http://lod.bco-dmo.org/id/dataset/995690 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2026-03-30 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Boron isotope data from bivalves from 2022 tank experiment in the Gulf of Maine, USA 2026-03-30 publication 2026-03-30 revision Woods Hole Oceanographic Institution (WHOI BCO-DMO) 2026-04-22 publication https://doi.org/10.26008/1912/bco-dmo.995690.1 Diana Lynn Thatcher Iowa State University principalInvestigator Michele LaVigne Bowdoin College principalInvestigator Alan Wanamaker Iowa State University principalInvestigator Branwen Williams Claremont McKenna College principalInvestigator Brittany Jellison University of New Hampshire principalInvestigator Teagan McMahon Claremont McKenna College principalInvestigator Whitney Nina Western Washington University principalInvestigator Joseph Stewart University of Bristol, UK principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Thatcher, D. L., LaVigne, M., Wanamaker, A., Williams, B., Jellison, B., McMahon, T., Nina, W., Stewart, J., Wanamaker, A. (2026) Boron isotope data from bivalves from 2022 tank experiment in the Gulf of Maine, USA. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-03-30 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.995690.1 [access date] Boron isotope data from bivalves from 2022 tank experiment in the Gulf of Maine, USA Dataset Description: &lt;p&gt;The data files that makeup this dataset were generated from a flow-through tank experiment conducted at the Schiller Coastal Studies Center. The experiment was designed to examine physiological or environmental responses of multiple bivalve species under controlled conditions using continuously supplied seawater.&lt;/p&gt; &lt;p&gt;Specimens used in the experiment included both field-collected and hatchery-sourced organisms. Juvenile and adult individuals of Arctica islandica were collected offshore of Jonesport, Maine, USA (44°33.247’N, 67°16.183’W) at depths of approximately 76–85 meters. Juvenile Placopecten magellanicus were obtained from PenBay Farmed Scallops. Juvenile Mercenaria mercenaria and Mya arenaria were provided by the Downeast Institute Hatchery.&lt;/p&gt; &lt;p&gt;Four bivalve species were maintained in a fully factorial design with four pH treatments (~7.4, 7.6, 7.8, ~8.0) and three temperature treatments (~6, 9, 12 °C). Environmental conditions were continuously monitored (temperature) and regularly validated (pH, salinity), with all reported values representing tank-level averages. Specimen-level measurements (size, dry weight, buoyant weight) were collected at defined time points and linked to experimental treatment conditions.&lt;/p&gt; Methods and Sampling: &lt;p&gt;&lt;u&gt;Experimental setup:&lt;/u&gt;&lt;/p&gt; &lt;p&gt;These four species were grown in four pH treatments (~7.4, 7.6, 7.8, and ambient conditions with a pH=8.0) at three temperature treatments (~6, 9, 12° C). There was one tank at each pH for T=6 and 12° C and two tanks at each pH for T=9° C.&lt;/p&gt; &lt;p&gt;Specimens were randomly assigned to tank conditions with roughly equal numbers of each specimen in each tank condition.&lt;/p&gt; &lt;p&gt;Specimens were in the tank conditions for 20.5 weeks before removed with the following exception. Half of the &lt;em&gt;P. magellanicus&lt;/em&gt; were removed in April 2022.&lt;/p&gt; &lt;p&gt;&lt;u&gt;Environmental Conditions:&lt;/u&gt;&lt;/p&gt; &lt;p&gt;pH and salinity were measured weekly with a YSI probe. pH was controlled within each tank condition by the Apex control system. Tanks with lower pH conditions than ambient were obtained by bubbling CO&lt;sub&gt;2&lt;/sub&gt; from compressed cylinders into the water of a mixing chamber before leading to each of the three sets of controlled pH tanks.&lt;/p&gt; &lt;p&gt;Temperature was measured with two Hobo Tidbit devices in each tank throughout the experiment. Temperatures were measured every minute throughout the 20.5 weeks. Temperatures were maintained within the tanks using Inkbird controllers and two (500W/800W) heaters per tank.&lt;/p&gt; &lt;p&gt;All pH measurements are on the total pH scale (pH&lt;sub&gt;T&lt;/sub&gt;).&lt;/p&gt; &lt;p&gt;The reported measurements for temperature, salinity, and pH&lt;sub&gt;T&lt;/sub&gt; are measured tank averages.&lt;/p&gt; &lt;p&gt;&lt;u&gt;Specimen measurements:&lt;/u&gt;&lt;/p&gt; &lt;p&gt;All study specimens were measured for maximum height, dry (live) weight, and buoyant weight at the start and end of the experiment. For the &lt;em&gt;P. magellanicus&lt;/em&gt; removed in April 2022, height, dry (live) weight, buoyant were measured after removal from the tanks. Photos were taken of each specimen at the conclusion of the experiment.&lt;/p&gt; &lt;p&gt;After the conclusion of the experiment, all specimens were dissected, shells were rinsed with DI water and allowed to air dry prior to sampling for boron isotopes.&lt;/p&gt; &lt;p&gt;&lt;u&gt;Shell sampling:&lt;/u&gt;&lt;/p&gt; &lt;p&gt;Organic matter was removed with burring bit (Brasseler #835.11.010) and hand milling with a Dremel tool prior to sampling of the shell. 8 mg of shell was sampled (3-4 mg for P. magellanicus) for boron isotopes with the same bit and Dremel tool.&lt;/p&gt; &lt;p&gt;Boron samples were collected on the shells from the portion of the shell that grew from approximately week 2 to week 10 of the experiment (prior to the spring freshening and second calcein stain line in April 2022) for all species except the adult &lt;em&gt;A. islandica&lt;/em&gt;. Due to much slower growth in the adult &lt;em&gt;A islandica&lt;/em&gt;, the portion of the shell that grew during the entire tank experiment was sampled.&amp;nbsp;&lt;/p&gt; &lt;p&gt;For most shells, one sample was collected from each shell. There were two exceptions: a comparison was done between the two halves of the shell for three juvenile &lt;em&gt;A. islandica&lt;/em&gt; (g5, g32, g48) and a comparison of the outer shell layer (both inner and outer layers of the outer shell) and the inner shell layer on two juvenile &lt;em&gt;A. islandica&lt;/em&gt; (g40 and g52). The samples selected for these comparisons were randomly chosen.&lt;/p&gt; &lt;p&gt;&lt;u&gt;Sample preparation:&lt;/u&gt;&lt;/p&gt; &lt;p&gt;All powdered shell samples were subject to two 15-minute leaches in warm 10% (by volume) H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt; (80&amp;nbsp;°C; buffered in NH&lt;sub&gt;4&lt;/sub&gt;OH) and a weak acid leach (0.0005&amp;nbsp;M HNO&lt;sub&gt;3&lt;/sub&gt;) before powders were dissolved in distilled 0.5&amp;nbsp;M HNO&lt;sub&gt;3&lt;/sub&gt;. Dissolved samples were centrifuged (1 min at 13k rpm), and if there was any visible undissolved non-carbonate material at the bottom of the microcentrifuge tube the dissolved sample was transferred to a clean vial, rejecting the small undissolved fraction.&lt;/p&gt; &lt;p&gt;The burring bit was used to remove all visible organic matter from the outside of the shells. The leaching in H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt; was performed to remove the remaining organic matter.&amp;nbsp;&lt;/p&gt; &lt;p&gt;&lt;u&gt;Chemical Processing:&lt;/u&gt;&lt;/p&gt; &lt;p&gt;All trace element and boron isotope analyses were performed at the University of Bristol. An aliquot of the dissolved sample was analyzed by Inductively Coupled Plasma-Mass Spectrometer (ICP-MS) using well-characterized, matrix-matched, synthetic standard solutions to give B/Ca and U/Ca ratios. Samples and standards were introduced in 0.5&amp;nbsp;M HNO&lt;sub&gt;3&lt;/sub&gt; and an acid wash solution of 0.5&amp;nbsp;M HNO&lt;sub&gt;3&lt;/sub&gt; matrix and 0.3&amp;nbsp;M HF was utilized between samples/standards to aid B wash out.&lt;/p&gt; &lt;p&gt;The remaining sample containing between 4 and 40&amp;nbsp;ng of B was separated from the carbonate matrix using 20&amp;nbsp;μl micro-columns containing Amberlite IRA 743 boron-specific anionic exchange resin. The boron separation from the matrix was performed only on the day preceding Multi Collector-ICP-MS (MC-ICP-MS) analysis. All samples, blanks, and standard solutions were introduced to the instrument in a 0.5&amp;nbsp;M HNO&lt;sub&gt;3&lt;/sub&gt; and 0.3&amp;nbsp;M HF acid matrix again to ensure optimal B wash out.&lt;/p&gt; &lt;p&gt;A total of 108 samples were prepared for boron isotope analysis. There were two samples that did not produce useful results due to blocked columns (one adult &lt;em&gt;A. islandica&lt;/em&gt; and one juvenile &lt;em&gt;P. magellanicus&lt;/em&gt;).&amp;nbsp;&lt;/p&gt; &lt;p&gt;See Thatcher et al., (2026; GCA) and McMahon et al. (2024; PLOS Climate) for full details on the methods.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2028197 Award URL: https://www.nsf.gov/awardsearch/show-award/?AWD_ID=2028197 completed Diana Lynn Thatcher Iowa State University 5157083608 thatcher@iastate.edu pointOfContact Michele LaVigne Bowdoin College mlavign@bowdoin.edu pointOfContact Alan Wanamaker Iowa State University (703)292-7516 adw@iastate.edu pointOfContact Branwen Williams Claremont McKenna College 909-607-8017 W.M. Keck Science Department 925 N. Mills Ave Claremont CA 91711 USA Branwen.Williams@ClaremontMcKenna.edu pointOfContact Brittany Jellison University of New Hampshire 805-338-6610 Spaulding Hall Durham NH 03824 USA brittany.jellison@unh.edu pointOfContact Teagan McMahon Claremont McKenna College tmcmaho@students.pitzer.edu pointOfContact Whitney Nina Western Washington University whitnen@wwu.edu pointOfContact Joseph Stewart University of Bristol, UK joseph.stewart@bristol.ac.uk pointOfContact asNeeded Dataset Version: 1 Unknown SampleID Sample_species Sample_description Average_temperature Average_pHT Average_salinity boron_isotope_value_borate_ion boron_isotope_value_shell boron_calcium_ratio delta_pH Inductively Coupled Plasma Mass Spectrometer (ICP-MS) Inductively Coupled Plasma-Mass Spectrometer (ICP-MS) Mettler Toledo ME204E Ohaus Adventurer AR1140 Onset HOBO TidbiT MX2032 YSI Pro Plus Multiparameter Meter (Xylem Inc.) theme None, User defined sample identification species scientific name (binomial) sample description incubation or cultivation temperature (not ambient water temp) pH salinity Boron ratio featureType BCO-DMO Standard Parameters Inductively Coupled Plasma Mass Spectrometer Multi Collector Inductively Coupled Plasma Mass Spectrometer scale or balance scale or balance Temperature Logger YSI Professional Plus Multi-Parameter Probe instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Using multi-proxy paleo data to constrain natural and anthropogenic hydrographic variability in the Gulf of Maine System over the last 250 years https://osprey.bco-dmo.org/project/995726 Collaborative Research: Using multi-proxy paleo data to constrain natural and anthropogenic hydrographic variability in the Gulf of Maine System over the last 250 years <p>NSF Award Abstract:</p> <p>The Gulf of Maine is located in the Northwestern Atlantic Ocean. Its waters serve as home to the economically important Atlantic Cod and shellfish industry. But recent changes to this environment pose a threat to the ecosystem and the commercial fishing industry. However, only a few long-term records of the environment exist in this area making it difficult to tell exactly when these changes started and how much is related to human activities. In this project, a team of researchers from three universities will construct a 250-year history of the regional environment by measuring yearly growth bands in local clams and algae. Chemical fingerprints in these growth bands will be used to determine the water temperature, salinity, biological productivity and pH conditions. This allow scientists to study how natural changes have affected the environment and compare these to changes caused by greenhouse global warming. This information will inform policy decisions of the newly-formed Maine Climate Council on how to better manage this area. The proposed work will also support training for early career scientists, broaden participation in science programs, and support undergraduate research opportunities.</p> <p>Specifically, the team will use a multi-pronged approach using geochemical proxies in two types of high-resolution marine climate archives (clams and crustose coralline algae) to reconstruct past changes in oceanographic conditions, including near surface seawater temperatures, salinity, and pH in the Gulf of Maine through the last 250 years. Geochemical fingerprints (or proxies) preserved in the annual growth bands of clam shells and skeletons of long-lived marine calcifiers have been used extensively as archives of past ocean conditions. Discrete calcium carbonate layers can be sampled from the growth bands of clams and crustose coralline algae for geochemical analysis, generating proxy archive records. These reconstructions of past environmental conditions fill data gaps prior to instrumental records. Hence, the proposed research will potentially yield several multi-centennial, annually-resolved, absolutely-dated datasets of oceanographic variability in the Northwestern Atlantic, including the Gulf of Maine, prior to the Industrial Revolution, which will be used to evaluate the role of Atlantic meridional overturning circulation, and other climate forcings, on observed changes. By combining new data derived from traditional and novel proxies with existing hydrographic records, the relative contributions of source waters into the Gulf of Maine through time will be characterized to better understand the drivers of variability within the Gulf of Maine. Understanding past oceanographic variability in the Gulf of Maine is therefore critical for predicting the likely extent and magnitude of future change, and for planning to safeguard ecosystems and fisheries. Results will be widely disseminated to science and non-science audiences through publications, conference presentations, mentoring programs, coursework, and outreach activities at area schools, museums, and science centers.</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> GoM Paleo and Tank Experiment largerWorkCitation project eng; USA oceans 2022-01-14 2022-06-08 Gulf of Maine/NW Atlantic Ocean 0 BCO-DMO catalogue of parameters from Boron isotope data from bivalves from 2022 tank experiment in the Gulf of Maine, USA Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/997167.rdf Name: SampleID Units: unitless Description: <p>Name/ID of specimen in sample.</p> http://lod.bco-dmo.org/id/dataset-parameter/997168.rdf Name: Sample_species Units: unitless Description: <p>Species of specimen in sample.</p> http://lod.bco-dmo.org/id/dataset-parameter/997169.rdf Name: Sample_description Units: unitless Description: <p>Sample description.</p> http://lod.bco-dmo.org/id/dataset-parameter/997170.rdf Name: Average_temperature Units: degrees Celsius Description: <p>Average tank temperature of the experiment tank.</p> http://lod.bco-dmo.org/id/dataset-parameter/997171.rdf Name: Average_pHT Units: pH units Description: <p>Average total pH of the experiment tank.</p> http://lod.bco-dmo.org/id/dataset-parameter/997172.rdf Name: Average_salinity Units: psu Description: <p>Average salinity of the experiment tank.</p> http://lod.bco-dmo.org/id/dataset-parameter/997173.rdf Name: boron_isotope_value_borate_ion Units: per mil (‰, water) Description: <p>Boron isotope value of the borate ion.</p> http://lod.bco-dmo.org/id/dataset-parameter/997174.rdf Name: boron_isotope_value_shell Units: per mil (‰) Description: <p>Boron isotope value of the shell. Missing data identifier &quot;Column blocked&quot; within this column indicates where delta pH values were not calculated for this sample.</p> http://lod.bco-dmo.org/id/dataset-parameter/997175.rdf Name: boron_calcium_ratio Units: (umol/mol) Description: <p>Boron to calcium ratio.</p> http://lod.bco-dmo.org/id/dataset-parameter/997176.rdf Name: delta_pH Units: pH units Description: <p>Delta pH. Missing data identifier &quot;**&quot; ** indicates samples where the d11B values are not explainable by pH variability alone. &quot;N/A&quot; values indicate delta pH values were not calculated for this sample.</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact https://doi.org/10.26008/1912/bco-dmo.995690.1 download onLine dataset &lt;p&gt;&lt;u&gt;Experimental setup:&lt;/u&gt;&lt;/p&gt; &lt;p&gt;These four species were grown in four pH treatments (~7.4, 7.6, 7.8, and ambient conditions with a pH=8.0) at three temperature treatments (~6, 9, 12° C). There was one tank at each pH for T=6 and 12° C and two tanks at each pH for T=9° C.&lt;/p&gt; &lt;p&gt;Specimens were randomly assigned to tank conditions with roughly equal numbers of each specimen in each tank condition.&lt;/p&gt; &lt;p&gt;Specimens were in the tank conditions for 20.5 weeks before removed with the following exception. Half of the &lt;em&gt;P. magellanicus&lt;/em&gt; were removed in April 2022.&lt;/p&gt; &lt;p&gt;&lt;u&gt;Environmental Conditions:&lt;/u&gt;&lt;/p&gt; &lt;p&gt;pH and salinity were measured weekly with a YSI probe. pH was controlled within each tank condition by the Apex control system. Tanks with lower pH conditions than ambient were obtained by bubbling CO&lt;sub&gt;2&lt;/sub&gt; from compressed cylinders into the water of a mixing chamber before leading to each of the three sets of controlled pH tanks.&lt;/p&gt; &lt;p&gt;Temperature was measured with two Hobo Tidbit devices in each tank throughout the experiment. Temperatures were measured every minute throughout the 20.5 weeks. Temperatures were maintained within the tanks using Inkbird controllers and two (500W/800W) heaters per tank.&lt;/p&gt; &lt;p&gt;All pH measurements are on the total pH scale (pH&lt;sub&gt;T&lt;/sub&gt;).&lt;/p&gt; &lt;p&gt;The reported measurements for temperature, salinity, and pH&lt;sub&gt;T&lt;/sub&gt; are measured tank averages.&lt;/p&gt; &lt;p&gt;&lt;u&gt;Specimen measurements:&lt;/u&gt;&lt;/p&gt; &lt;p&gt;All study specimens were measured for maximum height, dry (live) weight, and buoyant weight at the start and end of the experiment. For the &lt;em&gt;P. magellanicus&lt;/em&gt; removed in April 2022, height, dry (live) weight, buoyant were measured after removal from the tanks. Photos were taken of each specimen at the conclusion of the experiment.&lt;/p&gt; &lt;p&gt;After the conclusion of the experiment, all specimens were dissected, shells were rinsed with DI water and allowed to air dry prior to sampling for boron isotopes.&lt;/p&gt; &lt;p&gt;&lt;u&gt;Shell sampling:&lt;/u&gt;&lt;/p&gt; &lt;p&gt;Organic matter was removed with burring bit (Brasseler #835.11.010) and hand milling with a Dremel tool prior to sampling of the shell. 8 mg of shell was sampled (3-4 mg for P. magellanicus) for boron isotopes with the same bit and Dremel tool.&lt;/p&gt; &lt;p&gt;Boron samples were collected on the shells from the portion of the shell that grew from approximately week 2 to week 10 of the experiment (prior to the spring freshening and second calcein stain line in April 2022) for all species except the adult &lt;em&gt;A. islandica&lt;/em&gt;. Due to much slower growth in the adult &lt;em&gt;A islandica&lt;/em&gt;, the portion of the shell that grew during the entire tank experiment was sampled.&amp;nbsp;&lt;/p&gt; &lt;p&gt;For most shells, one sample was collected from each shell. There were two exceptions: a comparison was done between the two halves of the shell for three juvenile &lt;em&gt;A. islandica&lt;/em&gt; (g5, g32, g48) and a comparison of the outer shell layer (both inner and outer layers of the outer shell) and the inner shell layer on two juvenile &lt;em&gt;A. islandica&lt;/em&gt; (g40 and g52). The samples selected for these comparisons were randomly chosen.&lt;/p&gt; &lt;p&gt;&lt;u&gt;Sample preparation:&lt;/u&gt;&lt;/p&gt; &lt;p&gt;All powdered shell samples were subject to two 15-minute leaches in warm 10% (by volume) H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt; (80&amp;nbsp;°C; buffered in NH&lt;sub&gt;4&lt;/sub&gt;OH) and a weak acid leach (0.0005&amp;nbsp;M HNO&lt;sub&gt;3&lt;/sub&gt;) before powders were dissolved in distilled 0.5&amp;nbsp;M HNO&lt;sub&gt;3&lt;/sub&gt;. Dissolved samples were centrifuged (1 min at 13k rpm), and if there was any visible undissolved non-carbonate material at the bottom of the microcentrifuge tube the dissolved sample was transferred to a clean vial, rejecting the small undissolved fraction.&lt;/p&gt; &lt;p&gt;The burring bit was used to remove all visible organic matter from the outside of the shells. The leaching in H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt; was performed to remove the remaining organic matter.&amp;nbsp;&lt;/p&gt; &lt;p&gt;&lt;u&gt;Chemical Processing:&lt;/u&gt;&lt;/p&gt; &lt;p&gt;All trace element and boron isotope analyses were performed at the University of Bristol. An aliquot of the dissolved sample was analyzed by Inductively Coupled Plasma-Mass Spectrometer (ICP-MS) using well-characterized, matrix-matched, synthetic standard solutions to give B/Ca and U/Ca ratios. Samples and standards were introduced in 0.5&amp;nbsp;M HNO&lt;sub&gt;3&lt;/sub&gt; and an acid wash solution of 0.5&amp;nbsp;M HNO&lt;sub&gt;3&lt;/sub&gt; matrix and 0.3&amp;nbsp;M HF was utilized between samples/standards to aid B wash out.&lt;/p&gt; &lt;p&gt;The remaining sample containing between 4 and 40&amp;nbsp;ng of B was separated from the carbonate matrix using 20&amp;nbsp;μl micro-columns containing Amberlite IRA 743 boron-specific anionic exchange resin. The boron separation from the matrix was performed only on the day preceding Multi Collector-ICP-MS (MC-ICP-MS) analysis. All samples, blanks, and standard solutions were introduced to the instrument in a 0.5&amp;nbsp;M HNO&lt;sub&gt;3&lt;/sub&gt; and 0.3&amp;nbsp;M HF acid matrix again to ensure optimal B wash out.&lt;/p&gt; &lt;p&gt;A total of 108 samples were prepared for boron isotope analysis. There were two samples that did not produce useful results due to blocked columns (one adult &lt;em&gt;A. islandica&lt;/em&gt; and one juvenile &lt;em&gt;P. magellanicus&lt;/em&gt;).&amp;nbsp;&lt;/p&gt; &lt;p&gt;See Thatcher et al., (2026; GCA) and McMahon et al. (2024; PLOS Climate) for full details on the methods.&lt;/p&gt; Specified by the Principal Investigator(s) - Loaded sheet 1 from &quot;Thatcher et al_summary data.xlsx&quot; in xlsx format, using row 1 as header, with missing values defined as empty strings, &quot;nd&quot;, &quot;**&quot;, &quot;Column blocked&quot;, and &quot;N/A&quot;. These various missing data values are retained within the dataset to differentiate between types of data flags, as they differ between columns. - Renamed fields: &quot;Average temperature (°C)&quot; to Average_temperature, &quot;Average salinity (psu)&quot; to Average_salinity, &quot;Average pHT&quot; to Average_pHT, &quot;Sample species&quot; to Sample_species, &quot;Sample description&quot; to Sample_description, &quot;δ11B Borate (‰, water)&quot; to boron_isotope_value_borate_ion, &quot;δ11B (‰)&quot; to boron_isotope_value_shell, &quot;B/Ca (µmol/mol)&quot; to boron_calcium_ratio, &quot;ΔpH&quot; to delta_pH - Set data columns to the following data types: Average_temperature, Average_salinity, Average_pHT, boron_isotope_value_borate_ion, boron_isotope_value_shell, delta_pH as number; boron_calcium_ratio as integer; SampleID, Sample_description, Sample_species as string - Renamed resource from &quot;thatcher_et_al_summary_data-1&quot; to &quot;995690_v1_bivalve_boron_isotopes&quot; - Output written to 995690_v1_bivalve_boron_isotopes.csv Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Inductively Coupled Plasma Mass Spectrometer (ICP-MS) Inductively Coupled Plasma Mass Spectrometer (ICP-MS) PI Supplied Instrument Name: Inductively Coupled Plasma Mass Spectrometer (ICP-MS) PI Supplied Instrument Description:A Inductively Coupled Plasma Mass Spectrometer (ICP-MS) was to quantify elemental ratios (e.g., B/Ca, U/Ca) from dissolved shell samples using matrix-matched standards. Instrument Name: Inductively Coupled Plasma Mass Spectrometer Instrument Short Name:ICP Mass Spec Instrument Description: An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB15/ Inductively Coupled Plasma-Mass Spectrometer (ICP-MS) Inductively Coupled Plasma-Mass Spectrometer (ICP-MS) PI Supplied Instrument Name: Inductively Coupled Plasma-Mass Spectrometer (ICP-MS) PI Supplied Instrument Description:An aliquot of the dissolved sample was analyzed by Inductively Coupled Plasma-Mass Spectrometer (ICP-MS) using well-characterized, matrix-matched, synthetic standard solutions to give B/Ca and U/Ca ratios. Samples and standards were introduced in 0.5 M HNO3 and an acid wash solution of 0.5 M HNO3 matrix and 0.3 M HF was utilized between samples/standards to aid B wash out. Instrument Name: Multi Collector Inductively Coupled Plasma Mass Spectrometer Instrument Short Name:MC-ICPMS Instrument Description: A Multi Collector Inductively Coupled Plasma Mass Spectrometry (MC-ICPMS) is a type of mass spectrometry where the sample is ionized in a plasma (a partially ionized gas, such as Argon, containing free electrons) that has been generated by electromagnetic induction. A series of collectors is used to detect several ion beams simultaneously. A MC-ICPMS is a hybrid mass spectrometer that combines the advantages of an inductively coupled plasma source and the precise measurements of a magnetic sector multicollector mass spectrometer. The primary advantage of the MC-ICPMS is its ability to analyze a broader range of elements, including those with high ionization potential that are difficult to analyze by Thermal Ionization Mass Spectrometry (TIMS). The ICP source also allows flexibility in how samples are introduced to the mass spectrometer and allows the analysis of samples introduced either as an aspirated solution or as an aerosol produced by laser ablation. Mettler Toledo ME204E Mettler Toledo ME204E PI Supplied Instrument Name: Mettler Toledo ME204E PI Supplied Instrument Description:Used to measure dry (live) weight of specimens at the beginning and end of the experiment. These measurements contribute to growth and condition metrics in the dataset. Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/ Ohaus Adventurer AR1140 Ohaus Adventurer AR1140 PI Supplied Instrument Name: Ohaus Adventurer AR1140 PI Supplied Instrument Description:Used to measure buoyant weight of specimens at the start and end of the experiment. Buoyant weight measurements are used to estimate calcification and shell growth. Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/ Onset HOBO TidbiT MX2032 Onset HOBO TidbiT MX2032 PI Supplied Instrument Name: Onset HOBO TidbiT MX2032 PI Supplied Instrument Description:Deployed in duplicate within each tank to record water temperature at 1-minute intervals throughout the 20.5-week experiment. Dataset temperature values represent tank-averaged time series derived from these measurements. Instrument Name: Temperature Logger Instrument Short Name: Instrument Description: Records temperature data over a period of time. YSI Pro Plus Multiparameter Meter (Xylem Inc.) YSI Pro Plus Multiparameter Meter (Xylem Inc.) PI Supplied Instrument Name: YSI Pro Plus Multiparameter Meter (Xylem Inc.) PI Supplied Instrument Description:Used to collect weekly spot measurements of pH and salinity in each tank. These measurements provide calibration/validation for controlled pH conditions and are reported as tank averages in the dataset. Instrument Name: YSI Professional Plus Multi-Parameter Probe Instrument Short Name:YSI ProPlus Instrument Description: The YSI Professional Plus handheld multiparameter meter provides for the measurement of a variety of combinations for dissolved oxygen, conductivity, specific conductance, salinity, resistivity, total dissolved solids (TDS), pH, ORP, pH/ORP combination, ammonium (ammonia), nitrate, chloride and temperature. More information from the manufacturer.