http://lod.bco-dmo.org/id/dataset/984699
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-09-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
C and N stable isotope data for invertebrates collected by HOV Alvin during R/V Atlantis cruise AT50-24 at Sanak Seep Alaska from May 16 to June 5, 2024
2025-09-23
publication
2025-09-23
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-09-23
publication
https://doi.org/10.26008/1912/bco-dmo.984699.1
Lisa A. Levin
University of California-San Diego Scripps
principalInvestigator
María Emilia Emilia Bravo
Instituto Argentino de Oceanografía
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Pereira, O. S., Levin, L. A., Bravo, M. E. E. (2025) C and N stable isotope data for invertebrates collected by HOV Alvin during R/V Atlantis cruise AT50-24 at Sanak Seep Alaska from May 16 to June 5, 2024. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-09-23 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.984699.1 [access date]
Invertebrate stable isotopes from Alaskan seeps (May-June 2024) Dataset Description: Methods and Sampling: <p>Pushcore, rocks, biotubes, and slurp samples were collected with HOV Alvin using the manipulator. Rocks were placed into individual compartments in a biobox on the Alvin basket. Before preserving, samples were kept cold and animals were handpicked to sample tissue for stable isotope analyses.&nbsp;The tissue (aprox. 0.5-2.5 mg) was placed in pre-weighted tin boats or combusted vials (500C overnight) and frozen at -70C. <!--StartFragment-->In&nbsp;addition to natural abundance analyses, some animals were placed into mylar bags with addition of labeled C and N and protected from oxygen and light for incubation experiments. The bags were kept sealed in the ship's cold room for 3-8 days, and animals were then processed following the same method as for natural abundance samples.<!--EndFragment-->&nbsp;Surface and bottom seawater was collected using a CTD/Rosette system and filtered through microglass fiber filters. Filters were frozen for further processing in the laboratory.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2048720 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2048720
completed
Lisa A. Levin
University of California-San Diego Scripps
858-534-3579
9500 Gilman Drive Mail Code: 0218
La Jolla
CA
92093-0218
USA
llevin@ucsd.edu
pointOfContact
María Emilia Emilia Bravo
Instituto Argentino de Oceanografía
mebravo@gl.fcen.uba.ar
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Type
Date
Alvin_Dive
Site
Depth
Latitude
Longitude
Navigation_Comment
Sample
Sample_ID
Habitat
Phylum
Taxon_ID
AphiaID
Accepted_names
Incubation_treatment
d13C
d15N
CTD/Rosette system
Oven dried
HOV Alvin
Isotope ratio mass spectrometer
Weighted
theme
None, User defined
sample type
date
dive_id
site
depth
latitude
longitude
comments
sample identification
site description
taxon
taxon_code
treatment
d13C
d15N
featureType
BCO-DMO Standard Parameters
CTD - profiler
Drying Oven
HOV Alvin
Isotope-ratio Mass Spectrometer
scale or balance
instrument
BCO-DMO Standard Instruments
AT50-24
service
Deployment Activity
Gulf of Alaska
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Redefining the footprint of deep ocean methane seepage for benthic ecosystems
https://osprey.bco-dmo.org/project/840220
Collaborative Research: Redefining the footprint of deep ocean methane seepage for benthic ecosystems
<p><em>NSF Award Abstract:</em><br />
This research examines the role of deep-sea organisms in determining the fate and footprint of methane, a potent greenhouse gas, on Pacific continental margins. The investigators are evaluating the deep ocean methanosphere defined by the microbial communities that consume methane and the animals that directly feed on or form symbioses with methane-consuming microbes. They are also investigating animal communities that gain energy indirectly from methane, as well as those that take advantage of carbonate rocks, the physical manifestation of methane consumption in seafloor sediments. The study of methane seeps in the deep waters of both Alaska (4400-5500 meters) and Southern California (450-1040 meters) is enabling comparisons of the methanosphere under different food-limitation and oxygen regimes. By applying diverse chemical, isotopic, microscopy, and genetic-based analyses to seep microbes and fauna, this study is advancing understanding of the contribution of methane to deep-sea biodiversity and ecosystem function, information that can inform management and conservation actions in US waters. In addition to training for graduate and undergraduate students at their home institutions, the investigators are collaborating with the Alaska Native Science and Engineering Program (ANSEP). They are recruiting Alaskan undergraduates to participate in the research, contributing to ANSEP's online resources that promote interaction between scientists and middle and high school students, and participating in ANSEP's annual residential Career Exploration in Marine Science programs to engage middle school students in learning about deep-sea ecosystems and the variety of career pathways available in marine related fields.</p>
<p>Microbial production and consumption of methane is dynamic and widespread along continental margins, and some animals within deep-sea methane seeps rely on the oxidation and sequestration of methane for nutrition. At the same time, understanding of methane-dependent processes and symbioses in the deep-sea environment is still rudimentary. The goals of this study are to 1) examine the diversity of animals involved in methane-based symbioses and heterotrophic consumption of methane-oxidizing microbes and how these symbioses extend the periphery of seeps, contributing to non-seep, continental slope food webs; and 2) determine whether carbonates on the seep periphery sustain active methanotrophic microbial assemblages, providing a localized food source or chemical fuel for thiotrophic symbioses, via anaerobic oxidation of methane, or free-living, sulfide-oxidizing bacteria consumed by animals. The investigators are addressing these goals by surveying, sampling, and characterizing microbes, water, sediments, carbonates and animals at a deep seep site on the Aleutian Margin and a shallow site off Southern California. Shipboard experiments and laboratory analyses are using molecular, isotopic, geochemical, and radiotracer tools to understand transfer of methane-sourced carbon from aerobic methanotrophs under multiple oxygen levels, pressures, and photosynthetic food inputs. This approach offers a wide lens by which to examine the methane seep footprint, allow reinterpretation of past observations, and identify new scientific areas for future study. Improved characterization of the deep continental margin methanosphere informs climate science, biodiversity conservation, and resource management.</p>
Methanosphere
largerWorkCitation
project
eng; USA
biota
oceans
Gulf of Alaska
-162.5896971
-147.9997833
53.747843
57.4561925
2024-05-16
2024-06-05
Gulf of Alaska and Southern California Bight
0
BCO-DMO catalogue of parameters from C and N stable isotope data for invertebrates collected by HOV Alvin during R/V Atlantis cruise AT50-24 at Sanak Seep Alaska from May 16 to June 5, 2024
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/984730.rdf
Name: Type
Units: unitless
Description: <p>Whether the sample is for natural abundance or from the incubation experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/984731.rdf
Name: Date
Units: unitless
Description: <p>Date of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/984732.rdf
Name: Alvin_Dive
Units: unitless
Description: <p>Alvin Dive ID of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/984733.rdf
Name: Site
Units: unitless
Description: <p>Methane seep site of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/984734.rdf
Name: Depth
Units: meters
Description: <p>Depth of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/984735.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of sample collection, north is Positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/984736.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of sample collection, negative is West</p>
http://lod.bco-dmo.org/id/dataset-parameter/984737.rdf
Name: Navigation_Comment
Units: unitless
Description: <p>Note on missing navigation value</p>
http://lod.bco-dmo.org/id/dataset-parameter/984738.rdf
Name: Sample
Units: unitless
Description: <p>Type of sample (rock, pushcore, bio tube, or other) and number</p>
http://lod.bco-dmo.org/id/dataset-parameter/984739.rdf
Name: Sample_ID
Units: unitless
Description: <p>Sample unique identifier consisting of alvin dive number, sample type, and sample number</p>
http://lod.bco-dmo.org/id/dataset-parameter/984740.rdf
Name: Habitat
Units: unitless
Description: <p>Habitat where sample was collected (active seep, clam bed, tubeworm bed, seep periphery, etc.)</p>
http://lod.bco-dmo.org/id/dataset-parameter/984741.rdf
Name: Phylum
Units: unitless
Description: <p>Phylum of the specimen</p>
http://lod.bco-dmo.org/id/dataset-parameter/984742.rdf
Name: Taxon_ID
Units: unitless
Description: <p>Identification at the lowest taxonomic level possible of specimen</p>
http://lod.bco-dmo.org/id/dataset-parameter/984743.rdf
Name: AphiaID
Units: unitless
Description: <p>AphiaID of the lowest, accepted taxonomic level of specimen; blank if not animal</p>
http://lod.bco-dmo.org/id/dataset-parameter/984744.rdf
Name: Accepted_names
Units: unitless
Description: <p>Lowest, accepted taxonomic level of specimen; blank if not animal</p>
http://lod.bco-dmo.org/id/dataset-parameter/984745.rdf
Name: Incubation_treatment
Units: unitless
Description: <p>Incubation treatment described as "Natural abundance" or an incubation treatment with labeled C and/or N of incubation: "13C bicarbonate_N2", "13C-bicarb + 13NH4+", "13C-bicarb + 15NO3-", "13C-ethane + 15NH4 Air", "13CH4 + 15N2 Air", "13CH4 + 15NH4+ Air", "13CH4 + 15NO3- Air", "13CH4 only"</p>
http://lod.bco-dmo.org/id/dataset-parameter/984746.rdf
Name: d13C
Units: ‰ (per mil)
Description: <p>C isotope value</p>
http://lod.bco-dmo.org/id/dataset-parameter/984747.rdf
Name: d15N
Units: ‰ (per mil)
Description: <p>N isotope value</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
175444
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/eeedc705-e7cd-4642-b9dd-9ca6490584fa/content
download
https://doi.org/10.26008/1912/bco-dmo.984699.1
download
onLine
dataset
<p>Pushcore, rocks, biotubes, and slurp samples were collected with HOV Alvin using the manipulator. Rocks were placed into individual compartments in a biobox on the Alvin basket. Before preserving, samples were kept cold and animals were handpicked to sample tissue for stable isotope analyses.&nbsp;The tissue (aprox. 0.5-2.5 mg) was placed in pre-weighted tin boats or combusted vials (500C overnight) and frozen at -70C. <!--StartFragment-->In&nbsp;addition to natural abundance analyses, some animals were placed into mylar bags with addition of labeled C and N and protected from oxygen and light for incubation experiments. The bags were kept sealed in the ship's cold room for 3-8 days, and animals were then processed following the same method as for natural abundance samples.<!--EndFragment-->&nbsp;Surface and bottom seawater was collected using a CTD/Rosette system and filtered through microglass fiber filters. Filters were frozen for further processing in the laboratory.</p>
Specified by the Principal Investigator(s)
<p>In the laboratory, tissue samples were oven dried (60C) overnight, weighed and acidified with 12.5-25 ul 1N H3PO4 to remove inorganic C. Stable isotopes measurements (d13C and d15N) were carried out using an isotope ratio mass spectrometer at the Stable Isotope Facility at University of California, Davis. Microglass fiber filters were oven dried (60C) overnight, and the material on the filters was scrapped off and place into pre-weighed tin boats. Samples were then&nbsp;processed following the same method as for tissue samples.</p>
Specified by the Principal Investigator(s)
- Imported "AT50-24_Isotope data_BCO-DMO.xlsx" into the BCO-DMO system without formatting
- Applied the rounding formats that existed in the Excel document
- Converted the "Date (2024)" field to YYYY-MM-DD ISO 8601 format and renamed the field to "Date"
- Removed text "open all dive" and "not logged" from the navigation fields and put them into a new "Navigation_Comment" field
- Removed "not an animal" value from the from the dataset
- Corrected "-118.0000333" with "-148.00003" in the "Longitude" parameter, upon request of submitter
- Renamed fields to remove spaces and special characters to conform to BCO-DMO naming conventions
- Exported file as "984699_v1_at50-24_isotope_data.csv"
Aphia IDs were checked and provided by submitter upon submission (2025-07-01).
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
CTD/Rosette system
CTD/Rosette system
PI Supplied Instrument Name: CTD/Rosette system PI Supplied Instrument Description:Surface and bottom seawater was collected using a CTD/Rosette system and filtered through microglass fiber filters. Filters were frozen for further processing in the laboratory. Instrument Name: CTD - profiler Instrument Short Name: Instrument Description: The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.
This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
Oven dried
Oven dried
PI Supplied Instrument Name: Oven dried PI Supplied Instrument Description:In the laboratory, tissue samples were oven dried (60C) overnight, weighed and acidified with 12.5-25 ul 1N H3PO4 to remove inorganic C. Instrument Name: Drying Oven Instrument Short Name: Instrument Description: a heated chamber for drying
HOV Alvin
HOV Alvin
PI Supplied Instrument Name: HOV Alvin PI Supplied Instrument Description:Puschore, rocks, biotubes, and slurp samples were collected with HOV Alvin using the manipulator. Instrument Name: HOV Alvin Instrument Short Name: Instrument Description: Human Occupied Vehicle (HOV) Alvin is part of the National Deep Submergence Facility (NDSF). Alvin enables in-situ data collection and observation by two scientists to depths reaching 6,500 meters, during dives lasting up to ten hours.
Commissioned in 1964 as one of the world’s first deep-ocean submersibles, Alvin has remained state-of-the-art as a result of numerous overhauls and upgrades made over its lifetime. The most recent upgrades, begun in 2011 and completed in 2021, saw the installation of a new, larger personnel sphere with a more ergonomic interior; improved visibility and overlapping fields of view; longer bottoms times; new lighting and high-definition imaging systems; improved sensors, data acquisition and download speed. It also doubled the science basket payload, and improved the command-and-control system allowing greater speed, range and maneuverability.
With seven reversible thrusters, it can hover in the water, maneuver over rugged topography, or rest on the sea floor. It can collect data throughout the water column, produce a variety of maps and perform photographic surveys. Alvin also has two robotic arms that can manipulate instruments, obtain samples, and its basket can be reconfigured daily based on the needs of the upcoming dive.
Alvin's depth rating of 6,500m gives researchers in-person access to 99% of the ocean floor. Alvin is a proven and reliable platform capable of diving for up to 30 days in a row before requiring a single scheduled maintenance day. Recent collaborations with autonomous vehicles such as Sentry have proven extremely beneficial, allowing PIs to visit promising sites to collect samples and data in person within hours of their being discovered, and UNOLs driven technological advances have improved the ability for scientific outreach and collaboration via telepresence
Alvin is named for Allyn Vine, a WHOI engineer and geophysicist who helped pioneer deep submergence research and technology.
(from https://www.whoi.edu/what-we-do/explore/underwater-vehicles/hov-alvin/, accessed 2022-09-09)
Isotope ratio mass spectrometer
Isotope ratio mass spectrometer
PI Supplied Instrument Name: Isotope ratio mass spectrometer PI Supplied Instrument Description:Stable isotopes measurements (d13C and d15N) were carried out using an isotope ratio mass spectrometer at the Stable Isotope Facility at University of California, Davis. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Weighted
Weighted
PI Supplied Instrument Name: Weighted PI Supplied Instrument Description:In the laboratory, tissue samples were oven dried (60C) overnight, weighed and acidified with 12.5-25 ul 1N H3PO4 to remove inorganic C. Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/
Cruise: AT50-24
AT50-24
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT50-24
Lisa A. Levin
University of California-San Diego Scripps
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel