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                            <gco:CharacterString>&lt;p&gt;&lt;em&gt;NSF Award Abstract:&lt;/em&gt;&lt;br /&gt;
Hydrothermal vents, which deposit seafloor massive sulfides (SMS), occur along the 89,000 km of mid-ocean ridges, submarine volcanoes, and backarc basins that occur at tectonic plate boundaries in the ocean. Active hydrothermal vent sulfide chimneys are hotspots of biodiversity and productivity in the deep ocean, as well as potential resources for metals. While significant effort has focused on understanding the diversity of biological communities and geochemistry associated with actively venting SMS, relatively little is known about the biological communities associated with SMS once venting ceases. Furthermore, little is known about the microbiological and geochemical changes that occur during the transition period from active to inactive, during which an important succession occurs in the microbial community and geochemistry of fluids within the chimney. This interdisciplinary project will create and sample this transition period by collecting multiple active SMS samples from individual vents at 9 degrees N East Pacific Rise and allowing them to transition to inactive on the seafloor, mimicking the end of venting while allowing for the exact time when venting ceased to be known, something not possible when sampling naturally formed inactive SMS. Microbial community diversity and metabolism will be analyzed in parallel with bulk and fine-scale geological measurements for active, transitioning, and inactive sulfides. This seafloor experimental and analytical approach will provide knowledge of how microbial communities, rates of biogeochemical transformations, and geological conditions change as SMS transition from hot and actively venting to cold and inactive. Students in grades 6-8 will be entrained into the project through research cruise &quot;ship-to-shore&quot; interactions and communications, post-cruise workshops for educators working with students typically underrepresented in STEM fields, and a collaboration with the Science, Engineering, Art and Design Gallery (SEAD), a community and economic development project in Bryan, TX.&lt;/p&gt;
&lt;p&gt;Hydrothermal vents are quantitatively important to the biology and chemistry of the deep ocean, but the vast majority of current knowledge focuses on actively venting deposits. However, after venting ceases, sulfides can persist on the seafloor for tens of thousands of years, making them long-lived, globally-abundant microbial substrates. In recent years, studies of inactive SMS found drastically different microbial communities than those on active deposits, indicating a succession of the microbial community, and thus a potentially different impact on deep ocean biodiversity and biogeochemistry than actively venting deposits. However, ages of the inactive structures are often not known, so it is impossible to estimate how quickly these changes occur, and how quickly co-occurring changes in sulfide mineralogy and microbiological communities occur. This project will provide the first insight into what happens at the microbial and mineralogical level as SMS initially transition from active to inactive. Active SMS will be sampled and analyzed for microbial community composition, functional capacity, gene expression and metabolic rates. Co-located subsamples will be analyzed for porosity and bulk and fine-scale mineralogy. Subsamples of those active SMS samples will be left on the seafloor to incubate and be collected weeks and a year or more later, with the same analyses conducted upon collection. This will allow for determination of microbiological and mineralogical changes that occur during that initial transition and for comparison with older inactive SMS from the same vent fields. Together, the data collected will be integrated to generate a conceptual model of succession of biology, mineralogy, porosity and pore distribution as vent deposits transition from active to inactive. This project will fill a knowledge gap about hydrothermal ecosystems and has the potential to transform the current understanding of diversity and rates of change in these important seafloor biomes.&lt;/p&gt;</gco:CharacterString>
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                <gco:CharacterString>&amp;lt;p&amp;gt;Seafloor sulfide mineral samples were collected from hydrothermally active chimneys and inactive off-axis massive sulfide deposits at East Pacific Rise (EPR) 9.50°N in 2019-2021 on cruises AT42-09, AT42-21 and RR2102. Samples were collected using the HOV Alvin or ROV JASON II. Samples were placed in a positive-pressure glove bag flushed with N2 (g), sparged with N2, heat-sealed into mylar bags containing Anaeropaks, and stored at -20°C. Epoxy embedded petrographic thin sections were created using these samples. Subsamples were dried for 24 hours under N2 (g), vacuum embedded in epoxy resin (Struers EpoFix Resin, 1L kit: Part #40200030) following manufacturer’s guidelines, and cured under N2 (g) for 24 hours. Each epoxy-embedded sample was pre-cut using a wafering saw then sent to Spectrum Petrographics, Inc. (Washington, USA) for preparation as 30 micron thick, double-polished thin sections mounted on quartz slides using methods to limit sample exposure to water and ambient air. When not in use, thin-sections were stored under N2 (g) to limit oxidation.&amp;amp;nbsp;&amp;lt;/p&amp;gt;</gco:CharacterString>
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          </gmd:processStep>
        <gmd:processStep xlink:title="BCO-DMO Data Processing Description">
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                  <gco:CharacterString>- Loaded 16 CSV files (e.g., ALV5014-02-R02b1(C04).csv, ALV5019-03R01aC01(ALV5018-01-R03a).csv, Sample(ALV5013-01-R01B-h).csv, and others) using filenames as table names; headers from row 2; missing values set to &amp;quot;&amp;quot; and &amp;quot;nd&amp;quot;
- Concatenated all 16 tables into a single table named &amp;quot;merged&amp;quot;, mapping the blank first column to sample_ID and adding an original_source_filename column tracking the source file for each row
- Renamed elemental concentration columns (O, F, Al, Si, S, Ca, Fe, Cu, As, Rh, W, Na, Mg, Cl, K, Mn, Ni, Zn, Pd, Os, Ge, Mo, Ir, V, I, Ta, Ag, Cd, Sb, Se, Au) to weight_concentration_percentage_ prefixed names
- Reordered columns placing sample_ID first, followed by weight concentration columns, then Total, Chi Squared, and original_source_filename
- Loaded metadata from EM percent metadata.xlsx (sheet 1) into table &amp;quot;EM_percent_metadata&amp;quot;; missing values set to &amp;quot;&amp;quot; and &amp;quot;nd&amp;quot;
- Split sample_ID in &amp;quot;merged&amp;quot; on delimiter &amp;quot;@&amp;quot; into join_key_base and join_key_suffix; constructed a join_key by appending &amp;quot;].csv&amp;quot; to join_key_base; deleted join_key_base and join_key_suffix
- Joined &amp;quot;EM_percent_metadata&amp;quot; into &amp;quot;merged_copy&amp;quot; using File name (source) matched to join_key (target) in half-outer mode, bringing in Date_collected_MMDDYYYY, Depth_mbs, Heading_deg, Latitude_dd, Longitude_dd, Map name, Sample name, and Time_collected; deleted the EM_percent_metadata table after join
- Renamed sample_ID to replicate_ID; reordered columns placing Sample_name and Map_name first, followed by replicate_ID and original_source_filename, then navigation/temporal fields, weight concentration columns, Total, Chi_Squared
- Converted Date_collected_MMDDYYYY (format %m-%d-%y) combined with Time_collected (format %H:%M:%S) from UTC to ISO 8601 datetime string output field ISO_DateTime_Collected_UTC
- Reordered columns to insert ISO_DateTime_Collected_UTC after Time_collected
- Converted Date_collected_MMDDYYYY alone (format %m-%d-%y) to ISO 8601 datetime output field ISO_DateTime_UTC
- Output for the primary data file written to 988636_v1_EPR_9-10_North_Electron_Microprobe_Seafloor_Sulfide_Bulk_Composition.csv</gco:CharacterString>
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                <gmd:source>
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      <gmd:contact>
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    <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/191.rdf" xlink:actuate="onRequest">Biological and Chemical Oceanography Data Management Office (BCO-DMO)</gmx:Anchor>
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		  <gmd:address>
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				    <gco:CharacterString>USA</gco:CharacterString>
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      <gmd:onlineResource>
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		  <gmd:hoursOfService>
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		  <gmd:contactInstructions>
		    <gco:CharacterString>For questions regarding this resource, please contact BCO-DMO via the email address provided.</gco:CharacterString>
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  <gmd:role>
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    <gmi:MI_AcquisitionInformation>
    <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/996057.rdf" xlink:title="Electron Microprobe" xlink:actuate="onRequest">JEOL JXA-8530FPlus Electron Probe Microanalyzer</gmx:Anchor>
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          <gmi:type>
            <gco:CharacterString>JEOL JXA-8530FPlus Electron Probe Microanalyzer</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: JEOL JXA-8530FPlus Electron Probe Microanalyzer PI Supplied Instrument Description:Bulk elemental composition of thin-sections were analyzed at the Electron Microprobe Laboratory, Earth and Environmental Sciences University of Minnesota-Twin Cities, MN. elemental map measurements were collected from carbon-coated thin sections using a JEOL JXA-8530FPlus Electron Probe Microanalyzer. Instrument Name: Electron Microprobe Instrument Short Name:   Instrument Description: Instruments that chemically analyse a small area of a sample by bombarding it with electrons and spectroscopically assaying the resulting X-Ray emissions.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/880039.rdf" xlink:title="HOV Alvin" xlink:actuate="onRequest">HOV Alvin</gmx:Anchor>
              </gmd:code>
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            <gco:CharacterString>PI Supplied Instrument Name: HOV Alvin PI Supplied Instrument Description:Samples were collected using the HOV Alvin or ROV JASON II on oceanographic expeditions (cruises) AT42-09, AT42-21, and RR2102. Instrument Name: HOV Alvin Instrument Short Name:   Instrument Description: Human Occupied Vehicle (HOV) Alvin is part of the National Deep Submergence Facility (NDSF). Alvin enables in-situ data collection and observation by two scientists to depths reaching 6,500 meters, during dives lasting up to ten hours.

Commissioned in 1964 as one of the world’s first deep-ocean submersibles, Alvin has remained state-of-the-art as a result of numerous overhauls and upgrades made over its lifetime. The most recent upgrades, begun in 2011 and completed in 2021, saw the installation of a new, larger personnel sphere with a more ergonomic interior; improved visibility and overlapping fields of view; longer bottoms times; new lighting and high-definition imaging systems; improved sensors, data acquisition and download speed.  It also doubled the science basket payload, and improved the command-and-control system allowing greater speed, range and maneuverability.

With seven reversible thrusters, it can hover in the water, maneuver over rugged topography, or rest on the sea floor.  It can collect data throughout the water column, produce a variety of maps and perform photographic surveys.  Alvin also has two robotic arms that can manipulate instruments, obtain samples, and its basket can be reconfigured daily based on the needs of the upcoming dive.

Alvin's depth rating of 6,500m gives researchers in-person access to 99% of the ocean floor.  Alvin is a proven and reliable platform capable of diving for up to 30 days in a row before requiring a single scheduled maintenance day.  Recent collaborations with autonomous vehicles such as Sentry have proven extremely beneficial, allowing PIs to visit promising sites to collect samples and data in person within hours of their being discovered, and UNOLs driven technological advances have improved the ability for scientific outreach and collaboration via telepresence

Alvin is named for Allyn Vine, a WHOI engineer and geophysicist who helped pioneer deep submergence research and technology.

(from https://www.whoi.edu/what-we-do/explore/underwater-vehicles/hov-alvin/, accessed 2022-09-09)</gco:CharacterString>
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        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/638.rdf" xlink:title="ROV Jason" xlink:actuate="onRequest">ROV JASON II</gmx:Anchor>
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          <gmi:type>
            <gco:CharacterString>ROV JASON II</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: ROV JASON II PI Supplied Instrument Description:Samples were collected using the HOV Alvin or ROV JASON II on oceanographic expeditions (cruises) AT42-09, AT42-21, and RR2102. Instrument Name: ROV Jason Instrument Short Name:ROV Jason   Instrument Description: The Remotely Operated Vehicle (ROV) Jason is operated by the Deep Submergence Laboratory (DSL) at Woods Hole Oceanographic Institution (WHOI). WHOI engineers and scientists designed and built the ROV Jason to give scientists access to the seafloor that didn't require them leaving the deck of the ship. Jason is a two-body ROV system. A 10-kilometer (6-mile) fiber-optic cable delivers electrical power and commands from the ship through Medea and down to Jason, which then returns data and live video imagery. Medea serves as a shock absorber, buffering Jason from the movements of the ship, while providing lighting and a bird’s eye view of the ROV during seafloor operations. During each dive (deployment of the ROV), Jason pilots and scientists work from a control room on the ship to monitor Jason’s instruments and video while maneuvering the vehicle and optionally performing a variety of sampling activities. Jason is equipped with sonar imagers, water samplers, video and still cameras, and lighting gear. Jason’s manipulator arms collect samples of rock, sediment, or marine life and place them in the vehicle’s basket or on &quot;elevator&quot; platforms that float heavier loads to the surface. More information is available from the operator site at URL. https://ndsf.whoi.edu/jason/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:operation>
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            <gmi:description>
              <gco:CharacterString>Cruise: AT42-09</gco:CharacterString>
            </gmi:description>
            <gmi:identifier>
              <gmd:MD_Identifier>
                <gmd:code>
                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/937024.rdf" xlink:title="Cruise" xlink:actuate="onRequest">AT42-09</gmx:Anchor>
                </gmd:code>
              </gmd:MD_Identifier>
            </gmi:identifier>
            <gmi:status>
              <gmd:MD_ProgressCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
            </gmi:status>
            <gmi:type>
              <gmi:MI_OperationTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MI_OperationTypeCode" codeListValue="real"/>
            </gmi:type>
            <gmi:parentOperation gco:nilReason="inapplicable"/>
            <gmi:platform>
  <gmi:MI_Platform>
    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://www.whoi.edu/page.do?pid=8143" xlink:actuate="onRequest">R/V Atlantis</gmx:Anchor>
       </gmd:title>
       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://vocab.nerc.ac.uk/collection/C17/current/33AT" xlink:actuate="onRequest">Community Standard Description</gmx:Anchor>
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       <gmd:date gco:nilReason="unknown"/>
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    </gmi:citation>
    <gmi:identifier>
      <gmd:MD_Identifier>
        <gmd:authority>
          <gmd:CI_Citation>
            <gmd:title>
              <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/authority/1.rdf" xlink:actuate="onRequest">International Council for the Exploration of the Sea</gmx:Anchor>
            </gmd:title>
            <gmd:date gco:nilReason="unknown"/>
          </gmd:CI_Citation>
        </gmd:authority>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54003.rdf"
           xlink:title="33AT" xlink:actuate="onRequest">R/V Atlantis</gmx:Anchor>
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    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54003.rdf" xlink:title="R/V Atlantis" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
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                      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/937024.rdf" xlink:title="Cruise" xlink:actuate="onRequest">AT42-09</gmx:Anchor>
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                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/565800.rdf" xlink:actuate="onRequest">Jason B. Sylvan</gmx:Anchor>
                        </gmd:individualName>
                        <gmd:organisationName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/64.rdf" xlink:title="Affiliation" xlink:actuate="onRequest">Texas A&amp;M University</gmx:Anchor>
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              </gmi:MI_Plan>
            </gmi:plan>
            </gmi:MI_Operation>
      </gmi:operation><gmi:operation>
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                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/879912.rdf" xlink:title="Cruise" xlink:actuate="onRequest">AT42-21</gmx:Anchor>
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              <gmd:MD_ProgressCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
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              <gmi:MI_OperationTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MI_OperationTypeCode" codeListValue="real"/>
            </gmi:type>
            <gmi:parentOperation gco:nilReason="inapplicable"/>
            <gmi:platform>
  <gmi:MI_Platform>
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     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://www.whoi.edu/page.do?pid=8143" xlink:actuate="onRequest">R/V Atlantis</gmx:Anchor>
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    </gmi:citation>
    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://vocab.nerc.ac.uk/collection/C17/current/33AT" xlink:actuate="onRequest">Community Standard Description</gmx:Anchor>
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          <gmd:CI_Citation>
            <gmd:title>
              <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/authority/1.rdf" xlink:actuate="onRequest">International Council for the Exploration of the Sea</gmx:Anchor>
            </gmd:title>
            <gmd:date gco:nilReason="unknown"/>
          </gmd:CI_Citation>
        </gmd:authority>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54003.rdf"
           xlink:title="33AT" xlink:actuate="onRequest">R/V Atlantis</gmx:Anchor>
        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54003.rdf" xlink:title="R/V Atlantis" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
</gmi:platform>
            <gmi:plan>
              <gmi:MI_Plan>
                <gmi:status>
                  <gmd:MD_ProgressCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
                </gmi:status>
                <gmi:citation>
                  <gmd:CI_Citation>
                    <gmd:title>
                      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/879912.rdf" xlink:title="Cruise" xlink:actuate="onRequest">AT42-21</gmx:Anchor>
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                      <gmd:CI_ResponsibleParty>
                      <gmd:individualName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/565800.rdf" xlink:actuate="onRequest">Jason B. Sylvan</gmx:Anchor>
                        </gmd:individualName>
                        <gmd:organisationName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/64.rdf" xlink:title="Affiliation" xlink:actuate="onRequest">Texas A&amp;M University</gmx:Anchor>
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      </gmi:operation><gmi:operation>
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              <gco:CharacterString>Cruise: RR2102</gco:CharacterString>
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            <gmi:identifier>
              <gmd:MD_Identifier>
                <gmd:code>
                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/879915.rdf" xlink:title="Cruise" xlink:actuate="onRequest">RR2102</gmx:Anchor>
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              <gmi:MI_OperationTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MI_OperationTypeCode" codeListValue="real"/>
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