http://lod.bco-dmo.org/id/dataset/964162
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-06-09
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Performance of coral and sea anemone early life stages under normoxic and hypoxic conditions from lab and field experiments performed in Pennsylvania (USA) and Bermuda in 2023-2024
2025-06-13
publication
2025-06-13
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-06-20
publication
https://doi.org/10.26008/1912/bco-dmo.964162.1
Katie Barott
University of Pennsylvania
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Glass, B., Barott, K. (2025) Performance of coral and sea anemone early life stages under normoxic and hypoxic conditions from lab and field experiments performed in Pennsylvania (USA) and Bermuda in 2023-2024. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-06-13 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.964162.1 [access date]
Performance of coral and sea anemone early life stages under normoxic and hypoxic conditions Dataset Description: Methods and Sampling: <p><strong>Adult culture and spawning</strong></p>
<p>Adult&nbsp;<em>Nematostella vectensis</em>&nbsp;sea anemones were collected from a salt marsh in Brigantine, New Jersey in the fall of 2020, and spawning was induced. Larvae (n = 1 cohort with mixed parentage) were then cultured to the planula stage (3 d post-fertilization) for experimentation. An aquarium population of adult&nbsp;<em>Galaxea fascicularis</em>&nbsp;colonies (n = 9 females, 10 males) spawned during August 2023 at Carnegie Science (Baltimore, MD, USA), yielding a cohort of planulae (n = 1 cohort with mixed parentage) that were used in experiments within 48 h. Adult&nbsp;P<em>orites astreoides</em>&nbsp;colones (n = 20) were collected in Bermuda (32°22′13″N, 64°44′27″W) during July 2023. Brooded planulae (n = 4 cohorts) were collected&nbsp;and maintained in culture for ~ 48 h.</p>
<p>At the time of use in experimentation, larvae from all 3 species were in the planula stage and would become competent to settle within 72–96 h (Hand and Uhlinger&nbsp;1992; Goodbody-Gringley et al.&nbsp;2018; Wei et al.&nbsp;2023). Artificial seawater was used for culturing and experimentation for&nbsp;<em>N. vectensis</em>&nbsp;and&nbsp;<em>G. fascicularis</em>, while flow-through, natural seawater facilities at the Bermuda Institute for Ocean Science were used for&nbsp;<em>P. astreoides</em>.</p>
<p><strong>Dissoved Oxygen Treatments</strong></p>
<p>Stage-matched, swimming planulae of <em>N. vectensis</em>, <em>G. fascicularis</em>, and <em>P. astreoides</em>&nbsp;(N = 1,200–2,400 larvae species-1) were divided into six replicate groups (three normoxia/control and three hypoxia) and exposed to 6 h of normoxia (dissolved oxygen (DO) = 6.8–8.69 mg L-1) or severe hypoxia (DO = 1.58–1.8 mg L-1; seawater deoxygenated using N2 gas) inside sealed glass jars (500 mL) overnight from 21:00 h to 03:00 h the following day. The jars were placed at the ambient culture temperature for each species (N. vectensis: 18°C; G. fascicularis: 27°C; P. astreoides: 28°C). At the end of the treatment period, the jars were uncapped and groups of 20–30 larvae (N = 60–90 larvae treatment-1 species-1) were transferred to 1.5 mL tubes without seawater for storage at -80°C until processing</p>
<p><strong>Measurements</strong></p>
<p>Detailed methods can be found in the associated publication (Glass and Barott&nbsp;2025).</p>
<ul>
<li>Ash-free dry weight (AFDW; i.e., organic biomass) of larvae and juveniles was quantified from burned, dried tissue.</li>
<li>Chlorophyll content within algal endosymbionts of larvae and juveniles was quantified via spectroscopy.</li>
<li>Heat tolerance of <em>P. astreoides</em> larval symbionts was quantified via changes in photochemical yield (collected with a pulse-amplitude-modulation (PAM) fluorometer) over time at 36°C.</li>
<li>Heat tolerance of larvae was quantified via visual observation of survival over time at 36°C. Photochemical yield of <em>P. astreoides</em> larvae and juveniles as quantified using a pulse-amplitude-modulation (PAM) fluorometer.</li>
<li>Metabolic rates of larvae and juveniles was quantified via respirometry.</li>
<li>Protein content larvae and juveniles was quantified via Bradford assays.</li>
<li>Larval settlement rates were quantified via brightfield microscopy.</li>
<li>Larval and juvenile sizes were quantified via brightfield microscopy.</li>
<li>Larval swimming behavior immediately following the oxygen treatments was quantified via imaging using iPhone 14 (Apple).</li>
<li>Larval and juvenile symbiont density was quantified via flow cytometry.&nbsp;</li>
</ul>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2237658 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2237658
completed
Katie Barott
University of Pennsylvania
215-746-6911
433 South University Avenue Leidy Laboratories 326
Philadelphia
PA
19104
USA
kbarott@sas.upenn.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Data_filename
Species
Treatment
Cohort
Group
Life_stage
Hours_post_treatment
AFDW_ug_mm
Chlorophyll_pg_symbiont
Hours_at_36_C
Dark_adapted_Fv_Fm_1
Larvae_surviving
Total_larvae
Proportion_surviving
Percent_surviving
Photo_pmol_O2_min_ug_AFDW
Protein_ug_mm
Resp_pmol_O2_min_ug_AFDW
Settlement_rate_percent
Size_mm
Larvae_in_water_column_percent
Symbiont_density_cells_ug_AFDW
Time_in_treatment_hours
Temperature_C
Salinity_ppt
Dissolved_oxygen_mg_L
iPhone 14 (Apple)
Cytek biosciences guava flow cytometer
Pulse-amplitude-modulation (PAM) fluorometer
microscope (AmScope)
YSI ProSolo handheld probe
SensorDish Reader from Precision Sensing
spectrophotometer (Agilent)
theme
None, User defined
file_name
species scientific name (binomial)
treatment
sample description
stage
duration
dry_wgt
chlorophyll a
Fv to Fm ratio
count
ratio
photosynthetic rate
protein concentration
respiration rate
fecundity
length
water temperature at measurement depth
salinity
dissolved Oxygen
featureType
BCO-DMO Standard Parameters
Camera
Flow Cytometer
Fluorometer
Microscope - Optical
Oxygen Sensor
Respirometer
Spectrophotometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
CAREER: Helping or hindering? Determining the influence of repetitive marine heatwaves on acclimatization of reef-building corals across biological scales
https://osprey.bco-dmo.org/project/925169
CAREER: Helping or hindering? Determining the influence of repetitive marine heatwaves on acclimatization of reef-building corals across biological scales
<p><em>NSF Award Abstract:</em></p>
<p><span style="color:rgb(27, 27, 27); font-family:verdana; font-size:11.312px">Ocean warming driven by climate change has led to staggering losses of coral on reefs worldwide and is now among the most pressing of stressors threatening the survival of coral reef ecosystems today. As marine heatwaves associated with ocean warming become increasingly frequent, it is urgent to understand if and how reef-building corals will be able to respond to these repeat stress events and thus survive in a rapidly warming ocean. To address this problem, this project is investigating how corals on the reef respond to recurring marine heatwaves in order to identify if repeat exposure to heat stress promotes coral tolerance of higher temperatures via acclimatization or instead leads to the accumulation of stress and thus reduced performance and survival following future stress. The results of this study are critical for understanding how the current generation of corals will respond to increasingly warmer oceans, and whether acclimatization will buy enough individuals sufficient time for adaptation to occur and promote coral persistence into the future. In addition, this project is training students from secondary schools through advanced postdoctoral researchers in global change biology and ecology. Specifically, the investigators are increasing access to research opportunities for undergraduate students by developing a new hands-on course-based undergraduate research experience (CURE) in Global Ocean Change Biology that will reach hundreds of students per year. Outreach efforts include creation of hands-on coral reefs and climate change activities for incoming first-generation, low-income undergraduate students and a professional development program to train middle and high-school teachers to deploy these climate change activities in their classrooms in the Philadelphia Public School District.</span></p>
<p><span style="color:rgb(27, 27, 27); font-family:verdana; font-size:11.312px">Acclimatization following exposure to sub-lethal heat stress may be an important protective mechanism for corals to survive a changing climate. However, the role of environmental memory of marine heatwaves in driving acclimatization or, conversely, stress accumulation and sensitization of reef-building corals is not well understood. This study is addressing this question using a combination of in situ and mesocosm experiments to assess the cellular, organismal, and ecological consequences of repeat heatwaves on corals with contrasting bleaching histories. Specifically, the researchers are monitoring adjacent conspecific pairs of bleaching-susceptible and bleaching-resistant individuals of two reef-building coral species in Hawaii, Montipora capitata and Porites compressa. These corals have been monitored for over 7 years through multiple bleaching events and are being used to test the hypothesis that environmental memory of marine heatwaves differentially alters coral thermal performance due to phenotypic variation in acclimatization ability within and between species. This work is identifying whether the bleaching thresholds of corals with different bleaching histories varies through time, and the consequences of these phenotypes on coral calcification, survival, and population size structure are being assessed using a combination of benthic surveys, photogrammetry, and in situ growth measurements. The influence of environmental memory of heatwaves on coral physiology is being assessed using thermal performance curves to determine how the thermal optima of respiration, photosynthesis, calcification, and host intracellular pH change (or not) over time (e.g. ambient vs. heatwave years) and if that response differs between corals with contrasting bleaching phenotypes. Finally, the contribution of algal endosymbionts to acclimatization is being evaluated by exposing corals to a range of increasing temperatures in experimental mesocosms, potentially uncovering differences in the degree of acclimatization or sensitization for host vs. symbiont traits in corals with high fidelity (P. compressa) vs. cosmopolitan (M. capitata) symbiont associations. By understanding of the phenotypic diversity in thermal performance across biological scales, this research improves predictions of coral persistence in the face of the ongoing climate crisis.</span></p>
<p><span style="color:rgb(27, 27, 27); font-family:verdana; font-size:11.312px">This project is supported by the Biological Oceanography, Integrative Ecological Physiology, and Ocean Education Programs.</span></p>
<p><span style="color:rgb(27, 27, 27); font-family:verdana; font-size:11.312px">This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</span></p>
Coral acclimatization
largerWorkCitation
project
eng; USA
biota
oceans
2023-06-01
2024-11-01
Kaneohe Bay, Oahu, Hawaii
0
BCO-DMO catalogue of parameters from Performance of coral and sea anemone early life stages under normoxic and hypoxic conditions from lab and field experiments performed in Pennsylvania (USA) and Bermuda in 2023-2024
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/965331.rdf
Name: Data_filename
Units: unitless
Description: <p>Supplemental filename, data source</p>
http://lod.bco-dmo.org/id/dataset-parameter/965332.rdf
Name: Species
Units: unitless
Description: <p>Species from which the data originate</p>
http://lod.bco-dmo.org/id/dataset-parameter/965333.rdf
Name: Treatment
Units: unitless
Description: <p>Oxygen treatment to which larvae were exposed (either normoxia or hypoxia)</p>
http://lod.bco-dmo.org/id/dataset-parameter/965334.rdf
Name: Cohort
Units: unitless
Description: <p>Cohort (i.e., group of larvae born at the same time) from which the data originate</p>
http://lod.bco-dmo.org/id/dataset-parameter/965335.rdf
Name: Group
Units: unitless
Description: <p>Experimental treatment jar (i.e., biological replicate) from which the data originate</p>
http://lod.bco-dmo.org/id/dataset-parameter/965336.rdf
Name: Life_stage
Units: unitless
Description: <p>Developmental/life history stage of animals at time of data collection (either larva or juvenile)</p>
http://lod.bco-dmo.org/id/dataset-parameter/965337.rdf
Name: Hours_post_treatment
Units: hours
Description: <p>Hours after hypoxia treatment at which time data were collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/965338.rdf
Name: AFDW_ug_mm
Units: µg/mm
Description: <p>Ash-free dry weight of animals measured in µg and normalized to size in mm</p>
http://lod.bco-dmo.org/id/dataset-parameter/965339.rdf
Name: Chlorophyll_pg_symbiont
Units: pg/cell
Description: <p>Chlorophyll content of endosymbiont cells measured in pg and normalized per symbiont cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/965340.rdf
Name: Hours_at_36_C
Units: hours
Description: <p>Hours after which larvae were placed at 36°C at which time data were collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/965341.rdf
Name: Dark_adapted_Fv_Fm_1
Units: unitless
Description: <p>Photochemical yield</p>
http://lod.bco-dmo.org/id/dataset-parameter/965342.rdf
Name: Larvae_surviving
Units: unitless
Description: <p>The number of larvae surviving at the time of data collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/965343.rdf
Name: Total_larvae
Units: unitless
Description: <p>The total number of larvae exposed to heat stress (always 8)</p>
http://lod.bco-dmo.org/id/dataset-parameter/965344.rdf
Name: Proportion_surviving
Units: unitless
Description: <p>Larvae_surviving / total_larvae</p>
http://lod.bco-dmo.org/id/dataset-parameter/965345.rdf
Name: Percent_surviving
Units: percent
Description: <p>Proportion_surviving * 100</p>
http://lod.bco-dmo.org/id/dataset-parameter/965346.rdf
Name: Photo_pmol_O2_min_ug_AFDW
Units: pmol/min/µg
Description: <p>Photosynthesis rate expressed as pmol O2 (oxygen) produced per minute per µg of ash-free dry weight</p>
http://lod.bco-dmo.org/id/dataset-parameter/965347.rdf
Name: Protein_ug_mm
Units: µg/mm
Description: <p>Cellular protein content expressed as µg protein per size (in mm)</p>
http://lod.bco-dmo.org/id/dataset-parameter/965348.rdf
Name: Resp_pmol_O2_min_ug_AFDW
Units: pmol/min/µg
Description: <p>Respiration rate expressed as pmol O2 (oxygen) consumed per minute per µg of ash-free dry weight</p>
http://lod.bco-dmo.org/id/dataset-parameter/965349.rdf
Name: Settlement_rate_percent
Units: percent
Description: <p>Percentage of larvae settled on the final day of data collection (i.e., final/maximum settlement rate)</p>
http://lod.bco-dmo.org/id/dataset-parameter/965350.rdf
Name: Size_mm
Units: mm
Description: <p>Size of larvae (length) and juveniles (polyp diameter) as measured via microscopy</p>
http://lod.bco-dmo.org/id/dataset-parameter/965351.rdf
Name: Larvae_in_water_column_percent
Units: percent
Description: <p>Percentage of larvae actively swimming in the water column immediately at the conclusions of the treatment period</p>
http://lod.bco-dmo.org/id/dataset-parameter/965352.rdf
Name: Symbiont_density_cells_ug_AFDW
Units: cells/µg
Description: <p>Number of symbiont cells within larvae or juveniles expressed as the number of cells per µg of ash-free dry weight</p>
http://lod.bco-dmo.org/id/dataset-parameter/965353.rdf
Name: Time_in_treatment_hours
Units: hours
Description: <p>Time since the beginning of the treatment period expressed in hours</p>
http://lod.bco-dmo.org/id/dataset-parameter/965354.rdf
Name: Temperature_C
Units: degrees Celsius
Description: <p>Seawater temperature as determined via a handheld probe expressed in °C</p>
http://lod.bco-dmo.org/id/dataset-parameter/965355.rdf
Name: Salinity_ppt
Units: parts per thousand (ppt)
Description: <p>Seawater salinity as determined via a handheld probe expressed in parts per thousand (ppt)</p>
http://lod.bco-dmo.org/id/dataset-parameter/965356.rdf
Name: Dissolved_oxygen_mg_L
Units: mg/L
Description: <p>Seawater dissolved oxygen concentration as determined via a handheld probe expressed in mg per L of seawater</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
261280
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/4ad8575a-0f1a-400e-b171-c8f8fc5abf59/content
download
https://doi.org/10.26008/1912/bco-dmo.964162.1
download
onLine
dataset
<p><strong>Adult culture and spawning</strong></p>
<p>Adult&nbsp;<em>Nematostella vectensis</em>&nbsp;sea anemones were collected from a salt marsh in Brigantine, New Jersey in the fall of 2020, and spawning was induced. Larvae (n = 1 cohort with mixed parentage) were then cultured to the planula stage (3 d post-fertilization) for experimentation. An aquarium population of adult&nbsp;<em>Galaxea fascicularis</em>&nbsp;colonies (n = 9 females, 10 males) spawned during August 2023 at Carnegie Science (Baltimore, MD, USA), yielding a cohort of planulae (n = 1 cohort with mixed parentage) that were used in experiments within 48 h. Adult&nbsp;P<em>orites astreoides</em>&nbsp;colones (n = 20) were collected in Bermuda (32°22′13″N, 64°44′27″W) during July 2023. Brooded planulae (n = 4 cohorts) were collected&nbsp;and maintained in culture for ~ 48 h.</p>
<p>At the time of use in experimentation, larvae from all 3 species were in the planula stage and would become competent to settle within 72–96 h (Hand and Uhlinger&nbsp;1992; Goodbody-Gringley et al.&nbsp;2018; Wei et al.&nbsp;2023). Artificial seawater was used for culturing and experimentation for&nbsp;<em>N. vectensis</em>&nbsp;and&nbsp;<em>G. fascicularis</em>, while flow-through, natural seawater facilities at the Bermuda Institute for Ocean Science were used for&nbsp;<em>P. astreoides</em>.</p>
<p><strong>Dissoved Oxygen Treatments</strong></p>
<p>Stage-matched, swimming planulae of <em>N. vectensis</em>, <em>G. fascicularis</em>, and <em>P. astreoides</em>&nbsp;(N = 1,200–2,400 larvae species-1) were divided into six replicate groups (three normoxia/control and three hypoxia) and exposed to 6 h of normoxia (dissolved oxygen (DO) = 6.8–8.69 mg L-1) or severe hypoxia (DO = 1.58–1.8 mg L-1; seawater deoxygenated using N2 gas) inside sealed glass jars (500 mL) overnight from 21:00 h to 03:00 h the following day. The jars were placed at the ambient culture temperature for each species (N. vectensis: 18°C; G. fascicularis: 27°C; P. astreoides: 28°C). At the end of the treatment period, the jars were uncapped and groups of 20–30 larvae (N = 60–90 larvae treatment-1 species-1) were transferred to 1.5 mL tubes without seawater for storage at -80°C until processing</p>
<p><strong>Measurements</strong></p>
<p>Detailed methods can be found in the associated publication (Glass and Barott&nbsp;2025).</p>
<ul>
<li>Ash-free dry weight (AFDW; i.e., organic biomass) of larvae and juveniles was quantified from burned, dried tissue.</li>
<li>Chlorophyll content within algal endosymbionts of larvae and juveniles was quantified via spectroscopy.</li>
<li>Heat tolerance of <em>P. astreoides</em> larval symbionts was quantified via changes in photochemical yield (collected with a pulse-amplitude-modulation (PAM) fluorometer) over time at 36°C.</li>
<li>Heat tolerance of larvae was quantified via visual observation of survival over time at 36°C. Photochemical yield of <em>P. astreoides</em> larvae and juveniles as quantified using a pulse-amplitude-modulation (PAM) fluorometer.</li>
<li>Metabolic rates of larvae and juveniles was quantified via respirometry.</li>
<li>Protein content larvae and juveniles was quantified via Bradford assays.</li>
<li>Larval settlement rates were quantified via brightfield microscopy.</li>
<li>Larval and juvenile sizes were quantified via brightfield microscopy.</li>
<li>Larval swimming behavior immediately following the oxygen treatments was quantified via imaging using iPhone 14 (Apple).</li>
<li>Larval and juvenile symbiont density was quantified via flow cytometry.&nbsp;</li>
</ul>
Specified by the Principal Investigator(s)
<p>Full details regarding data and statistical analyses are present in Glass and Barott (2025), Supporting Information.&nbsp;RStudio with R version 4.2.1 was used for all analyses (RStudio Team&nbsp;2020).&nbsp;All values are expressed as averages rounded to appropriate significant figures ± standard error of the mean (SEM), and all original data is attached as supplemental files and published (Glass, B., &amp; Barott, K. 2024), as referenced in the related datasets section. The code used (Glass, B., &amp; Barott, K. 2024) is listed in the related publication section.&nbsp;</p>
Specified by the Principal Investigator(s)
- Imported "AFDW_data.csv", "Chlorophyll_data.csv", "Heat_tolerance_PAM_data.csv", "Heat_tolerance_survival_data.csv", "PAM_data.csv", "Photosynthesis_data.csv", "Protein_data.csv", "Respiration_data.csv", "Settlement_data.csv", "Size_data.csv", "Swimming_behavior_data.csv", "Symbiont_density_data.csv", "Treatment_seawater_conditions_data.csv" into the BCO-DMO system
- Concatenated all datafiles with their original file names in the "Data_filename" parameter
- Exported file as "964162_v1_performance_early_life_stages.csv"
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
iPhone 14 (Apple)
iPhone 14 (Apple)
PI Supplied Instrument Name: iPhone 14 (Apple) PI Supplied Instrument Description:Larval swimming behavior immediately following the oxygen treatments was quantified via imaging using iPhone 14 (Apple). Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/
Cytek biosciences guava flow cytometer
Cytek biosciences guava flow cytometer
PI Supplied Instrument Name: Cytek biosciences guava flow cytometer PI Supplied Instrument Description:Larval and juvenile symbiont density was quantified via flow cytometry. Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
Pulse-amplitude-modulation (PAM) fluorometer
Pulse-amplitude-modulation (PAM) fluorometer
PI Supplied Instrument Name: Pulse-amplitude-modulation (PAM) fluorometer PI Supplied Instrument Description:Heat tolerance of P. astreoides larval symbionts was quantified via changes in photochemical yield (collected with a pulse-amplitude-modulation (PAM) fluorometer) over time at 36°C. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
microscope (AmScope)
microscope (AmScope)
PI Supplied Instrument Name: microscope (AmScope) PI Supplied Instrument Description:Larval settlement rates were quantified via brightfield microscopy. Larval and juvenile sizes were quantified via brightfield microscopy. Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
YSI ProSolo handheld probe
YSI ProSolo handheld probe
PI Supplied Instrument Name: YSI ProSolo handheld probe PI Supplied Instrument Description:Seawater temperature as determined via a handheld probe expressed in °C. Seawater salinity as determined via a handheld probe expressed in parts per thousand (ppt). Seawater dissolved oxygen concentration as determined via a handheld probe expressed in mg per L of seawater. Instrument Name: Oxygen Sensor Instrument Short Name:Dissolved Oxygen Sensor Instrument Description: An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed
SensorDish Reader from Precision Sensing
SensorDish Reader from Precision Sensing
PI Supplied Instrument Name: SensorDish Reader from Precision Sensing PI Supplied Instrument Description:Metabolic rates of larvae and juveniles was quantified via respirometry. Instrument Name: Respirometer Instrument Short Name: Instrument Description: A device that measures the rate of respiration by a living organism or organic system by measuring its rate of exchange of oxygen and/or carbon dioxide.
spectrophotometer (Agilent)
spectrophotometer (Agilent)
PI Supplied Instrument Name: spectrophotometer (Agilent) PI Supplied Instrument Description:Chlorophyll content within algal endosymbionts of larvae and juveniles was quantified via spectroscopy. Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/