http://lod.bco-dmo.org/id/dataset/963393
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-05-31
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Cell counts exhibiting 'Selfish' uptake in the Western North Atlantic, in Danish coastal seawater, and abyssopelagic waters off the eastern coast of Japan under varying hydrostatic pressures, 2023-2024
2025-06-06
publication
2025-06-06
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-06-10
publication
https://doi.org/10.26008/1912/bco-dmo.963393.1
Carol Arnosti
University of North Carolina at Chapel Hill
principalInvestigator
Chad Lloyd
University of North Carolina at Chapel Hill
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lloyd, C., Hennessey, E., Ghobrial, S., Arnosti, C. (2025) Cell counts exhibiting 'Selfish' uptake in the Western North Atlantic, in Danish coastal seawater, and abyssopelagic waters off the eastern coast of Japan under varying hydrostatic pressures, 2023-2024. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-06-06 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.963393.1 [access date]
Combined Selfish Bacteria Cell Counts 2023-2024 Dataset Description: Methods and Sampling: <p>Water for incubation and filtration was collected via Niskin bottles mounted on a rosette, equipped with a CTD for Western North Atlantic, Danish Coastal Seawater, and Abyssopelagic Waters off the Eastern Coast of Japan.&nbsp;Additionally, in the Western North Atlantic, water was collected via an in-situ syringe-based sampler and incubator developed by the Danish Center for Hadal Research. &nbsp;</p>
<p>Niskin-collected water was used to measure cell counts and investigate selfish polysaccharide uptake under near-surface (0.1 MPa), or bathy- and abyssopelagic hydrostatic pressures (20, 40, 42 and 52 MPa). Water at each station was dispensed into smaller glass Duran bottles that were cleaned and pre-rinsed three times with water from the Niskin prior to dispensing. To 350 mL of bulk seawater or 100 mL of autoclaved seawater, substrate was added at 3.5 μM monomer-equivalent concentrations, except for fucoidan, which was added at 5 μM concentrations (a higher concentration was necessary for sufficient fluorescence signal). From these bottles, nine 6 mL Exetainer vials were filled with seawater for each substrate, replicate, and time point, and four 6 mL Exetainer vials were filled with autoclaved seawater to serve as a killed control. Sets of vials were then pressurized to either 0.1, 20, or 40 MPa in separate pressure vessels for each substrate and time point and stored in the dark at 4ºC for 0, 2, or 5 days.</p>
<p>When collected via an in-situ syringe sampler (ISS), glass syringes fitted to the sampler were preloaded with individual polysaccharide substrates to 3.5 μM monomer-equivalent concentrations or preloaded with autoclaved seawater to serve as a negative control (blank). &nbsp;Once lowered to the desired depth of 2000, 4000, 4200, or 5200 m (depending on station depth), half of the syringes were triggered so that they drew in surrounding water that mixed with the preloaded substrate or autoclaved seawater. &nbsp;The ISS was held at depth for approximately 24 hours of incubation. &nbsp;While still at depth, shortly prior to retrieval, the second half of the syringes were triggered to draw in the surrounding water. These syringes served to measure the quantity of selfish uptake occurring briefly at depth, and then during the upcast.&nbsp;</p>
<p>After sample incubation, approximately 25 mL of incubated sample was filtered through a 25 mm 0.2 µM nucleopore filter for molecular analysis, placed in cryovials, and promptly frozen at -80C.</p>
<p>Another approximately 25 mL of incubated sample was incubated with fixative for two hours, then filtered through a 25 mm 0.2 µM nucleopore filter. &nbsp;After filtration, the filters were dried in a laboratory hood, then each filter was labeled along the outer edge, placed in a Petri dish, and stored at -80C for total cell counts, and quantification of 'selfish' uptake.</p>
<p>Polysaccharide used for incubation:</p>
<ul>
<li>ara = arabinogalactan</li>
<li>chn = chondroitin sulfate</li>
<li>fuc = fucoidan</li>
<li>lam = laminarin</li>
<li>man = mannan</li>
<li>pul = pullulan</li>
<li>xyl = xylan</li>
<li>muc = mucin</li>
</ul>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2241720 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2241720
completed
Carol Arnosti
University of North Carolina at Chapel Hill
919-962-5754
Dept. of Marine Sciences 3117A Venable/Murray Hall
Chapel Hill
NC
27599-3300
USA
arnosti@email.unc.edu
pointOfContact
Chad Lloyd
University of North Carolina at Chapel Hill
cclloyd@unc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
deployment
station
latitude_n
longitude_e
ISO_DateTime_UTC
date
time_local
cast_number
depth_description
depth_actual
insitu_temp
sample_type
incubation_type
incubation_pressure
incubation_temp
unamended_amended
substrate
replicate
timepoint
timepoint_days
fields_of_view
SB_Abundance
Total_Abundance
Zeiss LSM780 with Airyscan (Carl Zeiss)
A rosette, equipped with a CTD
Zeiss AxioImager.Z2 microscope stand, Carl Zeiss - Fully automated epifluorescence microscope
In-situ syringe-based sampler and incubator - developed by the Danish Center for Hadal Research
Niskin bottles mounted on a rosette, equipped with a CTD
theme
None, User defined
cruise name
station
latitude
longitude
ISO_DateTime_UTC
date_local
time_local
cast
depth_comment
depth
water temperature at measurement depth
sample type
experiment type
water pressure
incubation or cultivation temperature (not ambient water temp)
sample description
replicate
time_point
time_elapsed
image_analysis
abundance
featureType
BCO-DMO Standard Parameters
Confocal Laser Scanning Microscope
CTD - profiler
Fluorescence Microscope
In-situ incubator
Niskin bottle
instrument
BCO-DMO Standard Instruments
AE2413
UM20231010
service
Deployment Activity
Northern Izu–Ogasawara Trench
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Pressure effects on microbially-catalyzed organic matter degradation in the deep ocean
https://osprey.bco-dmo.org/project/896812
Collaborative Research: Pressure effects on microbially-catalyzed organic matter degradation in the deep ocean
<p><em>NSF Award Abstract:</em><br />
Microbes are important players in the carbon cycle in the ocean. These organisms consume organic carbon and produce carbon dioxide in marine systems. Because the average depth of the ocean is 4000 m, microbes must work at high pressures typical of the deep ocean (>1000 m). Although high pressure is known to affect marine microbes, their carbon cycling activities have mostly been measured at surface ocean pressures. As a result, it remains unknown how closely these measurements reflect the activities of deep-sea microbes at high pressures. As a result of collaborations with scientists in Denmark and Germany, this project will be able to use special equipment to investigate the effects of high pressures on marine microbes and their carbon cycling activities. This work is necessary to quantify rates of carbon cycling and identify the microbes involved, especially in deep waters. The project will provide training for diverse undergraduate and graduate students, and a postdoc who will conduct novel research in the U.S., Denmark, and Germany, both at sea and in the lab. The scientists will also teach middle school students about the role of microbes in the carbon cycle and pressure effects on life in the ocean. The project will provide internships for high school students, focusing on first-generation students who would like to go to college. This work may aid in future efforts to identify enzymes that function well under high pressure.</p>
<p>Heterotrophic microbes (e.g., bacteria and archaea) are found throughout the ocean. Their biogeochemical functions help determine the rates and locations at which carbon and nutrients are regenerated, as well as the extent to which organic matter is preserved. Although research has shown that pressure profoundly affects the activities of marine microbes, most investigations of microbial communities of the deep sea are conducted at atmospheric pressure, due to the limited availability of specialized equipment. In collaboration with the Danish Center for Hadal Research at the University of Southern Denmark, this study will identify the effects of pressure on microbial communities and their extracellular enzymes of pressures characteristic of bathy- and abyssopelagic depths. At sea and in the lab, the scientific team will compare the effects of depressurization on the activities of enzymes produced by microbial communities of the deep ocean, as well as the effects of high pressure on surface-water derived enzymes and communities. Fieldwork will take place in Danish coastal waters, well as in the open North Atlantic and Pacific Oceans. Using pressurization systems and in situ incubations, this study will measure hydrolysis rates of peptides and polysaccharides, two of the major classes of marine organic matter. Project activities will also focus on developing the means to measure enzyme activities in situ in the deep ocean. In collaboration with colleagues from the Max Planck Institute for Marine Microbiology in Germany, this proect will additionally investigate whether pressure affects the selfish uptake of polysaccharides. These studies will provide new insight into understudied but key factors that help determine the fate of organic matter in the deep ocean.</p>
<p>This project is funded by the Biological and Chemical Oceanography Programs.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Pressure effects on microbes
largerWorkCitation
project
eng; USA
biota
oceans
Northern Izu–Ogasawara Trench
-60.05342
141.8606
34.0126
55.975
2023-09-01
2024-05-28
Western North Atlantic, hadal depths of the Pacific
0
BCO-DMO catalogue of parameters from Cell counts exhibiting 'Selfish' uptake in the Western North Atlantic, in Danish coastal seawater, and abyssopelagic waters off the eastern coast of Japan under varying hydrostatic pressures, 2023-2024
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/963597.rdf
Name: deployment
Units: unitless
Description: <p>Deployment name: AE2413 (Western North Atlantic); Helsingor - Sept 2023 (Helsingor, DK); or UM20231010 (Eastern Coast of Japan)</p>
http://lod.bco-dmo.org/id/dataset-parameter/963598.rdf
Name: station
Units: unitless
Description: <p>Station number 24, 25, 26 (AE2413); Helsingor, DK; or 7 (UM20231010)</p>
http://lod.bco-dmo.org/id/dataset-parameter/963599.rdf
Name: latitude_n
Units: Decimal degrees
Description: <p>Latitude of sampling site, positive is North</p>
http://lod.bco-dmo.org/id/dataset-parameter/963600.rdf
Name: longitude_e
Units: Decimal degrees
Description: <p>Longitude of sampling site, positive is East</p>
http://lod.bco-dmo.org/id/dataset-parameter/963601.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Datetime of sample collection (where time is available) in ISO format, UTC</p>
http://lod.bco-dmo.org/id/dataset-parameter/963602.rdf
Name: date
Units: unitless
Description: <p>Date of sample collection, based on local time</p>
http://lod.bco-dmo.org/id/dataset-parameter/963603.rdf
Name: time_local
Units: unitless
Description: <p>Time of sample collection (local time)</p>
http://lod.bco-dmo.org/id/dataset-parameter/963604.rdf
Name: cast_number
Units: unitless
Description: <p>Single collection cast identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/963605.rdf
Name: depth_description
Units: unitless
Description: <p>Water mass being sampled: Surface, Deep, DMC (Deep Chlorophyll Maximum), 2000 m</p>
http://lod.bco-dmo.org/id/dataset-parameter/963606.rdf
Name: depth_actual
Units: m
Description: <p>Depth of sample collected. Depth of in-situ syringe sampler (ISS) deployment</p>
http://lod.bco-dmo.org/id/dataset-parameter/963607.rdf
Name: insitu_temp
Units: degrees Celsius
Description: <p>Temperature of the samples in-situ</p>
http://lod.bco-dmo.org/id/dataset-parameter/963608.rdf
Name: sample_type
Units: unitless
Description: <p>The type of sample, whether it was incubated using water from the bulk water column (bulk), sediments, or amended</p>
http://lod.bco-dmo.org/id/dataset-parameter/963609.rdf
Name: incubation_type
Units: unitless
Description: <p>The type of pressure applied during incubation (using a pressure vessel or by in-situ syringe system)</p>
http://lod.bco-dmo.org/id/dataset-parameter/963610.rdf
Name: incubation_pressure
Units: MPa
Description: <p>Amount of pressure applied during incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/963611.rdf
Name: incubation_temp
Units: degrees Celsius
Description: <p>Temperature the samples were incubated at for enzyme activity measurements</p>
http://lod.bco-dmo.org/id/dataset-parameter/963612.rdf
Name: unamended_amended
Units: unitless
Description: <p>Whether the sample was amended (A) with Thalassiosira weisflogii or unamended (U)</p>
http://lod.bco-dmo.org/id/dataset-parameter/963613.rdf
Name: substrate
Units: unitless
Description: <p>Polysaccharide used for incubation: ara = arabinogalactan, chn = chondroitin sulfate, fuc = fucoidan, lam = laminarin, man = mannan, pul = pullulan, xyl = xylan, muc = mucin</p>
http://lod.bco-dmo.org/id/dataset-parameter/963614.rdf
Name: replicate
Units: unitless
Description: <p>The replicate sample and incubation number (1, or 2)</p>
http://lod.bco-dmo.org/id/dataset-parameter/963615.rdf
Name: timepoint
Units: unitless
Description: <p>The timepoint number sampled for each incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/963616.rdf
Name: timepoint_days
Units: days
Description: <p>The amount of time that has elapsed at each timepoint</p>
http://lod.bco-dmo.org/id/dataset-parameter/963617.rdf
Name: fields_of_view
Units: unitless
Description: <p>The number of images used for cell counts</p>
http://lod.bco-dmo.org/id/dataset-parameter/963618.rdf
Name: SB_Abundance
Units: Cells mL-1
Description: <p>Number of cells detected exhibiting ‘selfish’ uptake</p>
http://lod.bco-dmo.org/id/dataset-parameter/963619.rdf
Name: Total_Abundance
Units: Cells mL-1
Description: <p>Total cellular abundance</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
42988
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/f38de1dd-e53e-4860-9213-56aaf3f5e639/content
download
https://doi.org/10.26008/1912/bco-dmo.963393.1
download
onLine
dataset
<p>Water for incubation and filtration was collected via Niskin bottles mounted on a rosette, equipped with a CTD for Western North Atlantic, Danish Coastal Seawater, and Abyssopelagic Waters off the Eastern Coast of Japan.&nbsp;Additionally, in the Western North Atlantic, water was collected via an in-situ syringe-based sampler and incubator developed by the Danish Center for Hadal Research. &nbsp;</p>
<p>Niskin-collected water was used to measure cell counts and investigate selfish polysaccharide uptake under near-surface (0.1 MPa), or bathy- and abyssopelagic hydrostatic pressures (20, 40, 42 and 52 MPa). Water at each station was dispensed into smaller glass Duran bottles that were cleaned and pre-rinsed three times with water from the Niskin prior to dispensing. To 350 mL of bulk seawater or 100 mL of autoclaved seawater, substrate was added at 3.5 μM monomer-equivalent concentrations, except for fucoidan, which was added at 5 μM concentrations (a higher concentration was necessary for sufficient fluorescence signal). From these bottles, nine 6 mL Exetainer vials were filled with seawater for each substrate, replicate, and time point, and four 6 mL Exetainer vials were filled with autoclaved seawater to serve as a killed control. Sets of vials were then pressurized to either 0.1, 20, or 40 MPa in separate pressure vessels for each substrate and time point and stored in the dark at 4ºC for 0, 2, or 5 days.</p>
<p>When collected via an in-situ syringe sampler (ISS), glass syringes fitted to the sampler were preloaded with individual polysaccharide substrates to 3.5 μM monomer-equivalent concentrations or preloaded with autoclaved seawater to serve as a negative control (blank). &nbsp;Once lowered to the desired depth of 2000, 4000, 4200, or 5200 m (depending on station depth), half of the syringes were triggered so that they drew in surrounding water that mixed with the preloaded substrate or autoclaved seawater. &nbsp;The ISS was held at depth for approximately 24 hours of incubation. &nbsp;While still at depth, shortly prior to retrieval, the second half of the syringes were triggered to draw in the surrounding water. These syringes served to measure the quantity of selfish uptake occurring briefly at depth, and then during the upcast.&nbsp;</p>
<p>After sample incubation, approximately 25 mL of incubated sample was filtered through a 25 mm 0.2 µM nucleopore filter for molecular analysis, placed in cryovials, and promptly frozen at -80C.</p>
<p>Another approximately 25 mL of incubated sample was incubated with fixative for two hours, then filtered through a 25 mm 0.2 µM nucleopore filter. &nbsp;After filtration, the filters were dried in a laboratory hood, then each filter was labeled along the outer edge, placed in a Petri dish, and stored at -80C for total cell counts, and quantification of 'selfish' uptake.</p>
<p>Polysaccharide used for incubation:</p>
<ul>
<li>ara = arabinogalactan</li>
<li>chn = chondroitin sulfate</li>
<li>fuc = fucoidan</li>
<li>lam = laminarin</li>
<li>man = mannan</li>
<li>pul = pullulan</li>
<li>xyl = xylan</li>
<li>muc = mucin</li>
</ul>
Specified by the Principal Investigator(s)
<p>Image analysis was performed with ACMETOOL (http://www.technobiology.ch&nbsp;and Max Planck Institute for marine microbiology, Bremen, version 3) and Zen software package&nbsp;(Carl Zeiss).&nbsp;</p>
Specified by the Principal Investigator(s)
- Imported "20250410_BCODMO_Detection and Quantification of Cells Exhibiting 'Selfish' Uptake combined data_csv.csv" into the BCO-DMO system
- Normalized the capital letters in the "Surface" value for the "depth" parameter
- Replaced all cruise names starting with "Helsingor - Sept" to "Helsingor - Sept 2023", so they are all the same and reflect the cruise name given in the parameter descriptions
- Replaced ø → o for system requirements
- Converted date to YYYY-MM-DD
- Created ISO formatted datetime field in UTC
- Reversed the lat and lon for "AE2413", as they were reversed
- Renamed fields for clarity, consistency, and to comply with BCO-DMO naming requirements
- Exported file as "963393_v1_selfish_bacteria_cell_counts.csv"
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Zeiss LSM780 with Airyscan (Carl Zeiss)
Zeiss LSM780 with Airyscan (Carl Zeiss)
PI Supplied Instrument Name: Zeiss LSM780 with Airyscan (Carl Zeiss) PI Supplied Instrument Description:Zeiss LSM780 with Airyscan (Carl Zeiss) using a 405 nm, a 488 nm, and a 561 nm laser with detection windows of 420–480 nm, 500–550 nm, and LP 605 nm, respectively. Instrument Name: Confocal Laser Scanning Microscope Instrument Short Name: Instrument Description: A laser scanning confocal microscope is a type of confocal microscope that obtains high-resolution optical images with depth selectivity, in which a laser beam passes through a light source aperture and then is focused by an objective lens into a small (ideally diffraction-limited) focal volume within or on the surface of a specimen.
The confocal microscope uses fluorescence optics. 'Confocal' means that the image is obtained from the focal plane only, any noise resulting from sample thickness being removed optically. 'Laser scanning' means the images are acquired point by point under localized laser excitation rather than full sample illumination, as in conventional widefield microscopy.
A rosette, equipped with a CTD
A rosette, equipped with a CTD
PI Supplied Instrument Name: A rosette, equipped with a CTD PI Supplied Instrument Description:Water for incubation and filtration was collected via Niskin bottles mounted on a rosette, equipped with a CTD for Western North Atlantic, Danish Coastal Seawater, and Abyssopelagic Waters off the Eastern Coast of Japan. Instrument Name: CTD - profiler Instrument Short Name: Instrument Description: The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.
This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
Zeiss AxioImager.Z2 microscope stand, Carl Zeiss - Fully automated epifluorescence microscope
Zeiss AxioImager.Z2 microscope stand, Carl Zeiss - Fully automated epifluorescence microscope
PI Supplied Instrument Name: Zeiss AxioImager.Z2 microscope stand, Carl Zeiss - Fully automated epifluorescence microscope PI Supplied Instrument Description:Zeiss AxioImager.Z2 microscope stand, Carl Zeiss - Fully automated epifluorescence microscope Instrument Name: Fluorescence Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/
In-situ syringe-based sampler and incubator - developed by the Danish Center for Hadal Research
In-situ syringe-based sampler and incubator - developed by the Danish Center for Hadal Research
PI Supplied Instrument Name: In-situ syringe-based sampler and incubator - developed by the Danish Center for Hadal Research PI Supplied Instrument Description:Additonally, in the Western North Atlantic, water was collected via an in-situ syringe-based sampler and incubator developed by the Danish Center for Hadal Research. Instrument Name: In-situ incubator Instrument Short Name:in-situ incubator Instrument Description: A device on a ship or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/82/
Niskin bottles mounted on a rosette, equipped with a CTD
Niskin bottles mounted on a rosette, equipped with a CTD
PI Supplied Instrument Name: Niskin bottles mounted on a rosette, equipped with a CTD PI Supplied Instrument Description:Water for incubation and filtration was collected via Niskin bottles mounted on a rosette, equipped with a CTD for Western North Atlantic, Danish Coastal Seawater, and Abyssopelagic Waters off the Eastern Coast of Japan. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: AE2413
AE2413
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
AE2413
John Paul Balmonte
University of Southern Denmark
Cruise: UM20231010
UM20231010
Community Standard Description
International Council for the Exploration of the Sea
Umitaka-Maru
vessel
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
Community Standard Description
International Council for the Exploration of the Sea
Umitaka-Maru
vessel