http://lod.bco-dmo.org/id/dataset/959971
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-04-29
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Energy reserve and stable isotope data from 3 species of Australian coral exposed to increased temperature and nutrients treatment in 2008
2025-09-22
publication
2025-09-22
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-09-22
publication
https://doi.org/10.26008/1912/bco-dmo.959971.1
Stephen Levas
Ohio State University
principalInvestigator
Andréa G. Grottoli
Ohio State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Levas, S., Grottoli, A. G. (2025) Energy reserve and stable isotope data from 3 species of Australian coral exposed to increased temperature and nutrients treatment in 2008. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-09-22 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.959971.1 [access date]
Winter temp and nutrient coral measurements Dataset Description: Methods and Sampling: <p>Twelve small colonies each of <em>Montipora monasteriata</em>, <em>Acropora muricata</em>, and <em>Pocillopora damicornis</em> were collected at 4-5m depth from the reefs at Heron Island, Queensland, Australia (23.4423°S, 151.9148°E) and placed in treatment tanks.</p>
<p>Daily temperature was recorded using Hobo temperature loggers every minute within the treatment tank. Chl <em>a, </em>total soluble lipid, soluble animal protein, and soluble animal carbohydrate concentrations, were each measured on a 1cm<sup>2</sup> cored plugs of <em>M. monasteriata </em>and from 1cm long branch tips of <em>A. muricata</em> and <em>P. damicornis </em>from each ramet. Each measurement was made on whole coral samples (skeleton, animal tissue, and endosymbiotic algae) that were ground with a mortar and pestle and normalized to total ash-free dry tissue biomass of the organic fraction (animal tissue and endosymbiotic algae). Chl <em>a</em> was extracted using methods modified from Jeffrey and Humphrey (1975). Total soluble lipids were extracted using methods described in Rodrigues and Grottoli (2007), while soluble animal carbohydrate and protein concentrations were measured using the methods modified from Dubois et al. (1956) and Smith et al. (1985), respectively, as described in Levas et al. (2018). Biomass was measured according to methods outlined in McLachlan et al. (2020).</p>
<p>Coral fragments were airbrushed to remove all tissue from the skeleton. The host tissue and endosymbionts were separated by centrifugation and filtered onto prebaked GF/F filters. Animal host tissue and endosymbiotic algal fraction δ<sup>15</sup>N values (δ<sup>15</sup>N<sub>h</sub>&nbsp;and δ<sup>15</sup>N<sub>e</sub>, respectively) were reported relative to air (δ<sup>15</sup>N = per mil deviation of the ratio of stable nitrogen isotopes&nbsp;<sup>15</sup>N:<sup>14</sup>N relative to air). Animal host tissue and endosymbiotic algal fraction δ<sup>13</sup>C values (δ<sup>13</sup>C<sub>h</sub>&nbsp;and δ<sup>13</sup>C<sub>e</sub>, respectively) were reported relative to Vienna Peedee Belemnite Limestone standard (δ<sup>13</sup>C = per mil deviation of the ratio of stable carbon isotopes&nbsp;<sup>13</sup>C:<sup>12</sup>C relative to V-PDB). Repeated measurements of internal standards (n = 20) had a standard deviation of ± 0.14‰ for organic δ<sup>15</sup>N and ± 0.07‰ for organic δ<sup>13</sup>C. &nbsp;δ<sup>15</sup>N and δ<sup>13</sup>C values were determined using a Costech Elemental Analyzer where the resulting N<sub>2</sub>&nbsp;and CO<sub>2</sub>&nbsp;gases were analyzed for δ<sup>15</sup>N and δ<sup>13</sup>C with a ThermoFisher Delta IV stable isotope ratio mass spectrometer (IRMS) via a Conflo II interface in the Grottoli lab at the Ohio State University.</p>
Funding provided by NSF Office of International Science and Engineering (NSF OISE) Award Number: OISE-0813231 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0813231
completed
Stephen Levas
Ohio State University
levass@uww.edu
pointOfContact
Andréa G. Grottoli
Ohio State University
614-292-5782
School of Earth Sciences 125 South Oval Mall
Columbus
OH
43210
United States
grottoli.1@osu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
ID
Species
Temp
Nutrient
T_Chla
d13C_Animal
d13C_Zoox
d13C_Animal_d13C_Zoox
d15N_Animal
d15N_Zoox
d15N_Animal_d15N_Zoox
Protein
Carbs
Lipid
Total_EnRes
Total_Biomass
AphiaID
LSID
Costech Elemental Analyzer
ThermoFisher Delta IV stable isotope ratio mass spectrometer (IRMS)
Hobo temperature loggers
theme
None, User defined
sample identification
species scientific name (binomial)
treatment
chlorophyll a
d13C measured in biota
d15N measured in biota
proteins
carbohydrates
lipids in biota
energy_density
dry_weight_biomass
taxon_code
Life Science Identifier (LSID)
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Isotope-ratio Mass Spectrometer
scale or balance
Temperature Logger
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
EAPSI: How Do Coastal Eutrophication and Elevated Ocean Temperature Impact On Reef Corals
https://osprey.bco-dmo.org/project/960009
EAPSI: How Do Coastal Eutrophication and Elevated Ocean Temperature Impact On Reef Corals
<p>2008 EAPSI Fellowship</p>
<p>This award supports a U.S. graduate student to conduct an individual research project at one of seven locations in East Asia and the Pacific region (Australia, China, Japan, Korea, New Zealand, Singapore, Taiwan). The research project will provide the student with a first-hand mentored research experience, an introduction to science and science policy infrastructure, and an orientation to the culture and language of the location. The primary goals of the East Asia Summer Institute program are to expose students to science and engineering in the context of a research laboratory, and to initiate early-career professional relationships that will foster research collaborations with foreign counterparts in the future.</p>
EAPSI Eutrophication Temperature
largerWorkCitation
project
eng; USA
biota
oceans
151.9148
151.9148
-23.4423
-23.4423
2008-07-20
2008-08-31
East Asia and the Pacific region (Australia, China, Japan, Korea, New Zealand, Singapore, Taiwan)
0
BCO-DMO catalogue of parameters from Energy reserve and stable isotope data from 3 species of Australian coral exposed to increased temperature and nutrients treatment in 2008
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/972158.rdf
Name: ID
Units: unitless
Description: <p>ID used to identify coral based on species (first two letters), temperature treatment (second pair of letters), nutrient regime (third pair of letters) and then colony ID (four numbers)</p>
http://lod.bco-dmo.org/id/dataset-parameter/972159.rdf
Name: Species
Units: unitless
Description: <p>Species ID (1=<em>Montipora monasteriata</em>, 2=<em>Acropora muricata</em>, 3=<em>Pocillopora damicornis</em>)</p>
http://lod.bco-dmo.org/id/dataset-parameter/972160.rdf
Name: Temp
Units: unitless
Description: <p>Temperature Treatment (1= control/NB, 2 = increased temperature/BL); NB refers to Non-bleached coral and BL refers to Bleached coral</p>
http://lod.bco-dmo.org/id/dataset-parameter/972161.rdf
Name: Nutrient
Units: unitless
Description: <p>Nutrient Regime (1=Low nutrients, 2= high nutrients)</p>
http://lod.bco-dmo.org/id/dataset-parameter/972162.rdf
Name: T_Chla
Units: ug/cm²
Description: <p>Total Chlorophyll per Area</p>
http://lod.bco-dmo.org/id/dataset-parameter/972163.rdf
Name: d13C_Animal
Units: per mil
Description: <p>d13C for the Animal tissue</p>
http://lod.bco-dmo.org/id/dataset-parameter/972164.rdf
Name: d13C_Zoox
Units: per mil
Description: <p>d13C for the Endosymbiont</p>
http://lod.bco-dmo.org/id/dataset-parameter/972165.rdf
Name: d13C_Animal_d13C_Zoox
Units: per mil
Description: <p>d13C for the animal tissue subtracted by the d13C of the endosymbiont tissue</p>
http://lod.bco-dmo.org/id/dataset-parameter/972166.rdf
Name: d15N_Animal
Units: per mil
Description: <p>d15N fo the Animal tissue</p>
http://lod.bco-dmo.org/id/dataset-parameter/972167.rdf
Name: d15N_Zoox
Units: per mil
Description: <p>d15N for the Endosymbiont tissue</p>
http://lod.bco-dmo.org/id/dataset-parameter/972168.rdf
Name: d15N_Animal_d15N_Zoox
Units: per mil
Description: <p>d15N for the animal tissue subtracted by the d15N of the endosymbiont tissue</p>
http://lod.bco-dmo.org/id/dataset-parameter/972169.rdf
Name: Protein
Units: Joules per mg ash free dry weight
Description: <p>Total protein concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/972170.rdf
Name: Carbs
Units: Joules per mg ash free dry weight
Description: <p>Total carbohydrate concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/972171.rdf
Name: Lipid
Units: Joules per mg ash free dry weight
Description: <p>Total Lipid concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/972172.rdf
Name: Total_EnRes
Units: Joules per mg ash free dry weight
Description: <p>Total energy reserves (protein+lipid+carbs)</p>
http://lod.bco-dmo.org/id/dataset-parameter/972173.rdf
Name: Total_Biomass
Units: mg/cm2
Description: <p>Total biomas</p>
http://lod.bco-dmo.org/id/dataset-parameter/972174.rdf
Name: AphiaID
Units: unitless
Description: <p>AphiaID of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/972175.rdf
Name: LSID
Units: unitless
Description: <p>LSID of sample</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
16908
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/4f67c424-d6d7-4e11-b279-e472cb002817/content
download
https://doi.org/10.26008/1912/bco-dmo.959971.1
download
onLine
dataset
<p>Twelve small colonies each of <em>Montipora monasteriata</em>, <em>Acropora muricata</em>, and <em>Pocillopora damicornis</em> were collected at 4-5m depth from the reefs at Heron Island, Queensland, Australia (23.4423°S, 151.9148°E) and placed in treatment tanks.</p>
<p>Daily temperature was recorded using Hobo temperature loggers every minute within the treatment tank. Chl <em>a, </em>total soluble lipid, soluble animal protein, and soluble animal carbohydrate concentrations, were each measured on a 1cm<sup>2</sup> cored plugs of <em>M. monasteriata </em>and from 1cm long branch tips of <em>A. muricata</em> and <em>P. damicornis </em>from each ramet. Each measurement was made on whole coral samples (skeleton, animal tissue, and endosymbiotic algae) that were ground with a mortar and pestle and normalized to total ash-free dry tissue biomass of the organic fraction (animal tissue and endosymbiotic algae). Chl <em>a</em> was extracted using methods modified from Jeffrey and Humphrey (1975). Total soluble lipids were extracted using methods described in Rodrigues and Grottoli (2007), while soluble animal carbohydrate and protein concentrations were measured using the methods modified from Dubois et al. (1956) and Smith et al. (1985), respectively, as described in Levas et al. (2018). Biomass was measured according to methods outlined in McLachlan et al. (2020).</p>
<p>Coral fragments were airbrushed to remove all tissue from the skeleton. The host tissue and endosymbionts were separated by centrifugation and filtered onto prebaked GF/F filters. Animal host tissue and endosymbiotic algal fraction δ<sup>15</sup>N values (δ<sup>15</sup>N<sub>h</sub>&nbsp;and δ<sup>15</sup>N<sub>e</sub>, respectively) were reported relative to air (δ<sup>15</sup>N = per mil deviation of the ratio of stable nitrogen isotopes&nbsp;<sup>15</sup>N:<sup>14</sup>N relative to air). Animal host tissue and endosymbiotic algal fraction δ<sup>13</sup>C values (δ<sup>13</sup>C<sub>h</sub>&nbsp;and δ<sup>13</sup>C<sub>e</sub>, respectively) were reported relative to Vienna Peedee Belemnite Limestone standard (δ<sup>13</sup>C = per mil deviation of the ratio of stable carbon isotopes&nbsp;<sup>13</sup>C:<sup>12</sup>C relative to V-PDB). Repeated measurements of internal standards (n = 20) had a standard deviation of ± 0.14‰ for organic δ<sup>15</sup>N and ± 0.07‰ for organic δ<sup>13</sup>C. &nbsp;δ<sup>15</sup>N and δ<sup>13</sup>C values were determined using a Costech Elemental Analyzer where the resulting N<sub>2</sub>&nbsp;and CO<sub>2</sub>&nbsp;gases were analyzed for δ<sup>15</sup>N and δ<sup>13</sup>C with a ThermoFisher Delta IV stable isotope ratio mass spectrometer (IRMS) via a Conflo II interface in the Grottoli lab at the Ohio State University.</p>
Specified by the Principal Investigator(s)
<p>The 1 minute temperature data was averaged over the 24hr period to get a daily temperature.&nbsp;</p>
Specified by the Principal Investigator(s)
- Imported "physiological_parameters_heron_island_coral_2008.xlsx" into BCO-DMO system
- Rounded parameters 'Protein (J mg afdw)', 'Carbs (J mg/afdw)', and 'Lipid (J mg afdw)' to 6 digits (as indicated in Excel formatting)
- Rounded 'Total EnRes (J mg afdw)' to 5 digits (as indicated in Excel formatting)
- Rounded 'Total Biomass (mg/cm2)' to 1 digit (as indicated in Excel formatting)
- Renamed fields to comply with BCO-DMO naming conventions, removing units, special characters, and spaces
- Added accepted AphiaID and LSID for host species in "AphiaID_accepted" and "LSID"
- Exported file as "959971_v1_winter_temp_nutrient_coral.csv"
Scientific names in the data were checked using World Register of Marine Species (WoRMS) Taxon Match. All scientific names in the data are valid and accepted names as of 2025-08-07.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Costech Elemental Analyzer
Costech Elemental Analyzer
PI Supplied Instrument Name: Costech Elemental Analyzer PI Supplied Instrument Description:δ15N and δ13C values were determined using a Costech Elemental Analyzer where the resulting N2 and CO2 gases were analyzed for δ15N and δ13C with a ThermoFisher Delta IV stable isotope ratio mass spectrometer (IRMS) via a Conflo II interface in the Grottoli lab at the Ohio State University. Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
ThermoFisher Delta IV stable isotope ratio mass spectrometer (IRMS)
ThermoFisher Delta IV stable isotope ratio mass spectrometer (IRMS)
PI Supplied Instrument Name: ThermoFisher Delta IV stable isotope ratio mass spectrometer (IRMS) PI Supplied Instrument Description:δ15N and δ13C values were determined using a Costech Elemental Analyzer where the resulting N2 and CO2 gases were analyzed for δ15N and δ13C with a ThermoFisher Delta IV stable isotope ratio mass spectrometer (IRMS) via a Conflo II interface in the Grottoli lab at the Ohio State University. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
PI Supplied Instrument Name: PI Supplied Instrument Description:Each measurement was made on whole coral samples (skeleton, animal tissue, and endosymbiotic algae) that were ground with a mortar and pestle and normalized to total ash-free dry tissue biomass of the organic fraction (animal tissue and endosymbiotic algae). Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/
Hobo temperature loggers
Hobo temperature loggers
PI Supplied Instrument Name: Hobo temperature loggers PI Supplied Instrument Description:Daily temperature was recorded using Hobo temperature loggers every minute within the treatment tank. Instrument Name: Temperature Logger Instrument Short Name: Instrument Description: Records temperature data over a period of time.