http://lod.bco-dmo.org/id/dataset/986649
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-10-13
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Water quality metrics collected from reef water in the Flower Garden Banks National Marine Sanctuary in Mar 2023
2025-11-12
publication
2025-11-12
revision
Woods Hole Oceanographic Institution (WHOI BCO-DMO)
2025-12-03
publication
https://doi.org/10.26008/1912/bco-dmo.986649.1
Amy Apprill
Woods Hole Oceanographic Institution
principalInvestigator
Marilyn Brandt
University of the Virgin Islands Center for Marine and Environmental Studies
principalInvestigator
Adrienne M.S. Correa
Rice University
principalInvestigator
Daniel Holstein
Louisiana State University
principalInvestigator
Laura Mydlarz
University of Texas at Arlington
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Kastner, M., Correa, A. M., Mydlarz, L., Brandt, M., Holstein, D., Apprill, A. (2025) Water quality metrics collected from reef water in the Flower Garden Banks National Marine Sanctuary in Mar 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-11-12 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.986649.1 [access date]
Environmental data FGB March 2023 Dataset Description: Methods and Sampling: <p><strong>Collection</strong></p>
<p>At each sampling location, we collected reef seawater for water quality metrics: the microbial community of the surrounding environment, the total organic carbon and total nitrogen, and cell abundances. Water was collected via SCUBA 2 m above the reef. Samples were collected in acid-washed and seawater-rinsed bottles.</p>
<p>To quantify cell abundances of Prochlorococcus, Synechococcus, picoeukaryotes, and unpigmented (presumably heterotrophic bacteria/archaea) microorganisms, seawater (1.4 mL) was preserved using paraformaldehyde, and frozen in liquid nitrogen vapors. Water for non-purgeable organic carbon (TOC) and organic nitrogen was collected into combusted, borosilicate EPA vials and acidified with phosphoric acid, then stored at 4 degrees Celsius.</p>
<p>Sample processing will be conducted using previously published methods (Becker et al., 2020; Weber et al., 2020).&nbsp;</p>
<p><strong>Cell abundances</strong></p>
<p>Acid-washed and seawater-rinsed bottles (HDPE, Nalgene, Thermo - Fisher Scientific); &nbsp;2 ml cryovial (Corning); 1% paraformaldehyde (Electron Microscopy Sciences); Beckman-Coulter Altra flow cytometer (Beckman Coulter Life Sciences) that was attached to a Harvard Apparatus syringe pump and &nbsp;stained with Hoechst 33342 DNA stain (1 μg ml−1 final concentration) and excited co-linearly by 488 nm (1W) and UV (~350 nm, 200 mW) lasers (Becker et al., 2020).&nbsp;</p>
<p><strong>Total organic carbon and total nitrogen</strong></p>
<p>After preservation with phosphoric acid, TOC and TN concentrations will be determined using a Shimadzu TOC-VCSH total organic carbon analyzer equipped with a TNM-1 module at Woods Hole Oceanographic Institution. Results will be made available after&nbsp;they have been processed.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2316580 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2316580
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2316578 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2316578
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2316579 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2316579
completed
Amy Apprill
Woods Hole Oceanographic Institution
508-289-2649
Marine Chemistry and Geochemistry Department 266 Woods Hole Road, MS# 4
Woods Hole
MA
02543
USA
aapprill@whoi.edu
pointOfContact
Marilyn Brandt
University of the Virgin Islands Center for Marine and Environmental Studies
340-693-1379
2 John Brewers Bay
St. Thomas, USVI
00802
mbrandt@uvi.edu
pointOfContact
Adrienne M.S. Correa
Rice University
amsc@berkeley.edu
pointOfContact
Daniel Holstein
Louisiana State University
NY
USA
daniel.holstein@stonybrook.edu
pointOfContact
Laura Mydlarz
University of Texas at Arlington
817-272-0397
501 S. Nedderman Dr. 337 Life Sciences Building
Arlington
TX
76013
United States
mydlarz@uta.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Mission
Site
Sampling_datetime_UTC
Sampling_depth_ft
Sampling_depth_m
Lab_ID_for_cell_abundances
pro
syn
peuk
hbact
Lab_ID_for_TON_TOC
npoc
tn
Beckman-Coulter Altra flow cytometer
Shimadzu TOC-VCSH TOC analyser
theme
None, User defined
site description
site
ISO_DateTime_UTC
depth
sample identification
bacterial abundance
pico_euks
total organic Carbon
Nitrogen
featureType
BCO-DMO Standard Parameters
Flow Cytometer
Total Organic Carbon Analyzer
instrument
BCO-DMO Standard Instruments
PE23-18
service
Deployment Activity
Flower Garden Banks National Marine Sanctuary
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: RAPID: A multi-scale approach to predicting coral disease spread: leveraging an outbreak on coral-dense isolated reefs
https://osprey.bco-dmo.org/project/889917
Collaborative Research: RAPID: A multi-scale approach to predicting coral disease spread: leveraging an outbreak on coral-dense isolated reefs
<p><em>NSF Award Abstract:</em></p>
<p>Marine diseases have devastating impacts on ocean ecosystems and this work directly informs understanding of disease transmission in the ocean. To understand the cause and patterns of spread of a disease outbreak that began in late summer of 2022 at the Flower Garden Banks National Marine Sanctuary (northwest Gulf of Mexico, GoM), a team of ecologists, ocean connectivity and disease modelers, microbiologists, and coral immunologists (from Rice University, the University of Virgin Islands (UVI), Louisiana State University (LSU), and Woods Hole Oceanographic Institution) is monitoring the health of corals, and biopsy their tissues. This data aid in developing a model that predicts coral disease transmission and its impacts on economically valuable coral reefs in the GoM. This project supports multidisciplinary field and laboratory research experiences of graduate students at multiple minority-serving institutions, and provides undergraduate students with hands-on training in modeling, ecological and molecular analysis techniques. UVI and LSU are in EPSCoR jurisdictions and have diverse student bodies, including numerous under-represented minority (URM) students. The research team collaboratively provides URM students with research experiences in STEM fields. Project findings are being broadly communicated through virtual public programming, to the Disease Advisory Council, and via direct updates to managers of the Flower Garden Bank National Marine Sanctuary.</p>
<p>Over the last four decades, diseases decimated ecosystem engineers in marine coastal environments, including coral reefs. Recent results from studies of white plague and stony coral tissue loss disease (SCTLD) show coral species immune traits can influence disease resistance and therefore predict of coral community structure post-outbreak in the Caribbean. In late August of 2022, an unidentified multi-species acute tissue loss disease with signs and species susceptibility characteristics reminiscent of white plague or SCTLD was documented at the Flower Garden Banks (northwest Gulf of Mexico, GoM). This disease is having significant impacts on FGB and could become widespread across the GoM, offering an opportunity to test hypotheses about the influence of coral community composition and pathogen dispersal on disease spread during the early stages of an outbreak; few studies examine this on relatively isolated, deep, coral-dense reefs. The interdisciplinary research team employs photomosaics and colony fate-tracking, layered molecular datasets and microscopy approaches, as well as modeling of disease reservoirs and dispersal to assess the etiology of the disease and contribute to the development of a generalizable framework for disease spread on reefs. By parsing the impacts of reef-scale community composition versus seascape-scale dispersal in disease transmission and persistence, this work helps reveal the potential resistance and resilience of isolated, coral-dense reefs to diseases that decimate these ecosystems across the wider Caribbean.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
FGB Disease
largerWorkCitation
project
eng; USA
biota
oceans
Flower Garden Banks National Marine Sanctuary
-93.8148166667
-93.8148166667
27.8753166667
27.8753166667
2023-03-06
2023-03-11
Flower Garden Banks National Marine Sanctuary, northwest Gulf of Mexico
0
BCO-DMO catalogue of parameters from Water quality metrics collected from reef water in the Flower Garden Banks National Marine Sanctuary in Mar 2023
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/988693.rdf
Name: Mission
Units: unitless
Description: <p>Mission description</p>
http://lod.bco-dmo.org/id/dataset-parameter/988694.rdf
Name: Site
Units: unitless
Description: <p>Site number of sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/988695.rdf
Name: Sampling_datetime_UTC
Units: unitless
Description: <p>Date and time of sampling, UTC</p>
http://lod.bco-dmo.org/id/dataset-parameter/988697.rdf
Name: Sampling_depth_ft
Units: feet
Description: <p>Depth of sampling for non-Sonde samples in feet</p>
http://lod.bco-dmo.org/id/dataset-parameter/988698.rdf
Name: Sampling_depth_m
Units: meters
Description: <p>Depth of sampling for non-Sonde samples in meters, converted from ft</p>
http://lod.bco-dmo.org/id/dataset-parameter/988700.rdf
Name: Lab_ID_for_cell_abundances
Units: unitless
Description: <p>Sample IDs for Cell Abundances</p>
http://lod.bco-dmo.org/id/dataset-parameter/988701.rdf
Name: pro
Units: cells/ml
Description: <p>Prochlorococcus concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/988702.rdf
Name: syn
Units: cells/ml
Description: <p>Synechococcus concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/988703.rdf
Name: peuk
Units: cells/ml
Description: <p>Picoeukaryote concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/988704.rdf
Name: hbact
Units: cells/ml
Description: <p>Heterotrophic Bacteria and Archaea concentrations</p>
http://lod.bco-dmo.org/id/dataset-parameter/988705.rdf
Name: Lab_ID_for_TON_TOC
Units: unitless
Description: <p>Sample IDs for TON and TOC samples</p>
http://lod.bco-dmo.org/id/dataset-parameter/988706.rdf
Name: npoc
Units: uM
Description: <p>Total organic carbon concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/988707.rdf
Name: tn
Units: uM
Description: <p>Total nitrogen concentration</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://doi.org/10.26008/1912/bco-dmo.986649.1
download
onLine
dataset
<p><strong>Collection</strong></p>
<p>At each sampling location, we collected reef seawater for water quality metrics: the microbial community of the surrounding environment, the total organic carbon and total nitrogen, and cell abundances. Water was collected via SCUBA 2 m above the reef. Samples were collected in acid-washed and seawater-rinsed bottles.</p>
<p>To quantify cell abundances of Prochlorococcus, Synechococcus, picoeukaryotes, and unpigmented (presumably heterotrophic bacteria/archaea) microorganisms, seawater (1.4 mL) was preserved using paraformaldehyde, and frozen in liquid nitrogen vapors. Water for non-purgeable organic carbon (TOC) and organic nitrogen was collected into combusted, borosilicate EPA vials and acidified with phosphoric acid, then stored at 4 degrees Celsius.</p>
<p>Sample processing will be conducted using previously published methods (Becker et al., 2020; Weber et al., 2020).&nbsp;</p>
<p><strong>Cell abundances</strong></p>
<p>Acid-washed and seawater-rinsed bottles (HDPE, Nalgene, Thermo - Fisher Scientific); &nbsp;2 ml cryovial (Corning); 1% paraformaldehyde (Electron Microscopy Sciences); Beckman-Coulter Altra flow cytometer (Beckman Coulter Life Sciences) that was attached to a Harvard Apparatus syringe pump and &nbsp;stained with Hoechst 33342 DNA stain (1 μg ml−1 final concentration) and excited co-linearly by 488 nm (1W) and UV (~350 nm, 200 mW) lasers (Becker et al., 2020).&nbsp;</p>
<p><strong>Total organic carbon and total nitrogen</strong></p>
<p>After preservation with phosphoric acid, TOC and TN concentrations will be determined using a Shimadzu TOC-VCSH total organic carbon analyzer equipped with a TNM-1 module at Woods Hole Oceanographic Institution. Results will be made available after&nbsp;they have been processed.&nbsp;</p>
Specified by the Principal Investigator(s)
- Imported "EEID_WaterQuality_FGB2023_BCODMO.xlsx" into the BCO-DMO system
- Converted "date" (which included time) to the ISO 8601 date time format
- Removed " ft" string text from the "Sampling_depth" values to convert the parameter from string to number
- Replaced spaces and special characters and removed units in parameter names to comply with BCO-DMO guidelines
- Created a new field, ""Sampling_depth_m" with converted depth values in meters (units used to differentiate these fields with depth information)
- Renamed "Sampling_depth" to ""Sampling_depth_ft" (units used to differentiate these fields with depth information)
- Exported file as "986649_v1_water_quality_flower_garden_banks.csv"
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Beckman-Coulter Altra flow cytometer
Beckman-Coulter Altra flow cytometer
PI Supplied Instrument Name: Beckman-Coulter Altra flow cytometer PI Supplied Instrument Description:Cell abundances: Acid-washed and seawater-rinsed bottles (HDPE, Nalgene, Thermo - Fisher Scientific); 2 ml cryovial (Corning); 1% paraformaldehyde (Electron Microscopy Sciences); Beckman-Coulter Altra flow cytometer (Beckman Coulter Life Sciences) that was attached to a Harvard Apparatus syringe pump and stained with Hoechst 33342 DNA stain (1 μg ml−1 final concentration) and excited co-linearly by 488 nm (1W) and UV (~350 nm, 200 mW) lasers (Becker et al., 2020). Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
Shimadzu TOC-VCSH TOC analyser
Shimadzu TOC-VCSH TOC analyser
PI Supplied Instrument Name: Shimadzu TOC-VCSH TOC analyser PI Supplied Instrument Description:Total organic carbon and total nitrogen: concentrated phosphoric acid, Shimadzu TOC-VCSH TOC analyser. Instrument Name: Total Organic Carbon Analyzer Instrument Short Name:TOC analyzer Instrument Description: A unit that accurately determines the carbon concentrations of organic compounds typically by detecting and measuring its combustion product (CO2). See description document at: http://bcodata.whoi.edu/LaurentianGreatLakes_Chemistry/bs116.pdf Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
Cruise: PE23-18
PE23-18
R/V Pelican
Community Standard Description
International Council for the Exploration of the Sea
R/V Pelican
vessel
PE23-18
Adrienne M.S. Correa
Rice University
R/V Pelican
Community Standard Description
International Council for the Exploration of the Sea
R/V Pelican
vessel