14:I[71133,["4507","static/chunks/e2799680-446c489506865058.js","7665","static/chunks/7665-81c5407bc4489ff9.js","8500","static/chunks/8500-e4dd1cb1bb626827.js","9753","static/chunks/9753-028c0a26aa71f2d4.js","8437","static/chunks/8437-a09d0b83fcec76af.js","4557","static/chunks/4557-64c5efa221bc528f.js","947","static/chunks/947-410627998426400f.js","5357","static/chunks/5357-6142894e88cb7da2.js","8382","static/chunks/app/dataset/%5Bid%5D/data/view/page-177ea8adea7aed86.js"],"default"] 15:Tce6,

Sampling

Glycans were profiled in bulk and high molecular weight dissolved organic matter (HMWDOM) and particulate organic matter (POM) in the deep chlorophyll max (DCM) and bottom water at three stations in the western North Atlantic Ocean.

Sampling and processing of HMWDOM and POM

Seawater was collected using 30 L Niskin bottles on-board the R/V Endeavor. Niskin bottles were attached to rosettes equipped with conductivity, temperature and depth (CTD) sensors. Physical parameters were recorded using a Seabird 911+ CTD profiler. Data were processed and binned with SBE Data Processing software (v7.26.7). Collected seawater was transferred into acid-washed carboys before processing on-board. HMWDOM was concentrated to ~300 mL by tangential flow filtration (TFF). The TFF system (Sartorius <>) was run with 3 filter cassettes (1 kDa). HMWDOM samples were further concentrated back in the lab by freeze-drying and resuspension in lower volumes of MilliQ-water; for example, 10 mL of HMWDOM was freeze-dried and resuspended in 2.5 to 3mL. These concentrated HMWDOM samples were used for acid hydrolysis and microarray printing. POM samples were taken using in situ pumps onboard R/V Endeavor (EN683). At station 21 ~393 L were filtered in surface waters and 1276 L between 2800 and 3100 m depth. At station 22 ~214 L were sampled at DCM and 931 L at depths between 3321 and 3694 m. At station 23 the in situ pumps were deployed at 110 m (DCM) and 5100 m (depth) and pumped 515 L and 1196 L, respectively.

Sequential extraction of polysaccharides from GF/F filters

Seven punchouts of 11 m diameter were made from GF/F filters and sequentially extracted using MilliQ-water, 0.3M EDTA and 4M NaOH + 0.1% NaBH4 (Vidal-Melgosa et al., 2021). Subsequently solvents were added to the filter pieces, vortexed and incubated for 2 h at 60°C at 650 rpm in a heat block. Extracts were centrifuged at 6,000 x g for 15 min at 15°C and the supernatant was transferred to a new tube. NaOH extracts were neutralized with 4M HCl.

Quantification of monosaccharides

Polysaccharides of HMWDOM and POM samples were hydrolysed into monosaccharides. Freeze dried and concentrated HMWDOM samples (500 µL) were acid hydrolysed with 500 µL of 2M HCl. Additionally, 10 x 5 mm diameter punchouts from all GF/F filters were directly transferred to ampoules and acid hydrolysed in 750 µL 1M HCl. All hydrolyses were run at 100°C for 24 hrs. The supernatant of acid hydrolysed samples was dried in an acid-resistant vacuum concentrator (Martin Christ Gefriertrocknungsanlagen GmbH, Germany). Hydrolysates were reconstituted in MilliQ-water and pH adjusted to >7 with 0.1 M NaOH. Monosaccharide standards and hydrolysates were spiked with 13C-labelled glucose, galactose and mannose before derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) (Rühmann et al., 2014). PMP-derivatives were separated on a Agilent 1290 Infinity II LC system equipped with a Waters CORTECS UPLC C18 column and measured on a SCIEX qTRAP5500 by multiple reaction monitoring (MRM) (Xu et al., 2018). Signal intensities were normalised to 13-labelled standards and calibrated against standard curves.

16:T6db,This research tested the hypothesis that the mechanism of polysaccharide processing is related to the cost to a cell of producing the enzymes required for its hydrolysis, and the probability that a cell will receive sufficient return on investment for producing the enzymes. Our conceptual model suggests that external (extracellular) hydrolysis is favored when organic matter is abundant, or when enzyme production costs can be shared (e.g., on particles, in biofilms); selfish uptake (hydrolysis without production of low molecular weight products in the environment) would be a better strategy when high molecular weight (HMW) organic matter is scarce, and particularly when the HMW organic matter is very complex. We know that a substantial fraction of HMW dissolved organic matter consists of carbohydrates, including neutral sugars, and that its concentration is lower in the deep ocean than in the upper mesopelagic/surface ocean (Benner & Amon 2015). A key focus of this project's field work is investigating the potential of marine heterotrophic microbial communities from different water masses and under differing conditions of organic matter availability to hydrolyze six well characterized high-molecular-weight (HMW) polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan). To better contextualize this hydrolysis, bulk water characterization of the DCM and bottom waters used in our mesocosm incubation experiments was performed. This dataset includes the measurement of monosaccharide composition of POM-derived carbohydrates from bulk seawater and mesocosm experiments taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022.17:Tcb7,Substrate Structural Complexity and Abundance Control Distinct Mechanisms of Microbially-Driven Carbon Cycling in the Ocean Almost half of the organic carbon produced in the ocean is processed by bacteria. Bacteria use extracellular (outside the cell) enzymes to break down large organic molecules to small sizes that can be transported into their cells. It has recently been discovered that bacteria use extracellular enzymes in two ways: ‘selfish uptake’ and ‘external hydrolysis’. External hydrolysis releases low molecular weight products to the environment where they can be used by other organisms. ‘Selfish uptake’ releases little or no products. This research will determine the extent and location of ‘selfish uptake’ in ocean waters. This process affects the distribution of organic carbon in the ocean, the flow of small organic molecules to feed a wider range of bacteria, and the composition and dynamics of the bacterial community. Recent results show that ‘selfish’ bacteria are active in deep ocean waters, where they take up complex polysaccharides (sugars) that are not hydrolyzed externally. These results inspired a new model that links ‘selfish uptake’ and external hydrolysis to the amount and complexity of the organic matter that is used by bacteria. This project will test the model by describing the polysaccharide fraction of marine organic matter, and studying the relationships between organic matter abundance, structural complexity, and extracellular enzyme use. Graduate and undergraduate students will participate in the project as members of the research team in the field and in the laboratory. This research will test the hypothesis that the mechanism of polysaccharide processing is related to the cost to a cell of producing the enzymes required for its hydrolysis, and the probability that a cell will receive sufficient return on investment for producing the enzymes. The conceptual model that will be tested suggests that external hydrolysis is favored when organic matter is abundant, or when enzyme production costs can be shared (e.g., on particles, in biofilms); selfish uptake would be a better strategy when high molecular weight (HMW) organic matter is scarce, and particularly when the HMW organic matter is very complex. This study will test this model by characterizing the structure of polysaccharide-containing components of dissolved organic matter (DOM) and particulate organic matter (POM) collected from the ocean, by determining the extent of selfish uptake and rates of external hydrolysis of different polysaccharides by natural microbial communities from the surface and the deep ocean, and by incubation experiments that control for the abundance of polysaccharides of different structural complexity. This project will be carried out in collaboration with colleagues at the Max Planck Institute for Marine Microbiology, whose expertise in carbohydrate chemistry and structural analyses, and in advanced microscopy and analysis of complex microbial communities, are central to the project. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.6:["$","$L14",null,{"dataset":{"_id":"987279","description":"","problem":"","acquisition":"$15","processing":"

Data processed using Microsoft Excel.

\n","bcodmoProcessing":"- Imported \"20250925_EN683_Monosaccharides_DOM_POM_LV_BCODMO.csv\" into the BCO-DMO system\r\n- Combined \"date\" and \"time\" (local EST time) and converted the value into a UTC ISO datetime value\r\n- Renamed fields to comply with BCO-DMO naming guidelines, removing spaces, units, and parentheses\r\n- Exported file as \"987279_v1_en683_monosaccharides_dom_pom_lv.csv\"","title":"Monosaccharide Composition of HMWDOM concentrate and POM-derived Carbohydrates from bulk seawater and mesocosm experiments taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022","nickname":"ENEN683_Monosaccharides_DOM_POM_LV","dataUrl":"","currentState":"Final no updates expected","currentStateId":"7","validated":true,"restricted":false,"version":"1","version_date":"2025-10-16T04:00:00.000Z","abstract":"$16","citation":"Lloyd, C., Bligh, M., Arnosti, C., Ghobrial, S. (2026) Monosaccharide Composition of HMWDOM concentrate and POM-derived Carbohydrates from bulk seawater and mesocosm experiments taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-10-16 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.987279.1 [access date]","citationParts":{"identifier":"doi:10.26008/1912/bco-dmo.987279.1","versionDate":"2025-10-16","versionId":"1","year":"2026","title":"Monosaccharide Composition of HMWDOM concentrate and POM-derived Carbohydrates from bulk seawater and mesocosm experiments taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022","authors":"Lloyd, C., Bligh, M., Arnosti, C., Ghobrial, S.","publisher":"Biological and Chemical Oceanography Data Management Office (BCO-DMO)."},"doi":"10.26008/1912/bco-dmo.987279.1","release_date":null,"field_dataset_location":"Western North Atlantic, stations 21, 22, and 23. Water samples were taken at the depth of the deep chlorophyll maximum (determined via CTD; ca 35m and 152m, respectively) and at the bottom, 4092m and 5305 m, respectively.","temporal_extent":{"start":"2022-05-25T04:00:00.000Z","end":"2022-06-09T04:00:00.000Z"},"temporal":{"resolution":"time:unitDay","type":"time:Interval","start":{"value":"1653451200","label":"2022-05-25"},"end":{"value":"1654747200","label":"2022-06-09"}},"spatial_extent":{"shape":"polygon","center":{"latitude":38.47835,"longitude":-64.6817},"wkt":"POLYGON ((-75.4622 33.86, -53.9012 33.86, -53.9012 43.0967, -75.4622 43.0967, -75.4622 33.86))","north":43.0967,"east":-53.9012,"south":33.86,"west":-75.4622},"dataset_type":["Cruise Results"],"keywords":["oceans"],"data_files":[{"id":"1003628","title":"987279_v1_en683_monosaccharides_dom_pom_lv.csv","url":"https://datadocs.bco-dmo.org/dataset/987279/file/B1kyngGTR856jJ/987279_v1_en683_monosaccharides_dom_pom_lv.csv","showTableUrl":"https://www.bco-dmo.org/dataset/987279/data/view/1003628","description":"Primary data file for dataset ID 987279, version 1","filename":"987279_v1_en683_monosaccharides_dom_pom_lv.csv","bytesize":54531,"checksum":{"value":"40ea7a0d643eeba43f35e852002d39f5","Algorithm":{"_id":"190","name":"MD5"}},"mimetype":"text/csv","fileType":{"_id":"192","name":"Comma Separated Values (.csv)"},"isErddap":"","date":"","isPublicDuringEmbargo":false,"primaryDataFile":true,"showTable":true,"restricted":false}],"supplemental_files":[],"roles":[{"role":{"_id":"138","name":"Principal Investigator"},"performedBy":{"_id":"661940","name":"Carol Arnosti"},"performedAtOrganization":{"_id":"20","name":"University of North Carolina at Chapel Hill"}},{"role":{"_id":"128","name":"Student"},"performedBy":{"_id":"987281","name":"Margot Bligh"},"performedAtOrganization":{"_id":"287","name":"Max Planck Institute for Marine Microbiology"}},{"role":{"_id":"128","name":"Student"},"performedBy":{"_id":"956112","name":"Chad Lloyd"},"performedAtOrganization":{"_id":"20","name":"University of North Carolina at Chapel Hill"}},{"role":{"_id":"137","name":"Data Manager"},"performedBy":{"_id":"890958","name":"Sherif Ghobrial"},"performedAtOrganization":{"_id":"20","name":"University of North Carolina at Chapel Hill"}},{"role":{"_id":"145","name":"BCO-DMO Data Manager"},"performedBy":{"_id":"924467","name":"Audrey Mickle"},"performedAtOrganization":{"_id":"191","name":"Woods Hole Oceanographic Institution"}}],"deployments":[{"_id":"956118","name":"EN683","synonyms":[],"dates":{"start":"2022-05-24T00:00:00.000Z","end":"2022-06-12T00:00:00.000Z"},"platform":{"_id":"53992","name":"R/V Endeavor"},"chiefScientists":[{"id":"661940","name":"Carol Arnosti","role":"Chief Scientist"}]}],"funding":[{"_id":"896821","name":"OCE-2022952","fundingSource":{"_id":"355","name":"NSF Division of Ocean Sciences"},"url":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=2022952"}],"instruments":[{"_id":"988194","name":"Centrifuge","description":"Subsequently solvents were added to the filter pieces, vortexed and incubated for 2 h at 60°C at 650 rpm in a heat block. Extracts were centrifuged at 6,000 x g for 15 min at 15°C and the supernatant was transferred to a new tube. ","type_id":"629890","type_name":"Centrifuge","type_desc":"A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids.","type_acronym":""},{"_id":"987354","name":"Speed-vac","description":"After acid hydrolysis, the samples were dried on a speed-vac and resuspended in Milli-Q to remove any HCl. ","type_id":"847391","type_name":"Concentrator Device","type_desc":"A concentrator is a device designed to increase the weight per unit volume of a substance.\r\n\r\nThis category includes vacuum centrifuge concentrator, which include a vacuum chamber within which a centrifuge rotor is mounted for spinning a plurality of vials containing a solution at high speed while subjecting the solution to a vacuum condition for concentration and evaporation. Alternative names: sample concentrator; speed vacuum; speed vac.","type_acronym":""},{"_id":"987293","name":"CTD","description":"Niskin bottles were attached to rosettes equipped with conductivity, temperature and depth (CTD) sensors. Physical parameters were recorded using a Seabird 911+ CTD profiler. ","type_id":"591","type_name":"CTD Sea-Bird SBE 911plus","type_desc":"The Sea-Bird SBE 911 plus is a type of CTD instrument package for continuous measurement of conductivity, temperature and pressure. The SBE 911 plus includes the SBE 9plus Underwater Unit and the SBE 11plus Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 plus and SBE 11 plus is called a SBE 911 plus. The SBE 9 plus uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 plus and SBE 4). The SBE 9 plus CTD can be configured with up to eight auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). more information from Sea-Bird Electronics","type_acronym":"CTD SBE 911plus"},{"_id":"987291","name":"Elementar Analysensysteme","description":"POC analysis- Cario MICRO cube; Elementar Analysensysteme (elemental analyzer).","type_id":"546339","type_name":"Elemental Analyzer","type_desc":"Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material.","type_acronym":""},{"_id":"988195","name":"Incubator","description":"Subsequently solvents were added to the filter pieces, vortexed and incubated for 2 h at 60°C at 650 rpm in a heat block. Extracts were centrifuged at 6,000 x g for 15 min at 15°C and the supernatant was transferred to a new tube. ","type_id":"926396","type_name":"Incubator","type_desc":"A device in which environmental conditions (light, photoperiod, temperature, humidity, etc.) can be controlled.\r\n\r\nNote: we have more specific terms for shipboard incubators (https://www.bco-dmo.org/instrument/629001) and in-situ incubators (https://www.bco-dmo.org/instrument/494).","type_acronym":"incubator (general)"},{"_id":"987292","name":"Dionex ICS-5000+ system with pulsed amperometric detection (HPAEC-PAD)","description":"Monosaccharide composition of POM - Dionex ICS-5000+ system with pulsed amperometric detection (HPAEC-PAD) (anion exchange chromatography).","type_id":"662","type_name":"Ion Chromatograph","type_desc":"Ion chromatography is a form of liquid chromatography that measures concentrations of ionic species by separating them based on their interaction with a resin. Ionic species separate differently depending on species type and size. Ion chromatographs are able to measure concentrations of major anions, such as fluoride, chloride, nitrate, nitrite, and sulfate, as well as major cations such as lithium, sodium, ammonium, potassium, calcium, and magnesium in the parts-per-billion (ppb) range. From: http://serc.carleton.edu/microbelife/research_methods/biogeochemical/ic.html","type_acronym":"Ion Chromatograph"},{"_id":"987294","name":"Niskin bottles","description":"Niskin bottles were attached to rosettes equipped with conductivity, temperature and depth (CTD) sensors. Physical parameters were recorded using a Seabird 911+ CTD profiler. ","type_id":"413","type_name":"Niskin bottle","type_desc":"A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.","type_acronym":"Niskin bottle"},{"_id":"988196","name":"Vortex","description":"Subsequently solvents were added to the filter pieces, vortexed and incubated for 2 h at 60°C at 650 rpm in a heat block. Extracts were centrifuged at 6,000 x g for 15 min at 15°C and the supernatant was transferred to a new tube. ","type_id":"987728","type_name":"vortex mixer","type_desc":"A vortex mixer is an electrical rotator that blends or mixes substances or ingredients, in whirling or rotary motion, for homogenizing samples.","type_acronym":""}],"parameters":[{"_id":"987334","name":"deployment","format":"","frictionlessType":"string","description":"

Cruise ID on R/V Endeavor

","units":{"_id":"","name":"unitless"},"type":{"_id":"1102","name":"cruise_id","description":"

cruise designation; name

"}},{"_id":"987335","name":"station","format":"","frictionlessType":"integer","description":"

Cruise station number (21, 22, 23)

","units":{"_id":"","name":"unitless"},"type":{"_id":"987","name":"station","description":"

Station identifier; a unique number or alphanumeric string designating a general geographic location at which one or more sampling activities may occur.

"}},{"_id":"987336","name":"latitude","format":"","frictionlessType":"number","description":"

Latitude, south is negative

","units":{"_id":"","name":"decimal degrees"},"type":{"_id":"730","name":"latitude","description":"

latitude, in decimal degrees, North is positive, negative denotes South; Reported in some datasets as degrees, minutes

"}},{"_id":"987338","name":"longitude","format":"","frictionlessType":"number","description":"

Longitude, west is negative

","units":{"_id":"","name":"decimal degrees"},"type":{"_id":"731","name":"longitude","description":"

longitude, in decimal degrees, East is positive, negative denotes West; Reported in some datasets as degrees, minutes

"}},{"_id":"987340","name":"date","format":"%Y-%m-%d","frictionlessType":"date","description":"

Date of sample collection

","units":{"_id":"","name":"unitless"},"type":{"_id":"1070","name":"date","description":"

date; generally reported in GMT as YYYYMMDD (year; month; day); also as MMDD (month; day); EqPac dates are local Hawaii time. ISO_Date format is YYYY-MM-DD (http://www.iso.org/iso/home/standards/iso8601.htm)

"}},{"_id":"987341","name":"time","format":"%H:%M:%S","frictionlessType":"time","description":"

Time of sample collection, Eastern Time (ET)

","units":{"_id":"","name":"unitless"},"type":{"_id":"1075","name":"time","description":"

Time GMT defined as HHMM; time of day; generally reported in GMT as HHMM (hours and minutes) but may also be HH.H (decimal hours) or HHMMSS (hours; minutes and seconds); in EqPac time is reported as Hawaii local time

"}},{"_id":"987342","name":"ISO_DateTime_UTC","format":"%Y-%m-%dT%H:%M:%SZ","frictionlessType":"datetime","description":"

Datetime of collection in UTC

","units":{"_id":"","name":"unitless"},"type":{"_id":"1908","name":"ISO_DateTime_UTC","description":"

Date/Time (UTC) ISO formatted
\nThis standard is based on ISO 8601:2004(E) and takes on any of the following forms:
\n2009-08-30T09:05:00[.xx] (local time)
\n2009-08-30T14:05:00[.xx]Z (UTC time)
\n2009-08-30T14:05:00[.xx]-05:00
\nThe dashes and the colons can be dropped.
\nThe T can also be dropped \"by mutual agreement\", but one needs the trailing Z if the time is UTC.

Sample ISO_datetime_utc:
\n2009-08-30T14:05:00[.xx]Z (UTC time)

Format: YYYY-mm-ddTHH:MM:SS[.xx]Z (UTC time)

"}},{"_id":"987344","name":"depth_description","format":"","frictionlessType":"string","description":"

Sequence of depths sampled (d1 is surface; higher numbers at greater depths)

","units":{"_id":"","name":"unitless"},"type":{"_id":"1740","name":"site_descrip","description":"

Description or characteristics of a sampling site

"}},{"_id":"987345","name":"depth_actual","format":"","frictionlessType":"number","description":"

Actual depth at which water was collected

","units":{"_id":"","name":"meters"},"type":{"_id":"808","name":"depth","description":"

Observation/sample depth below the sea surface. Units often reported as: meters, feet.

\nWhen used in a JGOFS/GLOBEC dataset the depth is a best estimate; usually but not always calculated from pressure; calculated either from CTD pressure using Fofonoff and Millard (1982; UNESCO Tech Paper #44) algorithm adjusted for 1980 equation of state for seawater (EOS80) or simply equivalent to nominal depth as recorded during sampling if CTD pressure was unavailable.

"}},{"_id":"987347","name":"sample_type","format":"","frictionlessType":"string","description":"

HMWDOM = high molecular weight dissolved organic matter or POM = particulate organic matter

","units":{"_id":"","name":"unitless"},"type":{"_id":"1742","name":"sample_type","description":"

text description of type of sample collected (rock, bio, fluid, etc)

"}},{"_id":"987348","name":"amendment_type","format":"","frictionlessType":"string","description":"

Mesocosm was unamended (U), or amended with high molecular weight organic matter; F, A, T refer to type of organic matter added (Fucoidan, Arabinogalactan, Thalassiosira extract)

","units":{"_id":"","name":"unitless"},"type":{"_id":"489066","name":"treatment","description":"

Experimental conditions applied to experimental units. In comparative experiments, members of the complementary group, the control group, receive either no treatment or a standard treatment.

"}},{"_id":"987349","name":"Sub_sample_timepoint","format":"","frictionlessType":"integer","description":"

Subsample from mesocosm experiments. Blank values indicate it is not a subsample from a mesocosm experiment

","units":{"_id":"","name":"unitless"},"type":{"_id":"472026","name":"time_point","description":"

Arbitrary time point of sampling in a sampling sequence (T0, T1,....Tn)

"}},{"_id":"987350","name":"volume_filtered","format":"","frictionlessType":"number","description":"

The amount of seawater filtered (liters) for particulate organic carbon and monosaccharide composition analysis.

","units":{"_id":"","name":"Liters"},"type":{"_id":"1040","name":"vol_filt","description":"

volume of water filtered; in this form generally applies to the volume of water filtered during plankton tows; however; this parameter name is also used for a variety of non plankton sampling and the units can vary, e.g. US JGOFS EqPac Bishop MULVFS sampler vol_filt is reported in liters.

"}},{"_id":"987351","name":"concentrated_Volume","format":"","frictionlessType":"number","description":"

Final concentrated volume of sample

","units":{"_id":"","name":"mL"},"type":{"_id":"542214","name":"volume","description":"

The amount of 3-dimensional space an object occupies. Capacity.
\nAs in the volume of the tank or container used in a laboratory experiment.

"}},{"_id":"987352","name":"monosaccharide","format":"","frictionlessType":"string","description":"

The monosaccharide measured

","units":{"_id":"","name":"unitless"},"type":{"_id":"910593","name":"monosaccharide","description":"

Parent monosaccharides are polyhydroxy aldehydes H[CH(OH)]nC(=O)H or polyhydroxy ketones H‒[CHOH]n‒C(=O)[CHOH]m‒H with three or more carbon atoms. The generic term 'monosaccharide' (as opposed to oligosaccharide or polysaccharide) denotes a single unit, without glycosidic connection to other such units. It includes aldoses, dialdoses, aldoketoses, ketoses and diketoses, as well as deoxy sugars, provided that the parent compound has a (potential) carbonyl group.

"}},{"_id":"987353","name":"concentration","format":"","frictionlessType":"number","description":"

The concentration (µM) of monosaccharide measured at each station and depth

","units":{"_id":"","name":"µM"},"type":{"_id":"910593","name":"monosaccharide","description":"

"}}],"related_publications":[{"id":"987356","citation":"Benner, R., & Amon, R. M. W. (2015). The Size-Reactivity Continuum of Major Bioelements in the Ocean. Annual Review of Marine Science, 7(1), 185–205. https://doi.org/10.1146/annurev-marine-010213-135126\r\n","relation_type":["Methods"],"relation_desc":""},{"id":"964821","citation":"Engel, A., & Händel, N. (2011). A novel protocol for determining the concentration and composition of sugars in particulate and in high molecular weight dissolved organic matter (HMW-DOM) in seawater. Marine Chemistry, 127(1–4), 180–191. https://doi.org/10.1016/j.marchem.2011.09.004\r\n","relation_type":["Methods"],"relation_desc":""},{"id":"988193","citation":"Rühmann, B., Schmid, J., & Sieber, V. (2014). Fast carbohydrate analysis via liquid chromatography coupled with ultra violet and electrospray ionization ion trap detection in 96-well format. Journal of Chromatography A, 1350, 44–50. https://doi.org/10.1016/j.chroma.2014.05.014\r\n","relation_type":["Methods"],"relation_desc":""},{"id":"987355","citation":"Vidal-Melgosa, S., Sichert, A., Francis, T. B., Bartosik, D., Niggemann, J., Wichels, A., Willats, W. G. T., Fuchs, B. M., Teeling, H., Becher, D., Schweder, T., Amann, R., & Hehemann, J.-H. (2021). Diatom fucan polysaccharide precipitates carbon during algal blooms. Nature Communications, 12(1). https://doi.org/10.1038/s41467-021-21009-6\r\n","relation_type":["Methods"],"relation_desc":""},{"id":"988192","citation":"Xu, G., Amicucci, M. J., Cheng, Z., Galermo, A. G., & Lebrilla, C. B. (2018). Revisiting monosaccharide analysis – quantitation of a comprehensive set of monosaccharides using dynamic multiple reaction monitoring. The Analyst, 143(1), 200–207. https://doi.org/10.1039/c7an01530e\r\n","relation_type":["Methods"],"relation_desc":""}],"related_datasets":[],"services":[{"grouping":"","items":[{"name":"View Data","description":"An in-browser view of the primary tabular data file","url":"https://www.bco-dmo.org/dataset/987279/data/view","contentType":"text/html","actionType":"view"}]},{"grouping":"Metadata","items":[{"name":"HTML","description":"A simple HTML page of the dataset metadata","url":"https://www.bco-dmo.org/dataset/987279/description","contentType":"text/html","actionType":"view"},{"name":"PDF","description":"A PDF of the HTML dataset metadata","url":"https://www.bco-dmo.org/dataset/987279/Dataset_description.pdf","contentType":"application/pdf","actionType":"download"},{"name":"ISO 19115-2 (NOAA Profile)","description":"","url":"https://www.bco-dmo.org/dataset/987279/iso","contentType":"application/xml","actionType":"download"}]}],"programs":[],"projects":[{"_id":"896822","name":"Substrate structural complexity and abundance control distinct mechanisms of microbially-driven carbon cycling in the ocean","acronym":"Substrate complexity and microbes","description":"$17"}],"archives":[{"id_value":"10.26008/1912/bco-dmo.987279.1","url":"https://doi.org/10.26008/1912/bco-dmo.987279.1","date_assigned":"2026-08-04","version_date":"2025-10-16","archiver":"Woods Hole Oceanographic Institution (WHOI BCO-DMO)","archiver_id":"191"}],"license":{"name":"Creative Commons Attribution 4.0","id":"CC-BY-4.0","uri":"https://creativecommons.org/licenses/by/4.0/","spdxUri":"https://spdx.org/licenses/CC-BY-4.0"},"metrics":{"views":{"count":42},"downloads":{"count":48}}}}]