http://lod.bco-dmo.org/id/dataset/989225 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2025-11-20 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Total dissolved nitrogen (TDN) and total dissolved phosphorus (TDP) of samples collected from rivers draining onto the West Florida Shelf and submarine groundwater wells on the Western coast of Florida from Nov 2022 to Mar 2024 2025-12-12 publication 2025-12-12 revision Woods Hole Oceanographic Institution (WHOI BCO-DMO) 2025-12-26 publication https://doi.org/10.26008/1912/bco-dmo.989225.1 Angela N. Knapp Florida State University principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Knapp, A. N. (2025) Total dissolved nitrogen (TDN) and total dissolved phosphorus (TDP) of samples collected from rivers draining onto the West Florida Shelf and submarine groundwater wells on the Western coast of Florida from Nov 2022 to Mar 2024. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-12-12 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.989225.1 [access date] STING Land TDN and TDP data Dataset Description: Methods and Sampling: &lt;p&gt;Riverine and estuarine samples were collected on land.&amp;nbsp;Submarine groundwater samples collected on various shore boats by Co-PI Christopher Smith. Riverine, estuarine and submarine groundwater well samples were collected between November 2022 and March 2024 using a trace metal clean pump and filtered through a 0.2 µm Pall Acropak Super membrane filter capsule, collected into 60 mL HDPE bottles and frozen at -20 C until analysis on land.&amp;nbsp;&lt;/p&gt; &lt;p&gt;TDP concentrations were measured using a Shimadzu UV-1800 and a Shimadzu UV-1900i. All reagents were prepared in dedicated labware with high purity Milli-Q (&amp;gt;18.2 MΩ cm) water. Samples were calibrated using a 6 point calibration curve with concentrations that bracketed sample concentrations. Each run included multiple Milli-Q water and reagent blanks, as well as three internal standards including adenosine triphosphate, a polyphosphate compound, glyphosate, a polyphosphonate compound, and 0.2&amp;nbsp;µm filtered oligotrophic surface water from the Gulf to check the consistency of the TDP concentration analysis over time.&lt;/p&gt; &lt;p&gt;TDN concentrations were measured using a Thermo 42i NOx analyzer and a Teledyne T200 NOx analyzer and were calibrated with standards that bracketed the concentration range of samples. Additionally, as an internal check on the completeness of the persulfate oxidation method, DON standards were included as “unknowns” in each set of persulfate oxidation, in this case USGS 40 (L-glutamic acid) and IAEA600 (caffeine).&amp;nbsp;&lt;/p&gt; &lt;p&gt;The detection limit for TDN concentration analysis is 0.1 µM, and detection limit for TDP concentration analysis is 0.025 µM.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2148989 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2148989 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2506248 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2506248 completed Angela N. Knapp Florida State University (979) 862-5235 797 Lamar St. Dept of Oceanography, Eller O&M Room 1204 College Station TX 77843 USA aknapp@tamu.edu pointOfContact asNeeded Dataset Version: 1 Unknown TYPE SAMPLE_ID STING_ID DATE STATION SAMPLE LATITUDE LONGITUDE TDP TDP_STDEV TDP_COUNT TDP_FLAG TDN TDN_STDEV TDN_COUNT TDN_FLAG Thermo 42i NOx analyzer Teledyne T200 NOx analyzer Trace metal clean pump Shimadzu UV-1800 Shimadzu UV-1900i theme None, User defined sample type sample identification date station latitude longitude Total Dissolved Phosphorus standard deviation count quality flag Dissolved Organic Nitrogen featureType BCO-DMO Standard Parameters Chemiluminescence NOx Analyzer Chemiluminescence NOx Analyzer Pump UV Spectrophotometer-Shimadzu UV Spectrophotometer-Shimadzu instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Linking iron and nitrogen sources in an oligotrophic coastal margin: Nitrogen fixation and the role of boundary fluxes https://osprey.bco-dmo.org/project/880516 Collaborative Research: Linking iron and nitrogen sources in an oligotrophic coastal margin: Nitrogen fixation and the role of boundary fluxes <p><strong>NSF Award Abstract:</strong><br /> This project will investigate how groundwater discharge delivers important nutrients to the coastal ecosystems of the West Florida Shelf. Preliminary studies indicate that groundwater may supply both dissolved organic nitrogen (DON) and iron in this region. In coastal ecosystems like the West Florida Shelf that have very low nitrate and ammonium concentrations, DON is the main form of nitrogen available to organisms. Nitrogen cycling is strongly affected by iron availability because iron is essential for both photosynthesis and for nitrogen fixation. This study will investigate the sources and composition of DON and iron, and their influence on the coastal ecosystem. The team will sample offshore groundwater wells, river and estuarine waters, and conduct two expeditions across the West Florida Shelf in winter and summer. Investigators will participate in K-12 and outreach activities to increase awareness of the project and related science. The project will fund the work of six graduate and eight undergraduate students across five institutions, furthering NSF’s goals of education and training.</p> <p>Motivated by preliminary observations of unexplained, tightly-correlated DON and dissolved iron concentrations across the West Florida Shelf (WFS), the proposed work will quantify the flux and isotopic signatures of submarine groundwater discharge (SGD)-derived DON and iron to the WFS, and evaluate the bioavailability of this temporally-variable source using four seasonal near-shore campaigns sampling offshore groundwater wells, estuarine, and riverine endmembers and two cross-shelf cruises. The work will evaluate whether SGD stimulates nitrogen fixation on the WFS, and the potential for the stimulated nitrogen fixation to further modify the chemistry of DON and dissolved iron in the region. The cross-shelf cruises will investigate hypothesized periods of maximum SGD and Trichodesmium abundance (June), and reduced river discharge and SGD (February), thus comparing two distinct biogeochemical regimes. The concentrations and isotopic compositions of DON and dissolved iron, molecular composition of DON, and the concentration and composition of iron-binding ligands will be characterized. Nitrogen fixation rates and Trichodesmium spp. abundance and expression of iron stress genes will be measured. Fluxes of DON and iron from SGD and rivers will be quantified with radium isotope mass balances. The impacts of SGD on nitrogen fixation and DON/ligand production will be constrained with incubations of natural phytoplankton communities with submarine groundwater amendments. Two hypotheses will be tested: 1) SGD is the dominant source of bioavailable DON and dissolved iron on the WFS, and 2) SGD-alleviation of iron stress changes the dominant Trichodesmium species on the WFS, increases nitrogen fixation rates and modifies DON and iron composition. Overall, the work will establish connections between marine nitrogen and iron cycling and evaluate the potential for coastal inputs to modify water along the WFS before export to the Atlantic Ocean. This study will thus provide a framework to consider these boundary fluxes in oligotrophic coastal systems and the relative importance of rivers and SGD as sources of nitrogen and iron in other analogous locations, such as coastal systems in Australia, India, and Africa, where nitrogen fixation and SGD have also been documented.</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> West Florida Shelf DON and Fe largerWorkCitation project eng; USA oceans -83.0012 -81.8474 26.3874 28.7669 2022-11-14 2024-03-08 Gulf of Mexico/America, West Florida Shelf 0 BCO-DMO catalogue of parameters from Total dissolved nitrogen (TDN) and total dissolved phosphorus (TDP) of samples collected from rivers draining onto the West Florida Shelf and submarine groundwater wells on the Western coast of Florida from Nov 2022 to Mar 2024 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/989624.rdf Name: TYPE Units: unitless Description: <p>Type of land based sampling: submarine groundwater well (WELL), river (RIVER), or seawater (SW).</p> http://lod.bco-dmo.org/id/dataset-parameter/989625.rdf Name: SAMPLE_ID Units: unitless Description: <p>Land-based sample identifier</p> http://lod.bco-dmo.org/id/dataset-parameter/989626.rdf Name: STING_ID Units: unitless Description: <p>Unique number assigned to each sampling event in STING project</p> http://lod.bco-dmo.org/id/dataset-parameter/989627.rdf Name: DATE Units: unitless Description: <p>Date the sample was collected; ‘NDA’ for ‘no data available’  or missing information</p> http://lod.bco-dmo.org/id/dataset-parameter/989628.rdf Name: STATION Units: unitless Description: <p>USGS station identifier for submarine ground water well samples, and station name for river and estuary samples; &#039;VH&#039; = Venice Headlands transect; &#039;IRB&#039; = Indian Rocks Beach transect; &#039;NC&#039; = Nature Coast transect</p> http://lod.bco-dmo.org/id/dataset-parameter/989629.rdf Name: SAMPLE Units: unitless Description: <p>Type of sample, where &#039;SW&#039; stands for &#039;surface water sample collected over a submarine groundwater well&#039;; &#039;BW&#039; stands for &#039;water collected just above the sediment/water interface about a submarine groundwater well&#039;, and &#039;GD&#039; and &#039;GS&#039; stand for &#039;water collected from a submarine groundwater well&#039;; &#039;BW Fe&#039; and &#039;SW Fe&#039; indicate these were either &#039;Bottom Water&#039; (BW) or &#039;Surface water&#039; (SW) seawater samples that were collected using the same methods as samples collected for iron (Fe) and other trace metal analysis</p> http://lod.bco-dmo.org/id/dataset-parameter/989631.rdf Name: LATITUDE Units: decimal degrees Description: <p>Position when sample was collected in decimal degrees; a positive value indicates a Northern coordinate; ‘NDA’ for ‘no data available’  or missing information</p> http://lod.bco-dmo.org/id/dataset-parameter/989633.rdf Name: LONGITUDE Units: decimal degrees Description: <p>Position when sample was collected in decimal degrees; a negative value indicates a Western coordinate; ‘NDA’ for ‘no data available’  or missing information</p> http://lod.bco-dmo.org/id/dataset-parameter/989635.rdf Name: TDP Units: micromoles per liter (µM) Description: <p>Concentrations of total dissolved phosphorus</p> http://lod.bco-dmo.org/id/dataset-parameter/989636.rdf Name: TDP_STDEV Units: micromoles per liter (µM) Description: <p>Standard deviation of replication total dissolved phosphorus measurements. If only 2 replicates, the difference about the mean was used to calculate error</p> http://lod.bco-dmo.org/id/dataset-parameter/989637.rdf Name: TDP_COUNT Units: unitless Description: <p>Number of TDP analytical replicates performed</p> http://lod.bco-dmo.org/id/dataset-parameter/989638.rdf Name: TDP_FLAG Units: unitless Description: <p>Quality flag for TDP measurements. See the &#039;Data Processing Description&#039; section of the BCO-DMO metadata page for this dataset for full data quality flag details</p> http://lod.bco-dmo.org/id/dataset-parameter/989639.rdf Name: TDN Units: micromoles per liter (µM) Description: <p>Concentrations of total dissolved nitrogen</p> http://lod.bco-dmo.org/id/dataset-parameter/989640.rdf Name: TDN_STDEV Units: micromoles per liter (µM) Description: <p>Standard deviation of replication total dissolved nitrogen measurements. If only 2 replicates, the difference about the mean was used to calculate error</p> http://lod.bco-dmo.org/id/dataset-parameter/989641.rdf Name: TDN_COUNT Units: unitless Description: <p>Number of TDN analytical replicates performed</p> http://lod.bco-dmo.org/id/dataset-parameter/989642.rdf Name: TDN_FLAG Units: unitless Description: <p>Quality flag for TDN measurements. See the &#039;Data Processing Description&#039; section of the BCO-DMO metadata page for this dataset for full data quality flag details</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact https://doi.org/10.26008/1912/bco-dmo.989225.1 download onLine dataset &lt;p&gt;Riverine and estuarine samples were collected on land.&amp;nbsp;Submarine groundwater samples collected on various shore boats by Co-PI Christopher Smith. Riverine, estuarine and submarine groundwater well samples were collected between November 2022 and March 2024 using a trace metal clean pump and filtered through a 0.2 µm Pall Acropak Super membrane filter capsule, collected into 60 mL HDPE bottles and frozen at -20 C until analysis on land.&amp;nbsp;&lt;/p&gt; &lt;p&gt;TDP concentrations were measured using a Shimadzu UV-1800 and a Shimadzu UV-1900i. All reagents were prepared in dedicated labware with high purity Milli-Q (&amp;gt;18.2 MΩ cm) water. Samples were calibrated using a 6 point calibration curve with concentrations that bracketed sample concentrations. Each run included multiple Milli-Q water and reagent blanks, as well as three internal standards including adenosine triphosphate, a polyphosphate compound, glyphosate, a polyphosphonate compound, and 0.2&amp;nbsp;µm filtered oligotrophic surface water from the Gulf to check the consistency of the TDP concentration analysis over time.&lt;/p&gt; &lt;p&gt;TDN concentrations were measured using a Thermo 42i NOx analyzer and a Teledyne T200 NOx analyzer and were calibrated with standards that bracketed the concentration range of samples. Additionally, as an internal check on the completeness of the persulfate oxidation method, DON standards were included as “unknowns” in each set of persulfate oxidation, in this case USGS 40 (L-glutamic acid) and IAEA600 (caffeine).&amp;nbsp;&lt;/p&gt; &lt;p&gt;The detection limit for TDN concentration analysis is 0.1 µM, and detection limit for TDP concentration analysis is 0.025 µM.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;Data were flagged using the SeaDataNet quality flag scheme recommended by GEOTRACES (https://www.geotraces.org/geotraces-quality-flag-policy/)&amp;nbsp;and described below. Notes specific to the application of these flags to this dataset are noted in brackets […].&lt;/p&gt; &lt;p&gt;0: No Quality Control: No quality control procedures have been applied to the data value. This is the initial status for all data values entering the working archive. [Not used].&lt;/p&gt; &lt;p&gt;1: Good Value: Good quality data value that is not part of any identified malfunction and has been verified as consistent with real phenomena during the quality control process.&lt;/p&gt; &lt;p&gt;2: Probably Good Value: Data value that is probably consistent with real phenomena, but this is unconfirmed or data value forming part of a malfunction that is considered too small to affect the overall quality of the data object of which it is a part. [Used when no replicate measurements were available to check the quality of the data].&lt;/p&gt; &lt;p&gt;3: Probably Bad Value: Data value recognized as unusual during quality control that forms part of a feature that is probably inconsistent with real phenomena. [Used when all replicate measurements were too high to be consistent with real phenomena].&lt;/p&gt; &lt;p&gt;4: Bad Value: An obviously erroneous data value. [Not used].&lt;/p&gt; &lt;p&gt;5: Changed Value: Data value adjusted during quality control. Best practice strongly recommends that the value before the change be preserved in the data or its accompanying metadata. [Not used].&lt;/p&gt; &lt;p&gt;6: Value Below Detection Limit: The level of the measured phenomenon was less than the limit of detection (LOD) for the method employed to measure it. The accompanying value is the detection limit for the technique or zero if that value is unknown. [Values below detection are reported as 0.00 µM in the data file. Detection limits for each parameter are listed in the “methods and sampling” section of this form].&lt;/p&gt; &lt;p&gt;7: Value in Excess: The level of the measured phenomenon was too large to be quantified by the technique employed to measure it. The accompanying value is the measurement limit for the technique. [Not used].&lt;/p&gt; &lt;p&gt;8: Interpolated Value: This value has been derived by interpolation from other values in the data object. [Not used].&lt;/p&gt; &lt;p&gt;9: Missing Value: The data value is missing. Any accompanying value will be a magic number representing absent data [When sample was not collected the notation ‘na’ for ‘not applicable’ was used; when sample collected but there is no result for this parameter, the notation ‘NDA’ for ‘no data available’ was used].&lt;/p&gt; &lt;p&gt;A: Value Phenomenon Uncertain: There is uncertainty in the description of the measured phenomenon associated with the value such as chemical species or biological entity. [Not used.]&lt;/p&gt; Specified by the Principal Investigator(s) - Opened &quot;BCO-DMO_STING_LandSampling_TDN_TDP_Conc_v3.xlsx&quot; in Excel and replaced #REF errors with intended sample ID - Saved file as &quot;BCO-DMO_STING_LandSampling_TDN_TDP_Conc_v3_DM.xlsx&quot; and uploaded the file again to the submission - Imported &quot;BCO-DMO_STING_LandSampling_TDN_TDP_Conc_v3_DM.xlsx&quot; into the BCO-DMO system - Exported file as &quot;989225_v1_sting_land_tdn_tdp.csv&quot; Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Thermo 42i NOx analyzer Thermo 42i NOx analyzer PI Supplied Instrument Name: Thermo 42i NOx analyzer PI Supplied Instrument Description:TDN concentrations were measured using a Thermo 42i NOx analyzer and a Teledyne T200 NOx analyzer and were calibrated with standards that bracketed the concentration range of samples. Instrument Name: Chemiluminescence NOx Analyzer Instrument Short Name: Instrument Description: The chemiluminescence method for gas analysis of oxides of nitrogen relies on the measurement of light produced by the gas-phase titration of nitric oxide and ozone. A chemiluminescence analyzer can measure the concentration of NO/NO2/NOX. One example is the Teledyne Model T200: https://www.teledyne-api.com/products/nitrogen-compound-instruments/t200 Teledyne T200 NOx analyzer Teledyne T200 NOx analyzer PI Supplied Instrument Name: Teledyne T200 NOx analyzer PI Supplied Instrument Description:TDN concentrations were measured using a Thermo 42i NOx analyzer and a Teledyne T200 NOx analyzer and were calibrated with standards that bracketed the concentration range of samples. Instrument Name: Chemiluminescence NOx Analyzer Instrument Short Name: Instrument Description: The chemiluminescence method for gas analysis of oxides of nitrogen relies on the measurement of light produced by the gas-phase titration of nitric oxide and ozone. A chemiluminescence analyzer can measure the concentration of NO/NO2/NOX. One example is the Teledyne Model T200: https://www.teledyne-api.com/products/nitrogen-compound-instruments/t200 Trace metal clean pump Trace metal clean pump PI Supplied Instrument Name: Trace metal clean pump PI Supplied Instrument Description:Riverine, estuarine and submarine groundwater well samples were collected using a trace metal clean pump and filtered through a 0.2 µm Pall Acropak Super membrane filter capsule, collected into 60 mL HDPE bottles and frozen at -20 C until analysis on land.  Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps Shimadzu UV-1800 Shimadzu UV-1800 PI Supplied Instrument Name: Shimadzu UV-1800 PI Supplied Instrument Description:TDP concentrations were measured using a Shimadzu UV-1800 and a Shimadzu UV-1900i.  Instrument Name: UV Spectrophotometer-Shimadzu Instrument Short Name:UV Spectrophotometer-Shimadzu Instrument Description: The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ Shimadzu UV-1900i Shimadzu UV-1900i PI Supplied Instrument Name: Shimadzu UV-1900i PI Supplied Instrument Description:TDP concentrations were measured using a Shimadzu UV-1800 and a Shimadzu UV-1900i.  Instrument Name: UV Spectrophotometer-Shimadzu Instrument Short Name:UV Spectrophotometer-Shimadzu Instrument Description: The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/