http://lod.bco-dmo.org/id/dataset/955733
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-03-11
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Individual thermal limit measurements for Acartia tonsa during Critical Thermal Maxima (CTmax) experiments under different feeding treatments with copepods collected from eastern Long Island Sound in July 2020
2025-03-11
publication
2025-03-11
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-03-17
publication
https://doi.org/10.26008/1912/bco-dmo.955733.1
Hans G. Dam
University of Connecticut
principalInvestigator
Matthew Sasaki
University of Connecticut
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Rueda-Moreno, G., Sasaki, M., Dam, H. G. (2025) Individual thermal limit measurements for Acartia tonsa during Critical Thermal Maxima (CTmax) experiments under different feeding treatments with copepods collected from eastern Long Island Sound in July 2020. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-03-11 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.955733.1 [access date]
Methods and Sampling: <p>Copepods were collected using a 250-um mesh plankton net with a solid cod end and then cultured in the laboratory for several generations. Adult female copepods from the laboratory culture were collected and split into two groups, one fed ad libitum on Tetraselmis sp. and one provided with no food.&nbsp;&nbsp;Upper thermal limits were then measured daily as the critical thermal maximum (CTmax) of individual copepods, or the temperature at which normal behavior ceases. CTmax measurements were made for individuals in both treatments for five days. These measurements are made during a gradual temperature ramp (at a rate of 0.1-0.3°C per minute). The CTmax methodology is described in Sasaki et al. 2023. Copepods are placed into 50 ml flat-bottom glass vials, which are immersed in a water bath. Temperatures are gradually increased using a 300 watt aquarium heater. Individual copepods are monitored throughout the temperature ramp until movement ceases, indicating the individual has reached its CTmax. The water temperature at that point is recovered from a continuous temperature record; three sensors are placed into separate vials in the water bath and record temperature every five seconds for the duration of the experiment.&nbsp;<br />
<br />
Organism identifiers (Life Science Identifier (LSID))<br />
copepod, Acartia tonsa, urn:lsid:marinespecies.org:taxname:345943<br />
feeding medium, Tetraselmis sp., urn:lsid:marinespecies.org:taxname:134526</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1947965 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1947965
completed
Hans G. Dam
University of Connecticut
860-405-9098
Dept. of Marine Sciences 1080 Shennecossett Road
Groton
CT
06340-6048
USA
hans.dam@uconn.edu
pointOfContact
Matthew Sasaki
University of Connecticut
matthew_sasaki@uml.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
replicate
experiment_date
experiment_day
experiment_month
experiment_year
diet
tube
ctmax
length
300-watt aquarium heater
MCP9808-E/MS temperature sensors from Microchips Technology (MCP9808-E/MS)
theme
None, User defined
replicate
date
day of month
month of year
year
treatment
tube number
No BCO-DMO term
length
featureType
BCO-DMO Standard Parameters
Immersion heater
Water Temperature Sensor
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Linking eco-evolutionary dynamics of thermal adaptation and grazing in copepods from highly seasonal environments
https://osprey.bco-dmo.org/project/851891
Linking eco-evolutionary dynamics of thermal adaptation and grazing in copepods from highly seasonal environments
<p>NSF Award Abstract:</p>
<p>Many parts of the ocean are warming rapidly, but it is still unknown how this warming will affect marine food webs. Copepods, small crustaceans, are the most abundant animals in the ocean; consequently, they play crucial roles in plankton marine food webs and in the transfer of energy to fishes. Many species of copepods are able to choose between prey such as microscopic plants and single-celled animals. The choice affects how energy moves through marine food webs. Past work suggests that increasing temperature should favor herbivory over carnivory. This project is investigating whether this prediction holds in the face of genetic adaptation to warming in highly seasonal systems such as coastal temperate zones. Results from this study are contributing to understanding and predicting the response of marine ecosystems to future climate conditions, as well as for planning and implementing sustainable fisheries management plans. Other broader impacts include the development of learning modules for high school and college students. Hands-on science exhibits for K-6 students and public presentations at established lecture series focus on the role of copepods in marine food webs in coastal habitats.</p>
<p>Predicting responses of the oceanic biota to climate change is limited not only by an incomplete understanding of how warming affects ecological interactions and evolutionary dynamics individually, but also by how these two factors interact. Copepods are both grazers of phytoplankton and predators of microzooplankton in marine systems. Increasing temperatures may drive a large-scale shift in the diet of omnivorous copepods towards stronger herbivory, with significant consequences for the structure of marine food webs and the control of primary productivity. However, thermal adaptation may moderate or even nullify these shifts. This project examines the interactive role ecological and evolutionary dynamics plays in shaping grazing and individual fitness in a warming ocean. The main goals of the project are to: 1) quantify seasonal variation in thermal performance curves in dominant coastal copepod species; 2) determine whether observed seasonal variation in thermal performance is caused by genetic differentiation or phenotypic plasticity; 3) assess how temperature affects respiration and protein synthesis rates, selective feeding, and individual fitness; and 4) determine how changes in the thermal performance curve, via both genetic differentiation and phenotypic plasticity, affect the relationship between temperature and food preference. Selective feeding experiments are being paired with measurements of egg production and hatching success across a wide range of temperatures to measure thermal effects on feeding selectivity and individual fitness. Finally, genetic differentiation and phenotypic plasticity on temperature sensitivity is being investigated across populations from environments that differ in their thermal regime. The outcomes of this project contribute to the parameterization of models that forecast fisheries dynamics in response to climate change.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
evolutionary_copepods
largerWorkCitation
project
eng; USA
oceans
-72.001655
-72.001655
41.32074
41.32074
2022-07-10
2022-10-28
Connecticut and Florida
0
BCO-DMO catalogue of parameters from Individual thermal limit measurements for Acartia tonsa during Critical Thermal Maxima (CTmax) experiments under different feeding treatments with copepods collected from eastern Long Island Sound in July 2020
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/955896.rdf
Name: replicate
Units: unitless
Description: <p>The experimental replicate (1 through 5)</p>
http://lod.bco-dmo.org/id/dataset-parameter/955897.rdf
Name: experiment_date
Units: unitless
Description: <p>The date thermal limits were measured</p>
http://lod.bco-dmo.org/id/dataset-parameter/955898.rdf
Name: experiment_day
Units: unitless
Description: <p>The day component of the experiment date</p>
http://lod.bco-dmo.org/id/dataset-parameter/955899.rdf
Name: experiment_month
Units: units
Description: <p>The month component of the experiment date</p>
http://lod.bco-dmo.org/id/dataset-parameter/955900.rdf
Name: experiment_year
Units: unitless
Description: <p>The year component of the experiment date</p>
http://lod.bco-dmo.org/id/dataset-parameter/955901.rdf
Name: diet
Units: unitless
Description: <p>Whether individuals were from the fed or starved treatments</p>
http://lod.bco-dmo.org/id/dataset-parameter/955902.rdf
Name: tube
Units: unitless
Description: <p>The tube position during the experiment (positions 1 through 10)</p>
http://lod.bco-dmo.org/id/dataset-parameter/955903.rdf
Name: ctmax
Units: degrees Celsius
Description: <p>The measured critical thermal maximum for the individual</p>
http://lod.bco-dmo.org/id/dataset-parameter/955904.rdf
Name: length
Units: millimeters (mm)
Description: <p>The measured prosome length of the individual</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
13266
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/f37684ec-301b-4ceb-85db-8c0f12db17fa/content
download
https://doi.org/10.26008/1912/bco-dmo.955733.1
download
onLine
dataset
<p>Copepods were collected using a 250-um mesh plankton net with a solid cod end and then cultured in the laboratory for several generations. Adult female copepods from the laboratory culture were collected and split into two groups, one fed ad libitum on Tetraselmis sp. and one provided with no food.&nbsp;&nbsp;Upper thermal limits were then measured daily as the critical thermal maximum (CTmax) of individual copepods, or the temperature at which normal behavior ceases. CTmax measurements were made for individuals in both treatments for five days. These measurements are made during a gradual temperature ramp (at a rate of 0.1-0.3°C per minute). The CTmax methodology is described in Sasaki et al. 2023. Copepods are placed into 50 ml flat-bottom glass vials, which are immersed in a water bath. Temperatures are gradually increased using a 300 watt aquarium heater. Individual copepods are monitored throughout the temperature ramp until movement ceases, indicating the individual has reached its CTmax. The water temperature at that point is recovered from a continuous temperature record; three sensors are placed into separate vials in the water bath and record temperature every five seconds for the duration of the experiment.&nbsp;<br />
<br />
Organism identifiers (Life Science Identifier (LSID))<br />
copepod, Acartia tonsa, urn:lsid:marinespecies.org:taxname:345943<br />
feeding medium, Tetraselmis sp., urn:lsid:marinespecies.org:taxname:134526</p>
Specified by the Principal Investigator(s)
<p>The raw data and code used to measure CTmax are contained in the referenced GitHub repository&nbsp;https://github.com/ZoopEcoEvo/starved_ctmax (archival copy "ZoopEcoEvo/starved_ctmax: Initial release" doi:&nbsp;10.5281/zenodo.8057949).</p>
Specified by the Principal Investigator(s)
* Data from submitted file "Rueda-Moreno and Sasaki 2023.csv" was imported into the BCO-DMO data system for this dataset. Values "NA" imported as missing data values. Table will appear as Data File: 955733_v1_a-tonsa-ctmax-feeding-exp.csv (along with other download format options).
Missing Data Identifiers:
* In the BCO-DMO data system missing data identifiers are displayed according to the format of data you access. For example, in csv files it will be blank (null) values. In Matlab .mat files it will be NaN values. When viewing data online at BCO-DMO, the missing value will be shown as blank (null) values.
* Date converted to ISO 8601 format
* Organism LSIDs added from matches at the World Register of Marine Species (WoRMS) on 2025-03-11
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
300-watt aquarium heater
300-watt aquarium heater
PI Supplied Instrument Name: 300-watt aquarium heater Instrument Name: Immersion heater Instrument Short Name:Immersion heater Instrument Description: Submersible heating element for water tanks and aquaria.
MCP9808-E/MS temperature sensors from Microchips Technology (MCP9808-E/MS)
MCP9808-E/MS temperature sensors from Microchips Technology (MCP9808-E/MS)
PI Supplied Instrument Name: MCP9808-E/MS temperature sensors from Microchips Technology (MCP9808-E/MS) PI Supplied Instrument Description:MCP9808-E/MS temperature sensors from Microchips Technology (MCP9808-E/MS) were used during the CTmax experiments. Instrument Name: Water Temperature Sensor Instrument Short Name:Water Temp Sensor Instrument Description: General term for an instrument that measures the temperature of the water with which it is in contact (thermometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/134/