http://lod.bco-dmo.org/id/dataset/982174
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-08-10
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Excitation Emission Matrices (EEM) during long-term incubation experiments of Synechococcus DOM conducted from Nov 2020 to Nov 2021
2025-08-10
publication
2025-08-10
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-11-04
publication
https://doi.org/10.26008/1912/bco-dmo.982174.1
Michael Gonsior
University of Maryland Center for Environmental Science
principalInvestigator
Feng Chen
University of Maryland Center for Environmental Science
principalInvestigator
Leanne Powers
State University of New York College of Environmental Science and Forestry
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Gonsior, M., Chen, F., Powers, L. (2025) Excitation Emission Matrices (EEM) during long-term incubation experiments of Synechococcus DOM conducted from Nov 2020 to Nov 2021. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-08-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.982174.1 [access date]
Methods and Sampling: <p>Field seawater samples for all incubation experiments were collected from the Gulf Stream off the Coast of Wilmington, North Carolina, in November of&nbsp;2020.&nbsp;<br />
<br />
Samples were filtered through Whatman GF/F filters prior to analyses. A Horiba Aqualog Fluoromeeter was used to acquire the EEM spectra. All spectra were inner filter corrected, scatter corrected and normalized to the Water Raman peak area.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1829888 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1829888
completed
Michael Gonsior
University of Maryland Center for Environmental Science
410-326-7245
146 Williams Street
Solomons
MD
20688-3204
USA
gonsior.michael@gmail.com
pointOfContact
Feng Chen
University of Maryland Center for Environmental Science
410-234-8866
chenf@umces.edu
pointOfContact
Leanne Powers
State University of New York College of Environmental Science and Forestry
315-470-6854
lcpowers@esf.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Horiba Aqualog
theme
None, User defined
Spectrometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The fate of lysis products of picocyanobacteria contributes to marine humic-like chromophoric dissolved organic matter
https://osprey.bco-dmo.org/project/806071
The fate of lysis products of picocyanobacteria contributes to marine humic-like chromophoric dissolved organic matter
<p><em>NSF Award Abstract:</em><br />
This study focuses on the sources and composition of colored dissolved organic matter (CDOM) in the ocean. CDOM is a part of water that absorbs sunlight. This material is important because it filters out harmful ultraviolet radiation. Scientists use it to track the movement of carbon and other important biological and chemical processes in the ocean. Organisms such as algae living in the open ocean have been shown to be sources of CDOM, but the chemical composition of these algal natural products remains to be discovered. Recent results from studying common algae show that viruses may break down algal cells and release material that looks like CDOM. This study will use new tools to find out if viruses and algae are creating this material and study its chemical makeup. This project will support two graduate students and provide summer internships for undergraduates through the NSF Research Experiences for Undergraduates (REU) program. The investigators will participate in a range of education and outreach activities.</p>
<p>The sources and structural nature of marine CDOM within the oceans remain unclear and continue to be a subject of debate. Marine in situ sources of CDOM have been suggested and some have been confirmed, but thus far none could explain the ubiquitous appearance of the so called "humic-like" CDOM component. Unique features of this component include its unusual exponential behavior in ultraviolet-visible (UV-Vis) absorbance with the absorbance extending well above 400 nm, and the large Stoke's shift in fluorescence spectroscopy. Picocyanobacteria are ubiquitous in the World's Oceans and make up 50 % of the autotrophic marine primary production. Preliminary results showed that the picocyanobacteria Synechococcus and Prochlorococcus release CDOM that matched the "humic-like" appearance of globally observed marine CDOM after virus-induced lysis. The main focus of this study is the characterization of the optical properties and molecular composition of viral-lysed DOM (VDOM) from different strains of Synechococcus and Prochlorococcus and additionally Trichodesmium which was shown in a previous study to also release CDOM. Associations between the chemical characterization information and metagenomics and transcriptomics data will be investigated for picocyanobacteria in the Pacific and Atlantic Oceans. This study includes long-term incubation experiments to determine the persistence of picocyanobacteria-derived CDOM as well as changes in microbial communities and processes (gene expression) that are related to the degradation of VDOM during the incubation period.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Picocyanobacteria CDOM
largerWorkCitation
project
eng; USA
oceans
2020-11-01
2021-11-30
Bermuda Atlantic Time Series Station (BATS) and station Aloha, Hawaii
0
BCO-DMO catalogue of parameters from Excitation Emission Matrices (EEM) during long-term incubation experiments of Synechococcus DOM conducted from Nov 2020 to Nov 2021
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2599307
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/3fc133c5-2504-420f-bbec-7a022dadd366/content
download
1557037
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/e1f5997b-f115-46a3-8357-6dcab6a8e08c/content
download
https://doi.org/10.26008/1912/bco-dmo.982174.1
download
onLine
dataset
<p>Field seawater samples for all incubation experiments were collected from the Gulf Stream off the Coast of Wilmington, North Carolina, in November of&nbsp;2020.&nbsp;<br />
<br />
Samples were filtered through Whatman GF/F filters prior to analyses. A Horiba Aqualog Fluoromeeter was used to acquire the EEM spectra. All spectra were inner filter corrected, scatter corrected and normalized to the Water Raman peak area.</p>
Specified by the Principal Investigator(s)
<p>EEM data were collected using the Horiba Aqualog Fluorometer, and all Fluorescence spectra were blank corrected, inner filter corrected and normalized to the Water Raman scattering.&nbsp;&nbsp;&nbsp;<br />
<br />
Software: Horiba Aqualog software version 4.3<br />
<br />
Data are presented in two separate zip packages:<br />
982174_v1_<strong>CONTROL</strong>.zip = All EEM data of the triplicate controls at each time point (no Synechococcus DOM added).<br />
982174_v1_<strong>VDOM</strong>.zip&nbsp;= <em>All EEM data of the triplicate Synechococcus DOM added samples at each time point during the one-year incubation.</em></p>
<p>All files are the direct exported files from the Horiba Aqualog software and can be opened directly in MS Excel or other software. The first part of the file name is indicative of the sample collection time in days. For example, T0 means beginning of incubation, T01 means day 1 of the experiment and so on. &nbsp;The s annotation is presenting the number of replicate. For example the control sample T0s1 is replicate one at the beginning of experiment, T0s2 is the second replicate, and T0S3 is the third replicate. &nbsp;For the VDOM samples, the replicate numbering is T0S4 for the first replicate.T0S5 for the second, and T0S6 for the third replicate, as an example. The remaining attributes of the file naming indicates the processing of the file prior to export. &nbsp;RM means scatter correction or Raleigh masking (RM), IFE corresponds to inner filter correction (IFE), which uses the simultaneously measured absorbance data, and NRM means it was normalized to the Water Raman Scattering.&nbsp;</p>
Specified by the Principal Investigator(s)
Version 1:
* Unix command "dos2unix" was used on the .dat files to fix some encoding issues in some of the files.
* .dat files zipped into two packages and attached to page 982174_v1_CONTROL.zip and 982174_v1_VDOM.zip
* File inventory with md5sum added as a supplemental file.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Horiba Aqualog
Horiba Aqualog
PI Supplied Instrument Name: Horiba Aqualog Instrument Name: Spectrometer Instrument Short Name:Spectrometer Instrument Description: A spectrometer is an optical instrument used to measure properties of light over a specific portion of the electromagnetic spectrum. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0460/