http://lod.bco-dmo.org/id/dataset/986721
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-10-13
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Dissolved and total organic carbon concentrations of seawater collected in the northwestern Atlantic in 2024 during three cruises aboard the Atlantic Explorer (AE2412, AE2426b, AE2427)
2025-11-13
publication
2025-11-13
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-11-16
publication
https://doi.org/10.26008/1912/bco-dmo.986721.1
Krista Longnecker
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Longnecker, K. (2025) Dissolved and total organic carbon concentrations of seawater collected in the northwestern Atlantic in 2024 during three cruises aboard the Atlantic Explorer (AE2412, AE2426b, AE2427). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-11-13 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.986721.1 [access date]
DOC and TOC data in NW Atlantic Dataset Description: Methods and Sampling: <p>Seawater was collected from Niskin bottles or the ship’s underway system. Samples were processed to obtain the concentrations of dissolved organic carbon and total organic carbon. The seawater for total organic carbon (TOC) was aliquoted directly into a combusted glass vial. For dissolved organic carbon (DOC), seawater was filtered with a 0.2 µM Omnipore filter (Whatman) mounted in a Teflon filter holder into a combusted glass vial. Both types of samples were acidified to pH~3 with concentrated hydrochloric acid (HCl) and stored at 4°C until analysis with a Shimadzu TOC-L. Blanks (MilliQ water) and standard curves with potassium hydrogen phthalate were interspersed into the sample runs. The coefficient of variability between replicate injections was &lt;1%. Comparisons to standards provided by Prof. D. Hansell (University of Miami) were made daily.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2049004 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2049004
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2044346 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2044346
completed
Krista Longnecker
Woods Hole Oceanographic Institution
508-289-2824
266 Woods Hole Road MS# 04
Woods Hole
MA
02543
USA
klongnecker@whoi.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cruise
ISO_DateTime_UTC
Latitude
Longitude
Cast
Depth
Source
SampleType
NPOC
Niskin bottles
underway water system
Shimadzu TOC-L
theme
None, User defined
Cruise identifier
ISO_DateTime_UTC
latitude
longitude
cast
depth
sampling_method
sample type
organic carbon
featureType
BCO-DMO Standard Parameters
Niskin bottle
Pump - Surface Underway Ship Intake
Shimadzu TOC-L Analyzer
instrument
BCO-DMO Standard Instruments
AE2412
AE2426b
AE2427
service
Deployment Activity
BATS site (31 50 N, 64 10 W)
Woods Hole, MA to Bermuda
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Quantifying the role of microbial extracellular vesicles in marine dissolved organic matter production and consumption
https://osprey.bco-dmo.org/project/853399
Collaborative Research: Quantifying the role of microbial extracellular vesicles in marine dissolved organic matter production and consumption
<p><em>NSF Award Abstract:</em><br />
Microbial production and consumption of organic carbon play critical roles in the marine food web and global carbon cycling. Bacteria release organic matter in a variety of chemical forms and in diverse contexts, ranging from individual molecules to small aggregates and larger biological particles. In recent years we have come to understand that most, if not all, marine microbes release nanoscale structures called extracellular vesicles from their surfaces. These discrete particles, which are abundant in the oceans, are capable of transporting multiple classes of organic molecules between organisms and can serve as a potential nutrient source for other microbes. Extracellular vesicles thus represent a potentially important component of marine microbial food webs, but the magnitude and dynamics of this contribution are unknown. Further, the packaging of material within vesicles may influence the accessibility of this organic material as compared with truly ‘dissolved’ substances to different groups of marine organisms, potentially biasing nutrient exchanges. Broader impacts of this work is providing hands-on research experiences for female undergraduate students - including those from groups historically underrepresented in STEM fields - and training in data analysis tools.</p>
<p>The goal of this project is to advance the understanding of the role that extracellular vesicles play in marine dissolved organic carbon pools and microbial food webs. To determine the contribution of vesicles to organic matter release by marine microbes, the investigators are quantifying the fraction of excreted carbon and nitrogen associated with vesicles released by multiple marine cyanobacteria and heterotrophs. The project is examining how vesicles are ‘consumed’ by heterotrophs to calculate a mass balance of vesicle utilization and produce detailed gene expression data to explore how cells respond to the presence of vesicles. Finally, experiments with coastal and oligotrophic marine communities are providing insights into which organisms utilize vesicles in the field, and whether they are broadly accessible to all microbes or are instead preferentially consumed by a subset of microbes. Collectively, these experiments are opening up a new area of research into the mechanisms underlying the microbial loop and provide foundational insights into the roles of extracellular vesicles in ocean ecosystems.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
VesicleDOM
largerWorkCitation
project
eng; USA
oceans
BATS site (31 50 N, 64 10 W); Woods Hole, MA to Bermuda
-70.734167
-64.00283
31.01117
40.67267
2024-04-06
2024-11-25
Laboratory; Vineyard Sound, MA, USA; Bermuda Atlantic Time Series Station
0
BCO-DMO catalogue of parameters from Dissolved and total organic carbon concentrations of seawater collected in the northwestern Atlantic in 2024 during three cruises aboard the Atlantic Explorer (AE2412, AE2426b, AE2427)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/988807.rdf
Name: Cruise
Units: dimensionless
Description: <p>Cruise number on R/V Atlantic Explorer</p>
http://lod.bco-dmo.org/id/dataset-parameter/988808.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and Time of sampling in GMT (UTC) time zone</p>
http://lod.bco-dmo.org/id/dataset-parameter/988810.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/988812.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/988814.rdf
Name: Cast
Units: dimensionless
Description: <p>Consecutive cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/988815.rdf
Name: Depth
Units: meters (m)
Description: <p>Depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/988817.rdf
Name: Source
Units: dimensionless
Description: <p>Sampling method used for water collection, either 'profile' or 'underway'</p>
http://lod.bco-dmo.org/id/dataset-parameter/988818.rdf
Name: SampleType
Units: dimensionless
Description: <p>Non-purgeable organic carbon sample type, either 'DOC' = filtered seawater, or 'TOC' = whole seawater</p>
http://lod.bco-dmo.org/id/dataset-parameter/988819.rdf
Name: NPOC
Units: micromolar (uM)
Description: <p>Non-purgeable organic carbon measurement</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
4147
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/0e0b9f61-affb-403a-b3c8-6b2790c6d9ab/content
download
https://doi.org/10.26008/1912/bco-dmo.986721.1
download
onLine
dataset
<p>Seawater was collected from Niskin bottles or the ship’s underway system. Samples were processed to obtain the concentrations of dissolved organic carbon and total organic carbon. The seawater for total organic carbon (TOC) was aliquoted directly into a combusted glass vial. For dissolved organic carbon (DOC), seawater was filtered with a 0.2 µM Omnipore filter (Whatman) mounted in a Teflon filter holder into a combusted glass vial. Both types of samples were acidified to pH~3 with concentrated hydrochloric acid (HCl) and stored at 4°C until analysis with a Shimadzu TOC-L. Blanks (MilliQ water) and standard curves with potassium hydrogen phthalate were interspersed into the sample runs. The coefficient of variability between replicate injections was &lt;1%. Comparisons to standards provided by Prof. D. Hansell (University of Miami) were made daily.</p>
Specified by the Principal Investigator(s)
- Imported data from source file "Longnecker_NPOCdata.2025.08.20.xlsx" into the BCO-DMO data system.
- Combined the date and time columns into a single ISO datetime UTC value
- Combined the latitude degrees and latitude minutes into a single latitude in decimal degrees
- Combined the longitude degrees and longitude minutes into a single longitude in decimal degrees
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Niskin bottles
Niskin bottles
PI Supplied Instrument Name: Niskin bottles PI Supplied Instrument Description:Seawater was collected from Niskin bottles or the ship’s underway system. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
underway water system
underway water system
PI Supplied Instrument Name: underway water system PI Supplied Instrument Description:Seawater was collected from Niskin bottles or the ship’s underway system. Instrument Name: Pump - Surface Underway Ship Intake Instrument Short Name:Pump-Ship Intake Instrument Description: The 'Pump-underway ship intake' system indicates that samples are from the ship's clean water intake pump. This is essentially a surface water sample from a source of uncontaminated near-surface (commonly 3 to 7 m) seawater that can be pumped continuously to shipboard laboratories on research vessels. There is typically a temperature sensor near the intake (known as the hull temperature) to provide measurements that are as close as possible to the ambient water temperature. The flow from the supply is typically directed through continuously logged sensors such as a thermosalinograph and a fluorometer. Water samples are often collected from the underway supply that may also be referred to as the non-toxic supply. Ideally the data contributor has specified the depth in the ship's hull at which the pump is mounted. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/31/
Shimadzu TOC-L
Shimadzu TOC-L
PI Supplied Instrument Name: Shimadzu TOC-L PI Supplied Instrument Description:Both DOC and TOC samples were measured with a Shimadzu TOC-L analyzer. Instrument Name: Shimadzu TOC-L Analyzer Instrument Short Name:Shimadzu TOC-L Instrument Description: A Shimadzu TOC-L Analyzer measures DOC by high temperature combustion method.
Developed by Shimadzu, the 680 degree C combustion catalytic oxidation method is now used worldwide. One of its most important features is the capacity to efficiently oxidize hard-to-decompose organic compounds, including insoluble and macromolecular organic compounds. The 680 degree C combustion catalytic oxidation method has been adopted for the TOC-L series.
http://www.shimadzu.com/an/toc/lab/toc-l2.html Community Standard Description: http://onto.nerc.ac.uk/CAST/124.html
Cruise: AE2412
AE2412
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
AE2412
Claire Medley
Bermuda Institute of Ocean Sciences
Cruise: AE2426b
AE2426b
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
AE2426b
Krista Longnecker
Woods Hole Oceanographic Institution
Cruise: AE2427
AE2427
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
AE2427
Claire Medley
Bermuda Institute of Ocean Sciences
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel