIMS data from sponges collected in Summerland Key in Florida between July 27 - August 19, 2021
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/954450.rdf
Name: Roi_name
Units: unitless
Description: <p>number of roi (region of interest) within a given sample - each roi corresponds to one cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/954451.rdf
Name: Roi_group
Units: unitless
Description: <p>gives cell type (various host cell types (choanocytes, pinacocytes, c chamber [choanocyte chamber] vs symbiont cells) of each roi</p>
http://lod.bco-dmo.org/id/dataset-parameter/954452.rdf
Name: area
Units: pixels^2
Description: <p>gives number of pixels within each roi from a 256x256 pixel image</p>
http://lod.bco-dmo.org/id/dataset-parameter/954453.rdf
Name: mean13C
Units: ratio (x10000)
Description: <p>mean 13C12C/12C2 ratio (x10000) in each roi</p>
http://lod.bco-dmo.org/id/dataset-parameter/954454.rdf
Name: stddev13C
Units: ratio (x10000)
Description: <p>standard deviation of 13C12C/12C2 ratio (x10000) in each roi</p>
http://lod.bco-dmo.org/id/dataset-parameter/954455.rdf
Name: R_13C12C_12C2
Units: ratio
Description: <p>mean 13C12C/12C2 ratio in each roi</p>
http://lod.bco-dmo.org/id/dataset-parameter/954456.rdf
Name: corr_R_13C_13C
Units: ratio
Description: <p>same as "R 13C12C/12C2" but corrected for machine drift and divided by 2 to give 13C:12C ratio in each roi</p>
http://lod.bco-dmo.org/id/dataset-parameter/954457.rdf
Name: d_13C_12C
Units: delta 13C in permille
Description: <p>delta 13C value of each roi calculated using an unlabeled bulk tissue sample as a control</p>
http://lod.bco-dmo.org/id/dataset-parameter/954458.rdf
Name: species
Units: unitless
Description: <p>sponge species from which tissue sampe was taken; Aplysina cauliformis or Iotrochota birotulata</p>
http://lod.bco-dmo.org/id/dataset-parameter/954459.rdf
Name: Collection_date
Units: unitless
Description: <p>Collection date of sponge samples</p>
http://lod.bco-dmo.org/id/dataset-parameter/954460.rdf
Name: time
Units: unitless
Description: <p>timepoint that sample was collected during experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/954461.rdf
Name: SEM_region
Units: unitless
Description: <p>numbered region within tissue crossection of the correlated Scanning Electron Microscopy image for each NanoSIMS image.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954462.rdf
Name: mean15N
Units: ratio (x10000)
Description: <p>mean 15N12C/14N12C ratio (x10000) in each roi</p>
http://lod.bco-dmo.org/id/dataset-parameter/954463.rdf
Name: stddev15N
Units: ratio (x10000)
Description: <p>standard deviation of 15N12C/14N12C ratio (x10000) in each roi</p>
http://lod.bco-dmo.org/id/dataset-parameter/954464.rdf
Name: R_15N_14N
Units: ratio
Description: <p>mean 15N12C/14N12C ratio in each roi</p>
http://lod.bco-dmo.org/id/dataset-parameter/954465.rdf
Name: corr_R_15N_14N
Units: ratio
Description: <p>same as "R 15N/14N" but corrected for machine drift</p>
http://lod.bco-dmo.org/id/dataset-parameter/954466.rdf
Name: d_15N_14N
Units: delta 15N in permille
Description: <p>delta 15N value of each roi calculated using an unlabeled bulk tissue sample as a control</p>
http://lod.bco-dmo.org/id/dataset-parameter/954467.rdf
Name: experiment
Units: unitless
Description: <p>AD, AL, DOM, and POM signify if samples were collected during experiments focused on uptake of: inorganic nutrients in darkness, inorganic nutrients in the light, dissolved organic matter, and particulate organic matter, respectively.</p>
http://lod.bco-dmo.org/id/dataset-parameter/954468.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude of collection site for sponge individuals, south is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/954469.rdf
Name: lon
Units: decimal degrees
Description: <p>latitude of collection site for sponge individuals, south is negative</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1971596
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/6a20775c-5d85-40d6-940f-61cb961ad9ae/content
download
https://doi.org/10.26008/1912/bco-dmo.954439.1
download
onLine
dataset
<p>Sample collection<strong><em>:</em></strong> Samples were collected on August 1, 2021. Lab work and experiments were conducted at Mote Marine Laboratory in Summerland Key,&nbsp;FL, USA, between July 27 and August 19, 2021. Replicate individuals of the four sponge species&nbsp;<em>Aplysina cauliformis</em>,&nbsp;<em>Iotrochota birotulata</em>,&nbsp;<em>Niphates digitalis</em>, and&nbsp;<em>Xestospongia muta</em>&nbsp;were collected from a shallow (7 m depth) patch reef (24°33’ N, 81°24’ W) offshore of Summerland Key (24°39’ N, 81°26’ W) via dive knives (with the spongocoel intact for the barrel/tube sponges by cutting the sponge from the substrate) using SCUBA, and were placed into plastic bags filled with seawater before being brought to the surface. After collection, all sponges were kept submerged in ambient seawater in large, sealed plastic bags in an insulated cooler during transit back to the lab. Upon return to Mote Marine Laboratory, sponges were attached with cable ties to plastic window screens, and held in large, flow-through raceways for 24 h for acclimatization. At no point during this process were sponges exposed to air.&nbsp;</p>
<p>Only healthy sponges (sponges confirmed to be actively pumping via use of fluorescein dye, and without any signs of necrosis) were processed for and used in experiments. For each experiment (and each treatment in the autotrophy experiment), four large individuals of each of the rope sponges&nbsp;<em>I. birotulata</em>&nbsp;and&nbsp;<em>A. cauliformis</em>&nbsp;collected from the reef were partitioned into seven subsamples for sampling at discrete time points. Both&nbsp;<em>X. muta</em>&nbsp;and&nbsp;<em>N. digitalis</em>&nbsp;were only sampled at four timepoints during each experiment because they have barrel/tube morphologies that cannot be successfully partitioned and subsampled, requiring the sacrifice of an entire individual for each timepoint. Therefore, sixteen small individuals of each of these two species were collected from the reef for each experiment. Experimental replicates were defined as follows: a replicate of&nbsp;<em>X. muta</em>&nbsp;consisted of one individual attached to a plastic window screen, which was placed in an 8 L, clear food-grade container; a replicate of&nbsp;<em>N. digitalis</em>&nbsp;consisted of four small individuals attached to a plastic window screen, which was placed into a 17.9 L, clear food-grade container; and replicates of&nbsp;<em>A. cauliformis</em>&nbsp;and&nbsp;<em>I. birotulata</em>&nbsp;consisted of all seven subsamples from the same individual attached to a plastic window screen, which was placed into a 17.9 L, clear food-grade container. In total, there were four replicates of each species collected at each time point in each experiment, with the exception the DOM experiment, during which multiple individuals, including all individuals of&nbsp;<em>A. cauliformis</em>,<em>&nbsp;</em>developed necroses in experimental tanks after 24h. The effected individuals were eliminated, limiting replication to only two timepoints (24h and 72h) during the chase for all species. Due to necroses of all individuals of&nbsp;<em>A. cauliformis&nbsp;</em>after 24h, the DOM experiment was repeated for this species only, using three healthy replicates.</p>
<p>Pulse-chase experiments<strong><em>:</em></strong>&nbsp;Three separate “pulse-chase” experiments were carried out to track the uptake and recycling of labeled compounds from three different resource pools. The first resource pool, DOM, consisted of&nbsp;<sup>13</sup>C-labeled glucose (35 μM) and galactose (35 μM), and&nbsp;<sup>13</sup>C and&nbsp;<sup>15</sup>N-labeled cell-free amino acids (range from 39-790 nM) and urea (2.2 μM). The second resource pool, POM, consisted of&nbsp;<sup>15</sup>N- and&nbsp;<sup>13</sup>C-labeled&nbsp;<em>Synechococcus&nbsp;</em>spp. bacteria incubated for 36 h in seawater containing 1.18 μM NaH<sup>13</sup>CO₃, 3.67 μM&nbsp;<sup>15</sup>NH₄Cl, and 0.117 M Na<sup>15</sup>NO₃, and&nbsp;<em>Oceanicola batsensis&nbsp;</em>incubated in 0.55 μM galactose, 1.18 μM NaH<sup>13</sup>CO₃, 3.67 μM&nbsp;<sup>15</sup>NH₄Cl, and 0.117 μM Na<sup>15</sup>NO₃. Finally, the third resource pool of inorganic compounds consisted of Na<sup>15</sup>NO₃ (0.117 μM) and NaH<sup>13</sup>CO₃ (1.18 μM). Labeled bacterial cells were added to filtered seawater for the POM experiment at a final combined concentration of 4.65 x10<sup>5</sup>&nbsp;cells ml<sup>-1</sup>&nbsp;for photosynthetic cells and 1.01 x 10<sup>6</sup>&nbsp;for heterotrophic cells (measured at the Center for Aquatic Cytometry at Bigelow Laboratory for Ocean Sciences). Concentrations of labeled resources were chosen to approximate the concentrations of corresponding inorganic and organic sources of carbon and nitrogen found in Caribbean seawater as closely as possible, as well as to match concentrations used in previous studies. In all incubations, experimental water was 0.7 μm (GF/F) filtered prior to the addition of tracers or labeled bacteria. Sponges were “pulsed” for 3 h in water containing these isotopically enriched resources, and then sponges were held in unenriched, flowing seawater for a 72-h “chase” period. Experimental samples were collected during the pulse at t=0.5 h, and during the chase at final times of t=12 h, 48 h, and 75 h (minus the 48h sampling in the DOM experiment). Prior to the start of the pulse, one of the seven subsamples of&nbsp;<em>A. cauliformis</em>&nbsp;and&nbsp;<em>I. birotulata</em>, and four individuals each of&nbsp;<em>X. muta</em>&nbsp;and&nbsp;<em>N. digitalis</em>&nbsp;were sampled and processed to serve as t = 0 h (natural abundance) samples.</p>
<p>To start the pulse, each experimental replicate was added to a food grade container containing filtered seawater and labeled compounds or food bacteria, as well as a recirculating pump used to maintain flow. Replicates of&nbsp;<em>X. muta</em>were held in 8 L, clear food grade containers holding 6 L of filtered seawater, while replicates of&nbsp;<em>I. birotulata</em>,&nbsp;<em>A. cauliformis</em>, and&nbsp;<em>N. digitalis</em>&nbsp;were held in 17.9 L containers holding 15 L of filtered seawater. These containers were all held in a large 4.6 m-diameter outdoor tank with a shallow seawater bath to control temperature in experimental tanks holding sponges. At the end of the pulse, the water level in the outdoor tank was raised to flood experimental tanks with unlabeled seawater (supplied from the canal adjacent to Mote Marine Lab, drawn from 10 m, and filtered through sand to 40 μm) throughout the chase. Temperature measurements were taken at regular intervals (30 min), and an additional 25°C supply of well water, with salinity equal to that of seawater, was supplemented to maintain a tank temperature between 29-32°C. During the day for all experiments, irradiance measurements were taken every 30 min to monitor for deviation outside of the irradiance that sponges experience at depth, which was 800 to 1000 μmol photons m<sup>-2</sup>&nbsp;s<sup>-1</sup>&nbsp;measured at depth during cloudless daytime conditions at the collection site. One layer of standard shade cloth was sufficient to keep irradiance within the normal limits of what the sponges experience at depth, and adjustments during the experiment were only made when irradiance went below the 800-1000 μmol photons m<sup>-2</sup>&nbsp;s<sup>-1</sup>&nbsp;range under cloudy conditions. When irradiance decreased, we removed the layer of shade cloth to increase exposure of the sponges to ambient sunlight.</p>
<p>To assess the role of both autotrophic and heterotrophic symbionts in the assimilation of inorganic compounds, the autotrophy experiment included both light and dark treatments. For this reason, each large individual of&nbsp;<em>I. birotulata</em>&nbsp;and&nbsp;<em>A. cauliformis&nbsp;</em>was partitioned into 14 subsamples, and 32 small individuals each of&nbsp;<em>N. digitalis&nbsp;</em>and&nbsp;<em>X. muta</em>&nbsp;were collected for this experiment. Half of the subsamples or individuals of each species were randomly assigned to a dark treatment (covered in opaque plastic tarp with irradiance values from HOBO loggers that detected 0 Lux throughout the pulse), and the other half to a light treatment (exposure to ambient irradiance as in other experiments). Samples in the dark treatment were covered during the pulse but were exposed to ambient irradiance during the chase so that we could monitor nutrient retention and recycling under ambient (light-dark cycle) conditions.</p>
<p>Sample fixation<strong><em>:</em></strong>&nbsp;After sampling during pulse-chase experiments, sponges were rinsed with 0.7 μm-filtered, unenriched seawater and lightly blotted with a paper towel before weighing to the nearest 0.001 g to obtain a wet weight. Afterwards, ~2 mm cross sections were collected in duplicate using a razor blade and placed into 2 mL cryovials to be fixed for SEM and NanoSIMS analysis; the remaining bulk tissue was frozen at -20°C for isotope analysis. The 2 mm cross-sections of sponge tissue were fixed in&nbsp;2.5% (w/v) glutaraldehyde + 1% (w/v) paraformaldehyde in 1.5X&nbsp;PHEM buffer (1.5X PHEM (90 mM PIPES, 37.5 mM HEPES, 15 mM EGTA, 3 mM&nbsp;MgSO4.7H<sub>2</sub>O), and 9% (w/v) sucrose at pH 7.4) and stored at 4°C&nbsp;for 12h before being rinsed 3x with PHEM buffer containing no fixative. Finally, 0.1 mL of fixative solution (10% of the concentration in the original solution) was added back to each sample to prevent any fouling during long-term storage at&nbsp;4°C.&nbsp;After being taken out of storage,&nbsp;cross-sections of sponge tissue were rinsed once more in fixative-free 1.5X PHEM buffer, digested in 5% hydrofluoric acid to break down siliceous spicules within the sponge tissue (note this was carried out for all samples to be consistent, even though&nbsp;<em>A. cauliformis</em>&nbsp;lacks spicules), and rinsed twice and stored in 1.5X PHEM buffer.</p>
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<p>Scanning electron microscopy and nanoSIMS<strong><em>:</em></strong>&nbsp;Due to the high cost of SEM and especially NanoSIMS analysis, only one HMA species (<em>A. cauliformis</em>) and one LMA species (<em>I. birotulata</em>) from each experiment was selected for high-resolution analyses, and only four time-points (including t=0h) were included.&nbsp;One replicate each of&nbsp;<em>A. cauliformis</em>&nbsp;and&nbsp;<em>I. birotulata</em>&nbsp;per time point per experiment was analyzed via nanoSIMS; these species were chosen based on better histological preservation than&nbsp;<em>X. muta&nbsp;</em>and&nbsp;<em>N. digitalis</em>&nbsp;as evaluated during electron microscopy, and replicates were selected based on median enrichment of bulk tissue to approximate the mean as closely as possible. Samples from t = 0 h, 0.5 h, 12 h, and 75 h were analyzed from each experiment, with at least three different areas containing diverse cell types analyzed per sample. Individual cells identified for analysis are referred to as regions of interest (ROI), and a total of 9,179 ROI were analyzed across 26 holobiont samples, including natural abundance samples.</p>
<p>To prepare samples for SEM and NanoSIMS, similar methods to Hudspith et al.<sup>10</sup>&nbsp;were followed. Cross-sections stored in 1.5X PHEM buffer were post-fixed for 1.5 h with 1 % (w/v) osmium tetroxide before being dehydrated with a graded ethanol series and embedded in Embed 812.&nbsp;Semithin (500 nm) sections were cut using an RMC Products Powertome XL ultramicrotome at the Electron Microscopy Center at Clemson University, Anderson, SC, USA. Semithin sections were then mounted onto silicon wafers and stained with Uranyless (a non-radioactive substitute for uranyl acetate) and lead citrate for SEM analysis. Scanning electron microscopy (SEM) was carried out to find areas within sponge tissue containing diverse cell types, provide sample maps, and serve as a correlative method to confidently identify individual ROI analyzed during NanoSIMS. SEM was performed at Clemson University’s Warren Lasch Conservation Center in North Charleston, SC, USA, using a Hitachi S-3700N SEM set to backscattered electron (BSE) imaging at an accelerating voltage of 5 kV. SEM images were taken at a maximum magnification of 3,000X, with several images of the same area taken at lower magnification so that finding areas of interest during NanoSIMS was possible. All SEM images included at least one choanocyte chamber with multiple mesohyl cells and symbiont cells within 30&nbsp;μm of the choanocyte chamber, allowing for efficient analysis of diverse cell types during NanoSIMS.</p>
<p>After SEM was complete, samples were coated with 5 nm gold-palladium for NanoSIMS analysis at the Nano Shared Facilities Laboratory at Stanford University, Stanford, CA, USA. NanoSIMS analysis was performed using a CAMECA 50L nanoscale secondary ion mass spectrometer to visualize cellular enrichment of individual host and symbiont cells. During NanoSIMS analysis, samples were bombarded with a 16kV Cs<sup>+</sup>&nbsp;primary ion beam to detect the secondary ions of&nbsp;<sup>12</sup>C<sup>12</sup>C<sup>-</sup>,&nbsp;<sup>12</sup>C<sup>13</sup>C<sup>-</sup>,&nbsp;<sup>12</sup>C<sup>14</sup>N<sup>-</sup>,&nbsp;<sup>12</sup>C<sup>15</sup>N<sup>-</sup>, using a mass resolving power of at least 7,000. Raster scans of 30 µm and 256 x 256 pixels were taken using 1 ms beam dwell time per pixel and repeated 50 times.</p>
<p>NanoSIMS images were processed using openMIMS software for Fiji. Individual ROI were manually drawn by outlining individual cells, and cells were identified as choanocytes, mesohyl cells, symbiont cells, or cyanobacteria. Mesohyl cells here include multiple cell types, such as amoebocytes and sclerocytes, though spherulous cells were excluded as minimal enrichment was observed within any of these cells, which is consistent with previous studies. SEM images were used to confirm cell identifications. Daily scans of an unenriched control sample of sponge tissue were taken. Enrichment values of each daily control scan were compared to the bulk enrichment measured from the same sample to calculate a correction factor:</p>
<p>to compensate for any machine drift during NanoSIMS analysis, where R<sub>sample</sub>IRMS represents the&nbsp;<sup>12</sup>C<sup>15</sup>N/<sup>12</sup>C<sup>14</sup>N or&nbsp;<sup>12</sup>C<sup>13</sup>C/<sup>12</sup>C<sup>12</sup>C ratio measured during bulk tissue analysis, and R<sub>sample</sub>nanoSIMS represents the&nbsp;<sup>12</sup>C<sup>15</sup>N/<sup>12</sup>C<sup>14</sup>N or&nbsp;<sup>12</sup>C<sup>13</sup>C/<sup>12</sup>C<sup>12</sup>C ratio measured during each daily scan of the same unenriched control sample. There were four days (out of twenty-one days during which NanoSIMS analyses were conducted) that control scans were not taken, so the mean correction factor (mean ± SD CF for&nbsp;<sup>12</sup>C<sup>15</sup>N<sup>-</sup>/<sup>12</sup>C<sup>14</sup>N<sup>-</sup>&nbsp;was 1.00 ± 0.01, and for&nbsp;<sup>12</sup>C<sup>13</sup>C<sup>-</sup>/<sup>12</sup>C<sup>12</sup>C<sup>-</sup>&nbsp;was 1.05 ± 0.02) was applied to these samples.</p>
<p>To determine whether a cell was considered enriched, the isotope value of each cell analyzed within experimental samples was&nbsp;compared to the mean isotope value of all cells of the same type within the same sponge species from unenriched control (t=0h) samples, using a conservative threshold of three standard deviations above mean natural abundance values as the differentiator between enriched vs unenriched cells. Due to the high cost and low throughput of NanoSIMS analysis, replication at the holobiont level is often quite limited. Thus, while multiple areas were imaged within each sample, only one replicate per species per timepoint per experiment was analyzed, so no statistical analyses are presented here. Instead, descriptive statistics, including mean enrichment of the different ROI cell groups associated with each species at each time point, as well as the percentage of cells within each group that were above the enrichment threshold are used to describe trends in the uptake and retention of&nbsp;<sup>13</sup>C and&nbsp;<sup>15</sup>N derived from different resources in each symbiosis.</p>
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