http://lod.bco-dmo.org/id/dataset/986238
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-10-07
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Artemia nauplii consumption by Americamysis bahia as a function of body length from a pilot laboratory experiment
2025-10-07
publication
2025-10-07
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-10-15
publication
https://doi.org/10.26008/1912/bco-dmo.986238.1
Ryan J. Woodland
University of Maryland Center for Environmental Science
principalInvestigator
Theresa E. Murphy
University of Maryland Center for Environmental Science
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Santos, N. R., Murphy, T. E., Collins, L. D., Woodland, R. J. (2025) Artemia nauplii consumption by Americamysis bahia as a function of body length from a pilot laboratory experiment. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-10-07 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.986238.1 [access date]
Mysid consumption pilot test Dataset Description: Methods and Sampling: <p>Adult <em>Americamysis bahia </em>(hereafter, mysids) were purchased and shipped from Biosystems, Inc (Fort Collins, CO). Mysids were shipped acclimated to 15 ppt water salinity. Upon arrival at Chesapeake Biological Laboratory, mysids were acclimated to the experimental conditions of water temperature = 22 degrees C, and salinity = 14 ppt.&nbsp;Mysids were held for a minimum of 3 days prior to experimentation, during which period they were fed 1 day old <em>Artemia </em>nauplii <em>ad libitum</em>.&nbsp;</p>
<p>Prior to experiments, mysids were fasted for 1 day to allow depuration of stomach contents and ensure mysids were actively searching for prey. During experiments, individual mysids were placed in a 1-L container with filtered, artificial seawater (Instant Ocean® Sea Salt, Blacksburg, VA) mixed to a concentration of 14 ppt. A total of 60 (Series 1) or 40 (Series 2)&nbsp;1-day old <em>Artemia </em>nauplii were added to each container, sealed and placed on a slowly rotating,&nbsp;custom-made zooplankton grazing wheel. The wheel rotates very slowly around a central axis and is designed to keep plankton or zooplankton in suspension during grazing experiments. The experiment continued for 4 hours. Following each experiment, mysids placed in a petri dish and digitally measured for body length (rostrum to base of the telson) under light microscopy (CellSens&nbsp;imaging program, Evident Scientific).&nbsp;Containers were filtered using 40-micron nitex mesh and all remaining<em> Artemia </em>were enumerated.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2023349 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2023349
completed
Ryan J. Woodland
University of Maryland Center for Environmental Science
410-326-7332
146 Williams St, PO Box 38
Solomons
MD
20688-0038
USA
woodland@umces.edu
pointOfContact
Theresa E. Murphy
University of Maryland Center for Environmental Science
tmurphy@umces.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Series
ID
Mysid_length
Initial_artemia_count
Final_artemia_count
Artemia_consumed
microscopy
theme
None, User defined
sample identification
length
count
featureType
BCO-DMO Standard Parameters
Microscope - Optical
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Planktonic Omnivores and Stable Isotopes: Developing, Validating and Field-testing a Multi-species Functional Response Model
https://osprey.bco-dmo.org/project/826300
Planktonic Omnivores and Stable Isotopes: Developing, Validating and Field-testing a Multi-species Functional Response Model
<p>NSF Award Abstract:</p>
<p>Diagrams of food webs are typically drawn as boxes that show linkages between predators and prey. While these are useful models of how energy is transferred along a food chain, real food webs are more complex. Predator diets are often variable making it difficult to establish predator-prey links in marine communities. This project is investigating prey switching in a key member of coastal food webs, the shrimp-like mysid, Neomysis americana. Prey switching affects community structure and an organism’s resilience to environmental perturbation, but it is not easy to quantify. This project is using a combination of laboratory experiments and field sampling to develop a food web model that predicts mysid feeding patterns in the environment. This realistic and predictive food web model uses traditional gut analysis and analytical techniques that follow carbon and nitrogen as it is incorporated into the bodies of the mysids. In addition, mysid food preferences are being determined in the laboratory across a full range of diet possibilities. The calibrated gut analysis and chemical marker data in combination with feeding experiments are incorporated into the model, which then predicts mysid feeding on mixed diets under different environmental conditions. These predictions are validated against field data. Broader impacts include benefits to society for a better understanding of how coastal food webs work. Doctoral students and undergraduate students are being trained in experimental and field research. Increasing diversity in STEM fields is occurring through a partnership with two community colleges (College of Southern Maryland, Chesapeake College) to recruit summer interns for research experiences. Outreach activities include the development of educational materials for grade-appropriate hands-on laboratory experiments and training opportunities for middle and high school teacher groups in the use of these materials in their classrooms.</p>
<p>This project is developing and field-testing a generalizable approach to understand and predict complex predator-prey relationships in marine food webs. The research plan involves building and validating a multispecies functional response (MSFR) model for an omnivorous consumer, the mysid Neomysis americana. These models predict diet for consumers that feed on multiple types of prey under differing prey concentrations and identify conditions under which prey switching occurs in the environment. Recent and time-integrated diet tracking with gut contents, bulk stable isotope (SI) and compound-specific amino acid stable isotope (AA-CSI) analysis are validated in the lab and used to reconstruct diet of Neomysis in the field. The proposed research is testing specific hypotheses about Neomysis’ consumption rates and prey preferences and the effectiveness of integrating SI and AA-CSI into MSFR models. Laboratory experiments are determining prey-specific functional response curves by Neomysis under varying prey concentrations and environmental (temperature) conditions using grazing experiments. Experimental results are incorporated into a temperature-dependent MSFR model for a 5-compartment simplified food web (Neomysis, adult copepod, copepod nauplii, phytoplankton, detritus). A complementary element of the project is the experimental determination of bulk SI (ð¿13C and ð¿15N) and AA-CSI (ð¿15N) equilibration rates and trophic enrichment factors for Neomysis and each prey type. The predator-prey dynamics of Neomysis in the environment are being modeled using the lab-validated MSFR approach and field data, including prey concentrations, gut contents, and prey and Neomysis SI and AA-CSI data.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
MSFR
largerWorkCitation
project
eng; USA
biota
oceans
2023-11-03
2023-11-06
Chesapeake Bay
0
BCO-DMO catalogue of parameters from Artemia nauplii consumption by Americamysis bahia as a function of body length from a pilot laboratory experiment
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/986808.rdf
Name: Series
Units: unitless
Description: <p>Indicates the series during the sample was measured; Series 1 received 60 1-day old <em>Artemia </em>nauplii, while Series 2 received 40 1-day old <em>Artemia </em>nauplii</p>
http://lod.bco-dmo.org/id/dataset-parameter/986809.rdf
Name: ID
Units: unitless
Description: <p>Unique identifier given to each mysid</p>
http://lod.bco-dmo.org/id/dataset-parameter/986810.rdf
Name: Mysid_length
Units: mm
Description: <p>Rostrum to base of telson; length of each mysid</p>
http://lod.bco-dmo.org/id/dataset-parameter/986811.rdf
Name: Initial_artemia_count
Units: unitless
Description: <p>Starting number of <em>Artemia </em>nauplii in each chamber</p>
http://lod.bco-dmo.org/id/dataset-parameter/986812.rdf
Name: Final_artemia_count
Units: unitless
Description: <p>Ending number of <em>Artemia </em>nauplii in each chamber</p>
http://lod.bco-dmo.org/id/dataset-parameter/986813.rdf
Name: Artemia_consumed
Units: unitless
Description: <p>Calculated number of <em>Artemia </em>nauplii consumed during the experiment in each chamber</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
428
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/db9fa3c4-ae65-4664-ab9e-d8908f0e4055/content
download
https://doi.org/10.26008/1912/bco-dmo.986238.1
download
onLine
dataset
<p>Adult <em>Americamysis bahia </em>(hereafter, mysids) were purchased and shipped from Biosystems, Inc (Fort Collins, CO). Mysids were shipped acclimated to 15 ppt water salinity. Upon arrival at Chesapeake Biological Laboratory, mysids were acclimated to the experimental conditions of water temperature = 22 degrees C, and salinity = 14 ppt.&nbsp;Mysids were held for a minimum of 3 days prior to experimentation, during which period they were fed 1 day old <em>Artemia </em>nauplii <em>ad libitum</em>.&nbsp;</p>
<p>Prior to experiments, mysids were fasted for 1 day to allow depuration of stomach contents and ensure mysids were actively searching for prey. During experiments, individual mysids were placed in a 1-L container with filtered, artificial seawater (Instant Ocean® Sea Salt, Blacksburg, VA) mixed to a concentration of 14 ppt. A total of 60 (Series 1) or 40 (Series 2)&nbsp;1-day old <em>Artemia </em>nauplii were added to each container, sealed and placed on a slowly rotating,&nbsp;custom-made zooplankton grazing wheel. The wheel rotates very slowly around a central axis and is designed to keep plankton or zooplankton in suspension during grazing experiments. The experiment continued for 4 hours. Following each experiment, mysids placed in a petri dish and digitally measured for body length (rostrum to base of the telson) under light microscopy (CellSens&nbsp;imaging program, Evident Scientific).&nbsp;Containers were filtered using 40-micron nitex mesh and all remaining<em> Artemia </em>were enumerated.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Microsoft excel was used to record and organize data, associated metadata, and create the plot attached as supplemental file.&nbsp;</p>
Specified by the Principal Investigator(s)
- Imported "20231108_Mysidsizeexperiments_22C_1mysid_Americamysis.xlsx" sheet 2 into the BCO-DMO system
- Added "Series 1" and "Series 2" values to a new parameter "Series" to differentiate the sample numbers
- Removed spaces and special characters from the parameter names, in compliance with BCO-DMO standards
- Exported file as "986238_v1_mysid_consumption.csv"
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
microscopy
microscopy
PI Supplied Instrument Name: microscopy PI Supplied Instrument Description:Mysids were placed in a petri dish and digitally measured under light microscopy (CellSens imaging program, Evident Scientific). Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/