http://lod.bco-dmo.org/id/dataset/961706 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2025-05-13 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Stable isotope compositions (carbon, nitrogen, and sulfur) of muscle tissue in three species of fish from the Matagorda Bay region of Texas in the northwestern Gulf of Mexico from 2021 to 2023 2025-05-14 publication 2025-05-14 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2025-08-08 publication https://doi.org/10.26008/1912/bco-dmo.961706.1 Benjamin Walther Texas A&M, Corpus Christi principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Walther, B., Oster, J. (2025) Stable isotope compositions (carbon, nitrogen, and sulfur) of muscle tissue in three species of fish from the Matagorda Bay region of Texas in the northwestern Gulf of Mexico from 2021 to 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-05-14 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.961706.1 [access date] Texas fish muscle stable isotopes Dataset Description: Methods and Sampling: &lt;p&gt;Fish collection locations and associated information are found in an accompanying dataset for this project: &lt;a href=&quot;https://www.bco-dmo.org/dataset/916418&quot; target=&quot;_blank&quot;&gt;https://www.bco-dmo.org/dataset/916418&lt;/a&gt;.&lt;/p&gt; &lt;p&gt;Fish carcasses were obtained from anglers at boat docks, fish cleaning stations, or other locations in the region. Fish were also obtained from bait shops where the proprietors verified the fish were sourced from the Matagorda Bay study region. These fish will be dissected to subsample white muscle tissue from each fish (skin and scales removed). Fish muscle tissue was dehydrated, pulverized to a fine powder, and packed into individual tin capsules for stable isotope analyses. All isotope analyses were conducted at the University of California, Davis Stable Isotope Facility. Analytical methods follow those described by Walther &amp;amp; Torrance (2024) and Valenza et al. (2025).&lt;/p&gt; &lt;p&gt;Additional text describing methods below are from &lt;a href=&quot;https://stableisotopefacility.ucdavis.edu/&quot; target=&quot;_blank&quot;&gt;https://stableisotopefacility.ucdavis.edu/&lt;/a&gt;&lt;/p&gt; &lt;p&gt;Samples were analyzed for 13C and 15N isotopes using a PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK). Samples are combusted at 1000 degrees Celsius (°C) in a reactor packed with chromium oxide and silvered copper oxide. Following combustion, oxides are removed in a reduction reactor (reduced copper at 650°C). The helium carrier then flows through a water trap (magnesium perchlorate and phosphorous pentoxide). N2 and CO2 are separated on a Carbosieve GC column (65°C, 65 milliliters per minute (mL/min)) before entering the IRMS. During analysis, samples are interspersed with several replicates of at least four different laboratory reference materials. The long-term standard deviation is 0.2 ‰ for 13C and 0.3 ‰ for 15N. The final delta values, delivered to the customer, are expressed relative to international standards VPDB (Vienna Pee Dee Belemnite) and Air for carbon and nitrogen, respectively.&lt;/p&gt; &lt;p&gt;Stable isotope ratios of 34S in solid samples are measured using an Elementar vario ISOTOPE cube interfaced to an Isoprime PrecisION IRMS (Cheadle Hume, Stockport, UK). Samples are combusted at 1000°C in a reactor packed with tungsten oxide and elemental copper. Immediately following combustion, sample gases are reduced with elemental copper at 900°C and subsequently pass through a buffering reactor filled with quartz chips held at 900°C. SO2 and CO2 are then separated by purge and trap, allowing for full separation and peak focusing. Following separation, the SO2 adsorption trap is heated and the sample SO2 passes directly to the IRMS for measurement.&lt;/p&gt; &lt;p&gt;During analysis, samples are interspersed with replicates of several laboratory reference materials to monitor and allow for correction of any potential variation in drift and linearity. Final 34S delta values are obtained after adjusting the provisional measurements such that correct 34S delta values for laboratory quality assurance materials are obtained. The long-term reproducibility of this method is ± 0.4 ‰.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1924011 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1924011 completed Benjamin Walther Texas A&M, Corpus Christi 361-825-4168 6300 Ocean Drive Unit # 5858 Corpus Christi TX 78412 USA benjamin.walther@tamucc.edu pointOfContact asNeeded Dataset Version: 1 Unknown FishID Duplicate Species_Code Species C13 N15 S34 Station_Latitude Station_Longitude Date_Collected Elementar vario ISOTOPE cube PDZ Europa ANCA-GSL elemental analyzer Isoprime PrecisION IRMS PDZ Europa 20-20 isotope ratio mass spectrometer theme None, User defined sample identification replicate species scientific name (binomial) d13C measured in biota d15N measured in biota d34S_bio latitude longitude date featureType BCO-DMO Standard Parameters Elemental Analyzer Elemental Analyzer Isotope-ratio Mass Spectrometer PDZ Europa 20-20 isotope ratio mass spectrometer instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Shifting the Hypoxia Paradigm – New Directions to Explore the Spread and Impacts of Ocean/Great Lakes Deoxygenation https://osprey.bco-dmo.org/project/770460 Collaborative Research: Shifting the Hypoxia Paradigm – New Directions to Explore the Spread and Impacts of Ocean/Great Lakes Deoxygenation <p><em>NSF Award Abstract:</em><br /> Ocean oxygen loss (deoxygenation) is increasing due to climate warming. This warming, together with nutrient loading, is causing many marine and freshwater systems to experience increasing episodes of hypoxia (low oxygen) of greater duration and intensity. Impacts on fish and fisheries have been difficult to quantify; direct observation has been challenged by a lack of long-term exposure indicators. This team has successfully refined the use of fish chemical biomarkers in fish otoliths (earstones) to directly assess lifetime hypoxia exposure in fishes. This project will those findings to look for additional biomarkers and models, to expand our understanding of how hypoxia affects fish and their food webs, contaminant transfers, and ecosystem services including economic impacts. The project includes a unique way of training students in science communication, posing the question: What forms of media and "messaging strategies" about deoxygenation are most effective at raising public awareness and understanding? Students are developing entries for PlanetForward's Storyfest, which is a contest to tell compelling stories to foster environmental understanding and solutions. Students from historically underrepresented, economically disadvantaged backgrounds are particularly sought out to participate. The investigators will engage with regional, national, and international management agencies and other relevant stakeholder groups to share information.</p> <p>This project encompasses a novel, linked set of interdisciplinary studies of food webs, and ecosystem services assessment. The thematic questions explored in this project are: 1. How does hypoxia alter habitat use for fishes? 2. How does hypoxia-altered habitat use and habitat productivity change food webs? 3. How does hypoxia affect/enhance trophic transfer of methylmercury? 4. How do hypoxia-induced changes in food webs affect aquatic ecosystem services? The set of linked studies will employ chemical analyses of otoliths and eye lenses, combined with chemical analyses of muscle tissues (Questions 1 and 3), physiologically-structured food web modeling informed by monitoring time-series (Questions 2 and 4), and a scoping workshop to address ecosystem services (Question 4). The investigators are using a "trans-basin" comparative approach to system-specific responses, studying fishes in Lake Erie, the Baltic Sea, and a Gulf of Mexico estuary. They study three species from each system that represent different degrees of benthic reliance, to discern differential responses to the increasingly hypoxic environment. This research provides novel insight about variable biotic responses to oxygen loss and the impacts on ecosystem functioning.</p> HypoxiDigm / Project Breathless largerWorkCitation project eng; USA oceans -97.3201482 -91.80883333 27.6796764 29.009 2021-07-16 2023-07-01 Central Baltic Sea; Lake Erie; and Lavaca Bay, Texas 0 BCO-DMO catalogue of parameters from Stable isotope compositions (carbon, nitrogen, and sulfur) of muscle tissue in three species of fish from the Matagorda Bay region of Texas in the northwestern Gulf of Mexico from 2021 to 2023 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/961779.rdf Name: FishID Units: unitless Description: <p>Unique identifier number for each individual fish included in the project. IDs include a prefix of &quot;MB&quot; (Matagorda Bay) or &quot;PB&quot; (Project Breathless) followed by a unique sequence of digits.</p> http://lod.bco-dmo.org/id/dataset-parameter/961780.rdf Name: Duplicate Units: unitless Description: <p>Number indicating duplicate (1 or 2)</p> http://lod.bco-dmo.org/id/dataset-parameter/961781.rdf Name: Species_Code Units: unitless Description: <p>Code indicating whether fish was a Red Drum (&quot;RD&quot;), Atlantic Croaker (&quot;CR&quot;) or Southern Flounder (&quot;FL&quot;).</p> http://lod.bco-dmo.org/id/dataset-parameter/961782.rdf Name: Species Units: unitless Description: <p>Latin binomial (Genus species) of each individual collected</p> http://lod.bco-dmo.org/id/dataset-parameter/961783.rdf Name: C13 Units: permil (‰) Description: <p>Stable isotope ratio of carbon (d13C)</p> http://lod.bco-dmo.org/id/dataset-parameter/961784.rdf Name: N15 Units: permil (‰) Description: <p>Stable isotope ratio of nitrogen (d15N)</p> http://lod.bco-dmo.org/id/dataset-parameter/961785.rdf Name: S34 Units: permil (‰) Description: <p>Stable isotope ratio of sulfur (d34S)</p> http://lod.bco-dmo.org/id/dataset-parameter/961786.rdf Name: Station_Latitude Units: decimal degrees Description: <p>Collection location latitude in decimal degrees; positive values = North</p> http://lod.bco-dmo.org/id/dataset-parameter/961787.rdf Name: Station_Longitude Units: decimal degrees Description: <p>Collection location longitude in decimal degrees; negative values = West</p> http://lod.bco-dmo.org/id/dataset-parameter/961788.rdf Name: Date_Collected Units: unitless Description: <p>Date of fish collection</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 24064 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/be0efe85-a48d-4ebe-9f48-45e5285bd3c5/content download https://doi.org/10.26008/1912/bco-dmo.961706.1 download onLine dataset &lt;p&gt;Fish collection locations and associated information are found in an accompanying dataset for this project: &lt;a href=&quot;https://www.bco-dmo.org/dataset/916418&quot; target=&quot;_blank&quot;&gt;https://www.bco-dmo.org/dataset/916418&lt;/a&gt;.&lt;/p&gt; &lt;p&gt;Fish carcasses were obtained from anglers at boat docks, fish cleaning stations, or other locations in the region. Fish were also obtained from bait shops where the proprietors verified the fish were sourced from the Matagorda Bay study region. These fish will be dissected to subsample white muscle tissue from each fish (skin and scales removed). Fish muscle tissue was dehydrated, pulverized to a fine powder, and packed into individual tin capsules for stable isotope analyses. All isotope analyses were conducted at the University of California, Davis Stable Isotope Facility. Analytical methods follow those described by Walther &amp;amp; Torrance (2024) and Valenza et al. (2025).&lt;/p&gt; &lt;p&gt;Additional text describing methods below are from &lt;a href=&quot;https://stableisotopefacility.ucdavis.edu/&quot; target=&quot;_blank&quot;&gt;https://stableisotopefacility.ucdavis.edu/&lt;/a&gt;&lt;/p&gt; &lt;p&gt;Samples were analyzed for 13C and 15N isotopes using a PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK). Samples are combusted at 1000 degrees Celsius (°C) in a reactor packed with chromium oxide and silvered copper oxide. Following combustion, oxides are removed in a reduction reactor (reduced copper at 650°C). The helium carrier then flows through a water trap (magnesium perchlorate and phosphorous pentoxide). N2 and CO2 are separated on a Carbosieve GC column (65°C, 65 milliliters per minute (mL/min)) before entering the IRMS. During analysis, samples are interspersed with several replicates of at least four different laboratory reference materials. The long-term standard deviation is 0.2 ‰ for 13C and 0.3 ‰ for 15N. The final delta values, delivered to the customer, are expressed relative to international standards VPDB (Vienna Pee Dee Belemnite) and Air for carbon and nitrogen, respectively.&lt;/p&gt; &lt;p&gt;Stable isotope ratios of 34S in solid samples are measured using an Elementar vario ISOTOPE cube interfaced to an Isoprime PrecisION IRMS (Cheadle Hume, Stockport, UK). Samples are combusted at 1000°C in a reactor packed with tungsten oxide and elemental copper. Immediately following combustion, sample gases are reduced with elemental copper at 900°C and subsequently pass through a buffering reactor filled with quartz chips held at 900°C. SO2 and CO2 are then separated by purge and trap, allowing for full separation and peak focusing. Following separation, the SO2 adsorption trap is heated and the sample SO2 passes directly to the IRMS for measurement.&lt;/p&gt; &lt;p&gt;During analysis, samples are interspersed with replicates of several laboratory reference materials to monitor and allow for correction of any potential variation in drift and linearity. Final 34S delta values are obtained after adjusting the provisional measurements such that correct 34S delta values for laboratory quality assurance materials are obtained. The long-term reproducibility of this method is ± 0.4 ‰.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;Data are provided as reported by the analytical facility without additional processing. Duplicate samples from some fish were analyzed separately to assess within-fish variation. Those results are provided separately and indicated as duplicates within the dataset.&lt;/p&gt; Specified by the Principal Investigator(s) - Imported fish collection location data (file &quot;916418_v2_fish_collection_locations.csv&quot;) - Imported original file &quot;SIA_Data_Upload.xlsx&quot; into the BCO-DMO system. - Flagged &quot;NAN&quot; as a missing data value (missing data are empty/blank in the final CSV file). - Added the following columns from the fish collection location data file to this dataset by joining on Fish_ID: Station_Latitude, Station_Longitude, Date_Collected, Species. - Saved the final file as &quot;961706_v1_fish_muscle_stable_isotopes.csv&quot;. Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Elementar vario ISOTOPE cube Elementar vario ISOTOPE cube PI Supplied Instrument Name: Elementar vario ISOTOPE cube Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/ PDZ Europa ANCA-GSL elemental analyzer PDZ Europa ANCA-GSL elemental analyzer PI Supplied Instrument Name: PDZ Europa ANCA-GSL elemental analyzer Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/ Isoprime PrecisION IRMS Isoprime PrecisION IRMS PI Supplied Instrument Name: Isoprime PrecisION IRMS Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/ PDZ Europa 20-20 isotope ratio mass spectrometer PDZ Europa 20-20 isotope ratio mass spectrometer PI Supplied Instrument Name: PDZ Europa 20-20 isotope ratio mass spectrometer Instrument Name: PDZ Europa 20-20 isotope ratio mass spectrometer Instrument Short Name:Europa 20-20 IRMS Instrument Description: The PDZ Europa 20-20 is a dedicated continuous flow isotope ratio mass spectrometer for hyphenated stable isotope analyses able to measure 15N, 13C, 18O, and 34S in a host of applications. The analyzer has been purposely designed to measure 2H by continuous flow methodology and is also suitable to analyze the light stable isotopes in all the commonly measured gases: H2, N2, NO, N2O, O2, CO, CO2, SO, and SO2.