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            <gco:CharacterString>Cite this dataset as: Lloyd, C., Bligh, M., Arnosti, C., Ghobrial, S. (2025) Monosaccharide Composition of HMWDOM concentrate and POM-derived Carbohydrates from bulk seawater and mesocosm experiments taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-10-16 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/987279 [access date]</gco:CharacterString>
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        <gco:CharacterString>ENEN683 Monosaccharides DOM POM LV Dataset Description:  Methods and Sampling: &amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Sampling&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Glycans were profiled in bulk and high molecular weight dissolved organic matter (HMWDOM) and particulate organic matter (POM) in the deep chlorophyll max (DCM) and bottom water at three stations in the western North Atlantic Ocean.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Sampling and processing of HMWDOM and POM&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Seawater was collected using 30 L Niskin bottles on-board the R/V Endeavor. Niskin bottles were attached to rosettes equipped with conductivity, temperature and depth (CTD) sensors. Physical parameters were recorded using a Seabird 911+ CTD profiler. Data were processed and binned with SBE Data Processing software (v7.26.7). Collected seawater was transferred into acid-washed carboys before processing on-board. HMWDOM was concentrated to ~300 mL by tangential flow filtration (TFF). The TFF system (Sartorius &amp;amp;lt;&amp;amp;gt;) was run with 3 filter cassettes (1 kDa). HMWDOM samples were further concentrated back in the lab by freeze-drying and resuspension in lower volumes of MilliQ-water; for example, 10 mL of HMWDOM was freeze-dried and resuspended in 2.5 to 3mL. These concentrated HMWDOM samples were used for acid hydrolysis and microarray printing. POM samples were taken using in situ pumps onboard R/V Endeavor (EN683). At station 21 ~393 L were filtered in surface waters and 1276 L between 2800 and 3100 m depth. At station 22 ~214 L were sampled at DCM and 931 L at depths between 3321 and 3694 m. At station 23 the in situ pumps were deployed at 110 m (DCM) and 5100 m (depth) and pumped 515 L and 1196 L, respectively.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Sequential extraction of polysaccharides from GF/F filters&amp;amp;nbsp;&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Seven punchouts of 11 m diameter were made from GF/F filters and sequentially extracted using MilliQ-water, 0.3M EDTA and 4M NaOH + 0.1% NaBH4 (Vidal-Melgosa et al., 2021). Subsequently solvents were added to the filter pieces, vortexed and incubated for 2 h at 60°C at 650 rpm in a heat block. Extracts were centrifuged at 6,000 x g for 15 min at 15°C and the supernatant was transferred to a new tube. NaOH extracts were neutralized with 4M HCl.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Quantification of monosaccharides&amp;lt;/strong&amp;gt;&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Polysaccharides of HMWDOM and POM samples were hydrolysed into monosaccharides. Freeze dried and concentrated HMWDOM samples (500 µL) were acid hydrolysed with 500 µL of 2M HCl. Additionally, 10 x 5 mm diameter punchouts from all GF/F filters were directly transferred to ampoules and acid hydrolysed in 750 µL 1M HCl. All hydrolyses were run at 100°C for 24 hrs. The supernatant of acid hydrolysed samples was dried in an acid-resistant vacuum concentrator (Martin Christ Gefriertrocknungsanlagen GmbH, Germany). Hydrolysates were reconstituted in MilliQ-water and pH adjusted to &amp;amp;gt;7 with 0.1 M NaOH. Monosaccharide standards and hydrolysates were spiked with 13C-labelled glucose, galactose and mannose before derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) (Rühmann et al., 2014). PMP-derivatives were separated on a Agilent 1290 Infinity II LC system equipped with a Waters CORTECS UPLC C18 column and measured on a SCIEX qTRAP5500 by multiple reaction monitoring (MRM) (Xu et al., 2018). Signal intensities were normalised to 13-labelled standards and calibrated against standard curves.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/896821.rdf" xlink:title="OCE-2022952" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2022952 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2022952</gmx:Anchor>
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http://lod.bco-dmo.org/id/dataset-parameter/987340.rdf
	Name: date
	Units: unitless
	Description: &lt;p&gt;Date of sample collection&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/987341.rdf
	Name: time
	Units: unitless
	Description: &lt;p&gt;Time of sample collection, Eastern Time (ET)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/987342.rdf
	Name: ISO_DateTime_UTC
	Units: unitless
	Description: &lt;p&gt;Datetime of collection in UTC&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/987344.rdf
	Name: depth_description
	Units: unitless
	Description: &lt;p&gt;Sequence of depths sampled (d1 is surface; higher numbers at greater depths)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/987345.rdf
	Name: depth_actual
	Units: meters
	Description: &lt;p&gt;Actual depth at which water was collected&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/987347.rdf
	Name: sample_type
	Units: unitless
	Description: &lt;p&gt;HMWDOM = high molecular weight dissolved organic matter or POM = particulate organic matter&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/987348.rdf
	Name: amendment_type
	Units: unitless
	Description: &lt;p&gt;Mesocosm was unamended (U), or amended with high molecular weight organic matter; F, A, T refer to type of organic matter added (Fucoidan, Arabinogalactan, Thalassiosira extract)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/987349.rdf
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	Units: unitless
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http://lod.bco-dmo.org/id/dataset-parameter/987350.rdf
	Name: volume_filtered
	Units: Liters
	Description: &lt;p&gt;The amount of seawater filtered (liters) for particulate organic carbon and monosaccharide composition analysis.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/987351.rdf
	Name: concentrated_Volume
	Units: mL
	Description: &lt;p&gt;Final concentrated volume of sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/987352.rdf
	Name: monosaccharide
	Units: unitless
	Description: &lt;p&gt;The monosaccharide measured&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/987353.rdf
	Name: concentration
	Units: µM
	Description: &lt;p&gt;The concentration (µM) of monosaccharide measured at each station and depth&lt;/p&gt; 
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&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Sequential extraction of polysaccharides from GF/F filters&amp;amp;nbsp;&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Seven punchouts of 11 m diameter were made from GF/F filters and sequentially extracted using MilliQ-water, 0.3M EDTA and 4M NaOH + 0.1% NaBH4 (Vidal-Melgosa et al., 2021). Subsequently solvents were added to the filter pieces, vortexed and incubated for 2 h at 60°C at 650 rpm in a heat block. Extracts were centrifuged at 6,000 x g for 15 min at 15°C and the supernatant was transferred to a new tube. NaOH extracts were neutralized with 4M HCl.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Quantification of monosaccharides&amp;lt;/strong&amp;gt;&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Polysaccharides of HMWDOM and POM samples were hydrolysed into monosaccharides. Freeze dried and concentrated HMWDOM samples (500 µL) were acid hydrolysed with 500 µL of 2M HCl. Additionally, 10 x 5 mm diameter punchouts from all GF/F filters were directly transferred to ampoules and acid hydrolysed in 750 µL 1M HCl. All hydrolyses were run at 100°C for 24 hrs. The supernatant of acid hydrolysed samples was dried in an acid-resistant vacuum concentrator (Martin Christ Gefriertrocknungsanlagen GmbH, Germany). Hydrolysates were reconstituted in MilliQ-water and pH adjusted to &amp;amp;gt;7 with 0.1 M NaOH. Monosaccharide standards and hydrolysates were spiked with 13C-labelled glucose, galactose and mannose before derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) (Rühmann et al., 2014). PMP-derivatives were separated on a Agilent 1290 Infinity II LC system equipped with a Waters CORTECS UPLC C18 column and measured on a SCIEX qTRAP5500 by multiple reaction monitoring (MRM) (Xu et al., 2018). Signal intensities were normalised to 13-labelled standards and calibrated against standard curves.&amp;lt;/p&amp;gt;</gco:CharacterString>
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This category includes vacuum centrifuge concentrator, which include a vacuum chamber within which a centrifuge rotor is mounted for spinning a plurality of vials containing a solution at high speed while subjecting the solution to a vacuum condition for concentration and evaporation. Alternative names: sample concentrator; speed vacuum; speed vac.</gco:CharacterString>
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         <gmx:Anchor xlink:href="http://vocab.nerc.ac.uk/collection/C17/current/32EV" xlink:actuate="onRequest">Community Standard Description</gmx:Anchor>
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              <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/authority/1.rdf" xlink:actuate="onRequest">International Council for the Exploration of the Sea</gmx:Anchor>
            </gmd:title>
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          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/53992.rdf"
           xlink:title="32EV" xlink:actuate="onRequest">R/V Endeavor</gmx:Anchor>
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      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/53992.rdf" xlink:title="R/V Endeavor" xlink:actuate="onRequest">vessel</gmx:Anchor>
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                      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/956118.rdf" xlink:title="Cruise" xlink:actuate="onRequest">EN683</gmx:Anchor>
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          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/53992.rdf"
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      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/53992.rdf" xlink:title="R/V Endeavor" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
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