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            <gco:CharacterString>Cite this dataset as: Chavarry, J., Choy, C. A. (2025) Bulk tissue stable isotope analysis of zooplankton samples collected by MOCNESS from R/V Sally Ride and R/V Roger Revelle cruises in the southern California Current Ecosystem from 2020-2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-08-22 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.971975.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Bulk SIA of gelatinous zooplankton Dataset Description:  Methods and Sampling: &amp;lt;p&amp;gt;Gelatinous zooplankton were collected on four research cruises between 2020 and 2023 at seven stations representing four nearshore and escarpment, and two offshore regions within the Southern California Bight. We conducted depth‑discrete sampling of gelatinous zooplankton using a 10-square-meter (m²) Multiple Opening/Closing Net and Environmental Sensing System (MOCNESS) equipped with five depth-discrete nets (mesh sizes 5-millimeter (mm), Wiebe et al., 1985). The 10 m² MOCNESS was towed obliquely as the ship traveled at a speed between 1 to 2 knots, with depth-discrete collections occurring on the upcast. Sampling stations and depth intervals varied across stations and cruises, but there were typically two depth intervals sampled within the upper 500 meters (m) and larger depth intervals below 500 m. The maximum depth of sampling increased from 1,250 m nearshore to 3,000 m offshore, corresponding with the deepening of the water column.&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Upon recovery, samples were stored in chilled seawater and kept at 5 degrees Celsius (°C) until processing. All sample processing was performed on ice to preserve body condition. Gelatinous zooplankton were identified to the most specific taxonomic level using published keys. The concentration of carbon and/or nitrogen can be low in gelatinous individuals (Lüskow et al., 2021), so we pooled multiple gelatinous individuals into a single sample, while standardizing size ranges. Individuals within a taxonomic group were split into relative size classes. The minimum, median, and maximum lengths of individuals within each size class were recorded to the nearest millimeter. Bell diameter was measured for medusae, body diameter for ctenophores, and total length for pelagic tunicates, molluscs, and chaetognaths. The number of individuals per size class was counted and then weighed as a group to the nearest 0.01 gram (g) using a motion‑compensating scale (Marel M2400), which was routinely calibrated at sea following manufacturer instructions. Samples were stored in Whirl‑Paks at ‑80°C until further processing in the laboratory.&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
561 samples of gelatinous zooplankton representing 13 taxonomic groups were chosen for bulk tissue stable carbon and nitrogen isotope analysis. Gelatinous zooplankton were briefly thawed to remove visible gut contents using forceps and a scalpel, which were cleaned with ethanol between samples. Both gelatinous zooplankton and mesozooplankton samples were then lyophilized and homogenized in Whirl‑Paks. To ensure sufficient sample mass for stable isotope analysis, samples often contained multiple individuals from the same net, taxonomic group, and size class. The number of individuals per sample was typically fewer than 100, with a larger number of individuals pooled for some samples of &amp;lt;em&amp;gt;Pantachogon&amp;lt;/em&amp;gt; spp. and &amp;lt;em&amp;gt;Hormiphora&amp;lt;/em&amp;gt; spp.&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Dried, homogenized tissues were packaged into tin capsules (1.5 to 4 milligrams (mg) per sample) for bulk tissue stable isotope analyses, which were conducted at the University of Hawaii at Manoa and the University of California Merced. Briefly, samples were run on a Costech 4010 Elemental Combustion System coupled to either a ThermoScientific DELTA V Advantage, ThermoScientific DELTA V+, or a ThermoFinnigan DeltaPlus XP isotope ratio mass spectrometer through a ThermoScientific Conflo IV interface. Stable isotope values are reported in the standard per mille notation (‰), compared to the standards atmospheric N₂and Vienna Pee Dee Belemnite for nitrogen and carbon, respectively. To ensure accuracy and instrument precision, both labs used a combination of international reference materials (from the United States Geological Survey or the National Institute of Standards and Technology) and in‑house reference materials (squid or tuna) with known δ15N and δ13C values. Based on analyzed reference materials, sample reproducibility was ± 0.2‰ for samples run at the University of Hawaii at Manoa, and sample reproducibility was ± 0.4‰ for samples run at UC Merced.&amp;lt;/p&amp;gt;</gco:CharacterString>
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This CAREER award is advancing our understanding of connections between surface and deep water ocean food webs, which in turn has important implications for carbon cycling in the ocean. Although marine ecosystems deeper than 200 m encompass Earth’s largest single habitat, the food web relationships of deep-sea organisms are poorly resolved. The investigator is evaluating active transport by fishes, squids, crustaceans, and gelatinous animals that move organic matter from the more productive surface into deeper waters through feeding and diel vertical migration. She is using a combination of data on abundance and distribution of species with measurements of stable isotope biomarkers to understand trophic relationships and connect community composition and migratory behavior with food-web processes in the southern California Current ecosystem. The investigator is from a group traditionally underrepresented in science, and she has designed a comprehensive educational plan to train a more diverse, inclusive generation of seagoing biological oceanographers through hands-on field and research experiences. In addition to providing support for graduate and undergraduate students to participate directly in this research, the investigator is creating a novel and cohesive undergraduate curriculum involving a seagoing laboratory course to teach interdisciplinary field methods to conduct research on pelagic ecosystems and a seminar course highlighting Native and Indigenous knowledge alongside more traditional oceanographic research. The overall goal is to broaden participation in science by combining hands-on interdisciplinary research, mentoring, and expanding networks of minority and majority scientists.&lt;/p&gt;
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	Description: &lt;p&gt;sample identifier&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983165.rdf
	Name: phylum
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http://lod.bco-dmo.org/id/dataset-parameter/983166.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/983167.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/983168.rdf
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	Units: unitless
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http://lod.bco-dmo.org/id/dataset-parameter/983169.rdf
	Name: net
	Units: unitless
	Description: &lt;p&gt;the net number when the sample was collected&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983170.rdf
	Name: Date_UTC
	Units: unitless
	Description: &lt;p&gt;date the sample was collected (UTC)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983171.rdf
	Name: year
	Units: unitless
	Description: &lt;p&gt;the year the sample was collected (UTC)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983172.rdf
	Name: month
	Units: unitless
	Description: &lt;p&gt;the month the sample was collected (UTC)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983173.rdf
	Name: day
	Units: unitless
	Description: &lt;p&gt;the day the sample was collected (UTC)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983174.rdf
	Name: specimen_count
	Units: number of individuals
	Description: &lt;p&gt;the number of individual gelatinous zooplankton in that sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983175.rdf
	Name: EA_mass
	Units: milligrams
	Description: &lt;p&gt;the mass of the dried sample analyzed on the elemental  analyzer&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983176.rdf
	Name: nitrogen_ug
	Units: micrograms
	Description: &lt;p&gt;the sample&amp;#039;s nitrogen mass detected  by the elemental analyzer&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983177.rdf
	Name: d15N_bulk
	Units: parts per thousand
	Description: &lt;p&gt;nitrogen isotope ratio relative to atmospheric N2 detected by the isotope ratio mass spectrometer&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983178.rdf
	Name: carbon_ug
	Units: micrograms
	Description: &lt;p&gt;the sample&amp;#039;s carbon mass detected  by the elemental analyzer&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983179.rdf
	Name: d13C_bulk
	Units: parts per thousand
	Description: &lt;p&gt;carbon isotope ratio relative to Vienna Pee Dee Belemnite detected by the isotope ratio mass spectrometer&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983180.rdf
	Name: sia_lab
	Units: unitless
	Description: &lt;p&gt;University of Hawaii at Manoa&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983181.rdf
	Name: SIA_reproducibility
	Units: parts per thousand
	Description: &lt;p&gt;the reproducibility of the stable isotope ratio values, as reported by the stable isotope labs&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983182.rdf
	Name: length_type
	Units: unitless
	Description: &lt;p&gt;the way the gelatinous zooplankton individuals were measured dependent on taxonomy, either bell diameter, body diameter, or total length&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983183.rdf
	Name: median_length_mm
	Units: millimeters
	Description: &lt;p&gt;the median length of individuals in a sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983184.rdf
	Name: taxa_wmd_rounded_25_m
	Units: meters
	Description: &lt;p&gt;the weighted mean depth of the taxonomic grouping across all locations and sampling years, rounded to the nearest 25 m depth&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983185.rdf
	Name: min_depth_m
	Units: meters
	Description: &lt;p&gt;the minimum depth of collection for the net that collected the sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983186.rdf
	Name: max_depth_m
	Units: meters
	Description: &lt;p&gt;the maximum depth of collection for the net that collected the sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983187.rdf
	Name: mean_depth_m
	Units: meters
	Description: &lt;p&gt;the mean depth of collection for the net that collected the sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983188.rdf
	Name: latitude
	Units: decimal degrees
	Description: &lt;p&gt;the latitude of where the sample was collected&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983189.rdf
	Name: longitude
	Units: decimal degrees
	Description: &lt;p&gt;the longitude of where the sample was collected&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983190.rdf
	Name: sampling_location
	Units: unitless
	Description: &lt;p&gt;the region within the southern California Current Ecosystem where the sample was collected, either &amp;quot;nearshore&amp;quot;, &amp;quot;escarpment&amp;quot;, or &amp;quot;offshore&amp;quot;&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983191.rdf
	Name: sampling_time
	Units: unitless
	Description: &lt;p&gt;the time of sample collection, either &amp;quot;day&amp;quot; or &amp;quot;night&amp;quot;&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983192.rdf
	Name: season
	Units: unitless
	Description: &lt;p&gt;the season of collection, either &amp;quot;summer&amp;quot; or &amp;quot;fall&amp;quot;&lt;/p&gt; 
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                <gco:CharacterString>&amp;lt;p&amp;gt;Gelatinous zooplankton were collected on four research cruises between 2020 and 2023 at seven stations representing four nearshore and escarpment, and two offshore regions within the Southern California Bight. We conducted depth‑discrete sampling of gelatinous zooplankton using a 10-square-meter (m²) Multiple Opening/Closing Net and Environmental Sensing System (MOCNESS) equipped with five depth-discrete nets (mesh sizes 5-millimeter (mm), Wiebe et al., 1985). The 10 m² MOCNESS was towed obliquely as the ship traveled at a speed between 1 to 2 knots, with depth-discrete collections occurring on the upcast. Sampling stations and depth intervals varied across stations and cruises, but there were typically two depth intervals sampled within the upper 500 meters (m) and larger depth intervals below 500 m. The maximum depth of sampling increased from 1,250 m nearshore to 3,000 m offshore, corresponding with the deepening of the water column.&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Upon recovery, samples were stored in chilled seawater and kept at 5 degrees Celsius (°C) until processing. All sample processing was performed on ice to preserve body condition. Gelatinous zooplankton were identified to the most specific taxonomic level using published keys. The concentration of carbon and/or nitrogen can be low in gelatinous individuals (Lüskow et al., 2021), so we pooled multiple gelatinous individuals into a single sample, while standardizing size ranges. Individuals within a taxonomic group were split into relative size classes. The minimum, median, and maximum lengths of individuals within each size class were recorded to the nearest millimeter. Bell diameter was measured for medusae, body diameter for ctenophores, and total length for pelagic tunicates, molluscs, and chaetognaths. The number of individuals per size class was counted and then weighed as a group to the nearest 0.01 gram (g) using a motion‑compensating scale (Marel M2400), which was routinely calibrated at sea following manufacturer instructions. Samples were stored in Whirl‑Paks at ‑80°C until further processing in the laboratory.&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
561 samples of gelatinous zooplankton representing 13 taxonomic groups were chosen for bulk tissue stable carbon and nitrogen isotope analysis. Gelatinous zooplankton were briefly thawed to remove visible gut contents using forceps and a scalpel, which were cleaned with ethanol between samples. Both gelatinous zooplankton and mesozooplankton samples were then lyophilized and homogenized in Whirl‑Paks. To ensure sufficient sample mass for stable isotope analysis, samples often contained multiple individuals from the same net, taxonomic group, and size class. The number of individuals per sample was typically fewer than 100, with a larger number of individuals pooled for some samples of &amp;lt;em&amp;gt;Pantachogon&amp;lt;/em&amp;gt; spp. and &amp;lt;em&amp;gt;Hormiphora&amp;lt;/em&amp;gt; spp.&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Dried, homogenized tissues were packaged into tin capsules (1.5 to 4 milligrams (mg) per sample) for bulk tissue stable isotope analyses, which were conducted at the University of Hawaii at Manoa and the University of California Merced. Briefly, samples were run on a Costech 4010 Elemental Combustion System coupled to either a ThermoScientific DELTA V Advantage, ThermoScientific DELTA V+, or a ThermoFinnigan DeltaPlus XP isotope ratio mass spectrometer through a ThermoScientific Conflo IV interface. Stable isotope values are reported in the standard per mille notation (‰), compared to the standards atmospheric N₂and Vienna Pee Dee Belemnite for nitrogen and carbon, respectively. To ensure accuracy and instrument precision, both labs used a combination of international reference materials (from the United States Geological Survey or the National Institute of Standards and Technology) and in‑house reference materials (squid or tuna) with known δ15N and δ13C values. Based on analyzed reference materials, sample reproducibility was ± 0.2‰ for samples run at the University of Hawaii at Manoa, and sample reproducibility was ± 0.4‰ for samples run at UC Merced.&amp;lt;/p&amp;gt;</gco:CharacterString>
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