http://lod.bco-dmo.org/id/dataset/955164
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-03-01
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Magnesium isotopic compositions of seawater, extrapallial fluid, and Crassostrea virginica shell material from ocean acidification experiments
2025-03-01
publication
2025-03-01
revision
BCO-DMO Linked Data URI
2025-03-01
creation
http://lod.bco-dmo.org/id/dataset/955164
Dr Robert Eagle
University of California-Los Angeles
principalInvestigator
Katie Lotterhos
Northeastern University
principalInvestigator
Justin B. Ries
Northeastern University
principalInvestigator
Blanca Alvarez Caraveo
University of California-Los Angeles
principalInvestigator
Alan Downey-Wall
Northeastern University
principalInvestigator
Maxence Guillermic
University of California-Los Angeles
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Eagle, R., Ries, J. B., Lotterhos, K., Alvarez Caraveo, B., Guillermic, M., Downey-Wall, A. (2025) Magnesium isotopic compositions of seawater, extrapallial fluid, and Crassostrea virginica shell material from ocean acidification experiments. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-03-01 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/955164 [access date]
delta 26 Mg from OA experiment Dataset Description: <p>This dataset of magnesium isotopic compositions of seawater, extrapallial fluid, and Crassostrea virginica shell material is part of a larger ocean acidification study examining the geochemistry of carbonates with changing environmental conditions.&nbsp;</p> Methods and Sampling: <p>Ocean acidification laboratory experiments were conducted at [____place___] during [____time range___] to examine the effects of ambient (500 ppm CO<sub>2</sub>), moderate (900 ppm CO<sub>2</sub>), and high (2800 ppm CO<sub>2</sub>) ocean acidification conditions on the geochemistry of seawater, extrapallial fluid (EPF) and shells of the Eastern oyster, <em>Crassostrea virginica</em>.&nbsp;</p>
<p><em><strong>[More details are needed here on experimental design, set-up, instruments, etc.]</strong></em></p>
<p>Here we present magnesium isotope data for three biomineralization reservoirs--seawater, EPF, and Crassostrea virginica shells.&nbsp;</p>
<p><em><strong>[More details are needed]</strong></em></p>
<p>Measured on an isotope ratio mass spectrometer [<em><strong>provide make, model, and lab]</strong></em>&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1635423 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1635423
onGoing
Dr Robert Eagle
University of California-Los Angeles
310-825-3880
595 Charles Young Drive East, Box 951567 Slichter 2863
Los Angeles
CA
90095-1567
United States
rob.eagle@gmail.com
pointOfContact
Katie Lotterhos
Northeastern University
781-581-7370 X304
Northeastern University Marine Sciences Center 430 Nahant Rd.
Nahant
MA
01908
USA
k.lotterhos@northeastern.edu
pointOfContact
Justin B. Ries
Northeastern University
781-581-7370
Northeastern University Marine Science Center 430 Nahant Rd.
Nahant
MA
01908
USA
j.ries@northeastern.edu
pointOfContact
Blanca Alvarez Caraveo
University of California-Los Angeles
4244774846
alvarezblanca@g.ucla.edu
pointOfContact
Alan Downey-Wall
Northeastern University
adw@bu.edu
pointOfContact
Maxence Guillermic
University of California-Los Angeles
University of California Los Angeles, Earth, Planetary and Space Sciences 595 Charles E Young Dr E
Los Angeles
CA
90095
maxence.guillermic@gmail.com
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample _Label
pCO2
Sample_type
N
delta_26Mg
SD
????
theme
None, User defined
sample identification
Partial pressure of CO2
sample type
number
Magnesium
standard deviation
featureType
BCO-DMO Standard Parameters
Isotope-ratio Mass Spectrometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Does ocean acidification induce a methylation response that affects the fitness of the next generation in oysters?
https://osprey.bco-dmo.org/project/666496
Collaborative Research: Does ocean acidification induce a methylation response that affects the fitness of the next generation in oysters?
<p><em>NSF Award Abstract:</em><br />
Marine ecosystems worldwide are threatened by ocean acidification, a process caused by the unprecedented rate at which carbon dioxide is increasing in the atmosphere. Since ocean change is predicted to be rapid, extreme, and widespread, marine species may face an "adapt-or-die" scenario. However, modifications to the DNA sequence may be induced in response to a stress like ocean acidification and then inherited. Such "epigenetic" modifications may hold the key to population viability under global climate change, but they have been understudied. The aim of this research is to characterize the role of DNA methylation, a heritable epigenetic system, in the response of Eastern oysters (Crassostrea virginica) to ocean acidification. The intellectual merit lies in the integrative approach, which will characterize the role of DNA methylation in the intergenerational response of oysters to ocean acidification. These interdisciplinary data, spanning from molecular to organismal levels, will provide insight into mechanisms that underlie the capacity of marine invertebrates to respond to ocean acidification and lay the foundation for future transgenerational studies. Ocean acidification currently threatens marine species worldwide and has already caused significant losses in aquaculture, especially in Crassostrea species. This research has broader impacts for breeding, aquaculture, and the economy. Under the investigators' "Epigenetics to Ocean" (E2O) training program, the investigators will build STEM talent in bioinformatics and biogeochemistry, expose girls in low-income school districts to careers in genomics, and advance the field through open science and reproducibility.</p>
<p>This research will specifically test if intermittent exposure to low pH induces a methylation response with downstream beneficial effects for biomineralization. These methylation states could be inherited and confer a fitness advantage to larvae that possess them. Phase 1 of the project will use an exposure experiment to determine the degree to which DNA methylation is altered and regulates the response to OA. Data from this experiment will be used to test the hypotheses that (i) DNA methylation, induced in the tissue of shell formation (i.e., mantle tissue), is correlated with changes in transcription and regulation of pallial fluid pH (calcifying fluid pH, measured by microelectrode), and (ii) that methylation changes induced in the mantle tissue are also induced in the germline --indicating that such changes are potentially heritable. Phase 2 of the project will use a pair-mated cross experiment to test the hypothesis that parental exposure to OA alters larval traits (calcification rate, shell structure, and polymorph mineralogy). Larvae will be generated from parents exposed to OA or control seawater, and then raised under control or OA conditions. Results will be used to (i) characterize inheritance of induced methylation states, (ii) estimate the variance in larval traits explained by genotype, non-genetic maternal/paternal effects, adult OA exposure, larval OA exposure, and parental methylome, and (iii) test the hypothesis that adult exposure alters the heritability (a quantity that predicts evolutionary response) of larval traits. Since the effects of epigenetic phenomena on estimates of heritability are highly debated, the results would advance understanding of this important issue. Because the investigators could discover that DNA methylation is a mechanism for heritable plastic responses to OA, knowledge of this mechanism would significantly improve and potentially transform predictive models for how organisms respond to global change.</p>
Epigenetics to Ocean
largerWorkCitation
project
eng; USA
biota
oceans
2025-03-01
Coastal Massachusetts near Nahant: 42°25'06"N 70°54'14"W
0
BCO-DMO catalogue of parameters from Magnesium isotopic compositions of seawater, extrapallial fluid, and Crassostrea virginica shell material from ocean acidification experiments
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/955181.rdf
Name: Sample _Label
Units: unitless
Description: <p>Sample identification</p>
http://lod.bco-dmo.org/id/dataset-parameter/955182.rdf
Name: pCO2
Units: parts per million (ppm)
Description: <p>Partial pressure of carbon dioxide simulating ocean acidification conditions</p>
http://lod.bco-dmo.org/id/dataset-parameter/955183.rdf
Name: Sample_type
Units: unitless
Description: <p>Identifies which biomineralization reservoir the sample represents</p>
http://lod.bco-dmo.org/id/dataset-parameter/955184.rdf
Name: N
Units: unitless
Description: <p>number???, of what??</p>
http://lod.bco-dmo.org/id/dataset-parameter/955185.rdf
Name: delta_26Mg
Units: per mil
Description: <p>Magnesium isotope ratio</p>
http://lod.bco-dmo.org/id/dataset-parameter/955186.rdf
Name: SD
Units: per mil
Description: <p>Standard deviation of delta 26Mg isotope measurement</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/955164/data/download
download
onLine
dataset
<p>Ocean acidification laboratory experiments were conducted at [____place___] during [____time range___] to examine the effects of ambient (500 ppm CO<sub>2</sub>), moderate (900 ppm CO<sub>2</sub>), and high (2800 ppm CO<sub>2</sub>) ocean acidification conditions on the geochemistry of seawater, extrapallial fluid (EPF) and shells of the Eastern oyster, <em>Crassostrea virginica</em>.&nbsp;</p>
<p><em><strong>[More details are needed here on experimental design, set-up, instruments, etc.]</strong></em></p>
<p>Here we present magnesium isotope data for three biomineralization reservoirs--seawater, EPF, and Crassostrea virginica shells.&nbsp;</p>
<p><em><strong>[More details are needed]</strong></em></p>
<p>Measured on an isotope ratio mass spectrometer [<em><strong>provide make, model, and lab]</strong></em>&nbsp;</p>
Specified by the Principal Investigator(s)
<p dir="ltr">The averages and average standard deviations were found by taking the square root of the squared magnesium isotope values:</p>
<p dir="ltr">SQRT ((CV3 delta-26Mg)^2 +&nbsp;CV6 delta-26Mg)^2&nbsp;+&nbsp;CV7 delta-26Mg)^2&nbsp;+ CV8 delta-26Mg)^2)</p>
<div>&nbsp;</div>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
????
????
PI Supplied Instrument Name: ???? PI Supplied Instrument Description:Need details from submitter Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/