http://lod.bco-dmo.org/id/dataset/995462
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2026-03-24
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
CTD profile data from R/V Atlantis cruise AT50-08 in the ETNP oxygen minimum zone
2026-03-24
publication
2026-03-24
revision
BCO-DMO Linked Data URI
2026-03-24
creation
http://lod.bco-dmo.org/id/dataset/995462
Gordon T. Taylor
Stony Brook University - SoMAS
principalInvestigator
Elena Yakubovskaya
Stony Brook University - SoMAS
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Taylor, G. T., Yakubovskaya, E. (2026) CTD profile data from R/V Atlantis cruise AT50-08 in the ETNP oxygen minimum zone. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-03-24 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/995462 [access date]
CTD profile data from R/V Atlantis cruise AT50-08 in the ETNP oxygen minimum zone Dataset Description: Methods and Sampling: <p>Seawater samples (10 × 100 L) were collected during R/V <em>Atlantis</em> cruise AT50-08 (Feb–Mar 2023) at two stations in the Oxygen Minimum Zone of the Eastern Tropical North Pacific (10–600 m depth). Samples were collected by CTD rosette, pre-filtered through 0.22 µm filters to remove cells and debris, and concentrated ~500× by tangential flow filtration (100 kDa cutoff).</p>
<p>The resulting concentrates contained both viruses and extracellular vesicles (EVs). EVs were enriched on board using a lectin-based binding column. Transmission electron microscopy (JEOL JEM 1400, Stony Brook University CryoEM facility) confirmed EV enrichment after column purification.</p>
<p>Subsequent EV analyses were performed at the University of Pennsylvania School of Veterinary Medicine EV Core Facility. Methods included size-based separation (SEC-HPLC, gravity-driven iZon column, density-gradient ultracentrifugation), particle quantification (resistive pulse sensing, nCS1; nanoparticle tracking analysis, NTA), and immunophenotyping (nanoscale flow cytometry; ExoView™ chip array). See related datasets.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2202723 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2202723
onGoing
Gordon T. Taylor
Stony Brook University - SoMAS
631-632-8688
Stony Brook University School of Marine and Atmospheric Sciences
Stony Brook
NY
11794-5000
USA
gordon.taylor@stonybrook.edu
pointOfContact
Elena Yakubovskaya
Stony Brook University - SoMAS
elena.yakubovskaya@stonybrook.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sea-Bird SBE 911plus CTD System
10-L Niskin Bottles
theme
None, User defined
CTD Sea-Bird SBE 911plus
Niskin bottle
instrument
BCO-DMO Standard Instruments
AT50-08A
AT50-08B
service
Deployment Activity
Eastern Tropical Pacific, Minimum Oxygen Zone (OMZ)
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
EAGER: Mechanistic Study of Extracellular Vesicle Production by Marine Microalgae using Advanced Imaging Technologies
https://osprey.bco-dmo.org/project/885370
EAGER: Mechanistic Study of Extracellular Vesicle Production by Marine Microalgae using Advanced Imaging Technologies
<p><em>NSF Award Abstract:</em><br />
This EAGER project is a proof-of-concept study on the composition, origins, and dynamics of extracellular vesicles (EVs) produced by marine microalgae in response to various environmental and biotic stressors. EVs are microscopic lipid-encased particles that are released naturally from almost all cell types and are vehicles for a variety of cargo, including genetic material (RNA, DNA), proteins, and lipids. EVs have been variously postulated to serve as a defense against viral attack, a waste disposal mechanism, a stress response, or a means of cell-to-cell communication. Marine microalgae are pivotal players in the global carbon cycle. By better understanding processes that govern their population dynamics and responses to environmental changes, we can develop better predictive models of responses to global climate change. The need to understand these mechanisms is becoming increasingly urgent as climate change becomes more manifest. Very recent findings suggest that EVs play a key role in marine phytoplankton population regulation, but our understanding of their function(s) in planktonic systems is severely limited and fragmentary. This project addresses significant knowledge gaps and explores the potential complexities of marine planktonic EV production. This project provides support and training to a female graduate and undergraduate marine sciences students, who are receiving unique opportunities to master new experimental approaches and state-of-the-art research tools that are extremely rare in marine sciences programs. The project supports high school students in marine sciences studies as a part of the summer science camp (<a href="http://www.sigmacamp.org" target="_blank">www.sigmacamp.org</a>). A female postdoc is also being trained on the project.</p>
<p>Using the cosmopolitan and geochemically-important microalga E. huxleyi as a model system, this project tests three major hypotheses to enhance our understanding of the purpose(s) of microalgal EV production. (1) Microalgae produce distinctive types of EVs (ectosomes or exosomes) in response to different environmental conditions, and EV types have definitive functions (stress response, viral defense, intercellular communication, waste disposal). (2) EVs’ cargo is diverse, so their production and release reflect a complex intercellular communication mechanism. (3) Exosome genesis is a multistage process, and its stages are separated in time. Therefore, algal cells may contain a pool of pre-formed EVs loaded with different cargo that are stored internally, and when induced by a sudden change in external conditions are released through the outer membrane. To adequately test these hypotheses requires using single particle analytical methods in addition to ensemble measurements. The investigators are using an assortment of recently developed methods and original experimental approaches developed by our group to investigate EV compositional variability under selected stress conditions. They use single particle Raman microspectroscopy, pulse-chase Stable Isotope Probing, and LC-MSMS for compositional analysis of EVs, and Cryo-EM and AFM for morphological analyses. If experimental data confirm our suspicions, then phytoplankton EVs represent a novel and essentially overlooked mechanism of extracellular interactions that potentially govern a wide range of globally-important processes.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Marine Microbial Extracellular Vesicles
largerWorkCitation
project
eng; USA
oceans
Eastern Tropical Pacific, Minimum Oxygen Zone (OMZ)
2023-02-10
2023-03-15
Coastal Western Atlantic waters and Eastern Tropical North Pacific OMZ
0
BCO-DMO catalogue of parameters from CTD profile data from R/V Atlantis cruise AT50-08 in the ETNP oxygen minimum zone
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/995462/data/download
download
onLine
dataset
<p>Seawater samples (10 × 100 L) were collected during R/V <em>Atlantis</em> cruise AT50-08 (Feb–Mar 2023) at two stations in the Oxygen Minimum Zone of the Eastern Tropical North Pacific (10–600 m depth). Samples were collected by CTD rosette, pre-filtered through 0.22 µm filters to remove cells and debris, and concentrated ~500× by tangential flow filtration (100 kDa cutoff).</p>
<p>The resulting concentrates contained both viruses and extracellular vesicles (EVs). EVs were enriched on board using a lectin-based binding column. Transmission electron microscopy (JEOL JEM 1400, Stony Brook University CryoEM facility) confirmed EV enrichment after column purification.</p>
<p>Subsequent EV analyses were performed at the University of Pennsylvania School of Veterinary Medicine EV Core Facility. Methods included size-based separation (SEC-HPLC, gravity-driven iZon column, density-gradient ultracentrifugation), particle quantification (resistive pulse sensing, nCS1; nanoparticle tracking analysis, NTA), and immunophenotyping (nanoscale flow cytometry; ExoView™ chip array). See related datasets.</p>
Specified by the Principal Investigator(s)
<p>CTD data were processed following standard Sea-Bird CTD procedures. Raw measurements were converted to engineering units using manufacturer calibration coefficients, then time-aligned across sensors (e.g., conductivity and oxygen aligned to pressure/temperature) and corrected for conductivity cell thermal mass.</p>
<p>Data were screened to remove periods of unstable flow and package motion (surface soak, pump start-up, winch stops/pressure reversals/loops), and automated despiking (“wild edit”) was applied to remove dropouts and isolated spikes.</p>
<p>Final profiles were generated from the downcast and used to compute derived variables including practical salinity, depth (from pressure using latitude), density, sound velocity (Chen–Millero), and dissolved oxygen products (µmol/kg, mL/L, % saturation); some oxygen fields include a smoothed/windowed version indicated in the headers (e.g., “WS=2”).</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Sea-Bird SBE 911plus CTD System
Sea-Bird SBE 911plus CTD System
PI Supplied Instrument Name: Sea-Bird SBE 911plus CTD System PI Supplied Instrument Description:Sea-Bird SBE 911plus CTD system (SBE 9plus underwater unit with an SBE 11+ deck unit) mounted on a rosette frame with an SBE 32, 24-position carousel carrying 10-L Niskin bottles (Atlantis typically sails with 23 bottles installed, but it can be configured to 24). Instrument Name: CTD Sea-Bird SBE 911plus Instrument Short Name:CTD SBE 911plus Instrument Description: The Sea-Bird SBE 911 plus is a type of CTD instrument package for continuous measurement of conductivity, temperature and pressure. The SBE 911 plus includes the SBE 9plus Underwater Unit and the SBE 11plus Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 plus and SBE 11 plus is called a SBE 911 plus. The SBE 9 plus uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 plus and SBE 4). The SBE 9 plus CTD can be configured with up to eight auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0058/
10-L Niskin Bottles
10-L Niskin Bottles
PI Supplied Instrument Name: 10-L Niskin Bottles PI Supplied Instrument Description:Sea-Bird SBE 911plus CTD system (SBE 9plus underwater unit with an SBE 11+ deck unit) mounted on a rosette frame with an SBE 32, 24-position carousel carrying 10-L Niskin bottles (Atlantis typically sails with 23 bottles installed, but it can be configured to 24). Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: AT50-08A
AT50-08A
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
Cruise: AT50-08B
AT50-08B
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel