Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • Data Processing Description
  • BCO-DMO Processing Description
  • Problem Description
• Related Datasets
• Parameters
• Instruments
• Project Information
• Funding

This dataset is part of Project Breathless, which brings together an interdisciplinary team of ecologists, economists and communication scholars to increase understanding of the impact of low oxygen dead zones in lakes and oceans.  The project team focuses on how low oxygen conditions influence fish, their habitats and the food webs that support them, as well as ecosystem services, including fisheries production.

This dataset is one of three examining Lake Erie fishes using data collected during routine surveys for monitoring the status of fish stocks:

• Dataset 982348 (Lake Erie fishes capture data) summarizes capture data as well as basic biological information; it also includes all stable isotopic ratio and total mercury data collected from muscle tissue and eye lenses. 
• Dataset 982372 (this dataset of Lake Erie fishes otolith chemistry) contains all of the Lake Erie fish (round goby and yellow perch) otolith chemistry data.
• Dataset 982386 (Lake Erie fishes eye lens chemistry) contains all of the eye lens chemistry data for round goby and yellow perch.

(See Related Datasets section below for links)

Fish sampling, basic biological data, and tissues collection 

Fish samples were provided from routine monitoring surveys by the Ohio Department of Natural Resources (Fairport Harbor, OH) and by the USGS Lake Erie Biological Station (Sandusky, OH). Dates and locations of trawling are provided. Fish were caught by trawl or by gillnet sets. Fish were frozen for later processing.

In the lab, fish were measured (length, weight); sex was determined if possible. Otoliths and eye lenses were removed, cleaned, and stored dry until workup. Samples of dorsal muscle tissue were collected (away from the skin) for mercury and stable isotope analysis and were dried to constant weight, then freeze-dried.

Otolith preparation and analysis (Lake Erie_2 dataset).

Otoliths were embedded in clean epoxy (Struers EpoFix), then sectioned to expose the core and polished. Trace elemental concentrations were quantified via laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) at SUNY ESF. The surfaces of transversely sectioned otoliths were ablated by a 193 nm Teledyne CETAC Analyte Excite Excimer Laser Ablation System which was coupled to a Thermo iCAP TQ ICPMS instrument. by running a laser ablation transect across the polished sections from one edge to the other; the ablated material was swept via a helium carrier gas into an argon plasma, which ionized the material that was subsequently counted by a mass spectrometer in single-quadrupole mode. Samples were sectioned and polished to expose the interior cores; analyses were made by running the laser across the surface from one edge, through the core, and out the other side.

Otolith transects were typically made with an 85-micron circular spot size, driven at a rate of 4 microns/second, fluence set to 1.8 J/cm², and a laser repetition rate of 10 Hz. Materials-appropriate standards (for otoliths MACS3 carbonate and MAPS4 phosphate standards from the USGS) were run at the start and end of the run, and then periodically during each analysis day to calibrate and correct for instrument drift. 

The analytes collected were 12 trace elements (B, Mg, P, Ca, Mn, Fe, Cu, Zn, Br, Sr, I, and Ba). Results in this dataset are in units of parts per million (ppm). Note that some outliers are included, but most outliers exceeding 2 standard deviations were removed and values were interpolated from adjacent replicates. In addition, calibrations seem elevated, judging from the calcium values which should be closer to 380,000-40,000 ppm. However, if a user expresses other trace elements in ratio to calcium, these are robust values.

ICP-MS data files containing counts per second of each trace element were produced; each transect analysis had its own data file, but they have been concatenated here for archiving.

(For fish eye lens chemistry, total mercury analyses, and stable isotopic ratio analyses, see the Related Datasets section below.)

Otolith and eye lens trace elemental data were processed in Excel files. Calibrations made over the course of the day were fit to plots of the calibrants as a function of time to produce drift corrections. Processing of the raw data included removal of spurious spikes (> 2 SD) and subsequent interpolation. Raw data were then calibrated and drift-corrected simultaneously, to produce data as parts per million.

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NSF Award Abstract:
Ocean oxygen loss (deoxygenation) is increasing due to climate warming. This warming, together with nutrient loading, is causing many marine and freshwater systems to experience increasing episodes of hypoxia (low oxygen) of greater duration and intensity. Impacts on fish and fisheries have been difficult to quantify; direct observation has been challenged by a lack of long-term exposure indicators. This team has successfully refined the use of fish chemical biomarkers in fish otoliths (earstones) to directly assess lifetime hypoxia exposure in fishes. This project will those findings to look for additional biomarkers and models, to expand our understanding of how hypoxia affects fish and their food webs, contaminant transfers, and ecosystem services including economic impacts. The project includes a unique way of training students in science communication, posing the question: What forms of media and "messaging strategies" about deoxygenation are most effective at raising public awareness and understanding? Students are developing entries for PlanetForward's Storyfest, which is a contest to tell compelling stories to foster environmental understanding and solutions. Students from historically underrepresented, economically disadvantaged backgrounds are particularly sought out to participate. The investigators will engage with regional, national, and international management agencies and other relevant stakeholder groups to share information.

This project encompasses a novel, linked set of interdisciplinary studies of food webs, and ecosystem services assessment. The thematic questions explored in this project are: 1. How does hypoxia alter habitat use for fishes? 2. How does hypoxia-altered habitat use and habitat productivity change food webs? 3. How does hypoxia affect/enhance trophic transfer of methylmercury? 4. How do hypoxia-induced changes in food webs affect aquatic ecosystem services? The set of linked studies will employ chemical analyses of otoliths and eye lenses, combined with chemical analyses of muscle tissues (Questions 1 and 3), physiologically-structured food web modeling informed by monitoring time-series (Questions 2 and 4), and a scoping workshop to address ecosystem services (Question 4). The investigators are using a "trans-basin" comparative approach to system-specific responses, studying fishes in Lake Erie, the Baltic Sea, and a Gulf of Mexico estuary. They study three species from each system that represent different degrees of benthic reliance, to discern differential responses to the increasingly hypoxic environment. This research provides novel insight about variable biotic responses to oxygen loss and the impacts on ecosystem functioning.