http://lod.bco-dmo.org/id/dataset/985777
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-10-02
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Polysaccharide hydrolysis rates from mesocosm and bulk water incubations from waters taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June 2022
2025-10-02
publication
2025-10-02
revision
BCO-DMO Linked Data URI
2025-10-02
creation
http://lod.bco-dmo.org/id/dataset/985777
Carol Arnosti
University of North Carolina at Chapel Hill
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lloyd, C., Brown, S. A., Ghobrial, S., Arnosti, C. (2025) Polysaccharide hydrolysis rates from mesocosm and bulk water incubations from waters taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-10-02 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/985777 [access date]
EN683 LV and Bulk FLA Rates Dataset Description: Methods and Sampling: <p>Seawater was collected aboard R/V Endeavor during the research cruise EN683 (2022-05-24 to 2022-06-12).&nbsp;Water samples were taken at the depth of the deep chlorophyll maximum (determined via CTD; ca 35m and 152m, respectively) and at the bottom, 4092m&nbsp; and 5305 m, respectively. The following stations were sampled in the Western North Atlantic:</p>
<ul>
<li>Station 21&nbsp;at approximately 33&nbsp;degrees&nbsp;44&nbsp; N, 75 degrees 17 W</li>
<li>Station&nbsp;22&nbsp;at approximately 42&nbsp;degrees&nbsp;52&nbsp; N, 53 degrees 52 W</li>
<li>Station&nbsp;23 at approximately 34 degrees 20 N, 69 degrees 49 W</li>
</ul>
<p>Water was collected via Niskin bottles mounted on a rosette, equipped with a CTD.&nbsp;</p>
<p>Seawater was transferred to 20 L carboys that were rinsed three times with water from the sampling depth and then filled with seawater from a single Niskin bottle, using silicone tubing that had been acid washed then rinsed with distilled water prior to use. From each carboy, water was dispensed into smaller glass containers that were cleaned and pre-rinsed three times with water from the carboy prior to dispensing. This water was used to measure cell counts, bacterial productivity, and the activities of polysaccharide hydrolases, peptidases, and glucosidases. A separate glass Duran bottle was filled with seawater from the carboy and sterilized in an autoclave for 20-30 minutes to serve as a killed control for microbial activity measurements.</p>
<p>For mesocosm (large volume) incubation experiments (referred to as “LV” incubations), seawater was transferred to 20 L carboys that were rinsed three times with water from the sampling depth and then filled with seawater from a single Niskin bottle, using silicone tubing that had been acid washed then rinsed with distilled water prior to use. At stations 22 and 23, carboys were filled from bottom water and deep chlorophyll maximum (DCM) water each, according to the CTD. Triplicate 20L carboys (a total of 9 carboys) were amended with high molecular weight material isolated from the diatom <em>Thalassiosira</em>, or the polysaccharide fucoidan, or the polysaccharide arabinogalactan; additional unamended single carboys were used for controls. From each carboy, water was dispensed into smaller glass containers that were cleaned and pre-rinsed three times with water from the carboy prior to dispensing. This water was used to measure the activities of peptidases, and glucosidases. A separate glass Duran bottle was filled with seawater from the carboy and sterilized in an autoclave to serve as a killed control for microbial activity measurements. All mesocosms were incubated in the dark at near in-situ temperatures. Mesocosms were sub-sampled for polysaccharide hydrolase activity measurements at the start of incubation (0 days), and then after at approximately 5d, 10d, 15d, and 25d.</p>
<p>Polysaccharide hydrolase activity was measured at each sub-sampling by filling three 50 mL falcon tubes with mesocosm incubated seawater and one 50 mL falcon tube was filled with autoclaved mesocosm incubated seawater to serve as a killed control, for each substrate. &nbsp; Polysaccharide substrate was added at 3.5 μM monomer-equivalent concentrations, except for fucoidan, which was added at 5 μM concentrations (a higher concentration was necessary for sufficient fluorescence signal). Two 50 mL falcon tubes – one with seawater and one with autoclaved seawater – with no added substrate served as blank controls. Incubations were stored in the dark at as close to in situ temperature as possible.</p>
<p>Subsamples of the incubations were collected at time zero, and at a sequence of subsequent time points. At each time point, 2 mL of seawater was collected from the 50 mL falcon tube using a sterile syringe, filtered through a 0.2 μm pore size syringe filter, and stored frozen until processing.</p>
<p>The hydrolysis of high molecular weight substrate to lower molecular weight hydrolysis products was measured using gel permeation chromatography with fluorescence detection, after the method of Arnosti (1996, 2003). In short, the subsample was injected onto a series of columns consisting of a 21 cm column of G50 and a 19 cm column of G75 Sephadex gel. The fluorescence of the column effluent was measured at excitation and emission wavelengths of 490 and 530 nm, respectively.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2022952 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2022952
onGoing
Carol Arnosti
University of North Carolina at Chapel Hill
919-962-5754
Dept. of Marine Sciences 3117A Venable/Murray Hall
Chapel Hill
NC
27599-3300
USA
arnosti@email.unc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
deployment
Station
latitude
longitude
date
time
ISO_DateTime_UTC
cast_number
depth_actual
sample_type
Incubation_Temp
unamended_amended
Sub_sample_day
substrate
rate_x
rate_1
rate_2
rate_3
mean_rate
sd_rate
kcrate_x
kcrate_1
kcrate_2
kcrate_3
mean_kcrate
sd_kcrate
CTD
HPLC system with Hitachi fluorescence detectors (L-7485, L-2485, Chromaster - 5440)
Niskin bottles
theme
None, User defined
Cruise identfier
station
latitude
longitude
date
time of day
ISO_DateTime_UTC
cast
depth
sample type
incubation or cultivation temperature (not ambient water temp)
treatment
time_point
hydrolase activity
mean
standard deviation
featureType
BCO-DMO Standard Parameters
CTD Sea-Bird SBE 911plus
High-Performance Liquid Chromatograph
Incubator
Niskin bottle
instrument
BCO-DMO Standard Instruments
EN683
service
Deployment Activity
Northern Atlantic
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Substrate structural complexity and abundance control distinct mechanisms of microbially-driven carbon cycling in the ocean
https://osprey.bco-dmo.org/project/896822
Substrate structural complexity and abundance control distinct mechanisms of microbially-driven carbon cycling in the ocean
<p>Substrate Structural Complexity and Abundance Control Distinct Mechanisms of Microbially-Driven Carbon Cycling in the Ocean</p>
<p>Almost half of the organic carbon produced in the ocean is processed by bacteria. Bacteria use extracellular (outside the cell) enzymes to break down large organic molecules to small sizes that can be transported into their cells. It has recently been discovered that bacteria use extracellular enzymes in two ways: ‘selfish uptake’ and ‘external hydrolysis’. External hydrolysis releases low molecular weight products to the environment where they can be used by other organisms. ‘Selfish uptake’ releases little or no products. This research will determine the extent and location of ‘selfish uptake’ in ocean waters. This process affects the distribution of organic carbon in the ocean, the flow of small organic molecules to feed a wider range of bacteria, and the composition and dynamics of the bacterial community. Recent results show that ‘selfish’ bacteria are active in deep ocean waters, where they take up complex polysaccharides (sugars) that are not hydrolyzed externally. These results inspired a new model that links ‘selfish uptake’ and external hydrolysis to the amount and complexity of the organic matter that is used by bacteria. This project will test the model by describing the polysaccharide fraction of marine organic matter, and studying the relationships between organic matter abundance, structural complexity, and extracellular enzyme use. Graduate and undergraduate students will participate in the project as members of the research team in the field and in the laboratory.</p>
<p>This research will test the hypothesis that the mechanism of polysaccharide processing is related to the cost to a cell of producing the enzymes required for its hydrolysis, and the probability that a cell will receive sufficient return on investment for producing the enzymes. The conceptual model that will be tested suggests that external hydrolysis is favored when organic matter is abundant, or when enzyme production costs can be shared (e.g., on particles, in biofilms); selfish uptake would be a better strategy when high molecular weight (HMW) organic matter is scarce, and particularly when the HMW organic matter is very complex. This study will test this model by characterizing the structure of polysaccharide-containing components of dissolved organic matter (DOM) and particulate organic matter (POM) collected from the ocean, by determining the extent of selfish uptake and rates of external hydrolysis of different polysaccharides by natural microbial communities from the surface and the deep ocean, and by incubation experiments that control for the abundance of polysaccharides of different structural complexity. This project will be carried out in collaboration with colleagues at the Max Planck Institute for Marine Microbiology, whose expertise in carbohydrate chemistry and structural analyses, and in advanced microscopy and analysis of complex microbial communities, are central to the project.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Substrate complexity and microbes
largerWorkCitation
project
eng; USA
biota
oceans
Northern Atlantic
-75.296666
-53.873585
33.7441666
42.870093
2022-05-24
2022-06-09
Western North Atlantic
0
BCO-DMO catalogue of parameters from Polysaccharide hydrolysis rates from mesocosm and bulk water incubations from waters taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June 2022
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/985833.rdf
Name: deployment
Units: unitless
Description: <p>Cruise ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/985834.rdf
Name: Station
Units: unitless
Description: <p>Station number 21, 22, or 23</p>
http://lod.bco-dmo.org/id/dataset-parameter/985835.rdf
Name: latitude
Units: Decimal degrees
Description: <p>Latitude of sampling site, South is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/985837.rdf
Name: longitude
Units: Decimal degrees
Description: <p>Longitude of sampling site, West is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/985839.rdf
Name: date
Units: unitless
Description: <p>Date of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/985840.rdf
Name: time
Units: unitless
Description: <p>Time of sample collection, US Eastern Time (ET)</p>
http://lod.bco-dmo.org/id/dataset-parameter/985841.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>DateTime of sample collection in ISO format in GMT/UTC</p>
http://lod.bco-dmo.org/id/dataset-parameter/985843.rdf
Name: cast_number
Units: unitless
Description: <p>Cast number (refers to cast of CTD/Niskin bottles on cruise)</p>
http://lod.bco-dmo.org/id/dataset-parameter/985844.rdf
Name: depth_actual
Units: m
Description: <p>Actual depth at which water was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/985846.rdf
Name: sample_type
Units: unitless
Description: <p>Sample from bulk water (bulk) or Large Volume incubation (LV)</p>
http://lod.bco-dmo.org/id/dataset-parameter/985847.rdf
Name: Incubation_Temp
Units: degrees Celsius
Description: <p>Temperature of incubation. RT = Room Temperature (~20 C)</p>
http://lod.bco-dmo.org/id/dataset-parameter/985848.rdf
Name: unamended_amended
Units: unitless
Description: <p>Whether high molecular weight organic matter was added or not; U = unamended, F = fucoidan, A = arabinogalactan, T = <em>Thalassiosira</em> extract; the following number corresponds to amended incubation replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/985849.rdf
Name: Sub_sample_day
Units: Days
Description: <p>The amount of incubation time that has elapsed at each timepoint in days</p>
http://lod.bco-dmo.org/id/dataset-parameter/985850.rdf
Name: substrate
Units: unitless
Description: <p>Polysaccharide used for incubation: ara = arabinogalactan, chn = chondroitin sulfate, fuc = fucoidan, lam = laminarin, pul = pullulan, xyl = xylan</p>
http://lod.bco-dmo.org/id/dataset-parameter/985851.rdf
Name: rate_x
Units: nmol L-1 hr-1
Description: <p>The hydrolysis rate for the kill-control</p>
http://lod.bco-dmo.org/id/dataset-parameter/985852.rdf
Name: rate_1
Units: nmol L-1 hr-1
Description: <p>The hydrolysis rate for the first replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/985853.rdf
Name: rate_2
Units: nmol L-1 hr-1
Description: <p>The hydrolysis rate for the second replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/985854.rdf
Name: rate_3
Units: nmol L-1 hr-1
Description: <p>The hydrolysis rate for the third replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/985855.rdf
Name: mean_rate
Units: nmol L-1 hr-1
Description: <p>The average hydrolysis rate for all replicates</p>
http://lod.bco-dmo.org/id/dataset-parameter/985856.rdf
Name: sd_rate
Units: nmol L-1 hr-1
Description: <p>The standard deviation of the hydrolysis rates for all replicates</p>
http://lod.bco-dmo.org/id/dataset-parameter/985857.rdf
Name: kcrate_x
Units: nmol L-1 hr-1
Description: <p>The kill-corrected hydrolysis rate for the kill-control</p>
http://lod.bco-dmo.org/id/dataset-parameter/985858.rdf
Name: kcrate_1
Units: nmol L-1 hr-1
Description: <p>The kill-corrected hydrolysis rate for the first replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/985859.rdf
Name: kcrate_2
Units: nmol L-1 hr-1
Description: <p>The kill-corrected hydrolysis rate for the second replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/985860.rdf
Name: kcrate_3
Units: nmol L-1 hr-1
Description: <p>The kill-corrected hydrolysis rate for the third replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/985861.rdf
Name: mean_kcrate
Units: nmol L-1 hr-1
Description: <p>The average kill-corrected hydrolysis rate for all replicates</p>
http://lod.bco-dmo.org/id/dataset-parameter/985862.rdf
Name: sd_kcrate
Units: nmol L-1 hr-1
Description: <p>The standard deviation of the kill-corrected hydrolysis rates for all replicates</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/985777/data/download
download
onLine
dataset
<p>Seawater was collected aboard R/V Endeavor during the research cruise EN683 (2022-05-24 to 2022-06-12).&nbsp;Water samples were taken at the depth of the deep chlorophyll maximum (determined via CTD; ca 35m and 152m, respectively) and at the bottom, 4092m&nbsp; and 5305 m, respectively. The following stations were sampled in the Western North Atlantic:</p>
<ul>
<li>Station 21&nbsp;at approximately 33&nbsp;degrees&nbsp;44&nbsp; N, 75 degrees 17 W</li>
<li>Station&nbsp;22&nbsp;at approximately 42&nbsp;degrees&nbsp;52&nbsp; N, 53 degrees 52 W</li>
<li>Station&nbsp;23 at approximately 34 degrees 20 N, 69 degrees 49 W</li>
</ul>
<p>Water was collected via Niskin bottles mounted on a rosette, equipped with a CTD.&nbsp;</p>
<p>Seawater was transferred to 20 L carboys that were rinsed three times with water from the sampling depth and then filled with seawater from a single Niskin bottle, using silicone tubing that had been acid washed then rinsed with distilled water prior to use. From each carboy, water was dispensed into smaller glass containers that were cleaned and pre-rinsed three times with water from the carboy prior to dispensing. This water was used to measure cell counts, bacterial productivity, and the activities of polysaccharide hydrolases, peptidases, and glucosidases. A separate glass Duran bottle was filled with seawater from the carboy and sterilized in an autoclave for 20-30 minutes to serve as a killed control for microbial activity measurements.</p>
<p>For mesocosm (large volume) incubation experiments (referred to as “LV” incubations), seawater was transferred to 20 L carboys that were rinsed three times with water from the sampling depth and then filled with seawater from a single Niskin bottle, using silicone tubing that had been acid washed then rinsed with distilled water prior to use. At stations 22 and 23, carboys were filled from bottom water and deep chlorophyll maximum (DCM) water each, according to the CTD. Triplicate 20L carboys (a total of 9 carboys) were amended with high molecular weight material isolated from the diatom <em>Thalassiosira</em>, or the polysaccharide fucoidan, or the polysaccharide arabinogalactan; additional unamended single carboys were used for controls. From each carboy, water was dispensed into smaller glass containers that were cleaned and pre-rinsed three times with water from the carboy prior to dispensing. This water was used to measure the activities of peptidases, and glucosidases. A separate glass Duran bottle was filled with seawater from the carboy and sterilized in an autoclave to serve as a killed control for microbial activity measurements. All mesocosms were incubated in the dark at near in-situ temperatures. Mesocosms were sub-sampled for polysaccharide hydrolase activity measurements at the start of incubation (0 days), and then after at approximately 5d, 10d, 15d, and 25d.</p>
<p>Polysaccharide hydrolase activity was measured at each sub-sampling by filling three 50 mL falcon tubes with mesocosm incubated seawater and one 50 mL falcon tube was filled with autoclaved mesocosm incubated seawater to serve as a killed control, for each substrate. &nbsp; Polysaccharide substrate was added at 3.5 μM monomer-equivalent concentrations, except for fucoidan, which was added at 5 μM concentrations (a higher concentration was necessary for sufficient fluorescence signal). Two 50 mL falcon tubes – one with seawater and one with autoclaved seawater – with no added substrate served as blank controls. Incubations were stored in the dark at as close to in situ temperature as possible.</p>
<p>Subsamples of the incubations were collected at time zero, and at a sequence of subsequent time points. At each time point, 2 mL of seawater was collected from the 50 mL falcon tube using a sterile syringe, filtered through a 0.2 μm pore size syringe filter, and stored frozen until processing.</p>
<p>The hydrolysis of high molecular weight substrate to lower molecular weight hydrolysis products was measured using gel permeation chromatography with fluorescence detection, after the method of Arnosti (1996, 2003). In short, the subsample was injected onto a series of columns consisting of a 21 cm column of G50 and a 19 cm column of G75 Sephadex gel. The fluorescence of the column effluent was measured at excitation and emission wavelengths of 490 and 530 nm, respectively.</p>
Specified by the Principal Investigator(s)
<p>Hydrolysis rates were calculated from the change in molecular weight distribution of the substrate over time, as described in detail in Arnosti (2003). Scripts to calculate hydrolysis rates are available in the associated Github repository (Hoarfrost, 2017).</p>
Specified by the Principal Investigator(s)
- Imported "FLA_Rates_EN683_LV_and_Bulk.csv" into the BCO-DMO system
- Replaced periods with underscores in parameter names
- Removed units from parameter names
- Replaced "2022-05-22" with "2022-05-24" in the "date" column, upon submitter request
- Added "Z" to the end of the UTC datetime field
- Exported file as "985777_v1_fla_rates_en683_lv_and_bulk.csv"
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
CTD
CTD
PI Supplied Instrument Name: CTD PI Supplied Instrument Description:Water was collected via Niskin bottles mounted on a rosette, equipped with a CTD. Instrument Name: CTD Sea-Bird SBE 911plus Instrument Short Name:CTD SBE 911plus Instrument Description: The Sea-Bird SBE 911 plus is a type of CTD instrument package for continuous measurement of conductivity, temperature and pressure. The SBE 911 plus includes the SBE 9plus Underwater Unit and the SBE 11plus Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 plus and SBE 11 plus is called a SBE 911 plus. The SBE 9 plus uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 plus and SBE 4). The SBE 9 plus CTD can be configured with up to eight auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0058/
HPLC system with Hitachi fluorescence detectors (L-7485, L-2485, Chromaster - 5440)
HPLC system with Hitachi fluorescence detectors (L-7485, L-2485, Chromaster - 5440)
PI Supplied Instrument Name: HPLC system with Hitachi fluorescence detectors (L-7485, L-2485, Chromaster - 5440) PI Supplied Instrument Description:The hydrolysis of high molecular weight substrate to lower molecular weight hydrolysis products was measured using gel permeation chromatography with fluorescence detection, after the method of Arnosti [1996, 2003]. In short, the subsample was injected onto a series of columns consisting of a 21 cm column of G50 and a 19 cm column of G75 Sephadex gel. The fluorescence of the column effluent was measured at excitation and emission wavelengths of 490 and 530 nm, respectively. Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
PI Supplied Instrument Name: PI Supplied Instrument Description:Polysaccharide hydrolase activity was measured at each sub-sampling by filling three 50 mL falcon tubes with mesocosm incubated seawater and one 50 mL falcon tube was filled with autoclaved mesocosm incubated seawater to serve as a killed control, for each substrate. Polysaccharide substrate was added at 3.5 μM monomer-equivalent concentrations, except for fucoidan, which was added at 5 μM concentrations (a higher concentration was necessary for sufficient fluorescence signal). Two 50 mL falcon tubes – one with seawater and one with autoclaved seawater – with no added substrate served as blank controls. Incubations were stored in the dark at as close to in situ temperature as possible. Instrument Name: Incubator Instrument Short Name:incubator (general) Instrument Description: A device in which environmental conditions (light, photoperiod, temperature, humidity, etc.) can be controlled.
Note: we have more specific terms for shipboard incubators (https://www.bco-dmo.org/instrument/629001) and in-situ incubators (https://www.bco-dmo.org/instrument/494).
Niskin bottles
Niskin bottles
PI Supplied Instrument Name: Niskin bottles PI Supplied Instrument Description:Water was collected via Niskin bottles mounted on a rosette, equipped with a CTD. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: EN683
EN683
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN683
Carol Arnosti
University of North Carolina at Chapel Hill
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel