http://lod.bco-dmo.org/id/dataset/958726
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-04-10
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Larval fish counts and larval tuna counts with corresponding plankton net type and geographic coordinates from R/V Roger Revelle cruise RR2201 (BLOOFINZ-IO, January-March 2022) in the Argo Basin region off NW Australia
2025-05-05
publication
2025-05-05
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-05-06
publication
https://doi.org/10.26008/1912/bco-dmo.958726.1
Estrella Malca
University of Miami
principalInvestigator
David J. Die
University of Miami
principalInvestigator
Raul Laiz Carrion
Spanish Institute of Oceanography
principalInvestigator
Jose Maria Quintanilla
Spanish National Research Council
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Malca, E., Die, D. J., Laiz Carrion, R., Jivanjee, A. A., Feltz, A., Carr, M., Lashley, C. L., Quintanilla, J. M. (2025) Larval fish counts and larval tuna counts with corresponding plankton net type and geographic coordinates from R/V Roger Revelle cruise RR2201 (BLOOFINZ-IO, January-March 2022) in the Argo Basin region off NW Australia. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-05-05 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.958726.1 [access date]
BLOOFINZ-IO larval tuna counts and abundances Dataset Description: Methods and Sampling: <p>Larval tuna sampling was done by three net systems fished in the upper 25-30 meters (m) of the water column. The main gear utilized was the Bongo90. Standard larval sampling was done with 10-minute (~2 knots) oblique hauls in the upper 25 m of the water column using a dual 90-centimeter (cm) diameter bongo frame with 505-micrometer (μm) mesh nets (Laiz-Carrion et al., 2015). Filtered volumes were calculated with mechanical flowmeters (2030R, General Oceanics Inc.) centered in each net mouth. Bongo90 tows were generally done with a smaller "mini-bongo" net (20-cm diameter mouth; 200 and 50-μm mesh nets with flowmeters) attached above the larger nets to sample zooplankton prey (Shiroza et al., 2021). A second net, the 'Black Widow' was the name given to a modified Bongo90 frame with black-dyed nets (1000 µm mesh), was towed at higher speed at night with a depressor (and without the mini-bongo) to collect larger, more evasive larvae. The Black Widow net was lost due to a break in the Kevlar line on tow 54 (Cycle 2, 9 February).The third net utilized was the neuston net. The neuston was a square single-frame coarse-mesh net (1 square meter, with 1000-µm mesh) that was towed at the sea surface. Although operated at lower speed (≤2 kts) than the Black Widow, it was also successful in collecting larger larvae after the widow was lost.</p>
<p>Freshly collected samples (one side of the bongo net collections or the Neuston) were put on ice and live sorted and sized to species and developmental stage (preflexion, flexion, postflexion) at-sea following Richards et al., 2005. Sorted specimens were numbered and either frozen or EtOH preserved in individual vials for later analyses of gut contents (feeding), otolith microstructure (growth), and isotopic composition (trophic position). The second sides of bongo net collections were preserved whole by a multi-step process that replaced residual water with (ethyl ethanol 95%, EtOH) after 24 hours. Shipboard sorting yielded over 3700 larval tuna specimens, dominated (~86%) by southern bluefin tuna (SBT,<em> Thunnus maccoyii)</em> which were most abundant along the southern margin of the Argo Basin compared to the central basin. Yellowfin tuna (YFT, <em>T. albacares</em>) was second in relative abundance (~7%), and the remainder were relatively equally divided among T. albacore tuna (ALB, T<em>. alalunga</em> ), big eye tuna (BET, <em>T. obesus),</em> and skipjack tuna (SKJ, <em>Katsuwonus pelamis)</em>. A mass of freshly spawned eggs sampled on one occasion and subsequently confirmed to be SBT by genetic analysis will be important in isotopic interpretation of larval trophic position by providing a correction for the maternal contribution.</p>
<p>Laboratory analysis at the University of Miami was conducted by first measuring plankton displacement volume and wet weight of plankton samples. Next, the preservative (EtOH) was buffered by adding 125 milliliters (mL) of Tris buffer to a 5-gallon carboy. All plankton jars were transferred into buffered EtOH, and subsequent vials contained buffered EtOH. Next, a thorough examination of the right side (R) Bongo90 plankton samples was conducted under 0.63 - 4x magnification using a stereomicroscope to make sure that shipboard sorting did not miss any scombrid larvae. All larval fish were removed from a subset (n=67) of tows and from a 1/4 aliquot split (n=21) of Bongo90 samples. Scombrid larvae were identified to the lowest taxonomic level possible, and were labelled as '<em>Thunnus Sp.'</em> when species-level identification was not possible. Scombrid larvae were removed from an additional 38 sets of tows without removing other taxa to expedite sample processing of scombrid larvae for collaborative projects.</p>
<p>Over 2400 <em>Thunnus</em> larvae were individually examined, assigned a developmental stage, and given a unique identification number; a subset (n=480) was measured and photographed. Larval scombrids were shipped to collaborators for stomach content identification, prey preference analysis, otolith removal for ageing, genetics (PCR multiplex-identification, genome mapping), isotope analysis, and compound-specific isotope analysis.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1851395 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1851395
completed
Estrella Malca
University of Miami
Miami
FL
USA
estrella.malcaphd@gmail.com
pointOfContact
David J. Die
University of Miami
FL
USA
David.Die@noaa.gov
pointOfContact
Raul Laiz Carrion
Spanish Institute of Oceanography
Spain
raul.laiz@ieo.csic.es
pointOfContact
Jose Maria Quintanilla
Spanish National Research Council
jose.quintanilla@ieo.csic.es
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Event_No
ISO_DateTime_UTC
Date
Time
Latitude
Longitude
Tow
Net_Type
Mesh
Side
Tow_Depth
Tow_Vol
Sample_ID
Cycle
Sample_Status
SBT_Count
ALB_Count
YFT_Count
BET_Count
Thun_Count
SKJ_Count
Tuna_Count
Fish_Count
SBT_1000m3
ALB_1000m3
YFT_1000m3
BET_1000m3
Thun_1000m3
SKJ_1000m3
Tuna_1000m3
Fish_1000m3
Bongo90
mechanical flowmeters (2030R, General Oceanics Inc.)
neuston net
Sensus Ultra U-15763, Reefnet Inc.
theme
None, User defined
event
ISO_DateTime_UTC
date
time of day
latitude
longitude
tow
net
net mesh size
sample description
depth
volume
sample identification
count
abundance
featureType
BCO-DMO Standard Parameters
Bongo Net
Flow Meter
Neuston Net
Reefnet Sensus Ultra
instrument
BCO-DMO Standard Instruments
RR2201
service
Deployment Activity
Eastern Indian Ocean (Argo Basin)
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Second International Indian Ocean Expedition
https://web.whoi.edu/iioe2/
Second International Indian Ocean Expedition
Description from the program website:
The Second International Indian Ocean Expedition (IIOE-2) is a major global scientific program which will engage the international scientific community in collaborative oceanographic and atmospheric research from coastal environments to the deep sea over the period 2015-2020, revealing new information on the Indian Ocean (i.e. its currents, its influence upon the climate, its marine ecosystems) which is fundamental for future sustainable development and expansion of the Indian Ocean's blue economy. A large number of scientists from research institutions from around the Indian Ocean and beyond are planning their involvement in IIOE-2 in accordance with the overarching six scientific themes of the program. Already some large collaborative research projects are under development, and it is anticipated that by the time these projects are underway, many more will be in planning or about to commence as the scope and global engagement in IIOE-2 grows.
Focused research on the Indian Ocean has a number of benefits for all nations. The Indian Ocean is complex and drives the region's climate including extreme events (e.g. cyclones, droughts, severe rains, waves and storm surges). It is the source of important socio-economic resources (e.g. fisheries, oil and gas exploration/extraction, eco-tourism, and food and energy security) and is the background and focus of many of the region's human populations around its margins. Research and observations supported through IIOE-2 will result in an improved understanding of the ocean's physical and biological oceanography, and related air-ocean climate interactions (both in the short-term and long-term). The IIOE-2's program will complement and harmonise with other regional programs underway and collectively the outcomes of IIOE-2 will be of huge benefit to individual and regional sustainable development as the information is a critical component of improved decision making in areas such as maritime services and safety, environmental management, climate monitoring and prediction, food and energy security.
IIOE-2 activities will also include a significant focus on building the capacity of all nations around the Indian Ocean to understand and apply observational data or research outputs for their own socio-economic requirements and decisions. IIOE-2 capacity building programs will therefore be focused on the translation of the science and information outputs for societal benefit and training of relevant individuals from surrounding nations in these areas.
A Steering Committee was established to support U.S. participation in IIOE-2. More information is available on their website at https://web.whoi.edu/iioe2/.
IIOE-2
largerWorkCitation
program
Collaborative Research: Mesoscale variability in nitrogen sources and food-web dynamics supporting larval southern bluefin tuna in the eastern Indian Ocean
https://osprey.bco-dmo.org/project/819488
Collaborative Research: Mesoscale variability in nitrogen sources and food-web dynamics supporting larval southern bluefin tuna in the eastern Indian Ocean
<p><em>NSF Award Abstract:</em><br />
The small area between NW Australia and Indonesia in the eastern Indian Ocean (IO) is the only known spawning ground of Southern Bluefin Tuna (SBT), a critically endangered top marine predator. Adult SBT migrate thousands of miles each year from high latitude feeding areas to lay their eggs in these tropical waters, where food concentrations on average are below levels that can support optimal feeding and growth of their larvae. Many critical aspects of this habitat are poorly known, such as the main source of nitrogen nutrient that sustains system productivity, how the planktonic food web operates to produce the unusual types of zooplankton prey that tuna larvae prefer, and how environmental differences in habitat quality associated with ocean fronts and eddies might be utilized by adult spawning tuna to give their larvae a greater chance for rapid growth and survival success. This project investigates these questions on a 38-day expedition in early 2021, during the peak time of SBT spawning. This project is a US contribution to the 2nd International Indian Ocean Expedition (IIOE-2) that advances understanding of biogeochemical and ecological dynamics in the poorly studied eastern IO. This is the first detailed study of nitrogen and carbon cycling in the region linking Pacific and IO waters. The shared dietary preferences of SBT larvae with those of other large tuna and billfish species may also make the insights gained broadly applicable to understanding larval recruitment issues for top consumers in other marine ecosystems. New information from the study will enhance international management efforts for SBT. The shared larval dietary preferences of large tuna and billfish species may also extend the insights gained broadly to many other marine top consumers, including Atlantic bluefin tuna that spawn in US waters of the Gulf of Mexico. The end-to-end study approach, highlights connections among physical environmental variability, biogeochemistry, and plankton food webs leading to charismatic and economically valuable fish production, is the theme for developing educational tools and modules through the "scientists-in-the-schools" program of the Center for Ocean-Atmospheric Prediction Studies at Florida State University, through a program for enhancing STEM learning pathways for underrepresented students in Hawaii, and through public outreach products for display at the Birch Aquarium in San Diego. The study also aims to support an immersive field experience to introduce talented high school students to marine research, with the goal of developing a sustainable marine-related educational program for underrepresented students in rural northwestern Florida.</p>
<p>Southern Bluefin Tuna (SBT) migrate long distances from high-latitude feeding grounds to spawn exclusively in a small oligotrophic area of the tropical eastern Indian Ocean (IO) that is rich in mesoscale structures, driven by complex currents and seasonally reversing monsoonal winds. To survive, SBT larvae must feed and grow rapidly under environmental conditions that challenge conventional understanding of food-web structure and functional relationships in poor open-ocean systems. The preferred prey of SBT larvae, cladocerans and Corycaeidae copepods, are poorly studied and have widely different implications for trophic transfer efficiencies to larvae. Differences in nitrogen sources - N fixation vs deep nitrate of Pacific origin - to sustain new production in the region also has implications for conditions that may select for prey types (notably cladocerans) that enhance transfer efficiency and growth rates of SBT larvae. The relative importance of these N sources for the IO ecosystem may affect SBT resiliency to projected increased ocean stratification. This research expedition investigates how mesoscale variability in new production, food-web structure and trophic fluxes affects feeding and growth conditions for SBT larvae. Sampling across mesoscale features tests hypothesized relationships linking variability in SBT larval feeding and prey preferences (gut contents), growth rates (otolith analyses) and trophic positions (TP) to the environmental conditions of waters selected by adult spawners. Trophic Positions of larvae and their prey are determined using Compound-Specific Isotope Analyses of Amino Acids (CSIA-AA). Lagrangian experiments investigate underlying process rates and relationships through measurements of water-column 14C productivity, N2 fixation, 15NO3- uptake and nitrification; community biomass and composition (flow cytometry, pigments, microscopy, in situ imaging, genetic analyses); and trophic fluxes through micro- and mesozooplankton grazing, remineralization and export. Biogeochemical and food web elements of the study are linked by CSIA-AA (N source, TP), 15N-constrained budgets and modeling. The project elements comprise an end-to-end coupled biogeochemistry-trophic study as has not been done previously for any pelagic ecosystem.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
BLOOFINZ-IO
largerWorkCitation
project
eng; USA
oceans
Eastern Indian Ocean (Argo Basin)
113.9674
121.502794
-17.17
-13.002801
2022-01-31
2022-03-02
Eastern Indian Ocean, Indonesian Throughflow area, and the Gulf of Mexico
0
BCO-DMO catalogue of parameters from Larval fish counts and larval tuna counts with corresponding plankton net type and geographic coordinates from R/V Roger Revelle cruise RR2201 (BLOOFINZ-IO, January-March 2022) in the Argo Basin region off NW Australia
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/958781.rdf
Name: Event_No
Units: unitless
Description: <p>Event number from event logger on Roger Revelle</p>
http://lod.bco-dmo.org/id/dataset-parameter/958782.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time (UTC) in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/958783.rdf
Name: Date
Units: unitless
Description: <p>Date</p>
http://lod.bco-dmo.org/id/dataset-parameter/958784.rdf
Name: Time
Units: unitless
Description: <p>Time (UTC)</p>
http://lod.bco-dmo.org/id/dataset-parameter/958785.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Decimal latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/958786.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Decimal latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/958787.rdf
Name: Tow
Units: unitless
Description: <p>Tuna team tow number 001-189 in sequential order</p>
http://lod.bco-dmo.org/id/dataset-parameter/958788.rdf
Name: Net_Type
Units: unitless
Description: <p>Type of net: Bongo90 (0.90 x 0.90 cm), Widow (0.9 x 0.9 cm), or Neuston (1m2)</p>
http://lod.bco-dmo.org/id/dataset-parameter/958789.rdf
Name: Mesh
Units: microns
Description: <p>Mesh size: 505 or 1000</p>
http://lod.bco-dmo.org/id/dataset-parameter/958790.rdf
Name: Side
Units: unitless
Description: <p>Right, R or Left, L. Neustons will have a blank side.</p>
http://lod.bco-dmo.org/id/dataset-parameter/958791.rdf
Name: Tow_Depth
Units: meters (m)
Description: <p>Maximum depth of Net_type</p>
http://lod.bco-dmo.org/id/dataset-parameter/958792.rdf
Name: Tow_Vol
Units: cubic meters (m3)
Description: <p>Volume filtered by net calculated by multiplying mouth area x flowmeter counts x constant</p>
http://lod.bco-dmo.org/id/dataset-parameter/958793.rdf
Name: Sample_ID
Units: unitless
Description: <p>Unique six digit identifier for Net_Type</p>
http://lod.bco-dmo.org/id/dataset-parameter/958794.rdf
Name: Cycle
Units: unitless
Description: <p>RR2201 experimental cycles 1-4</p>
http://lod.bco-dmo.org/id/dataset-parameter/958795.rdf
Name: Sample_Status
Units: unitless
Description: <p>"Tunas (ship)" indicates that all Scombrid (Tuna family) were counted aboard the ship; "Tunas (ship, lab)" indicates that all Scombrids were counted first aboard the sihp and completed in the lab; "All (ship,lab)" indicates all fish larvae counted in 100% of sample starting on the ship and completed in the lab; "All (ship, lab 1/4)" indicates tunas were counted and removed on the ship and the lab while all other larval fish were counted in 1/4 of the sample in lab.</p>
http://lod.bco-dmo.org/id/dataset-parameter/958796.rdf
Name: SBT_Count
Units: individuals
Description: <p>Southern bluefin tuna (SBT) Thunnus maccoyii counts</p>
http://lod.bco-dmo.org/id/dataset-parameter/958797.rdf
Name: ALB_Count
Units: individuals
Description: <p>Albacore tuna (ALB) Thunnus alalunga counts</p>
http://lod.bco-dmo.org/id/dataset-parameter/958798.rdf
Name: YFT_Count
Units: individuals
Description: <p>Yellowfin tuna (YFT) Thunnus albacares counts</p>
http://lod.bco-dmo.org/id/dataset-parameter/958799.rdf
Name: BET_Count
Units: individuals
Description: <p>Bigeye tuna (BET) Thunnus obesus counts</p>
http://lod.bco-dmo.org/id/dataset-parameter/958800.rdf
Name: Thun_Count
Units: individuals
Description: <p>Scombridae larvae, tunas identified to genus level only, Thunnus Sp.</p>
http://lod.bco-dmo.org/id/dataset-parameter/958801.rdf
Name: SKJ_Count
Units: individuals
Description: <p>Skipjack tuna (SKJ) Katsuwonus pelamis counts</p>
http://lod.bco-dmo.org/id/dataset-parameter/958802.rdf
Name: Tuna_Count
Units: individuals
Description: <p>Scombridae (tuna) counts, includes all larvae from the genus Thunnus and Katsuwonus</p>
http://lod.bco-dmo.org/id/dataset-parameter/958803.rdf
Name: Fish_Count
Units: individuals
Description: <p>Larval fish counts, "lab 1/4" are counts x 4</p>
http://lod.bco-dmo.org/id/dataset-parameter/958804.rdf
Name: SBT_1000m3
Units: individuals per 1000 cubic meters
Description: <p>SBT_Count / Tow_Vol x 1000</p>
http://lod.bco-dmo.org/id/dataset-parameter/958805.rdf
Name: ALB_1000m3
Units: individuals per 1000 cubic meters
Description: <p>ALB_Count / Tow_Vol x 1000</p>
http://lod.bco-dmo.org/id/dataset-parameter/958806.rdf
Name: YFT_1000m3
Units: individuals per 1000 cubic meters
Description: <p>YFT_Count / Tow_Vol x 1000</p>
http://lod.bco-dmo.org/id/dataset-parameter/958807.rdf
Name: BET_1000m3
Units: individuals per 1000 cubic meters
Description: <p>BET_Count / Tow_Vol x 1000</p>
http://lod.bco-dmo.org/id/dataset-parameter/958808.rdf
Name: Thun_1000m3
Units: individuals per 1000 cubic meters
Description: <p>Thun_Count / Tow_Vol x 1000</p>
http://lod.bco-dmo.org/id/dataset-parameter/958809.rdf
Name: SKJ_1000m3
Units: individuals per 1000 cubic meters
Description: <p>SKJ _Count / Tow_Vol x 1000</p>
http://lod.bco-dmo.org/id/dataset-parameter/958810.rdf
Name: Tuna_1000m3
Units: individuals per 1000 cubic meters
Description: <p>Tuna _Count / Tow_Vol x 1000</p>
http://lod.bco-dmo.org/id/dataset-parameter/958811.rdf
Name: Fish_1000m3
Units: individuals per 1000 cubic meters
Description: <p>Fish _Count / Tow_Vol x 1000</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
34300
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/d58f3516-a389-4403-b0a6-437ab83880f3/content
download
https://doi.org/10.26008/1912/bco-dmo.958726.1
download
onLine
dataset
<p>Larval tuna sampling was done by three net systems fished in the upper 25-30 meters (m) of the water column. The main gear utilized was the Bongo90. Standard larval sampling was done with 10-minute (~2 knots) oblique hauls in the upper 25 m of the water column using a dual 90-centimeter (cm) diameter bongo frame with 505-micrometer (μm) mesh nets (Laiz-Carrion et al., 2015). Filtered volumes were calculated with mechanical flowmeters (2030R, General Oceanics Inc.) centered in each net mouth. Bongo90 tows were generally done with a smaller "mini-bongo" net (20-cm diameter mouth; 200 and 50-μm mesh nets with flowmeters) attached above the larger nets to sample zooplankton prey (Shiroza et al., 2021). A second net, the 'Black Widow' was the name given to a modified Bongo90 frame with black-dyed nets (1000 µm mesh), was towed at higher speed at night with a depressor (and without the mini-bongo) to collect larger, more evasive larvae. The Black Widow net was lost due to a break in the Kevlar line on tow 54 (Cycle 2, 9 February).The third net utilized was the neuston net. The neuston was a square single-frame coarse-mesh net (1 square meter, with 1000-µm mesh) that was towed at the sea surface. Although operated at lower speed (≤2 kts) than the Black Widow, it was also successful in collecting larger larvae after the widow was lost.</p>
<p>Freshly collected samples (one side of the bongo net collections or the Neuston) were put on ice and live sorted and sized to species and developmental stage (preflexion, flexion, postflexion) at-sea following Richards et al., 2005. Sorted specimens were numbered and either frozen or EtOH preserved in individual vials for later analyses of gut contents (feeding), otolith microstructure (growth), and isotopic composition (trophic position). The second sides of bongo net collections were preserved whole by a multi-step process that replaced residual water with (ethyl ethanol 95%, EtOH) after 24 hours. Shipboard sorting yielded over 3700 larval tuna specimens, dominated (~86%) by southern bluefin tuna (SBT,<em> Thunnus maccoyii)</em> which were most abundant along the southern margin of the Argo Basin compared to the central basin. Yellowfin tuna (YFT, <em>T. albacares</em>) was second in relative abundance (~7%), and the remainder were relatively equally divided among T. albacore tuna (ALB, T<em>. alalunga</em> ), big eye tuna (BET, <em>T. obesus),</em> and skipjack tuna (SKJ, <em>Katsuwonus pelamis)</em>. A mass of freshly spawned eggs sampled on one occasion and subsequently confirmed to be SBT by genetic analysis will be important in isotopic interpretation of larval trophic position by providing a correction for the maternal contribution.</p>
<p>Laboratory analysis at the University of Miami was conducted by first measuring plankton displacement volume and wet weight of plankton samples. Next, the preservative (EtOH) was buffered by adding 125 milliliters (mL) of Tris buffer to a 5-gallon carboy. All plankton jars were transferred into buffered EtOH, and subsequent vials contained buffered EtOH. Next, a thorough examination of the right side (R) Bongo90 plankton samples was conducted under 0.63 - 4x magnification using a stereomicroscope to make sure that shipboard sorting did not miss any scombrid larvae. All larval fish were removed from a subset (n=67) of tows and from a 1/4 aliquot split (n=21) of Bongo90 samples. Scombrid larvae were identified to the lowest taxonomic level possible, and were labelled as '<em>Thunnus Sp.'</em> when species-level identification was not possible. Scombrid larvae were removed from an additional 38 sets of tows without removing other taxa to expedite sample processing of scombrid larvae for collaborative projects.</p>
<p>Over 2400 <em>Thunnus</em> larvae were individually examined, assigned a developmental stage, and given a unique identification number; a subset (n=480) was measured and photographed. Larval scombrids were shipped to collaborators for stomach content identification, prey preference analysis, otolith removal for ageing, genetics (PCR multiplex-identification, genome mapping), isotope analysis, and compound-specific isotope analysis.</p>
Specified by the Principal Investigator(s)
<p>Larval counts were logged in a datasheet and uploaded to a central database in Excel. Quality control was performed multiple times at each step of the lab data collection process.</p>
Specified by the Principal Investigator(s)
- Imported original file "OCE-1851395_Malca_Larvaltunacounts.xlsx" into the BCO-DMO system.
- Created columns for date and time from the Event_No column.
- Created column for date-time (UTC) in ISO8601 format.
- Saved the final file as "958726_v1_bloofinz-io_larval_tuna_counts_and_abundances.csv".
(Note that in the final file, missing data or N/A data are blank/empty.)
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Bongo90
Bongo90
PI Supplied Instrument Name: Bongo90 PI Supplied Instrument Description:Bongo90 (a dual 90-cm diameter bongo frame with 505-μm mesh nets) and 'Black Widow', a modified Bongo90 frame with black-dyed nets with 1000 µm mesh. Instrument Name: Bongo Net Instrument Short Name:Bongo Net Instrument Description: A Bongo Net consists of paired plankton nets, typically with a 60 cm diameter mouth opening and varying mesh sizes, 10 to 1000 micron. The Bongo Frame was designed by the National Marine Fisheries Service for use in the MARMAP program. It consists of two cylindrical collars connected with a yoke so that replicate samples are collected at the same time. Variations in models are designed for either vertical hauls (OI-2500 = NMFS Pairovet-Style, MARMAP Bongo, CalVET) or both oblique and vertical hauls (Aquatic Research). The OI-1200 has an opening and closing mechanism that allows discrete "known-depth" sampling. This model is large enough to filter water at the rate of 47.5 m3/minute when towing at a speed of two knots. More information: Ocean Instruments, Aquatic Research, Sea-Gear Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/NETT0009/
mechanical flowmeters (2030R, General Oceanics Inc.)
mechanical flowmeters (2030R, General Oceanics Inc.)
PI Supplied Instrument Name: mechanical flowmeters (2030R, General Oceanics Inc.) PI Supplied Instrument Description:The Bongo90, Black Widow, and the Neuston net had mechanical flowmeters (2030R, General Oceanics Inc.) centered in the middle to measure the amount of water filtered by each net. Instrument Name: Flow Meter Instrument Short Name:Flow Meter Instrument Description: General term for a sensor that quantifies the rate at which fluids (e.g. water or air) pass through sensor packages, instruments, or sampling devices. A flow meter may be mechanical, optical, electromagnetic, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/388/
neuston net
neuston net
PI Supplied Instrument Name: neuston net PI Supplied Instrument Description:the neuston was a square single-frame coarse-mesh net (1 m2, with 1000-µm mesh) Instrument Name: Neuston Net Instrument Short Name:Neuston Net Instrument Description: Neuston Nets are nets that collect zooplankton that live in the top few centimeters of the sea surface (the neuston layer). This specialized net has a rectangular mouth opening usually 2 or 3 times as wide as deep, i.e. 1 meter by 1/2 meter or 60 cm by 20 cm, with sometimes hollow piping construction to aid in flotation. They are generally towed half submerged at 1-2 kts from the side of the vessel on a boom to avoid the ship's wake. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/NETT0110/
Sensus Ultra U-15763, Reefnet Inc.
Sensus Ultra U-15763, Reefnet Inc.
PI Supplied Instrument Name: Sensus Ultra U-15763, Reefnet Inc. PI Supplied Instrument Description:Depth was measured initially live with a cNODE transpoder (MINI34), however the depth reading was not reliable, and a dive logging device (Sensus Ultra U-15763, Reefnet Inc.) was attached to the Bongo90 frame. Depth was recorded after the tow was completed, and the amount of winch cable released was recorded at each cast. Instrument Name: Reefnet Sensus Ultra Instrument Short Name: Instrument Description: The ReefNet Sensus Ultra is a compact temperature and depth logging sensor designed for use as a dive computer. Depths between 0 and 500 feet are measured with precision better than 0.5 inches of water, and accuracy of +/-12 inches. Temperature is measured between -20 degrees C and + 40 degrees C, with precision of 0.01 degrees C, and accuracy of +/-0.8 degrees C. The sampling interval is configurable from 1 second to over 18 hours, with storage capacity of one year at 60-second sampling resolution.
Cruise: RR2201
RR2201
R/V Roger Revelle
Community Standard Description
International Council for the Exploration of the Sea
R/V Roger Revelle
vessel
RR2201
Michael R. Landry
University of California-San Diego Scripps
http://hdl.handle.net/1834/43464
Report describing RR2201
R/V Roger Revelle
Community Standard Description
International Council for the Exploration of the Sea
R/V Roger Revelle
vessel