http://lod.bco-dmo.org/id/dataset/995155 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2026-03-18 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Coral physiological and biological data collected during diel sampling in outdoor mesocosms in Hawaii from January 2022 to March 2024 2026-03-18 publication 2026-03-18 revision BCO-DMO Linked Data URI 2026-03-18 creation http://lod.bco-dmo.org/id/dataset/995155 Keisha D. Bahr Texas A&M, Corpus Christi principalInvestigator Christopher L. Sabine University of Hawaiʻi at Mānoa principalInvestigator Conall McNicholl Hawaii Institute of Marine Biology principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Bahr, K. D., McNicholl, C., Sabine, C. L., Armstrong, D. A., Bretzing-Tungate, R. (2026) Coral physiological and biological data collected during diel sampling in outdoor mesocosms in Hawaii from January 2022 to March 2024. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-03-18 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/995155 [access date] Methods and Sampling: &lt;p&gt;&lt;strong&gt;Overview&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Seasonal mesocosm incubations of Montipora capitata and Pocillopora acuta were conducted at the Hawai‘i Institute of Marine Biology (HIMB) from June 2022 through March 2024. Experiments were run during two seasonal windows each year: winter (January 10–March 17) and summer (June 10–August 17). Due to facility limitations, all treatments could not run simultaneously. Therefore, each seasonal experiment consisted of two sequential 30-day exposures, each using newly collected coral colonies. At the beginning (Day 0) and end (Day 30) of each experiment, a 26-hour diel sampling was conducted to characterize variability in total alkalinity (TA) and carbonate system parameters under each treatment. All methods were standardized across replicates, with only TA and pH manipulations differing among treatments.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Coral Collection and Pre-Experiment Holding&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Coral colonies of M. capitata and P. acuta were collected by hand while snorkeling from shallow (1 m) fringing reef habitat surrounding HIMB in Kāne‘ohe Bay, Hawai‘i. Colonies were transported immediately to the HIMB mesocosm facility and held for one week in flow-through tanks identical to the experimental mesocosms. One day before each 30-day experiment, colonies were stained with alizarin red for eight hours to mark the starting skeletal growth band (Jokiel and Morrissey 1993). At the start of each experiment, colonies were buoyant weighed following Jokiel (1978) and randomly assigned to mesocosms. Each mesocosm contained 20 colonies of each species arranged in a standardized alternating layout to avoid species-level clustering.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Mesocosm Facility Design&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Experiments were conducted in a flow-through mesocosm facility described by Jokiel et al. (2014). The system consisted of twelve 450 L fiberglass tanks arranged in four rows of three mesocosms, each row supplied by a dedicated header tank. Natural seawater was pumped from Kāne‘ohe Bay at ~3 m depth and delivered unfiltered to preserve natural diel and seasonal environmental variability. Each header tank fed seawater into a 100 L mixing reservoir where TA and pH manipulations were applied before seawater entered the mesocosms. Residence time in each mesocosm was greater than one hour. Each tank contained two submersible circulation pumps and a continuously running airstone to maintain internal water movement and oxygenation.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Treatment Conditions and Carbonate Chemistry Manipulation&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Four carbonate chemistry treatments were created across each pair of 30-day experiments through controlled manipulation of total alkalinity (TA), dissolved inorganic carbon (DIC), and pH:&lt;/p&gt; &lt;p&gt;Ambient control&lt;/p&gt; &lt;p&gt;Low pH (target ΔpH approximately −0.3 from ambient)&lt;/p&gt; &lt;p&gt;High or low TA (±100 µmol kg−1 from ambient)&lt;/p&gt; &lt;p&gt;Combined low pH and altered TA&lt;/p&gt; &lt;p&gt;TA manipulations were accomplished in header mixing tanks using peristaltic pumps delivering either 1.0 M HCl (for low TA; ~3 mL min−1) or 1.0 M Na2CO3 (for high TA; ~2 mL min−1). pH reductions were achieved by bubbling pure CO2 or a CO2-air mixture directly into mesocosms using a Maxi-Jet 1600 pump-driven venturi injector. Because each row was supplied by a single header tank, only one direction of TA manipulation (increase or decrease) could be performed within a given 30-day period. The second 30-day experiment used the opposite TA manipulation to ensure all treatments were completed each season.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Seawater Sampling and Measurements&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Daily Measurements&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Temperature, salinity, dissolved oxygen, and pHNBS were measured daily at mid-day in each mesocosm and header tank. During diel sampling, these parameters were measured every hour on the hour for 26 consecutive hours. Measurements were collected using a YSI ProDSS or YSI 556 MPS multiparameter meter.&lt;/p&gt; &lt;p&gt;pH electrodes were calibrated daily using NIST-traceable pH 4, 7, and 10 buffers and corrected to the total scale using Tris buffer from the A. Dickson laboratory (Scripps Institution of Oceanography). Dissolved oxygen probe calibrations followed manufacturer air-saturation protocols.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Total Alkalinity Sampling and Storage&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Seawater samples for TA analysis were collected hourly during each 26-hour diel cycle. Bottles were rinsed three times with sample water and filled in acid-cleaned 100 mL borosilicate bottles with no headspace to prevent gas exchange. Samples were stored in the dark at ambient temperature and analyzed within 12 hours of collection. Sampling followed best practices described in Dickson et al. (2007).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;TA Determination and Derived Carbonate Chemistry&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Total alkalinity was measured by open-cell potentiometric titration on a Metrohm 877 Titrino Plus with a Metrohm 9101 Herisau glass pH electrode. All titrations were standardized using certified reference materials (CRMs) from the Dickson laboratory. Electrode slope, offset, and drift were checked daily to ensure analytical consistency. Carbonate system parameters, including DIC, pCO2, bicarbonate, carbonate ion concentration, and omega aragonite, were calculated using the R package seacarb.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2049406 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2049406 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2049407 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2049407 onGoing Keisha D. Bahr Texas A&M, Corpus Christi 361-825-3707 6300 Ocean Drive, Unit 5800 Corpus Christi TX 78412-5844 USA Keisha.Bahr@tamucc.edu pointOfContact Christopher L. Sabine University of Hawaiʻi at Mānoa Department of Oceanography 1000 Pope Road Honolulu HI 96822 USA csabine@hawaii.edu pointOfContact Conall McNicholl Hawaii Institute of Marine Biology cmcnic@hawaii.edu pointOfContact asNeeded Dataset Version: 1 Unknown date experiment tp season treatment type mesocosm tank_area tank_depth tod flow_rate ta temp sal o2 ph_nbs Snorkel-based Coral Collection Gear Flow-through Mesocosm System CO2 Gas Delivery System Submersible Pumps Peristaltic Pumps for TA Manipulation Buoyant Weighing Apparatus Open-cell Potentiometric Titrator for Total Alkalinity YSI Multiparameter Water Quality Meters theme None, User defined No BCO-DMO term experiment id sample description treatment sample type tank surface_area flow rate total alkalinity (TA) temperature of sample or a generic measurement (not in-situ water temp) salinity dissolved Oxygen pH featureType BCO-DMO Standard Parameters Diving Mask and Snorkel no_bcodmo_term no_bcodmo_term Pump Pump scale or balance Titrator Water Quality Multiprobe instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Reevaluating calcification response to changes in seawater chemistry by testing the Proton Flux Hypothesis and the Coral Metabolism Model https://osprey.bco-dmo.org/project/868178 Collaborative Research: Reevaluating calcification response to changes in seawater chemistry by testing the Proton Flux Hypothesis and the Coral Metabolism Model <p><em>NSF Award Abstract:</em><br /> Corals build calcium carbonate skeletons to maintain the three-dimensional structure of a coral reef, which provides habitat for many organisms and protects shorelines from bioerosion and storm damage. However, changes in ocean chemistry threaten the ability of corals to build and sustain these ecological important structures. To further the understanding of how climate change impacts coral reefs, this project investigates how changes in ocean carbonate chemistry directly influence coral calcification. The researchers are conducting a series of experiments on corals grown in seawater tanks to study corals responses to seawater chemistry in a changing ocean. Broader impacts of the project include student research opportunities, science-inquiry labs, and virtual learning. This project supports the training of several early career researchers, Ph.D. students, undergraduates, and high school students in the disciplines of chemistry, engineering, and marine ecology. Researchers partner with the Texas State Aquarium to communicate with the general public through a virtual research expedition series that will focus on coral reef health. This series includes interviews, behind the scene tours, and virtual dives on coral reefs in Hawaii.</p> <p>This project examines the fundamental connections between seawater chemistry and coral physiology by investigating the modulation of seawater chemistry in the microenvironment surrounding corals. Specifically, this project 1) examines the response of corals to differing carbonate chemistry and 2) characterizes the proton gradient across the corals' boundary layers under differing ocean acidification conditions. Results of this work isolate whether carbonate ions or hydrogen ions have a stronger influence on calcification rates. This work utilizes a state-of-the-art experimental mesocosm facility that combines an automated systems to simultaneously and independently control both total alkalinity and carbon dioxide in the tanks to examine coral response under different carbon chemistry scenarios. Small-scale gradients in carbon chemistry surrounding the corals are being characterized using an innovative solid-state, reagentless sensor capable of making simultaneous measurements of two critical carbon system parameters. Coral biological response variables are quantified during short-term incubations and long-term mesocosm manipulations to understand physiological implications across multiple scales (i.e., individual and community scales) and across boundary layers.</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> Proton Flux Hypothesis largerWorkCitation project eng; USA oceans -157.7877 -157.7877 21.4327 21.4327 2022-06-07 2024-03-15 Kaneohe, Hawaii 0 BCO-DMO catalogue of parameters from Coral physiological and biological data collected during diel sampling in outdoor mesocosms in Hawaii from January 2022 to March 2024 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/995832.rdf Name: date Units: units Description: <p>description</p> http://lod.bco-dmo.org/id/dataset-parameter/995833.rdf Name: experiment Units: unitless Description: <p>Identifier for each mesocosm experiment conducted between 2022 and 2024</p> http://lod.bco-dmo.org/id/dataset-parameter/995834.rdf Name: tp Units: unitless Description: <p>Timepoint indicator describing sampling day within the experiment (e.g., Day 0 or Day 30)</p> http://lod.bco-dmo.org/id/dataset-parameter/995835.rdf Name: season Units: unitless Description: <p>Season during which the experiment was conducted (e.g., summer, winter)</p> http://lod.bco-dmo.org/id/dataset-parameter/995836.rdf Name: treatment Units: unitless Description: <p>Carbonate chemistry treatment applied (ambient control or elevated pCO2)</p> http://lod.bco-dmo.org/id/dataset-parameter/995837.rdf Name: type Units: unitless Description: <p>Sampling type indicating diurnal sampling effort</p> http://lod.bco-dmo.org/id/dataset-parameter/995838.rdf Name: mesocosm Units: unitless Description: <p>Identifier of the mesocosm unit from which measurements were taken</p> http://lod.bco-dmo.org/id/dataset-parameter/995839.rdf Name: tank_area Units: square meters (m2) Description: <p>Surface area of the mesocosm tank</p> http://lod.bco-dmo.org/id/dataset-parameter/995840.rdf Name: tank_depth Units: meters (m) Description: <p>Depth of the mesocosm tank</p> http://lod.bco-dmo.org/id/dataset-parameter/995841.rdf Name: tod Units: unitless Description: <p>Time of day of measurement (e.g., morning, midday, evening)</p> http://lod.bco-dmo.org/id/dataset-parameter/995842.rdf Name: flow_rate Units: liters per second (L sec-1) Description: <p>Flow rate of seawater entering the mesocosm</p> http://lod.bco-dmo.org/id/dataset-parameter/995843.rdf Name: ta Units: micromoles per kilogram (umol kg -1) Description: <p>Total alkalinity of seawater</p> http://lod.bco-dmo.org/id/dataset-parameter/995844.rdf Name: temp Units: degrees Celsius Description: <p>Seawater temperature</p> http://lod.bco-dmo.org/id/dataset-parameter/995845.rdf Name: sal Units: [parts per thousand (ppt)?] Description: <p>Seawater salinity</p> http://lod.bco-dmo.org/id/dataset-parameter/995846.rdf Name: o2 Units: miligrams per liter (mg/L) Description: <p>Dissolved oxygen concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/995847.rdf Name: ph_nbs Units: NBS scale Description: <p>pH measured on the NBS scale</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact https://osprey.bco-dmo.org/dataset/995155/data/download download onLine dataset &lt;p&gt;&lt;strong&gt;Overview&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Seasonal mesocosm incubations of Montipora capitata and Pocillopora acuta were conducted at the Hawai‘i Institute of Marine Biology (HIMB) from June 2022 through March 2024. Experiments were run during two seasonal windows each year: winter (January 10–March 17) and summer (June 10–August 17). Due to facility limitations, all treatments could not run simultaneously. Therefore, each seasonal experiment consisted of two sequential 30-day exposures, each using newly collected coral colonies. At the beginning (Day 0) and end (Day 30) of each experiment, a 26-hour diel sampling was conducted to characterize variability in total alkalinity (TA) and carbonate system parameters under each treatment. All methods were standardized across replicates, with only TA and pH manipulations differing among treatments.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Coral Collection and Pre-Experiment Holding&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Coral colonies of M. capitata and P. acuta were collected by hand while snorkeling from shallow (1 m) fringing reef habitat surrounding HIMB in Kāne‘ohe Bay, Hawai‘i. Colonies were transported immediately to the HIMB mesocosm facility and held for one week in flow-through tanks identical to the experimental mesocosms. One day before each 30-day experiment, colonies were stained with alizarin red for eight hours to mark the starting skeletal growth band (Jokiel and Morrissey 1993). At the start of each experiment, colonies were buoyant weighed following Jokiel (1978) and randomly assigned to mesocosms. Each mesocosm contained 20 colonies of each species arranged in a standardized alternating layout to avoid species-level clustering.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Mesocosm Facility Design&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Experiments were conducted in a flow-through mesocosm facility described by Jokiel et al. (2014). The system consisted of twelve 450 L fiberglass tanks arranged in four rows of three mesocosms, each row supplied by a dedicated header tank. Natural seawater was pumped from Kāne‘ohe Bay at ~3 m depth and delivered unfiltered to preserve natural diel and seasonal environmental variability. Each header tank fed seawater into a 100 L mixing reservoir where TA and pH manipulations were applied before seawater entered the mesocosms. Residence time in each mesocosm was greater than one hour. Each tank contained two submersible circulation pumps and a continuously running airstone to maintain internal water movement and oxygenation.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Treatment Conditions and Carbonate Chemistry Manipulation&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Four carbonate chemistry treatments were created across each pair of 30-day experiments through controlled manipulation of total alkalinity (TA), dissolved inorganic carbon (DIC), and pH:&lt;/p&gt; &lt;p&gt;Ambient control&lt;/p&gt; &lt;p&gt;Low pH (target ΔpH approximately −0.3 from ambient)&lt;/p&gt; &lt;p&gt;High or low TA (±100 µmol kg−1 from ambient)&lt;/p&gt; &lt;p&gt;Combined low pH and altered TA&lt;/p&gt; &lt;p&gt;TA manipulations were accomplished in header mixing tanks using peristaltic pumps delivering either 1.0 M HCl (for low TA; ~3 mL min−1) or 1.0 M Na2CO3 (for high TA; ~2 mL min−1). pH reductions were achieved by bubbling pure CO2 or a CO2-air mixture directly into mesocosms using a Maxi-Jet 1600 pump-driven venturi injector. Because each row was supplied by a single header tank, only one direction of TA manipulation (increase or decrease) could be performed within a given 30-day period. The second 30-day experiment used the opposite TA manipulation to ensure all treatments were completed each season.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Seawater Sampling and Measurements&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Daily Measurements&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Temperature, salinity, dissolved oxygen, and pHNBS were measured daily at mid-day in each mesocosm and header tank. During diel sampling, these parameters were measured every hour on the hour for 26 consecutive hours. Measurements were collected using a YSI ProDSS or YSI 556 MPS multiparameter meter.&lt;/p&gt; &lt;p&gt;pH electrodes were calibrated daily using NIST-traceable pH 4, 7, and 10 buffers and corrected to the total scale using Tris buffer from the A. Dickson laboratory (Scripps Institution of Oceanography). Dissolved oxygen probe calibrations followed manufacturer air-saturation protocols.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Total Alkalinity Sampling and Storage&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Seawater samples for TA analysis were collected hourly during each 26-hour diel cycle. Bottles were rinsed three times with sample water and filled in acid-cleaned 100 mL borosilicate bottles with no headspace to prevent gas exchange. Samples were stored in the dark at ambient temperature and analyzed within 12 hours of collection. Sampling followed best practices described in Dickson et al. (2007).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;TA Determination and Derived Carbonate Chemistry&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Total alkalinity was measured by open-cell potentiometric titration on a Metrohm 877 Titrino Plus with a Metrohm 9101 Herisau glass pH electrode. All titrations were standardized using certified reference materials (CRMs) from the Dickson laboratory. Electrode slope, offset, and drift were checked daily to ensure analytical consistency. Carbonate system parameters, including DIC, pCO2, bicarbonate, carbonate ion concentration, and omega aragonite, were calculated using the R package seacarb.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;&lt;strong&gt;Overview&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Data processing included organization of raw seawater chemistry measurements, conversion of pH values to the total scale, calculation of carbonate system parameters, buoyant-weight-based calcification calculations, and post-hoc biochemical and skeletal data extraction. All steps described below represent the procedures used to generate the submitted dataset. No statistical analysis, modeling, or graphing is included here.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Seawater Chemistry Data Processing&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;&lt;strong&gt;pH Conversion&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Daily pHNBS measurements from the YSI multiparameter meter were converted to total scale (pHT) using Tris buffer reference standards prepared and certified by the Dickson Laboratory. Temperature-dependent conversion equations followed Dickson et al. (2007).&lt;br /&gt; All pH values were standardized across seasons using:&lt;/p&gt; &lt;p&gt;pHT = pHNBS + (Δbuffer offset)&lt;/p&gt; &lt;p&gt;where Δbuffer offset was determined daily from Tris-buffer calibration measurements.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Total Alkalinity (TA)&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Raw potentiometric titration files from the Metrohm 877 Titrino Plus were exported using Metrohm tiamo software (version 2.5).&lt;br /&gt; Processing included:&lt;/p&gt; &lt;p&gt;Drift correction and electrode slope verification.&lt;/p&gt; &lt;p&gt;CRM standardization using certified values for TA (Dickson Laboratory, batch number provided in metadata).&lt;/p&gt; &lt;p&gt;Extraction of equivalence point and TA value using open-cell titration algorithms embedded in tiamo.&lt;/p&gt; &lt;p&gt;TA values were reported in µmol kg-1.&lt;br /&gt; If sample density was required, seawater density was calculated using temperature and salinity data following Fofonoff and Millard (1983).&lt;/p&gt; Specified by the Principal Investigator(s) - Loaded ecosys_dat.csv as resource &quot;995155_v1_diel-sampling&quot; in CSV format, treating empty strings and &quot;NA&quot; as missing values, with headers on row 1 - Converted date field from M/D/YYYY format to ISO 8601 YYYY-MM-DD date format - Renamed five fields to valid identifiers: &quot;tank area (m2)&quot; to tank_area, &quot;tank depth (m)&quot; to tank_depth, &quot;flow rate (L sec-1)&quot; to flow_rate, &quot;ta (umol kg -1)&quot; to ta, and &quot;o2 (mg/L)&quot; to o2 - Set data types for all 16 fields: date as date (%Y-%m-%d), experiment and mesocosm and tp as integer, flow_rate, o2, ph_nbs, sal, ta, tank_area, tank_depth, and temp as number, and season, tod, treatment, and type as string - Output written to 995155_v1_diel-sampling.csv Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Snorkel-based Coral Collection Gear Snorkel-based Coral Collection Gear PI Supplied Instrument Name: Snorkel-based Coral Collection Gear PI Supplied Instrument Description:Use: Hand-collection of coral colonies at 1 m depth surrounding HIMB. Notes: Standard snorkeling gear (mask, snorkel, fins) and hand tools (bone cutters, chisels) were used; no specialized electronic instrumentation. Instrument Name: Diving Mask and Snorkel Instrument Short Name: Instrument Description: A diving mask (also half mask, dive mask or scuba mask) is an item of diving equipment that allows underwater divers, including, scuba divers, free-divers, and snorkelers to see clearly underwater. Snorkel: A breathing apparatus for swimmers and surface divers that allows swimming or continuous use of a face mask without lifting the head to breathe, consisting of a tube that curves out of the mouth and extends above the surface of the water. Flow-through Mesocosm System Flow-through Mesocosm System PI Supplied Instrument Name: Flow-through Mesocosm System PI Supplied Instrument Description:Flow-through Mesocosm System Reference: Jokiel et al. 2014 Description: Outdoor continuous-flow mesocosm facility at HIMB consisting of twelve 450 L fiberglass tanks (1 m x 1 m x 0.5 m) supplied by seawater pumped from ~3 m depth in Kāneʻohe Bay. Flow: Adjustable head box delivering ~50 min-1 seawater residence time. Notes: All mesocosms contained two submersible pumps and an airstone for continuous mixing. Instrument Name: no_bcodmo_term Instrument Short Name: Instrument Description: No relevant match in BCO-DMO instrument vocabulary. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/999/ CO2 Gas Delivery System CO2 Gas Delivery System PI Supplied Instrument Name: CO2 Gas Delivery System PI Supplied Instrument Description:Components: CO2 cylinder, regulator, and gas injection line. Use: Manipulation of mesocosm pH by bubbling pure CO2 or CO2-air mixtures directly into individual mesocosms. Calibration: Flow checked daily using bubble-rate timing; regulator pressures recorded each morning. Instrument Name: no_bcodmo_term Instrument Short Name: Instrument Description: No relevant match in BCO-DMO instrument vocabulary. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/999/ Submersible Pumps Submersible Pumps PI Supplied Instrument Name: Submersible Pumps PI Supplied Instrument Description:Manufacturer: Maxi-Jet Model: Maxi-Jet 1600 Use: Internal water circulation and CO2 injection mixing inside mesocosms. Calibration: Flow rate verified twice per season using volumetric bucket timing.   Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps Peristaltic Pumps for TA Manipulation Peristaltic Pumps for TA Manipulation PI Supplied Instrument Name: Peristaltic Pumps for TA Manipulation PI Supplied Instrument Description:Manufacturer: To be filled by user (brand not provided) Flow Rate: 2–3 mL min-1 Use: Delivery of 1.0 M HCl or 1.0 M Na2CO3 to achieve target alkalinity offsets. Calibration: Flow rate calibrated at the start of each 30-day experiment by measuring timed output volumes. Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps Buoyant Weighing Apparatus Buoyant Weighing Apparatus PI Supplied Instrument Name: Buoyant Weighing Apparatus PI Supplied Instrument Description:Use: Measurement of initial and final coral colony mass for calcification estimates. Components: Electronic balance Custom platform submerged in seawater for buoyant weight readings Calibration: Balance calibrated daily using known-weight standards. System checked for drift by repeated weighing of a reference coral fragment. Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/ Open-cell Potentiometric Titrator for Total Alkalinity Open-cell Potentiometric Titrator for Total Alkalinity PI Supplied Instrument Name: Open-cell Potentiometric Titrator for Total Alkalinity PI Supplied Instrument Description:Manufacturer: Metrohm Model: 877 Titrino Plus with 9101 Herisau glass pH electrode Use: Measurement of TA from discrete 100 mL seawater samples twice weekly. Calibration: Daily electrode slope and offset verification. Weekly CRM calibration using certified reference materials from A. Dickson (batch number supplied by user). Notes: All titrations performed at constant temperature. Instrument Name: Titrator Instrument Short Name:Titrator Instrument Description: Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/ YSI Multiparameter Water Quality Meters YSI Multiparameter Water Quality Meters PI Supplied Instrument Name: YSI Multiparameter Water Quality Meters PI Supplied Instrument Description:YSI ProDSS YSI 556 MPS Manufacturer: YSI Inc. Parameters Measured: Temperature, salinity, dissolved oxygen, and pHNBS. Calibration: DO probe calibrated daily using water-saturated air. pH electrode calibrated daily using pH 4, 7, and 10 NIST buffers; corrected to total scale using Tris buffer from Dickson Laboratory. Conductivity and temperature routinely checked using certified standards. Instrument Name: Water Quality Multiprobe Instrument Short Name:Water Quality Multiprobe Instrument Description: An instrument which measures multiple water quality parameters based on the sensor configuration.