1b:I[42426,["/_next/static/immutable/chunks/1xctgzqre-wsy.js","/_next/static/immutable/chunks/06_-wz6pdtp2b.js","/_next/static/immutable/chunks/30e52g49hg40p.js","/_next/static/immutable/chunks/02yq6tul2z-oh.js","/_next/static/immutable/chunks/10bx_ec2vj7n_.js","/_next/static/immutable/chunks/33bfn03fo8xnt.js","/_next/static/immutable/chunks/0tfjf78scu61b.js","/_next/static/immutable/chunks/31xnvej4n1bi9.js"],"default"] 1c:T1463,

Mesocosm incubations:

For mesocosm (large volume) incubation experiments (referred to as “LV” incubations), seawater was transferred to 20 L carboys that were rinsed three times with water from the sampling depth and then filled with seawater from a single Niskin bottle, using silicone tubing that had been acid washed then rinsed with distilled water prior to use. Four carboys were filled from bottom water and deep chlorophyll maximum (DCM) water each, according to the CTD. Triplicate 20L carboys (a total of 9 carboys) were amended with high molecular weight material isolated from the diatom Thalassiosira, or the polysaccharide fucodian, or the polysaccharide arabinogalactan; unamended single carboys were used for controls. From each carboy, water was dispensed into smaller glass containers that were cleaned and pre-rinsed three times with water from the carboy prior to dispensing. This water was used to measure the activities of peptidases, and glucosidases. A separate glass Duran bottle was filled with seawater from the carboy and sterilized in an autoclave to serve as a killed control for microbial activity measurements. All mesocosms were incubated in the dark at near in-situ temperatures. Mesocosms were sub-sampled for peptidase and glucosidase activity measurements at the start of incubation (0 days), and then after at approximately 5d, 10d, 15d, and 25d. To measure gluosidase and peptidase activities at each timepoint, water was collected from the mesocosms as described above.

For these measurements, seven glucosidase and peptidase substrates were set up in a 96-well plate. The substrates used included alpha-glucose and beta-glucose linked to a 4-methylumbelliferyl (MUF) fluorophore to measure exo-acting glucosidase activities. Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, including one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and phenylalanine-serine-arginine (FSR) – were used to measure exo- and endo-acting peptidase activities, respectively. For each substrate, triplicate wells were filled with a total volume of 200 uL seawater for experimental incubations; triplicate wells were filled with 200 uL autoclaved seawater for killed control incubations. Substrate was added at saturating concentrations. A saturation curve was determined with surface water from each station to identify saturating concentrations of substrate. The saturating concentration was identified as the lowest tested concentration of substrate at which additional substrate did not yield higher rates of hydrolysis. Fluorescence was measured over 24-48 hours incubation time with a plate reader (TECAN infiniteF200; 360 nm excitation, 460 emission), with timepoints taken every 4-6 hours. Bottom water measurements were made in a cold van/cold room at 4 C; water from the deep chlorophyll maximum was incubated at room temperature.

Bulk water incubations:

Seawater was transferred to 20 L carboys that were rinsed three times with water from the sampling depth and then filled with seawater from a single Niskin bottle, using silicone tubing that had been acid washed then rinsed with distilled water prior to use. From each carboy, water was dispensed into smaller glass containers that were cleaned and pre-rinsed three times with water from the carboy prior to dispensing. This water was used to measure the activities of peptidases, and glucosidases. A separate glass Duran bottle was filled with seawater from the carboy and sterilized in an autoclave for 20-30 minutes to serve as a killed control for microbial activity measurements.Two substrates, alpha-glucose and beta-glucose linked to a 4-methylumbelliferyl (MUF) fluorophore, were used to measure glucosidase activities. Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and phenylalanine-serine-arginine (FSR) – were used to measure exo- and endo-acting peptidase activities, respectively. Incubations with the seven low molecular weight substrates were set up in a 96-well plate. For each substrate, triplicate wells were filled with a total volume of 200 uL seawater for experimental incubations; triplicate wells were filled with 200 uL autoclaved seawater for killed control incubations. Substrate was added at saturating concentrations. A saturation curve was determined with surface water from each station to identify saturating concentrations of substrate.The saturating concentration was identified as the lowest tested concentration of substrate at which additional substrate did not yield higher rates of hydrolysis. Fluorescence was measured over 0-72 hours incubation time with a plate reader (TECAN infiniteF200; 360 nm excitation, 460 emission), with timepoints taken every 4-6 hours.

1d:T8b7,This dataset includes the measurements of leucine aminopeptidase, glucosidase, and endo-acting (mid-chain cleaving) peptidase activities from bulk and mesocosm incubations from waters taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 (2022-05-24 to 2022-06-12). Waters for bulk incubation were taken at three sites and various depths, mesocosm incubations were taken at two sites and two depths. This research tested the hypothesis that the mechanism of polysaccharide processing is related to the cost to a cell of producing the enzymes required for its hydrolysis, and the probability that a cell will receive sufficient return on investment for producing the enzymes. Our conceptual model suggests that external (extracellular) hydrolysis is favored when organic matter is abundant, or when enzyme production costs can be shared (e.g., on particles, in biofilms); selfish uptake (hydrolysis without production of low molecular weight products in the environment) would be a better strategy when high molecular weight (HMW) organic matter is scarce, and particularly when the HMW organic matter is very complex. Seawater was sampled from four depths at our initial station, and the deep chlorophyll maximum and bottom water from two subsequent stations differing in typical extent of primary productivity. We incubated mesocosms of seawater from the deep chlorophyll maximum and from bottom water from two stations differing in typical extent of primary productivity. Organic matter availability was amended by adding high molecular weight organic matter (dissolved and particulate) from diatoms, or by adding the polysaccharide fucoidan, or by adding the polysaccharide arabinogalactan. Amendments were performed in triplicate mesocosms, while one mesocosm was left unamended for a total of ten mesocosms from each depth and station tested These data help test this model because they provide information about the activities of exo-acting glucosidases and an exo-acting peptidase, plus endo-acting peptidases, all of which are used to hydrolyze high molecular weight organic matter. Activities of these enzymes differed by station as well as by depth1e:T46a,Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.1f:Tcb7,Substrate Structural Complexity and Abundance Control Distinct Mechanisms of Microbially-Driven Carbon Cycling in the Ocean Almost half of the organic carbon produced in the ocean is processed by bacteria. Bacteria use extracellular (outside the cell) enzymes to break down large organic molecules to small sizes that can be transported into their cells. It has recently been discovered that bacteria use extracellular enzymes in two ways: ‘selfish uptake’ and ‘external hydrolysis’. External hydrolysis releases low molecular weight products to the environment where they can be used by other organisms. ‘Selfish uptake’ releases little or no products. This research will determine the extent and location of ‘selfish uptake’ in ocean waters. This process affects the distribution of organic carbon in the ocean, the flow of small organic molecules to feed a wider range of bacteria, and the composition and dynamics of the bacterial community. Recent results show that ‘selfish’ bacteria are active in deep ocean waters, where they take up complex polysaccharides (sugars) that are not hydrolyzed externally. These results inspired a new model that links ‘selfish uptake’ and external hydrolysis to the amount and complexity of the organic matter that is used by bacteria. This project will test the model by describing the polysaccharide fraction of marine organic matter, and studying the relationships between organic matter abundance, structural complexity, and extracellular enzyme use. Graduate and undergraduate students will participate in the project as members of the research team in the field and in the laboratory. This research will test the hypothesis that the mechanism of polysaccharide processing is related to the cost to a cell of producing the enzymes required for its hydrolysis, and the probability that a cell will receive sufficient return on investment for producing the enzymes. The conceptual model that will be tested suggests that external hydrolysis is favored when organic matter is abundant, or when enzyme production costs can be shared (e.g., on particles, in biofilms); selfish uptake would be a better strategy when high molecular weight (HMW) organic matter is scarce, and particularly when the HMW organic matter is very complex. This study will test this model by characterizing the structure of polysaccharide-containing components of dissolved organic matter (DOM) and particulate organic matter (POM) collected from the ocean, by determining the extent of selfish uptake and rates of external hydrolysis of different polysaccharides by natural microbial communities from the surface and the deep ocean, and by incubation experiments that control for the abundance of polysaccharides of different structural complexity. This project will be carried out in collaboration with colleagues at the Max Planck Institute for Marine Microbiology, whose expertise in carbohydrate chemistry and structural analyses, and in advanced microscopy and analysis of complex microbial communities, are central to the project. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.6:["$","$L1b",null,{"dataset":{"_id":"956085","description":"","problem":"","acquisition":"$1c","processing":"

Hydrolysis of the substrates was measured as an increase in fluorescence as the fluorophore was hydrolyzed from the substrate over time [as in Hoppe, 1983; Obayashi and Suzuki, 2005].

Hydrolysis rates were calculated from the rate of increase of fluorescence in the incubation over time relative to a set of standards of known concentration of fluorophore. Calculations followed the procedure outlined in the tutorial available in the associated Github repository (https://github.com/ArnostiLab/ArnostiLab-RScript-Demo-PlateRdr/tree/master/scripts (DOI: 10.5281/zenodo.14783119).

\n","bcodmoProcessing":"* Merged bulk water and mesocosm incubations data into 1 dataset (added Sample_type parameter to differentiate between the 2 datasets)\r\n* Added ISO_DateTime UTC variable to data\r\n* Merged bulk water and mesocosm incubations metadata into 1 landing page\r\n* ","title":"Peptidase and glucosidase activities from mesocosm and bulk water incubations from waters taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022.","nickname":"Peptidase and glucosidase activities from mesocosm and bulk water incubations.","dataUrl":"","currentState":"Final no updates expected","currentStateId":"7","validated":true,"restricted":false,"version":"1","version_date":"2025-03-17T04:00:00.000Z","abstract":"$1d","citation":"Lloyd, C., Ghobrial, S., Arnosti, C. (2025) Peptidase and glucosidase activities from mesocosm and bulk water incubations from waters taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-03-17 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.956085.1 [access date]","citationParts":{"identifier":"doi:10.26008/1912/bco-dmo.956085.1","versionDate":"2025-03-17","versionId":"1","year":"2025","title":"Peptidase and glucosidase activities from mesocosm and bulk water incubations from waters taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022.","authors":"Lloyd, C., Ghobrial, S., Arnosti, C.","publisher":"Biological and Chemical Oceanography Data Management Office (BCO-DMO)."},"doi":"10.26008/1912/bco-dmo.956085.1","release_date":null,"field_dataset_location":"Western North Atlantic","temporal_extent":{"start":"2022-05-22T04:00:00.000Z","end":"2022-06-09T04:00:00.000Z"},"temporal":{"resolution":"time:unitDay","type":"time:Interval","start":{"value":"1653192000","label":"2022-05-22"},"end":{"value":"1654747200","label":"2022-06-09"}},"spatial_extent":{"shape":"polygon","center":{"latitude":38.3071298,"longitude":-64.5851255},"wkt":"POLYGON ((-75.296666 33.7441666, -53.873585 33.7441666, -53.873585 42.870093, -75.296666 42.870093, -75.296666 33.7441666))","north":42.870093,"east":-53.873585,"south":33.7441666,"west":-75.296666},"dataset_type":["Other Field Results"],"keywords":["oceans"],"data_files":[{"id":"967133","title":"956085_v1_peptidase.csv","url":"https://datadocs.bco-dmo.org/dataset/956085/file/vmy7o1XiNDgYq/956085_v1_peptidase.csv","showTableUrl":"https://www.bco-dmo.org/dataset/956085/data/view/967133","description":"Primary data file for dataset ID 956085, version 1","filename":"956085_v1_peptidase.csv","bytesize":281090,"checksum":{"value":"67a1cca8ac8dce5a3e798a0ca97c89cb","Algorithm":{"_id":"190","name":"MD5"}},"mimetype":"text/csv","fileType":{"_id":"192","name":"Comma Separated Values (.csv)"},"isErddap":"","date":"","isPublicDuringEmbargo":false,"primaryDataFile":true,"showTable":true,"restricted":false}],"supplemental_files":[],"roles":[{"role":{"_id":"138","name":"Principal Investigator"},"performedBy":{"_id":"661940","name":"Carol Arnosti"},"performedAtOrganization":{"_id":"20","name":"University of North Carolina at Chapel Hill"}},{"role":{"_id":"128","name":"Student"},"performedBy":{"_id":"956112","name":"Chad Lloyd"},"performedAtOrganization":{"_id":"20","name":"University of North Carolina at Chapel Hill"}},{"role":{"_id":"133","name":"Contact"},"performedBy":{"_id":"890958","name":"Sherif Ghobrial"},"performedAtOrganization":{"_id":"20","name":"University of North Carolina at Chapel Hill"}},{"role":{"_id":"137","name":"Data Manager"},"performedBy":{"_id":"890958","name":"Sherif Ghobrial"},"performedAtOrganization":{"_id":"20","name":"University of North Carolina at Chapel Hill"}},{"role":{"_id":"145","name":"BCO-DMO Data Manager"},"performedBy":{"_id":"748773","name":"Karen Soenen"},"performedAtOrganization":{"_id":"191","name":"Woods Hole Oceanographic Institution"}}],"deployments":[{"_id":"956118","name":"EN683","synonyms":[],"dates":{"start":"2022-05-24T00:00:00.000Z","end":"2022-06-12T00:00:00.000Z"},"platform":{"_id":"53992","name":"R/V Endeavor"},"chiefScientists":[{"id":"661940","name":"Carol Arnosti","role":"Chief Scientist"}]}],"funding":[{"_id":"896821","name":"OCE-2022952","fundingSource":{"_id":"355","name":"NSF Division of Ocean Sciences"},"url":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=2022952"}],"instruments":[{"_id":"956130","name":"TECAN infiniteF200; 360 nm excitation, 460 emission","description":"","type_id":"528693","type_name":"plate reader","type_desc":"$1e","type_acronym":""}],"parameters":[{"_id":"956152","name":"Incubation","format":"","frictionlessType":"string","description":"

Mesocosm or Bulk Water Incubation

","units":{"_id":"","name":"unitless"},"type":{"_id":"1741","name":"sample_descrip","description":"

text description or other traits of the sample collected

"}},{"_id":"956153","name":"deployment","format":"","frictionlessType":"string","description":"

Cruise ID

","units":{"_id":"","name":"unitless"},"type":{"_id":"1929","name":"Cruise Name","description":"

The cruise_name is a local name chosen by project investigators for a research expedition on a vessel, as opposed to the formal/official cruise ID asserted by the vessel operator.

"}},{"_id":"956154","name":"Station","format":"","frictionlessType":"integer","description":"

Station number for cruise

","units":{"_id":"","name":"unitless"},"type":{"_id":"987","name":"station","description":"

Station identifier; a unique number or alphanumeric string designating a general geographic location at which one or more sampling activities may occur.

"}},{"_id":"956155","name":"longitude","format":"","frictionlessType":"number","description":"

Longitude, west is negative

","units":{"_id":"","name":"decimal degrees"},"type":{"_id":"731","name":"longitude","description":"

longitude, in decimal degrees, East is positive, negative denotes West; Reported in some datasets as degrees, minutes

"}},{"_id":"956156","name":"latitude","format":"","frictionlessType":"number","description":"

Latitude, south is negative

","units":{"_id":"","name":"decimal degrees"},"type":{"_id":"730","name":"latitude","description":"

latitude, in decimal degrees, North is positive, negative denotes South; Reported in some datasets as degrees, minutes

"}},{"_id":"956157","name":"date","format":"%Y-%m-%d","frictionlessType":"date","description":"

Date of sample collection in ISO format (yyyy-mm-dd), US Eastern Time (UTC-05:00)

","units":{"_id":"","name":"unitless"},"type":{"_id":"1070","name":"date","description":"

date; generally reported in GMT as YYYYMMDD (year; month; day); also as MMDD (month; day); EqPac dates are local Hawaii time. ISO_Date format is YYYY-MM-DD (http://www.iso.org/iso/home/standards/iso8601.htm)

"}},{"_id":"956158","name":"time","format":"%H:%M:%S","frictionlessType":"time","description":"

Time of sample collection in ISO format (hh:mm:ss), US Eastern Time (UTC-05:00)

","units":{"_id":"","name":"unitless"},"type":{"_id":"1075","name":"time","description":"

Time GMT defined as HHMM; time of day; generally reported in GMT as HHMM (hours and minutes) but may also be HH.H (decimal hours) or HHMMSS (hours; minutes and seconds); in EqPac time is reported as Hawaii local time

"}},{"_id":"956159","name":"ISO_DateTime_UTC","format":"%Y-%m-%dT%H:%M:%S","frictionlessType":"datetime","description":"

DateTime of sample collection in ISO format in GMT/UTC Time

","units":{"_id":"","name":"unitless"},"type":{"_id":"1908","name":"ISO_DateTime_UTC","description":"

Date/Time (UTC) ISO formatted
\nThis standard is based on ISO 8601:2004(E) and takes on any of the following forms:
\n2009-08-30T09:05:00[.xx] (local time)
\n2009-08-30T14:05:00[.xx]Z (UTC time)
\n2009-08-30T14:05:00[.xx]-05:00
\nThe dashes and the colons can be dropped.
\nThe T can also be dropped \"by mutual agreement\", but one needs the trailing Z if the time is UTC.

Sample ISO_datetime_utc:
\n2009-08-30T14:05:00[.xx]Z (UTC time)

Format: YYYY-mm-ddTHH:MM:SS[.xx]Z (UTC time)

"}},{"_id":"956160","name":"cast_number","format":"","frictionlessType":"integer","description":"

Cast number (refers to cast of CTD/Niskin bottles on cruise)

","units":{"_id":"","name":"unitless"},"type":{"_id":"761","name":"cast","description":"

cast or profile number

"}},{"_id":"956161","name":"depth_actual","format":"","frictionlessType":"number","description":"

Actual depth at which water was collected

","units":{"_id":"","name":"meters (m)"},"type":{"_id":"808","name":"depth","description":"

Observation/sample depth below the sea surface. Units often reported as: meters, feet.

\nWhen used in a JGOFS/GLOBEC dataset the depth is a best estimate; usually but not always calculated from pressure; calculated either from CTD pressure using Fofonoff and Millard (1982; UNESCO Tech Paper #44) algorithm adjusted for 1980 equation of state for seawater (EOS80) or simply equivalent to nominal depth as recorded during sampling if CTD pressure was unavailable.

"}},{"_id":"956162","name":"sample_type","format":"","frictionlessType":"string","description":"

Sample from bulk water or Large Volume incubation

","units":{"_id":"","name":"unitless"},"type":{"_id":"1742","name":"sample_type","description":"

text description of type of sample collected (rock, bio, fluid, etc)

"}},{"_id":"956163","name":"Incubation_Temp","format":"","frictionlessType":"string","description":"

Temperature of incubation, RT (~20-25°C).

","units":{"_id":"","name":"Degrees Celsius (°C)"},"type":{"_id":"733","name":"temperature","description":"

water temperature at measurement depth

"}},{"_id":"956164","name":"unamended_amended","format":"","frictionlessType":"string","description":"

Whether high molecular weight organic mater was added or not; U for unamended, F, A, T refer to type of organic mater added (Fucodian, Arabinogalactan, Thalassiosira extract), the following number corresponds to amended incubation replicate.

","units":{"_id":"","name":"unitless"},"type":{"_id":"1741","name":"sample_descrip","description":"

text description or other traits of the sample collected

"}},{"_id":"956165","name":"Sub_sample_day","format":"","frictionlessType":"integer","description":"

Days post amendment when subsample was taken prior to substate addition and enzymatic activity measurement.

","units":{"_id":"","name":"days"},"type":{"_id":"472028","name":"time_sample","description":"

Time when sample was taken. Typically used in laboratory experiments as time sample was collected. Could be hours, minutes, or even relative to a timeframe.

"}},{"_id":"956166","name":"substrate","format":"","frictionlessType":"string","description":"

Substrates for measurement of enzymatic activities:

* a-glu: substrate to measure alpha glucosidase: 4-methylumbelliferyl-α-D-
\n* b-glu: substrate to measure beta glucosidase: 4-methylumbelliferyl-β-D-
\n* L: substrate to measure leucine aminopeptidase (L-leucine-7-amido-4 MCA)
\n* AAF: substrate to measure chymotrypsin activity: ala-ala-phe-MCA
\n* AAPF: substrate to measure chymotrypsin activity: N-succinyl-ala-ala-pro-phe-MCA
\n* QAR: substrate to measure trypsin activity: Boc-gln-ala-arg-MCA
\n* FSR: substrate to measure trypsin activity: N-t-boc-phe-ser-arg-MCA

","units":{"_id":"","name":"unitless"},"type":{"_id":"1741","name":"sample_descrip","description":"

text description or other traits of the sample collected

"}},{"_id":"956167","name":"rate_6hr","format":"","frictionlessType":"number","description":"

Measured hydrolysis rate at indicated time interval (6hr, 12hr, 18hr, ...) post substrate addition. Blank = not measured

","units":{"_id":"","name":"nmol L-1 h-0"},"type":{"_id":"472028","name":"time_sample","description":"

Time when sample was taken. Typically used in laboratory experiments as time sample was collected. Could be hours, minutes, or even relative to a timeframe.

"}},{"_id":"956168","name":"sd_6hr","format":"","frictionlessType":"number","description":"

Standard deviation of measured hydrolysis rate at indicated time interval (6hr, 12hr, 18hr, ...) post substrate addition. Blank = not measured

","units":{"_id":"","name":"nmol L-1 h-1"},"type":{"_id":"472028","name":"time_sample","description":"

Time when sample was taken. Typically used in laboratory experiments as time sample was collected. Could be hours, minutes, or even relative to a timeframe.

"}},{"_id":"956169","name":"rate_12hr","format":"","frictionlessType":"number","description":"

Measured hydrolysis rate at indicated time interval (6hr, 12hr, 18hr, ...) post substrate addition. Blank = not measured

","units":{"_id":"","name":"nmol L-1 h-1"},"type":{"_id":"472028","name":"time_sample","description":"

Time when sample was taken. Typically used in laboratory experiments as time sample was collected. Could be hours, minutes, or even relative to a timeframe.

"}},{"_id":"956170","name":"sd_12hr","format":"","frictionlessType":"number","description":"

Standard deviation of measured hydrolysis rate at indicated time interval (6hr, 12hr, 18hr, ...) post substrate addition. Blank = not measured

","units":{"_id":"","name":"nmol L-1 h-1"},"type":{"_id":"472028","name":"time_sample","description":"

Time when sample was taken. Typically used in laboratory experiments as time sample was collected. Could be hours, minutes, or even relative to a timeframe.

"}},{"_id":"956171","name":"rate_18hr","format":"","frictionlessType":"number","description":"

Measured hydrolysis rate at indicated time interval (6hr, 12hr, 18hr, ...) post substrate addition. Blank = not measured

","units":{"_id":"","name":"nmol L-1 h-1"},"type":{"_id":"472028","name":"time_sample","description":"

Time when sample was taken. Typically used in laboratory experiments as time sample was collected. Could be hours, minutes, or even relative to a timeframe.

"}},{"_id":"956172","name":"sd_18hr","format":"","frictionlessType":"number","description":"

Standard deviation of measured hydrolysis rate at indicated time interval (6hr, 12hr, 18hr, ...) post substrate addition. Blank = not measured

","units":{"_id":"","name":"nmol L-1 h-1"},"type":{"_id":"472028","name":"time_sample","description":"

Time when sample was taken. Typically used in laboratory experiments as time sample was collected. Could be hours, minutes, or even relative to a timeframe.

"}},{"_id":"956173","name":"rate_24hr","format":"","frictionlessType":"number","description":"

Measured hydrolysis rate at indicated time interval (6hr, 12hr, 18hr, ...) post substrate addition. Blank = not measured

","units":{"_id":"","name":"nmol L-1 h-1"},"type":{"_id":"472028","name":"time_sample","description":"

Time when sample was taken. Typically used in laboratory experiments as time sample was collected. Could be hours, minutes, or even relative to a timeframe.

"}},{"_id":"956174","name":"sd_24hr","format":"","frictionlessType":"number","description":"

Standard deviation of measured hydrolysis rate at indicated time interval (6hr, 12hr, 18hr, ...) post substrate addition. Blank = not measured

","units":{"_id":"","name":"nmol L-1 h-1"},"type":{"_id":"472028","name":"time_sample","description":"

Time when sample was taken. Typically used in laboratory experiments as time sample was collected. Could be hours, minutes, or even relative to a timeframe.

"}},{"_id":"956175","name":"rate_36hr","format":"","frictionlessType":"number","description":"

Measured hydrolysis rate at indicated time interval (6hr, 12hr, 18hr, ...) post substrate addition. Blank = not measured

","units":{"_id":"","name":"nmol L-1 h-1"},"type":{"_id":"472028","name":"time_sample","description":"

Time when sample was taken. Typically used in laboratory experiments as time sample was collected. Could be hours, minutes, or even relative to a timeframe.

"}},{"_id":"956176","name":"sd_36hr","format":"","frictionlessType":"number","description":"

Standard deviation of measured hydrolysis rate at indicated time interval (6hr, 12hr, 18hr, ...) post substrate addition. Blank = not measured

","units":{"_id":"","name":"nmol L-1 h-1"},"type":{"_id":"472028","name":"time_sample","description":"

Time when sample was taken. Typically used in laboratory experiments as time sample was collected. Could be hours, minutes, or even relative to a timeframe.

"}},{"_id":"956177","name":"rate_48hr","format":"","frictionlessType":"number","description":"

Measured hydrolysis rate at indicated time interval (6hr, 12hr, 18hr, ...) post substrate addition. Blank = not measured

","units":{"_id":"","name":"nmol L-1 h-1"},"type":{"_id":"472028","name":"time_sample","description":"

Time when sample was taken. Typically used in laboratory experiments as time sample was collected. Could be hours, minutes, or even relative to a timeframe.

"}},{"_id":"956178","name":"sd_48hr","format":"","frictionlessType":"number","description":"

Standard deviation of measured hydrolysis rate at indicated time interval (6hr, 12hr, 18hr, ...) post substrate addition. Blank = not measured

","units":{"_id":"","name":"nmol L-1 h-1"},"type":{"_id":"472028","name":"time_sample","description":"

Time when sample was taken. Typically used in laboratory experiments as time sample was collected. Could be hours, minutes, or even relative to a timeframe.

"}},{"_id":"956179","name":"rate_72hr","format":"","frictionlessType":"number","description":"

Measured hydrolysis rate at indicated time interval (6hr, 12hr, 18hr, ...) post substrate addition. Blank = not measured

","units":{"_id":"","name":"nmol L-1 h-1"},"type":{"_id":"472028","name":"time_sample","description":"

Time when sample was taken. Typically used in laboratory experiments as time sample was collected. Could be hours, minutes, or even relative to a timeframe.

"}},{"_id":"956180","name":"sd_72hr","format":"","frictionlessType":"number","description":"

Standard deviation of measured hydrolysis rate at indicated time interval (6hr, 12hr, 18hr, ...) post substrate addition. Blank = not measured

","units":{"_id":"","name":"nmol L-1 h-1"},"type":{"_id":"472028","name":"time_sample","description":"

Time when sample was taken. Typically used in laboratory experiments as time sample was collected. Could be hours, minutes, or even relative to a timeframe.

"}}],"related_publications":[{"id":"825316","citation":"Hoppe, H.-G. (1983). Significance of exoenzymatic activities in the ecology of brackish water: measurements by means of methylumbelliferyl-substrates. Marine Ecology Progress Series, 11, 299–308. doi:10.3354/meps011299\r\n","relation_type":["Methods"],"relation_desc":""},{"id":"737001","citation":"Obayashi, Y., & Suzuki, S. (2005). Proteolytic enzymes in coastal surface seawater: Significant activity of endopeptidases and exopeptidases. Limnology and Oceanography, 50(2), 722–726. doi:10.4319/lo.2005.50.2.0722\r\n","relation_type":["Methods"],"relation_desc":""},{"id":"956131","citation":"ahoarfrost. (2025). ArnostiLab/ArnostiLab-RScript-Demo-PlateRdr: ArnostiLab-RScript-Demo-PlateRdr (Version v1.0.0) [Computer software]. Zenodo. https://doi.org/10.5281/ZENODO.14783119","relation_type":["Software"],"relation_desc":""}],"related_datasets":[{"_id":"985783","name":"EN683 Bacterial Productivity","url":"","relation_type":"IsRelatedTo","type_desc":"

The dataset is related to another dataset.

\n","relation_desc":"Includes data from mesocosm experiments performed using seawater samples collected on EN683.","citation":"Ghobrial, S., Arnosti, C. (2026) Bacterial Productivity measurement of bulk seawater and mesocosm experiments taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-10-02 doi:10.26008/1912/bco-dmo.985783.1"}],"services":[{"grouping":"","items":[{"name":"View Data","description":"An in-browser view of the primary tabular data file","url":"https://www.bco-dmo.org/dataset/956085/data/view","contentType":"text/html","actionType":"view"}]},{"grouping":"Metadata","items":[{"name":"HTML","description":"A simple HTML page of the dataset metadata","url":"https://www.bco-dmo.org/dataset/956085/description","contentType":"text/html","actionType":"view"},{"name":"PDF","description":"A PDF of the HTML dataset metadata","url":"https://www.bco-dmo.org/dataset/956085/Dataset_description.pdf","contentType":"application/pdf","actionType":"download"},{"name":"ISO 19115-2 (NOAA Profile)","description":"","url":"https://www.bco-dmo.org/dataset/956085/iso","contentType":"application/xml","actionType":"download"}]}],"programs":[],"projects":[{"_id":"896822","name":"Substrate structural complexity and abundance control distinct mechanisms of microbially-driven carbon cycling in the ocean","acronym":"Substrate complexity and microbes","description":"$1f"}],"archives":[{"id_value":"10.26008/1912/bco-dmo.956085.1","url":"https://doi.org/10.26008/1912/bco-dmo.956085.1","date_assigned":"2025-07-08","version_date":"2025-03-17","archiver":"Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)","archiver_id":"272"}],"license":{"name":"Creative Commons Attribution 4.0","id":"CC-BY-4.0","uri":"https://creativecommons.org/licenses/by/4.0/","spdxUri":"https://spdx.org/licenses/CC-BY-4.0"},"metrics":{"views":{"count":105},"downloads":{"count":170}}},"fileId":"967133"}]