http://lod.bco-dmo.org/id/dataset/955739
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-03-11
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Upper thermal limits (LD50) for two common coastal copepods during thermal experiments with individuals collected from Long Island Sound between July 2017 and November 2019
2025-03-11
publication
2025-03-11
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-03-17
publication
https://doi.org/10.26008/1912/bco-dmo.955739.1
Hans G. Dam
University of Connecticut
principalInvestigator
Matthew Sasaki
University of Connecticut
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Sasaki, M., Dam, H. G. (2025) Upper thermal limits (LD50) for two common coastal copepods during thermal experiments with individuals collected from Long Island Sound between July 2017 and November 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-03-11 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.955739.1 [access date]
Methods and Sampling: <p>Copepods were collected using a 250-um mesh plankton net with a solid cod end. Upper thermal limits were measured as LD50, or the temperature at which only 50% of individuals survived after a 24-hour acute heat shock, estimated from a thermal survivorship curve. These curves were generated by exposing individual copepods to a static temperature for 24 hours. Each individual was placed in a 2 mL microcentrifuge tube with 1.5 mL of 0.2 um filtered seawater. These tubes were then moved to 15-well dry baths, set to temperatures between 10°C and 39°C. Each individual experienced only one temperature. After the 24 hour period, survival for each individual was checked. This binary survival data was then used to generate survivorship curves for each collection using a logistic regression. LD50 was then extracted from these curves.&nbsp;<br />
<br />
Organism identifiers (Life Science Identifier (LSID))<br />
copepod, Acartia tonsa, urn:lsid:marinespecies.org:taxname:345943<br />
copepod, Acartia (Acartiura) hudsonica,&nbsp;urn:lsid:marinespecies.org:taxname:149751</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1947965 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1947965
completed
Hans G. Dam
University of Connecticut
860-405-9098
Dept. of Marine Sciences 1080 Shennecossett Road
Groton
CT
06340-6048
USA
hans.dam@uconn.edu
pointOfContact
Matthew Sasaki
University of Connecticut
matthew_sasaki@uml.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
species
acclimation
collection_id
collection_date
collection_month
collection_year
collection_temp
acclimation_temp
LD50
SE
15-well drybaths from USA Scientific
theme
None, User defined
species_epithet
sample description
date
month of year
year
water temperature at measurement depth
standard error
featureType
BCO-DMO Standard Parameters
Incubator
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Linking eco-evolutionary dynamics of thermal adaptation and grazing in copepods from highly seasonal environments
https://osprey.bco-dmo.org/project/851891
Linking eco-evolutionary dynamics of thermal adaptation and grazing in copepods from highly seasonal environments
<p>NSF Award Abstract:</p>
<p>Many parts of the ocean are warming rapidly, but it is still unknown how this warming will affect marine food webs. Copepods, small crustaceans, are the most abundant animals in the ocean; consequently, they play crucial roles in plankton marine food webs and in the transfer of energy to fishes. Many species of copepods are able to choose between prey such as microscopic plants and single-celled animals. The choice affects how energy moves through marine food webs. Past work suggests that increasing temperature should favor herbivory over carnivory. This project is investigating whether this prediction holds in the face of genetic adaptation to warming in highly seasonal systems such as coastal temperate zones. Results from this study are contributing to understanding and predicting the response of marine ecosystems to future climate conditions, as well as for planning and implementing sustainable fisheries management plans. Other broader impacts include the development of learning modules for high school and college students. Hands-on science exhibits for K-6 students and public presentations at established lecture series focus on the role of copepods in marine food webs in coastal habitats.</p>
<p>Predicting responses of the oceanic biota to climate change is limited not only by an incomplete understanding of how warming affects ecological interactions and evolutionary dynamics individually, but also by how these two factors interact. Copepods are both grazers of phytoplankton and predators of microzooplankton in marine systems. Increasing temperatures may drive a large-scale shift in the diet of omnivorous copepods towards stronger herbivory, with significant consequences for the structure of marine food webs and the control of primary productivity. However, thermal adaptation may moderate or even nullify these shifts. This project examines the interactive role ecological and evolutionary dynamics plays in shaping grazing and individual fitness in a warming ocean. The main goals of the project are to: 1) quantify seasonal variation in thermal performance curves in dominant coastal copepod species; 2) determine whether observed seasonal variation in thermal performance is caused by genetic differentiation or phenotypic plasticity; 3) assess how temperature affects respiration and protein synthesis rates, selective feeding, and individual fitness; and 4) determine how changes in the thermal performance curve, via both genetic differentiation and phenotypic plasticity, affect the relationship between temperature and food preference. Selective feeding experiments are being paired with measurements of egg production and hatching success across a wide range of temperatures to measure thermal effects on feeding selectivity and individual fitness. Finally, genetic differentiation and phenotypic plasticity on temperature sensitivity is being investigated across populations from environments that differ in their thermal regime. The outcomes of this project contribute to the parameterization of models that forecast fisheries dynamics in response to climate change.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
evolutionary_copepods
largerWorkCitation
project
eng; USA
oceans
-72.001655
-72.001655
41.32074
41.32074
2017-05-07
2019-11-17
Connecticut and Florida
0
BCO-DMO catalogue of parameters from Upper thermal limits (LD50) for two common coastal copepods during thermal experiments with individuals collected from Long Island Sound between July 2017 and November 2019
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/955992.rdf
Name: species
Units: unitless
Description: <p>"tonsa" or "hudsonica", referring to the species thermal tolerance was measured for (Acartia tonsa and Acartia hudsonica, respectively)</p>
http://lod.bco-dmo.org/id/dataset-parameter/955993.rdf
Name: acclimation
Units: unitless
Description: <p>whether copepods were acclimated to field- or laboratory conditions prior to thermal tolerance measurements. </p>
http://lod.bco-dmo.org/id/dataset-parameter/955994.rdf
Name: collection_id
Units: unitless
Description: <p> the collection ID. For field-acclimated copepods this is the date of collection, while for laboratory-acclimated copepods this is the culture ID.</p>
http://lod.bco-dmo.org/id/dataset-parameter/955995.rdf
Name: collection_date
Units: unitless
Description: <p>collection date</p>
http://lod.bco-dmo.org/id/dataset-parameter/955996.rdf
Name: collection_month
Units: unitless
Description: <p>collection month</p>
http://lod.bco-dmo.org/id/dataset-parameter/955997.rdf
Name: collection_year
Units: unitless
Description: <p>collection year</p>
http://lod.bco-dmo.org/id/dataset-parameter/955998.rdf
Name: collection_temp
Units: degrees Celsius
Description: <p>the water temperature recorded at the time of collection. degrees Celsius.</p>
http://lod.bco-dmo.org/id/dataset-parameter/955999.rdf
Name: acclimation_temp
Units: degrees Celsius
Description: <p>the temperature copepods were acclimated to prior to thermal tolerance measurements. This is equivalent to collection-temperature for field-acclimated copepods, while for laboratory-acclimated copepods indicates the static temperature they were reared at.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956000.rdf
Name: LD50
Units: degrees Celsius
Description: <p>upper thermal limit for each collection, measured as the temperature where 50% mortality is observed.</p>
http://lod.bco-dmo.org/id/dataset-parameter/956001.rdf
Name: SE
Units: degrees Celsius
Description: <p> standard error estimate for the LD50 measurement. </p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2540
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/f84d5742-fb1e-4f0d-ac10-275b9d182292/content
download
https://doi.org/10.26008/1912/bco-dmo.955739.1
download
onLine
dataset
<p>Copepods were collected using a 250-um mesh plankton net with a solid cod end. Upper thermal limits were measured as LD50, or the temperature at which only 50% of individuals survived after a 24-hour acute heat shock, estimated from a thermal survivorship curve. These curves were generated by exposing individual copepods to a static temperature for 24 hours. Each individual was placed in a 2 mL microcentrifuge tube with 1.5 mL of 0.2 um filtered seawater. These tubes were then moved to 15-well dry baths, set to temperatures between 10°C and 39°C. Each individual experienced only one temperature. After the 24 hour period, survival for each individual was checked. This binary survival data was then used to generate survivorship curves for each collection using a logistic regression. LD50 was then extracted from these curves.&nbsp;<br />
<br />
Organism identifiers (Life Science Identifier (LSID))<br />
copepod, Acartia tonsa, urn:lsid:marinespecies.org:taxname:345943<br />
copepod, Acartia (Acartiura) hudsonica,&nbsp;urn:lsid:marinespecies.org:taxname:149751</p>
Specified by the Principal Investigator(s)
<p>Thermal tolerance (LD50) values were extracted from logistic regressions of individual survival against stress temperature. All raw data and code associated with these analyses are available from a Dryad repository:&nbsp;https://doi.org/10.5061/dryad.9kd51c5dg&nbsp;</p>
Specified by the Principal Investigator(s)
* Data from submitted file "Sasaki Dam 2020 LD50_bcodmo-edit_MS-response.xlsx" was imported into the BCO-DMO data system for this dataset. Values "NA" imported as missing data values. Table will appear as Data File: 955739_v1_a-tonsa-hudsonica-thermal-limits.csv (along with other download format options).
Missing Data Identifiers:
* In the BCO-DMO data system missing data identifiers are displayed according to the format of data you access. For example, in csv files it will be blank (null) values. In Matlab .mat files it will be NaN values. When viewing data online at BCO-DMO, the missing value will be shown as blank (null) values.
* Organism LSIDs added from matches at the World Register of Marine Species (WoRMS) on 2025-03-11.
Note, "Acartia hudsonica Pinhey, 1926" (urn:lsid:marinespecies.org:taxname:157664) is classified as an "alternate representation" of the currently accepted name "Acartia (Acartiura) hudsonica Pinhey, 1926" (urn:lsid:marinespecies.org:taxname:149751). The accepted name LSID was added to the Methods & Sampling metadata.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
15-well drybaths from USA Scientific
15-well drybaths from USA Scientific
PI Supplied Instrument Name: 15-well drybaths from USA Scientific PI Supplied Instrument Description:The 24 hour acute heat stress used 15-well drybaths from USA Scientific. Instrument Name: Incubator Instrument Short Name:incubator (general) Instrument Description: A device in which environmental conditions (light, photoperiod, temperature, humidity, etc.) can be controlled.
Note: we have more specific terms for shipboard incubators (https://www.bco-dmo.org/instrument/629001) and in-situ incubators (https://www.bco-dmo.org/instrument/494).