http://lod.bco-dmo.org/id/dataset/956677
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-03-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Common garden experimental data on temperature effects in offspring of four Odontesthes regia populations collected along the Chilean coast from Sep 2023 to Dec 2025
2026-03-17
publication
2026-03-17
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-04-07
publication
https://doi.org/10.26008/1912/bco-dmo.956677.1
Hannes Baumann
University of Connecticut
principalInvestigator
Zofia A. Baumann
University of Connecticut
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Baumann, H., Baumann, Z. A. (2026) Common garden experimental data on temperature effects in offspring of four Odontesthes regia populations collected along the Chilean coast from Sep 2023 to Dec 2025. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2026-03-17 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.956677.2 [access date]
Odontesthes regia: 2023 and 2025 common garden experiment Dataset Description: Methods and Sampling: <p><strong>Field sampling of spawners, offspring production, transport&nbsp;</strong></p>
<p>At each of four locations along the Chilean coast (north to south: Iquique [IQ, 20<strong>°</strong>S], Caleta Sierra [CS, 31<strong>°</strong>S], Dichato [DI, 37<strong>°</strong>S], Puerto Montt [PM, 41<strong>°</strong>S]), flowing-ripe adults of the Chilean silverside (<em>Odontesthes regia</em>) (urn:lsid:marinespecies.org:taxname:281830)&nbsp;were obtained during the species’ spawning season in austral spring (September-October) in 2023 and 2025. With the help of local collaborators and/or local artisanal fishermen, adults were caught during night or early morning hours with gill nets of various lengths (2 cm stretched mesh). Fish were separated by sex and kept alive for up to one hour, before they were strip-spawned into plastic dishes filled with clean seawater. At least 20 spawners per sex and population were used if available. In 2023, the northernmost site of Iquique was sampled twice; we denoted this in the data as fertilization 1 and fertilization 2. Fertilized embryos were transported in cooler boxes or thermos containers to the rearing facility in Dichato via car (PM, DI, CS) or airplane (IQ). Upon arrival at the rearing facility no more than 24h post-fertilization, they were randomly distributed among temperature treatments and replicate rearing containers.</p>
<p><strong>Common garden experiment</strong></p>
<p>In both years, offspring were reared at the<strong> Dichato Marine Research Station;</strong> in 2023 the experimental setup was located at the <strong>INCAR</strong> facility (Centro Interdisciplinario para la Investigación Acuícola), whereas in 2025 the setup was housed at the Experimental Marine Biology Laboratory (aka ‘Acuario’) of the Universidad de Concepción. We used <strong>four 900-1200L recirculating tanks</strong> representing four temperature treatments (2023: 11, 14, 18, 23<strong>°</strong>C; 2025: 14, 17, 20, 23<strong>°</strong>C). Each tank housed up to 12 rearing containers that represented the 3 replicates for each of the 4 populations (<strong>4 temperatures × 4 populations × 3 replicates = 48 containers</strong>). The container design was identical to previous silverside experiments (e.g., Baumann &amp; Conover 2011, Murray et al. 2014), i.e., we used <strong>round, white 20L containers</strong> equipped with individual airlines and mesh-screened (150 µm) holes, which guaranteed ~100% oxygenation and water exchange with the surrounding tank while retaining food. Tank water of ~ 30 psu was drawn from Coliumo Bay, filtered/UV-sterilized, and controlled for temperature via thermostats connected to commercial aquarium heaters or chillers. Each tank was equipped with a HOBO® Pendant MX2201 temperature logger (Onset) that recorded conditions every 30 minutes in 2023 and every 15 minutes in 2025. For biofiltration we used 4 large FX4-250 (Fluval) canister filters. The photoperiod was 15h light: 9h dark throughout the entire experiment.</p>
<p>Starting at hatch, silverside larvae were fed <strong><em>ad libitum</em> (=excess) rations </strong>of newly hatched brine shrimp nauplii (<em>Artemia salina</em>, San Francisco strain, Brine Shrimp Direct) produced daily on site. <em>Ad libitum</em> rations were ensured by never allowing rearing containers to become nauplii depleted. 1-3 days post hatch, the number of fish in each rearing container was equalized to 130 <strong>to avoid density-dependent growth effects</strong>. Another standardization to n = 80 per container occurred when larvae reached approximately 15 mm total length (TL), and a final standardization to n = 40 occurred when larvae reach approximately 25 mm TL. When larvae reached approximately 35 mm TL, the rearing concluded and all survivors were sampled.</p>
<p><strong>Samples</strong></p>
<p>All collected adults – both spawners and non-spawners - were measured for TL and then preserved frozen (-20<strong>°</strong>C) for later vertebral number analysis. Experimental samples were first taken on the temperature- and population-specific day of peak hatch, when up to 20 hatchlings were preserved in 95% ethanol (0-hatch). The first and second samples were taken at an average TL of approximately 15 mm TL (1-15mm, larvae) and 25 mm TL (2-25mm, early juveniles) and preserved in 95% ethanol as well. All individuals from these intermediate samples were measured for TL using the open-source software ImageJ and digital pictures taken with an iPhone 11 in front of a gridded (7mm grid size) white background. The 95% ethanol was replaced 24h after initial fixation. The final sample was taken when individuals on average had reached approximately 35 mm TL (3-35mm). In 2023, survivors were measured for TL using calipers (nearest 0.1 mm) and blotted wet weight (nearest mg) and then preserved frozen (-20<strong>°</strong>C) in individual plastic bags. In 2025, final samples were only measured for TL via digital pictures in ImageJ 1.53a and preserved in 95% ethanol (same as intermediate samples).</p>
<p><strong>Vertebral number</strong></p>
<p>The number of vertebrae was determined for all adult fish, regardless of whether they were used to produce offspring or not. Fish were X-rayed at the Oceanside Animal Hospital in Sandwich, MA, using a Vet Ray veterinarian X-ray system (SEDECAL, model A6504-25) and settings that were slightly modified from those recommended for small exotic pets (lizards). Specifically, we used a voltage of 60 kVp, power of 320 mA, and a shutter speed of 16 milliseconds. Digital x-ray pictures were analyzed using the multipoint tool in ImageJ to mark each vertebra between but excluding the basioccipital and the urostyle. In 2025, additional populations from Chimbote and Ancón in Peru were x-rayed by collaborator Victor Aramaya from the Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas, Lima, Peru.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2313288 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2313288
completed
Hannes Baumann
University of Connecticut
860-405-9297
Department of Marine Sciences 1080 Shennecossett Road
Groton
CT
06340-6048
USA
hannes.baumann@uconn.edu
pointOfContact
Zofia A. Baumann
University of Connecticut
zofia.baumann@uconn.edu
pointOfContact
asNeeded
Dataset Version: 2
Unknown
Species
Year
Population
Longitude
Latitude
Fert_num
Tank
Ind
Temp_treat
Stage
Bucket_ID
Replicate
Fertilization_date
Hatch_date
Sample_date
dph
TL_mm
wW_mg
GR1_from_hatch
GR2_from_15MM
GR3_from_25MM
T1_fromH
T2_from15MM
T3_from25MM
calipers
iPhone 11
1200L recirculating tank
gill nets
HOBO® Pendant MX temperature logger
Mettler-Toledo
SEDECAL, model A6504-25
theme
None, User defined
species scientific name (binomial)
year
region
longitude
latitude
sample description
tank
individual
treatment
stage
replicate
date
time_elapsed
length
wet_weight_biomass
metabolic rate
mean
featureType
BCO-DMO Standard Parameters
calipers
Camera
circulating water bath
Gillnet
Onset HOBO Pendant MX2201 temperature logger
scale or balance
X-Ray Imaging
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Expanding the silverside system to quantify how climate gradients determine co- and countergradient adaptation strength in the ocean
https://befel.marinesciences.uconn.edu/expanding-the-silverside-system
Expanding the silverside system to quantify how climate gradients determine co- and countergradient adaptation strength in the ocean
<p><em>NSF Award Abstract:</em><br />
Many species have evolved adaptations to latitudinal climate gradients and studying these sheds light on how species will evolve in response to global climate change. To investigate adaptation in Chilean silversides, offspring of wild fish from four locations along the Chilean coast are being reared at four common temperatures. Differences in growth rates, vertebral number, and mercury uptake among populations from different latitudes indicate genetic differences due to local adaptation. The research team is integrating these Chilean silverside data with prior data from northern hemisphere silverside species to better understand the relationship between climate gradient strength and adaptation strength. This project provides training for two graduate students through a US-Chile graduate cross-cultural exchange. The research is being integrated into graduate and undergraduate courses taught in Chile and the research team is sharing the results with the public through a website and magazine articles.</p>
<p>This project advances the understanding of two forms of local adaptation, co-gradient variation (CoGV) and counter-gradient variation (CnGV), which underlie adaptation to large-scale, latitudinal climate gradients. Using a common garden experiment, the team is examining the relationship between temperature and genetic variation in Chilean silversides. Newly-fertilized offspring obtained from wild founders at four locations along the Chilean coast are being reared at four common temperatures to a common juvenile size. Differences among populations in trait measurements (growth capacity, vertebral number, and total mercury concentration) to test the hypotheses that 1) growth capacity increases with temperature and latitude (CnGV) and 2) vertebral number increases with latitude (CoGV). These data are being integrated with existing evidence from northern hemisphere silverside species to determine if there is a relationship between CnGV/CoGV strength and latitudinal gradient strength.</p>
Chilean silversides
largerWorkCitation
project
eng; USA
biota
oceans
-72.9375
-70.14638889
-41.50444444
-20.27333333
2023-09-23
2025-12-25
laboratory-based
0
BCO-DMO catalogue of parameters from Common garden experimental data on temperature effects in offspring of four Odontesthes regia populations collected along the Chilean coast from Sep 2023 to Dec 2025
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/995028.rdf
Name: Species
Units: unitless
Description: <p>All data in this table are for the Chilean silverside Odontesthes regia</p>
http://lod.bco-dmo.org/id/dataset-parameter/995029.rdf
Name: Year
Units: unitless
Description: <p>Year of the experiment (2023 or 2025)</p>
http://lod.bco-dmo.org/id/dataset-parameter/995030.rdf
Name: Population
Units: unitless
Description: <p>1 of 4 sampling locations = 'population' along the Chilean coast: Iquique, Caleta Sierra, Dichato, Puerto Montt</p>
http://lod.bco-dmo.org/id/dataset-parameter/995031.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of the sampling location, negative is West</p>
http://lod.bco-dmo.org/id/dataset-parameter/995033.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of the sampling location, negative is South</p>
http://lod.bco-dmo.org/id/dataset-parameter/995035.rdf
Name: Fert_num
Units: unitless
Description: <p>ID number of fertilization per population (1 or 2)</p>
http://lod.bco-dmo.org/id/dataset-parameter/995036.rdf
Name: Tank
Units: unitless
Description: <p>ID of the rearing tank (1,2,3, or 4)</p>
http://lod.bco-dmo.org/id/dataset-parameter/995037.rdf
Name: Ind
Units: unitless
Description: <p>Offspring ID per population, temperature, replicate, and sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/995038.rdf
Name: Temp_treat
Units: degrees Celsius
Description: <p>Target temperature treatment of the experiment: 11, 14, 17, 18, 20, or 23°C</p>
http://lod.bco-dmo.org/id/dataset-parameter/995039.rdf
Name: Stage
Units: unitless
Description: <p>Sample stage: 0 = hatch, 1 = 15 mm (larvae), 2 = 25 mm (juvenile), 3 = 35 mm (final juvenile sample)</p>
http://lod.bco-dmo.org/id/dataset-parameter/995040.rdf
Name: Bucket_ID
Units: unitless
Description: <p>Running ID of each rearing container</p>
http://lod.bco-dmo.org/id/dataset-parameter/995041.rdf
Name: Replicate
Units: unitless
Description: <p>ID of each replicate rearing container per population and temperature treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/995042.rdf
Name: Fertilization_date
Units: unitless
Description: <p>Date of offspring fertilization in the wild (adult Sampling date in supplemental tables adult_total_length" and "adult_vertebral_number")"</p>
http://lod.bco-dmo.org/id/dataset-parameter/995043.rdf
Name: Hatch_date
Units: unitless
Description: <p>Date of peak hatch of offspring</p>
http://lod.bco-dmo.org/id/dataset-parameter/995044.rdf
Name: Sample_date
Units: unitless
Description: <p>Date when offspring were sampled and measured for total length</p>
http://lod.bco-dmo.org/id/dataset-parameter/995045.rdf
Name: dph
Units: days
Description: <p>Days post hatch (dph) on the sampling date</p>
http://lod.bco-dmo.org/id/dataset-parameter/995046.rdf
Name: TL_mm
Units: Millimeter
Description: <p>Individual total length measured via digital, calibrated pictures</p>
http://lod.bco-dmo.org/id/dataset-parameter/995047.rdf
Name: wW_mg
Units: Milligram
Description: <p>Individual blotted wet weight (final samples in 2023 only)</p>
http://lod.bco-dmo.org/id/dataset-parameter/995048.rdf
Name: GR1_from_hatch
Units: Millimeter per day
Description: <p>Growth rate calculated as the change in total length from mean hatch divided by dph</p>
http://lod.bco-dmo.org/id/dataset-parameter/995049.rdf
Name: GR2_from_15MM
Units: Millimeter per day
Description: <p>Growth rate calculated as the change in total length from replicate mean total length at stage 1-15MM divided by days since 15MM</p>
http://lod.bco-dmo.org/id/dataset-parameter/995050.rdf
Name: GR3_from_25MM
Units: Millimeter per day
Description: <p>Growth rate calculated as the change in total length from replicate mean total length at stage 2-25MM divided by days since 25MM</p>
http://lod.bco-dmo.org/id/dataset-parameter/995051.rdf
Name: T1_fromH
Units: degrees Celsius
Description: <p>Mean actual temperature experienced since hatch</p>
http://lod.bco-dmo.org/id/dataset-parameter/995052.rdf
Name: T2_from15MM
Units: degrees Celsius
Description: <p>Mean actual temperature experienced since stage 1-15MM</p>
http://lod.bco-dmo.org/id/dataset-parameter/995053.rdf
Name: T3_from25MM
Units: degrees Celsius
Description: <p>Mean actual temperature experienced since stage 2-25MM</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
305540
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/cadcc411-f451-4628-91e4-20282e6cbf3b/content
download
https://doi.org/10.26008/1912/bco-dmo.956677.1
download
onLine
dataset
<p><strong>Field sampling of spawners, offspring production, transport&nbsp;</strong></p>
<p>At each of four locations along the Chilean coast (north to south: Iquique [IQ, 20<strong>°</strong>S], Caleta Sierra [CS, 31<strong>°</strong>S], Dichato [DI, 37<strong>°</strong>S], Puerto Montt [PM, 41<strong>°</strong>S]), flowing-ripe adults of the Chilean silverside (<em>Odontesthes regia</em>) (urn:lsid:marinespecies.org:taxname:281830)&nbsp;were obtained during the species’ spawning season in austral spring (September-October) in 2023 and 2025. With the help of local collaborators and/or local artisanal fishermen, adults were caught during night or early morning hours with gill nets of various lengths (2 cm stretched mesh). Fish were separated by sex and kept alive for up to one hour, before they were strip-spawned into plastic dishes filled with clean seawater. At least 20 spawners per sex and population were used if available. In 2023, the northernmost site of Iquique was sampled twice; we denoted this in the data as fertilization 1 and fertilization 2. Fertilized embryos were transported in cooler boxes or thermos containers to the rearing facility in Dichato via car (PM, DI, CS) or airplane (IQ). Upon arrival at the rearing facility no more than 24h post-fertilization, they were randomly distributed among temperature treatments and replicate rearing containers.</p>
<p><strong>Common garden experiment</strong></p>
<p>In both years, offspring were reared at the<strong> Dichato Marine Research Station;</strong> in 2023 the experimental setup was located at the <strong>INCAR</strong> facility (Centro Interdisciplinario para la Investigación Acuícola), whereas in 2025 the setup was housed at the Experimental Marine Biology Laboratory (aka ‘Acuario’) of the Universidad de Concepción. We used <strong>four 900-1200L recirculating tanks</strong> representing four temperature treatments (2023: 11, 14, 18, 23<strong>°</strong>C; 2025: 14, 17, 20, 23<strong>°</strong>C). Each tank housed up to 12 rearing containers that represented the 3 replicates for each of the 4 populations (<strong>4 temperatures × 4 populations × 3 replicates = 48 containers</strong>). The container design was identical to previous silverside experiments (e.g., Baumann &amp; Conover 2011, Murray et al. 2014), i.e., we used <strong>round, white 20L containers</strong> equipped with individual airlines and mesh-screened (150 µm) holes, which guaranteed ~100% oxygenation and water exchange with the surrounding tank while retaining food. Tank water of ~ 30 psu was drawn from Coliumo Bay, filtered/UV-sterilized, and controlled for temperature via thermostats connected to commercial aquarium heaters or chillers. Each tank was equipped with a HOBO® Pendant MX2201 temperature logger (Onset) that recorded conditions every 30 minutes in 2023 and every 15 minutes in 2025. For biofiltration we used 4 large FX4-250 (Fluval) canister filters. The photoperiod was 15h light: 9h dark throughout the entire experiment.</p>
<p>Starting at hatch, silverside larvae were fed <strong><em>ad libitum</em> (=excess) rations </strong>of newly hatched brine shrimp nauplii (<em>Artemia salina</em>, San Francisco strain, Brine Shrimp Direct) produced daily on site. <em>Ad libitum</em> rations were ensured by never allowing rearing containers to become nauplii depleted. 1-3 days post hatch, the number of fish in each rearing container was equalized to 130 <strong>to avoid density-dependent growth effects</strong>. Another standardization to n = 80 per container occurred when larvae reached approximately 15 mm total length (TL), and a final standardization to n = 40 occurred when larvae reach approximately 25 mm TL. When larvae reached approximately 35 mm TL, the rearing concluded and all survivors were sampled.</p>
<p><strong>Samples</strong></p>
<p>All collected adults – both spawners and non-spawners - were measured for TL and then preserved frozen (-20<strong>°</strong>C) for later vertebral number analysis. Experimental samples were first taken on the temperature- and population-specific day of peak hatch, when up to 20 hatchlings were preserved in 95% ethanol (0-hatch). The first and second samples were taken at an average TL of approximately 15 mm TL (1-15mm, larvae) and 25 mm TL (2-25mm, early juveniles) and preserved in 95% ethanol as well. All individuals from these intermediate samples were measured for TL using the open-source software ImageJ and digital pictures taken with an iPhone 11 in front of a gridded (7mm grid size) white background. The 95% ethanol was replaced 24h after initial fixation. The final sample was taken when individuals on average had reached approximately 35 mm TL (3-35mm). In 2023, survivors were measured for TL using calipers (nearest 0.1 mm) and blotted wet weight (nearest mg) and then preserved frozen (-20<strong>°</strong>C) in individual plastic bags. In 2025, final samples were only measured for TL via digital pictures in ImageJ 1.53a and preserved in 95% ethanol (same as intermediate samples).</p>
<p><strong>Vertebral number</strong></p>
<p>The number of vertebrae was determined for all adult fish, regardless of whether they were used to produce offspring or not. Fish were X-rayed at the Oceanside Animal Hospital in Sandwich, MA, using a Vet Ray veterinarian X-ray system (SEDECAL, model A6504-25) and settings that were slightly modified from those recommended for small exotic pets (lizards). Specifically, we used a voltage of 60 kVp, power of 320 mA, and a shutter speed of 16 milliseconds. Digital x-ray pictures were analyzed using the multipoint tool in ImageJ to mark each vertebra between but excluding the basioccipital and the urostyle. In 2025, additional populations from Chimbote and Ancón in Peru were x-rayed by collaborator Victor Aramaya from the Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas, Lima, Peru.</p>
Specified by the Principal Investigator(s)
<p><em>Outlier removal.</em> After data compilation, we first removed stunted or malformed fish via outlier analysis. For that, we computed the mean (TL<sub>mean</sub>) and standard deviation (TL<sub>SD</sub>) of TL for each population (IQ, CS, DI, PM), temperature (11, 14, 17, 18, 20, 23°C), replicate (1, 2, 3), and sample type (hatch, 1-15mm, 2-25mm, 3-35mm), then removed all specimens with a TL &lt; TL<sub>mean</sub> – 2 × TL<sub>SD </sub>from all further analyses.</p>
<p><em>Growth capacities.</em> We computed 3 different growth capacities, i.e., TL growth rates (mm per day) at excess feeding conditions; <em>GR1</em> refers to the growth rate of each sampled individual since hatch, while <em>GR2</em> describes growth since the 15mm subsample, and <em>GR3</em> describes growth since the 25 mm subsample. Therefore:</p>
<blockquote>
<p><em>GR1<sub>ijkl</sub> = (TL<sub>ijkl</sub> – TL_H<sub>kl</sub>) / dph<sub>kl</sub></em></p>
<p><em>GR2<sub>ijkl</sub> = (TL<sub>ijkl</sub> – TL_15<sub>jkl</sub>) / dp15<sub>kl</sub></em></p>
<p><em>GR3<sub>ijkl</sub> = (TL<sub>ijkl</sub> – TL_25<sub>jkl</sub>) / dp25<sub>kl</sub></em></p>
</blockquote>
<p>For the <em>i</em><sup>th</sup> individual of the <em>j</em><sup>th</sup> replicate of the <em>k</em><sup>th</sup> temperature of the <em>l</em><sup>th</sup> population, where TL_H is the mean TL at hatch, TL_15 is the mean TL at the 15mm subsample, and TL_25 is the mean TL at the 25mm subsample. The length differentials were then divided by the number of days post hatch (dph) or the number of days since the 15mm subsample (dp15) or the number of days since the 25mm subsample (dp25mm).</p>
<p><em>Actually experienced temperature conditions</em>. Logged temperatures were first averaged per day, month, year and then used to calculate actually experienced temperature means during each stage (i.e., hatch-15mm, 15-25mm, 25-35mm) for each population and temperature-treatment.</p>
Specified by the Principal Investigator(s)
**Version 1**
- Imported NSF_OCE 2313288 - BCO-DMO source data.xlsx, sheet indexes 5, 4, 3, 2, and 1, into the BCO-DMO system
- Converted all dates to YYYY-MM-DD ISO format
- Converted lat and lon to decimal degrees format
- Rounded lat and lon values to 4 decimal places
- Renamed fields to remove spaces and units from parameters to comply with BCO-DMO system and style requirements
- Removed Month, Day, and Year from sheet 4, to avoid duplication
- Exported files as 956677_v1_common_garden_exp.csv (main file), adult_total_length.csv (supplemental file), adult_vertebral_number.csv (supplemental file), and experimental_temperatures.csv (supplemental file)
Accepted species identifier confirmed on 2025-04-05.
**Version 2**
- Loaded sheet 5 from "NSF_OCE 2313288 - BCO-DMO source data 2023-25.xlsx"; missing values flagged as "" and "nd"
- Converted "Fertilization date", "Hatch date", and "Sample date" fields from "%d-%b-%y" format to ISO "%Y-%m-%d" date format
- Converted Longitude (forced West) and Latitude (forced South) from degrees-minutes-seconds to decimal degrees; rounded both to 4 decimal places
- Rounded "Longitude" and "Latitude" to 4 decimal places
- Renamed fields to remove spaces and special characters
- Loaded sheet 2 from same Excel file as resource "adult_total_length"; blank headers ignored; missing values flagged as "" and "nd"
- Converted "Sampling date" from "%m/%d/%y" format to ISO "%Y-%m-%d" date format
- Converted Longitude (forced West) and Latitude (forced South) from degrees-minutes-seconds to decimal degrees; rounded both to 4 decimal places
- Loaded sheet 3 from same Excel file as resource "adult_vertebral_number"; missing values flagged as "" and "nd"
- Converted Longitude (forced West) and Latitude (forced South) from degrees-minutes-seconds to decimal degrees; rounded both to 4 decimal places
- Converted "Sampling date" from "%m/%d/%y" format to ISO "%Y-%m-%d" date format (UTC)
- Loaded sheet 4 from same Excel file as resource "experimental_temperatures"; blank headers ignored; missing values flagged as "" and "nd"
- Converted "datetime" field from "%m/%d/%y %H:%M" format to ISO "%Y-%m-%dT%H:%M" datetime format in Chile/Continental timezone, output to new field "ISO_DateTime_Local"
- Deleted fields "Month", "Day", "Year", and "datetime" from "experimental_temperatures"
- Output resources saved as: 956677_v2_common_garden_exp.csv, adult_total_length.csv, adult_vertebral_number.csv, and experimental_temperatures.csv
Accepted species identifier confirmed on 2026-03-17.
**V2 Dataset changes**
-New 2025 values added to dataset
-The Methods section was updated to include the latest protocols
-Information about data gaps was added
-The Data Processing section was updated with information about outlier removal
Primary Datafile:
-The parameter PopLabel was previously used to denote multiple sampling events at the same site. This is now represented with the column "Fert_num" (Fert#)
-The parameter Temp is now "Temp_treat"; mainhatchday is now "Hatch_date"; sampleday is now "Sample_date"; ID is now "Ind"; Sample is now "Stage"
-The parameter BucketID is now Bucket_ID and is formatted with a B in front of the number
-Additional columns include Year, Tank, GR1_from_hatch, GR2_from_15MM, GR3_from_25MM, T1_fromH, T2_from15MM, T3_from25MM
adult_total_length.csv:
Total length is now represented in Millimeters
experimental_temperatures.csv:
Log ID was added
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
calipers
calipers
PI Supplied Instrument Name: calipers PI Supplied Instrument Description:In 2023, survivors were measured for TL using calipers (nearest 0.1 mm) and blotted wet weight (nearest mg) and then preserved frozen (-20°C) in individual plastic bags. In 2025, final samples were only measured for TL via digital pictures in ImageJ 1.53a and preserved in 95% ethanol (same as intermediate samples). Instrument Name: calipers Instrument Short Name: Instrument Description: A caliper (or "pair of calipers") is a device used to measure the distance between two opposite sides of an object. Many types of calipers permit reading out a measurement on a ruled scale, a dial, or a digital display.
iPhone 11
iPhone 11
PI Supplied Instrument Name: iPhone 11 PI Supplied Instrument Description:All individuals from these intermediate samples were measured for TL using the open-source software ImageJ and digital pictures taken with an iPhone 11 in front of a gridded (7mm grid size) white background. Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/
1200L recirculating tank
1200L recirculating tank
PI Supplied Instrument Name: 1200L recirculating tank PI Supplied Instrument Description:We used four 900-1200L recirculating tanks representing four temperature treatments (2023: 11, 14, 18, 23°C; 2025: 14, 17, 20, 23°C).
The container design was identical to previous silverside experiments (e.g., Baumann & Conover 2011, Murray et al. 2014), i.e., we used round, white 20L containers equipped with individual airlines and mesh-screened (150 µm) holes, which guaranteed ~100% oxygenation and water exchange with the surrounding tank while retaining food. Tank water of ~ 30 psu was drawn from Coliumo Bay, filtered/UV-sterilized, and controlled for temperature via thermostats connected to commercial aquarium heaters or chillers. Instrument Name: circulating water bath Instrument Short Name: Instrument Description: A device designed to regulate the temperature of a vessel by bathing it in water held at the desired temperature. [Definition Source: NCI]
gill nets
gill nets
PI Supplied Instrument Name: gill nets PI Supplied Instrument Description:With the help of local collaborators and/or local artisanal fishermen, adults were caught during night or early morning hours with gill nets of various lengths (2 cm stretched mesh). Instrument Name: Gillnet Instrument Short Name:Gillnet Instrument Description: Gillnetting uses curtains of netting that are suspended by a system of floats and weights; they can be anchored to the sea floor or allowed to float at the surface. A gillnet catches fish by their gills because the twine of the netting is very thin, and either the fish does not see the net or the net is set so that it traps the fish. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/65/
HOBO® Pendant MX temperature logger
HOBO® Pendant MX temperature logger
PI Supplied Instrument Name: HOBO® Pendant MX temperature logger PI Supplied Instrument Description:Each tank was equipped with a HOBO® Pendant MX2201 temperature logger (Onset) that recorded conditions every 30 minutes in 2023 and every 15 minutes in 2025. Instrument Name: Onset HOBO Pendant MX2201 temperature logger Instrument Short Name:Onset HOBO Pendant MX2201 Instrument Description: The Onset HOBO MX2201 is an in-situ instrument for wet or underwater applications. It supports soil temperature, temperature, and water temperature. A one-channel logger that records up to approximately 96,000 measurements or internal logger events with 8K bytes memory. It has a polypropylene housing case. Uses Bluetooth to transmit data. Can be used with a solar radiation shield. Measurement range: -20 deg C to 70 deg C. Accuracy: +/- 0.50 deg C from 0 deg C to 50 deg C. Water depth rating: 30.5 m
Mettler-Toledo
Mettler-Toledo
PI Supplied Instrument Name: Mettler-Toledo PI Supplied Instrument Description:In 2023, survivors were measured for TL using calipers (nearest 0.1 mm) and blotted wet weight (nearest mg) and then preserved frozen (-20°C) in individual plastic bags. In 2025, final samples were only measured for TL via digital pictures in ImageJ 1.53a and preserved in 95% ethanol (same as intermediate samples). Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/
SEDECAL, model A6504-25
SEDECAL, model A6504-25
PI Supplied Instrument Name: SEDECAL, model A6504-25 PI Supplied Instrument Description:Fish were X-rayed at the Oceanside Animal Hospital in Sandwich, MA, using a Vet Ray veterinarian X-ray system (SEDECAL, model A6504-25) and settings that were slightly modified from those recommended for small exotic pets (lizards). Instrument Name: X-Ray Imaging Instrument Short Name:X-Ray Instrument Description: A radiographic procedure using the emission of x-rays to form an image of the structure penetrated by the radiation.