http://lod.bco-dmo.org/id/dataset/968732
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-07-15
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
TEP and CSP particles collected from Station ALOHA for HOT time series during R/V Kilo Moana cruises from Jan 2020 to Sep 2022
2025-07-15
publication
2025-07-15
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-07-16
publication
https://doi.org/10.26008/1912/bco-dmo.968732.1
Robert T. Letscher
University of New Hampshire
principalInvestigator
Kieran Curran
University of New Hampshire
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Letscher, R. T., Curran, K. (2025) TEP and CSP particles collected from Station ALOHA for HOT time series during R/V Kilo Moana cruises from Jan 2020 to Sep 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-07-15 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.968732.1 [access date]
Dataset Description: <p>TEP = transparent exopolymer particle<br />
CSP = Coomassie stainable particle<br />
HOT = Hawaii Ocean Time Series<br />
Station ALOHA =&nbsp;Station ALOHA is the focal point of a range of oceanographic studies conducted over great temporal scale that intend to understand and explain the trends of the greater North Pacific Ocean<br />
NSF = National Science Foundation<br />
GF/F = glass fiber filter&nbsp;</p> Methods and Sampling: <p>TEP and CSP particles were collected onto 0.4 µm polycarbonate filters from 1 liter each of whole water collected via the CTD/Rosette Niskin bottles into 1 L polycarbonate media bottles. Filtration was achieved using a peristaltic pump and silicone tubing through a 25 mm polycarbonate filter holder. Filters containing particles were immediately stained with Alcian blue (TEP) or Coomassie blue (CSP) dyes and stored frozen at -20degC until analysis on shore back at the University of New Hampshire. TEP and CSP particle concentrations were measured following the spectrophotometric method of Bittar et al. 2018 in Limnology &amp; Oceanography Methods (doi:10.1002/lom3.10268) and standardized with xanthan gum and bovine albumen, respectively. Total dissolved carbohydrates were measured using the spectrophotometric method of Myklestad et al. 1997 in Marine Chemistry (doi:10.1016/S0304-4203(96)00074-6). Seawater samples were previously filtered via gravity filtration and silicone tubing connected to Niskin bottles at sea, passed through a 47mm glass fiber filter (GF/F; 0.7 µm) held in a polycarbonate filter holder, into 40 mL glass EPA vials with silicone septa tops and frozen at -20degC until analysis on shore back at the University of New Hampshire.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1923687 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1923687
completed
Robert T. Letscher
University of New Hampshire
603-862-1008
Earth Sciences & Ocean Process Analysis Laboratory 142 Morse Hall
Durham
NH
USA
robert.letscher@unh.edu
pointOfContact
Kieran Curran
University of New Hampshire
kieran.curran@unh.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cruise
Date
Longitude
Latitude
Depth
Transparent_Exopolymer_Particles
Coomassie_Stainable_Particles
Total_Dissolved_Carbohydrates
Masterflex L/S MasterSense Peristaltic Pump
Shimadzu UV-1800 Dual Beam UV-Vis Spectrophotometer
theme
None, User defined
Cruise identfier
date
longitude
latitude
depth
Transparent Exopolymer Particles
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Pump
UV Spectrophotometer-Shimadzu
instrument
BCO-DMO Standard Instruments
KM2108
HOT_cruises
service
Deployment Activity
subtropical North Pacific Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Transparent exopolymer and phytoplankton vertical migration as sources for preformed nitrate anomalies in the subtropical N. Pacific Ocean
https://osprey.bco-dmo.org/project/772658
Collaborative Research: Transparent exopolymer and phytoplankton vertical migration as sources for preformed nitrate anomalies in the subtropical N. Pacific Ocean
<p><em>NSF Award Abstract:</em><br />
The ocean is usually layered, with light and oxygen in the warmer surface and nutrients at the cooler depths. Biological and physical processes determine this distribution. Marine algae grow in the well-lit upper layers but need nutrients to grow. However, in the subtropics, the ocean's largest biome, the relationship between oxygen and nitrate (a key nutrient required for photosynthesis) is different from expected. Two processes could explain this. Nutrients could be transported upward by migrating giant single-celled algae (phytoplankton). Another explanation is that the production of an organic material called transparent exopolymer (TEP) takes up carbon without using nutrients or exporting carbon to depth, as would occur in photosynthesis. While both processes could be occurring, the relative contribution of migrating phytoplankton versus TEP would tell us whether the observed oxygen pattern in the upper ocean results from photosynthesis. This problem relates to the general question of where and how nutrients reach the well-lit surface waters to enable photosynthesis. These hypotheses are tested at the Hawaii Ocean Time-Series using in-situ camera systems to image and quantify the giant phytoplankton and direct water samples to measure the vertical distribution of TEP. The data are entered into numerical models to calculate the nitrate to oxygen relationships and add information about the carbon cycle. In addition to training of undergraduate students and a postdoctoral fellow, the cruises provide an opportunity to prepare a cadre of communication fellows who will develop materials and media, including videos, to translate this highly complex scientific concepts for the general public. The social media campaign #SaveOur70 provides a valuable venue to reach and engage with the public.</p>
<p>Quantifying nutrient transport, utilization, and its relationship to carbon drawdown in the subtropical gyres is fundamental to our understanding of the carbon cycle. Geochemical distributions from the well-characterized time-series sites near Hawaii and Bermuda have long-served to identify previously unknown links between subsurface nitrate fields, summertime dissolved inorganic carbon (DIC) drawdown, and net community production in the absence of known nutrient sources. Two recently suggested processes rise to prominence to explain anomalies in subtropical distributions of dissolved carbon, oxygen, and nitrate in the upper ocean: 1) nutrient transport by giant phytoplankton that vertically migrate, and 2) cycling of low N organic matter between the mixed layer and the upper nutricline as transparent exopolymer particles (TEP) or gel-like organic material (GLOM). While linked at a fundamental level (phytoplankton are TEP producers), the outcome of the two processes are distinct. Vertical migration of phytoplankton is an active transport of nitrate, acquired in the nutricline, to the surface. There is an implication of subsequent reduction, photosynthetic carbon fixation and eventual export. TEP/GLOM cycling results in apparent DIC drawdown but there is no net export out of the surface layer and no requirement for additional nutrient sources in the mixed layer. This project collects the data to quantify the contribution of these two processes to the observed anomalies in nitrate to oxygen distribution at the time-series station at Hawaii (HOT). This is accomplished by enumerating the vertically migrating, aflagellate flora (VMF), implementing a 1-D model on vertical migration, and coupling these results with a 1-D model of the contribution of N-poor carbon cycling patterns in the upper water column derived from TEP and carbohydrate measurements. The combined VMF and TEP/GLOM 1-D models are used to model the dissolved oxygen, carbon, and nitrate budgets at HOT allowing for attribution of both hypothesized processes to the observed preformed nitrate distribution, its formation rate, and summertime inorganic carbon drawdown.</p>
TEP and phytoplankton migration
largerWorkCitation
project
eng; USA
oceans
subtropical North Pacific Ocean
-158
-158
22.75
22.75
2020-01-31
2022-09-02
North Pacific Gyre, Hawaii Ocean Time-Series
0
BCO-DMO catalogue of parameters from TEP and CSP particles collected from Station ALOHA for HOT time series during R/V Kilo Moana cruises from Jan 2020 to Sep 2022
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/968751.rdf
Name: Cruise
Units: unitless
Description: <p>Cruise identifier. Integer cruise IDs correspond to HOT cruises (e.g. "319" is equivalent to "HOT-319")</p>
http://lod.bco-dmo.org/id/dataset-parameter/968752.rdf
Name: Date
Units: unitless
Description: <p>Date (ISO 8601 format)</p>
http://lod.bco-dmo.org/id/dataset-parameter/968753.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/968755.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/968757.rdf
Name: Depth
Units: meters (m)
Description: <p>Depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/968759.rdf
Name: Transparent_Exopolymer_Particles
Units: micrograms xanthan gum equivalents per liter (ug XG equiv./L)
Description: <p>Transparent Exopolymer Particles</p>
http://lod.bco-dmo.org/id/dataset-parameter/968760.rdf
Name: Coomassie_Stainable_Particles
Units: micrograms bovine albumen equivalents per liter (ug BA equiv./L)
Description: <p>Coomassie Stainable Particles</p>
http://lod.bco-dmo.org/id/dataset-parameter/968761.rdf
Name: Total_Dissolved_Carbohydrates
Units: micromolar (uM C)
Description: <p>Total Dissolved Carbohydrates</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
8104
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/b5376ab9-3468-45c1-8dbc-49dc4ca650c9/content
download
https://doi.org/10.26008/1912/bco-dmo.968732.1
download
onLine
dataset
<p>TEP and CSP particles were collected onto 0.4 µm polycarbonate filters from 1 liter each of whole water collected via the CTD/Rosette Niskin bottles into 1 L polycarbonate media bottles. Filtration was achieved using a peristaltic pump and silicone tubing through a 25 mm polycarbonate filter holder. Filters containing particles were immediately stained with Alcian blue (TEP) or Coomassie blue (CSP) dyes and stored frozen at -20degC until analysis on shore back at the University of New Hampshire. TEP and CSP particle concentrations were measured following the spectrophotometric method of Bittar et al. 2018 in Limnology &amp; Oceanography Methods (doi:10.1002/lom3.10268) and standardized with xanthan gum and bovine albumen, respectively. Total dissolved carbohydrates were measured using the spectrophotometric method of Myklestad et al. 1997 in Marine Chemistry (doi:10.1016/S0304-4203(96)00074-6). Seawater samples were previously filtered via gravity filtration and silicone tubing connected to Niskin bottles at sea, passed through a 47mm glass fiber filter (GF/F; 0.7 µm) held in a polycarbonate filter holder, into 40 mL glass EPA vials with silicone septa tops and frozen at -20degC until analysis on shore back at the University of New Hampshire.</p>
Specified by the Principal Investigator(s)
* Table within submitted file "HOT_TEP_CSP_2020_2022.csv" was imported into the BCO-DMO data system for this dataset. Values "-999" imported as missing data values. Table will appear as Data File: 968732_v1_tep-and-csp-hot.csv (along with other download format options).
Missing Data Identifiers:
* In the BCO-DMO data system, missing data identifiers are displayed according to the format of data you access. For example, in csv files it will be blank (null) values. In Matlab .mat files it will be NaN values. When viewing data online at BCO-DMO, the missing value will be shown as blank (null) values.
* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores. Can not start with a number]
* Date converted to ISO 8601 format
* Prefix "HOT-" add to numeric cruise ids for consistency with HOT program. Cruise ID km2108 case changed to KM2108 for consistency with other datasets and cruise databases.
* trailing zeros added for lat lon to clarify precision for all values should be hundredths place. The data submitter explained the integers in the lat, lon columns not rounded to degree (e.g. -158 is precision 158.00).
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Masterflex L/S MasterSense Peristaltic Pump
Masterflex L/S MasterSense Peristaltic Pump
PI Supplied Instrument Name: Masterflex L/S MasterSense Peristaltic Pump Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps
Shimadzu UV-1800 Dual Beam UV-Vis Spectrophotometer
Shimadzu UV-1800 Dual Beam UV-Vis Spectrophotometer
PI Supplied Instrument Name: Shimadzu UV-1800 Dual Beam UV-Vis Spectrophotometer Instrument Name: UV Spectrophotometer-Shimadzu Instrument Short Name:UV Spectrophotometer-Shimadzu Instrument Description: The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Cruise: KM2108
KM2108
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM2108
Robert T. Letscher
Cruise: HOT_cruises
HOT_cruises
Multiple Vessels
vessel
HOT_cruises
Angelicque E. White
University of Hawaiʻi at Mānoa
http://hahana.soest.hawaii.edu/hot/
Report describing HOT_cruises
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
Multiple Vessels
vessel