http://lod.bco-dmo.org/id/dataset/955729
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-03-11
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Individual respiration rate measurements for developmental stages of Acartia tonsa during temperature experiments with copepods collected from eastern Long Island Sound in July of 2021
2025-03-11
publication
2025-03-11
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-03-17
publication
https://doi.org/10.26008/1912/bco-dmo.955729.1
Hans G. Dam
University of Connecticut
principalInvestigator
Matthew Sasaki
University of Connecticut
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Holmes-Hackerd, M., Sasaki, M., Dam, H. G. (2025) Individual respiration rate measurements for developmental stages of Acartia tonsa during temperature experiments with copepods collected from eastern Long Island Sound in July of 2021. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-03-11 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.955729.1 [access date]
Methods and Sampling: <p>Acartia tonsa individuals were collected in July from Esker Point, Connecticut in Eastern Long Island Sound in July of 2021. Laboratory cultures were established and maintained at 18°C, with a 12:12 light:dark cycle. To initiate the experiment, nauplii were collected from the laboratory culture and split into two groups, which were either maintained under rearing conditions or exposed 28°C for 24 hours. For the remaining duration of the experiment, all individuals were held at 18°C in individual petri dishes and fed ad libitum a mixture of Tetraselmis sp. and Rhodomonas salina.&nbsp;</p>
<p>Individual copepods were then tracked through development by examining the petri dish daily for the presence of a molted exoskeleton.&nbsp; Respiration rates were measured for each individual starting in the first copepodite stage, with proceeding measurements during each successive stage. During these measurements, individuals were isolated in 2 ml glass vials with an oxygen sensor spot attached to the base. Oxygen levels were measured every three minutes using a Presens sensor dish reader. Assays typically ran for approximately eight hours.&nbsp;Individual lengths were measured using an inverted compound microscope and attached digital camera, and then individuals were returned to their petri dish with fresh medium. Upon reaching maturity (the sixth copepodite stage), respiration and body size was measured as above and then individual sex was recorded.&nbsp;</p>
<p>In addition to the individuals measured at every stage, a group of individuals had measurements made only in the sixth and final stage, serving as a handling control group to test for effects of repeated handling on respiration or body size. The entire experiment (comprising naupliar heat stress, individual measurements through development, and handling control measurements) was repeated three separate times.&nbsp;<br />
<br />
Organism identifiers (Life Science Identifier (LSID))<br />
copepod, Acartia tonsa, urn:lsid:marinespecies.org:taxname:345943<br />
feeding medium,&nbsp;Rhodomonas salina,&nbsp;urn:lsid:marinespecies.org:taxname:106316<br />
feeding medium,&nbsp;Tetraselmis sp.,&nbsp;urn:lsid:marinespecies.org:taxname:134526</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1947965 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1947965
completed
Hans G. Dam
University of Connecticut
860-405-9098
Dept. of Marine Sciences 1080 Shennecossett Road
Groton
CT
06340-6048
USA
hans.dam@uconn.edu
pointOfContact
Matthew Sasaki
University of Connecticut
matthew_sasaki@uml.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
experiment_date
experiment_day
experiment_month
experiment_year
replicate
individual
stage
vial
resp_rate
prosome_length
treatment
group
sex
compound microscope
Presens SDR SensorDish® Reader
theme
None, User defined
date
day of month
month of year
year
replicate
individual
stage
sample description
respiration rate
length
treatment
sex
featureType
BCO-DMO Standard Parameters
Camera
Microscope - Optical
Oxygen Sensor
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Linking eco-evolutionary dynamics of thermal adaptation and grazing in copepods from highly seasonal environments
https://osprey.bco-dmo.org/project/851891
Linking eco-evolutionary dynamics of thermal adaptation and grazing in copepods from highly seasonal environments
<p>NSF Award Abstract:</p>
<p>Many parts of the ocean are warming rapidly, but it is still unknown how this warming will affect marine food webs. Copepods, small crustaceans, are the most abundant animals in the ocean; consequently, they play crucial roles in plankton marine food webs and in the transfer of energy to fishes. Many species of copepods are able to choose between prey such as microscopic plants and single-celled animals. The choice affects how energy moves through marine food webs. Past work suggests that increasing temperature should favor herbivory over carnivory. This project is investigating whether this prediction holds in the face of genetic adaptation to warming in highly seasonal systems such as coastal temperate zones. Results from this study are contributing to understanding and predicting the response of marine ecosystems to future climate conditions, as well as for planning and implementing sustainable fisheries management plans. Other broader impacts include the development of learning modules for high school and college students. Hands-on science exhibits for K-6 students and public presentations at established lecture series focus on the role of copepods in marine food webs in coastal habitats.</p>
<p>Predicting responses of the oceanic biota to climate change is limited not only by an incomplete understanding of how warming affects ecological interactions and evolutionary dynamics individually, but also by how these two factors interact. Copepods are both grazers of phytoplankton and predators of microzooplankton in marine systems. Increasing temperatures may drive a large-scale shift in the diet of omnivorous copepods towards stronger herbivory, with significant consequences for the structure of marine food webs and the control of primary productivity. However, thermal adaptation may moderate or even nullify these shifts. This project examines the interactive role ecological and evolutionary dynamics plays in shaping grazing and individual fitness in a warming ocean. The main goals of the project are to: 1) quantify seasonal variation in thermal performance curves in dominant coastal copepod species; 2) determine whether observed seasonal variation in thermal performance is caused by genetic differentiation or phenotypic plasticity; 3) assess how temperature affects respiration and protein synthesis rates, selective feeding, and individual fitness; and 4) determine how changes in the thermal performance curve, via both genetic differentiation and phenotypic plasticity, affect the relationship between temperature and food preference. Selective feeding experiments are being paired with measurements of egg production and hatching success across a wide range of temperatures to measure thermal effects on feeding selectivity and individual fitness. Finally, genetic differentiation and phenotypic plasticity on temperature sensitivity is being investigated across populations from environments that differ in their thermal regime. The outcomes of this project contribute to the parameterization of models that forecast fisheries dynamics in response to climate change.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
evolutionary_copepods
largerWorkCitation
project
eng; USA
oceans
-71.9996
-71.9996
41.3209
41.3209
2022-03-13
2022-05-20
Connecticut and Florida
0
BCO-DMO catalogue of parameters from Individual respiration rate measurements for developmental stages of Acartia tonsa during temperature experiments with copepods collected from eastern Long Island Sound in July of 2021
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/955847.rdf
Name: experiment_date
Units: unitless
Description: <p>the date respiration rates were measured.</p>
http://lod.bco-dmo.org/id/dataset-parameter/955848.rdf
Name: experiment_day
Units: unitless
Description: <p>the day component of the experiment date.</p>
http://lod.bco-dmo.org/id/dataset-parameter/955849.rdf
Name: experiment_month
Units: unitless
Description: <p>the month component of the experiment date.</p>
http://lod.bco-dmo.org/id/dataset-parameter/955850.rdf
Name: experiment_year
Units: unitless
Description: <p>the year component of the experiment date.</p>
http://lod.bco-dmo.org/id/dataset-parameter/955851.rdf
Name: replicate
Units: unitless
Description: <p>the experimental replicate (recorded as R1, R2, R3 for the first, second, and third experimental replicate respectively).</p>
http://lod.bco-dmo.org/id/dataset-parameter/955852.rdf
Name: individual
Units: unitless
Description: <p>the individual ID for the copepod being measured. The two components of the ID, separated by an underscore, correspond to the replicate (R1, R2, or R3) and the individual number.</p>
http://lod.bco-dmo.org/id/dataset-parameter/955853.rdf
Name: stage
Units: unitless
Description: <p>records the stage each individual was at during the measurement (c1 through c6, indicating the first through sixth copepodite stages).</p>
http://lod.bco-dmo.org/id/dataset-parameter/955854.rdf
Name: vial
Units: unitless
Description: <p>the position of the vial during the oxygen measurements, preceded by the prefix 'Test - ' to differentiate vials from the controls. Vial positions are given by the row (A, B, C, or D) and column (1-6). </p>
http://lod.bco-dmo.org/id/dataset-parameter/955855.rdf
Name: resp_rate
Units: milligrams Oxygen consumed per hour (mg O2/hr)
Description: <p>individual respiration rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/955856.rdf
Name: prosome_length
Units: millimeters (mm)
Description: <p>individual prosome length</p>
http://lod.bco-dmo.org/id/dataset-parameter/955857.rdf
Name: treatment
Units: unitless
Description: <p>either 'heatstress' or 'control', indicating whether an individual was exposed to the 28°C degree heat stress as a nauplii or not.</p>
http://lod.bco-dmo.org/id/dataset-parameter/955858.rdf
Name: group
Units: unitless
Description: <p>either 'active' or 'backup', indicating whether an individual was being actively monitored through development (active group) or was part of the handling control group (backup group).</p>
http://lod.bco-dmo.org/id/dataset-parameter/955859.rdf
Name: sex
Units: unitless
Description: <p>the sex of the individual, identified at the adult stage. Sex is recorded as "M" for male individuals, "F" for female individuals, or "NA" when the sex was unable to be determined because the individual did not survive to the adult stage.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
21754
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/b873d462-c296-4b9a-9001-5f0f5973b28a/content
download
https://doi.org/10.26008/1912/bco-dmo.955729.1
download
onLine
dataset
<p>Acartia tonsa individuals were collected in July from Esker Point, Connecticut in Eastern Long Island Sound in July of 2021. Laboratory cultures were established and maintained at 18°C, with a 12:12 light:dark cycle. To initiate the experiment, nauplii were collected from the laboratory culture and split into two groups, which were either maintained under rearing conditions or exposed 28°C for 24 hours. For the remaining duration of the experiment, all individuals were held at 18°C in individual petri dishes and fed ad libitum a mixture of Tetraselmis sp. and Rhodomonas salina.&nbsp;</p>
<p>Individual copepods were then tracked through development by examining the petri dish daily for the presence of a molted exoskeleton.&nbsp; Respiration rates were measured for each individual starting in the first copepodite stage, with proceeding measurements during each successive stage. During these measurements, individuals were isolated in 2 ml glass vials with an oxygen sensor spot attached to the base. Oxygen levels were measured every three minutes using a Presens sensor dish reader. Assays typically ran for approximately eight hours.&nbsp;Individual lengths were measured using an inverted compound microscope and attached digital camera, and then individuals were returned to their petri dish with fresh medium. Upon reaching maturity (the sixth copepodite stage), respiration and body size was measured as above and then individual sex was recorded.&nbsp;</p>
<p>In addition to the individuals measured at every stage, a group of individuals had measurements made only in the sixth and final stage, serving as a handling control group to test for effects of repeated handling on respiration or body size. The entire experiment (comprising naupliar heat stress, individual measurements through development, and handling control measurements) was repeated three separate times.&nbsp;<br />
<br />
Organism identifiers (Life Science Identifier (LSID))<br />
copepod, Acartia tonsa, urn:lsid:marinespecies.org:taxname:345943<br />
feeding medium,&nbsp;Rhodomonas salina,&nbsp;urn:lsid:marinespecies.org:taxname:106316<br />
feeding medium,&nbsp;Tetraselmis sp.,&nbsp;urn:lsid:marinespecies.org:taxname:134526</p>
Specified by the Principal Investigator(s)
<p>Respiration rates were calculated as the linear change in oxygen concentration over time (milliliter oxygen per hour). The code utilized for these calculations is included in the referenced Zenodo holding (doi: 10.5281/ZENODO.7434983).&nbsp;</p>
Specified by the Principal Investigator(s)
* Data from submitted file "holmes-hackerd et al 2022 data.csv" was imported into the BCO-DMO data system for this dataset. Values "NA" imported as missing data values. Table will appear as Data File: 955729_v1_a-tonsa-ontogenetic-resipration.csv (along with other download format options).
Missing Data Identifiers:
* In the BCO-DMO data system missing data identifiers are displayed according to the format of data you access. For example, in csv files it will be blank (null) values. In Matlab .mat files it will be NaN values. When viewing data online at BCO-DMO, the missing value will be shown as blank (null) values.
* Date converted to ISO 8601 format
* Organism LSIDs added from matches at the World Register of Marine Species (WoRMS) on 2025-03-11
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:Individual lengths were measured using an inverted compound microscope and attached digital camera Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/
compound microscope
compound microscope
PI Supplied Instrument Name: compound microscope PI Supplied Instrument Description:Individual lengths were measured using an inverted compound microscope and attached digital camera Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
Presens SDR SensorDish® Reader
Presens SDR SensorDish® Reader
PI Supplied Instrument Name: Presens SDR SensorDish® Reader PI Supplied Instrument Description:Oxygen consumption was measured using a Presens SDR SensorDish® Reader. Instrument Name: Oxygen Sensor Instrument Short Name:Dissolved Oxygen Sensor Instrument Description: An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed