http://lod.bco-dmo.org/id/dataset/982177
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-08-10
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Biochemical data on dissolved organic matter composition from Galveston Bay collected on five one-day trips aboard R/V Trident following Hurricane Harvey from September 4 to 28, 2017
2025-08-20
publication
2025-08-20
revision
BCO-DMO Linked Data URI
2025-08-20
creation
http://lod.bco-dmo.org/id/dataset/982177
Karl Kaiser
Texas A&M, Galveston
principalInvestigator
Cédric Fichot
Boston University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Kaiser, K., Fichot, C. (2025) Biochemical data on dissolved organic matter composition from Galveston Bay collected on five one-day trips aboard R/V Trident following Hurricane Harvey from September 4 to 28, 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-08-20 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/982177 [access date]
Coastal DOM Galveston Bay Dataset Description: Methods and Sampling: <p>Surface water samples were collected on five consecutive one-day trips aboard R/V Trident along a transect from the Port of Houston to the Galveston Bay entrance following Hurricane Harvey from September 4 to September 28, 2017. Samples were filtered on board through 0.2-micrometer (μm) Whatman-Nucleopore Q-TEC filters (Filtration Solutions) for dissolved organic carbon (DOC), optical, and chemical analysis.</p>
<p>Concentrations of DOC were measured by high-temperature catalytic oxidation using a Shimadzu TOC-V total organic carbon analyzer. Deep seawater reference standards (Consensus Reference Program, University of Miami) were used to assure the accuracy of DOC measurements. Absorbance was measured in a 1-centimeter (cm) quartz cuvette from 200 to 800 nanometers (nm) using a dual-beam spectrophotometer (UV-1800, Shimadzu) with Milli-Q water as the reference blank. Specific UV absorbance (SUVA254 ) was determined by dividing the UV absorbance at 254 nm by the DOC concentration. The spectral slope (S275−295) was calculated using the linear regression of natural log-transformed absorption spectra (Helms et al., 2008).</p>
<p>Samples for dissolved lignin (900 milliliters (mL)) were acidified to pH 2.5 using 6 moles per liter (mol L-1) sulfuric acid and extracted through Agilent PPL cartridges (1 gram (g)) at 10 milliliters per minute (mL min-1). After extraction, cartridges were rinsed with 10 mL of deionized water acidified to pH 2.5 and dried for 30 seconds to remove residual water. The cartridges were eluted with 20 mL of methanol at 2 mL min-1, and the eluate was stored in glass vials at -20 degrees Celsius until analysis. Concentrations of lignin phenols were determined using ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry after CuSO4 oxidation, following the methods described in Yan and Kaiser (2018a,b). Aliquots of methanol extracts (∼30 microgram (μg) sample OC content) were dried in reaction vials and re-suspended in 200 microliters (μL) of 1.1 mol L-1 argon-sparged NaOH, followed by addition of 10 μL of 10 millimoles per liter (mmol L-1) CuSO4 and 10 μL of 0.2 mol L-1 ascorbic acid. Reaction vials were vigorously mixed and placed into 60-mL pressure-tight Teflon vessels filled with 5 mL of 1 mol L-1 NaOH. The oxidation was conducted at 150 degrees Celsius for 120 minutes. Sample solutions were spiked with 13C-labeled surrogate standards and purified with Waters HLB cartridges (30 milligram (mg), 1 mL). Separation and detection of lignin phenols was performed on an Agilent Infinity 1260 series UHPLC system coupled to an Agilent 6420 QqQ detector operating in alternating positive and negative modes with dynamic multiple reaction monitoring. Eleven lignin phenols were determined in all samples, including vanillyl phenols (V; vanillin, acetovanillone, and vanillic acid), syringyl phenols (S; syringaldehyde, acetosyringone, and syringic acid), p-hydroxyl phenols (P; p-hydroxybenzaldehyde, p-hydroxyacetophenone, and p-hydroxybenzoic acid), and cinnamyl phenols (C; p-coumaric acid and ferulic acid).</p>
<p>Total hydrolyzable enantiomeric dissolved amino acids (free and combined), including L-and D- forms of aspartic acid, glutamic acid, serine, histidine, threonine, glycine, arginine, alanine, tyrosine, valine, isoleucine, phenylalanine, leucine, and lysine were analyzed using high-performance liquid chromatography and fluorescence detection. After microwave-assisted vapor phase hydrolysis (Kaiser and Benner, 2005), amino acid monomers were derivatized with a mixture of N-isobutyryl- L-cysteine and o-phthaldialdehyde and separated on an Agilent Poroshell 120 EC-C18 column (4.6 millimeters (mm) × 100 mm, 2.7 μm). A binary solvent system was employed: mobile phase A was 48 mmol L-1 KH2PO4 with pH adjusted to 6.25, and mobile phase B was methanol/acetonitrile (13/1, v/v). The linear gradient program was: 0% B at 0 minutes, 39% B at 13.3 minutes, 54% B at 19.2 minutes, 60% B at 21.3 minutes, 80% B at 22 minutes, and hold at 80% B for 1 minute. The flow rate was 1.5 mL min-1 and column temperature was maintained at 35 degrees Celsius. Excitation and emission wavelength of the detector was set to 330 nm and 450 nm, respectively. Racemization of amino acid enantiomers occurring during acidic hydrolysis was corrected using the average rates determined on free and protein amino acids (Kaiser and Benner, 2005). Total D-amino acids (D-AA) was defined as the sum of the four D-enantiomers of aspartic acid (D-Asx), glutamic acid (D-Glx), serine (D-Ser), and alanine (D-Ala), which were ubiquitously present in all samples.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2148831 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2148831
onGoing
Karl Kaiser
Texas A&M, Galveston
409-740-4879
1001 Texas Clipper Rd Dept. of Marine Sciences, Texas A&M University, Galveston Campus
Galveston
TX
77554
USA
kaiserk@tamug.edu
pointOfContact
Cédric Fichot
Boston University
617-358-2182
685 Commonwealth Ave.
Boston
MA
02215
USA
cgfichot@bu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
Station
Lat
Long
Salinity
DOC
a254
SUVA
S275_295
S350_400
SR
HIX
BIX
FI
C1
C2
C3
C4
PAL
PON
PAD
VAL
VON
VAD
SAL
SON
SAD
CAD
FAD
TDLP9
P_V
S_V
C_V
Ad_AlV
Ad_AlS
His
Thr
Gly
Arg
Tyr
Val
Ileu
Phe
Leu
Lys
Ala
Ser
Glx
Asx
DAA
THAA
Agilent 6420 QqQ detector
Shimadzu TOC-V total organic carbon analyzer
Agilent Infinity 1260 series UHPLC system
dual-beam spectrophotometer (UV-1800, Shimadzu)
theme
None, User defined
date
site
latitude
longitude
salinity
dissolved organic Carbon
absorbance
No BCO-DMO term
particulate organic nitrogen
amino acid concentration
featureType
BCO-DMO Standard Parameters
Quadrupole Mass Spectrometer
Shimadzu TOC-V Analyzer
Ultra-high-performance liquid chromatography
UV Spectrophotometer-Shimadzu
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Distribution and Cycling of Carboxyl-Rich Alicyclic Molecules (CRAM) in the Ocean
https://osprey.bco-dmo.org/project/894132
Collaborative Research: Distribution and Cycling of Carboxyl-Rich Alicyclic Molecules (CRAM) in the Ocean
<p><strong>NSF Award Abstract:</strong><br />
Collaborative Research: Distribution and Cycling of Carboxyl-Rich Alicyclic Molecules (CRAM) in the Ocean</p>
<p>Dissolved organic matter is the largest pool of organic carbon in the ocean. Thus, it plays an important role in carbon storage and climate change. The components of dissolved organic matter are the remains of biological products. As a result, the chemical make-up of dissolved organic matter (DOM) can provide important clues about the processes affecting this carbon pool. By learning more about the chemical structures and reactions that produce DOM, connections can be made to biological and biochemical processes and their importance. This project will study the distribution, production, and removal of one important class of molecules called carboxyl-rich alicyclic molecules (CRAM). These molecules are abundant in marine DOM and play an important role in the ocean carbon cycle. They also affect the supply of the micronutrient iron, and thus have an important influence on primary production and ocean food webs. The research includes field and laboratory experiments and takes advantage of ocean locations with long-term data. Together, the lead scientists will provide training and resources for the graduate students, undergraduate research opportunities for underrepresented groups in STEM, and direct outreach to the public through a website, articles in local newspapers, and talks at public venues.</p>
<p>CRAM make-up 8 to 25% of marine dissolved organic carbon (DOC) and represent a carbon reservoir of >65 Pg C that is equivalent to at least 8% of the atmospheric CO2 pool. CRAM play a central role in the functioning of the microbial carbon pump, a conceptual framework for producing refractory carbon by microbial processes in the ocean. Further, CRAM act as an important ligand of Fe(III) in the ocean, thereby playing an important role in regulating marine primary production. The main project objectives are: (1) measure the variable distribution of CRAM in the ocean, and (2) determine the dominant mechanisms of CRAM production and removal. The project will leverage an extensive set of already collected samples across the world’s oceans and new field work within the framework of the Bermuda Atlantic and Hawaii Ocean Time Series. Field measurements will be combined with experimental approaches to specifically address microbial and photochemical reactions leading to CRAM production and removal. This research will fill important gaps in resolving the key questions about organic carbon cycling in the ocean and explore the role of DOM in mediating the cycles of iron and other trace elements.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
CRAM in the ocean
largerWorkCitation
project
eng; USA
oceans
-94.9789
-94.6886
29.33278
29.6725
2017-09-04
2017-09-28
0
BCO-DMO catalogue of parameters from Biochemical data on dissolved organic matter composition from Galveston Bay collected on five one-day trips aboard R/V Trident following Hurricane Harvey from September 4 to 28, 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/982817.rdf
Name: Date
Units: unitless
Description: <p>Date sample was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/982818.rdf
Name: Station
Units: unitless
Description: <p>Site identification</p>
http://lod.bco-dmo.org/id/dataset-parameter/982819.rdf
Name: Lat
Units: degrees
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/982820.rdf
Name: Long
Units: degrees
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/982821.rdf
Name: Salinity
Units: psu
Description: <p>Salinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/982822.rdf
Name: DOC
Units: micromoles per liter (umol L-1)
Description: <p>Dissolved organic carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/982823.rdf
Name: a254
Units: per meter (m-1)
Description: <p>absorption coefficient at 254 nm</p>
http://lod.bco-dmo.org/id/dataset-parameter/982824.rdf
Name: SUVA
Units: L mgC-1 m-1
Description: <p>CDOM absorbance parameter</p>
http://lod.bco-dmo.org/id/dataset-parameter/982825.rdf
Name: S275_295
Units: unitless
Description: <p>Spectral slope range 275-295 nm</p>
http://lod.bco-dmo.org/id/dataset-parameter/982826.rdf
Name: S350_400
Units: unitless
Description: <p>Spectral slope range 350-450 nm</p>
http://lod.bco-dmo.org/id/dataset-parameter/982827.rdf
Name: SR
Units: unitless
Description: <p>Spectral slope ratio 275-295/350-400</p>
http://lod.bco-dmo.org/id/dataset-parameter/982828.rdf
Name: HIX
Units: unitless
Description: <p>Humification Index (HIX); DOM composition metric</p>
http://lod.bco-dmo.org/id/dataset-parameter/982829.rdf
Name: BIX
Units: unitless
Description: <p>BIX; DOM composition metric</p>
http://lod.bco-dmo.org/id/dataset-parameter/982830.rdf
Name: FI
Units: unitless
Description: <p>Fluorescence Index (FI); DOM composition metric</p>
http://lod.bco-dmo.org/id/dataset-parameter/982831.rdf
Name: C1
Units: Raman units
Description: <p>Fluorescence intensity of component 1</p>
http://lod.bco-dmo.org/id/dataset-parameter/982832.rdf
Name: C2
Units: Raman units
Description: <p>Fluorescence intensity of component 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/982833.rdf
Name: C3
Units: Raman units
Description: <p>Fluorescence intensity of component 3</p>
http://lod.bco-dmo.org/id/dataset-parameter/982834.rdf
Name: C4
Units: Raman units
Description: <p>Fluorescence intensity of component 4</p>
http://lod.bco-dmo.org/id/dataset-parameter/982835.rdf
Name: PAL
Units: nanomoles per liter (nmol L-1)
Description: <p>p-hydroxy-benzaldehyde</p>
http://lod.bco-dmo.org/id/dataset-parameter/982836.rdf
Name: PON
Units: nanomoles per liter (nmol L-1)
Description: <p>p-hydroxy-acetophenone</p>
http://lod.bco-dmo.org/id/dataset-parameter/982837.rdf
Name: PAD
Units: nanomoles per liter (nmol L-1)
Description: <p>p-hydroxy-benzoic acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/982838.rdf
Name: VAL
Units: nanomoles per liter (nmol L-1)
Description: <p>vanillin</p>
http://lod.bco-dmo.org/id/dataset-parameter/982839.rdf
Name: VON
Units: nanomoles per liter (nmol L-1)
Description: <p>acetovanillone</p>
http://lod.bco-dmo.org/id/dataset-parameter/982840.rdf
Name: VAD
Units: nanomoles per liter (nmol L-1)
Description: <p>vanillin acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/982841.rdf
Name: SAL
Units: nanomoles per liter (nmol L-1)
Description: <p>syringealdehyde</p>
http://lod.bco-dmo.org/id/dataset-parameter/982842.rdf
Name: SON
Units: nanomoles per liter (nmol L-1)
Description: <p>acetosyringone</p>
http://lod.bco-dmo.org/id/dataset-parameter/982843.rdf
Name: SAD
Units: nanomoles per liter (nmol L-1)
Description: <p>syringic acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/982844.rdf
Name: CAD
Units: nanomoles per liter (nmol L-1)
Description: <p>coumaric acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/982845.rdf
Name: FAD
Units: nanomoles per liter (nmol L-1)
Description: <p>ferulic acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/982846.rdf
Name: TDLP9
Units: nanomoles per liter (nmol L-1)
Description: <p>sum of 9 total dissolved lignin phenols</p>
http://lod.bco-dmo.org/id/dataset-parameter/982847.rdf
Name: P_V
Units: [not provided]
Description: <p>[not provided]</p>
http://lod.bco-dmo.org/id/dataset-parameter/982848.rdf
Name: S_V
Units: [not provided]
Description: <p>[not provided]</p>
http://lod.bco-dmo.org/id/dataset-parameter/982849.rdf
Name: C_V
Units: [not provided]
Description: <p>[not provided]</p>
http://lod.bco-dmo.org/id/dataset-parameter/982850.rdf
Name: Ad_AlV
Units: [not provided]
Description: <p>[not provided]</p>
http://lod.bco-dmo.org/id/dataset-parameter/982851.rdf
Name: Ad_AlS
Units: [not provided]
Description: <p>[not provided]</p>
http://lod.bco-dmo.org/id/dataset-parameter/982852.rdf
Name: His
Units: nanomoles per liter (nmol L-1)
Description: <p>histidine</p>
http://lod.bco-dmo.org/id/dataset-parameter/982853.rdf
Name: Thr
Units: nanomoles per liter (nmol L-1)
Description: <p>threonine</p>
http://lod.bco-dmo.org/id/dataset-parameter/982854.rdf
Name: Gly
Units: nanomoles per liter (nmol L-1)
Description: <p>glycine</p>
http://lod.bco-dmo.org/id/dataset-parameter/982855.rdf
Name: Arg
Units: nanomoles per liter (nmol L-1)
Description: <p>arginine</p>
http://lod.bco-dmo.org/id/dataset-parameter/982856.rdf
Name: Tyr
Units: nanomoles per liter (nmol L-1)
Description: <p>tyrosine</p>
http://lod.bco-dmo.org/id/dataset-parameter/982857.rdf
Name: Val
Units: nanomoles per liter (nmol L-1)
Description: <p>valine</p>
http://lod.bco-dmo.org/id/dataset-parameter/982858.rdf
Name: Ileu
Units: nanomoles per liter (nmol L-1)
Description: <p>isoleucine</p>
http://lod.bco-dmo.org/id/dataset-parameter/982859.rdf
Name: Phe
Units: nanomoles per liter (nmol L-1)
Description: <p>phenylalanine</p>
http://lod.bco-dmo.org/id/dataset-parameter/982860.rdf
Name: Leu
Units: nanomoles per liter (nmol L-1)
Description: <p>leucine</p>
http://lod.bco-dmo.org/id/dataset-parameter/982861.rdf
Name: Lys
Units: nanomoles per liter (nmol L-1)
Description: <p>lysine</p>
http://lod.bco-dmo.org/id/dataset-parameter/982862.rdf
Name: Ala
Units: nanomoles per liter (nmol L-1)
Description: <p>alanine</p>
http://lod.bco-dmo.org/id/dataset-parameter/982863.rdf
Name: Ser
Units: nanomoles per liter (nmol L-1)
Description: <p>serine</p>
http://lod.bco-dmo.org/id/dataset-parameter/982864.rdf
Name: Glx
Units: nanomoles per liter (nmol L-1)
Description: <p>glutamic acid and glutamine</p>
http://lod.bco-dmo.org/id/dataset-parameter/982865.rdf
Name: Asx
Units: nanomoles per liter (nmol L-1)
Description: <p>aspartic acid and asparagine</p>
http://lod.bco-dmo.org/id/dataset-parameter/982866.rdf
Name: DAA
Units: nanomoles per liter (nmol L-1)
Description: <p>sum of dissolved D-amino acids</p>
http://lod.bco-dmo.org/id/dataset-parameter/982867.rdf
Name: THAA
Units: nanomoles per liter (nmol L-1)
Description: <p>total hydrolyzable amino acids</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/982177/data/download
download
onLine
dataset
<p>Surface water samples were collected on five consecutive one-day trips aboard R/V Trident along a transect from the Port of Houston to the Galveston Bay entrance following Hurricane Harvey from September 4 to September 28, 2017. Samples were filtered on board through 0.2-micrometer (μm) Whatman-Nucleopore Q-TEC filters (Filtration Solutions) for dissolved organic carbon (DOC), optical, and chemical analysis.</p>
<p>Concentrations of DOC were measured by high-temperature catalytic oxidation using a Shimadzu TOC-V total organic carbon analyzer. Deep seawater reference standards (Consensus Reference Program, University of Miami) were used to assure the accuracy of DOC measurements. Absorbance was measured in a 1-centimeter (cm) quartz cuvette from 200 to 800 nanometers (nm) using a dual-beam spectrophotometer (UV-1800, Shimadzu) with Milli-Q water as the reference blank. Specific UV absorbance (SUVA254 ) was determined by dividing the UV absorbance at 254 nm by the DOC concentration. The spectral slope (S275−295) was calculated using the linear regression of natural log-transformed absorption spectra (Helms et al., 2008).</p>
<p>Samples for dissolved lignin (900 milliliters (mL)) were acidified to pH 2.5 using 6 moles per liter (mol L-1) sulfuric acid and extracted through Agilent PPL cartridges (1 gram (g)) at 10 milliliters per minute (mL min-1). After extraction, cartridges were rinsed with 10 mL of deionized water acidified to pH 2.5 and dried for 30 seconds to remove residual water. The cartridges were eluted with 20 mL of methanol at 2 mL min-1, and the eluate was stored in glass vials at -20 degrees Celsius until analysis. Concentrations of lignin phenols were determined using ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry after CuSO4 oxidation, following the methods described in Yan and Kaiser (2018a,b). Aliquots of methanol extracts (∼30 microgram (μg) sample OC content) were dried in reaction vials and re-suspended in 200 microliters (μL) of 1.1 mol L-1 argon-sparged NaOH, followed by addition of 10 μL of 10 millimoles per liter (mmol L-1) CuSO4 and 10 μL of 0.2 mol L-1 ascorbic acid. Reaction vials were vigorously mixed and placed into 60-mL pressure-tight Teflon vessels filled with 5 mL of 1 mol L-1 NaOH. The oxidation was conducted at 150 degrees Celsius for 120 minutes. Sample solutions were spiked with 13C-labeled surrogate standards and purified with Waters HLB cartridges (30 milligram (mg), 1 mL). Separation and detection of lignin phenols was performed on an Agilent Infinity 1260 series UHPLC system coupled to an Agilent 6420 QqQ detector operating in alternating positive and negative modes with dynamic multiple reaction monitoring. Eleven lignin phenols were determined in all samples, including vanillyl phenols (V; vanillin, acetovanillone, and vanillic acid), syringyl phenols (S; syringaldehyde, acetosyringone, and syringic acid), p-hydroxyl phenols (P; p-hydroxybenzaldehyde, p-hydroxyacetophenone, and p-hydroxybenzoic acid), and cinnamyl phenols (C; p-coumaric acid and ferulic acid).</p>
<p>Total hydrolyzable enantiomeric dissolved amino acids (free and combined), including L-and D- forms of aspartic acid, glutamic acid, serine, histidine, threonine, glycine, arginine, alanine, tyrosine, valine, isoleucine, phenylalanine, leucine, and lysine were analyzed using high-performance liquid chromatography and fluorescence detection. After microwave-assisted vapor phase hydrolysis (Kaiser and Benner, 2005), amino acid monomers were derivatized with a mixture of N-isobutyryl- L-cysteine and o-phthaldialdehyde and separated on an Agilent Poroshell 120 EC-C18 column (4.6 millimeters (mm) × 100 mm, 2.7 μm). A binary solvent system was employed: mobile phase A was 48 mmol L-1 KH2PO4 with pH adjusted to 6.25, and mobile phase B was methanol/acetonitrile (13/1, v/v). The linear gradient program was: 0% B at 0 minutes, 39% B at 13.3 minutes, 54% B at 19.2 minutes, 60% B at 21.3 minutes, 80% B at 22 minutes, and hold at 80% B for 1 minute. The flow rate was 1.5 mL min-1 and column temperature was maintained at 35 degrees Celsius. Excitation and emission wavelength of the detector was set to 330 nm and 450 nm, respectively. Racemization of amino acid enantiomers occurring during acidic hydrolysis was corrected using the average rates determined on free and protein amino acids (Kaiser and Benner, 2005). Total D-amino acids (D-AA) was defined as the sum of the four D-enantiomers of aspartic acid (D-Asx), glutamic acid (D-Glx), serine (D-Ser), and alanine (D-Ala), which were ubiquitously present in all samples.</p>
Specified by the Principal Investigator(s)
<p>Instrument data were organized in Excel spreadsheets. Mass spectrometry data were processed with Matlab code provided in Fu et al. (2020). Final data were prepared with Anaconda Python distribution 2023.09-0, Python version 3.11.5.</p>
Specified by the Principal Investigator(s)
- Imported original file "Chemical_data_GB.csv" into the BCO-DMO system.
- Marked "NA" as a missing data value (missing data are empty/blank in the final CSV).
- Converted Date column to YYYY-MM-DD format.
- Renamed fields to comply with BCO-DMO naming conventions.
- Saved the final file as "982177_v1_coastal_dom_galveston_bay.csv".
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Agilent 6420 QqQ detector
Agilent 6420 QqQ detector
PI Supplied Instrument Name: Agilent 6420 QqQ detector Instrument Name: Quadrupole Mass Spectrometer Instrument Short Name:QMS Instrument Description: A piece of apparatus that consists of an ion source, a mass-to-charge analyser, a detector and a vacuum system and is used to measure mass spectra. The detector is a quadrupole mass-to-charge analyser, which holds the ions in a stable orbit by an electric field generated by four parallel electrodes.
Shimadzu TOC-V total organic carbon analyzer
Shimadzu TOC-V total organic carbon analyzer
PI Supplied Instrument Name: Shimadzu TOC-V total organic carbon analyzer Instrument Name: Shimadzu TOC-V Analyzer Instrument Short Name:Shimadzu TOC-V Instrument Description: A Shimadzu TOC-V Analyzer measures DOC by high temperature combustion method. Community Standard Description: http://onto.nerc.ac.uk/CAST/124
Agilent Infinity 1260 series UHPLC system
Agilent Infinity 1260 series UHPLC system
PI Supplied Instrument Name: Agilent Infinity 1260 series UHPLC system Instrument Name: Ultra-high-performance liquid chromatography Instrument Short Name:UHPLC Instrument Description: Ultra high-performance liquid chromatography: Column chromatography where the mobile phase is a liquid, the stationary phase consists of very small (< 2 microm) particles and the inlet pressure is relatively high.
dual-beam spectrophotometer (UV-1800, Shimadzu)
dual-beam spectrophotometer (UV-1800, Shimadzu)
PI Supplied Instrument Name: dual-beam spectrophotometer (UV-1800, Shimadzu) Instrument Name: UV Spectrophotometer-Shimadzu Instrument Short Name:UV Spectrophotometer-Shimadzu Instrument Description: The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/