http://lod.bco-dmo.org/id/dataset/988600 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2025-11-10 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) EPR 9-10 North Electron Microprobe Microprobe Elemental Maps from the Hot2cold Vents project 2025-11-10 publication 2025-11-10 revision BCO-DMO Linked Data URI 2025-11-10 creation http://lod.bco-dmo.org/id/dataset/988600 Brandy Marie Toner University of Minnesota Twin Cities principalInvestigator Rose Jones University of Minnesota principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Jones, R., Toner, B. M. (2025) EPR 9-10 North Electron Microprobe Microprobe Elemental Maps. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-11-10 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/988600 [access date] Methods and Sampling: &lt;p&gt;Seafloor sulfide mineral samples were collected from hydrothermally active chimneys and inactive off-axis massive sulfide deposits at East Pacific Rise (EPR) 9.50°N during 2019–2021 on cruises AT42-09, AT42-21, and RR2102. Samples were collected using the HOV &lt;em&gt;Alvin&lt;/em&gt; or ROV &lt;em&gt;Jason II&lt;/em&gt;. Samples were placed in a positive-pressure glove bag flushed with N₂(g), sparged with N₂, heat-sealed into Mylar bags containing AnaeroPaks, and stored at −20 °C.&lt;/p&gt; &lt;p&gt;Epoxy-embedded petrographic thin sections were prepared from these samples. Subsamples were dried for 24 hours under N₂(g), vacuum-embedded in epoxy resin (Struers EpoFix Resin, 1 L kit; Part #40200030) following the manufacturer’s guidelines, and cured under N₂(g) for 24 hours. Each epoxy-embedded sample was pre-cut using a wafering saw and then sent to Spectrum Petrographics, Inc. (Washington, USA) for preparation as 30 µm thick, double-polished thin sections mounted on quartz slides, using methods designed to limit sample exposure to water and ambient air. When not in use, thin sections were stored under N₂(g) to limit oxidation.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756339 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756339 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756558 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756558 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756419 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756419 onGoing Brandy Marie Toner University of Minnesota Twin Cities 612-624-1362 450 Borlaug Hall 1991 Upper Buford Circle St Paul MN 55108-6028 USA toner@umn.edu pointOfContact Rose Jones University of Minnesota rosejones233@gmail.com pointOfContact asNeeded Dataset Version: 1 Unknown JEOL JXA-8530FPlus Electron Probe Microanalyzer theme None, User defined Electron Microprobe instrument BCO-DMO Standard Instruments AT42-09 AT42-21 RR2102 service Deployment Activity otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: From hot to cold in the dark - shifts in seafloor massive sulfide microbial communities as physical and geochemical conditions change after venting ceases https://osprey.bco-dmo.org/project/784386 Collaborative Research: From hot to cold in the dark - shifts in seafloor massive sulfide microbial communities as physical and geochemical conditions change after venting ceases <p><em>NSF Award Abstract:</em><br /> Hydrothermal vents, which deposit seafloor massive sulfides (SMS), occur along the 89,000 km of mid-ocean ridges, submarine volcanoes, and backarc basins that occur at tectonic plate boundaries in the ocean. Active hydrothermal vent sulfide chimneys are hotspots of biodiversity and productivity in the deep ocean, as well as potential resources for metals. While significant effort has focused on understanding the diversity of biological communities and geochemistry associated with actively venting SMS, relatively little is known about the biological communities associated with SMS once venting ceases. Furthermore, little is known about the microbiological and geochemical changes that occur during the transition period from active to inactive, during which an important succession occurs in the microbial community and geochemistry of fluids within the chimney. This interdisciplinary project will create and sample this transition period by collecting multiple active SMS samples from individual vents at 9 degrees N East Pacific Rise and allowing them to transition to inactive on the seafloor, mimicking the end of venting while allowing for the exact time when venting ceased to be known, something not possible when sampling naturally formed inactive SMS. Microbial community diversity and metabolism will be analyzed in parallel with bulk and fine-scale geological measurements for active, transitioning, and inactive sulfides. This seafloor experimental and analytical approach will provide knowledge of how microbial communities, rates of biogeochemical transformations, and geological conditions change as SMS transition from hot and actively venting to cold and inactive. Students in grades 6-8 will be entrained into the project through research cruise "ship-to-shore" interactions and communications, post-cruise workshops for educators working with students typically underrepresented in STEM fields, and a collaboration with the Science, Engineering, Art and Design Gallery (SEAD), a community and economic development project in Bryan, TX.</p> <p>Hydrothermal vents are quantitatively important to the biology and chemistry of the deep ocean, but the vast majority of current knowledge focuses on actively venting deposits. However, after venting ceases, sulfides can persist on the seafloor for tens of thousands of years, making them long-lived, globally-abundant microbial substrates. In recent years, studies of inactive SMS found drastically different microbial communities than those on active deposits, indicating a succession of the microbial community, and thus a potentially different impact on deep ocean biodiversity and biogeochemistry than actively venting deposits. However, ages of the inactive structures are often not known, so it is impossible to estimate how quickly these changes occur, and how quickly co-occurring changes in sulfide mineralogy and microbiological communities occur. This project will provide the first insight into what happens at the microbial and mineralogical level as SMS initially transition from active to inactive. Active SMS will be sampled and analyzed for microbial community composition, functional capacity, gene expression and metabolic rates. Co-located subsamples will be analyzed for porosity and bulk and fine-scale mineralogy. Subsamples of those active SMS samples will be left on the seafloor to incubate and be collected weeks and a year or more later, with the same analyses conducted upon collection. This will allow for determination of microbiological and mineralogical changes that occur during that initial transition and for comparison with older inactive SMS from the same vent fields. Together, the data collected will be integrated to generate a conceptual model of succession of biology, mineralogy, porosity and pore distribution as vent deposits transition from active to inactive. This project will fill a knowledge gap about hydrothermal ecosystems and has the potential to transform the current understanding of diversity and rates of change in these important seafloor biomes.</p> Hot2cold Vents largerWorkCitation project eng; USA oceans -104.2935759 -104.1749216 9.495434 9.89478369 2019-03-25 2021-04-25 East Pacific Rise 0 BCO-DMO catalogue of parameters from EPR 9-10 North Electron Microprobe Microprobe Elemental Maps from the Hot2cold Vents project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact https://osprey.bco-dmo.org/dataset/988600/data/download download onLine dataset &lt;p&gt;Seafloor sulfide mineral samples were collected from hydrothermally active chimneys and inactive off-axis massive sulfide deposits at East Pacific Rise (EPR) 9.50°N during 2019–2021 on cruises AT42-09, AT42-21, and RR2102. Samples were collected using the HOV &lt;em&gt;Alvin&lt;/em&gt; or ROV &lt;em&gt;Jason II&lt;/em&gt;. Samples were placed in a positive-pressure glove bag flushed with N₂(g), sparged with N₂, heat-sealed into Mylar bags containing AnaeroPaks, and stored at −20 °C.&lt;/p&gt; &lt;p&gt;Epoxy-embedded petrographic thin sections were prepared from these samples. Subsamples were dried for 24 hours under N₂(g), vacuum-embedded in epoxy resin (Struers EpoFix Resin, 1 L kit; Part #40200030) following the manufacturer’s guidelines, and cured under N₂(g) for 24 hours. Each epoxy-embedded sample was pre-cut using a wafering saw and then sent to Spectrum Petrographics, Inc. (Washington, USA) for preparation as 30 µm thick, double-polished thin sections mounted on quartz slides, using methods designed to limit sample exposure to water and ambient air. When not in use, thin sections were stored under N₂(g) to limit oxidation.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;Data was analyzed using probe software (https://www.probesoftware.com/).&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact JEOL JXA-8530FPlus Electron Probe Microanalyzer JEOL JXA-8530FPlus Electron Probe Microanalyzer PI Supplied Instrument Name: JEOL JXA-8530FPlus Electron Probe Microanalyzer PI Supplied Instrument Description:Thin sections were analyzed for elemental distributon at the Electron Microprobe Laboratory, Earth and Environmental Sciences Univeristy of Minnesota-Twin Cities, MN. elemental map measurements were collected from carbon-coated thin sections using a JEOL JXA-8530FPlus Electron Probe Microanalyzer. Instrument Name: Electron Microprobe Instrument Short Name: Instrument Description: Instruments that chemically analyse a small area of a sample by bombarding it with electrons and spectroscopically assaying the resulting X-Ray emissions. Cruise: AT42-09 AT42-09 R/V Atlantis Community Standard Description International Council for the Exploration of the Sea R/V Atlantis vessel AT42-09 Jason B. Sylvan Texas A&M University Cruise: AT42-21 AT42-21 R/V Atlantis Community Standard Description International Council for the Exploration of the Sea R/V Atlantis vessel AT42-21 Jason B. Sylvan Texas A&M University Cruise: RR2102 RR2102 R/V Roger Revelle Community Standard Description International Council for the Exploration of the Sea R/V Roger Revelle vessel RR2102 Daniel Fornari Woods Hole Oceanographic Institution R/V Atlantis Community Standard Description International Council for the Exploration of the Sea R/V Atlantis vessel R/V Roger Revelle Community Standard Description International Council for the Exploration of the Sea R/V Roger Revelle vessel