http://lod.bco-dmo.org/id/dataset/966932
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-07-07
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Larvae from Seafloor Larval Observatory (SLO) benthic traps with RNALater deployed during cruise TN391 (Jun 2021) and recovered on cruise AT50-04 (Oct 2022) in the Gulf of Mexico and Western Atlantic
2025-07-07
publication
2025-07-07
revision
BCO-DMO Linked Data URI
2025-07-07
creation
http://lod.bco-dmo.org/id/dataset/966932
Shawn M. Arellano
Western Washington University - Shannon Point Marine Center
principalInvestigator
David B. Eggleston
North Carolina State University
principalInvestigator
Craig M. Young
University of Oregon
principalInvestigator
Ruoying He
North Carolina State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Eggleston, D. B., Young, C. M., Arellano, S. M., He, R. (2025) Larvae from Seafloor Larval Observatory (SLO) benthic traps with RNALater deployed during cruise TN391 (Jun 2021) and recovered on cruise AT50-04 (Oct 2022) in the Gulf of Mexico and Western Atlantic. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-07-07 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/966932 [access date]
Dataset Description: <p>SLO metadata tablea are included as supplemental tables in this dataset (See "Files" section).<br />
* See "Related Datasets" section for other logs and sample lists from this cruise.<br />
* Data from this and other cruises in this project are listed under the SALT project page https://www.bco-dmo.org/project/820030.</p>
<p>SALT = Seep Animal Larval Transport.<br />
SLO =&nbsp;Seafloor Larval Observatory</p> Methods and Sampling: <p>Larvae were passively collected via benthic tube traps filled with RNALater. Some samples are from Technicap carousels that only had one tube collecting at a time and a motor that rotated sample tubes each month for 12 discrete monthly samples. Others were from homemade PVC larval traps that collected integrated samples. Traps were deployed in June 2021 on TN391 and recovered in October 2022 on AT50-04. Once recovered, sample tubes were capped and stored in a refrigerator (~4 °C) for months to a year before being processed. Samples were examined under dissecting microscopes and individual larvae picked out by hand by a team of trained graduate and undergraduate students. Isolated larvae were photographed under a compound microscope, individually tubed in fresh RNALater, and shipped to Shawn Arellano's lab at Western Washington University for genetic identification.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1851383 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1851383
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1851286 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1851286
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1851421 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1851421
onGoing
Shawn M. Arellano
Western Washington University - Shannon Point Marine Center
360-650-3634
Western Washington University 1900 Shannon Point Road
Anacortes
WA
98221
USA
arellas@wwu.edu
pointOfContact
David B. Eggleston
North Carolina State University
919-515-7840
32800 Faucette Drive PO Box 8208
Raleigh
NC
27695
USA
eggleston@ncsu.edu
pointOfContact
Craig M. Young
University of Oregon
541-346-7274
Oregon Institute of Marine Biology P.O. Box 5389
Charleston
Oregon
97420
USA
cmyoung@uoregon.edu
pointOfContact
Ruoying He
North Carolina State University
919-513-0249
Jordan Hall - Rm 4149
Raleigh
NC
27695-8208
USA
rhe@ncsu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample_Method
Equipment_Num
Tube_Num
RecoveryDive
Site
Sample_ID
Form_Morphotype_ID
Photo_ID
Notes
Num_Individuals_in_tube
Fixation_Method_in_tube
Box_Num
Current_Location
tube trap
theme
None, User defined
sample description
Sensor/Instrument identifier
dive_id
site
sample identification
animal group (category)
file_name
comments
count
featureType
BCO-DMO Standard Parameters
no_bcodmo_term
instrument
BCO-DMO Standard Instruments
AT50-04
TN391
service
Deployment Activity
Western Atlantic margin and Gulf of Mexico from Pensacola, FL to Woods Hole, MA
Woods Hole, Massachusetts to Gulfport, Mississippi
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: dispersal depth and the transport of deep-sea, methane-seep larvae around a biogeographic barrier
https://wp.wwu.edu/arellanolab/category/salt/
Collaborative Research: dispersal depth and the transport of deep-sea, methane-seep larvae around a biogeographic barrier
<p><em>NSF Award Abstract:</em><br />
Ever since hydrothermal vents and methane seeps were first discovered in the deep ocean more than 40 years ago, scientists have wondered how these isolated communities, fully dependent on underwater "islands" of toxic chemicals, are first colonized by organisms, and how the populations of these specialized animals are exchanged and maintained. These fundamental processes depend on the transport of babies (larvae) by the ocean currents, yet because the larvae are microscopic and diluted in the vastness of the ocean, it is very difficult to determine where and how they drift. This project uses an autonomous underwater vehicle to collect larvae from precise regions of the water column. Larval traps on the bottom and chemical analyses of larval shells will also be used to determine the depths where larvae swim. These findings will provide realistic estimates for mathematical models that show how biology interacts with ocean currents to predict which methane seeps will be colonized by larvae originating at different depths. A detailed knowledge of larval dispersal is needed for conservation and management of the deep sea. Without such information, we cannot know the best placement of marine protected areas, nor can we facilitate the reestablishment of communities impacted by deep-sea mining, drilling, or other human activities. This project will provide hands-on at-sea training for college students to learn the rapidly vanishing skills needed for studies of larvae and embryos in their natural habitats. Learning opportunities will also be available to individuals of all ages through new, interactive exhibits on deep-sea biology and larval ecology produced for small museums and aquaria on the coasts of Oregon, Washington and North Carolina.</p>
<p>Reliable estimates of connectivity among metapopulations are increasingly important in marine conservation biology, ecology and phylogeography, yet biological parameters for biophysical models in the deep sea remain largely unavailable. The movements of deep-sea vent and seep larvae among islands of habitat suitable for chemosynthesis have been inferred from current patterns using numerical modeling, but virtually all such models have used untested assumptions about biological parameters that should have large impacts on the predictions. This project seeks to fill in the missing biological parameters while developing better models for predicting the dispersal patterns of methane seep animals living in the Gulf of Mexico and on the Western Atlantic Margin. Despite the existence of similar seeps at similar depths on two sides of the Florida peninsula, the Western Atlantic seeps support only a subset of the species found in the Gulf of Mexico. It is hypothesized that the ability of larvae to disperse through the relatively shallow waters of the Florida Straits depends on an interaction between the adult spawning depth and the dispersal depth of the larvae. Dispersal depth, in turn, will be influenced by larval flotation rates, swimming behaviors, feeding requirements, and ontogenetic migration patterns during the planktonic period. The recently developed SyPRID sampler deployed on AUV Sentry will be used to collect larvae from precise depth strata in the water column, including layers very near the ocean floor. Larval traps deployed on the bottom at three depths in each region will be used in conjunction with the plankton collections to determine what proportion of larvae are demersal. Comparisons of stable oxygen isotopes between larval and juvenile mollusk shells will provide information on the temperatures (and therefore depths) that larvae develop, and geochemical analyses of larval and juvenile shells will determine whether larval cohorts mix among depth strata. Ocean circulation and particle transport modeling incorporating realistic biological parameters will be used to predict the movements of larvae around the Florida Peninsula for various spawning depths and seasons.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
SALT
largerWorkCitation
project
eng; USA
oceans
Western Atlantic margin and Gulf of Mexico from Pensacola, FL to Woods Hole, MA; Woods Hole, Massachusetts to Gulfport, Mississippi
2022-10-13
2022-11-01
Methane seeps on the shelf and slope of Louisiana, Mississippi, Florida, North Carolina, Virginia and Maryland
0
BCO-DMO catalogue of parameters from Larvae from Seafloor Larval Observatory (SLO) benthic traps with RNALater deployed during cruise TN391 (Jun 2021) and recovered on cruise AT50-04 (Oct 2022) in the Gulf of Mexico and Western Atlantic
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/967872.rdf
Name: Sample_Method
Units: unitless
Description: <p>The type of benthic trap used, either a Technicap carousel with discrete monthly samples or a homemade PVC tube trap with integrated samples.</p>
http://lod.bco-dmo.org/id/dataset-parameter/967873.rdf
Name: Equipment_Num
Units: unitless
Description: <p>Equiptment number. Allows one to match the specific trap and thus the deployment location information using the AT50-04 Master Sample List.</p>
http://lod.bco-dmo.org/id/dataset-parameter/967874.rdf
Name: Tube_Num
Units: unitless
Description: <p>Refers to the individual sample tube. For Technicaps, this allows the samples to be matched to a specific month. For the PVC larval traps, there are two replicate tubes per trap. </p>
http://lod.bco-dmo.org/id/dataset-parameter/967875.rdf
Name: RecoveryDive
Units: unitless
Description: <p>Recovery Dive lists the Alvin (AL####) dive on which the equipment and samples were recovered.</p>
http://lod.bco-dmo.org/id/dataset-parameter/967876.rdf
Name: Site
Units: unitless
Description: <p>Site lists the name of the seep site at which the equipment was deployed.</p>
http://lod.bco-dmo.org/id/dataset-parameter/967877.rdf
Name: Sample_ID
Units: unitless
Description: <p>Sample ID is an internal code to keep track of sample throughout the various processing steps. It follows the format of: CruiseID_Dive_EquipmentType_LarvalMorphotypeCode_SampleNumber</p>
http://lod.bco-dmo.org/id/dataset-parameter/967878.rdf
Name: Form_Morphotype_ID
Units: unitless
Description: <p>Identifier (internal) for morphotype that starts with a two-letter code and sometimes includes a specific number [keys to the two-letter codes are in the Larval Morphotype Key table (see supplemental files)]. </p>
http://lod.bco-dmo.org/id/dataset-parameter/967879.rdf
Name: Photo_ID
Units: unitless
Description: <p>Photo_ID Lists the file name(s) for any associated photo(s). It is the same as the sample ID but includes the magnification at which the photo was taken. </p>
http://lod.bco-dmo.org/id/dataset-parameter/967880.rdf
Name: Notes
Units: unitless
Description: <p>Notes has any applicable notes recorded during sample processing.</p>
http://lod.bco-dmo.org/id/dataset-parameter/967881.rdf
Name: Num_Individuals_in_tube
Units: unitless
Description: <p>Lists how many individual larvae (of the same morphotype) were put into each new sample tube.</p>
http://lod.bco-dmo.org/id/dataset-parameter/967882.rdf
Name: Fixation_Method_in_tube
Units: unitless
Description: <p>Lists the fresh chemical in the tubes of newly isolated larvae.</p>
http://lod.bco-dmo.org/id/dataset-parameter/967883.rdf
Name: Box_Num
Units: unitless
Description: <p>Box number identifies which cryovial box houses that sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/967884.rdf
Name: Current_Location
Units: unitless
Description: <p>Curent Location shows that all samples have been sent to Shawn Areallano's lab for genetic processing. </p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/966932/data/download
download
onLine
dataset
<p>Larvae were passively collected via benthic tube traps filled with RNALater. Some samples are from Technicap carousels that only had one tube collecting at a time and a motor that rotated sample tubes each month for 12 discrete monthly samples. Others were from homemade PVC larval traps that collected integrated samples. Traps were deployed in June 2021 on TN391 and recovered in October 2022 on AT50-04. Once recovered, sample tubes were capped and stored in a refrigerator (~4 °C) for months to a year before being processed. Samples were examined under dissecting microscopes and individual larvae picked out by hand by a team of trained graduate and undergraduate students. Isolated larvae were photographed under a compound microscope, individually tubed in fresh RNALater, and shipped to Shawn Arellano's lab at Western Washington University for genetic identification.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Samples were examined under dissecting microscopes and individual larvae picked out by hand by a team of trained graduate and undergraduate students. Isolated larvae were photographed under a compound microscope, individually tubed in fresh RNALater, and shipped to Shawn Arellano's lab at Western Washington University for genetic identification.<br />
<br />
Additional metadata to accompany data table:</p>
<div>Data compiled on: 11-Jun-25&nbsp;by: C.Q. Plowman</div>
<div>&nbsp;</div>
<div>Description: Larval tube traps deployed as part of the Seafloor Larval Observatories</div>
<div>1) Technicap Carousels - 12 tubes of RNALater and a motor that moves to a new tube each month &lt;- These data</div>
<div>2) PVC Larval Traps - two traps, each with 4 tubes of fixative deployed at each site</div>
<div>2 filled with RNALater to aid in genetic identification of larvae &lt;- These data</div>
<div>2 filled with 10% buffered formalin to aid in morphological identification of larvae &lt;- Sorting still in progress</div>
<div>These data are from traps deployed on TN391 and recovered on AT50-04</div>
<div>Sorting occurred in the Young Lab at the Oregon Institute of Marine Biology by a team of graduate and undergraduate students</div>
Specified by the Principal Investigator(s)
* Sheet "Data" of submitted file "AT50-04_Larval Sorting_tube traps_RNALater_11Jun25.xlsx" was imported into the BCO-DMO data system for this dataset. Values "NA" and "n/a" imported as missing data values. Table will appear as Data File: *.csv (along with other download format options).
Missing Data Identifiers:
* In the BCO-DMO data system missing data identifiers are displayed according to the format of data you access. For example, in csv files it will be blank (null) values. In Matlab .mat files it will be NaN values. When viewing data online at BCO-DMO, the missing value will be shown as blank (null) values.
* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores. Can not start with a number]
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
tube trap
tube trap
PI Supplied Instrument Name: tube trap PI Supplied Instrument Description:Technicap brand 12-tube carousel and motor in a custom plastic housing.
Homemade PVC larval tube traps. Instrument Name: no_bcodmo_term Instrument Short Name: Instrument Description: No relevant match in BCO-DMO instrument vocabulary. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/999/
Cruise: AT50-04
AT50-04
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT50-04
Craig M. Young
University of Oregon
Cruise: TN391
TN391
R/V Thomas G. Thompson
Community Standard Description
International Council for the Exploration of the Sea
R/V Thomas G. Thompson
vessel
TN391
Craig M. Young
University of Oregon
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
R/V Thomas G. Thompson
Community Standard Description
International Council for the Exploration of the Sea
R/V Thomas G. Thompson
vessel