http://lod.bco-dmo.org/id/dataset/964174
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-06-09
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Targeted metabolomics data from coral and sea anemone larvae exposed to normoxic or hypoxic conditions in lab and field experiments performed in USA and Bermuda during 2023-2024
2025-06-10
publication
2025-06-10
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-07-08
publication
https://doi.org/10.26008/1912/bco-dmo.964174.1
Katie Barott
University of Pennsylvania
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Glass, B., Barott, K. (2025) Targeted metabolomics data from coral and sea anemone larvae exposed to normoxic or hypoxic conditions in lab and field experiments performed in USA and Bermuda during 2023-2024. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-06-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.964174.1 [access date]
Targeted metabolomics data from coral and sea anemone larvae exposed to normoxic or hypoxic conditions Dataset Description: Methods and Sampling: <p><strong>Adult culture and spawning</strong></p>
<p>Adult&nbsp;<em>Nematostella vectensis</em>&nbsp;sea anemones were collected from a salt marsh in Brigantine, New Jersey in the fall of 2020, and spawning was induced. Larvae (n = 1 cohort with mixed parentage) were then cultured to the planula stage (3 d post-fertilization) for experimentation. An aquarium population of adult&nbsp;<em>Galaxea fascicularis</em>&nbsp;colonies (n = 9 females, 10 males) spawned during August 2023 at Carnegie Science (Baltimore, MD, USA), yielding a cohort of planulae (n = 1 cohort with mixed parentage) that were used in experiments within 48 h. Adult&nbsp;P<em>orites astreoides</em>&nbsp;colones (n = 20) were collected in Bermuda (32°22′13″N, 64°44′27″W) during July 2023. Brooded planulae (n = 4 cohorts) were collected&nbsp;and maintained in culture for ~ 48 h.</p>
<p>At the time of use in experimentation, larvae from all 3 species were in the planula stage and would become competent to settle within 72–96 h (Hand and Uhlinger&nbsp;1992; Goodbody-Gringley et al.&nbsp;2018; Wei et al.&nbsp;2023). Artificial seawater was used for culturing and experimentation for&nbsp;<em>N. vectensis</em>&nbsp;and&nbsp;<em>G. fascicularis</em>, while flow-through, natural seawater facilities at the Bermuda Institute for Ocean Science were used for&nbsp;<em>P. astreoides</em>.</p>
<p><strong>Dissolved oxygen treatments and larval sampling</strong></p>
<p>Stage-matched, swimming planulae of <em>Nematostella vectensis</em>, <em>Galaxea fascicularis</em>, and <em>Porites astreoides</em> (N = 1,200–2,400 larvae species<sup>-1</sup>) were divided into six replicate groups (three normoxia/control and three hypoxia) and exposed to 6 h of normoxia (dissolved oxygen (DO) = 6.8–8.69 mg L<sup>-1</sup>) or severe hypoxia (DO = 1.58–1.8 mg L<sup>-1</sup>; seawater deoxygenated using N<sub>2</sub> gas) inside sealed glass jars (500 mL) overnight from 21:00 h to 03:00 h the following day. The jars were placed at the ambient culture temperature for each species (<em>N. vectensis</em>: 18°C; <em>G. fascicularis</em>: 27°C; <em>P. astreoides</em>: 28°C). At the end of the treatment period, the jars were uncapped and groups of 20–30 larvae (N = 60–90 larvae treatment<sup>-1</sup> species<sup>-1</sup>) were transferred to 1.5 mL tubes without seawater for storage at -80°C until processing for targeted metabolomics as described below.</p>
<p><strong>Targeted metabolics via liquid chromatography-mass spectrometry</strong></p>
<p>Groups of frozen larvae were thawed on ice and homogenized in 160 µL of 50:50 0.3% formic acid/acetonitrile in tough microorganism tubes (Revvity, Waltham, MA, USA) at 4°C in a Precellys homogenizer (Bertin Technologies, France). Aliquots of homogenates (20 µL) were extracted with organic solvents for individual targeted liquid chromatography-mass spectrometry (LC-MS) metabolomics assays (acylcarnitines, amino acids, organic acids, and nucleotides). A 10 µL aliquot of each homogenate was also used for protein concentration determination (to normalize metabolite concentrations) via the Bradford method using a bovine albumin serum standard curve. Quantitation of metabolites was achieved using multiple reaction monitoring of calibration solutions and study samples on an Agilent 1290 Infinity UHPLC/6495 triple quadrupole mass spectrometer at the University of Pennsylvania Metabolomics Core (RRID:SCR_022381). Raw data were processed using Mass Hunter quantitative analysis software. Calibration curves (R<sup>2</sup> = 0.99 or greater) were fitted with a linear or a quadratic curve with a 1/X or 1/X<sup>2</sup> weighting.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2237658 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2237658
completed
Katie Barott
University of Pennsylvania
215-746-6911
433 South University Avenue Leidy Laboratories 326
Philadelphia
PA
19104
USA
kbarott@sas.upenn.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Species
Treatment
Cohort
Group
Life_stage
Hours_post_treatment
ADP
AMP
ATP
cAMP
CMP
CTP
GDP
GMP
GTP
ITP
NAD
NADP
NAM
NMN
TTP
UMP
Methylhistidine_1
Methylhistidine_3
Alanine
Arginine
Asparagine
Aspartate
Citrulline
Dimethylarginine_ADMA
Dimethylarginine_SDMA
Glutamate
Glutamine
Glycine
Histidine
Homocitrulline
Isoleucine
Leucine
Lysine
Methionine
Ornithine
Phenylalanine
Proline
Serine
Threonine
Tryptophan
Tyrosine
Valine
C02
C03
C03_DC
C04_Butyryl
C04_Isobutyryl
C04_1
C04_DC_MeMal
C04_DC_Succinyl
C04_OH_Butyryl
C04_OH_Isobutyryl
C05_2_Methylbutyryl
C05_Isovaleryl
C05_1
C05_DC
C05_OH
C06
C06_OH
C08
C08_1_OH
C08_OH
C10
C10_OH
C12
C14
C14_1
C14_OH
C16
C16_1
C16_OH
C18
C18_1
HBA_3
alpha_Ketoglutaric_Acid
Citrate
Fumarate
Lactate
Malate
Pyruvate
Succinate
AphiaID
LSID
homogenized
6495 triple quadrupole mass spectrometer
Agilent 1290 Infinity UHPLC
theme
None, User defined
species scientific name (binomial)
treatment
sample description
replicate
stage
time_point
abundance
taxon_code
Life Science Identifier (LSID)
featureType
BCO-DMO Standard Parameters
Homogenizer
Mass Spectrometer
Ultra-high-performance liquid chromatography
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
CAREER: Helping or hindering? Determining the influence of repetitive marine heatwaves on acclimatization of reef-building corals across biological scales
https://osprey.bco-dmo.org/project/925169
CAREER: Helping or hindering? Determining the influence of repetitive marine heatwaves on acclimatization of reef-building corals across biological scales
<p><em>NSF Award Abstract:</em></p>
<p><span style="color:rgb(27, 27, 27); font-family:verdana; font-size:11.312px">Ocean warming driven by climate change has led to staggering losses of coral on reefs worldwide and is now among the most pressing of stressors threatening the survival of coral reef ecosystems today. As marine heatwaves associated with ocean warming become increasingly frequent, it is urgent to understand if and how reef-building corals will be able to respond to these repeat stress events and thus survive in a rapidly warming ocean. To address this problem, this project is investigating how corals on the reef respond to recurring marine heatwaves in order to identify if repeat exposure to heat stress promotes coral tolerance of higher temperatures via acclimatization or instead leads to the accumulation of stress and thus reduced performance and survival following future stress. The results of this study are critical for understanding how the current generation of corals will respond to increasingly warmer oceans, and whether acclimatization will buy enough individuals sufficient time for adaptation to occur and promote coral persistence into the future. In addition, this project is training students from secondary schools through advanced postdoctoral researchers in global change biology and ecology. Specifically, the investigators are increasing access to research opportunities for undergraduate students by developing a new hands-on course-based undergraduate research experience (CURE) in Global Ocean Change Biology that will reach hundreds of students per year. Outreach efforts include creation of hands-on coral reefs and climate change activities for incoming first-generation, low-income undergraduate students and a professional development program to train middle and high-school teachers to deploy these climate change activities in their classrooms in the Philadelphia Public School District.</span></p>
<p><span style="color:rgb(27, 27, 27); font-family:verdana; font-size:11.312px">Acclimatization following exposure to sub-lethal heat stress may be an important protective mechanism for corals to survive a changing climate. However, the role of environmental memory of marine heatwaves in driving acclimatization or, conversely, stress accumulation and sensitization of reef-building corals is not well understood. This study is addressing this question using a combination of in situ and mesocosm experiments to assess the cellular, organismal, and ecological consequences of repeat heatwaves on corals with contrasting bleaching histories. Specifically, the researchers are monitoring adjacent conspecific pairs of bleaching-susceptible and bleaching-resistant individuals of two reef-building coral species in Hawaii, Montipora capitata and Porites compressa. These corals have been monitored for over 7 years through multiple bleaching events and are being used to test the hypothesis that environmental memory of marine heatwaves differentially alters coral thermal performance due to phenotypic variation in acclimatization ability within and between species. This work is identifying whether the bleaching thresholds of corals with different bleaching histories varies through time, and the consequences of these phenotypes on coral calcification, survival, and population size structure are being assessed using a combination of benthic surveys, photogrammetry, and in situ growth measurements. The influence of environmental memory of heatwaves on coral physiology is being assessed using thermal performance curves to determine how the thermal optima of respiration, photosynthesis, calcification, and host intracellular pH change (or not) over time (e.g. ambient vs. heatwave years) and if that response differs between corals with contrasting bleaching phenotypes. Finally, the contribution of algal endosymbionts to acclimatization is being evaluated by exposing corals to a range of increasing temperatures in experimental mesocosms, potentially uncovering differences in the degree of acclimatization or sensitization for host vs. symbiont traits in corals with high fidelity (P. compressa) vs. cosmopolitan (M. capitata) symbiont associations. By understanding of the phenotypic diversity in thermal performance across biological scales, this research improves predictions of coral persistence in the face of the ongoing climate crisis.</span></p>
<p><span style="color:rgb(27, 27, 27); font-family:verdana; font-size:11.312px">This project is supported by the Biological Oceanography, Integrative Ecological Physiology, and Ocean Education Programs.</span></p>
<p><span style="color:rgb(27, 27, 27); font-family:verdana; font-size:11.312px">This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</span></p>
Coral acclimatization
largerWorkCitation
project
eng; USA
biota
oceans
2023-06-01
2024-11-01
Kaneohe Bay, Oahu, Hawaii
0
BCO-DMO catalogue of parameters from Targeted metabolomics data from coral and sea anemone larvae exposed to normoxic or hypoxic conditions in lab and field experiments performed in USA and Bermuda during 2023-2024
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/964346.rdf
Name: Species
Units: units
Description: <p>Species from which metabolomics data originate</p>
http://lod.bco-dmo.org/id/dataset-parameter/964347.rdf
Name: Treatment
Units: units
Description: <p>Experimental treatment (normoxia or hypoxia)</p>
http://lod.bco-dmo.org/id/dataset-parameter/964348.rdf
Name: Cohort
Units: units
Description: <p>Spawning cohort from which larvae originate</p>
http://lod.bco-dmo.org/id/dataset-parameter/964349.rdf
Name: Group
Units: units
Description: <p>Replicate group within the experimental treatment (1-3)</p>
http://lod.bco-dmo.org/id/dataset-parameter/964350.rdf
Name: Life_stage
Units: units
Description: <p>Life stage; always "larva"</p>
http://lod.bco-dmo.org/id/dataset-parameter/964351.rdf
Name: Hours_post_treatment
Units: hours
Description: <p>Time point at which larvae were collected for metabolomics; here, always = 0</p>
http://lod.bco-dmo.org/id/dataset-parameter/964352.rdf
Name: ADP
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964353.rdf
Name: AMP
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964354.rdf
Name: ATP
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964355.rdf
Name: cAMP
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964356.rdf
Name: CMP
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964357.rdf
Name: CTP
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964358.rdf
Name: GDP
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964359.rdf
Name: GMP
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964360.rdf
Name: GTP
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964361.rdf
Name: ITP
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964362.rdf
Name: NAD
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964363.rdf
Name: NADP
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964364.rdf
Name: NAM
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964365.rdf
Name: NMN
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964366.rdf
Name: TTP
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964367.rdf
Name: UMP
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964368.rdf
Name: Methylhistidine_1
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964369.rdf
Name: Methylhistidine_3
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964370.rdf
Name: Alanine
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964371.rdf
Name: Arginine
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964372.rdf
Name: Asparagine
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964373.rdf
Name: Aspartate
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964374.rdf
Name: Citrulline
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964375.rdf
Name: Dimethylarginine_ADMA
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964376.rdf
Name: Dimethylarginine_SDMA
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964377.rdf
Name: Glutamate
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964378.rdf
Name: Glutamine
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964379.rdf
Name: Glycine
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964380.rdf
Name: Histidine
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964381.rdf
Name: Homocitrulline
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964382.rdf
Name: Isoleucine
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964383.rdf
Name: Leucine
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964384.rdf
Name: Lysine
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964385.rdf
Name: Methionine
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964386.rdf
Name: Ornithine
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964387.rdf
Name: Phenylalanine
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964388.rdf
Name: Proline
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964389.rdf
Name: Serine
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964390.rdf
Name: Threonine
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964391.rdf
Name: Tryptophan
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964392.rdf
Name: Tyrosine
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964393.rdf
Name: Valine
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964394.rdf
Name: C02
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964395.rdf
Name: C03
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964396.rdf
Name: C03_DC
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964397.rdf
Name: C04_Butyryl
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964398.rdf
Name: C04_Isobutyryl
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964399.rdf
Name: C04_1
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964400.rdf
Name: C04_DC_MeMal
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964401.rdf
Name: C04_DC_Succinyl
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964402.rdf
Name: C04_OH_Butyryl
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964403.rdf
Name: C04_OH_Isobutyryl
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964404.rdf
Name: C05_2_Methylbutyryl
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964405.rdf
Name: C05_Isovaleryl
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964406.rdf
Name: C05_1
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964407.rdf
Name: C05_DC
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964408.rdf
Name: C05_OH
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964409.rdf
Name: C06
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964410.rdf
Name: C06_OH
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964411.rdf
Name: C08
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964412.rdf
Name: C08_1_OH
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964413.rdf
Name: C08_OH
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964414.rdf
Name: C10
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964415.rdf
Name: C10_OH
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964416.rdf
Name: C12
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964417.rdf
Name: C14
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964418.rdf
Name: C14_1
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964419.rdf
Name: C14_OH
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964420.rdf
Name: C16
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964421.rdf
Name: C16_1
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964422.rdf
Name: C16_OH
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964423.rdf
Name: C18
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964424.rdf
Name: C18_1
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964425.rdf
Name: HBA_3
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964426.rdf
Name: alpha_Ketoglutaric_Acid
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964427.rdf
Name: Citrate
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964428.rdf
Name: Fumarate
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964429.rdf
Name: Lactate
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964430.rdf
Name: Malate
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964431.rdf
Name: Pyruvate
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964432.rdf
Name: Succinate
Units: nmol mg protein-1
Description: <p>Abundance of each metabolite in each sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/964484.rdf
Name: AphiaID
Units: unitless
Description: <p>AphiaID of species from which metabolomics data originate</p>
http://lod.bco-dmo.org/id/dataset-parameter/964485.rdf
Name: LSID
Units: unitless
Description: <p>LSID of species from which metabolomics data originate</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
27241
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/29cb57ba-eea0-4a9f-ae48-95356f25ceff/content
download
https://doi.org/10.26008/1912/bco-dmo.964174.1
download
onLine
dataset
<p><strong>Adult culture and spawning</strong></p>
<p>Adult&nbsp;<em>Nematostella vectensis</em>&nbsp;sea anemones were collected from a salt marsh in Brigantine, New Jersey in the fall of 2020, and spawning was induced. Larvae (n = 1 cohort with mixed parentage) were then cultured to the planula stage (3 d post-fertilization) for experimentation. An aquarium population of adult&nbsp;<em>Galaxea fascicularis</em>&nbsp;colonies (n = 9 females, 10 males) spawned during August 2023 at Carnegie Science (Baltimore, MD, USA), yielding a cohort of planulae (n = 1 cohort with mixed parentage) that were used in experiments within 48 h. Adult&nbsp;P<em>orites astreoides</em>&nbsp;colones (n = 20) were collected in Bermuda (32°22′13″N, 64°44′27″W) during July 2023. Brooded planulae (n = 4 cohorts) were collected&nbsp;and maintained in culture for ~ 48 h.</p>
<p>At the time of use in experimentation, larvae from all 3 species were in the planula stage and would become competent to settle within 72–96 h (Hand and Uhlinger&nbsp;1992; Goodbody-Gringley et al.&nbsp;2018; Wei et al.&nbsp;2023). Artificial seawater was used for culturing and experimentation for&nbsp;<em>N. vectensis</em>&nbsp;and&nbsp;<em>G. fascicularis</em>, while flow-through, natural seawater facilities at the Bermuda Institute for Ocean Science were used for&nbsp;<em>P. astreoides</em>.</p>
<p><strong>Dissolved oxygen treatments and larval sampling</strong></p>
<p>Stage-matched, swimming planulae of <em>Nematostella vectensis</em>, <em>Galaxea fascicularis</em>, and <em>Porites astreoides</em> (N = 1,200–2,400 larvae species<sup>-1</sup>) were divided into six replicate groups (three normoxia/control and three hypoxia) and exposed to 6 h of normoxia (dissolved oxygen (DO) = 6.8–8.69 mg L<sup>-1</sup>) or severe hypoxia (DO = 1.58–1.8 mg L<sup>-1</sup>; seawater deoxygenated using N<sub>2</sub> gas) inside sealed glass jars (500 mL) overnight from 21:00 h to 03:00 h the following day. The jars were placed at the ambient culture temperature for each species (<em>N. vectensis</em>: 18°C; <em>G. fascicularis</em>: 27°C; <em>P. astreoides</em>: 28°C). At the end of the treatment period, the jars were uncapped and groups of 20–30 larvae (N = 60–90 larvae treatment<sup>-1</sup> species<sup>-1</sup>) were transferred to 1.5 mL tubes without seawater for storage at -80°C until processing for targeted metabolomics as described below.</p>
<p><strong>Targeted metabolics via liquid chromatography-mass spectrometry</strong></p>
<p>Groups of frozen larvae were thawed on ice and homogenized in 160 µL of 50:50 0.3% formic acid/acetonitrile in tough microorganism tubes (Revvity, Waltham, MA, USA) at 4°C in a Precellys homogenizer (Bertin Technologies, France). Aliquots of homogenates (20 µL) were extracted with organic solvents for individual targeted liquid chromatography-mass spectrometry (LC-MS) metabolomics assays (acylcarnitines, amino acids, organic acids, and nucleotides). A 10 µL aliquot of each homogenate was also used for protein concentration determination (to normalize metabolite concentrations) via the Bradford method using a bovine albumin serum standard curve. Quantitation of metabolites was achieved using multiple reaction monitoring of calibration solutions and study samples on an Agilent 1290 Infinity UHPLC/6495 triple quadrupole mass spectrometer at the University of Pennsylvania Metabolomics Core (RRID:SCR_022381). Raw data were processed using Mass Hunter quantitative analysis software. Calibration curves (R<sup>2</sup> = 0.99 or greater) were fitted with a linear or a quadratic curve with a 1/X or 1/X<sup>2</sup> weighting.</p>
Specified by the Principal Investigator(s)
<p>Raw data were processed using Mass Hunter quantitative analysis software. Calibration curves (R<sup>2</sup> = 0.99 or greater) were fitted with a linear or a quadratic curve with a 1/X or 1/X<sup>2</sup> weighting.</p>
Specified by the Principal Investigator(s)
- Imported "Metabolomics_data.csv" into the BCO-DMO system
- Renamed fields to comply with system and style requirements (moving number from first character)
- Exported file as "964174_v1_targeted_metabolomics"
Scientific names in the data were checked using World Register of Marine Species (WoRMS) Taxon Match. All scientific names in the data are valid and accepted names as of 2025-06-10.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
homogenized
homogenized
PI Supplied Instrument Name: homogenized PI Supplied Instrument Description:Groups of frozen larvae were thawed on ice and homogenized in 160 µL of 50:50 0.3% formic acid/acetonitrile in tough microorganism tubes (Revvity, Waltham, MA, USA) at 4°C in a Precellys homogenizer (Bertin Technologies, France). Instrument Name: Homogenizer Instrument Short Name:Homogenizer Instrument Description: A homogenizer is a piece of laboratory equipment used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others.
6495 triple quadrupole mass spectrometer
6495 triple quadrupole mass spectrometer
PI Supplied Instrument Name: 6495 triple quadrupole mass spectrometer PI Supplied Instrument Description: Quantitation of metabolites was achieved using multiple reaction monitoring of calibration solutions and study samples on an Agilent 1290 Infinity UHPLC/6495 triple quadrupole mass spectrometer at the University of Pennsylvania Metabolomics Core (RRID:SCR_022381). Raw data were processed using Mass Hunter quantitative analysis software. Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Agilent 1290 Infinity UHPLC
Agilent 1290 Infinity UHPLC
PI Supplied Instrument Name: Agilent 1290 Infinity UHPLC PI Supplied Instrument Description:Quantitation of metabolites was achieved using multiple reaction monitoring of calibration solutions and study samples on an Agilent 1290 Infinity UHPLC/6495 triple quadrupole mass spectrometer at the University of Pennsylvania Metabolomics Core (RRID:SCR_022381). Raw data were processed using Mass Hunter quantitative analysis software. Instrument Name: Ultra-high-performance liquid chromatography Instrument Short Name:UHPLC Instrument Description: Ultra high-performance liquid chromatography: Column chromatography where the mobile phase is a liquid, the stationary phase consists of very small (< 2 microm) particles and the inlet pressure is relatively high.