http://lod.bco-dmo.org/id/dataset/965557 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2025-06-18 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Results of incurrent/excurrent water samples taken from three sponge species from the Florida Keys (USA) over two or three days using a modified version of the vacuSIP in January 2022 2025-08-26 publication 2025-08-26 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2025-11-13 publication https://doi.org/10.26008/1912/bco-dmo.965557.1 Amy Apprill Woods Hole Oceanographic Institution principalInvestigator Cole G. Easson Middle Tennessee State University principalInvestigator Cara L. Fiore Appalachian State University principalInvestigator Alicia M. Reigel Appalachian State University principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Fiore, C. L., Easson, C. G., Michaels, E. C., Reigel, A. M., Apprill, A. (2025) Results of incurrent/excurrent water samples taken from three sponge species from the Florida Keys (USA) over two or three days using a modified version of the vacuSIP in January 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-08-26 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.965557.1 [access date] Sponge 3day VacuSIP Jan 2022 Dataset Description: Methods and Sampling: &lt;p&gt;Incurrent/excurrent water samples were taken from three sponge species from the Florida Keys (USA) over two or three days using a modified version of the vacuSIP (Morganti et al. 2016). Briefly, acid-cleaned and combusted bottles were negatively pressurized and sealed. PEEK tubing lines were connected to the pressurized bottles via a needle, and this pulled seawater from the opening of the sponge (&quot;excurrent&quot;) or nearby seawater (&quot;incurrent&quot;) at a slow and steady rate to fill the bottles. Nutrient data from the seawater samples were compiled and organized by sponge species and water type (incurrent or excurrent) in an Excel file. Seawater samples from two sponge species (&lt;em&gt;Niphates digitalis&lt;/em&gt; and &lt;em&gt;Verongula rigida&lt;/em&gt;) were filtered through 0.2-micrometer (µm) polyethersulfone filters, while seawater from the sponge &lt;em&gt;Xestspongia muta&lt;/em&gt; was filtered through 0.2 µm Teflon Omnipore filters. Filtrate was then saved for inorganic nutrients and those from &lt;em&gt;X. muta&lt;/em&gt; were processed for dissolved organic matter composition using targeted metabolomics analysis. Flow cytometry of phytoplankton and bacteria data was collected from pre-filtered seawater and preserved in 0.5% paraformaldehyde final concentration. Seawater for total organic carbon (TOC) and total nitrogen (TN) was also collected prior to filtration and was acidified to ~pH 2 using concentrated HCl. Metabolomics and TOC analysis were performed at Woods Hole Oceanographic Institution at the Mass Spectrometry Facility.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Dissolved Organic Matter Extraction:&lt;/strong&gt;&lt;br /&gt; Filtered seawater was processed using PPL solid phase extraction following the protocol by Dittmar et al. (2008). Extracts were dried to nearly completeness, leaving a small viscous drop in the vial. These extracts were then shipped to WHOI for metabolomics analysis.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Targeted Metabolite Analysis by UPLC-MS:&lt;/strong&gt;&lt;br /&gt; DOM extracts were reconstituted in 200 microliters (μl) MilliQ water with 50 nanograms per milliliter (ng/ml) isotopically-labeled injection standards d2 biotin, d6 succinic acid, d4 cholic acid, and d7 indole 3 acetic acid. We used ultra-performance liquid chromatography (Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific) coupled to a heated electrospray ionization source (H-ESI) and a triple quadrupole mass spectrometer (TSQ Vantage, Thermo Scientific) operated under selected reaction monitoring (SRM) mode. We performed chromatographic separation with a Waters Acquity HSS T3 column (2.1 × 100 millimeters (mm), 1.8 μm) equipped with a Vanguard pre-column and maintained at 40 degrees Celsius (°C). We eluted the metabolites from the column with (A) 0.1% formic acid in water and (B) 0.1% formic acid in acetonitrile at a flow rate of 0.5 milliliters per minute (mL min-1), according to the gradient: 0 min, 1% B; 1 min, 1%B; 3 min, 15%B; 6 min, 50%B; 9 min, 95%B; 10 min, 95%B; 10.2 min, 1%B; 12 min, 1%B (total run time = 12 min). Settings for source gases were 55 (sheath), 20 (auxiliary) and 0 (sweep), and these settings are presented in arbitrary units. The heated capillary temperature was 375 °C and the vaporizer temperature was 400 °C. For positive and negative modes, we performed separate autosampler injections of 5 μL each.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Flow Cytometry:&lt;/strong&gt;&lt;br /&gt; Samples were preserved and stored at -80°C until later batch analysis at the University of Hawaii SOEST Flow Cytometry Facility (&lt;a href=&quot;http://www.soest.hawaii.edu/sfcf&quot; target=&quot;_blank&quot;&gt;www.soest.hawaii.edu/sfcf&lt;/a&gt;). Microbial cells were enumerated using flow cytometry (Selph, 2021). In brief, samples (0.1 mL) were thawed in batches, stained with the DNA dye Hoechst 34580 (1 microgram per milliliter (µg/mL) final), then run at 30 microliters per minute (µL min-1) on a Beckman-Coulter CytoFlex S flow cytometer, using lasers emitting at 375 nanometers (nm) (to detect Hoechst), 488 nm (for scatter and chlorophyll parameters), and 561 nm (for phycoerythrin). Resulting listmode files (FCS 3.0) were analyzed using FlowJo software (Becton Dickinson, v. 10.8.2) to distinguish microbial populations based on their fluorescence signals (chlorophyll, phycoerythrin, DNA), as well as forward and right-angle light scatter. Heterotrophic bacteria were distinguished from phytoplankton by their DNA signature and absence of pigment. &lt;em&gt;Prochlorococcus&lt;/em&gt; and &lt;em&gt;Synechococcus&lt;/em&gt; were separated from larger eukaryotic phytoplankton by their light scatter signatures, as well as their characteristic pigment and DNA signatures. Other phytoplankton (eukaryotes, mostly 2-20 µm pico- and nano-sized cells given the small volume analyzed) had higher light scatter and more chlorophyll fluorescence per cell.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Inorganic nutrients:&lt;/strong&gt;&lt;br /&gt; Inorganic nutrients included phosphate, nitrate+nitrite, nitrite, ammonia, and silicic acid. The phosphate method is a modification of the molybdenum blue procedure of Bernhardt and Wilhelms (1967), in which phosphate is determined as reduced phosphomolybdic acid employing hydrazine as the reductant. The nitrate + nitrite analysis uses the basic method of Armstrong et al. (1967), with modifications to improve the precision and ease of operation. Sulfanilamide and N-(1-Napthyl)ethylenediamine dihydrochloride react with nitrite to form a colored diazo compound. For the nitrate + nitrite analysis, nitrate is first reduced to nitrite using an OTCR and imidazole buffer as described by Patton (1983). Nitrite analysis is performed on a separate channel, omitting the cadmium reductor and the buffer. The method is based on that of Armstrong et al. (1967) as adapted by Atlas et al. (1971). The addition of an acidic molybdate reagent forms silicomolybdic acid, which is then reduced by stannous chloride. This indophenol blue method is modified from ALPKEM RFA methodology which references Methods for Chemical Analysis of Water and Wastes, March 1984, EPA-600/4-79-020, &quot;Nitrogen Ammonia&quot;, Method 350.1 (Colorimetric, Automated Phenate) A detailed description of the continuous segmented flow procedures used can be found in Gordon et. al. (1994).&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1924540 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1924540 completed Amy Apprill Woods Hole Oceanographic Institution 508-289-2649 Marine Chemistry and Geochemistry Department 266 Woods Hole Road, MS# 4 Woods Hole MA 02543 USA aapprill@whoi.edu pointOfContact Cole G. Easson Middle Tennessee State University 615-898-5822 1672 Greenland Drive SCI 2044 Murfreesboro TN 37132 USA Cole.Easson@mtsu.edu pointOfContact Cara L. Fiore Appalachian State University 828-262-7548 572 Rivers St Boone NC 28607 USA fiorec@appstate.edu pointOfContact Alicia M. Reigel Appalachian State University Lexington VA USA areigel@wlu.edu pointOfContact asNeeded Dataset Version: 1 Unknown Sponge_species Replicate_number Water_type Day Site Depth Volume Date Latitude Longitude Total_sample_RU_after_blank_subtraction M_C_ratio BIX HIX FI Ultra_Violet_Humic_like Marine_Humic_like Visible_Humic_like Tryptophan_like Tyrosine_like Phenylalanine_like Lignin_like TOC TN Phosphate Nitrate_plus_Nitrite Silicate Nitrite Ammonium Prochlorococcus Synechococcus LowPE_Synechococcus Picoeukaryotes Heterotrophic_bacteria _2_3_dihydroxypropane_1_sulfonate _2_3_dihydroxybenzoic_acid _2prime_deoxycytidine _2_methyl_4_oxopentanoic _2_Keto_3_deoxy_6_phosphogluconate _3_methyl_2_oxobutanoic_acid _4_aminobenzoic_acid _4_methyl_2_oxopentanoic_acid _6_hydroxymelatonin adenine adenosine_5prime_monophosphate adenosine alanine_isom_sarcosine _4_amino_5_aminomethyl_2_methylpyrimidine anthranilate arginine argininosuccinic_acid caffeine carnitine choline ciliatine citric_acid citrulline cyanocobalamin cysteine cytidine cytosine desthiobiotin chitobiose dimethylsulfoniopropionate D_fructose_1_6_bisphosphate fructose_6_phosphate fumaric_acid gamma_aminobutyric_acid D_glucosamine glucose_6_phosphate glutamic_acid glutamine glutathione sn_glycerol_3_phosphate glycine glycine_betaine guanine guanosine _4_methyl_5_thiazoleethanol histadine histidinol homocysteine hydroxocobalamin hydroxyproline hypoxanthine inosine inosine_5prime_monophosphate isoleucine kynurenine leucine lumichrome malic_acid methionine methylmalonic_acid _5prime_methylthioadenosine n_acetyl_glucosamine N_acetyl_D_glucosamine_6_phosphate n_acetyl_glutamic_acid n_acetyl_muramic_acid phosphoserine ornithine pantothenic_acid phenylalanine proline riboflavin D_Ribose_5_phosphate serine taurocholic_acid spermidine succinic_acid sucrose syringic_acid thiamine threonine_homoserine thymidine chitotriose tryptophan tyrosine uric_acid uridine uridine_5prime_monophosphate valine xanthine xanthosine Alpkem RFA 300 Beckman-Coulter CytoFLEX S flow cytometer Technicon AutoAnalyzer II ultra-performance liquid chromatography (Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific) theme None, User defined species scientific name (binomial) replicate sample description time_point site depth volume date latitude longitude ratio No BCO-DMO term total organic Carbon Nitrogen phosphate nitrate plus nitrite Silicate, Si(OH)4, silicic acid Nitrite Ammonium abundance metabolite featureType BCO-DMO Standard Parameters Alpkem RFA300 Flow Cytometer Technicon AutoAnalyzer II Ultra-high-performance liquid chromatography instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: The Influence of Sponge Holobiont Metabolism on Coral Reef Dissolved Organic Matter and Reef Microorganisms https://osprey.bco-dmo.org/project/822468 Collaborative Research: The Influence of Sponge Holobiont Metabolism on Coral Reef Dissolved Organic Matter and Reef Microorganisms <p><em>NSF Award Abstract:</em><br /> The seawater around coral reefs is typically low in nutrients, yet coral reefs are teeming with life and are often compared to oases in a desert. Life exists in these 'marine deserts' in large part, due to symbiotic associations between single-celled microbes and invertebrates such as corals and sponges. The concentration and type of dissolved organic matter (DOM), a complex pool of organic nutrients such as amino acids, vitamins, and other diverse compounds, also affects the health of coral reefs. The composition of DOM on coral reefs is linked to both the composition of free-living microbes in the seawater and to the nutrition of filter-feeding organisms, such as corals and sponges. However, the factors that influence the composition of DOM on coral reefs and the consequences of how it changes are not well understood. Recent work suggests that sponges could have a significant impact on the composition of reef dissolved organic nutrients, depending on sponge species due to differences in filtration capacity and in their symbiotic microbial communities. This project characterizes how diverse sponge species process DOM on coral reefs and determines the impacts of this processing on the free-living microbial community. Seawater is collected from sponges (pre- and post- sponge filtration) on coral reefs in the relatively pristine region of Curacao, and incubation experiments measure the impact of sponge filtration on the growth of the free-living microbial community. The organic nutrients of seawater samples are analyzed using cutting-edge techniques to distinguish the types of nutrients that are processed by sponges. The incubation experiments, using free-living microbes collected from the coral reef, quantify the impact of sponge filtration on the growth and composition of this community. This project provides fundamental understanding of how sponges contribute to the base of the coral reef food web. As the human-driven impacts continue to alter the composition of organisms on reefs, this understanding is necessary to predict changes to reef microbial food webs and is thus essential for scientists, reef managers, and policy decision makers. This project trains undergraduate students and a postdoctoral scholar and contributes to undergraduate and K-12 education through development of sponge-centric lessons that focus on local U.S. east coast aquatic environments as well as coral reef ecosystems.</p> <p>Sponges vary in their capacity to filter seawater and in their associated microbial communities, leading to diverse metabolic strategies that often coexist in one habitat. While it is well-established that sponges are important in processing dissolved organic matter (DOM), an important reservoir of reduced carbon compounds, and transferring this energy to benthic food webs, there has been limited work to understand the consequences of sponge processing on the composition of coral reef DOM and on pelagic food webs. Specifically, while studies have shown that exudates of corals and algae select for specific groups of picoplankton (autotrophic and heterotrophic, respectively), similar data for sponges are required to understand the multiple factors that shape the composition of DOM and of the picoplankton community on coral reefs. Thus, this project is aimed at addressing a major knowledge gap of the role of sponge-derived DOM (sponge exometabolome) in coral reef biogeochemistry. An in situ sampling design targeting prominent Caribbean sponges and picoplankton incubation experiments is coupled to address both the composition of sponge exometabolomes and delineate shifts in the picoplankton community derived from sponge exometabolomes. Molecular-level changes to seawater DOM by sponge processing and the impact of these changes on the overall coral reef DOM profile is assessed with two DOM analysis techniques: a commonly used fluorometry technique (fDOM analysis) and with high-resolution mass spectrometry (LC-MS/MS). Additionally, microbiome and functional gene profiling, growth metrics, and nutrient analyses are employed to assess changes in the picoplankton community in response to sponge exometabolomes. Advanced data analysis techniques then synthesize data generated by each approach to provide novel insight on a poorly uncharacterized biogeochemical pathway on coral reefs. The work outlined here represents entirely novel information on the impact of sponge metabolism on the composition of DOM, sheds light on biologically important molecules involved in benthic-pelagic coupling, and importantly, generates data using standardized methods, thus facilitating comparison to previous and future DOM datasets.</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> Sponge Holobiont DOM largerWorkCitation project eng; USA oceans -81.40809 -81.40809 24.545344 24.545344 2022-01-07 2022-01-13 Caribbean Sea 0 BCO-DMO catalogue of parameters from Results of incurrent/excurrent water samples taken from three sponge species from the Florida Keys (USA) over two or three days using a modified version of the vacuSIP in January 2022 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/965591.rdf Name: Sponge_species Units: unitless Description: <p>Genus and species sample was taken from</p> http://lod.bco-dmo.org/id/dataset-parameter/965592.rdf Name: Replicate_number Units: unitless Description: <p>Number of sample taken for each species</p> http://lod.bco-dmo.org/id/dataset-parameter/965593.rdf Name: Water_type Units: unitless Description: <p>Type of water the sample was taken of: inhalant or exhalant</p> http://lod.bco-dmo.org/id/dataset-parameter/965594.rdf Name: Day Units: unitless Description: <p>Day of the survey</p> http://lod.bco-dmo.org/id/dataset-parameter/965595.rdf Name: Site Units: unitless Description: <p>Reef location for the survey</p> http://lod.bco-dmo.org/id/dataset-parameter/965596.rdf Name: Depth Units: meters Description: <p>Depth of sample locations</p> http://lod.bco-dmo.org/id/dataset-parameter/965597.rdf Name: Volume Units: milliliters (mL) Description: <p>Volume of inhalant or exhallant water collected</p> http://lod.bco-dmo.org/id/dataset-parameter/965598.rdf Name: Date Units: unitless Description: <p>Date of the survey</p> http://lod.bco-dmo.org/id/dataset-parameter/965599.rdf Name: Latitude Units: decimal degrees Description: <p>latitude of the reef site</p> http://lod.bco-dmo.org/id/dataset-parameter/965600.rdf Name: Longitude Units: decimal degrees Description: <p>longitude of the reef site</p> http://lod.bco-dmo.org/id/dataset-parameter/965601.rdf Name: Total_sample_RU_after_blank_subtraction Units: Raman units of water (RU) Description: <p>Total Raman units in the sample minus the Raman units of the milliQ water blank</p> http://lod.bco-dmo.org/id/dataset-parameter/965602.rdf Name: M_C_ratio Units: unitless Description: <p>M:C. Ratio of Coble Peak M (Marine Humic-like) to Cole Peak C (Visible Humic-like)</p> http://lod.bco-dmo.org/id/dataset-parameter/965603.rdf Name: BIX Units: biotic index score Description: <p>Biological Index meaning the tolerance values assigned to species.</p> http://lod.bco-dmo.org/id/dataset-parameter/965604.rdf Name: HIX Units: unitless Description: <p>Jumification index indicates the ratio between the humified and the non-humified portion of an organic substance</p> http://lod.bco-dmo.org/id/dataset-parameter/965605.rdf Name: FI Units: unitless Description: <p>Fluorescence Index used to distinguish a microbial (FI &gt; 1.8) or terrestrial (FI &lt; 1.2) source</p> http://lod.bco-dmo.org/id/dataset-parameter/965606.rdf Name: Ultra_Violet_Humic_like Units: Ramen Units (R.U.) Description: <p>Coble Peak A - Flourescent Dissolved Organic Matter (fDOM)</p> http://lod.bco-dmo.org/id/dataset-parameter/965607.rdf Name: Marine_Humic_like Units: Ramen Units (R.U.) Description: <p>Coble Peak M - Flourescent Dissolved Organic Matter (fDOM)</p> http://lod.bco-dmo.org/id/dataset-parameter/965608.rdf Name: Visible_Humic_like Units: Ramen Units (R.U.) Description: <p>Coble Peak C - Flourescent Dissolved Organic Matter (fDOM)</p> http://lod.bco-dmo.org/id/dataset-parameter/965609.rdf Name: Tryptophan_like Units: Ramen Units (R.U.) Description: <p>Coble Peak T - Flourescent Dissolved Organic Matter (fDOM)</p> http://lod.bco-dmo.org/id/dataset-parameter/965610.rdf Name: Tyrosine_like Units: Ramen Units (R.U.) Description: <p>Coble Peak B - Flourescent Dissolved Organic Matter (fDOM)</p> http://lod.bco-dmo.org/id/dataset-parameter/965611.rdf Name: Phenylalanine_like Units: Ramen Units (R.U.) Description: <p>Coble Peak F - Flourescent Dissolved Organic Matter (fDOM)</p> http://lod.bco-dmo.org/id/dataset-parameter/965612.rdf Name: Lignin_like Units: Raman units of water (RU) Description: <p>Peaks corresponding to lignin phenols as in Herenes et al. 2009</p> http://lod.bco-dmo.org/id/dataset-parameter/965613.rdf Name: TOC Units: various Description: <p>Total organic carbon</p> http://lod.bco-dmo.org/id/dataset-parameter/965614.rdf Name: TN Units: Micromolar (uM) Description: <p>Micromolar concentration of total nitrogen</p> http://lod.bco-dmo.org/id/dataset-parameter/965615.rdf Name: Phosphate Units: micromoles per liter (umol/l) Description: <p>Concentration of dissolved phosphate in micromoles per liter of sample water</p> http://lod.bco-dmo.org/id/dataset-parameter/965616.rdf Name: Nitrate_plus_Nitrite Units: micromoles per liter (umol/l) Description: <p>Concentration of dissolved inorganic (nitrate+nitrite) in micromoles per liter of sample water</p> http://lod.bco-dmo.org/id/dataset-parameter/965617.rdf Name: Silicate Units: micromoles per liter (umol/l) Description: <p>Concentration of dissolved silicate in micromoles per liter of sample water</p> http://lod.bco-dmo.org/id/dataset-parameter/965618.rdf Name: Nitrite Units: micromoles per liter (umol/l) Description: <p>Concentration of dissolved nitrite in micromoles per liter of sample water</p> http://lod.bco-dmo.org/id/dataset-parameter/965619.rdf Name: Ammonium Units: micromoles per liter (umol/l) Description: <p>Concentration of dissolved ammonium in micromoles per liter of sample water</p> http://lod.bco-dmo.org/id/dataset-parameter/965620.rdf Name: Prochlorococcus Units: cells per milliliter Description: <p>Prochlorococcus</p> http://lod.bco-dmo.org/id/dataset-parameter/965621.rdf Name: Synechococcus Units: cells per milliliter Description: <p>Synechococcus</p> http://lod.bco-dmo.org/id/dataset-parameter/965622.rdf Name: LowPE_Synechococcus Units: cells per milliliter Description: <p>Low Phycoerythrin Synechococcus</p> http://lod.bco-dmo.org/id/dataset-parameter/965623.rdf Name: Picoeukaryotes Units: cells per milliliter Description: <p>Photosynthetic eaukaryotes</p> http://lod.bco-dmo.org/id/dataset-parameter/965624.rdf Name: Heterotrophic_bacteria Units: cells per milliliter Description: <p>heterotrophic (non-pigmented) bacteria</p> http://lod.bco-dmo.org/id/dataset-parameter/965625.rdf Name: _2_3_dihydroxypropane_1_sulfonate Units: nanograms per liter (ng/l) Description: <p>2,3-dihydroxypropane-1-sulfonate. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965626.rdf Name: _2_3_dihydroxybenzoic_acid Units: nanograms per liter (ng/l) Description: <p>2,3-dihydroxybenzoic acid. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965627.rdf Name: _2prime_deoxycytidine Units: nanograms per liter (ng/l) Description: <p>2&#039;-deoxycytidine. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965628.rdf Name: _2_methyl_4_oxopentanoic Units: nanograms per liter (ng/l) Description: <p>2&#039;-deoxycytidine. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965629.rdf Name: _2_Keto_3_deoxy_6_phosphogluconate Units: nanograms per liter (ng/l) Description: <p>2-Keto-3-deoxy-6-phosphogluconate. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965630.rdf Name: _3_methyl_2_oxobutanoic_acid Units: nanograms per liter (ng/l) Description: <p>3-methyl-2-oxobutanoic acid. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965631.rdf Name: _4_aminobenzoic_acid Units: nanograms per liter (ng/l) Description: <p>4-aminobenzoic acid. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965632.rdf Name: _4_methyl_2_oxopentanoic_acid Units: nanograms per liter (ng/l) Description: <p>4-methyl-2-oxopentanoic acid. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965633.rdf Name: _6_hydroxymelatonin Units: nanograms per liter (ng/l) Description: <p>6-hydroxymelatonin. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965634.rdf Name: adenine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965635.rdf Name: adenosine_5prime_monophosphate Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965636.rdf Name: adenosine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965637.rdf Name: alanine_isom_sarcosine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965638.rdf Name: _4_amino_5_aminomethyl_2_methylpyrimidine Units: nanograms per liter (ng/l) Description: <p>4-amino-5-aminomethyl-2-methylpyrimidine. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965639.rdf Name: anthranilate Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965640.rdf Name: arginine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965641.rdf Name: argininosuccinic_acid Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965642.rdf Name: caffeine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965643.rdf Name: carnitine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965644.rdf Name: choline Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965645.rdf Name: ciliatine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965646.rdf Name: citric_acid Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965647.rdf Name: citrulline Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965648.rdf Name: cyanocobalamin Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965649.rdf Name: cysteine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965650.rdf Name: cytidine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965651.rdf Name: cytosine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965652.rdf Name: desthiobiotin Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965653.rdf Name: chitobiose Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965654.rdf Name: dimethylsulfoniopropionate Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965655.rdf Name: D_fructose_1_6_bisphosphate Units: nanograms per liter (ng/l) Description: <p>D-fructose 1,6-bisphosphate. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965656.rdf Name: fructose_6_phosphate Units: nanograms per liter (ng/l) Description: <p>fructose 6-phosphate. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965657.rdf Name: fumaric_acid Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965658.rdf Name: gamma_aminobutyric_acid Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965659.rdf Name: D_glucosamine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965660.rdf Name: glucose_6_phosphate Units: nanograms per liter (ng/l) Description: <p>glucose 6-phosphate. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965661.rdf Name: glutamic_acid Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965662.rdf Name: glutamine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965663.rdf Name: glutathione Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965664.rdf Name: sn_glycerol_3_phosphate Units: nanograms per liter (ng/l) Description: <p>sn-glycerol 3-phosphate. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965665.rdf Name: glycine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965666.rdf Name: glycine_betaine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965667.rdf Name: guanine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965668.rdf Name: guanosine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965669.rdf Name: _4_methyl_5_thiazoleethanol Units: nanograms per liter (ng/l) Description: <p>4-methyl-5-thiazoleethanol. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965670.rdf Name: histadine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965671.rdf Name: histidinol Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965672.rdf Name: homocysteine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965673.rdf Name: hydroxocobalamin Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965674.rdf Name: hydroxyproline Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965675.rdf Name: hypoxanthine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965676.rdf Name: inosine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965677.rdf Name: inosine_5prime_monophosphate Units: nanograms per liter (ng/l) Description: <p>inosine 5&#039;-monophosphate. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965678.rdf Name: isoleucine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965679.rdf Name: kynurenine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965680.rdf Name: leucine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965681.rdf Name: lumichrome Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965682.rdf Name: malic_acid Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965683.rdf Name: methionine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965684.rdf Name: methylmalonic_acid Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965685.rdf Name: _5prime_methylthioadenosine Units: nanograms per liter (ng/l) Description: <p>5&#039;-methylthioadenosine. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965686.rdf Name: n_acetyl_glucosamine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965687.rdf Name: N_acetyl_D_glucosamine_6_phosphate Units: nanograms per liter (ng/l) Description: <p>N-acetyl-D-glucosamine 6-phosphate. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965688.rdf Name: n_acetyl_glutamic_acid Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965689.rdf Name: n_acetyl_muramic_acid Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965690.rdf Name: phosphoserine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965691.rdf Name: ornithine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965692.rdf Name: pantothenic_acid Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965693.rdf Name: phenylalanine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965694.rdf Name: proline Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965695.rdf Name: riboflavin Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965696.rdf Name: D_Ribose_5_phosphate Units: nanograms per liter (ng/l) Description: <p>D-Ribose 5-phosphate. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965697.rdf Name: serine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965698.rdf Name: taurocholic_acid Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965699.rdf Name: spermidine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965700.rdf Name: succinic_acid Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965701.rdf Name: sucrose Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965702.rdf Name: syringic_acid Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965703.rdf Name: thiamine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965704.rdf Name: threonine_homoserine Units: nanograms per liter (ng/l) Description: <p>threonine / homoserine. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965705.rdf Name: thymidine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965706.rdf Name: chitotriose Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965707.rdf Name: tryptophan Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965708.rdf Name: tyrosine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965709.rdf Name: uric_acid Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965710.rdf Name: uridine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965711.rdf Name: uridine_5prime_monophosphate Units: nanograms per liter (ng/l) Description: <p>uridine 5&#039;-monophosphate. Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965712.rdf Name: valine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965713.rdf Name: xanthine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/965714.rdf Name: xanthosine Units: nanograms per liter (ng/l) Description: <p>Dissolved organic metabolite; Concentration in nanograms per liter of sample</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 24141 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/46204335-6147-4190-99d2-6f8490826bde/content download https://doi.org/10.26008/1912/bco-dmo.965557.1 download onLine dataset &lt;p&gt;Incurrent/excurrent water samples were taken from three sponge species from the Florida Keys (USA) over two or three days using a modified version of the vacuSIP (Morganti et al. 2016). Briefly, acid-cleaned and combusted bottles were negatively pressurized and sealed. PEEK tubing lines were connected to the pressurized bottles via a needle, and this pulled seawater from the opening of the sponge (&quot;excurrent&quot;) or nearby seawater (&quot;incurrent&quot;) at a slow and steady rate to fill the bottles. Nutrient data from the seawater samples were compiled and organized by sponge species and water type (incurrent or excurrent) in an Excel file. Seawater samples from two sponge species (&lt;em&gt;Niphates digitalis&lt;/em&gt; and &lt;em&gt;Verongula rigida&lt;/em&gt;) were filtered through 0.2-micrometer (µm) polyethersulfone filters, while seawater from the sponge &lt;em&gt;Xestspongia muta&lt;/em&gt; was filtered through 0.2 µm Teflon Omnipore filters. Filtrate was then saved for inorganic nutrients and those from &lt;em&gt;X. muta&lt;/em&gt; were processed for dissolved organic matter composition using targeted metabolomics analysis. Flow cytometry of phytoplankton and bacteria data was collected from pre-filtered seawater and preserved in 0.5% paraformaldehyde final concentration. Seawater for total organic carbon (TOC) and total nitrogen (TN) was also collected prior to filtration and was acidified to ~pH 2 using concentrated HCl. Metabolomics and TOC analysis were performed at Woods Hole Oceanographic Institution at the Mass Spectrometry Facility.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Dissolved Organic Matter Extraction:&lt;/strong&gt;&lt;br /&gt; Filtered seawater was processed using PPL solid phase extraction following the protocol by Dittmar et al. (2008). Extracts were dried to nearly completeness, leaving a small viscous drop in the vial. These extracts were then shipped to WHOI for metabolomics analysis.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Targeted Metabolite Analysis by UPLC-MS:&lt;/strong&gt;&lt;br /&gt; DOM extracts were reconstituted in 200 microliters (μl) MilliQ water with 50 nanograms per milliliter (ng/ml) isotopically-labeled injection standards d2 biotin, d6 succinic acid, d4 cholic acid, and d7 indole 3 acetic acid. We used ultra-performance liquid chromatography (Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific) coupled to a heated electrospray ionization source (H-ESI) and a triple quadrupole mass spectrometer (TSQ Vantage, Thermo Scientific) operated under selected reaction monitoring (SRM) mode. We performed chromatographic separation with a Waters Acquity HSS T3 column (2.1 × 100 millimeters (mm), 1.8 μm) equipped with a Vanguard pre-column and maintained at 40 degrees Celsius (°C). We eluted the metabolites from the column with (A) 0.1% formic acid in water and (B) 0.1% formic acid in acetonitrile at a flow rate of 0.5 milliliters per minute (mL min-1), according to the gradient: 0 min, 1% B; 1 min, 1%B; 3 min, 15%B; 6 min, 50%B; 9 min, 95%B; 10 min, 95%B; 10.2 min, 1%B; 12 min, 1%B (total run time = 12 min). Settings for source gases were 55 (sheath), 20 (auxiliary) and 0 (sweep), and these settings are presented in arbitrary units. The heated capillary temperature was 375 °C and the vaporizer temperature was 400 °C. For positive and negative modes, we performed separate autosampler injections of 5 μL each.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Flow Cytometry:&lt;/strong&gt;&lt;br /&gt; Samples were preserved and stored at -80°C until later batch analysis at the University of Hawaii SOEST Flow Cytometry Facility (&lt;a href=&quot;http://www.soest.hawaii.edu/sfcf&quot; target=&quot;_blank&quot;&gt;www.soest.hawaii.edu/sfcf&lt;/a&gt;). Microbial cells were enumerated using flow cytometry (Selph, 2021). In brief, samples (0.1 mL) were thawed in batches, stained with the DNA dye Hoechst 34580 (1 microgram per milliliter (µg/mL) final), then run at 30 microliters per minute (µL min-1) on a Beckman-Coulter CytoFlex S flow cytometer, using lasers emitting at 375 nanometers (nm) (to detect Hoechst), 488 nm (for scatter and chlorophyll parameters), and 561 nm (for phycoerythrin). Resulting listmode files (FCS 3.0) were analyzed using FlowJo software (Becton Dickinson, v. 10.8.2) to distinguish microbial populations based on their fluorescence signals (chlorophyll, phycoerythrin, DNA), as well as forward and right-angle light scatter. Heterotrophic bacteria were distinguished from phytoplankton by their DNA signature and absence of pigment. &lt;em&gt;Prochlorococcus&lt;/em&gt; and &lt;em&gt;Synechococcus&lt;/em&gt; were separated from larger eukaryotic phytoplankton by their light scatter signatures, as well as their characteristic pigment and DNA signatures. Other phytoplankton (eukaryotes, mostly 2-20 µm pico- and nano-sized cells given the small volume analyzed) had higher light scatter and more chlorophyll fluorescence per cell.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Inorganic nutrients:&lt;/strong&gt;&lt;br /&gt; Inorganic nutrients included phosphate, nitrate+nitrite, nitrite, ammonia, and silicic acid. The phosphate method is a modification of the molybdenum blue procedure of Bernhardt and Wilhelms (1967), in which phosphate is determined as reduced phosphomolybdic acid employing hydrazine as the reductant. The nitrate + nitrite analysis uses the basic method of Armstrong et al. (1967), with modifications to improve the precision and ease of operation. Sulfanilamide and N-(1-Napthyl)ethylenediamine dihydrochloride react with nitrite to form a colored diazo compound. For the nitrate + nitrite analysis, nitrate is first reduced to nitrite using an OTCR and imidazole buffer as described by Patton (1983). Nitrite analysis is performed on a separate channel, omitting the cadmium reductor and the buffer. The method is based on that of Armstrong et al. (1967) as adapted by Atlas et al. (1971). The addition of an acidic molybdate reagent forms silicomolybdic acid, which is then reduced by stannous chloride. This indophenol blue method is modified from ALPKEM RFA methodology which references Methods for Chemical Analysis of Water and Wastes, March 1984, EPA-600/4-79-020, &quot;Nitrogen Ammonia&quot;, Method 350.1 (Colorimetric, Automated Phenate) A detailed description of the continuous segmented flow procedures used can be found in Gordon et. al. (1994).&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;&lt;strong&gt;Targeted Metabolomics:&lt;/strong&gt;&lt;br /&gt; Samples were analyzed in random order and injected pooled samples at regular intervals (every 8 samples). We monitored two SRM transitions per compound for quantification and confirmation; these transitions were optimized previously using authentic standards. We generated 8-point external calibration curves based on peak area for each compound. We converted raw data files from proprietary Thermo (.RAW) format to mzML using the msConvert tool (Chambers et al., 2012) prior to processing with El-MAVEN (Agrawal et al., 2019). Metabolite concentrations were provided from WHOI as raw data in nanograms per milliliter (ng/ml) in the 200 microliters (μl) extract. We then converted the ng/ml values into total nanograms by multiplying by 0.2 then divided by the sample volume in liters to produce nanograms per liter (ng/L) concentrations. These concentrations were converted to micromolar concentrations using the formula weight for each metabolite.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Statistical Analysis:&lt;/strong&gt;&lt;br /&gt; Concentrations of dissolved nutrients (micromolar (μM)) and cells (cells per milliliter) were compared by analysis of variance (ANOVA) or Kruskal-Wallis, depending on if the dataset met assumption of ANOVA, followed by pairwise comparisons (either Tukey HSD or Dunn test). Permutational analysis of variance (PERMANOVA) was also used to assess an effect of sample, sponge species, or day on a matrix of all nutrients following conversion of the data to z-score.&lt;/p&gt; Specified by the Principal Investigator(s) - Imported submitter's file named &quot;965557_v1_sponge_3day_vacusip_jan2022_CFmod082025.csv&quot; into the BCO-DMO system. - Renamed the &quot;_aminobutyric_acid&quot; column to &quot;gamma_aminobutyric_acid&quot;. - Converted Date field to YYYY-MM-DD format. - Saved the final file as &quot;965557_v1_sponge_3day_vacusip_jan2022.csv&quot;. Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Alpkem RFA 300 Alpkem RFA 300 PI Supplied Instrument Name: Alpkem RFA 300 PI Supplied Instrument Description:Inorganic nutrients measured using Technicon AutoAnalyzer II™ components were used to measure phosphate and ammonium; and Alpkem RFA 300™ components were used for silicic acid, nitrate plus nitrite, and nitrite. Instrument Name: Alpkem RFA300 Instrument Short Name:Alpkem RFA300 Instrument Description: A rapid flow analyser (RFA) that may be used to measure nutrient concentrations in seawater. It is an air-segmented, continuous flow instrument comprising a sampler, a peristaltic pump which simultaneously pumps samples, reagents and air bubbles through the system, analytical cartridge, heating bath, colorimeter, data station, and printer. The RFA-300 was a precursor to the smaller Alpkem RFA/2 (also RFA II or RFA-2). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0414/ Beckman-Coulter CytoFLEX S flow cytometer Beckman-Coulter CytoFLEX S flow cytometer PI Supplied Instrument Name: Beckman-Coulter CytoFLEX S flow cytometer PI Supplied Instrument Description:A Beckman-Coulter CytoFLEX S flow cytometer was used to analyze all samples. This instrument is equipped with four lasers emitting light in spatially separated pathways at 375 nm (60 mW), 405 nm (80 mW), 488 nm (50 mW), and 561 nm (30 mW). Acquisition software was CytExpert (v. 2.3.1.22). Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/ Technicon AutoAnalyzer II Technicon AutoAnalyzer II PI Supplied Instrument Name: Technicon AutoAnalyzer II PI Supplied Instrument Description:Inorganic nutrients measured using Technicon AutoAnalyzer II™ components were used to measure phosphate and ammonium; and Alpkem RFA 300™ components were used for silicic acid, nitrate plus nitrite, and nitrite. Instrument Name: Technicon AutoAnalyzer II Instrument Short Name: Instrument Description: A rapid flow analyzer that may be used to measure nutrient concentrations in seawater. It is a continuous segmented flow instrument consisting of a sampler, peristaltic pump, analytical cartridge, heating bath, and colorimeter. See more information about this instrument from the manufacturer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0291/ ultra-performance liquid chromatography (Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific) ultra-performance liquid chromatography (Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific) PI Supplied Instrument Name: ultra-performance liquid chromatography (Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific) PI Supplied Instrument Description:Targeted metabolomics were measured using the ultra-performance liquid chromatography (Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific) coupled to a heated electrospray ionization source (H-ESI) and a triple quadrupole mass spectrometer (TSQ Vantage, Thermo Scientific) operated under selected reaction monitoring (SRM) mode  at the Woods Hole Oceanographic Institution (WHOI).  Instrument Name: Ultra-high-performance liquid chromatography Instrument Short Name:UHPLC Instrument Description: Ultra high-performance liquid chromatography: Column chromatography where the mobile phase is a liquid, the stationary phase consists of very small (< 2 microm) particles and the inlet pressure is relatively high.