http://lod.bco-dmo.org/id/dataset/956677
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-03-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Common garden experimental data on temperature effects in offspring of four Odontesthes regia populations collected along the Chilean coast from Sep to Oct 2023
2025-04-07
publication
2025-04-07
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-04-07
publication
https://doi.org/10.26008/1912/bco-dmo.956677.1
Hannes Baumann
University of Connecticut
principalInvestigator
Zofia A. Baumann
University of Connecticut
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Baumann, H., Baumann, Z. A. (2025) Common garden experimental data on temperature effects in offspring of four Odontesthes regia populations collected along the Chilean coast from Sep to Oct 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-04-07 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.956677.1 [access date]
Odontesthes regia: 2023 common garden experiment Dataset Description: Methods and Sampling: <p><strong>Field sampling of spawners, offspring production, transport&nbsp;</strong></p>
<p>At each of four locations along the Chilean coast (north to south: Iquique [IQ, 20<strong>°</strong>S], Caleta Sierra [CS, 31<strong>°</strong>S], Dichato [DI, 37<strong>°</strong>S], Puerto Montt [PM, 42<strong>°</strong>S]), flowing-ripe adults of the Chilean silverside (<em>Odontesthes regia</em>)&nbsp;(urn:lsid:marinespecies.org:taxname:281830) were obtained during the species’ spawning season in austral spring 2023 (September-October). With the help of local artisanal fishermen, adults were caught during night or early morning hours with gill nets of various lengths (2 cm stretched mesh). Fish were separated by sex and kept alive for up to one hour, before they were strip-spawned into plastic dishes filled with clean seawater. Our target was to use at least 20 spawners per sex and population, but the realized number of spawners varied. The northernmost site of Iquique was sampled twice; we denoted this in the data as IQ1 and IQ2. Fertilized embryos were transported in cooler boxes or thermos containers to the rearing facility in Dichato via car (PM, DI, CS) or airplane (IQ). Upon arrival at the rearing facility no more than 24h post-fertilization, they were randomly distributed among temperature treatments and replicate rearing containers.</p>
<p><strong>Common garden experiment</strong></p>
<p>Offspring were&nbsp;reared at the&nbsp;INCAR&nbsp;(Centro Interdisciplinario para la Investigación Acuícola) facility of the&nbsp;Dichato Marine Research Station&nbsp;of the&nbsp;Universidad de Concepción. We used&nbsp;four 1200L recirculating tanks&nbsp;representing the four temperature treatments (11, 14, 18, 23°C). Each tank housed 12 rearing containers that represented the 3 replicates for each of the 4 populations (4 temperatures × 4 populations × 3 replicates = 48 containers). The container design was identical to previous silverside experiments (e.g., Baumann &amp; Conover 2011, Murray et al. 2014), i.e., we used&nbsp;round, white 20L containers&nbsp;equipped with individual airlines and mesh-screened (150 µm) holes, which guaranteed ~100% oxygenation and water exchange with the surrounding tank while retaining food. Tank water of ~ 30 psu was drawn from Coliumo Bay, filtered/UV-sterilized, and controlled for temperature via thermostats connected to commercial aquarium heaters or chillers. Each tank was equipped with a HOBO® Pendant MX temperature logger (Onset) that recorded conditions every 30 minutes. For biofiltration we used 4 large FX4-250 (Fluval) canister filters. The photoperiod was 15h light: 9h dark throughout the entire experiment.</p>
<p>Starting at hatch, silverside larvae were fed&nbsp;<em>ad libitum</em>&nbsp;rations&nbsp;of newly hatched brine shrimp nauplii (<em>Artemia salina</em>, San Francisco strain, from Brine Shrimp Direct) produced daily on site.&nbsp;<em>Ad libitum</em>&nbsp;rations were ensured by never allowing rearing containers to become nauplii depleted. A few days after successful first feeding, the number of fish in each rearing container was equalized to 130&nbsp;avoid density-dependent growth effects. Another standardization to n = 80 per container occurred during when larvae reached approximately 15 mm total length (TL), and a final standardization to n = 40 occurred when larvae reach approximately 25 mm TL. When larvae reached approximately 35 mm TL, the rearing concluded and all survivors were sampled.</p>
<p><strong>Samples</strong></p>
<p>All collected adults – both spawners and non-spawners - were measured for TL and then preserved frozen (-20<strong>°</strong>C) for later vertebral number analysis. Experimental samples were first taken on the temperature- and population-specific day of hatch, when up to 200 hatchlings were preserved in 95% ethanol (0-hatch). The first and second larval samples were taken at approximately 15 mm TL (1-15mm) and 25 mm TL (2-25mm) and preserved in 95% ethanol as well. All larvae from these intermediate samples were measured for TL using the open-source software ImageJ (version 1.53a) and digital pictures taken with an I-Phone 11 in front of a gridded (5mm grid size) white background. The 95% ethanol was replaced once, at 24h after initial fixation. The final sample was taken at approximately 35 mm TL (3-35mm). All survivors were measured for TL using calipers (nearest 0.1 mm) and blotted wet weight (Mettler-Toledo; nearest mg) and then preserved frozen (-20<strong>°</strong>C) in individual plastic bags.</p>
<p><strong>Vertebral number</strong></p>
<p>The number of vertebrae was determined for all adult fish, regardless of whether they were used to produce offspring or not. Fish were X-rayed at the Oceanside Animal Hospital in Sandwich, MA, using a Vet Ray veterinarian X-ray system (SEDECAL, model A6504-25) and settings that were slightly modified from those recommended for small exotic pets (lizards). Specifically, we used a voltage of 60 kVp, power of 320 mA, and a shutter speed of 16 milliseconds. Digital x-ray pictures were analyzed using the multipoint tool in ImageJ to mark each vertebra between but excluding the basiocciptal and the urostyle.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2313288 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2313288
onGoing
Hannes Baumann
University of Connecticut
860-405-9297
Department of Marine Sciences 1080 Shennecossett Road
Groton
CT
06340-6048
USA
hannes.baumann@uconn.edu
pointOfContact
Zofia A. Baumann
University of Connecticut
zofia.baumann@uconn.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Species
Population
PopLabel
Longitude
Latitude
Temp
Replicate
BucketID
Fertilization_date
mainhatchday
sampleday
Sample
ID
TL
wW
calipers
iPhone 11
1200L recirculating tank
gill nets
HOBO® Pendant MX temperature logger
Mettler-Toledo
SEDECAL, model A6504-25
theme
None, User defined
species
region
longitude
latitude
temperature of sample or a generic measurement (not in-situ water temp)
replicate
tank
date
stage
sample identification
length
wet_weight_biomass
featureType
BCO-DMO Standard Parameters
calipers
Camera
circulating water bath
Gillnet
Onset HOBO Pendant MX2201 temperature logger
scale or balance
X-Ray Imaging
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Expanding the silverside system to quantify how climate gradients determine co- and countergradient adaptation strength in the ocean
https://befel.marinesciences.uconn.edu/expanding-the-silverside-system
Expanding the silverside system to quantify how climate gradients determine co- and countergradient adaptation strength in the ocean
<p><em>NSF Award Abstract:</em><br />
Many species have evolved adaptations to latitudinal climate gradients and studying these sheds light on how species will evolve in response to global climate change. To investigate adaptation in Chilean silversides, offspring of wild fish from four locations along the Chilean coast are being reared at four common temperatures. Differences in growth rates, vertebral number, and mercury uptake among populations from different latitudes indicate genetic differences due to local adaptation. The research team is integrating these Chilean silverside data with prior data from northern hemisphere silverside species to better understand the relationship between climate gradient strength and adaptation strength. This project provides training for two graduate students through a US-Chile graduate cross-cultural exchange. The research is being integrated into graduate and undergraduate courses taught in Chile and the research team is sharing the results with the public through a website and magazine articles.</p>
<p>This project advances the understanding of two forms of local adaptation, co-gradient variation (CoGV) and counter-gradient variation (CnGV), which underlie adaptation to large-scale, latitudinal climate gradients. Using a common garden experiment, the team is examining the relationship between temperature and genetic variation in Chilean silversides. Newly-fertilized offspring obtained from wild founders at four locations along the Chilean coast are being reared at four common temperatures to a common juvenile size. Differences among populations in trait measurements (growth capacity, vertebral number, and total mercury concentration) to test the hypotheses that 1) growth capacity increases with temperature and latitude (CnGV) and 2) vertebral number increases with latitude (CoGV). These data are being integrated with existing evidence from northern hemisphere silverside species to determine if there is a relationship between CnGV/CoGV strength and latitudinal gradient strength.</p>
Chilean silversides
largerWorkCitation
project
eng; USA
biota
oceans
-72.9375
-70.14638889
-41.50444444
-20.27333333
2023-09-23
2023-12-25
laboratory-based
0
BCO-DMO catalogue of parameters from Common garden experimental data on temperature effects in offspring of four Odontesthes regia populations collected along the Chilean coast from Sep to Oct 2023
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/957925.rdf
Name: Species
Units: unitless
Description: <p>All data in this table are for the Chilean silverside Odontesthes regia</p>
http://lod.bco-dmo.org/id/dataset-parameter/957926.rdf
Name: Population
Units: unitless
Description: <p>Sampling location or 'population' along the Chilean coast: Iquique, Caleta Sierra, Dichato, Puerto Montt</p>
http://lod.bco-dmo.org/id/dataset-parameter/957927.rdf
Name: PopLabel
Units: unitless
Description: <p>Abbreviated population label (IQ1 and IQ2 are used to describe two sampling events at the same location, see methods)</p>
http://lod.bco-dmo.org/id/dataset-parameter/957928.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of the sampling location, negative is West</p>
http://lod.bco-dmo.org/id/dataset-parameter/957929.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of the sampling location, negative is South</p>
http://lod.bco-dmo.org/id/dataset-parameter/957930.rdf
Name: Temp
Units: degrees Celsius
Description: <p>Target temperature treatment of the experiment: 11, 14, 18, or 23C</p>
http://lod.bco-dmo.org/id/dataset-parameter/957931.rdf
Name: Replicate
Units: unitless
Description: <p>ID of each replicate rearing container per population and temperature treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/957932.rdf
Name: BucketID
Units: unitless
Description: <p>Running ID of each rearing container</p>
http://lod.bco-dmo.org/id/dataset-parameter/957933.rdf
Name: Fertilization_date
Units: unitless
Description: <p>Date of offspring fertilization in the wild (adult Sampling date in supplemental tables "adult_total_length" and "adult_vertebral_number")</p>
http://lod.bco-dmo.org/id/dataset-parameter/957934.rdf
Name: mainhatchday
Units: unitless
Description: <p>Date when most offspring hatched</p>
http://lod.bco-dmo.org/id/dataset-parameter/957935.rdf
Name: sampleday
Units: unitless
Description: <p>Date when offspring where sampled and measured for total length</p>
http://lod.bco-dmo.org/id/dataset-parameter/957936.rdf
Name: Sample
Units: unitless
Description: <p>Sample stage: 0 - hatch, 1 -larvae 15 mm, 2 - juvenile 25 mm, 3 - final sample 35 mm</p>
http://lod.bco-dmo.org/id/dataset-parameter/957937.rdf
Name: ID
Units: unitless
Description: <p>Offspring ID per population, temperature, replicate, and sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/957938.rdf
Name: TL
Units: millimeter (mm)
Description: <p>Individual total length measured via digital, calibrated pictures</p>
http://lod.bco-dmo.org/id/dataset-parameter/957939.rdf
Name: wW
Units: milligram (mg)
Description: <p>Individual blotted wet weight (final samples only)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
305540
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/cadcc411-f451-4628-91e4-20282e6cbf3b/content
download
https://doi.org/10.26008/1912/bco-dmo.956677.1
download
onLine
dataset
<p><strong>Field sampling of spawners, offspring production, transport&nbsp;</strong></p>
<p>At each of four locations along the Chilean coast (north to south: Iquique [IQ, 20<strong>°</strong>S], Caleta Sierra [CS, 31<strong>°</strong>S], Dichato [DI, 37<strong>°</strong>S], Puerto Montt [PM, 42<strong>°</strong>S]), flowing-ripe adults of the Chilean silverside (<em>Odontesthes regia</em>)&nbsp;(urn:lsid:marinespecies.org:taxname:281830) were obtained during the species’ spawning season in austral spring 2023 (September-October). With the help of local artisanal fishermen, adults were caught during night or early morning hours with gill nets of various lengths (2 cm stretched mesh). Fish were separated by sex and kept alive for up to one hour, before they were strip-spawned into plastic dishes filled with clean seawater. Our target was to use at least 20 spawners per sex and population, but the realized number of spawners varied. The northernmost site of Iquique was sampled twice; we denoted this in the data as IQ1 and IQ2. Fertilized embryos were transported in cooler boxes or thermos containers to the rearing facility in Dichato via car (PM, DI, CS) or airplane (IQ). Upon arrival at the rearing facility no more than 24h post-fertilization, they were randomly distributed among temperature treatments and replicate rearing containers.</p>
<p><strong>Common garden experiment</strong></p>
<p>Offspring were&nbsp;reared at the&nbsp;INCAR&nbsp;(Centro Interdisciplinario para la Investigación Acuícola) facility of the&nbsp;Dichato Marine Research Station&nbsp;of the&nbsp;Universidad de Concepción. We used&nbsp;four 1200L recirculating tanks&nbsp;representing the four temperature treatments (11, 14, 18, 23°C). Each tank housed 12 rearing containers that represented the 3 replicates for each of the 4 populations (4 temperatures × 4 populations × 3 replicates = 48 containers). The container design was identical to previous silverside experiments (e.g., Baumann &amp; Conover 2011, Murray et al. 2014), i.e., we used&nbsp;round, white 20L containers&nbsp;equipped with individual airlines and mesh-screened (150 µm) holes, which guaranteed ~100% oxygenation and water exchange with the surrounding tank while retaining food. Tank water of ~ 30 psu was drawn from Coliumo Bay, filtered/UV-sterilized, and controlled for temperature via thermostats connected to commercial aquarium heaters or chillers. Each tank was equipped with a HOBO® Pendant MX temperature logger (Onset) that recorded conditions every 30 minutes. For biofiltration we used 4 large FX4-250 (Fluval) canister filters. The photoperiod was 15h light: 9h dark throughout the entire experiment.</p>
<p>Starting at hatch, silverside larvae were fed&nbsp;<em>ad libitum</em>&nbsp;rations&nbsp;of newly hatched brine shrimp nauplii (<em>Artemia salina</em>, San Francisco strain, from Brine Shrimp Direct) produced daily on site.&nbsp;<em>Ad libitum</em>&nbsp;rations were ensured by never allowing rearing containers to become nauplii depleted. A few days after successful first feeding, the number of fish in each rearing container was equalized to 130&nbsp;avoid density-dependent growth effects. Another standardization to n = 80 per container occurred during when larvae reached approximately 15 mm total length (TL), and a final standardization to n = 40 occurred when larvae reach approximately 25 mm TL. When larvae reached approximately 35 mm TL, the rearing concluded and all survivors were sampled.</p>
<p><strong>Samples</strong></p>
<p>All collected adults – both spawners and non-spawners - were measured for TL and then preserved frozen (-20<strong>°</strong>C) for later vertebral number analysis. Experimental samples were first taken on the temperature- and population-specific day of hatch, when up to 200 hatchlings were preserved in 95% ethanol (0-hatch). The first and second larval samples were taken at approximately 15 mm TL (1-15mm) and 25 mm TL (2-25mm) and preserved in 95% ethanol as well. All larvae from these intermediate samples were measured for TL using the open-source software ImageJ (version 1.53a) and digital pictures taken with an I-Phone 11 in front of a gridded (5mm grid size) white background. The 95% ethanol was replaced once, at 24h after initial fixation. The final sample was taken at approximately 35 mm TL (3-35mm). All survivors were measured for TL using calipers (nearest 0.1 mm) and blotted wet weight (Mettler-Toledo; nearest mg) and then preserved frozen (-20<strong>°</strong>C) in individual plastic bags.</p>
<p><strong>Vertebral number</strong></p>
<p>The number of vertebrae was determined for all adult fish, regardless of whether they were used to produce offspring or not. Fish were X-rayed at the Oceanside Animal Hospital in Sandwich, MA, using a Vet Ray veterinarian X-ray system (SEDECAL, model A6504-25) and settings that were slightly modified from those recommended for small exotic pets (lizards). Specifically, we used a voltage of 60 kVp, power of 320 mA, and a shutter speed of 16 milliseconds. Digital x-ray pictures were analyzed using the multipoint tool in ImageJ to mark each vertebra between but excluding the basiocciptal and the urostyle.</p>
Specified by the Principal Investigator(s)
<p>We removed stunted or malformed fish from the dataset via low-outlier analysis. For each population (IQ, CS, DI, PM), temperature (11,14,18,23°C), replicate (1, 2, 3), and sample type (hatch, 1-15mm, 2-25mm, 3-35mm), we calculated the mean and standard deviation, then removed all specimens that were smaller than the mean – 2 × SD (outlier &lt; mean TL – 2*SD TL).</p>
Specified by the Principal Investigator(s)
- Imported NSF_OCE 2313288 - BCO-DMO source data.xlsx, sheet indexes 5, 4, 3, 2, and 1, into the BCO-DMO system
- Converted all dates to YYYY-MM-DD ISO format
- Converted lat and lon to decimal degrees format
- Rounded lat and lon values to 4 decimal places
- Renamed fields to remove spaces and units from parameters to comply with BCO-DMO system and style requirements
- Removed Month, Day, and Year from sheet 4, to avoid duplication
- Exported files as 956677_v1_common_garden_exp.csv (main file), adult_total_length.csv (supplemental file), adult_vertebral_number.csv (supplemental file), and experimental_temperatures.csv (supplemental file)
Accepted species identifier confirmed on 2025-04-05.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
calipers
calipers
PI Supplied Instrument Name: calipers PI Supplied Instrument Description:All survivors were measured for TL using calipers (nearest 0.1 mm) and blotted wet weight (Mettler-Toledo; nearest mg) and then preserved frozen (-20°C) in individual plastic bags. Instrument Name: calipers Instrument Short Name: Instrument Description: A caliper (or "pair of calipers") is a device used to measure the distance between two opposite sides of an object. Many types of calipers permit reading out a measurement on a ruled scale, a dial, or a digital display.
iPhone 11
iPhone 11
PI Supplied Instrument Name: iPhone 11 PI Supplied Instrument Description:All larvae from these intermediate samples were measured for TL using the open-source software ImageJ and digital pictures taken with an I-Phone 11 in front of a gridded (5mm grid size) white background. Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/
1200L recirculating tank
1200L recirculating tank
PI Supplied Instrument Name: 1200L recirculating tank PI Supplied Instrument Description:We used four 1200L recirculating tanks representing the four temperature treatments (11, 14, 18, 23°C). Each tank housed 12 rearing containers that represented the 3 replicates for each of the 4 populations (4 temperatures × 4 populations × 3 replicates = 48 containers).
The container design was identical to previous silverside experiments (e.g., Baumann & Conover 2011, Murray et al. 2014), i.e., we used round, white 20L containers equipped with individual airlines and mesh-screened (150 µm) holes, which guaranteed ~100% oxygenation and water exchange with the surrounding tank while retaining food. Tank water of ~ 30 psu was drawn from Coliumo Bay, filtered/UV-sterilized, and controlled for temperature via thermostats connected to commercial aquarium heaters or chillers. Instrument Name: circulating water bath Instrument Short Name: Instrument Description: A device designed to regulate the temperature of a vessel by bathing it in water held at the desired temperature. [Definition Source: NCI]
gill nets
gill nets
PI Supplied Instrument Name: gill nets PI Supplied Instrument Description:With the help of local artisanal fishermen, adults were caught during night or early morning hours with gill nets of various lengths (2 cm stretched mesh). Instrument Name: Gillnet Instrument Short Name:Gillnet Instrument Description: Gillnetting uses curtains of netting that are suspended by a system of floats and weights; they can be anchored to the sea floor or allowed to float at the surface. A gillnet catches fish by their gills because the twine of the netting is very thin, and either the fish does not see the net or the net is set so that it traps the fish. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/65/
HOBO® Pendant MX temperature logger
HOBO® Pendant MX temperature logger
PI Supplied Instrument Name: HOBO® Pendant MX temperature logger PI Supplied Instrument Description:Each tank was equipped with a HOBO® Pendant MX temperature logger (Onset) that recorded conditions every 30 minutes. Instrument Name: Onset HOBO Pendant MX2201 temperature logger Instrument Short Name:Onset HOBO Pendant MX2201 Instrument Description: The Onset HOBO MX2201 is an in-situ instrument for wet or underwater applications. It supports soil temperature, temperature, and water temperature. A one-channel logger that records up to approximately 96,000 measurements or internal logger events with 8K bytes memory. It has a polypropylene housing case. Uses Bluetooth to transmit data. Can be used with a solar radiation shield. Measurement range: -20 deg C to 70 deg C. Accuracy: +/- 0.50 deg C from 0 deg C to 50 deg C. Water depth rating: 30.5 m
Mettler-Toledo
Mettler-Toledo
PI Supplied Instrument Name: Mettler-Toledo PI Supplied Instrument Description:All survivors were measured for blotted wet weight (nearest mg) using a Mettler-Toledo scale. Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/
SEDECAL, model A6504-25
SEDECAL, model A6504-25
PI Supplied Instrument Name: SEDECAL, model A6504-25 PI Supplied Instrument Description:Fish were X-rayed at the Oceanside Animal Hospital in Sandwich, MA, using a Vet Ray veterinarian X-ray system (SEDECAL, model A6504-25) and settings that were slightly modified from those recommended for small exotic pets (lizards). Instrument Name: X-Ray Imaging Instrument Short Name:X-Ray Instrument Description: A radiographic procedure using the emission of x-rays to form an image of the structure penetrated by the radiation.