http://lod.bco-dmo.org/id/dataset/984065 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2025-09-09 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Nutrient data for samples collected every 4 hours from Orcas Island, WA, USA Coastal Ocean (2m depth) during the period from 2021-05-27 to 2021-06-18 2025-09-09 publication 2025-09-09 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2025-09-19 publication https://doi.org/10.26008/1912/bco-dmo.984065.1 Julia Kubanek Georgia Institute of Technology principalInvestigator Brook L. Nunn University of Washington principalInvestigator Tatiana A. Rynearson University of Rhode Island principalInvestigator Miranda Mudge University of Washington principalInvestigator Emma Timmins-Schiffman University of Washington principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Nunn, B. L., Kubanek, J., Rynearson, T. A., Timmins-Schiffman, E., Mudge, M., Bartlett, E. (2025) Nutrient data for samples collected every 4 hours from Orcas Island, WA, USA Coastal Ocean (2m depth) during the period from 2021-05-27 to 2021-06-18. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-09-09 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.984065.1 [access date] Dataset Description: &lt;p&gt;Additional funding description:&lt;br /&gt; This dataset was supported by NSF OCE-2401646, OCE-2401645, OCE-2401644, University of Washington Royalty Research Fund, NIH NIEHS grant R21ES034337-01, NSF IOS-2041497, NIH fellowship F31 ES032733-01A1&lt;/p&gt; Methods and Sampling: &lt;p&gt;Water samples for nutrients were collected by rinsing a 1 L Pyrex media bottle and a 60 mL HDPE syringe in triplicate with whole water. From a fresh collection of 1 L, we filtered 50 mL whole water using the cleaned syringe through a 25 mm 0.45 µm cellulose syringe filter (Nalgene, surfactant-free). Filtered water samples were collected in triple-rinsed 50 mL HDPE bottles and stored immediately at −20 °C. Bottles were transferred to the University of Washington for long-term storage at −20 °C.&lt;/p&gt; &lt;p&gt;Methodology is from the results paper&amp;nbsp;Nunn et al. (2024, doi:10.1038/s41597-024-04013-5).&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2401646 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2401646 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2401645 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2401645 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2401644 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2401644 Funding provided by National Institute of Environmental Health Sciences (NIEHS) Award Number: R21ES034337-01 Award URL: https://reporter.nih.gov/search/wmUb13OUa0C-1UrPp0t1kQ/project-details/10497336 Funding provided by NSF Division of Integrative Organismal Systems (NSF IOS) Award Number: IOS-2041497 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2041497 Funding provided by National Institutes of Health (NIH) Award Number: ES032733-01A1 Award URL: https://reporter.nih.gov/search/b7x9vIROQ0-SYmLKyS0NFw/project-details/10312280 completed Julia Kubanek Georgia Institute of Technology 404-894-8424 School of Biological Sciences Georgia Institute of Technology Atlanta GA 30332-0230 USA julia.kubanek@biosci.gatech.edu pointOfContact Brook L. Nunn University of Washington 206-616-9023 Department of Genome Sciences, Foege Building S113 3720 15th Ave NE Seattle WA 98195 USA brookh@uw.edu pointOfContact Tatiana A. Rynearson University of Rhode Island 401-874-6022 Graduate School of Oceanography, University of Rhode Island 215 South Ferry Rd. Narragansett RI 02882 USA rynearson@uri.edu pointOfContact Miranda Mudge University of Washington mudge832@gmail.com pointOfContact Emma Timmins-Schiffman University of Washington emmats@uw.edu pointOfContact asNeeded Dataset Version: 1 Unknown Date_PT Time_PT DateID_PT ISO_DateTime_UTC Phosphate_Concentration Silicate_Concentration Nitrate_Concentration Nitrite_Concentration Ammonium_Concentration Latitude Longitude HDPE bottles theme None, User defined date_local time_local Date and time ISO_DateTime_UTC reactive phosphorus (PO4) No BCO-DMO term Nitrate Nitrite Ammonium latitude longitude featureType BCO-DMO Standard Parameters High density polyethylene water bottle instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Rhythm and Blooms: Deciphering metabolic, functional and taxonomic interactions over the life cycle of a phytoplankton bloom https://osprey.bco-dmo.org/project/931507 Collaborative Research: Rhythm and Blooms: Deciphering metabolic, functional and taxonomic interactions over the life cycle of a phytoplankton bloom <p><em>NSF Award Abstract:</em><br /> Floating, single-celled algae, or phytoplankton, form the base of marine food webs. When phytoplankton have sufficient nutrients to grow quickly and generate dense populations, known as blooms, they influence productivity of the entire food web, including rich coastal fisheries. The present research explores how the environment (nutrients) as well as physical and chemical interactions between individual cells in a phytoplankton community and their associated bacteria act to control the timing of bloom events in a dynamic coastal ecosystem. The work reveals key biomolecules within the base of the food web that can inform food web functioning (including fisheries) and be used in global computational models that forecast the impacts of phytoplankton activities on global carbon cycling. A unique set of samples and data collected in 2021 and 2022 that captured phytoplankton and bacterial communities before, during, and after phytoplankton blooms, is analyzed using genomic methods and the results are used to interrogate these communities for biomolecules associated with blooms stages. The team mentors undergraduates, graduate students, and postdoctoral researchers in the fields of biochemical oceanography, genome sciences, and time-series multivariate statistics. University of Washington organized hackathons develop publicly accessible portals for the simplified interrogation and visualization of 'omics data by high schoolers and undergraduates and are implemented in investigator-led undergraduate teaching modules and the University of Rhode Island Ocean Classroom. The research team also returns to Orcas Island, WA, where the field sampling takes place, to host a series of annual Science Weekends to foster scientific engagement with the local community.</p> <p>Phytoplankton blooms, from initiation to decline, play vital roles in biogeochemical cycling by fueling primary production, influencing nutrient availability, impacting carbon sequestration in aquatic ecosystems, and supporting secondary production. In addition to environmental conditions, the physical and chemical interactions between individual phytoplankton can significantly modulate blooms, influencing the growth, maintenance, and senescence of phytoplankton. Recent work in steady-state open ocean ecosystems has shown that important chemicals are transferred amongst plankton on time-dependent metabolic schedules that are related to diel cycles. It is unknown how these metabolic schedules operate in dynamic coastal environments that experience perturbations, such as phytoplankton blooms. Here, the investigators are examining metabolic scheduling using long-term, diel sample sets to reveal how chemical and biological signals associated with the initiation, maintenance, and cessation of phytoplankton blooms are modulated on both short (hrs) and long (days-weeks) time scales. Findings are advancing the ability to predict and manage phytoplankton dynamics, providing crucial insights into ecological stability and future oceanographic sampling strategies. Additionally, outcomes of this study are providing a new foundational understanding of the succession of microbial communities and their chemical interactions across a range of timescales. In the long term, this research has the potential to identify predictors of the timing of phytoplankton blooms, optimize fisheries management, and guide future research on carbon sequestration.</p> Rhythm and Blooms largerWorkCitation project eng; USA oceans -122.883233 -122.883233 48.6765 48.6765 2021-05-27 2021-06-18 Coastal waters East Sound WA 0 BCO-DMO catalogue of parameters from Nutrient data for samples collected every 4 hours from Orcas Island, WA, USA Coastal Ocean (2m depth) during the period from 2021-05-27 to 2021-06-18 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/984084.rdf Name: Date_PT Units: unitless Description: <p>date the sample was collected. Local time zone US/Pacific (PST/PDT).</p> http://lod.bco-dmo.org/id/dataset-parameter/984085.rdf Name: Time_PT Units: unitless Description: <p>time the sample was collected. Local time zone US Pacific (PST/PDT).</p> http://lod.bco-dmo.org/id/dataset-parameter/984360.rdf Name: DateID_PT Units: unitless Description: <p>character value for the combined date and time of sample collection. Local time zone US/Pacific (PST/PDT).</p> http://lod.bco-dmo.org/id/dataset-parameter/984086.rdf Name: ISO_DateTime_UTC Units: unitless Description: <p>Contains DateTime with timezone of sample collection in ISO 8601 format (UTC time zone).</p> http://lod.bco-dmo.org/id/dataset-parameter/984087.rdf Name: Phosphate_Concentration Units: millimolar (mM) Description: <p>Phosphate concentrations</p> http://lod.bco-dmo.org/id/dataset-parameter/984088.rdf Name: Silicate_Concentration Units: millimolar (mM) Description: <p>Silicate concentrations</p> http://lod.bco-dmo.org/id/dataset-parameter/984089.rdf Name: Nitrate_Concentration Units: millimolar (mM) Description: <p>Nitrate concentrations</p> http://lod.bco-dmo.org/id/dataset-parameter/984090.rdf Name: Nitrite_Concentration Units: millimolar (mM) Description: <p>Nitrite concentrations</p> http://lod.bco-dmo.org/id/dataset-parameter/984091.rdf Name: Ammonium_Concentration Units: millimolar (mM) Description: <p>Ammonium concentrations</p> http://lod.bco-dmo.org/id/dataset-parameter/984092.rdf Name: Latitude Units: decimal degrees Description: <p>Latitudinal coordinate of where sample was collected</p> http://lod.bco-dmo.org/id/dataset-parameter/984093.rdf Name: Longitude Units: decimal degrees Description: <p>Longitudinal coordinate of where sample was collected</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 12899 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/15060671-2859-41cb-812e-84fb9722d7ba/content download https://doi.org/10.26008/1912/bco-dmo.984065.1 download onLine dataset &lt;p&gt;Water samples for nutrients were collected by rinsing a 1 L Pyrex media bottle and a 60 mL HDPE syringe in triplicate with whole water. From a fresh collection of 1 L, we filtered 50 mL whole water using the cleaned syringe through a 25 mm 0.45 µm cellulose syringe filter (Nalgene, surfactant-free). Filtered water samples were collected in triple-rinsed 50 mL HDPE bottles and stored immediately at −20 °C. Bottles were transferred to the University of Washington for long-term storage at −20 °C.&lt;/p&gt; &lt;p&gt;Methodology is from the results paper&amp;nbsp;Nunn et al. (2024, doi:10.1038/s41597-024-04013-5).&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;Total nutrient analysis was performed in triplicate by the University of Washington Marine Chemistry Lab to determine concentrations of nitrate, nitrite, ammonium, silicate, and phosphate following standard methods outlined in UNESCO, 1994 (doi: &lt;span style=&quot;font-size:13px&quot;&gt;10.25607/obp-1409)&lt;/span&gt;.&lt;/p&gt; &lt;p&gt;Data processing is from the results paper Nunn et al. (2024, doi:10.1038/s41597-024-04013-5).&lt;/p&gt; Specified by the Principal Investigator(s) * Sheet 1 of submitted file &quot;Nunn_OrcasIsland_Data_Nutrients.xlsx&quot; was exported as csv and imported into the BCO-DMO data system for this dataset.  Table will appear as Data File: 984065_v1_nutrients.csv (along with other download format options). Missing Data Identifiers: * In the BCO-DMO data system missing data identifiers are displayed according to the format of data you access. For example, in csv files it will be blank (null) values. In Matlab .mat files it will be NaN values. When viewing data online at BCO-DMO, the missing value will be shown as blank (null) values. * Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores.  Can not start with a number] * Local Date_PT column was converted to ISO 8601 format. (no time zone change). * Column DateTime renamed &quot;DateID_PT&quot; for consistency with other datasets in the project. * After consulting with the data submitter, an issue was corrected with minutes mismatching between time and datetime fields. Some DateID_PT values had minutes as :01 where they all should have been :00. Example correction: DateID_PT &quot;5/28/2021 17:01&quot; -&gt; &quot;5/28/2021 17:00&quot; (matching Time_PT 17:00). * Additional column ISO_DateTime_UTC added from Date and Time (local US/Pacific timezone) columns. Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact HDPE bottles HDPE bottles PI Supplied Instrument Name: HDPE bottles Instrument Name: High density polyethylene water bottle Instrument Short Name:HDPE bottle Instrument Description: A high density polyethylene (HDPE) water bottle. Often used for surface sampling from small boats. HDPE has a somewhat higher chemical resistance than low density polyethylene (LDPE). HDPE is also somewhat harder and more opaque and it can withstand higher temperatures (120 degrees Celsius for short periods, 110 degrees Celsius continuously).