http://lod.bco-dmo.org/id/dataset/988179
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2025-11-03
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Detection and Quantification of Cells Exhibiting 'Selfish' Uptake in samples taken during R/V Endeavor cruise EN638 in the Western North Atlantic in May 2019
2025-11-03
publication
2025-11-03
revision
BCO-DMO Linked Data URI
2025-11-03
creation
http://lod.bco-dmo.org/id/dataset/988179
Carol Arnosti
University of North Carolina at Chapel Hill
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lloyd, C., Brown, S. A., Giljan, G., Arnosti, C., Ghobrial, S. (2025) Detection and Quantification of Cells Exhibiting 'Selfish' Uptake in samples taken during R/V Endeavor cruise EN638 in the Western North Atlantic in May 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-11-03 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/988179 [access date]
EN638 cell count selfish Dataset Description: Methods and Sampling: <p>Water was collected via Niskin bottles mounted on a rosette, equipped with a CTD, or from mesocosm (large volume) incubations.</p>
<p>For mesocosm (large volume) incubation experiments (referred to as “LV” incubations), seawater was transferred to 20 L carboys that were rinsed three times with water from the sampling depth and then filled with seawater from a single Niskin bottle, using silicone tubing that had been acid washed then rinsed with distilled water prior to use. Four carboys were filled at each depth from bottom water, water from the depth at which oxygen showed a minimum, and deep chlorophyll maximum (DCM) water, according to the CTD. Triplicate 20L carboys were amended with ca. 500 mg (exact mass was recorded for each addition) of HMW <em>Thalassiosira</em>; unamended single carboys were used for controls.&nbsp;All mesocosms were incubated in the dark at near in-situ temperatures. Mesocosms were sub-sampled at the start of incubation (0 days), and then after at approximately 3 d, 5 or 7 d, 10 d, 15 d, and 30 d for multiple assays including: bacterial production using 3H-Leucine, dissolved organic carbon (DOC), nutrients, bacterial cell counts, peptidase and glucosidase activity measurements in addition to&nbsp;the potential of the seawater microbial community to hydrolyze six high-molecular-weight polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan). Bacterial cell counts presented here are from unamended incubations</p>
<p>For each depth and mesocosm sample, 20-30 ml of 1% formaldehyde (FA) fixed sample were filtered through a 0.2 µm pore size poly-carbonate filter, applying a maximum vacuum of 200 mbar. Nucleic acids of filtered cells were counterstained with 4',6-diamidino-2-phenylindole (DAPI) and mounted using a Citifluor/VectaShield (4:1) solution. A fully automated epifluorescence microscope (Zeiss AxioImager.Z2 microscope stand, Carl Zeiss Jena, Germany) equipped with a cooled charged-coupled-device (CCD) camera (AxioCam MRm + Colibri LED light source, Carl Zeiss), a light-emitting diode for DAPI (UV-emitting LED, 365 nm) and a HE-62&nbsp;multi filter module with a triple emission filter (425/50 nm, 527/54 nm, LP 615 nm, including a triple beam splitter of 395/495/610, Carl Zeiss). As described by Bennke <em>et al.,</em> 2016, a minimum of 45 fields of view (FOV) per sample were acquired using a 63x magnification oil immersion plan apochromatic objective with a numerical aperture of 1.4 (Carl Zeiss). Cell counting was performed with the image analysis software ACMETOOL (Zeder, M. 2005-2021, Software for Biology,&nbsp;http://www.technobiology.ch and Max Planck Institute for marine microbiology, Bremen). Validation of the automated counts&nbsp;was done by manual cell counting.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1736772 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1736772
onGoing
Carol Arnosti
University of North Carolina at Chapel Hill
919-962-5754
Dept. of Marine Sciences 3117A Venable/Murray Hall
Chapel Hill
NC
27599-3300
USA
arnosti@email.unc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
deployment
station
latitude
longitude
ISO_DateTime_UTC
cast_number
depth_name
depth_actual
sample_type
unamended_amended
substrate
timepoint_days
timepoint_hours
replicate_1_selfish_percent
replicate_2_selfish_percent
replicate_3_selfish_percent
average_selfish_percent
standard_deviation_selfish_percent
replicate_1_substratecells
replicate_2_substratecells
replicate_3_substratecells
average_substratecells
standard_deviation_substratecells
replicate_1_cells
replicate_2_cells
replicate_3_cells
average_cells
standard_deviation_cells
Zeiss LSM780 with Airyscan (Carl Zeiss)
CTD
Zeiss AxioImager.Z2 microscope stand, Carl Zeiss
Niskin bottles
theme
None, User defined
Cruise identfier
station
latitude
longitude
ISO_DateTime_UTC
cast
depth_comment
depth
sample type
treatment
time_point
abundance
mean
standard deviation
featureType
BCO-DMO Standard Parameters
Confocal Laser Scanning Microscope
CTD Sea-Bird SBE 911plus
Fluorescence Microscope
Niskin bottle
instrument
BCO-DMO Standard Instruments
EN638
service
Deployment Activity
Northern Atlantic
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
A mechanistic microbial underpinning for the size-reactivity continuum of dissolved organic carbon degradation
https://osprey.bco-dmo.org/project/809975
A mechanistic microbial underpinning for the size-reactivity continuum of dissolved organic carbon degradation
<p><em>NSF Award Abstract:</em><br />
Marine dissolved organic matter (DOM) is one of the largest actively-cycling reservoirs of organic carbon on the planet, and thus a major component of the global carbon cycle. The high molecular weight (HMW) fraction of DOM is younger in age and more readily consumed by microbes than lower molecular weight (LMW) fractions of DOM, but the reasons for this difference in reactivity between HMW DOM and LMW DOM are unknown. Two factors may account for the greater reactivity of HMW DOM: (i) targeted uptake of HMW DOM by specific bacteria, a process the PI and her collaborators at the Max Planck Institute for Marine Microbiology (MPI) recently identified in surface ocean waters; and (ii) a greater tendency of HMW DOM to aggregate and form gels and particles, which can be colonized by bacteria that are well-equipped to breakdown organic matter. Scientists and students from the University of North Carolina (UNC) - Chapel Hill will collaborate with researchers at the MPI for Marine Microbiology (Bremen, Germany) to investigate this breakdown of HMW DOM by marine microbial communities. These investigations will include a field expedition in the North Atlantic, during which HMW DOM degradation rates and patterns will be compared in different water masses and under differing conditions of organic matter availability. DOM aggregation potential, and degradation rates of these aggregates, will also be assessed. Specialized microscopy will be used in order to pinpoint HMW DOM uptake mechanisms and rates. The work will be complemented by ongoing studies of specific bacteria that breakdown HMW DOM, their genes, and their proteins. Graduate as well as undergraduate students will participate as integral members of the research team in all aspects of the laboratory and field work; aspects of the project will also be integrated into classes the scientist teaches at UNC.</p>
<p>The existence of a size-reactivity continuum of DOM - observations and measurements showing that HMW DOM tends to be younger and more reactive than lower MW DOM - has been demonstrated in laboratory and field investigations in different parts of the ocean. A mechanistic explanation for the greater reactivity of HMW DOM has been lacking, however. This project will investigate the mechanisms and measure rates of HMW DOM degradation, focusing on identifying the actors and determining the factors that contribute to rapid cycling of HMW DOM. Collaborative work at UNC and MPI-Bremen recently identified a new mechanism of HMW substrate uptake common among pelagic marine bacteria: these bacteria rapidly bind, partially hydrolyze, and transport directly across the outer membrane large fragments of HMW substrates that can then be degraded within the periplasmic space, avoiding production of LMW DOM in the external environment. This mode of substrate processing has been termed selfish, since targeted HMW substrate uptake sequesters resources away from other members of microbial communities. Measurements and models thus must account for three modes of substrate utilization in the ocean: selfish, sharing (external hydrolysis, leading to low molecular weight products), and scavenging (uptake of low molecular weight hydrolysis products without production of extracellular enzymes). Using field studies as well as mesocosm experiments, the research team will investigate the circumstances and locations at which different modes of substrate uptake predominate. A second focal point of the project is to determine the aggregation potential and microbial degradation of aggregated HMW DOM. Preliminary studies have demonstrated that particle-associated microbial communities utilize a broader range of enzymatic capabilities than their free-living counterparts. These capabilities equip particle-associated communities to effectively target a broad range of complex substrates. The project will thus focus on two key aspects of HMW DOM - the abilities of specialized bacteria to selectively sequester HMW substrates, as well as the greater potential of HMW substrates to aggregate ? and will quantify these factors at different locations and depths in the ocean. The project will thereby provide a mechanistic underpinning for observations of the DOC size-reactivity continuum, an essential part of developing an overall mechanistic understanding of organic matter degradation in the ocean.</p>
Microbial DOC Degradation
largerWorkCitation
project
eng; USA
oceans
Northern Atlantic
-72.0021
-53.3949
34.6369
42.83954
2019-05-17
2019-05-25
Northern Atlantic, Southern Indian Ocean, Svalbard
0
BCO-DMO catalogue of parameters from Detection and Quantification of Cells Exhibiting 'Selfish' Uptake in samples taken during R/V Endeavor cruise EN638 in the Western North Atlantic in May 2019
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/988364.rdf
Name: deployment
Units: unitless
Description: <p>Cruise ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/988365.rdf
Name: station
Units: unitless
Description: <p>Station number for cruise (18, 19, or 20)</p>
http://lod.bco-dmo.org/id/dataset-parameter/988366.rdf
Name: latitude
Units: decimal degrees
Description: <p>Latitude, south is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/988368.rdf
Name: longitude
Units: decimal degrees
Description: <p>Longitude, west is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/988370.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time of sample collection in ISO format, US Eastern Time (UTC-05:00)</p>
http://lod.bco-dmo.org/id/dataset-parameter/988372.rdf
Name: cast_number
Units: unitless
Description: <p>Cast number (refers to cast of CTD/Niskin bottles on cruise)</p>
http://lod.bco-dmo.org/id/dataset-parameter/988373.rdf
Name: depth_name
Units: unitless
Description: <p>Water column feature or oceanic zone sampled (Surface, DCM, 300m, or bottom/near bottom)</p>
http://lod.bco-dmo.org/id/dataset-parameter/988374.rdf
Name: depth_actual
Units: meters (m)
Description: <p>Actual depth at which water was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/988376.rdf
Name: sample_type
Units: unitless
Description: <p>Sample from bulk water or Large Volume incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/988377.rdf
Name: unamended_amended
Units: unitless
Description: <p>Whether high molecular weight organic matter was added or not; U for unamended</p>
http://lod.bco-dmo.org/id/dataset-parameter/988378.rdf
Name: substrate
Units: unitless
Description: <p>Polysaccharide used for incubation: ara = arabinogalactan, chn = chondroitin sulfate, fuc = fucoidan, lam = laminarin, pul = pullulan, xyl = xylan, or control (no substrate added)</p>
http://lod.bco-dmo.org/id/dataset-parameter/988379.rdf
Name: timepoint_days
Units: days
Description: <p>Days post amendment when subsample was taken for substrate addition and enzymatic activity measurement</p>
http://lod.bco-dmo.org/id/dataset-parameter/988380.rdf
Name: timepoint_hours
Units: hours
Description: <p>Hours post amendment when subsample was taken for substrate addition and enzymatic activity measurement</p>
http://lod.bco-dmo.org/id/dataset-parameter/988381.rdf
Name: replicate_1_selfish_percent
Units: percent
Description: <p>Replicate sample 1 of selfish percent. Blank value indicates sample not available for counting or autofluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/988382.rdf
Name: replicate_2_selfish_percent
Units: percent
Description: <p>Replicate sample 2 of selfish percent. Blank value indicates sample not available for counting or autofluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/988383.rdf
Name: replicate_3_selfish_percent
Units: percent
Description: <p>Replicate sample 3 of selfish percent. Blank value indicates sample not available for counting or autofluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/988384.rdf
Name: average_selfish_percent
Units: percent
Description: <p>Average selfish percent of the three replicates. Blank value indicates sample not available for counting or autofluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/988385.rdf
Name: standard_deviation_selfish_percent
Units: percent
Description: <p>Standard deviation of the average selfish percent of the three replicates. Blank value indicates sample not available for counting or autofluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/988386.rdf
Name: replicate_1_substratecells
Units: Cells/ml
Description: <p>Replicate sample 1 of number of substrate cells per ml. Blank value indicates sample not available for counting or autofluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/988387.rdf
Name: replicate_2_substratecells
Units: Cells/ml
Description: <p>Replicate sample 2 of number of substrate cells per ml. Blank value indicates sample not available for counting or autofluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/988388.rdf
Name: replicate_3_substratecells
Units: Cells/ml
Description: <p>Replicate sample 3 of number of substrate cells per ml. Blank value indicates sample not available for counting or autofluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/988389.rdf
Name: average_substratecells
Units: Cells/ml
Description: <p>Average number of substrate cells per ml of the three replicates. Blank value indicates sample not available for counting or autofluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/988390.rdf
Name: standard_deviation_substratecells
Units: Cells/ml
Description: <p>Standard deviation of the average number of substrate cells per ml of the three replicates. Blank value indicates sample not available for counting or autofluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/988391.rdf
Name: replicate_1_cells
Units: Cells/ml
Description: <p>Replicate sample 1 of number of cells per ml. Blank value indicates sample not available for counting or autofluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/988392.rdf
Name: replicate_2_cells
Units: Cells/ml
Description: <p>Replicate sample 2 of number of cells per ml. Blank value indicates sample not available for counting or autofluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/988393.rdf
Name: replicate_3_cells
Units: Cells/ml
Description: <p>Replicate sample 3 of number cells per ml. Blank value indicates sample not available for counting or autofluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/988394.rdf
Name: average_cells
Units: Cells/ml
Description: <p>Average number of cells per ml of the three replicates. Blank value indicates sample not available for counting or autofluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/988395.rdf
Name: standard_deviation_cells
Units: Cells/ml
Description: <p>Standard deviation of the average number of cells per ml of the three replicates. Blank value indicates sample not available for counting or autofluorescence</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/988179/data/download
download
onLine
dataset
<p>Water was collected via Niskin bottles mounted on a rosette, equipped with a CTD, or from mesocosm (large volume) incubations.</p>
<p>For mesocosm (large volume) incubation experiments (referred to as “LV” incubations), seawater was transferred to 20 L carboys that were rinsed three times with water from the sampling depth and then filled with seawater from a single Niskin bottle, using silicone tubing that had been acid washed then rinsed with distilled water prior to use. Four carboys were filled at each depth from bottom water, water from the depth at which oxygen showed a minimum, and deep chlorophyll maximum (DCM) water, according to the CTD. Triplicate 20L carboys were amended with ca. 500 mg (exact mass was recorded for each addition) of HMW <em>Thalassiosira</em>; unamended single carboys were used for controls.&nbsp;All mesocosms were incubated in the dark at near in-situ temperatures. Mesocosms were sub-sampled at the start of incubation (0 days), and then after at approximately 3 d, 5 or 7 d, 10 d, 15 d, and 30 d for multiple assays including: bacterial production using 3H-Leucine, dissolved organic carbon (DOC), nutrients, bacterial cell counts, peptidase and glucosidase activity measurements in addition to&nbsp;the potential of the seawater microbial community to hydrolyze six high-molecular-weight polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan). Bacterial cell counts presented here are from unamended incubations</p>
<p>For each depth and mesocosm sample, 20-30 ml of 1% formaldehyde (FA) fixed sample were filtered through a 0.2 µm pore size poly-carbonate filter, applying a maximum vacuum of 200 mbar. Nucleic acids of filtered cells were counterstained with 4',6-diamidino-2-phenylindole (DAPI) and mounted using a Citifluor/VectaShield (4:1) solution. A fully automated epifluorescence microscope (Zeiss AxioImager.Z2 microscope stand, Carl Zeiss Jena, Germany) equipped with a cooled charged-coupled-device (CCD) camera (AxioCam MRm + Colibri LED light source, Carl Zeiss), a light-emitting diode for DAPI (UV-emitting LED, 365 nm) and a HE-62&nbsp;multi filter module with a triple emission filter (425/50 nm, 527/54 nm, LP 615 nm, including a triple beam splitter of 395/495/610, Carl Zeiss). As described by Bennke <em>et al.,</em> 2016, a minimum of 45 fields of view (FOV) per sample were acquired using a 63x magnification oil immersion plan apochromatic objective with a numerical aperture of 1.4 (Carl Zeiss). Cell counting was performed with the image analysis software ACMETOOL (Zeder, M. 2005-2021, Software for Biology,&nbsp;http://www.technobiology.ch and Max Planck Institute for marine microbiology, Bremen). Validation of the automated counts&nbsp;was done by manual cell counting.</p>
Specified by the Principal Investigator(s)
<p>Image analysis was performed with ACMETOOL (Zeder, M. 2005-2021, Software for Biology,&nbsp;http://www.technobiology.ch and Max Planck Institute for marine microbiology, Bremen, version 3) and Zen software package (Carl Zeiss).</p>
Specified by the Principal Investigator(s)
- Opened "20250811_EN638_cellcount_selfish_BCODMO.csv" in Excel and removed all the replicate measurements, leaving only the sample information
- Removed the duplicate rows in this file to produce a unique list of all sample instances
- Saved file as "unique_sample_only.csv" and imported into the BCO-DMO system
- Imported "20250811_EN638_cellcount_selfish_BCODMO.csv" into the BCO-DMO system
- Duplicated "20250811_EN638_cellcount_selfish_BCODMO.csv" three times and filtered by "variable" creating three duplicates, each containing only one variable
- Joined these filtered datasets to the "unique_sample_only.csv" dataset
- Exported file as "988179_v1_en638_cellcount_selfish.csv"
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Zeiss LSM780 with Airyscan (Carl Zeiss)
Zeiss LSM780 with Airyscan (Carl Zeiss)
PI Supplied Instrument Name: Zeiss LSM780 with Airyscan (Carl Zeiss) PI Supplied Instrument Description:Zeiss LSM780 with Airyscan (Carl Zeiss) using a 405 nm, a 488 nm, and a 561 nm laser with detection windows of 420–480 nm, 500–550 nm, and LP 605 nm, respectively. Instrument Name: Confocal Laser Scanning Microscope Instrument Short Name: Instrument Description: A laser scanning confocal microscope is a type of confocal microscope that obtains high-resolution optical images with depth selectivity, in which a laser beam passes through a light source aperture and then is focused by an objective lens into a small (ideally diffraction-limited) focal volume within or on the surface of a specimen.
The confocal microscope uses fluorescence optics. 'Confocal' means that the image is obtained from the focal plane only, any noise resulting from sample thickness being removed optically. 'Laser scanning' means the images are acquired point by point under localized laser excitation rather than full sample illumination, as in conventional widefield microscopy.
CTD
CTD
PI Supplied Instrument Name: CTD PI Supplied Instrument Description:Water was collected via Niskin bottles mounted on a rosette, equipped with a CTD, or from mesocosm (large volume) incubations. Instrument Name: CTD Sea-Bird SBE 911plus Instrument Short Name:CTD SBE 911plus Instrument Description: The Sea-Bird SBE 911 plus is a type of CTD instrument package for continuous measurement of conductivity, temperature and pressure. The SBE 911 plus includes the SBE 9plus Underwater Unit and the SBE 11plus Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 plus and SBE 11 plus is called a SBE 911 plus. The SBE 9 plus uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 plus and SBE 4). The SBE 9 plus CTD can be configured with up to eight auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0058/
Zeiss AxioImager.Z2 microscope stand, Carl Zeiss
Zeiss AxioImager.Z2 microscope stand, Carl Zeiss
PI Supplied Instrument Name: Zeiss AxioImager.Z2 microscope stand, Carl Zeiss PI Supplied Instrument Description:Zeiss AxioImager.Z2 microscope stand, Carl Zeiss - Fully automated epifluorescence microscope Instrument Name: Fluorescence Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/
Niskin bottles
Niskin bottles
PI Supplied Instrument Name: Niskin bottles PI Supplied Instrument Description:Water was collected via Niskin bottles mounted on a rosette, equipped with a CTD, or from mesocosm (large volume) incubations. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: EN638
EN638
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
EN638
Carol Arnosti
University of North Carolina at Chapel Hill
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel