http://lod.bco-dmo.org/id/dataset/967907 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2025-07-11 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Stable isotope analyses, mercury measurements, and capture data from Baltic fishes collected 2019-2021 for Project Breathless 2025-07-11 publication 2025-07-11 revision BCO-DMO Linked Data URI 2025-07-11 creation http://lod.bco-dmo.org/id/dataset/967907 Karin Limburg State University of New York College of Environmental Science and Forestry principalInvestigator Roxanne Razavi State University of New York College of Environmental Science and Forestry principalInvestigator Yvette Heimbrand Swedish University of Agricultural Sciences principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Limburg, K., Heimbrand, Y., Razavi, R., Miraly, H. (2025) Stable isotope analyses, mercury measurements, and capture data from Baltic fishes collected 2019-2021 for Project Breathless. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-07-11 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/967907 [access date] Baltic fishes SIA and Hg Dataset Description: &lt;p&gt;This dataset is part of Project Breathless, which brings together an interdisciplinary team of ecologists, economists and communication scholars to increase understanding of the impact of low oxygen dead zones in lakes and oceans.&amp;nbsp; The project team focuses on how low oxygen conditions influence fish, their habitats and the food webs that support them, as well as ecosystem services, including fisheries production.&lt;/p&gt; &lt;p&gt;This dataset is one of three examining Baltic fishes using data collected during routine surveys for monitoring the status of fish stocks:&lt;/p&gt; &lt;ul&gt; &lt;li&gt;&lt;strong&gt;Dataset 967907 (this dataset of stables isotopes and mercury) &lt;/strong&gt;summarizes capture data as well as basic biological information; it also includes all stable isotopic ratio and total mercury data collected from muscle tissue (though not from every fish).&amp;nbsp;&lt;/li&gt; &lt;li&gt;Dataset 967925 (Baltic fishes otolith chemistry)&amp;nbsp;contains all of the Baltic Sea fish otolith chemistry data; it also includes data from 14 Icelandic cod otoliths that were used as an out-group for part of the study&lt;/li&gt; &lt;li&gt;Dataset 967934 (Baltic fishes eye lens chemistry)&amp;nbsp;contains all of the eye lens chemistry data.&lt;/li&gt; &lt;/ul&gt; &lt;p&gt;&lt;em&gt;(See Related Datasets section below for links)&lt;/em&gt;&lt;/p&gt; Methods and Sampling: &lt;p&gt;Fish were collected during routine surveys performed for monitoring the status of fish stocks, either by national fishery management agencies (Department of Aquatic Resources, Swedish University of Agricultural Sciences, Icelandic Marine and Freshwater Research Institute) or international collaborative effort (Baltic International Trawl Survey). Fish were caught by trawl or by gillnet sets, then frozen for later processing.&lt;/p&gt; &lt;p&gt;In the lab, fish were measured for length and weighed. Sex and spawning stage were determined where possible. Otoliths and eye lenses were removed, cleaned, and stored until workup (see Related Datasets section below). Samples of dorsal muscle tissue were collected for mercury and stable isotope analysis and were dried to constant weight.&lt;/p&gt; &lt;p&gt;Total mercury analyses were conducted using atomic absorption spectrophotometry on a Milestone DMA-80 tri-cell unit (Milestone Srl, Italy). Briefly, the method involves thermal decomposition of a sample, amalgamation of mercury, and absorbance of mercury using a spectrophotometer.&amp;nbsp;Quality assurance and quality control was following EPA Method 7473, including daily calibration checks, the use of blanks, certified reference materials, and matrix spike/matrix spike duplicates. Sample duplicates were analyzed on a minimum of 20% of samples.&lt;/p&gt; &lt;p&gt;Stable isotopic ratio analyses for carbon (C), nitrogen (N), and sometimes sulfur (S)&amp;nbsp;were conducted by either the Stable Isotope Facility at the University of California at Davis or at the Cornell University Isotope Laboratory. Both facilities routinely analyze solid samples, such as ours, using elemental analyzers on the front end of isotope ratio mass spectrometers (EA-IRMS). Delta values are expressed relative to Vienna Pee Dee Belemnite (for carbon), air (for nitrogen), and Vienna Canyon Diablo Troilite (for sulfur) standards. Light stable isotopes of C and N were analyzed at UC Davis with&amp;nbsp;PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK). At Cornell, a Thermo Delta V isotope ratio mass spectrometer (IRMS) is interfaced to a NC2500 elemental analyzer (EA). Sulfur isotopes were analyzed separately on the same mass spectrometer at Cornell.&lt;/p&gt; &lt;p&gt;&lt;em&gt;(For otolith chemistry and fish eye lens chemistry results, see Related Datasets section below.)&lt;/em&gt;&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1923965 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1923965 onGoing Karin Limburg State University of New York College of Environmental Science and Forestry 315-470-6741 249 Illick Hall 1 Forestry Dr. Syracuse NY 13210 United States klimburg@esf.edu pointOfContact Roxanne Razavi State University of New York College of Environmental Science and Forestry razavi@esf.edu pointOfContact Yvette Heimbrand Swedish University of Agricultural Sciences yvette.heimbrand@slu.se pointOfContact asNeeded Dataset Version: 1 Unknown Fish_ID Species Year Date Survey Status Latitude Longitude Tot_Length Weight SLU_Age_years KL_Age_years Sex d13C_VPDB d15N_Air d34S_VCDT Tot_Hg_dry_weight Eye_Lens_Tot_Hg Oto_Analyzed Comments NC2500 Elemental Analyzer Milestone DMA-80 tri-cell unit atomic absorption spectrophotometer PDZ Europa 20-20 isotope ratio mass spectrometer  PDZ Europa ANCA-GSL elemental analyzer Thermo Delta V isotope ratio mass spectrometer (IRMS) theme None, User defined sample identification species scientific name (binomial) year date site site description latitude longitude fish_len weight age sex d13C measured in biota d15N measured in biota d34S_bio total mercury sample description comments featureType BCO-DMO Standard Parameters Carlo Erba NC 2500 elemental analyzer Milestone Direct Mercury Analyzer PDZ Europa 20-20 isotope ratio mass spectrometer PDZ Europa ANCA-GSL elemental analyzer Thermo Fisher Scientific DELTA V Advantage isotope ratio mass spectrometer instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Shifting the Hypoxia Paradigm – New Directions to Explore the Spread and Impacts of Ocean/Great Lakes Deoxygenation https://osprey.bco-dmo.org/project/770460 Collaborative Research: Shifting the Hypoxia Paradigm – New Directions to Explore the Spread and Impacts of Ocean/Great Lakes Deoxygenation <p><em>NSF Award Abstract:</em><br /> Ocean oxygen loss (deoxygenation) is increasing due to climate warming. This warming, together with nutrient loading, is causing many marine and freshwater systems to experience increasing episodes of hypoxia (low oxygen) of greater duration and intensity. Impacts on fish and fisheries have been difficult to quantify; direct observation has been challenged by a lack of long-term exposure indicators. This team has successfully refined the use of fish chemical biomarkers in fish otoliths (earstones) to directly assess lifetime hypoxia exposure in fishes. This project will those findings to look for additional biomarkers and models, to expand our understanding of how hypoxia affects fish and their food webs, contaminant transfers, and ecosystem services including economic impacts. The project includes a unique way of training students in science communication, posing the question: What forms of media and "messaging strategies" about deoxygenation are most effective at raising public awareness and understanding? Students are developing entries for PlanetForward's Storyfest, which is a contest to tell compelling stories to foster environmental understanding and solutions. Students from historically underrepresented, economically disadvantaged backgrounds are particularly sought out to participate. The investigators will engage with regional, national, and international management agencies and other relevant stakeholder groups to share information.</p> <p>This project encompasses a novel, linked set of interdisciplinary studies of food webs, and ecosystem services assessment. The thematic questions explored in this project are: 1. How does hypoxia alter habitat use for fishes? 2. How does hypoxia-altered habitat use and habitat productivity change food webs? 3. How does hypoxia affect/enhance trophic transfer of methylmercury? 4. How do hypoxia-induced changes in food webs affect aquatic ecosystem services? The set of linked studies will employ chemical analyses of otoliths and eye lenses, combined with chemical analyses of muscle tissues (Questions 1 and 3), physiologically-structured food web modeling informed by monitoring time-series (Questions 2 and 4), and a scoping workshop to address ecosystem services (Question 4). The investigators are using a "trans-basin" comparative approach to system-specific responses, studying fishes in Lake Erie, the Baltic Sea, and a Gulf of Mexico estuary. They study three species from each system that represent different degrees of benthic reliance, to discern differential responses to the increasingly hypoxic environment. This research provides novel insight about variable biotic responses to oxygen loss and the impacts on ecosystem functioning.</p> HypoxiDigm / Project Breathless largerWorkCitation project eng; USA biota oceans 13.27433 18.83864 55.18133 60.68278 2019-10-02 2021-10-20 Central Baltic Sea; Lake Erie; and Lavaca Bay, Texas 0 BCO-DMO catalogue of parameters from Stable isotope analyses, mercury measurements, and capture data from Baltic fishes collected 2019-2021 for Project Breathless Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/972053.rdf Name: Fish_ID Units: unitless Description: <p>Unique identifier for the sample</p> http://lod.bco-dmo.org/id/dataset-parameter/972054.rdf Name: Species Units: unitless Description: <p>Scientific name of the fish species</p> http://lod.bco-dmo.org/id/dataset-parameter/972055.rdf Name: Year Units: unitless Description: <p>Year of collection</p> http://lod.bco-dmo.org/id/dataset-parameter/972056.rdf Name: Date Units: unitless Description: <p>Date of collection</p> http://lod.bco-dmo.org/id/dataset-parameter/972057.rdf Name: Survey Units: unitless Description: <p>Name of the survey (BITS = Baltic International Trawl Survey; Gävle = a site near the city of Gävle; Herrvik = site off the eastern coast of Gotland; Muskö = site in the Stockholm Archipelago)</p> http://lod.bco-dmo.org/id/dataset-parameter/972058.rdf Name: Status Units: unitless Description: <p>Indicates oxygen conditions of the water at the sampling site (Hypoxic or Normoxic)</p> http://lod.bco-dmo.org/id/dataset-parameter/972059.rdf Name: Latitude Units: decimal degrees Description: <p>Latitude of fish capture</p> http://lod.bco-dmo.org/id/dataset-parameter/972060.rdf Name: Longitude Units: decimal degrees Description: <p>Longitude of fish capture</p> http://lod.bco-dmo.org/id/dataset-parameter/972061.rdf Name: Tot_Length Units: millimeters (mm) Description: <p>Fish total length</p> http://lod.bco-dmo.org/id/dataset-parameter/972062.rdf Name: Weight Units: grams (g) Description: <p>Fish fresh weight</p> http://lod.bco-dmo.org/id/dataset-parameter/972063.rdf Name: SLU_Age_years Units: years Description: <p>Ages in years estimated by trained otolith readers for cod collected by Baltic International Trawl Survey</p> http://lod.bco-dmo.org/id/dataset-parameter/972064.rdf Name: KL_Age_years Units: years Description: <p>Ages in years determined by PI Limburg </p> http://lod.bco-dmo.org/id/dataset-parameter/972065.rdf Name: Sex Units: unitless Description: <p>Sex of the fish</p> http://lod.bco-dmo.org/id/dataset-parameter/972066.rdf Name: d13C_VPDB Units: per mille Description: <p>Carbon stable isotopic ratios, using Vienna Pee Dee Belemnite standard</p> http://lod.bco-dmo.org/id/dataset-parameter/972067.rdf Name: d15N_Air Units: per mille Description: <p>Nitrogen stable isotopic ratios, using atmospheric N as the standard</p> http://lod.bco-dmo.org/id/dataset-parameter/972068.rdf Name: d34S_VCDT Units: per mille Description: <p>Sulfur stable isotopic ratios, using Vienna Canyon Diablo Triolite standard</p> http://lod.bco-dmo.org/id/dataset-parameter/972069.rdf Name: Tot_Hg_dry_weight Units: nanograms per gram (ng/g) Description: <p>Total mercury, ng/g dry weight</p> http://lod.bco-dmo.org/id/dataset-parameter/972070.rdf Name: Eye_Lens_Tot_Hg Units: nanograms per gram (ng/g) Description: <p>Eye lens total mercury (round goby only)</p> http://lod.bco-dmo.org/id/dataset-parameter/972071.rdf Name: Oto_Analyzed Units: unitless Description: <p>Indicates whether an otolith from the individual fish was analyzed (Y=yes, blank=no or not collected)</p> http://lod.bco-dmo.org/id/dataset-parameter/972072.rdf Name: Comments Units: unitless Description: <p>Comments</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact https://osprey.bco-dmo.org/dataset/967907/data/download download onLine dataset &lt;p&gt;Fish were collected during routine surveys performed for monitoring the status of fish stocks, either by national fishery management agencies (Department of Aquatic Resources, Swedish University of Agricultural Sciences, Icelandic Marine and Freshwater Research Institute) or international collaborative effort (Baltic International Trawl Survey). Fish were caught by trawl or by gillnet sets, then frozen for later processing.&lt;/p&gt; &lt;p&gt;In the lab, fish were measured for length and weighed. Sex and spawning stage were determined where possible. Otoliths and eye lenses were removed, cleaned, and stored until workup (see Related Datasets section below). Samples of dorsal muscle tissue were collected for mercury and stable isotope analysis and were dried to constant weight.&lt;/p&gt; &lt;p&gt;Total mercury analyses were conducted using atomic absorption spectrophotometry on a Milestone DMA-80 tri-cell unit (Milestone Srl, Italy). Briefly, the method involves thermal decomposition of a sample, amalgamation of mercury, and absorbance of mercury using a spectrophotometer.&amp;nbsp;Quality assurance and quality control was following EPA Method 7473, including daily calibration checks, the use of blanks, certified reference materials, and matrix spike/matrix spike duplicates. Sample duplicates were analyzed on a minimum of 20% of samples.&lt;/p&gt; &lt;p&gt;Stable isotopic ratio analyses for carbon (C), nitrogen (N), and sometimes sulfur (S)&amp;nbsp;were conducted by either the Stable Isotope Facility at the University of California at Davis or at the Cornell University Isotope Laboratory. Both facilities routinely analyze solid samples, such as ours, using elemental analyzers on the front end of isotope ratio mass spectrometers (EA-IRMS). Delta values are expressed relative to Vienna Pee Dee Belemnite (for carbon), air (for nitrogen), and Vienna Canyon Diablo Troilite (for sulfur) standards. Light stable isotopes of C and N were analyzed at UC Davis with&amp;nbsp;PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK). At Cornell, a Thermo Delta V isotope ratio mass spectrometer (IRMS) is interfaced to a NC2500 elemental analyzer (EA). Sulfur isotopes were analyzed separately on the same mass spectrometer at Cornell.&lt;/p&gt; &lt;p&gt;&lt;em&gt;(For otolith chemistry and fish eye lens chemistry results, see Related Datasets section below.)&lt;/em&gt;&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;Results data on total mercury and C, N, and S stable isotopes were received as-is from outside labs, with no further processing.&lt;/p&gt; Specified by the Principal Investigator(s) - loaded ... - ... - ... Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact NC2500 Elemental Analyzer NC2500 Elemental Analyzer PI Supplied Instrument Name: NC2500 Elemental Analyzer PI Supplied Instrument Description:At Cornell, an isotope ratio mass spectrometer was interfaced to a NC2500 elemental analyzer (EA).  Instrument Name: Carlo Erba NC 2500 elemental analyzer Instrument Short Name:Carlo Erba NC 2500 EA Instrument Description: A laboratory instrument that simultaneously determines total nitrogen, total hydrogen, and total carbon in a solid sample. The sample is completely and instantaneously oxidized by flash combustion, which converts all organic and inorganic substances into combustion products. The resulting combustion gases pass through a reduction furnace and are swept into the chromatographic column by the helium carrier gas. The gases are separated in the column and quantified or they can be introduced into another instrument, such as an isotope ratio mass spectrometer, for further analysis. The instrument was originally manufactured by CE instruments (formerly Carlo Erba) and has since been replaced by Thermo Scientific (part of Thermo Fisher Scientific). This model is no longer in production. Milestone DMA-80 tri-cell unit atomic absorption spectrophotometer Milestone DMA-80 tri-cell unit atomic absorption spectrophotometer PI Supplied Instrument Name: Milestone DMA-80 tri-cell unit atomic absorption spectrophotometer PI Supplied Instrument Description:Mercury analyses of tissues were made with a Milestone DMA-80 tri-cell unit atomic absorption spectrophotometer. Instrument Name: Milestone Direct Mercury Analyzer Instrument Short Name:DMA-80 Instrument Description: The Milestone DMA-80 is a mercury analyzer used to determine mercury concentrations in liquid and solid samples. The DMA-80 is based on the principles of sample thermal decomposition, mercury amalgamation, and atomic absorption detection. See more: https://milestonesci.com/direct-mercury-analyzer/ PDZ Europa 20-20 isotope ratio mass spectrometer PDZ Europa 20-20 isotope ratio mass spectrometer PI Supplied Instrument Name: PDZ Europa 20-20 isotope ratio mass spectrometer PI Supplied Instrument Description:Light stable isotopes of C and N were analyzed at UC Davis with elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK). Instrument Name: PDZ Europa 20-20 isotope ratio mass spectrometer Instrument Short Name:Europa 20-20 IRMS Instrument Description: The PDZ Europa 20-20 is a dedicated continuous flow isotope ratio mass spectrometer for hyphenated stable isotope analyses able to measure 15N, 13C, 18O, and 34S in a host of applications. The analyzer has been purposely designed to measure 2H by continuous flow methodology and is also suitable to analyze the light stable isotopes in all the commonly measured gases: H2, N2, NO, N2O, O2, CO, CO2, SO, and SO2.  PDZ Europa ANCA-GSL elemental analyzer  PDZ Europa ANCA-GSL elemental analyzer PI Supplied Instrument Name:  PDZ Europa ANCA-GSL elemental analyzer PI Supplied Instrument Description:Light stable isotopes of C and N were analyzed at UC Davis with PDZ Europa ANCA-GSL elemental analyzer interfaced to an isotope ratio mass spectrometer. Instrument Name: PDZ Europa ANCA-GSL elemental analyzer Instrument Short Name: Instrument Description: The ANCA-GSL module allows samples such as soil, viscous liquids, plant material, and organic compounds, to be analyzed directly by using Dumas combustion for 15N, 13C, and 34S or pyrolysis for 18O and D. It also allows isotope analysis of abundant gases from septum sealed containers. During combustion mode, a capsule containing the sample falls into the combustion tube and is converted in the presence of oxygen to CO2, N2, NOx, and H2O. An elemental copper stage reduces NOx, a MgClO4 trap removes water vapor, a switchable Carbosorb trap can be used to remove CO2 (for 15N only analyses) and a GC column separates CO2 from N2 (allowing dual isotope analysis). Modified packings, a Nafion dryer and different GC column allow 34S analysis. The sample preparation unit consists of a 66-place autosampler for unattended operation (larger options are available), 2 furnaces able to operate to 1100 deg C, and an on-board microprocessor. The analyzer is capable of dual isotope analysis of 15N and 13C. For CO (18O), H2 (2H), N2 (15N), CO2 (13C), CO2 (18O), and SO2 (34S) with precisions between 0.1 and 3 dependent on the element. Thermo Delta V isotope ratio mass spectrometer (IRMS) Thermo Delta V isotope ratio mass spectrometer (IRMS) PI Supplied Instrument Name: Thermo Delta V isotope ratio mass spectrometer (IRMS) PI Supplied Instrument Description:At Cornell, a Thermo Delta V isotope ratio mass spectrometer (IRMS) is interfaced to an elemental analyzer.  Instrument Name: Thermo Fisher Scientific DELTA V Advantage isotope ratio mass spectrometer Instrument Short Name:Delta V Advantage Instrument Description: The Thermo Scientific DELTA V Advantage is an isotope ratio mass spectrometer designed to measure isotopic, elemental, and molecular ratios of organic and inorganic compounds. The DELTA V Advantage is the standard model of the DELTA V series of isotope ratio mass spectrometers, which can be upgraded to the DELTA V Plus. The DELTA V Advantage can be operated in Continuous Flow or Dual Inlet mode. The standard collector configuration is the Universal Triple Collector. H2 collectors with online hydrogen capability are optional. The DELTA V Advantage is controlled by an automated, integrated Isodat software suite. A magnet, whose pole faces determine the free flight space for the ions, eliminates the traditional flight tube. The magnet is designed for fast mass switching which is further supported by a fast jump control between consecutive measurements of multiple gases within one run. The sample gas is introduced at ground potential, eliminating the need for insulation of the flow path, ensuring 100 percent transfer into the ion source. The amplifiers register ion beams up to 50 V. The DELTA V Advantage has a sensitivity of 1200 molecules per ion (M/I) in Dual Inlet mode and 1500 M/I in Continuous Flow mode. It has a system stability of < 10 ppm and an effective magnetic detection radius of 191 nm. It has a mass range of 1 - 80 Dalton at 3 kV.