Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • Data Processing Description
  • BCO-DMO Processing Description
  • Problem Description
• Data Files
• Related Publications
• Parameters
• Instruments
• Deployments
• Project Information
• Funding

Samples were collected in April 2017 aboard the R/V Oceanus using Niskin bottles. Nutrient samples were analyzed for NH₄⁺ and NO₂^- aboard the ship, with additional shore-based analyses of combined NO₃^-+NO₂^- and PO₄^3- at the University of California Santa Barbara Marine Science Institute Analytical Lab (see Beman et al. 2020). Water column profiles of oxygen consumption rates (OCR) were measured using 5 replicates at each depth, including one each with tracer level (5-10% in situ concentration measured at sea) addition of ¹⁵NH₄⁺ or ¹⁵NO₂^- to measure ammonia/nitrite oxidation. OCR was measured based on starting and ending DO values, and samples for nitrite and ammonia oxidation were collected from labeled bottles at the end of the experiments. Nitrite and ammonia oxidation rates followed Beman et al. (2012) and Beman et al. (2013). See Beman et al. (preprint) for additional detail.

Data were processed and analyzed in Microsoft Excel and R.

- Imported original file "BCODMO_BEMAN_ETNP17.xlsx" into the BCO-DMO system.
- Flagged "nd" as a missing data value (missing data are empty/blank in the final CSV file).
- Converted Date column to YYYY-MM-DD format.
- Converted Time column to hh:mm format and renamed to "Time_Local".
- Renamed fields to comply with BCO-DMO naming conventions.
- Saved final file as "969971_v1_etnp17_nutrients_and_rates.csv".

NSF Award Abstract:
Aerobic microorganisms in the ocean help regulate biogeochemical nutrient cycles through the linked production and consumption of dissolved oxygen (DO) and organic matter. Concentrations of DO have been shown to be steadily decreasing in deeper ocean waters and the resulting areas of critically low DO content, known as oxygen minimum zones (OMZs), are expanding. While this phenomenon is recognized as having potentially serious impacts on biogeochemical cycles where OMZs are growing, surprisingly little research has been done to identify the mechanisms and quantify the specific processes that will affect these changes. This project will study the connection between changing DO concentrations and nutrient cycling in the interior of the ocean. The oxidation of nitrogen compounds (ammonia and nitrite in particular) is strongly influenced by DO concentrations. Because of the complex and connected nature of marine biogeochemical reactions that involve DO, each one potentially altered by concentration changes, this research is critical for a complete understanding of how ocean chemistry will change in the near future. The project will incorporate education into the research by developing materials to teach high school students about the nitrogen cycle, by developing a marine chemistry based course for undergraduates that will give them both field and computational experience, and by building on past efforts to include traditionally underrepresented groups in science. One of the graduate students funded by this project will translate lectures into Spanish and make these available on the internet for increased accessibility for minority students.

The world's largest oxygen minimum zone (OMZ), located in the Eastern Tropical North Pacific (ETNP), is an ideal study site for research into the effects of varying dissolved oxygen (DO) concentrations on nutrient cycling in the interior ocean. Throughout this OMZ, the extent of anoxia exhibits a range that allows for study of the effect of changing DO content on the rates and mechanisms that control consumption of DO and organic matter by aerobic microorganisms in a 'real world' setting. In particular, ammonia and nitrite oxidation, reactions that play a critical role in the nitrogen cycle, are likely to be significantly affected by varying DO concentration. This project will evaluate aerobic respiration, ammonia, and nitrite oxidation rates at various stations in the ETNP, examine carefully controlled incubations, and develop primers to target active microorganisms in the OMZ; all to quantify the connections between DO and these biogeochemical nutrient cycles. The research will also examine the hypothesis that more organic carbon is respired in waters with low DO and a shallow OMZ than previously thought and evaluate the possibility that nitrogen cycling in low DO regions could push OMZs to anoxia due to nitrite oxidation coupled with nitrate reduction, which could potentially accelerate DO consumption. With the expansion of OMZ's in a changing ocean, it is crucial to more fully understand the connections between these various, complex, components.