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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/994284.rdf" xlink:actuate="onRequest">Results of imaging flow cytobot (IFCB) analysis of biovolumes of key phytoplankton taxa from waters collected near study sites along the rocky intertidal on the Oregon coast in 2023 and 2024</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Menge, B. A., Rothman, B., Vinton, A. (2026) Results of imaging flow cytobot (IFCB) analysis of biovolumes of key phytoplankton taxa from waters collected near study sites along the rocky intertidal on the Oregon coast in 2023 and 2024. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-04-02 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/994284 [access date]</gco:CharacterString>
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        <gco:CharacterString>IFCB analysis of biovolumes of key phytoplankton taxa Dataset Description:  Methods and Sampling: &amp;lt;p&amp;gt;Collection and analysis of &amp;quot;live&amp;quot; (unprocessed) water samples and separate preserved water samples for assessment of potential toxin-producing phytoplankton was done monthly. Samples were processed by Dr. Eli Meyer of Aquabiomics Inc. (Junction City, OR), by Dr. Ralph Elston of Aquatechnics (Carlsborg, WA), by Dr. Maria Kavanaugh of OSU's College of Earth, Ocean, and Atmospheric Sciences (Seascape Ecology Lab), and Dr. Manuel Garcia-Jaramillo in the Department of Environmental and Molecular Toxicology at OSU.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;IFCB sampling following Dr. Kavanaugh's protocol: replicate (n = 3) bottle samples were collected at each site in September and November 2023, and in January, February, and June 2024. Dataset rows are from replicate bottle samples. Live samples are allowed to settle, then processed using Image FlowCytoBot (IFCB) sampling for the presence and abundance of preservation-sensitive phytoplankton (e.g., &amp;quot;naked&amp;quot; flagellates). Hardier plankters can be examined in both live and preserved samples.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Dataset rows: Each row corresponds to an instrument run. Generally, that would be one per sample bottle, but on occasion, samples would be run in duplicate (e.g., if the instrument had an issue with the first run), in which case a single sample might be accounted for in more than one row. The date is embedded in the File ID.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/910809.rdf" xlink:title="OCE-2346837" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2346837 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2346837</gmx:Anchor>
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On temperate rocky shores, mussels are a 'foundation' species, serving as prey for multiple predators, able to filter particles out of huge volumes of water, and harboring hundreds of other species, thereby serving a central role in community function. Hence, threats to their abundance and persistence are of significant ecological and societal concern. Past research has shown that mussels are highly resilient and well-adapted to acute, short-lived stresses such as disturbance from winter storms or short-term warming. In summer 2023, a novel pattern of mortality was discovered during routine field research: high numbers of dead mussels, many with tissue remaining in the shell were observed in a scattered pattern. That is, rather than a mass mortality (i.e., all mussels in a large area are killed), single dead mussels surrounded by live and apparently healthy mussels occurred, with multiple dead individuals per square meter. This project investigates the cause of the mortality event, which preliminary data suggests may be a harmful algal bloom. The project also builds capacity by supporting research training for several undergraduate students and informs the public and resource managers about an emerging threat to the stability of rocky intertidal ecosystems.&lt;/p&gt;
&lt;p&gt;The hypothesis under investigation is that the 2023 'scattered' mortality event at 8 study sites along the Oregon and northern California coasts affecting the rocky intertidal dominant, &lt;em&gt;Mytilus californianus&lt;/em&gt;, may be an unusual consequence of a harmful algal bloom. During this event, densities of dead mussels have ranged from 0.7 to 10.6/m2. This novel pattern contrasts with typical mass mortalities of organisms where all individuals within a specific site or area die due to (e.g.) thermal stress or severe storms. The scattered mortality pattern persisted through late September 2023. Preliminary histological evidence suggests the ability of mussels to digest food is being suppressed, most likely by a water-borne toxin. To investigate the cause of the mortality event, the team is (1) collecting monthly (a) water samples for identification of dinoflagellate species and (b) mussel tissue samples for histological investigation of digestive and kidney tissues and high performance liquid chromatography and mass spectrometry analyses for the presence of algal-derived toxins; (2) continuing mussel bed surveys to determine the seasonality of the mortality event; and (3) quantifying mussel bed persistence through winter storms at sites with higher or lower rates of mortality. This research advances understanding of community responses to intense environmental stresses and how these might influence the future of rocky intertidal ecosystems.&lt;/p&gt;</gco:CharacterString>
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	Description: &lt;p&gt;Indicates live or preserved samples&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/995869.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/995871.rdf
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	Description: &lt;p&gt;Sampling site latitude&lt;/p&gt; 
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http://lod.bco-dmo.org/id/dataset-parameter/995873.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/995874.rdf
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	Description: &lt;p&gt;TBD?&lt;/p&gt; 
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http://lod.bco-dmo.org/id/dataset-parameter/995878.rdf
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	Units: cubic microns per milliliter
	Description: &lt;p&gt;Diatom biovolume&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/995879.rdf
	Name: Dinoflagellate_microns3_ml
	Units: cubic microns per milliliter
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http://lod.bco-dmo.org/id/dataset-parameter/995880.rdf
	Name: Coccolithophore_microns3_ml
	Units: cubic microns per milliliter
	Description: &lt;p&gt;Coccolithophore biovolume&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/995881.rdf
	Name: Ciliate_microns3_ml
	Units: cubic microns per milliliter
	Description: &lt;p&gt;Ciliate biovolume&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/995882.rdf
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	Units: cubic microns per milliliter
	Description: &lt;p&gt;OtherPhyto the IFCB&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/995883.rdf
	Name: OtherMixoHet_microns3_ml
	Units: cubic microns per milliliter
	Description: &lt;p&gt;OtherMixoHet the IFCB&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/995884.rdf
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	Units: cubic microns per milliliter
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http://lod.bco-dmo.org/id/dataset-parameter/995885.rdf
	Name: Prymnesiophyte_microns3_ml
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http://lod.bco-dmo.org/id/dataset-parameter/995886.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/995887.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/995888.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/995889.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/995890.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/995892.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/995893.rdf
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                  <gco:CharacterString>- Imported site metadata from &amp;quot;Copy of 6590_menge_site_descrip.xlsx&amp;quot; (sheet 1) into the BCO-DMO system.
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- Renamed fields to comply with BCO-DMO naming conventions.
- Joined the site list to the IFCB biovolume data matching on site code, adding fields: &amp;quot;site_lat&amp;quot;, &amp;quot;site_lon&amp;quot;, and &amp;quot;site_name&amp;quot;.
-  Converted &amp;quot;date&amp;quot; field from format &amp;quot;%m-%d-%y&amp;quot; to ISO date format &amp;quot;%Y-%m-%d&amp;quot;.
- Converted &amp;quot;Date_Run_on_IFCB&amp;quot; from format &amp;quot;%m/%d/%Y&amp;quot; to ISO date format &amp;quot;%Y-%m-%d&amp;quot;.
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