http://lod.bco-dmo.org/id/dataset/963422
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-06-02
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Survival of larval Strongylocentrotus purpuratus under ocean warming and food availability scenarios
2025-06-09
publication
2025-06-09
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-06-09
publication
https://doi.org/10.26008/1912/bco-dmo.963422.1
Daniel K. Okamoto
Florida State University
principalInvestigator
Laura Rogers-Bennett
University of California - Davis: Bodega Marine Laboratory
principalInvestigator
Sam E. Karelitz
Florida State University
principalInvestigator
Rachel D. Simons
University of California-Santa Barbara
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Munstermann, M. J., Karelitz, S. E., Ferraro, R., Rogers-Bennett, L., Simons, R. D., Okamoto, D. K. (2025) Survival of larval Strongylocentrotus purpuratus under ocean warming and food availability scenarios. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-06-09 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.963422.1 [access date]
Larval Survival-Purple urchin Dataset Description: Methods and Sampling: <p>Trends in survival, morphometric patterns, and time to metamorphic competency across temperature and food availability treatments were measured in <em>S. purpuratus</em> larvae. Larvae were spawned from adult purple urchins collected in Santa Barbara, CA. Effects of temperature and food availability were tested using a 6 x 4 factorial experimental design with six temperature treatments (10, 12, 14, 16, 18, 20°C) and four concentrations of <em>Rhodomonas sp.</em> microalgae (500, 2500, 5000, 10,000 cells mL<sup>-1</sup>) for a total of 24 treatment combinations. Food treatments represented algal concentrations from starvation to <em>ad libitum</em> conditions.&nbsp;Temperature and food treatments were based on historical observations of temperature trends and chlorophyll abundance.&nbsp;</p>
<div>Forty <em>S. purpuratus</em> were collected from kelp forests between 2.2 – 8.8 meters from Carpinteria Reef in the Santa Barbara Channel, California in June 2022. Before spawning, individuals were held at 13°C for 4 weeks at UC Davis Bodega Marine Laboratory and were fed 20 grams of <em>Urchinomics Inc.</em> kelp-based pelleted food every other day.&nbsp;</div>
<div>
<p>A homogeneous pool of fertilized embryos were assigned to temperature treatments. Spawning was induced by injecting of 1–2 mL of 0.5 M KCl. Female urchins were placed upside down over 250 mL beakers filled with filtered to 0.1 µm seawater (FSW) at ambient temperature. Eggs were allowed to collect at the bottom of beakers. Sperm was collected dry from the aboral surface and placed on ice in 2.0 mL microcentrifuge tubes until use. Gamete quality was visually inspected with eggs assessed for shape, color, and uniform size and sperm for motility. Eggs from four females were pooled and rinsed before being fertilized by sperm from four males. Approximately equal proportions of gametes from each individual within each sex were represented.&nbsp; Successful fertilization was determined by 95% of eggs having a fertilization envelope with no polyspermy. Fertilized eggs were rinsed three times with FSW to remove excess sperm to prevent polyspermy. Approximately 2800 embryos (concentration of ~5.5 embryos mL<sup>-1</sup>) were placed in each 500 mL glass bottles filled with FSW and placed on roller tables to suspend larvae (Karelitz et al. 2020).</p>
<p>Roller agitation tables were constructed to provide water movement for larval sea urchin cultures. Rollers on free moving axles kept larval culture bottles rotating to prevent larvae from settling to the bottom.&nbsp; Bottles were partially submerged in water baths heated or chilled using Heater/Chillers (TECO US Inc. TK-500) to maintain target temperatures. Embryos were initially stocked in bottles with ambient temperature (16°C) FSW to minimize temperature shock but immediately placed into water baths to allow embryos to acclimate to target temperatures. Each of the 24 temperature x food treatments had four replicate bottles for a total of 96 bottles. Larvae were fed daily starting at 4 days post-fertilization (when larvae were observed to be competent to feed) and water changes and feeding were conducted every 2 days.</p>
<p>Larval sampling was conducted every two days to estimate changes in larval density, morphometry, and competency, in conjunction with water changes and feedings. Larval densities were quantified by concentrating larval cultures to 100 mL and counting the number of larvae found in five 1 mL subsamples. Density counts continued until larvae showed signs of metamorphosis, as determined by the presence of a rudiment and exposed tube feet.</p>
</div>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2023649 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2023649
completed
Daniel K. Okamoto
Florida State University
850-644-5260
319 Stadium Drive
Tallahassee
FL
32306
United States
dkokamoto@berkeley.edu
pointOfContact
Laura Rogers-Bennett
University of California - Davis: Bodega Marine Laboratory
707-227-7900
P.O. Box 247 2099 Westshore Road
Bodega Bay
CA
94923
USA
rogersbennett@ucdavis.edu
pointOfContact
Sam E. Karelitz
Florida State University
skarelitz@berkeley.edu
pointOfContact
Rachel D. Simons
University of California-Santa Barbara
805-893-7350
Earth Research Institute University of California, Santa Barbara
Santa Barbara
CA
93106-3060
United States
simons@eri.ucsb.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
date
temp
food
rep
count
count_value
avg_count
volume
avg_bottle
sample_day
dpf
Heater/Chillers (TECO US Inc. TK-500)
Water baths heated or chilled to maintain target temperature
Nikon dissection scope
theme
None, User defined
date
water temperature at measurement depth
treatment
replicate
count
mean
volume
days
featureType
BCO-DMO Standard Parameters
Aquarium chiller
circulating water bath
Microscope - Optical
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: The effects of marine heatwaves on reproduction, larval transport and recruitment in sea urchin metapopulations
https://osprey.bco-dmo.org/project/818918
Collaborative Research: The effects of marine heatwaves on reproduction, larval transport and recruitment in sea urchin metapopulations
<p><em>NSF Award Abstract:</em><br />
Rapid and extreme warming events such as El Niño and marine heatwaves have had ecological and economic impacts on nearshore marine ecosystems. These impacts include reductions in biomass and collapses in commercial fisheries. For many species, population booms and busts are controlled by shifts in reproduction and juvenile dispersal related to warmer temperatures and ocean circulation. However, how population fluctuations are shaped by interacting processes that control adult reproduction and larval survival remains unclear. Marine heatwaves often accompany major disruptions in ocean circulation, which can affect survival and the distribution of species that produce free-floating, planktonic larvae. As a result, species can be impacted directly by temperature effects on organismal reproduction and survival, and indirectly by shifts in ocean circulation that affect larval success. This project is examining how the joint effects of temperature and ocean circulation are controlling populations of purple sea urchins (Strongylocentrotus purpuratus). To address project objectives, the team is developing oceanographic models to predict dispersal of planktonic larvae in combination with controlled experiments on adult reproductive success. This project is advancing the understanding of how ecologically important species respond to ocean temperature and circulation, which are forecast to shift under future climate change scenarios. Broader impacts of the project include training of students and post-docs in STEM and educational outreach. Curriculum development and implementation is occurring in collaboration with existing K-12 outreach programs that focus on underserved communities and under-represented groups. The goal is to empower the next generation of scientists to use integrative approaches to predict ecological consequences of climate change.</p>
<p>Purple sea urchins are an ideal species for studying the coupled impacts of warming and ocean circulation on recruitment and survival given a wealth of ecological and organismal data. The species has a mapped genome, can be transported large distances as larvae by ocean currents, and larval abundances in California exhibit orders of magnitude variation with heatwaves and El Niño fluctuations. To quantify the processes that shape spatial and temporal variability in larval supply, researchers are applying a novel combination of biophysical modeling, experiments and statistical modeling of long-term, high-resolution data on larval settlement across the Southern California Bight (SCB). Research module 1 is quantifying spatial and temporal patterns of larval transport using a 3D-biophysical model of the SCB. The model is testing how interactions among historical changes in ocean circulation and temperature, larval life history, and larval behavioral traits affect variation in larval supply in space and time. Research module 2 is focused on how temperature could affect spatial and temporal variation in egg production. Experiments are characterizing reproductive thermal performance curves and quantifying how these vary among populations and organismal history. A novel assay is assessing epigenetic regulation of gene expression associated with performance curves. Finally, Module 3 will integrate mechanistic models from Modules 1 and 2 to statistically assess their ability to explain spatial and temporal trends in a nearly three-decade dataset of larval settlement from six sites in the SCB. This is one of the first studies that integrates models of larval transport, reproductive performance and settlement data to empirically test how physical and biological processes affect local recruitment patterns in complex marine meta-populations.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Urchin metapopulations
largerWorkCitation
project
eng; USA
biota
oceans
-119.72957
-119.718326
34.3940708
34.395849
2022-08-29
2022-10-29
Coastal California Waters from San Diego through Mendocino Counties
0
BCO-DMO catalogue of parameters from Survival of larval Strongylocentrotus purpuratus under ocean warming and food availability scenarios
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/963925.rdf
Name: date
Units: unitless
Description: <p>Date sample density was counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/963926.rdf
Name: temp
Units: degrees Celsius
Description: <p>Experimental temperature treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/963927.rdf
Name: food
Units: cells per mL
Description: <p>Experimental food concentration of Rhodomonas sp. micro algae administered to each replicate bottle</p>
http://lod.bco-dmo.org/id/dataset-parameter/963928.rdf
Name: rep
Units: unitless
Description: <p>Replicate bottle (1-4), each treatment combination had four replicate bottles</p>
http://lod.bco-dmo.org/id/dataset-parameter/963929.rdf
Name: count
Units: unitless
Description: <p>Indicates each of the five 1 mL subsamples taken from each replicate bottle to determine overall larval density in bottle</p>
http://lod.bco-dmo.org/id/dataset-parameter/963930.rdf
Name: count_value
Units: unitless
Description: <p>Indicates the number of larvae counted in each of the respective subsamples</p>
http://lod.bco-dmo.org/id/dataset-parameter/963931.rdf
Name: avg_count
Units: unitless
Description: <p>Average number of larvae across the five 1mL subsamples from one replicate bottle</p>
http://lod.bco-dmo.org/id/dataset-parameter/963932.rdf
Name: volume
Units: mL
Description: <p>Volume in which each of the five 1mL subsamples were taken from</p>
http://lod.bco-dmo.org/id/dataset-parameter/963933.rdf
Name: avg_bottle
Units: unitless
Description: <p>Estimated number of larvae in each replicate bottle across temperature and food treatments</p>
http://lod.bco-dmo.org/id/dataset-parameter/963934.rdf
Name: sample_day
Units: days
Description: <p>Indicates the sampling day (i.e., the first day larvae were sampled is indicated with a '1')</p>
http://lod.bco-dmo.org/id/dataset-parameter/963935.rdf
Name: dpf
Units: days
Description: <p>Days post fertilization</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
455591
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/f1aa7225-90b0-4b38-b73d-5f8cd9c87660/content
download
https://doi.org/10.26008/1912/bco-dmo.963422.1
download
onLine
dataset
<p>Trends in survival, morphometric patterns, and time to metamorphic competency across temperature and food availability treatments were measured in <em>S. purpuratus</em> larvae. Larvae were spawned from adult purple urchins collected in Santa Barbara, CA. Effects of temperature and food availability were tested using a 6 x 4 factorial experimental design with six temperature treatments (10, 12, 14, 16, 18, 20°C) and four concentrations of <em>Rhodomonas sp.</em> microalgae (500, 2500, 5000, 10,000 cells mL<sup>-1</sup>) for a total of 24 treatment combinations. Food treatments represented algal concentrations from starvation to <em>ad libitum</em> conditions.&nbsp;Temperature and food treatments were based on historical observations of temperature trends and chlorophyll abundance.&nbsp;</p>
<div>Forty <em>S. purpuratus</em> were collected from kelp forests between 2.2 – 8.8 meters from Carpinteria Reef in the Santa Barbara Channel, California in June 2022. Before spawning, individuals were held at 13°C for 4 weeks at UC Davis Bodega Marine Laboratory and were fed 20 grams of <em>Urchinomics Inc.</em> kelp-based pelleted food every other day.&nbsp;</div>
<div>
<p>A homogeneous pool of fertilized embryos were assigned to temperature treatments. Spawning was induced by injecting of 1–2 mL of 0.5 M KCl. Female urchins were placed upside down over 250 mL beakers filled with filtered to 0.1 µm seawater (FSW) at ambient temperature. Eggs were allowed to collect at the bottom of beakers. Sperm was collected dry from the aboral surface and placed on ice in 2.0 mL microcentrifuge tubes until use. Gamete quality was visually inspected with eggs assessed for shape, color, and uniform size and sperm for motility. Eggs from four females were pooled and rinsed before being fertilized by sperm from four males. Approximately equal proportions of gametes from each individual within each sex were represented.&nbsp; Successful fertilization was determined by 95% of eggs having a fertilization envelope with no polyspermy. Fertilized eggs were rinsed three times with FSW to remove excess sperm to prevent polyspermy. Approximately 2800 embryos (concentration of ~5.5 embryos mL<sup>-1</sup>) were placed in each 500 mL glass bottles filled with FSW and placed on roller tables to suspend larvae (Karelitz et al. 2020).</p>
<p>Roller agitation tables were constructed to provide water movement for larval sea urchin cultures. Rollers on free moving axles kept larval culture bottles rotating to prevent larvae from settling to the bottom.&nbsp; Bottles were partially submerged in water baths heated or chilled using Heater/Chillers (TECO US Inc. TK-500) to maintain target temperatures. Embryos were initially stocked in bottles with ambient temperature (16°C) FSW to minimize temperature shock but immediately placed into water baths to allow embryos to acclimate to target temperatures. Each of the 24 temperature x food treatments had four replicate bottles for a total of 96 bottles. Larvae were fed daily starting at 4 days post-fertilization (when larvae were observed to be competent to feed) and water changes and feeding were conducted every 2 days.</p>
<p>Larval sampling was conducted every two days to estimate changes in larval density, morphometry, and competency, in conjunction with water changes and feedings. Larval densities were quantified by concentrating larval cultures to 100 mL and counting the number of larvae found in five 1 mL subsamples. Density counts continued until larvae showed signs of metamorphosis, as determined by the presence of a rudiment and exposed tube feet.</p>
</div>
Specified by the Principal Investigator(s)
- Imported "roller_density_counts.csv" into BCO-DMO system
- Export file as "963422_v1_roller_density_counts.csv"
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Heater/Chillers (TECO US Inc. TK-500)
Heater/Chillers (TECO US Inc. TK-500)
PI Supplied Instrument Name: Heater/Chillers (TECO US Inc. TK-500) PI Supplied Instrument Description:Bottles were partially submerged in water baths heated or chilled using Heater/Chillers (TECO US Inc. TK-500) to maintain target temperatures. Instrument Name: Aquarium chiller Instrument Short Name:Aquarium chiller Instrument Description: Immersible or in-line liquid cooling device, usually with temperature control.
Water baths heated or chilled to maintain target temperature
Water baths heated or chilled to maintain target temperature
PI Supplied Instrument Name: Water baths heated or chilled to maintain target temperature PI Supplied Instrument Description:Roller agitation tables were constructed to provide water movement for larval sea urchin cultures. Rollers on free moving axles kept larval culture bottles rotating to prevent larvae from settling to the bottom. Bottles were partially submerged in water baths heated or chilled using Heater/Chillers (TECO US Inc. TK-500) to maintain target temperatures. Instrument Name: circulating water bath Instrument Short Name: Instrument Description: A device designed to regulate the temperature of a vessel by bathing it in water held at the desired temperature. [Definition Source: NCI]
Nikon dissection scope
Nikon dissection scope
PI Supplied Instrument Name: Nikon dissection scope PI Supplied Instrument Description:Larval densities were quantified by concentrating larval cultures to 100 mL and counting the number of larvae found in five 1 mL subsamples. A Nikon dissection scope was used to count larvae. Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/