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            <gco:CharacterString>Cite this dataset as: Kelly Rakow Sutherland, Robert K. Cowen, Su Sponaugle, Elizabeth Wallace (2025) Carbon and nitrogen stable isotope measurements of gelatinous zooplankton and larval fish in the Northern California Current from March 2022 to August 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-12-01 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.986609.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Carbon and nitrogen stable isotopes in gelatinous zooplankton and larval fish Dataset Description:  Methods and Sampling: &amp;lt;p&amp;gt;Gelatinous zooplankton and larval fish were collected using MOCNESS (Multiple Opening/Closing Net and Environmental Sensing System) tows in 25-meter (m) depth increments in the upper 100m of the water column (nets: 1 square meter (m²) with 333-micrometer (µm) mesh and 4 m² with 1000 µm mesh). Organisms in each tow were grouped by lowest visually identifiable taxon. Maximum and minimum lengths of individuals in each group were measured with a ruler and the volume of each sample of grouped individuals was measured by displacement. Samples were frozen at -20 degrees Celsius (°C) prior to preparation for stable isotope analysis.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Wet weight of each grouped sample was measured upon thawing the sample. Samples were dried at 60°C, then dry weights were measured. Samples were then crushed and homogenized using an agate mortar and pestle. Stable isotope compositions and carbon and nitrogen contents were measured by elemental analyzer-isotope ratio mass spectrometry (EA-IRMS) at the UC Davis Stable Isotope Facility. Ash-free dry weights were determined by weighing samples before and after combusting dried samples at 500°C.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/855250.rdf" xlink:title="OCE-2125408" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2125408 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2125408</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/858394.rdf" xlink:title="OCE-2125407" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2125407 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2125407</gmx:Anchor>
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Marine plankton form the base of most ocean food webs that support valuable fisheries. This highly diverse and complex community is composed of organisms that drift with ocean currents. Planktonic organisms remain understudied: they are difficult to sample given that their sizes span more than six orders of magnitude from less than one micron to meters. Yet, understanding how these communities respond to climate change, and ultimately how these responses affect valuable fisheries, and therefore food security, is critical. Because many ecological and physiological processes are dictated by relative size, the theory of size spectra (i.e., the relationship between size and organism abundance as it drives ecosystem properties such as food webs) provides a valuable framework for forecasting climate change impacts on marine ecosystems. A deeper understanding of the scope and nature of variability in size spectra under contrasting environmental conditions is needed. The dynamic, highly productive northern California Current off Oregon and Washington, during the summer and winter seasons, produces a patchwork of oceanographic conditions including those associated with hypoxia and ocean acidification. This study is sampling the plankton communities in this region to investigate how gradients of temperature, nutrients, dissolved oxygen, and pH conditions impact size spectra. The broader impacts include the training of students, building scientific resources, and outreach to broader communities. Undergraduate and graduate students are being trained in oceanography, field research and new technologies. The automated image analysis pipeline developed as part of the project is openly accessible to the oceanographic community and the image data are available through the novel Global Plankton Imagery Library, an open-access repository for plankton imagery. Size spectra data from this study are shared directly with ecosystem modelers. The project’s flagship outreach activity is the collaboration with the Sitka Center for Art and Ecology and the hosting of an Artist-At-Sea Program. A professional artist is competitively selected to join the research cruises and to create artistic products that give a unique voice to oceanographic research and the organisms under study. The artwork is being assembled into a traveling public Art Exhibit with planned displays at the Sitka Center, Oregon State University’s Hatfield Marine Science Center, University of Oregon’s Charleston Marine Life Center and centers located in underserved coastal communities. Finally, imagery data from the project are being shared via the Plankton Portal, a public website developed in partnership with the Citizen Science Alliance’s Zooniverse, that invites citizen scientists to participate in classifying plankton images.&lt;/p&gt;
&lt;p&gt;The coupling of in situ plankton imagery and morphometric data allows quantifying scales of variation in plankton size spectra as well as testing predictions of how changes in environmental conditions (notably, temperature, nutrients, oxygen, pH) correlate with shifts in size spectra to reveal functional consequences to the food web. Plankton size spectra are being compared across environmental conditions by sampling in a habitat with steep environmental gradients and during two contrasting seasons. Planktonic organisms spanning 10 orders of magnitude in biomass are sampled using two complementary high-resolution imaging systems: the In Situ Ichthyoplankton Imaging System (ISIIS) and the Laser In-Situ Scattering and Transmissometry (LISST) particle imager. High-throughput image analysis software is used to create size distributions together with taxonomic classification. Depth-discrete meso-zooplankton samples are collected in parallel to examine community shifts in carbon, obtain length-to-carbon conversions and calibrate image data. The normalized biomass size spectra computed from the image data are tested for deviations from expected patterns. The plankton collections are also being analyzed for diet and reproductive status of gelatinous zooplankton, and diet and daily growth rate of representative larval fishes. These two groups have been historically understudied yet play central roles in ecosystem function. The data are being used to examine how these organisms are impacted by environmental conditions, and how they affect plankton size spectra. This study is foundational to the understanding of marine ecosystems within the context of climate change.&lt;/p&gt;
&lt;p&gt;This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.&lt;/p&gt;</gco:CharacterString>
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	Name: animal_type
	Units: unitless
	Description: &lt;p&gt;Organism type; grouped as Fish, Gelatinous zooplankton, or Octopus&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989259.rdf
	Name: phylum
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http://lod.bco-dmo.org/id/dataset-parameter/989260.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/989261.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/989262.rdf
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	Description: &lt;p&gt;Family (left blank if not identified to family level)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989263.rdf
	Name: genus
	Units: unitless
	Description: &lt;p&gt;Genus (left blank if not identified to genus level)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989264.rdf
	Name: species
	Units: unitless
	Description: &lt;p&gt;Species (left blank if not identified to species level)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989265.rdf
	Name: sample_full
	Units: unitless
	Description: &lt;p&gt;Unique sample name for each grouped organism sample. (###-seasonyear transectstation taxon)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989266.rdf
	Name: d13C
	Units: per mil vs. VPDB
	Description: &lt;p&gt;Carbon stable isotope composition (‰ vs. VPDB). Calibrated against international standards and corrected for instrument linearity and drift using in-house reference materials by UCD SIF. StDev of reference materials 0.10‰, StDev of gelatinous zooplankton standards 0.169‰.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989267.rdf
	Name: C_ug
	Units: micrograms (µg)
	Description: &lt;p&gt;Carbon content in measured sample of dried powdered tissue, calculated from IRMS peak area.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989268.rdf
	Name: d15N
	Units: per mil vs. Air
	Description: &lt;p&gt;Nitrogen stable isotope composition (‰ vs. Air). Calibrated against international standards and corrected for instrument linearity and drift using in-house reference materials by UCD SIF. StDev of reference materials 0.15‰, StDev of gelatinous zooplankton standards, 0.374‰.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989269.rdf
	Name: N_ug
	Units: micrograms (µg)
	Description: &lt;p&gt;Nitrogen content in measured sample of dried powdered tissue, calculated from IRMS peak area.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989270.rdf
	Name: sample_weight_mg
	Units: milligrams (mg)
	Description: &lt;p&gt;Weight of dried powder sample tissue analyzed for d13C, d15N, C contents, and N contents&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989271.rdf
	Name: season
	Units: unitless
	Description: &lt;p&gt;Season when samples were collected (Winter=Feb/March, Summer=July/Aug)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989272.rdf
	Name: cruise
	Units: unitless
	Description: &lt;p&gt;Cruise identifier: W22=Winter 2022, S22=Summer 2022, W23=Winter 2023, S23=Summer 2023&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989273.rdf
	Name: year
	Units: unitless
	Description: &lt;p&gt;Year when samples were collected&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989274.rdf
	Name: collection_date
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	Description: &lt;p&gt;Date when samples were collected&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989275.rdf
	Name: transect
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	Description: &lt;p&gt;Sampling transect identifier: GH=Grays Harbor, CR=Columbia River, CM=Cape Meares, NH= Newport hydrographic ,HH=Heceta Head, RR= Rogue River&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989276.rdf
	Name: station
	Units: unitless
	Description: &lt;p&gt;Location along transect: 1=closet nearshore; 6=furthest offshore&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989277.rdf
	Name: lat
	Units: decimal degrees
	Description: &lt;p&gt;Latitude of net tow start location&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989278.rdf
	Name: long
	Units: decimal degrees
	Description: &lt;p&gt;Longitude of net tow start location&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989279.rdf
	Name: moc
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	Description: &lt;p&gt;Net type: 1=Moc1, 1m2 opening 333um mesh; 4=Moc4, 4m2 opening 1000um mesh&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989280.rdf
	Name: collection_depth_m_min
	Units: meters (m)
	Description: &lt;p&gt;Minimum depth of range sampled by the MOCNESS net&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989281.rdf
	Name: collection_depth_m_max
	Units: meters (m)
	Description: &lt;p&gt;Maximum depth of range sampled by the MOCNESS net&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989282.rdf
	Name: life_stage
	Units: unitless
	Description: &lt;p&gt;Life/development stage of organism(s) in sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989283.rdf
	Name: number_of_individuals
	Units: individual organisms
	Description: &lt;p&gt;Number of individual organisms included in the grouped sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989284.rdf
	Name: length_min_mm
	Units: millimeters (mm)
	Description: &lt;p&gt;Minimum length of individual organisms in each grouped sample (bell diameter for medusae)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989285.rdf
	Name: length_max_mm
	Units: millimeters (mm)
	Description: &lt;p&gt;Maximum length of individual organisms in each grouped sample (bell diameter for medusae)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989286.rdf
	Name: vol_ml
	Units: milliliters (mL)
	Description: &lt;p&gt;Total volume of all organisms in grouped sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989287.rdf
	Name: notes
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http://lod.bco-dmo.org/id/dataset-parameter/989288.rdf
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	Description: &lt;p&gt;Total weight of all organisms in grouped sample upon thawing&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989289.rdf
	Name: dry_weight_g
	Units: grams (g)
	Description: &lt;p&gt;Total weight of all organisms in grouped sample after drying at 60°C&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989290.rdf
	Name: afdw_perc_of_dw
	Units: percent (%)
	Description: &lt;p&gt;Ash-free dry weight as a percentage of the dry weight (material lost after combustion at 500°C)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/989291.rdf
	Name: ash_perc_of_dw
	Units: percent (%)
	Description: &lt;p&gt;Ash percentage of the dry weight (material retained after combustion at 500°C)&lt;/p&gt; 
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&amp;lt;p&amp;gt;Wet weight of each grouped sample was measured upon thawing the sample. Samples were dried at 60°C, then dry weights were measured. Samples were then crushed and homogenized using an agate mortar and pestle. Stable isotope compositions and carbon and nitrogen contents were measured by elemental analyzer-isotope ratio mass spectrometry (EA-IRMS) at the UC Davis Stable Isotope Facility. Ash-free dry weights were determined by weighing samples before and after combusting dried samples at 500°C.&amp;lt;/p&amp;gt;</gco:CharacterString>
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- Converted date column to YYYY-MM-DD format.
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                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/546339.rdf" xlink:title="Elemental Analyzer" xlink:actuate="onRequest">Sercon Europa ANCA-GSL elemental analyzer</gmx:Anchor>
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                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/546339.rdf" xlink:title="Elemental Analyzer" xlink:actuate="onRequest">Elementar vario MICRO cube elemental analyzer</gmx:Anchor>
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          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Elementar vario EL cube elemental analyzer PI Supplied Instrument Description:Elementar vario EL cube elemental analyzer interfaced to an Elementar VisION IRMS (Elementar Analysensysteme GmbH, Langenselbold, Germany): 527 samples were analyzed for C and N contents and stable isotope compositions using this EA-IRMS system. Instrument Name: Elementar Vario EL Cube elemental analyzer Instrument Short Name:Vario EL Cube   Instrument Description: A laboratory instrument used for quantifying organic elements. It can measure C, H, N and S and optionally O, Cl and TIC. It was first developed in 2006 as a successor to the vario EL III. It uses a high-temperature combustion unit that is able to complete sample digestion at up to 1200 deg C (or 1800 deg C at the point of combustion when tin foil is used) and a jet injection of oxygen directly to the sample during combustion. Separation of gas components are performed on up to 3 gas-selective columns which trap gases until they are heated up and the prior gas peak has reached the baseline during detection. It uses a Thermal Conductivity Detector (TCD) as standard. An infrared (IR) detector for sulfur and oxygen and electrochemical detector for chlorine are optionally available. The instrument can measure C / N elemental ratios of up to 12,000:1 and provides an elemental detection limit of &lt; 40 ppm (TCD).</gco:CharacterString>
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              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/469.rdf" xlink:title="Isotope-ratio Mass Spectrometer" xlink:actuate="onRequest">Sercon Europa 20-20 IRMS</gmx:Anchor>
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            <gco:CharacterString>Sercon Europa 20-20 IRMS</gco:CharacterString>
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          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Sercon Europa 20-20 IRMS PI Supplied Instrument Description:Sercon Europa ANCA-GSL elemental analyzer interfaced to a Sercon Europa 20-20 IRMS (Sercon Ltd., Cheshire, United Kingdom): 70 samples were analyzed for C and N contents and stable isotope compositions using this EA-IRMS system. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS   Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/</gco:CharacterString>
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              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/511.rdf" xlink:title="MOCNESS" xlink:actuate="onRequest">Multiple Opening/Closing Net and Environmental Sensing System (MOCNESS)</gmx:Anchor>
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        </gmi:MI_Instrument>
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      <gmi:instrument>
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              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/714.rdf" xlink:title="scale or balance" xlink:actuate="onRequest">Mettler Toledo MS105 Analytical Balance</gmx:Anchor>
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                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/714.rdf" xlink:title="scale or balance" xlink:actuate="onRequest">Mettler Toledo WXTS3DU Microbalance</gmx:Anchor>
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         <gmx:Anchor xlink:href="https://www.sikuliaq.alaska.edu/" xlink:actuate="onRequest">R/V Sikuliaq</gmx:Anchor>
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          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/561518.rdf"
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