http://lod.bco-dmo.org/id/dataset/964167
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-06-09
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Physiological measurements from sea anemones exposed to normoxic or hypoxic conditions from lab experiments performed in Philadelphia, PA, USA in 2024
2025-06-24
publication
2025-06-24
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-07-07
publication
https://doi.org/10.26008/1912/bco-dmo.964167.1
Katie Barott
University of Pennsylvania
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Glass, B., Barott, K. (2025) Physiological measurements from sea anemones exposed to normoxic or hypoxic conditions from lab experiments performed in Philadelphia, PA, USA in 2024. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-06-24 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.964167.1 [access date]
Physiological measurements from sea anemones exposed to normoxic or hypoxic conditions Dataset Description: Methods and Sampling: <p><strong>Sea Anemone Collection and Culture</strong></p>
<p>Adult&nbsp;Nematostella vectensis&nbsp;sea anemones were collected from the JC NERR near Brigantine, New Jersey, USA in 2020.&nbsp;Spawning was induced approximately twice per month using a standard method for&nbsp;<em>Nematostella vectensis</em>&nbsp;(Hand and Uhlinger&nbsp;1992; Stefanik et&nbsp;al.&nbsp;2013).&nbsp;Larval anemones were retained in culture dishes and advanced to the adult stage over 2 years, then spawned in individual cups to identify female and male animals for use in the experiment.&nbsp;</p>
<p>After seven days of acclimation, adult anemones were distributed across 6 tubs (N = 3 tubs per DO treatment: normoxia/control and hypoxia) with 15 females and 14 males in each tub (N = 87 animals per treatment). Next, spawning was induced on day 7 of acclimation to clear anemones of developing gametes (Glass, Schmitt, et&nbsp;al.&nbsp;2023; Rivera et&nbsp;al.&nbsp;2021). Total water changes were then performed for each tub, and animals were placed back in ambient culture (i.e., normoxia) for the start of the experimental period (i.e., experimental day 0).&nbsp;</p>
<p><strong>Hypoxia Treatments</strong></p>
<p>Over nights 5, 6, and 7 of the experimental period, adult anemones were exposed to either normoxia (i.e., controls; DO = 7–10 mg L−1) or hypoxia (DO = 0.5–1.5 mg L−1) for ~12 h night−1. Nitrogen gas was used to deoxygenate the water in a glass jar filled nearly to the brim (500 mL; Ball Corporation, Westminster, CO, USA) to a value of ~1.5 mg L−1&nbsp;(salinity corrected) as determined by a handheld DO probe (YSI ProSolo 626650; accuracy = ±0.02 and precision = 0.01 mg L−1; YSI, Yellow Springs, OH, USA). The anemones from 1 culture tub (N = 29 animals) were transferred with minimal culture water (containing no&nbsp;Artemia) into the jar, which was then topped off with anoxic 12 ppt ASW, capped, and covered with plastic wrap secured by a rubber band. This process was repeated for the 5 other jars, skipping the seawater deoxygenation step for the 3 normoxia jars, and all 6 jars were placed in a dark incubator at 18°C. While anemones were in the treatment jars, the (normoxic) seawater from the culture tubs was pooled and filtered (mesh size 100 μm) before being redistributed across the tubs to minimise block effects by tub and maintain water cleanliness by removing particulate waste, though this may have introduced a confounding factor and should be considered when interpreting the data.</p>
<p>After ~12 h, the jars were opened and a DO reading was quickly recorded. Anemones were then transferred back into their respective tubs with minimal water carried over from the treatment jars. Both animals exposed to hypoxia and controls were handled in the same manner.</p>
<p><strong>Measurements</strong></p>
<p>Detailed methods can be found in the associated publication (Glass et al. 2025).</p>
<ul>
<li>Dry weight of adult sea anemones was determined using a drying oven and lab balance.</li>
<li>Respiration rates of adult and larval sea anemones were determined via respirometry using a PreSens sensor dish reader system.</li>
<li>Female fecundity was determined by counting eggs imaged via brightifled microscopy.</li>
<li>Male fecundity was determined by counting sperm via flow cytometry.</li>
<li>Egg size was determined by measuring eggs in images collected via brightfield microscopy.</li>
<li>Sperm mitochondrial membrane potential was determined using the fluorescent dye JC-1 analyzed via flow cytometry.</li>
<li>Larval developmental success and settlement rates were determined via visual inspection under brightfield microscopy.</li>
</ul>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2237658 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2237658
completed
Katie Barott
University of Pennsylvania
215-746-6911
433 South University Avenue Leidy Laboratories 326
Philadelphia
PA
19104
USA
kbarott@sas.upenn.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample_name
Sample_ID
Treatment
Group
Sex
Average_dry_weight
Average_respiration
Average_eggs_dry_weight
Average_egg_volume
Development_success_percent
Average_relative_fitness
Average_sperm_M_dry_weight
Average_sperm_M_respiration
Average_sperm_red_green_ratio
Spawned_percent
drying oven
Cytek guava flow cytometer
Microscope
SensorDish Reader from Precision Sensing
lab balance
theme
None, User defined
sample identification
treatment
sample description
sex
dry_wgt
respiration rate
fecundity
featureType
BCO-DMO Standard Parameters
Drying Oven
Flow Cytometer
Microscope - Optical
Respirometer
scale or balance
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
CAREER: Helping or hindering? Determining the influence of repetitive marine heatwaves on acclimatization of reef-building corals across biological scales
https://osprey.bco-dmo.org/project/925169
CAREER: Helping or hindering? Determining the influence of repetitive marine heatwaves on acclimatization of reef-building corals across biological scales
<p><em>NSF Award Abstract:</em></p>
<p><span style="color:rgb(27, 27, 27); font-family:verdana; font-size:11.312px">Ocean warming driven by climate change has led to staggering losses of coral on reefs worldwide and is now among the most pressing of stressors threatening the survival of coral reef ecosystems today. As marine heatwaves associated with ocean warming become increasingly frequent, it is urgent to understand if and how reef-building corals will be able to respond to these repeat stress events and thus survive in a rapidly warming ocean. To address this problem, this project is investigating how corals on the reef respond to recurring marine heatwaves in order to identify if repeat exposure to heat stress promotes coral tolerance of higher temperatures via acclimatization or instead leads to the accumulation of stress and thus reduced performance and survival following future stress. The results of this study are critical for understanding how the current generation of corals will respond to increasingly warmer oceans, and whether acclimatization will buy enough individuals sufficient time for adaptation to occur and promote coral persistence into the future. In addition, this project is training students from secondary schools through advanced postdoctoral researchers in global change biology and ecology. Specifically, the investigators are increasing access to research opportunities for undergraduate students by developing a new hands-on course-based undergraduate research experience (CURE) in Global Ocean Change Biology that will reach hundreds of students per year. Outreach efforts include creation of hands-on coral reefs and climate change activities for incoming first-generation, low-income undergraduate students and a professional development program to train middle and high-school teachers to deploy these climate change activities in their classrooms in the Philadelphia Public School District.</span></p>
<p><span style="color:rgb(27, 27, 27); font-family:verdana; font-size:11.312px">Acclimatization following exposure to sub-lethal heat stress may be an important protective mechanism for corals to survive a changing climate. However, the role of environmental memory of marine heatwaves in driving acclimatization or, conversely, stress accumulation and sensitization of reef-building corals is not well understood. This study is addressing this question using a combination of in situ and mesocosm experiments to assess the cellular, organismal, and ecological consequences of repeat heatwaves on corals with contrasting bleaching histories. Specifically, the researchers are monitoring adjacent conspecific pairs of bleaching-susceptible and bleaching-resistant individuals of two reef-building coral species in Hawaii, Montipora capitata and Porites compressa. These corals have been monitored for over 7 years through multiple bleaching events and are being used to test the hypothesis that environmental memory of marine heatwaves differentially alters coral thermal performance due to phenotypic variation in acclimatization ability within and between species. This work is identifying whether the bleaching thresholds of corals with different bleaching histories varies through time, and the consequences of these phenotypes on coral calcification, survival, and population size structure are being assessed using a combination of benthic surveys, photogrammetry, and in situ growth measurements. The influence of environmental memory of heatwaves on coral physiology is being assessed using thermal performance curves to determine how the thermal optima of respiration, photosynthesis, calcification, and host intracellular pH change (or not) over time (e.g. ambient vs. heatwave years) and if that response differs between corals with contrasting bleaching phenotypes. Finally, the contribution of algal endosymbionts to acclimatization is being evaluated by exposing corals to a range of increasing temperatures in experimental mesocosms, potentially uncovering differences in the degree of acclimatization or sensitization for host vs. symbiont traits in corals with high fidelity (P. compressa) vs. cosmopolitan (M. capitata) symbiont associations. By understanding of the phenotypic diversity in thermal performance across biological scales, this research improves predictions of coral persistence in the face of the ongoing climate crisis.</span></p>
<p><span style="color:rgb(27, 27, 27); font-family:verdana; font-size:11.312px">This project is supported by the Biological Oceanography, Integrative Ecological Physiology, and Ocean Education Programs.</span></p>
<p><span style="color:rgb(27, 27, 27); font-family:verdana; font-size:11.312px">This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</span></p>
Coral acclimatization
largerWorkCitation
project
eng; USA
biota
oceans
2024-01-01
2024-08-11
Kaneohe Bay, Oahu, Hawaii
0
BCO-DMO catalogue of parameters from Physiological measurements from sea anemones exposed to normoxic or hypoxic conditions from lab experiments performed in Philadelphia, PA, USA in 2024
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/966314.rdf
Name: Sample_name
Units: unitless
Description: <p>Unique identifiers for experimental replicates</p>
http://lod.bco-dmo.org/id/dataset-parameter/966315.rdf
Name: Sample_ID
Units: unitless
Description: <p>Unique identifiers for experimental replicates</p>
http://lod.bco-dmo.org/id/dataset-parameter/966316.rdf
Name: Treatment
Units: unitless
Description: <p>Oxygen treatment to which adult animals were exposed (normoxia or hypoxia)</p>
http://lod.bco-dmo.org/id/dataset-parameter/966317.rdf
Name: Group
Units: unitless
Description: <p>Experimental group (biological replicates for each oxygen treatment)</p>
http://lod.bco-dmo.org/id/dataset-parameter/966318.rdf
Name: Sex
Units: unitless
Description: <p>Sex of animal(s) from which data arose</p>
http://lod.bco-dmo.org/id/dataset-parameter/966319.rdf
Name: Average_dry_weight
Units: grams
Description: <p>Dry weight expressed in grams</p>
http://lod.bco-dmo.org/id/dataset-parameter/966320.rdf
Name: Average_respiration
Units: umol O2 consumed per minute per g of dry weight
Description: <p>Aerobic respiration rate expressed in umol O2 consumed per minute per g of dry weight</p>
http://lod.bco-dmo.org/id/dataset-parameter/966321.rdf
Name: Average_eggs_dry_weight
Units: egg release per mg of adult animal dry weight
Description: <p>Female fecundity expressed as egg release per mg of adult animal dry weight</p>
http://lod.bco-dmo.org/id/dataset-parameter/966322.rdf
Name: Average_egg_volume
Units: um3
Description: <p>Egg volume expressed as um3</p>
http://lod.bco-dmo.org/id/dataset-parameter/966323.rdf
Name: Development_success_percent
Units: percent
Description: <p>Percent of larvae successfully developing past 3 days post-fertilization</p>
http://lod.bco-dmo.org/id/dataset-parameter/966324.rdf
Name: Average_relative_fitness
Units: unitless
Description: <p>Fitness relative to average of normoxia-exposed (i.e., control) individuals expressed in arbitrary units</p>
http://lod.bco-dmo.org/id/dataset-parameter/966325.rdf
Name: Average_sperm_M_dry_weight
Units: million (M) of sperm cells released per mg of adult animal dry weight
Description: <p>Male fecundity expressed as million (M) of sperm cells released per mg of adult animal dry weight</p>
http://lod.bco-dmo.org/id/dataset-parameter/966326.rdf
Name: Average_sperm_M_respiration
Units: nmol O2 consumed per minute per million (M) sperm cells
Description: <p>Aerboic respiration rate of sperm expressed as nmol O2 consumed per minute per million (M) sperm cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/966327.rdf
Name: Average_sperm_red_green_ratio
Units: unitless
Description: <p>Red to green fluorescence ratio of JC-1 dye used to determine sperm mitochondrial membrane potential</p>
http://lod.bco-dmo.org/id/dataset-parameter/966328.rdf
Name: Spawned_percent
Units: percent
Description: <p>Percent of animals that released gametes</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1369
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/af45566a-9a71-4eff-aad4-85b51185eea3/content
download
https://doi.org/10.26008/1912/bco-dmo.964167.1
download
onLine
dataset
<p><strong>Sea Anemone Collection and Culture</strong></p>
<p>Adult&nbsp;Nematostella vectensis&nbsp;sea anemones were collected from the JC NERR near Brigantine, New Jersey, USA in 2020.&nbsp;Spawning was induced approximately twice per month using a standard method for&nbsp;<em>Nematostella vectensis</em>&nbsp;(Hand and Uhlinger&nbsp;1992; Stefanik et&nbsp;al.&nbsp;2013).&nbsp;Larval anemones were retained in culture dishes and advanced to the adult stage over 2 years, then spawned in individual cups to identify female and male animals for use in the experiment.&nbsp;</p>
<p>After seven days of acclimation, adult anemones were distributed across 6 tubs (N = 3 tubs per DO treatment: normoxia/control and hypoxia) with 15 females and 14 males in each tub (N = 87 animals per treatment). Next, spawning was induced on day 7 of acclimation to clear anemones of developing gametes (Glass, Schmitt, et&nbsp;al.&nbsp;2023; Rivera et&nbsp;al.&nbsp;2021). Total water changes were then performed for each tub, and animals were placed back in ambient culture (i.e., normoxia) for the start of the experimental period (i.e., experimental day 0).&nbsp;</p>
<p><strong>Hypoxia Treatments</strong></p>
<p>Over nights 5, 6, and 7 of the experimental period, adult anemones were exposed to either normoxia (i.e., controls; DO = 7–10 mg L−1) or hypoxia (DO = 0.5–1.5 mg L−1) for ~12 h night−1. Nitrogen gas was used to deoxygenate the water in a glass jar filled nearly to the brim (500 mL; Ball Corporation, Westminster, CO, USA) to a value of ~1.5 mg L−1&nbsp;(salinity corrected) as determined by a handheld DO probe (YSI ProSolo 626650; accuracy = ±0.02 and precision = 0.01 mg L−1; YSI, Yellow Springs, OH, USA). The anemones from 1 culture tub (N = 29 animals) were transferred with minimal culture water (containing no&nbsp;Artemia) into the jar, which was then topped off with anoxic 12 ppt ASW, capped, and covered with plastic wrap secured by a rubber band. This process was repeated for the 5 other jars, skipping the seawater deoxygenation step for the 3 normoxia jars, and all 6 jars were placed in a dark incubator at 18°C. While anemones were in the treatment jars, the (normoxic) seawater from the culture tubs was pooled and filtered (mesh size 100 μm) before being redistributed across the tubs to minimise block effects by tub and maintain water cleanliness by removing particulate waste, though this may have introduced a confounding factor and should be considered when interpreting the data.</p>
<p>After ~12 h, the jars were opened and a DO reading was quickly recorded. Anemones were then transferred back into their respective tubs with minimal water carried over from the treatment jars. Both animals exposed to hypoxia and controls were handled in the same manner.</p>
<p><strong>Measurements</strong></p>
<p>Detailed methods can be found in the associated publication (Glass et al. 2025).</p>
<ul>
<li>Dry weight of adult sea anemones was determined using a drying oven and lab balance.</li>
<li>Respiration rates of adult and larval sea anemones were determined via respirometry using a PreSens sensor dish reader system.</li>
<li>Female fecundity was determined by counting eggs imaged via brightifled microscopy.</li>
<li>Male fecundity was determined by counting sperm via flow cytometry.</li>
<li>Egg size was determined by measuring eggs in images collected via brightfield microscopy.</li>
<li>Sperm mitochondrial membrane potential was determined using the fluorescent dye JC-1 analyzed via flow cytometry.</li>
<li>Larval developmental success and settlement rates were determined via visual inspection under brightfield microscopy.</li>
</ul>
Specified by the Principal Investigator(s)
<p>Full details regarding data and statistical analyses are present in Glass et al.&nbsp;(2025), Supporting Information.&nbsp;RStudio with R version 4.2.1 was used for all analyses (RStudio Team&nbsp;2020).&nbsp;All values are expressed as averages rounded to appropriate significant figures ± standard error of the mean (SEM), and all original data is attached as supplemental files and published (Glass, B., &amp; Barott, K. 2025), as referenced in the related datasets section. The code used (Glass, B., &amp; Barott, K. 2025) is listed in the related publication section.&nbsp;</p>
Specified by the Principal Investigator(s)
- Imported "Combined_physiology_data.csv" into the BCO-DMO system
- Rename parameter names to remove units, in keeping with BCO-DMO style
- Exported file as "964167_v1_sea_anemones_measurements"
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
drying oven
drying oven
PI Supplied Instrument Name: drying oven PI Supplied Instrument Description:Dry weight of adult sea anemones was determined using a drying oven and lab balance. Instrument Name: Drying Oven Instrument Short Name: Instrument Description: a heated chamber for drying
Cytek guava flow cytometer
Cytek guava flow cytometer
PI Supplied Instrument Name: Cytek guava flow cytometer PI Supplied Instrument Description:Male fecundity was determined by counting sperm via flow cytometry. Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
Microscope
Microscope
PI Supplied Instrument Name: Microscope PI Supplied Instrument Description:Female fecundity was determined by counting eggs imaged via brightifled microscopy. Egg size was determined by measuring eggs in images collected via brightfield microscopy. Larval developmental success and settlement rates were determined via visual inspection under brightfield microscopy. Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
SensorDish Reader from Precision Sensing
SensorDish Reader from Precision Sensing
PI Supplied Instrument Name: SensorDish Reader from Precision Sensing PI Supplied Instrument Description:Respiration rates of adult and larval sea anemones were determined via respirometry using a PreSens sensor dish reader system. Instrument Name: Respirometer Instrument Short Name: Instrument Description: A device that measures the rate of respiration by a living organism or organic system by measuring its rate of exchange of oxygen and/or carbon dioxide.
lab balance
lab balance
PI Supplied Instrument Name: lab balance PI Supplied Instrument Description:Dry weight of adult sea anemones was determined using a drying oven and lab balance. Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/